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Bacteria in the milk of sheep with or without mastitis- mini Review
47-52Views:664From a nutritional point of view, sheep milk is more valuable than cow and goat milk and the interest for sheep milk is increasing in many countries. However, sheep milk is easily contaminated during milking, handling, and transport and it is an ideal medium for bacterial propagation. Consequently, sheep milk spoils quite quickly. The proper, clean handling of milk is not only of sanitarian interest, but it also serves the farmers’ interests, because contaminated milk may not be distributed, and is unsuitable for producing good quality products. Following this technological trend, this review addresses the bacterial composition of sheep milk with and without mastitis. Even though sheep milk contains a lot of bacteria, this review article highlighted total plate count, Enterobacteriaceae, coliform, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Campylobacter, Salmonella spp. and Streptococcus spp. Mastitis in sheep is a vital cause of mortality, reduction in milk production and early culling. The reported risk factors for mastitis in sheep were age, a case of mastitis, breed, husbandry systems, and location. The main priority should be implementation of programs to minimize human pathogenic bacteria and mastitis in raw ewe milk.
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The impact of fertilisation on a few microbiological parameters of the carbon cycle
45-50Views:486The 30 years old long-term experiment of Látókép is continued in our experiments. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the microbial processes under nonirrigated and irrigated conditions in maize mono, bi, and triculture. The experimental results and those statistics suggest that the bi and triculture influenced higher microbial activity which was reflected in number of fungus, soil respiration, and microbial biomass carbon (MBC).
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Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:489Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Effect of arsenic treatments on the element content of green peas
203-208Views:574The agricultural environment is contaminated with heavy metals and other toxic elements, which means more and more threats. One of the most important toxic element is the arsenic (As).
The objective of the study was to investigate the effect of As-treatments on the element content of the different parts of the green peas (root,stem, leaf, pod, pea) in the 4. phase of the plant development. Plants were grown in green house. Arsenic was applied in a form of arsenate (As[V]) and the plants were treated with 0, 3, 10, 30, 90 and 270 mg kg-1 arsenic.
According to the results the Ca content of root and pod was increased in the case of the 3 mg kg-1 As-treatment, after that decreasing tendency was observed. In the case of the 270 mg kg-1 As-treatment, the Ca content in the root was increased, because some element is able to concentrate in the lower biomass. The Ca-content of stem and leaves was reduced when the plants were treated with more than 30 mg kg-1 As. The lowest As-treatment (3 mg kg-1) increased the Na content in the root, stem and leaves, however in the case of the higher As-dose, decreasing tendency was observed. In the case of the generative plant parts the 3 mg kg-1 As-treatment also increased the Na content, nevertheless in the case of the higher As-treatments lower Na content was measured, however in the case of the highest As-treatment (270 mg kg-1) the Na content was increased in the generative plant parts, probably the Na was concentrated in the lower biomass. In the case of the 90 and 270 mg kg-1 As-treatment the Mo-content also was increased in the generative plant parts. The 270 mg kg-1 As-treatment caused a similar tendency in the case of the generative plant parts as a result of the lower biomass. In the case of the pod and leaves, the lower As-doses did not cause significant changes.
The Mo content was increased in the root and pea when the plants were treated with 3 mg kg-1 As, but in the case of the higher treatments it was decreased. In the case of the stem it was reverse, the lowest As-tretament (3 mg kg-1) decreased, nevertheless the further As-doses increased the Mo content. -
The possibility of use of the 0,01 M CaCl2 and Baker- Amacher extractants for the determination of plantavailable potassium
7-15Views:194The Hungarian fertilizing recommendation systems use AL soil test for the evaluation of potassium supply. The 0.01 M CaCl2 is a definitely milder extractant, it extracts the easily soluble and exchangeable potassium amount. Its European introduction was already taken into consideration in 1994. The research project on this topic is started in several european countries, also in Hungary at the Department of Agricultural Chemisty of Agricultural University of Debrecen. Another advantage this multielement method is that the different element-ratios can also be calculated.
The Baker-Amacher extractant’s principle is that it contains a known amount of K, P, Mg in the CaCl2 solution. During the soil extraction adsorption and desorption process take place, so the adsorption or desorption can be calculated from the original and the final concentrations.
In this paper we introduce the results of comparing analysis of the samples (n=630) from Soil Information and Monitoring System. Our aim was to measure the use of new extractants beside conventional extractant (AL) for the evaluation of K-supply would be reasonable.
It can be stated that there is a medium close relationship (r=0.75) between AL-K and 0.01 M CaCl2-K. My calculations confirmed the results of former examinations, and proved that the two extractants don’t extract and change the same rate of K-fractions. We found that regression between 0.01 M CaCl2 and AL depend on texture classes, pH classes, amount of lime, and organic matter content of soils.
Comparing the relations between AL and Baker-Amacher we find relatively loose correlation (r=0.45). We stated that there are K-fixing soils among soils considered to be well supplied with potassium by AL. This might be caused by the high amount of mineral clay and the quality of mineral clay. We stated that the dK averages show that the Hungarian nutrient-supply categories characterize generally well K-supplement of soil.
It can be stated that it would be necessary to use new extractants to specify evaluation of plant available K. We found that the 0.01 M CaCl2 and Baker-Amacher extractants could complete usefully the AL procedure and could help effective potassium fertilization. -
The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:360he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:469Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:555Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Opportunities for improving the nutritional value of cereal-based products
275-278Views:893We set up experiments for improving the nutritional value of cerealbased products. Our aims were to decrease the energy content of bakery products with the mixing of plant originated raw materials and byproducts with high fibre contents, and we have evaluated the effect of sodium-chloride on the physical properties of bakery products. We found that the apple pomace, the byproduct of juice production, is excellent for increasing of fibre content of bakery products and the further aim of investigation is to develop economical ways of hygienic byproduct handling and purification. Our experiments, evaluate the bakery use of triticale, have significant achievements and the breads made from triticale flour and whole-grain are commercially available nowadays. Our investigations included the possibilities of decrease of toxin contamination of cereals and our results can form a part of risk estimation systems after further experiments .
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Rippl-Rónai’s color in the native parks: production and using of new, synthetic Wild sage color-mixture
59-64Views:362The climate changes is becoming more damaging to ornamental plants. Besides ornamental species and varieties of plants on public spaces. It will be necessary to use domestic, well adaptable climate-tolerant plant species.
One field of our growing ornamental plants researches from 2001 in the Centre of Agricultural Sciences of University of Debrecen, is studying drought patient, mainly Hungarian improved annual varieties, which are able to get acclimatized with the landscape. Moreover, we have in view to work out new, economical seedling production technologies. In the program, the excellent drought- and frost tolerant annuals and perennials belongs to the climate change tolerant plants. From the evaluated of species we want to create and spread the application. of the new types of ornamental horticulture culture.
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Effect of different lysine-metabolized energy ratio on performance of meat-type geese
39-42Views:548The aim of the experiment was to determine, how the different ratios of the digestibility lysine/ metabolized energy (0.82, 0.87, 0.91 g DLYS/MJ AMEn) of the feed influence the performance of geese. There were 150 goslings (3 treatments, 5 cages/treatments, 10 birds/cages). The experiment has started and finished at the age of 3 and 9 weeks, respectively.
The results of the experiment showed, the different ratios of DLYS/AMEn (0.82, 0.87, 0.91 g DLYS/MJ AMEn) did not influence the performance of young geese. Better growth performances were found for the 0.91 g DLYS/AMEn feed (feed intake, body weight gain, feed and energy conversion rate). This treatment coincided with high feeding costs. Based on these results we need more models and farm experiments to prove this tendency. -
Leaf reflectance characteristics and yield of spring oat varieties as influenced by varietal divergences and nutritional supply
29-34Views:273Inadequacy of nutrients in the soil and sufficient inaccessibility to nutrients is caused by factors that affect production and productivity of spring oat varieties. Exogenous application of nutrient and real time nutrient assessment can therefore reverse these associated negative consequences. A field experiment was conducted to evaluate the response of spring oat varieties to sulphur and silicon based fertilisation. Eight spring oat varieties, four level of nutrient application was arranged in a split-plot design with three replications. The obtained results showed that foliar application of sulphur improves the grain yield of most nutrients responsive varieties by about 34.7%. However, application of silicon had shown a diminishing return association to grain yield of variety GK Kormorán, GK Pillangó, Lota, Panni. LAI, thousands grain weight, SPAD, NDVI was significantly (p < 0.05) influenced by genetic difference of the tested varieties, developmental plasticity, and nutrient application. Significantly higher grain yield was obtained from the variety Mv Pehely than the other tested varieties. Therefore, it could be inferred that a combined use of nutrient responsive spring oat varieties and sulphur containing fertilisers could be important agronomic practice to improve grain yield and to develop climate resilient oat varieties.
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Determining elements of variety-specific maize production technology
157-161Views:302Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
Composition and role of hoverfly (Diptera: Syrphidae) communities in an organic apple orchard
57-61Views:204The use of hoverfly (Syrphidae) larvae in biological control is gaining more attention where the damage by their prey, aphids is significant, especially in agricultural fields and orchards, where they lower the number of aphids. The present study focuses on one hand on faunistics, on the other hand on testing the different available collecting methods for later research. Collecting in an organic apple orchard near Debrecen yielded 525 specimens of Syrphidae mostly by netting but also with white tray traps. Collected species are differentiated according to their feeding (94.1% aphidophagous). The three species found in greatest numbers are Sphaerophoria scripta (Linnaeus, 1758), Melanostoma mellinum (Linnaeus, 1758) and Episyrphus balteatus (De Geer, 1776). The available information on their biology is reviewed and their potential value of hoverflies in biological control is discussed.
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The effect of different bacterial fertilizers on the AL-soluble P2O5 content of soil, and the biomass of the rye-grass (Lolium perenne, L)
93-98Views:525In pot experiment the effect of different bacterial fertilizers on some soil properties, and the amount of plant biomass were studied. The
experiment was set up in 2010 at the Department of Soil Science and Agricultural Chemistry, in a three replications in a random block design. The ryegrass (Lolium perenne, L.) was used as a test plant. The studied soil type was calcareous chernosem soil from Látókép. In our laboratory AL-soluble P2O5 content of soil, the phosphatase enzyme activityof soil, the dry weight of rye-grass, and the phosphorus content of rye-grass were determined.
The results of the study were the following:
– The bacterial fertilizers - by basic treatments NPK - had significant positive effect on the AL- soluble phosphorus content of the soil.
– The soil phosphatase enzyme activity was increased in all cases strongly by the microbial preparations used, the greatest impact was the Bactofil A bacterial fertilizer.
– The plant educed P values significantly increased by the effect of microbial products, in addition to the fund NPK. In this case, the EM-1 and Microbion UNC bacterial fertilizer were the effective.
– In case of the rye-grass biomass none of the bacterial preparations used caused any significant changes, either alone or when used them with straw treatment. -
HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:658Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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Preliminary studies to evaluate the use of spectral data in monitoring of apple orchard parameters
37-41Views:410The introduction/application of precision agricultural technologies has more important role in various fruit growing sectors among others apple growing. Remote sensing methods can detect electromagnetic waves where the green colour of the leaf is responsible for the chlorophyll content. The absorption of chlorophyll is in the wavelength range of 450–670 nm. Samples of apple tree leaves were taken on a weekly basis from the apple orchard at Horticultural Unit of Pallag on University of Debrecen in 2019 summer. Our studies were performed on 2 cultivars (Early Gold, Golden Reinders) and the samples were processed using 2 methodologies: a non-destructive spectral method and spectrophotometric method chlorophyll and carotenoid contents were calculated, which were created into some groups and compared with the spectral values. When the plant begins to lose strong green colour and turns yellow spectral measurements show that chlorophyll content decreases as the proportion of chlorophyll-carotenoid in the plant changes. In case of grouping into intervals, it can be observed that as the chlorophyll content increases the reflectance value decreases continuously due to the strong absorption. Based on the results, close relationship between the pigments can be detected.
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Studying the reproduction-biological effect of the GPR54 receptor gene in sheep
48-51Views:393One of the main economical problems of sheep breeding is that continual production is not possible due to the seasonality of animals. Today, genes, mutations that may develop aseasonality in sheep are extensively researched in order to make the establishment of populations capable of aseasonal estrus possible.
According to the current knowledge, the GPR54 gene participates in the GnRH release from the hypothalamus, and thus in the alteration of the reproduction state of the organism. Our research is aimed to determine whether the GPR54 gene has allele variations that influence the proneness to aseasonal estrus in sheep in a different extent. Therefore, the GPR54 gene sequence of sheep is first examined. -
The noise monitoring system of Debrecen International Airport and requirement of appointing noise protection zones, regarding to the growing air traffic
191-194Views:251The Debrecen International Airport has been undergoing a significant transformation regarding to the growth of traffic and its composition. Considering the dwelling zone location of the airport we cannot avoid the possible noise pollution that it may cause, since its permanency, and growth of intensity, could be the reason of durable health deficiency. The airports’ noise pollution zones are determined, and classified by domestic, and international regulations, the measured data of the noise monitoring system, and the content of the air traffic regarding to the type of planes. Summoning all of these we recommend the D zone qualification of the airport, and the traffic daylight restriction referring to the S5 type of planes. We also advise to build up a more sophisticated and wider extended noise monitoring system.
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Analysis of the situation of domestic game damage and forage ground management
5-8Views:366Game damage is an important problem in home wildlife management, because game managers pay more and more money for game damage year by year. Crop fields can do an appreciable part in reduction of game damages. The aim of the research is to survey the situation of domestic game damage and wildlife forage ground management. (Which are the most used plants on crop fields, how current are the using of seed mixtures, which are the most typical game damages, as well as which are the most applied game control methods?)
It can be stated that the number of plant species sown on crop fields is quite low. Mostly corn and alfalfa are raised, though besides those many other plants are suitable to grow on crop fields. Rising value of game damage and big proportion of the agricultural game damage are well traceable from the questionnaires, too.
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Sour cherry seed as an industrial gamma tocopherol source
27-33Views:386In our experiments sour cherry kernels were investigated. The kernels of different sour cherry cultivars were originated from Újfehértói Gyümölcstermesztési Kutató és Szaktanácsadó Nonprofit Közhasznú Kft. Tocopherol profile, oil yields and nutritional value were stood in focus of examinations.
We started processes preparing of sour cherry kernels. After the preparation recovered non-polar fraction was investigated. We worked with formerly developed chromathographic system, which is capable to separate different tocopherol isomers efficiently. We measured concentration of each tocopherol isomers and registered those and their relation in different oil samples.
According to received data, alpha-, gamma- and delta-tocopherol appeared in samples, but beta-tocopherol could not be detected. It became clear that level of gamma-tocopherol is far higher than alfa- or gamma-isomer, it is concentration exceeded 1–1.5 mg ml-1.
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The effect of grazing of various cattle breeds on botanical composition of low-lying pasture in Hortobágy
57-63Views:677Coenological surveys were conducted in the Hortobágy National Park (Pap-ere and Zám-puszta) in May 2015 and 2016. During the tests,a total of 40 permanent plots were analyzed on grasslands grazed by extensive cattle (Hungarian Grey) and mixed genotype intensive cattle. The presence of plant species, percentages of total coverage of species and vegetation cover were recorded. Two habitat types were chosen according to their moisture content: wet salt marsh meadow (Bolboschoenetum maritimi) and drier salt meadows (Beckmannion eruciformis).
We compared the impact of increased number of animals (2016 years) and the low number of animals (2015 years, initial state) and the grazing exclusion on vegetations.
We tested: (i) what is the impact of grazing on the vegetation, (ii) how do species composition and vegetation charachteristics differ in the two habitat types (iii) and is there a difference in the impact of different cattle breeds (Hungarian gray, intensive beef cattle) grazing on the grasslands species composition? During the investigation we found, (i) that the greatest number of species was recorded in 2015, on the area that received moderate to intensive grazing (14.3 species per m2). Somewhat the number of species was reduced in 2016 due to more intensive grazing. The control group had the lowest number of species (11.7 species per m2). The undergrass and legumes cover significantly increased on intensive grazed lands. (ii) Our results indicate that the effects of different grazing differ in the two studied habitat types. On the drier grasslands greater number of species were found (16.2 species per m2), oppositely to the wet grassland (11.2 species per m2). The cover of the undergrasses was higher in the drier habitat than in the wet. (iii) The extensive beef cattle left a bigger number of species (16 species per m2) than the intensive beef cattle (11.4 species per m2). The grass cover was more intense on areas grazed by intensive cattle. The absolute and potential weeds cover showed a higher value on areas grazed by Hungarian Grey. Our two-year results suggest that grazing by both extensive and intensive cattle breeds can be a proper tool for the conservation management of alkali grasslands. -
Marginalisation and Multifunctional Land Use in Hungary
50-61Views:193Our study prepared as a brief version of National Report in the frame of EUROLAN Programme. We deal with the interpretation of some definitions (marginalisation of land use, multifunctionality of land use, marginalisation of agriculture, multifunctionality of agriculture), with sorting and reviewing indicators of marginalisation and finally with the analysis of functions of land use. We suggested a dynamic and a static approach of marginalisation. We can explore the dynamic process by time series and the static (regional) one by cross-section analyses.
It is very hard to explain the perspective of the future of marginalisation of land and of agriculture in Hungary. The process of marginalisation seems faster in the agriculture in the coming years, but it depends on the utilisation of new possibilities given by the EU financial resources and by the Common Market. At this moment agriculture seems one of the big losers of the accession.
In the long term we should face considerable challenges in the land use. It is necessary to take into account that there is a supply market of foods and traditional fibre production world-wide. There are limited possibilities to produce and to market for example biodiesel (fuel), bioethanol, or maybe biogas. Thus the environment and landscape preservation becomes more and more real land use alternatives.
The environmental interpretation of the multifunctionality of land use: activities (functions) of environmental preservation and nature conservation in a certain area, which aim to preserve natural resources by the existing socio-economic conditions.
Preservation of rural landscapes is the task mainly for land-users, who can be commanded by legal means and can be encouraged by economic measures to carry out the above activity. In the recent past measures of „command and control” type regulation were predominant, however nowadays, especially in the developed countries, the role of economic incentives increases.
As a conclusion of our analysis we can state that as long as the main land-dependent activities (agriculture, forestry, housing, tourism, local mining) cease to be viable under an existing socio-economic structure, then it is hardly possible to sustain the rural landscape on an appropriate level by non-commodity products (such as environment preservation, cultural heritage, nature conservation, employment etc.).
1 The study was prepared in the frame of EUROLAN (EU-5 Framework Project), QLK5-CT-2002-02346, as a compiled version of the Hungarian National Report, The national project co-ordinator: Prof. Dr. Gabor Szabo.
A part of places with high ecological values coincides with the areas with unfavourable agricultural endowments and underdeveloped micro-regions. We think so that the marginalisation preserves the non-environmental-sound activities and hinders the development of multifunctional agriculture and this process can change only by joint utilisation of endogenous and exogenous resources and methods. Thus the successful programmes for agri-environmental protection and multifunctional land use can serve the moderation of negative effects of marginalisation or maybe the marginalisation process itself. -
Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:395The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. -
The methodology of the Hooded Crow’s (Corvus cornix L.) colour ringing and the previous results
43-48Views:366The Hooded Crow (CorvuscornixL.) have moved in several Hungarian cities in the last few decades. It is breeding in Debrecen since 1959, and nowdays it’s presence can be detected at all points of the city, it is an permanent breeding species of the bird fauna. Our knowledge about the nature of urban races, including motion patterns, area fidelity is sufficiently incomplete. The aim of our research is to answer these questions by using our colour-ring program. In this study we present in details the methodology of Hooded Crow’s colour-ring program in urban area, and we also report our previous
achievements.