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Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:428The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
Data on the bumblebee assemblages (Apidae: Bombus spp.) lives in lands under agri-environment commitment
31-35Views:480The goal of agri-environmental schemes (AES) and greening programs are protecting and increasing biodiversity in agricultural lands. The evaluation of effectiveness of AES needs further investigations. For the purpose of investigations, species and species groups should be selected which can indicate the effects of changes in landscape use on biodiversity. Bumblebees are good indicators for this purpose.
The role of bumblebees in pollination is well studied but in the case of different crops, much less detailed data are available. In 2018, bumblebee assemblages of 44 sites belonged to 8 different agricultural and semi-natural habitat types were studied in the surroundings of Sajószöged, Tiszaújváros and Derecske.
This study provides new distribution data of 8 bumblebee species in three 10×10 km UTM cells covering the sampling area. According to our results, the alfalfa and red clover fields and semi-natural grasslands has more species rich and abundant bumblebee assemblages than different crop fields (sunflower, oilseed radish and vegetable morrow) and can help protect bumblebee assemblages of agricultural lands. Based on the collected distribution and abundance data, the role of the bumblebees in pollination of the studied crops should be re-evaluated.
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Basic Research for the Development of Fertiliser Spreaders
52-57Views:225The knowledge of the physical characteristics of fertiliser particles is essential for the constructors and operators of fertiliser distributors. Among physical characteristics, the most important are the frictional and aerodynamic properties for the description of particle movement. Adjustable angled slopes, shearing boxes and various rotating disks are used to identify frictional properties. We have developed a high precision shearing box with digital force measuring cells and a distance signaller (incremental transducer) that we use for slide tests efficiently. We measured the frictional characteristics of 6 different fertilisers: the inner coefficient of friction and the coefficient of friction on ten test surfaces most commonly used in machinery, and we specified the relationship between displacement, loading and the coefficient of friction. We can conclude that the material of the frictional surface significantly influences the force of friction.
However, our experience tells us that the shearing box is not suitable for the measurement of the inner friction, since the examined particles slide on the metal surface of the shearing box in a growing extent in the course of displacement, so it does not measure the real inner friction. Therefore, in our experiment we have developed rotating shearing equipment with a constant shearing surface to identify the inner friction. We tested the equipment with fertilisers and we identified the inner frictional characteristics of 6 different fertilisers. With the developed rotating shearing apparatus we could measure the real inner friction of the particles.
To identify the aerodynamic characteristics of granules, wind tunnels and free-fall tests are used. An elutriator have been developed for our investigation. We have used fertilisers for testing the measuring equipment and we have identified the aerodynamic characteristics of 6 different fertilisers. -
Penicillium chrysogenum antifungal protein (PAF) production in transgenic tobacco (Nicotiana tabacum) plant
77-82Views:264Under the „Molecular farming” research program (product vaccines and substances for medical use with gene manipulated plant) in 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture Department of Plant Biotechnology experiments were launched to transform tobacco plant by PAF antifungal protein. Our aim was to learn the transformation technics. We chose the
Nicotiana tabacum and PAF as model systems.
Our work was to express several different paf constructions in plants with nuclear and plastid transformation too. After that we confirmed the presence of paf gene in the level of DNA and RNA. -
Identification of Hucul mare families by mtDNA markers
75-79Views:621Hundred animal species have disappeared during the last century. By this time, approximately one-third of domestic animals have been in the endangered category. Hucul horses are also in this category; furthermore saving the genetic diversity beside the race preservation is an important challenge as well. The number of mares and stallions is only one of the expressive elements of genetic diversity; together with their quality determine the genetic variability of this breed. Beyond that, if an exact breed can originates from more founders, it can be more renewed genetically. Stud book documents these data by registering the mare families and stallions’ genealogical lineage. Molecular genetics, especially mitochondrial DNA analysis can make the precise identification of mare families possible. As a result of these molecular based methods, protection of genetic diversity, as well as breed preservation became more reliable. After the primer designing, the optimal primer pair was chosen which targets a 1092 bp length DNA sequence in the cytochrome b region. After the successful PCR optimalisation, we determined 170 Hucul mares’ sequences. According to our results, the samples compose ten haplotypes, which are much less, than the registered number of mare families in the stud book. Further investigations are needed to reach more representative results, and drawn the further consequences.
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The change of the inner content parameters of table beet (Beta vulgaris L. ssp. esculenta Gurke var. rubra L.) as a result of storage
113-119Views:453The stored vegetables play a key role in the vegetable consumption. After harvesting, the storage conditions greatly affect nutritional parameters of the crop. The red beet is a root vegetable, which is manufactured mainly fresh in our county, therefore we do not have enough information about the storage. In our experiment, 5 varieties (Rubin, Akela, Larka, Libero, Mona Lisa) were examined in prismatic structure (0–5 °C and 95–97% RH) through 5 mounths.
Our measurements proved that under the appropriate storage conditions water loss was also occurs, which increased the dry matter content (average of 0.5 g 100 g-1). The bioactive compounds, like pigments (betanine and vulgaxanthin) and the quantity of flavonoids, were significantly increased during the experiment.
The results showed that the nutritional parameters of the 5 types was positive during the prismatic structure. However, this storage method negatively affected the bioactive compounds of the Akela genotype. Further studies are recommended for proper storage parameter adjustment, which is justified by the need of the different varieties. The optimal conditions enable us to preserve and to increase the best nutritional index.
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The Natural, Social and Economic Conditions and Opportunities for Development of Balmazujvaros, Especially in the Case of the Co-operative Kvaliko
77-85Views:245Balmazujvaros as a settlement near Hortobagy has to cope with both advantages and disadvantages. Its natural and social conditions are mainly given, the agricultural characteristic is dominant, and the number of the employed is the highest in the agricultural firms and processing industry. Developing the third sector, including hosting, tourism, eco-tourism, thermal-tourism, may be an opportunity for the city. The subsidy for rural development relating to the SAPARD-programme may contribute to this, as the city is the member of the Association of Hajdu Towns.
The agriculture plays an important part in Balmazujvaros in which production and marketing of vegetables and fruits excel. Several farmers realised this opportunity and founded the Marketing Co-operative of Vegetable Producers in Eastern Hungary, that is the Kvaliko. This PO works in a Corporation form and is constantly developing, spreading and expanding its choice making use of the local natural, social and economic conditions and applications for realising its investments. This PO may set an example for the other entrepreneurs and farmers of Balmazujvaros to co-operate and it may be considered as an already realised opportunity. -
Public finance resources and regional distribution of rural development
73-77Views:804Rural development, more specifically the economic development of counties have three public finance resources: the municipality, the central budgetary and the labour market fund resources. Based on the provisions of public finance, there are currently five provisions of the municipality resources of rural development: supporting settlements struggling with employment problems, supporting multi-purpose small regions, supporting settlements which are in a difficult position through no fault of their own, supporting public utility development and supporting holiday resorts. The opportunity of increasing investments is using EU funds. The structural change has a favourable direction, the proportion of the economic development program and regional developments increased in the recent years, but their share is still low. The proportion of agricultural and rural development programs which are important from the aspect of rural development is a less favourable phenomenon. Furthermore, the share of human and social development somewhat decreased, but it is still high. Apart from the regional programs, the Budget Act does not specify the regional distribution of budgets, only the professional purposes of uses, of which it is possible to conclude to which county development can be requested resources for.
Between 2008 and 2011, the 13–15% share of the North Great Plain region was close to the population share of 14.7%. In 2012, there was a turn of events in which the necessity of developing the region was shown. In this year, not only the proportion, but also the absolute extent of development resources increased. Therefore, the region could use resources of 16.9 billion HUF in 2010, 31.4 billion HUF in 2011 and 56.3 billion HUF in 2012. In order to properly use these resources, determined utilisation structure is necessary to be performed. The distribution of development purposes provides an explanation for the backward position of the economic performance of Hajdú-Bihar county.
Of the development resources available for four years, the region spent only 12.2% on economic development.
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Methane emission from Matsuo rice paddy field in light of different fertilizers, costs, profit and carbon credit
9-13Views:283Nowadays global warming is a major issue to our environment. This issue is generated by the modern human activities like industry and intensive agriculture. This research is about methane emission from rice paddy fields. The aim of the study is to lower the methane emission from the field with the help of using different type of fertilizers, whilst we keep in focus the efficient economic operation. The main experimental field is Matsuo paddy field, (Matsuo town, Sanbu city, Chiba prefecture) which is analyzed by the Chiba University’s soil science laboratory, they provided the data for this study. During the study three type of fertilizer was analyzed which are all organic and the control was a regular chemical fertilizer. For all fertilizers the cost and income of the production were calculated and the profit was weighted with the methane emission what a specific fertilizer produced during the cultivation. In the future if the organic fertilizers are in focus than it is necessary to find a new material what can be competitive with the chemical fertilizers in focus of GHG emission or find an alternative way of the usage of methane in biogas production.
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Fast neutron irradiation long term effect on Ocimum basilicum germination and initial vegetative parameters
23-31Views:231The goal of this study is to assess the long-term (3-year) effects of rapid neutron irradiation using an Americium-Beryllium source on two generations (M0 and M1) of Ocimum basilicum commercial trait. Seeds of each variety and generation were irradiated with 0, 7.5, 20, 40, and 50 Gray in 2018, and then seeded in 2021 – with their corresponding controls – to see how the different irradiation treatments affected germination and early vegetative metrics three years after irradiation. In the first generation, no significant changes between the treatments were seen. Increased irradiation dosage resulted in a significant decrease in germination % in the second generation (M1), but a significant increase in seedling height. The M0 generation seedlings, on the other hand, were substantially shorter than controls. In the M0 generation, the higher dosages (40 and 50 Gray) were deadly, and seedlings were terminated 7–10 days after germination. The irradiation dose of 20 Gray was shown to be the most successful in inducing viable and beneficial alterations for phenotypic characteristics in basil. When compared to control plants, the lower (7.5 and 20 Gray) bombardment generated leaf anomalies such as smaller leaves and internodes, a split apex, and a ribbon-like stem.
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Effect of hail net on the water potential of an apple orchard
109-113Views:508Apple production has seen a decline in yields in recent years, primarily due to ecological factors such as drought, water stress, water scarcity, uneven rainfall distribution, frost damage and hail damage. Ecological factors that are harmful to the plant can be eliminated by human intervention, irrigation or the use of hail net. In our study, we investigated the positive effects of hail net on the development of plant water potential as the vegetation progressed, in non-irrigation area with temperature and humidity. Water potential values were determined using a field osmometer. Confirmed the positive effect of the ice protection net in the apple orchard of Early Gold and Golden Reinders. Our results were supported by statistical analyzes our results.
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Productivity and Nutrient Reaction of Maize Hybrids
78-83Views:337Several factors influence the quantity and stability of maize yield, the most important being the nutrient supply, the hybrid and precipitation. In 2004, during the maize growing season the precipitation was more than the 30 year’s average, with 68.3 mm, but the distribution was unfavorable. The experiment was carried out in Debrecen at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization.
The favorable results reached were due to the rainy season. The average yield varied between 7.78-9.67 tha-1. The DK 440, PR37M34, PR38A24, PR39D81 and PR36R10 of the hybrids reacted to higher fertilizer doses with significant growth yields, the yield (more than 11 tha-1) was the highest for N200, P125, K150 fertilizing. The other hybrids, DKC 5211, Mv Vilma and MV Maraton, gave similar results at the N120+PK fertilizer dose and the ensuing doses depressed the yield. Fertilization was more effective thanks to the precipitation. The fourth and fifth dose increased or decreased maize yield depending on the nutrient reaction of the hybrid. The agro-ecological optimum of NPK fertilization was N120, P75, K90 kgha-1.
During the experiment we tested the moisture loss of five hybrids. The rainy crop year’s effect on the seed moisture content at harvest was higher than in previous years. The seed moisture content hybrids at harvest which have shorter crop years (FAO 300) was 18-19%, and hybrids with longer vegetation periods had more than 20% seed moisture content. DK 440 hybrid had the intensive moisture loss of the five hybrids, at the start of the measurement, the seed moisture content was higher than 40%, and it decreased to 18.6% by harvesting. -
Advancement of a Common Wheat (Triticum aestivum L.) Selection System
8-11Views:198All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect. -
Studying on the frost damage of some peach and nectarine varieties
13-15Views:280To be able to grow stone fruit species, the peach and the nectarine varieties, yield stability is very relevant. However, the climate is suitable in Hungary for growing stone fruits. We have to calculate with damage. Important winter damage and spring frost damage occur in every second year on the Great Pplains. To reveal the frost tolerance of cultivars and to clarify the differences among growing areas is the most important point of peach and nectarine production.
We, made our experiments in the winter of 2005 (January-February), at Pallag, Zsombó, Siófok, Sóskút and Szatymaz. These former places are determinant in peach and nectarine growing in Hungarian respects.
In our experiments we analyzed 10-10 fruit shoot from 82 varieties, and we measured the frost damage and the flower bud density.
The minimum and the maximum frost damage values (%) at the different growing places were 7.6-38.7 at Siófok, 6.5-31.3 at Sóskút, 34.3-100 at Szatymaz, 83.7-99.6 at Pallag and 51.9-99.6 at Zsombó. There were expressive differences between the same cultivars depending on the growing area.
The frost damage of „Suncrest” variety was 7.6% at Siófok, 8.2% at Sóskút but it was 69.8% at Szatymaz. The „Maystar” variety had 17.6% at Siófok, 12.4% at Sóskút and 87.2% frost damage at Szatymaz. In case of „Redhaven” variety the frost damage values were 16.8% at Sóskút, 54.6% at Szatymaz. We collected fruit shoots from three different heights in case of „Cresthaven” variety. In case of 1 meter height the frost damage was 74.6%, at 2 meter height 44.7%, and 25.2% at 3 meter.
We experienced also big differences in flower bud density (flower bud/10 cm) of varieties. The following cultivars had the highest flower bud density (6-7 bud/10 cm): „Suncrest”, „Meystar, „Maria Luisa” and the „Maria Bianca”, „Redhaven”, „Michellini” had the lowest flower bud density (2-3 bud/10 cm).
After our experiments we established that there were very expressive differences among the frost damage of peach and nectarine varieties from different climatic conditions and there are also big variance between the frost damage values of those fruit shoots, what were collected from different heights, but from the same variety. It conspicuous very good among our items, that difference of the flower bud density is very big among the varieties.To be able to grow peach and nectarine successfully, the suitable variety (low frost damage value, high flower bud density) and the suitable growing are is determinant. -
Comparative analysis of sample preparation methods to determine the concentration of arsenic in soil- and plant-samples
167-170Views:476Arsenic contamination of the fields and groundwater is a global problem. Alföld is the most affected area in Hungary. Irrigation witharsenic contaminated water, and crop production on the contaminated soil can cause a food safety problem, because arsenic is easy taken up by the cell of the plant roots. To prevent this, very important to monitoring the arsenic content of soils and plants. Inductively coupled plasma mass spectrometry (ICP-MS) is a fast, easy method to determined the concentration of minerals in the case of plant and soil samples The analytical methods can give reliable, results if the analytical process, including the sample preparation method, is the best. The objective of this study was to compare 3 type of sample preparation method which was dry ashing, wet digestion in open system, and microwave digestion. As a result of our experiement shows the microwave digestion is the appropriate method to determined the arsenic content of soil samples. In the case of plant samples we can use wet digestion in open system or microwave digestion as a samle preparation method.
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Growth and yield patterns of black locust (Robinia pseudoacacia L.) sample trees affected by site conditions: case studies
125-128Views:334The trees removed from the long-term experiment plots are available for the measurements as lying trees. Through the determination of the volume in sections along the stem, the stem form, the stem volume and other factors can be specified. The comparison of the stems of individual trees of first and third yield classes of black locust (Robinia pseudoacacia L.) stands shows that site conditions have a main effect on the yield (mean tree volume). The difference can be as high as 53% at the age of 30 depending on the sites. To determine the growth patterns based on tree volume is rather a new approach in the light of the relevant literature. The obtained results also highlight the importance of choosing the appropriate tree species for a given site.
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Correlations of the global, regional and local factors of the anthropogenic effects on the water reserves of the Earth’s crust
85-92Views:221The decrease in Earth's drinking water resources and the degradation of its quality has become a critical problem. Our planet's total water supply is estimated to be around 2 billion km3s. This is only 1% of Earth's own weight. Of this small amount only a tiny 3% is fresh water, of which 79% is forzen in glaciers and 1% is present as surface waters.The ratio of water stored in soil is around 20%. This is 0.2% of the total water supply. Our study aims to summarize the layered groundwater aquifer systems and its changes which are the results of anthropogenic effects in both global and hungarian respects and also for the region of Debrecen.In particular with regard to the geological and ecological level where irreversible
processes take place. All this is discussed in the context of cause and effect. Pointing out the dangers of excessive deep groundwater extraction and the contamination caused by toxic substances that are the byproducts of modern life. In addition we discuss the Water Directive of the European Union which gives a policy for community action concerning the goal to achive the status of „in good condition” for our waters till 2015. -
Impact of environmental changes resulting from different sowing dates on maize yield
99-104Views:336Three Debrecen maize hybrids of different genotypes (Debreceni 285, Debreceni 377 and Debreceni 382) were examined on chernozem soil in a field experiment. During the two years of the experiment (2009–2010), we wanted to get to know how the examined hybrids reach to different sowing dates and what impact early, optimal and late sowing has on yield.
In 2009, balanced soil and air temperature resulted in steady emergence. However, the low temperature in early April and the cooling down in mid-May 2010 caused a delayed emergence.
The grain moisture content at harvesting and the high yield showed a strong crop year effect. In 2010, yield was much lower (1.664 t ha-1) and grain moisture was significantly higher (34%)than in 2009.
In 2009, early sowing resulted in yield decrease (P<0.05), but it also significantly reduced grain moisture at harvesting (P<0.05). Although late sowing slightly increased yield (not significantly), but grain moisture at harvesting increased by 9.2%. In 2010, optimal sowing date was shown to be the best alternative from the aspect of yield, but there was no significant difference in comparison with early and late sowing. Grain moisture at harvesting greatly increased (13.3%).
The Debreceni 382 maize hybrid reacted to sowing dates flexibly, neither early, nor late sowing affected its yield significantly and the grain moisture at harvesting showed 12% increase in the case of the late sowing date. In 2009, maize hybrids Debreceni 285 and Debreceni 377 reached their highest yield in the case of the sowing date which was shown to be optimal (23rd April), while the different sowing dates had no effect on yield in 2010.
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Effect of supplemental pig milk replacer on piglet weight gain and sow backfat thickness
43-47Views:599Sow milk production is the major factor limiting pig growth to weaning. Although the milk production of the sows incremental many environmental factors affect the actual performances. The supplemental milk replacer can be an appropriate solution to ward off disparities and try to equalize the available milk quantity to the piglets according to their appetite, to enlarge the weight of pigs at weaning, ultimately. The aim of the present study was to investigate the effects of liquid milk supplement on weight development and litter performance of the piglets during the suckling period. Data were collected from 60 farrowings, the weight of 649 piglets in a total were measured at birth, 14 days old and at weaning (28 days), respectively. In the control group (n=319) the piglets were suckled and got prestarter feed from day 10. In the experimental group (n= 330) the piglets had got liquid milk replacer in 10.71% solution, from day 10 after birth together with suckling and prestarter feed, as well. Based on the Duncan's multiple range tests, there were no significant differences in birth weight between the control and experimental group but we found significant differences between the 14 days weight and the weaning weight, subsequently. Based on the CV% of weight the experimental group became more homogenous, in contrary to the weight of the control group at the end of suckling period. The milk supplementation can be an appropriate solution to ward off disparities and to equalize the available milk quantity to the piglets according to their appetite, to enlarge and uniform the weight of pigs at weaning, ultimately.
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Strategic planning in agribusiness
23-27Views:548Agricultural businesses operate in a complex and dynamic environment, with many challenges and opportunities. It is therefore essential for these businesses to have a strategic plan. It is a critical process that helps businesses navigate in a complex and uncertain environment and achieve long-term goals and objectives. In this article, we have conducted a bibliometric analysis of academic journals to investigate the extent to which strategic planning is a researched topic in agribusiness enterprises. We found that there is no concentrated research, with only three keywords appearing in the literature with at least five repetitions. Even of the two repetitions, only 22 were found. The clustering of keywords helps to identify research directions. The results of a survey of 134 enterprises were then presented. The majority of enterprises do not have a long-term plan or even a written vision. We found that SWOT analysis is still the most common planning method among the companies surveyed. It was also found that there is no difference between industries in this respect. Significant differences were only found in relation to company size, with all large companies having a strategic plan and the vast majority of SMEs not. Only 17 out of 132 firms have a plan longer than three years, and 32 do not have any plan at all. Strategic planning can help to address many of the challenges in the agribusiness area, and it is therefore proposed to improve the proportion of firms planning through knowledge transfer.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:604The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:321Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:715Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Botanical Survey of a Seminatural Saline Meadow
38-44Views:191We made a botanical survey of a seminatural meadow situated in the vicinity of Hajdúnánás-Tedej in Hungary, using traditional phytocenological methods. The habitat, based on its soil-, and botanical characteristics, can be classified into the category of saline meadows. One of the landscape ecological values of this area is its mosaic-like structure and, as a consequence, this habitat can be divided into six characteristic associations with blurry borderlines and characteristic overlaps. Because of its rare plant species and species richness this area can be considered to be a valuable „seed bank” and a „stepping stone” in the ecological network among the larger, intensively cultivated fields. The area can be evaluated as moderately recovered, the natural species are dominant, but there are few disturbance tolerant species. Except for the two saline associations, the high grass associations, which make up about two-thirds of the habitat, are cut once a year; therefore, the agricultural utilisation of the area and its prevention against weediness are being assured at the same time.
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Study of effects on quality of different wheat flour fraction
123-129Views:523Wheat is one of the most important cereals in the world and the bread made of its flour belongs to the everyday life of human mankind.
The Hungarian standard relating to the laboratory production of wheat flour (MSZ 6367/9-1989) does not mention the type of laboratory mill used for milling, and it only builds up some general criteria, such as: the laboratory mill should be provided with four differently nicked barrels, a sieve with appropriate hole sizes, and also with the separated collections of the pilot flour and the bran. Our study was started at this point and the answers for the following questions were aimed to be found: do the flour patterns studied and produced with different grinding and sieving
techniques, widely used in laboratory mills of the same wheat pattern show any alterations after the impact of the formula production as regards chemical constitutions and reologic parameters. Various flours and whole grains of the wheat patterns sieved with different particle sizes were studied in this experiment. In producing this pattern two different mill types of FQC 109 and CHOPIN CD 1 as well as two different grinder types such as PERTEN 3100 and type of RETSCH 200 were applied. There were 3 different corn sizes of 160; 250; 800 μms used in the partition of the fractions. To study the differences the following measurements were conducted: dry matter, ash, protein content, wet gluten content, gluten index, gluten expansiveness, farinographic value, falling number and amilographic rate.
The results this research confirm that the quality of wheat flour can be modified by different methods of pattern production. In all cases the differences can be explained by the flour-bran ratio, and in some of the cases the higher germ content of the fractions also played a role. The results show differences between the various types of mills and grinders, too.