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Seed protectant potential of Mitracarpus villosus and Phyllanthus fraternus extracts on germinative capability of stored food grains
7-10.Views:497Seed storage is an essential post-harvest operation that decides the success of seeds viability and germination in next generation. The study explored Mitracarpus villosus and Phyllanthus fraternus extracts as bio-insecticides seed treatments on stored wheat and green gram seeds viability and germination. M. villosus and P. fraternus plants powder were sequentially extracted with solvents of increasing polarity (Petroleum ether, hexane, ethyl acetate, acetone, chloroform and methanol), concentrated and tested for insecticidal activity by fumigant toxicity. The extract which showed maximum activity, was selected for seed viability and germination test. Five replications each were made for the treatment and the control. The plant extracts strengthen the non-phytotoxic nature of plant products against seed viability and germination. Seeds treated with extracts (50-400 μg seed-1) did not lose their viability as it resulted in successful and normal germination within the range of 90 – 97.67% irrespective of the extracts concentration. While, seeds in control recorded 95.55 and 100% germination which were not significantly different (P>0.05) compared to the treated seeds. Based on findings from the study, potential exploitation of M. villosus and P. fraternus as food grains protectant in insect pest management strategies is recommended for the resource poor farmers. However, further investigations are suggested on biosafety and effects of the extracts on the organoleptic contents of the grains prior to consumption.
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Germination, seedling growth and dry matter accumulation of Cola nitida in the nursery as affected by seed biotype colour and storage duration in dodecahedron pyramidal device
67-76Views:87Long dormancy period and non-uniformity growth patterns of Cola nitida pose challenges for kola farmers, discouraging them from establishing kola plantations. Experiment was conducted at Federal University of Agriculture, Abeokuta, Ogun State, Nigeria to study the effects of seed colour and curing duration on germination, growth and dry matter yield of C. nitida. Experiment was 3 × 4 factorial, laid out in Completely Randomized Design. Treatments comprised seed colour (red, pink & white) and curing duration (12, 8, 4, and 0 weeks). The experiment had 12 treatments; each replicated three times. Data collected on germination, growth and dry matter were subjected to analysis of variance and means were separated using Duncan’s Multiple Range Test (P ≤ 0.05). Germination commenced at 4 WAS, on white, pink and red kola seeds stored for 8, 4 and 6 weeks with 10%, 6.66% and 3.33% respectively. At 8 WAS, white kola seeds stored for 12, 4 and 8 weeks had 93.3, 86.7 and 83.3% germination respectively. At 12 WAS, germination was completed for white kola 12-week stored in Dodecahedron Pyramidal Device (100%), while others range between 60.0 and 96.7%. White, pink and red kola seeds stored for 8, 4 and 12 weeks produced vigorous seedlings that were taller with more leaves than those of an un-stored seed at 18 and 20 WAS. In conclusion, white, pink and red kola (C. nitida) seed stored for 4, 8 and 12 weeks inside Dodecahedron Pyramidal Device resulted in rapid germination and improved morphological growth performance.
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In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants
15-20.Views:800Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.
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Phytobiome management in horticultural systems: beyond the rhizosphere toward whole-plant microbiome optimization
85-101.Views:82The plant microbiome is not confined to the soil. Across every anatomical compartment, the rhizosphere, phyllosphere, endosphere, anthosphere, spermosphere, and carposphere, structurally distinct and functionally specialized microbial communities orchestrate processes fundamental to plant health, productivity, and resilience. Yet horticultural science has remained disproportionately anchored to the rhizosphere, leaving the vast microbial landscape inhabiting aerial, floral, seed, and fruit tissues largely unexplored and unmanaged. This blind spot carries profound consequences at a time when global horticultural production confronts an unprecedented convergence of pressures: accelerating climate instability, stringent restrictions on synthetic agrochemicals, mounting soil degradation, and escalating consumer demand for sustainably produced, chemical-free, premium-quality produce. The biological potential embedded within the whole-plant phytobiome to address these intersecting crises remains critically underutilized. A fundamental barrier to progress is the absence of a unifying scientific framework. Existing reviews address plant-associated microbiomes in disciplinary silos, focusing narrowly on rhizosphere bacteria, individual crop species, or single microbial kingdoms, without synthesizing the full cross-compartment, cross-kingdom phytobiome in the horticultural context. No comprehensive framework has yet integrated microbial community dynamics spanning bacteria, fungi, archaea, and viruses across fruit, vegetable, and ornamental crops within a single, coherent, and practically applicable model. This review addresses that gap directly. Through critical synthesis of compartment-resolved phytobiome research across major horticultural systems, we characterize the taxonomic composition, ecological assembly drivers, and agronomic functional roles of microbial communities inhabiting each plant compartment. We demonstrate that phyllosphere microbiomes confer photoprotection and pathogen exclusion; endophytic communities directly modulate secondary metabolite profiles and systemic immunity; anthosphere microbiomes influence pollinator attraction and fruit set; spermosphere communities determine seedling establishment success through vertical microbial inheritance; and carposphere microbiomes govern post-harvest storability and food safety outcomes. We further establish how host genotype, crop developmental stage, management-induced dysbiosis, and climate-driven perturbations collectively shape phytobiome assembly and functional integrity across compartments. Building on this synthesis, proposing to introduce the Whole-Plant Phytobiome Optimization (WPPO) framework, the first integrative, three-tier conceptual model designed specifically for horticultural systems. WPPO encompasses whole-plant phytobiome profiling using multi-omics platforms, identification of functional microbial modules linked to target agronomic traits, and precision compartment-targeted intervention through synthetic microbial communities (SynComs), encapsulated biostimulants, and digitally guided delivery systems integrated with IoT sensor networks and machine learning decision-support tools. Applied across the full crop life cycle, from spermosphere conditioning at seed priming to carposphere biopreservation at post-harvest, WPPO offers a scalable, evidence-based, and technologically integrated roadmap toward substantially reduced agrochemical dependence, enhanced crop resilience, superior produce quality, and the deployment of ecologically precise next-generation biocontrol and biostimulant strategies.
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Influence of time of planting on yam performance and pest infestation in humid rainforest of southwestern Nigeria
77-84.Views:19Yams (Dioscorea spp.) are annual or perennial tuber-bearing and climbing plants that belong to the family Dioscoreaceae. Only six principal species are grown for human consumption, while several others are produced for medicinal purposes. This study was designed to determine the influence of time of planting on yam performance and pest infestation in humid rainforest of southwestern Nigeria. The experiment was conducted in Randomized Complete Block Design (RCBD) with three treatments replicated five times. The planting materials are dipped into wood ash and allowed to dry up. The number of yam setts required for this work is 4 pieces, each of white yam sett, seed yam, and 4 pieces of water yam setts for the different planting times. The planting is done in five (5) phases. In each phase 12 heaps are planted, 4 white yams (seed yam), 4 yam setts (white yam) and 4 water yam setts. No significant difference was observed in 50% sprouting both in treatments and replication. There is significant difference in yield both in treatment and block. Yam planted in April (R5) has the highest yield with 3.7 kg, followed by those planted in ending March (R4) with 2.83 kg, followed by those planted in mid-February (R1) with 1.43 kg, then those planted in February ending (R2) with 1.2 kg and those planted in mid-March (R3) with 1.17 kg respectively. The highest level of destruction was witnessed in white seed yam planted in Mid-March and Mid-April as well as white yam sett planted in Mid-February. The lowest level of destruction was found in water yam sett planted in February ending. Shifting planting date can reduce larval survival and pest populations in yam tuber.
Keywords: Dioscorea spp., pest infestation, destruction, sprouting, yam performance.
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Interaction impact of drought stress, nutrient-deficient water, and seed-borne pathogen (Alternaria alternata) on germination and vigor of two tomato varietiesination and vigor of two tomato varieties
48-53.Views:342Tomato (Solanum lycopersicum) is considered one of the leading vegetable plants in the world. This study evaluated the germination and vigor capabilities of ‘Marmande’ and ‘Kecskeméti Jubileum’ varieties under different conditions, including drought stress, nutrient-deficient water, and the effect of seed-borne disease caused by Alternaria alternata when prime and non-prime with salicylic acid. The experiment was conducted in the laboratory in 2023 at Agricultural and Food Sciences and Environmental Management Faculty of University of Debrecen. Results indicated that the ‘Kecskeméti Jubileum’ variety exhibited a superior strength to ‘Marmande’ in the seedling’s dry weight, seedling growth rate, and vigor index under nutrient-deficient water. Nevertheless, when applying levels of drought, the ‘Marmande’ variety had a higher viability rate of 62.5% in comparison with another variety, and the germination rate of the two tomato varieties is at 85.5% in 5% concentration but decreased progressively when exposed to a higher drought concentration of 10%. When using 3 ml of salicylic acid during germination stages, the seedling vigor index of ‘Marmande’ shows a greater index at 165 compared to ‘Kecskeméti Jubileum’, just 108 under the infection of Alternaria alternata. The results of the examination of drought stress, and the effect of Alternaria alternata, one cause of seed-borne pathogens, showed that the percentage germination and vigor ability of the ‘Marmande’ variety performed better than ‘Kecskeméti Jubileum’ under the same conditions.
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Propagation from root cuttings for black locust (Robinia pseudoacacia L.) improvement in Hungary: a review
39-41.Views:606Black locust (Robinia pseudoacacia L.) is a valuable stand-forming tree species introduced to Europe approximately 400 years ago from North America. Today it is widely planted throughout the world, first of all for wood production. In Hungary, where black locust has great importance in the forest management, it is mainly propagated by seeds. But since the seed-raised plants present a great genetic variation, this type of propagation can not be used for Robinia’s improved cultivars. In the Hungarian black locust clonal forestry, propagation from root cuttings can be used for reproduction of superior individuals or cultivars in large quantities. However, this method demands more care than raising seedlings from seeds and can be applied with success in well-equipped nurseries.
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Generative propagation of Robinia x ambigua POIR. – Pink locust
41-42.Views:551The genus Robinia is a small group of about 10 species of trees and shrubs indigenous only to NorthAmerica. Two species are endemic to Mexico, one being confined to south-western part of the country, while the rest are endemic to the south-eastern part of USA. Of the most important species and varieties of genus Robinia, Robinia x ambigua Poir.(Robinia viscosa x R. pseudo-acacia)-pink locust can be considered as the most significant one for bee-forage and decorative planting. In this paper a generative propagation method is presented for pink locust.
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Black locust (Robinia pseudoacacia L.) selection programmes in Hungary: a short review
31-34.Views:563Black locust (Robinia pseudoacacia L.) was the first forest tree species introduced from North America to Europe, at the beginning of the 17th century. Its unprecedented fast spread is due to its high-grade adaptability, drought-tolerance, abundant and frequent seed crop, excellent sprouting ability, fast growth and relatively high timber yield. Other advantages are, that it has scarcely any fungi or insect pests. This review is a short summary on black locust improvement in Hungary, giving guidance for specialists who are interested in black locust management.
