In broiler chickens, the intensive selection for growth rate, feed efficiency, body composition (breast muscle weight) traits in the last decades was successful. To improve economically important characteristics, it is possible to use molecular markers associated with meat production traits. The aim of this study was to examine genotype polymor...phisms in ROSS 308 broilers for thyroid hormone responsive Spot14α, insulinlike growth factor 1 (IGF1), IGF-binding protein 2 (IGFBP2), somatostatin (SST) and prolactin (PRL) genes. A further goal of this investigation was to study the relationship between the polymorphisms and phenotypic characteristics.
In the investigated broiler population, the frequency for CC homozygous genotype was 0.77 in Spot14α (AY568628), AA homozygous genotype was 0.80 in IGF1 (M74176), GG homozygous genotype was 0.85 in IGFBP2 (U15086), DD homozygous genotype was 0.60 in PRL (FJ663023 or FJ434669). Only the AA homozygous genotype was found in SST (X60191). Chickens with AC genotype in Spot14α, and with GG genotype in IGFBP2 had higher body weight (BW) and carcass weight (CW), compared to CC and GT genotypes. However, the differences were not significant (P>0.05). There was significant association (P<0.05) between PRL genotypes and body and carcass weight, where chicken with homozygous DD surpassed individuals with homozygous II genotypes.
The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR...products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.
The aim of the present study was to perform lifetime performance analysis in three pig breeds; Hungarian Large White (n=295), Duroc (n=76) and Pietrain (n=91) on a commercial farm using analysis of survival sows. We took into consideration the age of sows at the time of their inclusion into breeding, their age at the time of culling, time spent... in production, number of mating and parities, parity percentage, intervals between litters, number and mean of piglets born alive and born dead, number of raised piglet litters, number and mean of 21 days old piglets, the weight and mean of raised litter and raise percentage.
We carried out the analysis by SPSS 22.0. Single factor analysis of variants, Kaplan-Meier analysis and Cox PH model were used. The determination of the significance of risk rates differences was done by Wald chi square test.
Our results showed that the average culling age were 1056 (±33.52) days for the Hungarian Large White, 735 (±73.56) days for Duroc and 818 (±71.98) days for the Pietrain.
The log rank test of the survival analysis indicated a significant difference between the three tested genotypes (χ2=16.981, P<0.001), which means that the survival percentage of the individual breeds varied significantly from one another. In comparison with the Hungarian Large White genotype the Duroc genotype has a 1.6 times higher (P<0.001) culling risk while that of the genotype Pietrain was 1.36 times higher (P<0.001).
Our results can be used to compare the breeds kept under the same conditions and to compare the life span of one genotype under different farming conditions. Factors that increase survival and improve the profitability of pig farming can be determined by this method.
One the most effective ways to satisfy the requirements of mankind for food of animal origin is the fattening of chicken. Healthy and cheap meat with low fat and high protein content can be produced rapidly and in large quantities by broilers with low feed consumption. This paper presents the production results and carcass traits of three genot...ypes (Ross 308, Cobb 500, Ross broiler parent) females and males at the age of 42 days.
In both sexes, we found that the average body weight of the Ross parents genotype was higher (females: 2643 g; males: 3089 g) than the
average body weight of the other two hybrids – significant difference was found only between pullets. However, the smallest difference in the
average body weight between the two sexes (446 g) was found in this genotype. In both sexes, the lowest average weight was detectable in
the case of the Cobb 500 hybrids (females: 2443 g; males: 3052 g).The feed consumption ability of the females were worse – in all but one
case (Ross pullet) – than that of the males. For one kg of weight gain - in the order of genotypes – 1.79kg, 2.08kg and 2.08 kg of feed was
used. The same values for the males were as follows: 1.81kg, 1.80kg and 1.89 kg, respectively.
Carcass traits were examined and it was found that the average grillfertig weight, the weight of whole thigh meat and thigh meat of the
Ross parents’ genotype was higher than the average weight of the other two hybrids. Significant difference was found only in grillfertig- and
whole thigh meat weight. Pullets of the Ross parents’ paternal line had worse results in the average weight of breast meat with bone and
breast meat fillet than the other two genotypes. In the case of cocks was detectable the inverse of this: the average weight of breast meat with
bone was the highest in the cocks of the Ross parents’ maternal line.
In order to produce sunflower in Hungary today it is important to develop hybrid-specific cropping technologies. The ever widening number of hybrids makes the constant examination of genotypes necessary from the viewpoint of genotype-environment interactions and critical elements. Plant density as a complex factor puts strain on the pathologica...l features, yield and quality of sunflower. The experiment’s main objective is to find the optimal plant density for both the genotype and external factors.
As a result it can be stated that the optimal crop density is between 45,000-75,000 plant/ha. In 2001 the optimal density was 55,000 plant/ha. The Aréna PR and the Alexandra PR hybrids produced the greatest yields (3511 kgha-1; 3338 kgha-1). In the growing season of 2002, the yields were higher than in the previous year and the optimal crop density was 45,000-65,000 plant/ha. The best yields were produced by the Aréna PR and Alexandra PR hybrids in this year again (4102 kgha-1; 4267 kgha-1) and in 2003, 45,000-65,000 plant/ha proved to be the best crop density. The highest yield was produced by the Alexandra PR.
Analyzing the growing seasons of 2001, 2002 and 2003 it can be declared that as a result of dry climate of the three years yields were higher. It can be stated that the yield is decreased by higher than average of precipitation in the growing season.
The chlorophyll content (SPAD), leaf area index (LAI) and leaf area duration (LAD) of three winter wheat varieties have been investigated on a chernozem soil. Three winter wheat varieties (GK Öthalom, Pannonikus and Genius) in three different nutrient-supply levels have been involved in our experiment in the crop-year of 2010/2011. The investi...gated physiological properties have been set against to the yield results. Upon the results of this comparison it has been stated that there is a close relationship between the investigated physiological properties and yield averages. The SPAD-values showed a growing tendency parallel to the growth of the nutrient-supply levels. The varieties have the highest SPAD-values at the nutri ent-level of N120+PK. Compared to the yield averages we have stated that varieties that have higher SPAD-values have realized higher yields as well. Higher nutrient dosages have increased in the leaf area. In case of all the three varieties the highest LAI-values were measured at the flowering stage (23. 05.) in the treatment whit N120+PK. Varieties with higher leaf area have produced higher yields as well. Parallel to the increasing nutrient-supply levels the value of leaf area duration increased as well. We measured the highest LAD-values in the treatment of N120+PK nutrient-level. By the LAD-values it can be stated that more durable and larger leaf area has been produced in the flowering and ripening phenophases, due to the higher nutrient-dosages. Analysing the relationships between the results it can be stated that there is a strong positive relationship between fertilizer treatments and SPAD-, LAI- and LAD-values. The genotype showed a strong positive correlation to SPAD-values. Yield averages showed strong positive correlation to SPAD-, LAI- and LAD-values as well.
The author investigated 153 lambs of nine genotypes originating from breeding flocks, and 50 lambs originating from production flocks. The investigations were performed between 1995 and 1998. The authors discuss the evaluation of comformation and fat cover according to EUROP standards. The authors also investigate the proportion of valuable mea...t by genotype, and – out of the internal value indicators – the dry-matter, protein, fat, connective-tissue and hemin contents comparing the flavour, aroma, tenderness, and oven loss of the different genotypes.
The following findings should be highlighted:
♦ The Hungarian Merino breed should be improved, as – according to EUROP standards, more than 70% of these animals were rated as quality „R”.
♦ Hungarian fattening technology has to be preserved, as the lambs reach the desired slaughter weight within a short period of time, and without over-fattening.
♦ Readiness for slaughter, typical of each genotype, has to be defined, and slaughter at proper weight be achieved.
♦ It has to be re-evaluated whether the Hungarian Merino is the only breed which can be used in Hungary, as none of the investigations really proved the special characteristics and significance of this breed.
The author summarizes the findings of the investigation in five tables.
Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland... vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
In this study, we selected three hybrids (Armagnac, Loupiac, and Sushi) for evaluation of the effect of traits in genotype in Debrecen. In 2017, the total rainfall from May to October was 314 mm in Debrecen, which was 236 mm in the winter period before sowing. The obtained results showed that there was a positive correlation between the weight...of the cob maize and the rate of seed/cob, number of rows with number of seeds in column and outer diameter ear with weight of cob and number of rows in grain per ear and the rate of seed / cob; Also, there was a negative correlation between grain weight in ear with seed/cob rate, outer diameter ear and the rate of seed/cob and outer diameter ear with the number of leaves. There was a positive correlation between stem diameter, Seed/cob rate and the number of nodes by GGE biplot. In addition, there are traits of weight of all seeds and outer ear diameter that had the highest effect on average yield. Moreover, the number of seeds per row showed the least effect on the average yield of hybrids.
The millet is a very special plant with good adaptation that gives the possibility for the late sowing and secondary production. However the effects of late sowing modifies the efficiency of the agrotechnological elements. The exainations – focused on this aspect – was conducted in the DU RINY and DU RIK in small plots in four replications...in 2014. Among the examined factors (sowing time, nutrient supply, growing area) sowing time had the largest effect. The effectiveness of the agrotechnological elements decreases under unfavourable circumstances caused by the late sowing.
The agrotechnical elements modifies the yields in the examined genotype. Sowing time had the biggest effect on the yields of millet. The genotype „Maxi” had the highest yield in the different treatment variations.
The yield differences were significant between the sowing time and plant density variations, but the nutrient supply had not significant effect (the rate of precipitation was unfavourable in 2014 season).
H-FABP, LEPR and MC5R genes were suggested as candidate genes for fat content in pig meat. The aim of this study was to detect genetic variation in the porcine H-FABP, LEPR and MC5R genes by PCR-RFLP method in a group of pigs. Genotyping of pigs was done by PCRRFLP methods. We identified three genotypes in the set of pigs, HH (0.504), Hh (0.412...) and hh (0.084) for H-FABP (HinfI). Allele H showed higher frequency than allele h (0.710 vs. 0.290). Three genotypes were identified for the H-FABP (HaeIII) gene (DD - 0.194, Dd - 0.494, dd - 0.312). The allele D (0.441) showed slightly lower frequency than allele d (0.559). All three genotypes were identified for LEPR (HpaII) in the group of pigs (AA – 0.137, AB - 0.314, BB – 0.549). Higher frequency of LEPR gene was confirmed for allele B (0.706), as compared with allele A (0.294). We identified two genotypes for MC5R (BsaHI) in the group of pigs (AA - 0.348 and AG - 0.652), genotype GG was not found. As conforms with genotype structure, we recognize a higher frequency of allele A (0.674) as compared with allele G (0.326).
...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">Authors estimated the genetic parameters and genotype effects of average daily gain (ADG), age (AGE) and lean meat percentage (LMP) using the field test data of Pietrain (Pi), Duroc (Du), Hampshire (Ha) pigs and their crosses. Data was collected by the Agricultural Agency of Administration between 1998 and 2010 originating from 68 herds. Datasets of the different crossing combinations (Pi, Du, Pi × Du; Pi, Ha Pi × Ha) were evaluated separately using bivariate animal models. The estimated heritabilities were moderately low: 0.24–0.29, 0.22–0.26 and 0.18–0.19 for average daily gain (ADG), age (AGE) and lean meat percentage (LMP), respectively. The estimated genetic correlation coefficients were negligible: -0.07–0.01 (ADG-LMP), -0.01–0.04 (AGE-LMP). The Pi × Ha and Pi × Du crosses showed 6.76% and 4.96%; 6.74% and 4,17% and 0.08% and 0.44% heterosis for ADG, AGE and LMP, respectively. Among the environmental factors the herd effects were substantial: 41.17%, 53.67% and 14.16% for AGD, AGE and LMP, respectively. The smallest environmental influences were found for LMP.
Today, tomato has the second largest harvesting area globally, and the fourth largest in Hungary. The importance of industrial tomato is growing year by year, and one of the most important tasks is to satisfy the needs of the industry. To meet these needs, the producer has to find the proper genotype, which can achieve up to 100 t ha-1...p> crop yield. This quality has high importance to be researched.
The experiment was carried out in the Botanical and Exhibition Garden of the University of Debrecen, with 3 examined genotypes. The planting date was 3 of May 2018. The harvesting was two-threaded. On 27 of July the physical parameters – fruit shape index (length/diameter), firmness (kg cm-2), water-soluble dry matter content (Brix%) and weight of the berries (g) – were evaluated. On 15 of August the rheological parameters were examined – the force needed to tear the skin – bioyield point (g) and flesh firmness (g).
The fruit shape index was oval (between 1.19 and 1.24) for all the examined genotypes. The firmness was between 0.92 (UG1122713 F1) and 1.05(Prestomech F1) kg cm-2, which compliance the needs of the industry. The water-soluble dry matter content was suitable, with a value between 4.49 (Prestomech F1) and 4.65 (UG1122713 F1) Brix%. The weight of the berries was between 45.94 (UG989 F1) and 49.37 (Prestomech F1) g per piece.
The bioyield point was between 76.00 (UG1122713 F1) and 85.16 (UG989 F1) g. The flesh firmness was between 33.68 (UG1122713 F1) and 42.81 (UG989 F1) g.
Finally, we can conclude based on the obtained data that the UG989 F1 hybrid was the most proper for the applied cultivation conditions. This genotype can be recommended for the farmers even in extreme weather conditions.
Caprine Arthritis Encephalitis (CAE) is a group of diseases in goats caused by a retrovirus, namely the Caprine Arthritis Encephalitis Virus. CAEV belongs to the lentivirus group within the retrovirus family. The CAEV is intimately associated with white blood cells, therfore any body secretions which contain white blood cells are a potential so...urce of virus to other goats in the flock. Once goats are infected with CAEV, it remains infected for life and has to be eliminated.
Since not all goats infected with CAEV progress to disease, very important to test goats for infection using a serology test (ELISA, AGID, IDT). These serological tests demonstrate the presence of antibodies to CAEV in goat serum or detect the virus genome in the white blood cells. However, Swiss results (Dolf and Ruff, 1994) pointed out individual and species differencies in predisposition for this disease. This result was considered as a starting point to our examination. Microsatellite analysis was used in order to find whether there was any association between genotype and serological results, and to look for a marker associated with this disease. To date, altogether 135 goats have been examined. Unfortunately, a significant association between serological results and genotype was not found using the Chi2 test
Maize is the crop that is produced on the second largest area in our country, in Hungary. It is planted on nearly 25% of the country’s growing area and it was produced on 1 090 439 hectares in 2016. Despite the continuous development of the biological basis and production
technology, the growth of the yield results is not constant, its flu
The results of the extensive tests, done between 2009 and 2015, showed that the genotype, the year effect and the plant density are in strong correlation with each other determining the yield results. In the past seven years the examined genotypes reached the highest yield
performance at the highest plant densities. The early hybrids (RM90–95, FAO 200–300) are capable of producing them at higher plant density, while in case of the mid and late maturity varieties the further increasing of the density after reaching the optimum level led to yield depression.
According to our experimental results, the yield is in close positive correlation with the increase of the plant density. The effect of the growing season has great significance in forming the yield results and this determines the applicable plant density too.
The yield of maize is determined by a resultant of components. The main component is the number of ears per plant and the amount of kernels per ear, which is calculated from the number of kernels on an ear and the weight of them. The number of the kernels on an ear is
calculated from the number of rows on the cob multiplied by the number of seeds in one row on the cob. In dry years, at lower yield levels the yield decreases because of the shorter ears, while at the higher levels the number of kernels in a row and the thousand-kernel weight decreases,causing yield depression this way. From our examinations it turned out that the plant density reaction of a genotype is individual, every variety reaches its maximum kernel number per hectare – in other words the maximum yield - in an individual way.
According to our scientific results we can state that we have to use integrated pesticides management in crop protection against the diseases of winter wheat. One of the most important elements of IPM is to select a genotype characterised by good resistance to diseases (and by high yield ability and excellent baking quality). It is especially i...mportant that the wheat variety have tolerance against not only to one or two leaf and spike (grain) diseases, but „complex” tolerance. It is not necessary to give up the growing of a variety which has susceptibility to different diseases because we can protect it using appropriate chemical management. In the intensive growing stage of wheat (BBCH 32-37) we can use a noncompulsary fungicide-treatment (depending on e. g. the infection, ecological conditions) and, at the beginning of the flowering stage
(BBCH 59-65), we have to use a compulsary fungicide-treatment (in spite of e. g. special weather conditions, resistance genotype)to ensure high yield and good quality.
The effect and interaction of crop production factors on maize yield has been examined for nearly 40 years at the Látókép Experiment Site of the University of Debrecen in a long-term field experiment that is unique and acknowledged in Europe. The research aim is to evaluate the effect of fertilisation, tillage, genotype, sowing, plant densit...y, crop protection and irrigation. The analysis of the database of the examined period makes it possible to evaluate maize yield, as well as the effect of crop production factors and crop year, as well as the interaction between these factors.
Based on the different tillage methods, it can be concluded that autumn ploughing provides the highest yield, but its effect significantly differed in irrigated and non-irrigated treatments. The periodical application of strip tillage is justified in areas with favourable soil conditions and free from compated layers (e.g. strip – strip – ploughing – loosening). Under conditions prone to drought, but especially in several consecutive years, a plant density of 70–80 thousand crops per hectare should be used in the case of favourable precipitation supply, but 60 thousand crops per hectare should not be exceeded in dry crop years. The yield increasing effect of fertilisation is significant both under non-irrigated and irrigated conditions, but it is much more moderate in the non-irrigated treatment.
Selecting the optimum sowing date is of key importance from the aspect of maize yield, especially in dry crop years. Irrigation is not enough in itself without intensive nutrient management, since it may lead to yield decrease.
The results of research, development and innovation, which are based on the performed long-term field experiment, contribute to the production technological methods which provide an opportunity to use sowing seeds, fertilisers and pesticides in a regionally tailored and differentiated way, adapted to the specific needs of the given plot, as well as to plan each operation and to implement precision maize production.
The effect of crop production factors on the grain yield was analysed on the basis of three-factorial experiments laid out in a split-split-plot design. In the case of maize the studies were made as part of a long-term experiment set up in 1980 on chernozem soil with forest residues, well supplied with N and very well with PK. The effects of fi...ve N levels in the main plots and four sowing dates in the subplots were compared in terms of the performance of four medium early hybrids (FAO 200). In the technological adaptation experiments carried out with durum wheat, the N supplies were moderate (2010) or good (2011), while the P and K supplies were good or very good in both years. Six N top-dressing treatments were applied in the main plots and five plant protection treatments in the subplots to test the responses of three varieties.
The results were evaluated using analysis of variance, while correlations between the variables were detected using regression analysis.
The effect of the tested factors on the grain yield was significant in the three-factorial maize experiment despite the annual fluctuations, reflected in extremely variable environmental means. During the given period the effect of N fertilisation surpassed that of the sowing date and the genotype. Regression analysis on the N responses for various sowing dates showed that maize sown in the middle 10 days of April gave the highest yield, but the N rates required to achieve maximum values declined as sowing was delayed.
In the very wet year, the yield of durum wheat was influenced to the greatest extent by the plant protection treatments, while N supplies and the choice of variety were of approximately the same importance. In the favourable year the yielding ability was determined by topdressing and the importance of plant protection dropped to half, while no significant difference could be detected between the tested varieties. According to the results of regression analysis, the positive effect of plant protection could not be substituted by an increase in the N rate in either year. The achievement of higher yields was only possible by a joint intensification of plant protection and N fertilisation. Nevertheless, the use of more efficient chemicals led to a slightly, though not significantly, higher yield, with a lower N requirement.
In this study our aim was to find out if there is a difference between the genotypes determined for the previously identified mutations of seven genes of the Hungarian Large White in terms of the time spent in production. We identified the previously determined alleles of the seven genes (BF, EGF, ESR, FSHβ, H2AFZ, LEP, PRLR) related to prolif...eration that were and performed the survival analysis between breeds indicating the risk of culling and the time spent in production on the given farm. Based on the results of survival analysis by Log-rank test, Breslow (Generalized Wilcoxon) and Tarone-Ware test we concluded that they indicated a significant difference in case of the genes BF (Breslow and Tarone-Ware tests) the EGF (Log-rank and Tarone-Ware tests) and ESR (Log rank test) based on which the curves of the survival of the certain genes varied form one another significantly.
...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">The aim of this study was to define the connection between the leptin (LEP) gene T3469C polymorphism and its potential association with production traits in improved hybrid pigs. The study included data from 397 gilts and barrows from 2 different sample. The polymorphism was identified by using the polymerase chain reaction-restriction fragment-length polymorphism (PCR–RFLP) method with HinfI restriction enzyme. Two alleles of LEP gene were identified: T (0.93) an C (0.07). The analysis of values of production traits, depending on LEP genotype did not reveal significant (P≤0.05) differences. In the examination the loin diameter (between the 2nd and 3rd ribs), the live weight at slaughter and the averege daily gain during fattening were higher at pigs with C allele than pigs with TT genotipe. Accordingt to our data the effect of C allele was favourable in this population, because these animals had bigger bodyweight without valuable change of lean meat percent.
The integration of plant breeding and plant transformation is needed because we have to create a homozygous genotype of great agronomic value by conventional breeding before the application of genetic technology with which we modify it by using a gene or genome sequence. The aim of integrated plant breeding is to trigger such advantegous change...s by genetic technology which can not be achieved via conventional breeding or just with considerably weaker efficacy. By transformation, the plant’s agronomic performance, the efficiency and security of its production will improve and it will enable more versatile uses of the plant. Genetic technology is one sequence of a new plant variety’ breeding. To create a transgenic variety, the isolation of a gene or a sequence of a gene from the donor genome for tranformation, a homozygous plant or target genome that is suitable for transformation and is created via conventional breeding methods, an effective transformation technique and the establishment of the new variety from the transformed, fertile plant are needed. The transgenic plant should be made suitable for establishing a variety by conventional breeding so that it could be produced securely, its growing could contribute to the development of modern, sustainable agriculture, its seed could be produced profitably, it could meet the reqiurements of DUS and that the changes indicated by the transgene could provide such economic advantages compared to the original variety, which have real commercial value.
Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolut...e chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
Mineral supplementation is very important in high producing farm animals. The estimation of exact mineral intake is very difficult in forage eating animals, like sheep. Accessing of long term mineral status seems to be possible using wool mineral analysis. However several factors can affect the results. Therefore, the aim of this study was to t...est the effect of breed and sampling location on the mineral content of sheep wool. 20 Dorper and 20 Tsigai sheep were chosen from the same farm. Samples were obtained from 3 locations (withers, side and quarter) and tested for 8 elements: Ca, Mg, Na, Co, Cu, P, S, Se , Zn. The samples were cleaned with ethyl alcohol from organic contamination, then after adding nitric acid were mineral analized using ultrasonic cleaning unit. The samples were analysed with ICP-OES (Perkin-Elmer, Optima 3300 DV). Statistical analyses were carried out by GLM procedure of SAS statistical analyses software. Differences between means were checked with Tukey-test. Significant breed differences were detected in the case of Mg, Na, S, Se in spite of the same feeding regime. The wool mineral content were within the reference range. The sampling location had no effect on the mineral content of wool.
Most domestic maize production products are sold on markets abroad. Among the increasingly restrictive quality requirements, the demand for the measurement of test weight has also appeared. This measurement is not unfamiliar in the case of other cereals, such as wheat and barley, but it has not been applied widely in maize. It is likely for thi...s reason that we have such little information and research available on this topic. In this study, we show the current state of this field with references from domestic and international literature.
The density of maize is the weight of a particular volume and the most frequent unit is the test weight (kg/hl). This physical quality factor plays important roles in the storage, transport and mill industries. The value of test weight is influenced by many factors. The most important ones are the moisture content of grains, drying temperature, drought, precipitation, early frost, and the hybrid characters of a given genotype (grain type, FAO number). In general, the grain with higher moisture content has lower test weight and the higher temperature during (above 82°C) desiccation also leads to unfavourable values. Factors such as a drought interval after flowering, early frost in the case of hybrids with higher FAO numbers, injuries by insects, as well as fungal infections also influence the structure and moisture content of the maize grain.
In the future, broader studies (hybrid testing, application of new agrotechnical elements) will be needed for understanding of the factors effecting test weight.
...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">The H-FABP gene was defined as a potential candidate gene influencing the fat deposition traits, primarily the intramuscular fat content. The aim of this study is to define whether the previously reported gene mutations are connected with the slaughter traits measured in a standard slaughterhouse. The study included data from 405 gilts and barrows from 2 different samples. The two chosen mutation (HFABP1: c. 103 T>C, HFABP2: c. 1970 T>C) were detected in one reaction with PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism) method with HinfI restrictoin enzyme. The allel frequencies are as follows: 103T(H)=0.75; 103C(h)=0.25, 1970T=0.32; 1970C=0.68. A HFABP1 mutation has significant effect on backfat thickness and lean meat % at stable 1 (sample 1), but there were no effect at stable 2 (sample 2). The analysis of values of production traits, depending on HFABP2 genotype did not reveal significant differences. Based on this study we can’t get a clear conclusion on the impact of polymorphisms on production parameters. In the examined flock the allele frequency of mutation in 5 'UTR is identical to the literature data, i. e. the more favorable variant regarding the intramuscular fat content is predominant in the population.