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Some flower characters, variety features and environmental effects affecting bee pollination of pear (Pyrus communis L.): a review of the results of latest research
59-66.Views:344Intensive research has been implemented on different aspects of the bee pollination of pear in Hungary in the past decade, extending to the following topics: the effect of the opening sequence of the flowers within the inflorescence of pear cultivars on the effectiveness of bee pollination; the nectar production of flowers of pear cultivars; intensity of honeybee visitation and their foraging behaviour at the flowers of pear cultivars; flower constancy of honeybees to pear plantations; competition by weeds in bloom for bee pollination in flowering pear orchards; and some aspects to the bee pollination strategies of pear plantations (the effect of the limitation of bee pollination period on the set and the yield, possible compensation of frost damage of flowers by bee pollination in pear orchards, the optimal size of single cultivar block in pear plantations). The results of these studies are reviewed in this paper and some important aspects that need further studies are outlined.
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The effects of spinosad insecticide to adults of Apis mellifera, Megachile rotundata and Nomia melanderi (Hymenoptera: Apidae)
93-97.Views:392The toxicity of spinosad to adult female bees tended to be least to the honey bee (Apis mellifera L.) (LD50 = 0.078 pg/bee), intermediate to the alkali bee (Nomia melanderi) (0.065 pg/bee), and greatest to the alfalfa leafcutter bee (Megachile rotundata (F.)) (0.058 pg/bee), both in topical drop tests and in tests involving spinosad residues on alfalfa (Medicago sativa) foliage. For the calculated LD50 pg/g, the honey bee (LD50 = 0.612 pg/g) was the most susceptible followed by the alkali bee (0.773 pg/g) and the leafcutter bee (1.908 pg/g). The honey bee oral LD50 was 0.063 pg/bee and the calculated LD50 0.492 pg/g. Adding an adjuvant to spinosad sprays did not change the toxicity of spinosad to bees in residue bioassay studies. Spinosad at as high as 500 ppm in feeders containing a sucrose/honey syrup caused no significant reduction in honey bee visitation or total syrup consumed.
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Improvement of the grapevine variety ‘Kadarka’ by the selection of new clones
57-63.Views:302Red wine culture in Hungary was developed due to the grapevine variety ‘Kadarka’, originated from the Balkans, from the 16th and 17th centuries. ‘Kadarka’ was the first grapevine variety cultivated on the largest area in Hungary until the 1950’s, but its production area has been reduced significantly in the past decades. In the period of Hungarian grapevine reconstruction (from the 1960’s), the selection of the grapevine varieties and clones was determined by the system of cultivation (large growing space, large load and mechanized technology) that was based on the aspects of quantity. It did not promote the majority of the autochton and regional grapevine varieties to remain competitive. ‘Kadarka’ is a grapevine variety having an extraordinary variability in forms; however, a considerable part of its old stocks has disappeared. Nowadays, only two of its high yield clones are cultivated. ‘Kadarka’ becomes more differentiated from other, red wine producing, grapevine varieties, more preferred and newly requested by the fine aroma, spice, unique harmony of its wine less rich in tannin. The new market and environmental challenges in the field of wine production necessitate the preservation and improvement of the biological basis. In 2001 the Institute of Viticulture and Enology Pécs analyzed an old stock having a variability in forms in order to preserve the development and variability of the biological basis of ‘Kadarka’. Clones of great biological value were selected from it, with which the quality and yield security of ‘Kadarka’ can be increased. In 2010 and in 2012, six clones were submitted for state approval.
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Vegetative Indicators of sour cherry cultivars and resistant clons
55-58.Views:343Sour cherry is a hungaricum. Hungary has several excellent and self fertile sour cherry cultivars. Hungarian sour cherries have a name for fruit quality and they are suitable for fresh consumption. Today breeding is focused not only on fruit quality, but also on disease resistance. The new, investigated cultivars have these characteristics too. But growth characteristics are also very important because they contribute to successful production. The vegetative and generative properties of the cultivars are different. If a cultivar is very productive, the ignorance of cultivar-specific pruning may result in the decrease in tree condition and fruit quality. The properties of producing fruiting wood greatly determine the renewal capabilities of cultivars. Knowing these properties is essential in case of sour cherries, the fruit species that requires a lot of sunlight and tends to produce bare wood. The research of candidate cultivars until now focused on fruit quality. Our aim was to investigate the vegetative characteristics of the resistant ‘bosnyák’ cultivar group in detail, defining the growth characteristics of these cultivars.
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Summer pruning of sweet cherry trees and an inquiry of winter frost damages
41-44.Views:9922One of the most demanded research projects is the intensification of fruit production. The use of dwarfing stocks is a moderate solution as their effect is scarcely satisfactory. Climatic conditions of Hungary are continental in Eastern Europe, where Atlantic and Mediterranean effects are interacting with the continentals in a kind of basin with characters of its own. Capricious meteorological episodes are often disturbing the security of development and fruiting of trees:
• winter frosts are damaging the cambium and fruiting structures of trees
• late spring frosts destroy cambium and flowers
• early autumn frosts hurt the leaves
• excessive precipitation impairs the growing fruits
• drought periods during the summer caused water stress disturbing water husbandry.Vigorous stocks still prevail in the practice, and they ought to withstand challenges of weather hazards. The strong vigour of plants delaysthe process of senescence and the tendency of getting bald, and regeneration of plants is a sign of vitality. In present research, the trees have been trained on vigorous Prunus mahaleb stocks. Summer pruning was one of the important tools of intensive growing techniques. They were compared with traditional techniques and with plastic foil protected trees observing the vegetative as well as generative growth of them.
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Changing of inert content values of apricot varieties
39-41.Views:477The consumers have been exigent increasingly about excellent quality. The speci fie flavour and fragrance of apricot evolve during ripening. We need to be acquainted with changing of inert content during ripening to the choosing of optimal harvest time. The Regional University Knowled ge Centre -Research and Development in Foodstuff Chain project had presented an opportun ity to study the inner content of apricot. Our aim was to study evaluation of health care attributes of apricot fruit. We investigated different ripeness samples of four apricot varieties ('Mandulakajszi', 'Pann 6nia', 'Gonci magyar kajszi'. 'Harcot') in our study of 2007-2009. Samples were taken the orchard of Sóskútfruct Ltd. Hungary. We had measured the water soluble antioxidant capacity, polyphenol-, f3-carotene content and the dissimilar sugar and acid-fractions during ripening. We had experienced significant different in inert content between varieties.
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The effect of climatic anomalies on the nutrient supply of fruit plantations (Minireview)
111-116.Views:12784Climatic conditions play an important role in agricultural production. It has a profound influence on the growth, development and yields of a crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water stresses. Nowadays, we have to know the dark side of the weather events because it is causing more and more problems and significant hazards to many horticultural regions in Hungary. The aim of this study is to explore the problems of nutrient uptake following climatic anomalies and response. These problems are: (i) water supply problems (water-stress); (ii) drought and frost as temperature strees. Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
- Nutrient demand of trees can be supplied only under even worse conditions.
- The most effective weapon against damage of climatic anomalies is preventative action.
- When developing a fruit orchard, three factors should be taken into consideration: "Location, Location, Location".
- Moreover, proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of climatic anomalies.
- Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
- Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar fertilization is recommended, which adjusted to phonological phases of trees.
Another solving is groundcover of soil means a potential opportunity to temper or even avoid climatic anomalies.
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Effects of tuberization conditions on the microtuber yield and on the proportion of microtuber tissues
91-96.Views:232The production facilities of large-sized microtubers in three potato varieties (cv. Desiree, BorO, Gfilbaba) and the effects of the applied tuberization conditions on the proportion of microtuber tissues, especially on the perimedullary region were investigated in present work. In vitro tuberization was induced on explants with 2 or 5 nodes layered on MS medium supplemented with 8% sucrose. Induced cultures were exposed to short days (8 h) for 2 weeks, then to total darkness for further 11 weeks. For volume calculations of different tissue regions, the formula for ellipsoids (V=4/37c1/8/w2) was used. The number of large-size tubers (> 8 mm, up to 16 mm) reached 53%, 59% and 44% in cvs. Desiree, Giilbaba and Bore, respectively, which indicate that the size of microtubers could be increased by appropriate sucrose support and explant type. Microtubers produced on hormone-free medium have well-developed perimedullary region, and its volume rate seemed to be important in the final size of tubers. The increase in the rate of volume of the perimedulla was connected to the increase of tuber size until tubers reached 12 mm diameter. In microtubers larger than 12 mm in diameter, the volume rate of the pith was increased.
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Effects of crop load on tree water use in apple (Malus x domestica Borkh.)
29-31.Views:290Sap flow rate measurements were carried out during two consecutive ('on' and 'off') years in an apple orchard cv. 'Florina' M.26 to analyse the effects of various crop load on tree water use. Sap flow rate was measured by thermal dissipation method in trunks of nine trees from June to the harvest. Crop load was between 0.2-9.0 fruits • cm -2 of trunk cross section area (equal to 0.5-35.0 fruits•m-2 of leaf area), trunk diameter varied between 4.7-8.7 cm.
Total leaf area was estimated by leaf counting or using shoot girth and leaf area relationship. In both years, fruit growth rate was determined by measuring diameter of 280 marked fruits with 7 days frequency. Fruit volume calculated as a function of fruit diameter based on a previously determined relationship.
Total leaf area of trees ranged from 4.4 to 19.5 m2 and it was closely related to trunk cross section area. At high crop load the fruit growth rate peaked in August with 0.51.tree-l.day-1 and the leaf area specific total fruit volume reached 61 m2 before the harvest.
There was a linear relationship between total leaf area and daily water use, while the leaf area specific water use was influenced by crop load rate. The relationship was described by piecewise linear regression with the breakpoint at crop load rate of 12 fruits•m2 of leaf area. At low crop load the slope of the fitted regression line was less than at high crop load rate.
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Organoleptic evaluation of pear varieties grown at different sites
33-37.Views:970It was stated as a summary that a system of testing pear varieties on scale of 100 degrees, weighing 11 properties, gives a comprehensive information on qualities of pear varieties. The points of the form to be filled out, distinguishes the appreciation of accessory flavours and tastes from the main properties. Under the title of taste, sweet and acid taste are evaluated separately. The presently used system is a step forward as the composed characters do not influence the judgement of each other. In the representation of results, the 100 degree system was more efficacious than the usual 5-point system because there was no need of using fractions, and the graphic profile is more expressive, so the commodity is better visualised. The assembly of the jury was divided into two groups as below and above 35 year old persons. The two groups judged differently the acidity, sweetness and the thickness of the fruit peel. It was suggested that the choice of the produce should consider the differences in taste and give up the idea of being equally favourable for both groups. The samples of different growing sites revealed that the best quality is produced at Nagykanizsa, and this capacity would be able to supply the market also in the large super-markets and chain of department stores in Hungary.
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Pollen viability of ‘Besztercei plum' clones depending on the effect of the year
115-121.Views:537The percentage of dark staining pollen grains was higher in spring of 1996 than in the previous year. Data in 1998 resemble those of 1995, concerning the large amount of medium staining pollen grains in the majority of clones. Some clones produced excellent quality pollen also in the third year. whereas there were significant differences in other clones in various years.
The warmer February-March period in 1995 induced an early blooming, and frost affected the orchard not only in winter months, but also immediately before and during blooming. Thus, frost was the possible cause of weaker quality pollen this year. In 1996 warming began a bit late, but it was not broken by drastic falls in temperature, except for the middle of April, when a smaller frost affected the orchard. It is likely that this frost did not influence pollen quality of `Besztercei' and 'Early Besztercei' plum clones significantly. In 1998 warming was continuous and steady, the orchard was not affected by frost immediately before blooming. In March, however, there was frost almost every day, according to daily minimum temperatures.
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Effect of foliar spraying with algae suspension on leaf and fruit quality parameters of apple varieties
35-38.Views:1292Foliar nutrition experiment was made to investigate the effect of algae products on fruit quality of apples (Malus domestica Borkh.). The study was conducted in 2011 at Siófok inWest-Hungary on cv. ‘Jonagored’ and ‘Idared’grafted on M9 rootstock. In our trial leaf diagnostic and fruit quality measurements were made to study the effectiveness of applied products. Relative leaf chlorophyll content was determined by a portable chlorophyll meter. Fruit weight and shape index were measured. Fruit inner qualifying parameters like titratable acid content and Brix value were also determined. It can be stated that the used products had traceable effect on investigated fruit parameters. Although, leaf N content was not effected by treatments significantly, relative leaf chlorophyll content significantly increased by both treatments. Used products significantly increased the fruit weight and shape index. Applied treatments significantly decreased the pH of pulp. Treatments increased the acid contents (fumaric, citric, malic) of apples and decreased the amount of monosaccharides. It means that the applied treatments pushed out the ratio of acid/sugar.
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Enrichment trials with straw substrate to produce Agaricus bisporus (Lge., Sing.)
7-9.Views:226Everywhere in the world button mushroom is cultivated on heat-treated horse manure or on a compost mixture of straw, horse manure and chicken manure. In the ZKI trials continued on straw which seemed to be a simple and cheap substrate. To improve yield the N-content of the straw was increased by mixing it with wheat bran, alfalfa flour and ProMycel. Enriching agents were added to the dry heat-treated straw (100 "C) at 1, 2, 3 weight per cent. All the 3 agents increased yield as related to control. The highest yield was obtained with ProMycel.
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Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.
65-71.Views:270The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.
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Optimizing disinfection protocols for yam explant regeneration in plant tissue culture
66-72.Views:648Yam (Dioscorea species), does not produce commercially viable seeds, and asexual propagation is faced with challenges resulting from carried-over infections from previous generations. Contamination is a prevalent problem in Plant Tissue Culture (PTC), making the development of cost-effective and efficient disinfection protocols crucial for successful PTC. This study aimed to evaluate the effectiveness of yam explant disinfection protocols using various immersion timings, disinfectant concentrations (including ethanol (C2H5OH), sodium hypochlorite (NaOCl), and hydrogen peroxide (H2O2)), and in single or combined disinfectants application. Twenty treatment combinations and one control were assessed on yam vines for Disinfection Efficiency (DE%), Negative Disinfection Effect (NDE%), and the regeneration of shoots and roots (SN & RN) from the culture after 21 days. The study showed that varying immersion times did not significantly impact the evaluated parameters. However, different concentrations of disinfectants resulted in diverse NDE responses. Surprisingly, higher concentrations of NaOCl led to reduced NDE, whereas lower concentrations increased NDE. On the contrary, higher concentrations of H2O2 increased NDE, while lower concentrations decreased it. Shoot and root regeneration rates were also significantly impacted by the choice of disinfection protocol. The research concluded that dual disinfection protocol, specifically 70% ethanol for 7 minutes followed by NaOCl, was most effective for eliminating surface-borne contaminants and achieving successful in vitro propagation of yam plantlets. This method offers a cost-effective solution for establishing microbe-free tissue culture yam plantlets and provides a basis for future research on other Dioscorea plants.
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Casing-material experiments with Pleurotus eryngii
33-36.Views:538Our research led to the gathering some relevant information about the growing technology of the Pleurotus eryngii. In some European countries this mushroom is quiet favourable. The detail of the growing technology has not been determined in detail yet. These partial results led us to carry on with the research towards a development of covering technology. With some new series of experiments and with the use of some new covering mixtures and their application of different thickness we are hoping to clarify the details of the technology.
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Xanthomonas resistance in Hungarian 4 spice pepper varieties
73-77.Views:299With a view to further enhance the reputation of Hungarian spice pepper it was necessary to improve resistance to the bacterium Xanthomonas campestris pv.vesicatoria, the most dangerous pathogen of pepper varieties. From among the familiar resistance genes in Hungary only the gene Bs-2 could provide sufficient protection against the aggressiveness spectrum of the bacterium species X.c.pv. vesicatoria. The first results of the resistance breeding are the spice pepper varieties Kaldom and Kalorez. In addition to the Bs-2 gene attempts are also being made at building in a gds gene into pepper, a gene creating a general defense system, a different strategy towards Xanthomonas campestris pv. vesicatoria.
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Scheduling of ornamental plant production
76-86.Views:282The production of ornamental plants represents an important branch of our horticulture. The growing area is relatively small (round 2800 ha), the production value, however, a rather large. Notwithstanding, its financial balance is inactive because the demand surpasses the supply copiously.
The most popular plants are as follows: carnation, gerbera, rose, bulbous flowers, chrysanthemum, other cutflowers, cutgreens and Gypsophyla — their total production value makes up to Ft 8-9 billion. The area of ornamental nurseries — about 800-900 ha — is to be found mostly on the western part of our country.
Our accession to the EU will have undoubtedly an impact on our ornamental plant production. We must take into account, that in greenhouse production the specialization extends all over the world, field production over a limited region. Our chances will not deteriorate by joining the EU. The buyer — chiefly because of ecological purposes — will prefer the domestic product to the foreign one. By the way, our products will be competitive, as far as quality or price is concerned, with those of western Europe. Last but not least, we may hope a greater saleability of home-bred, special varieties and cultivars, the so-called "hungaricums", both in the domestic as well as export markets. It is anticipated that we will have a good turnover with potted ornamental plants and flower seedlings. Our nursery products will become marketable too.
To exploit the opportunity, of course, the necessary conditions are to be created. First of all, we need development in research, with special regard to breeding, at the same time in education, in extension service, in the training of experts, on a high level. Some tasks can be solved, no doubt by improvement of the organisation within the branch. At the same time the state subsidy is indispensable in order to promote both the technical and the research activities. State subsidy is also necessary to build up more advantageous conditions of sales like in some foreign countries (e.g. the Netherlands).
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Agromorphological and nutritional quality profiles of fluted pumpkin (Telfairia occidentalis Hook F.) as influenced by cultivar, growing medium and soil amendment source
53-59.Views:305Fluted pumpkin (Telfairia occidentalis Hook F.) is popular as food and feed around the world. Sixteen treatments were developed from factorial combinations of three factors: cultivar (ugu elu and ugu ala), growing medium (garden soil (GS) and white sand (WS)), and soil amendment source (poultry manure, NPK, supergro and no amendment). A pot experiment was conducted to investigate the agromorphological and nutritional traits of fluted pumpkin obtained from the treatments. Fresh leaves were analyzed for crude protein, crude fibre, crude lipid, total ash, phytate and nitrate concentrations. Data were subjected to analysis of variance and principal component analysis. Mean plots were used to explain the effects of the three factors and profiling was done using the GYT biplot. There were significant (p≤0.05/0.01) mean squares for measured traits, suggesting the possibility of selection among the treatments. Plants in GS consistently out-performed those in WS for shoot weight, leaf length, and number of leaves per plant possibly due to greater availability of nutrients in the GS. Inconsistent patterns observed in the proximate concentrations of pumpkin from the 16 treatments showed the role of interaction among the three factors. Principal component analysis identified some traits as contributors to differences among the treatments which can be basis of selection. Treatments 2, 4, 6, 8, 10, 11, 12, 13, 14, 15, and 16 might be useful to improve vegetative yield while 1, 3, 5, 7, and 9 could improve nutritional values of the fluted pumpkin.
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Valuable offsprings of Seyve-Villard 12375
75-77.Views:352Powdery mildew, Phylloxera and downy mildew epidemics devastated large areas of vineyards in Hungary in the 1800s. To establish new plantages resistant so called "direct producers" were imported from America (direct means "without grafting"). Direct producers have secure yield but their quality is inferior. After World War II, an organized breeding programme was started to replace them and parallel to this the law prohibited their production. As a resistance gene source, the E.2 clone (S. V. 12375 E.2) of Seyve-Villard 12375, Franco-American hybrid selected in Eger was used by Hungarian breeders. Several valuable hybrid families have originated from crosses of this variety hybrid.
In the Research Institute for Viticulture and Enology at Kecskemét, two valuable table grape hybrids were registered under the names of R.65=Esther and R.78=Fanny coming from 2 hybrid families of the parent S. V. 12375 E.2. Both varieties may be cultivated "environmentally".
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:396The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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The role of black locust (Robinia pseudoacacia L.) in establishment of short-rotation energy plantations in Hungary
41-44.Views:492Establishment of short-rotation energy plantations for fuel production has been of international interest for many years. Energy plantation experiments in Hungary have been conducted for a longer time. In the country black locust ( Robinia ps eudoacacia L.) is one of the most important stand-forming tree species, covering approximately 23% of the forested land (410 000 ha) and providing about 19% of the annual timber output of the country. This fast growing species seems to be suitable for energy plantations as well. So, in Helvecia (Central Hungary, sand-soil region) two energy plantation s were established u sing common black locust and its cultivars improved in Hungary. The spacing variations of the common black locust were: l.5x0.3 m, I .5x0.5 m and l.5x 1.0 m. At the age of 5 the closest spacing ( 1.5x0.3m) produced the greatest annual increment in oven-dry weight (6.5 t ha·1 yr- 1). In the trial with black locust cultivars planted in spacing of 1.5xl.0m, at the age of 7 the highest annual increment in oven-dry mass was produced by the cultivar ' Ulloi' (9.7 t ha-1 yr- 1) followed by the common black locust (8.4 t ha-1 yr- 1) and the cultivar 'J tiszkiseri (1.6 t ha·1 yr- 1). The trials have verified that in temperate climate the increment in oven dry dendromass of black locust energy plantation s has ranged from 6 to 12 t ha·1 yr·1. On the basis of the trials' evaluation the quantity of dendromass mostly depends on site quality, species and cultivars, as well as on the initial spacing (plants per hectare).
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Identification of ripening-related genes in strawberry fruit by cDNA-AFLP
33-41.Views:298An RNA fingerprinting study of strawberry receptacle and achene tissue was performed to identify candidate genes involved in fruit ripening. Quantitative cDNA-AFLP was used to detect differential gene expression in green, white, pink and red stages of fruit ripening. Based on hierarchical average linkage clustering the differentially expressed genes formed three major groups, genes expressed only in green receptacle, genes expressed mainly in white, pink and red receptacle, and in achene. 130 transcript-derived fragments (TDFs) were isolated and sequenced. Most TDFs did not show any homology to sequences with known functions, others were homologous to genes involved in oxidative stress response, signal transduction, regulation of development and cell-wall metabolism. Novel genes, so far not associated with strawberry ripening and ripening in general, were identified, such as genes encoding a bHLH protein, putative nitrilase-related protein, putative HD-zip protein. The differential pattern of gene expression draws the attention to the significance of ripening induced-or repressed promoters in strawberry fruit, whose isolation and characterization can be useful tool for functional genomics. For this purpose nine cDNA-AFLP fragments related either to ontogeny or senescing were completed with 5'UTR aiming at more precise annotation and future promoter isolation. Although tens of potentially important transcriptome changes were identified, the function of many ripening induced genes remain unknown.
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Cytokinins affect the stomatal conductance and CO2 exchange of in vitro apple leaves
25-28.Views:488Effects of different cytokinin supplies including two types of aromatic cytokinins, such as benzyl-adenine, and 3-hydroxybenzyladenine applied at two different concentrations (2.0 and 6.0 μM) were studied on water and gas exchange parameters in in vitro apple leaves of ‘Royal Gala’ and ‘Freedom’ scions after 3 weeks of culture. Cytokinin supply affected the stomatal conductance of water vapour, transpiration rate and the sub-stomatal CO2 concentration of leaves. Effect of cytokinin depended on its applied type and concentration, moreover on the apple scion. According to the results, the rate of CO2 exchange itself is not usable for characterization of function of photosynthetic apparatus of in vitro leaves. However, measurements of stomatal conductance of water vapour and transpiration rate seemed to be good indicators for stomatal behaviour of in vitro apple leaves.
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Flower density and winter damage of apricot and peach varieties
53-56.Views:746Hungary is located on the northern boundary of economical apricot and peach production. The present assortment of varieties and the actual, not adequately selected growing sites cause a permanent risk of winter and spring frosts in their cultivation. The field observations are performed at Debrecen, the Experiment Station Pallag, on 20 apricot and 21 peach varieties. The flower density among the varieties attained 3-4-fold differences. Three categories have been suggested for both species. The density was inferior in Hungary established varieties compared with the new varieties of foreign origin. The minimum temperatures of January 9, 2009 was –17,6 °C , and of December 21, 2009 also –17,6 °C. In some varieties the damage of buds attained 100%. For estimating the yield security, we need to consider also the flower density and the frost damage together. For a mediocre yield, we need a flower density in both species of at least 0.2 living bud/cm. The results confirm the statement that in Hungary, the revision of growing sites is indispensable in order to develop a profitable and competitive apricot and peach growing industry.
