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The importance of predator species in the population dynamics of the Brown hares (Lepus europaeus, Pallas 1778) – Literature review
43-49Views:174One of the conditions for successful small game management is the good management of predator species. The predator species play an important role in the sustainable utilization of the domestic brown hare populations. A portion of these species are under nature protection and with the rest of the species can be utilizing by the wildlife management professionals. Important prey species of brown hares: perspective are red fox, domestic dog and domestic cat. Based on latest date of the National Game Management Database in hunting bags increasing every year the number of the European badger, the stone marten and the golden jackal. In Hungary the brown hare’s most important predator bird species are common buzzard, marsh-harries and goshawk. The human race is not only as a top predator affects the number of the population of brown hares with the wildlife management but indirectly with traffic, (soil cultivation, mowing, and pest control) as well. The control of predators is absolutely necessary for successful small game management, but without sufficient habitat size and habitat development it is hardly sufficient.
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Comparison of Scab Warning Systems in Integrated Apple Production
53-57Views:55In the present study, the suggestions for infection risk and sprays of the most frequently used PC-based scab warning systems (RIMpro and WELTE) and the conventionally used Mills table as a reference were compared in integrated apple production. The efficacy of the spraying programs based on the three different warning systems was evaluated by assessment of apple scab incidences during primary infection periods.
13 and 11 Mills infection periods were detected in 2000 and 2001, respectively, during primary infection periods (from 15 March until 5 June). Taking into consideration the heaviness of Mills infection periods, 11 and 9 sprays were applied in 2000 and 2001, respectively. The two PC-based scab warning systems detected 1-4 less infection periods and suggested 1-3 less sprayings compared to the numbers of infection periods and sprays according to the Mills table. Our results proved that the 9-11 sprays according to the Mills table could be reduced to 7-9 sprays by RIMpro scab warning systems which did not reduce the efficacy and did not increase the symptoms of leaf and fruit scab significantly. The WELTE scab warning systems detected generally one or two more infection periods and suggested one additional spray compared to RIMpro warnings and sprays. -
The effect of the plant density for the yield of the maize hybrids
50-61Views:87In order to enchance the yield stability of maize, the effect of plant density on yields was studied on a typical meadow soil in Hajdúböszörmény between 2002-2004. In the plant density experiment, we used the method of Béla Győrffy. The plant densities applied therefore 20 to 100 thousand plants/ha by ten thousand scale. The application of fertilizer rates for the maize hibrids in every year were N: 110 P: 90 K: 120 kg/ha. We used a manual soiling-gun in the experiment. In every year we used plant protection techniques against monocotyledonous and dicotyledonous weeds. The harvest was done by hand. The facts were read by variancie analysis and linear regression analysis. The moisture and the temperatures were extreme in 2002, 2003, 2004. We have to mention defficiery of moisture in 2003 which is shown that the hot days number increased. After evaluating our findings we can conclude that most hybrids showed a significant correlation between increased plant density and the volume of yields. On the basis of the experiments we divided the hybrids into four groups: the first group included the hybrids suitable for increased plant density with a wide range of optimal density values; the second group included hybrids, which did not require high plant density, were capable of good individual performance and tended to grow several ears; the third group included flexible corn types, which grew longer ears in favourable years, thus yielded more; and the fourth group included the hybrids, which were sensitive to increased plant density and which showed a narrow range of optimal density values. Finally, plant density determines the yield; we have to consider optimal plant density intervals as well as optimal plant density, and we also have to place a high emphasis on the use of hybrid-specific technologies.
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Validation of the competence profile of agri-environmental engineering bachelor course
63-71Views:149As member of the European Union, the development if the Hungarian agriculture is determined by the Common Agricultural Policy. After the reform of the Common Agricultural Policy in 2003, the environmental protection has got a crucial function. The constant changing role of agriculture has reacted students in higher education. For firms connected to agriculture, human resource management based on competency has an important role in custody of competitiveness. One of the most important goals of firms is to supply the demands of their economic partners. To make it successfully, highly qualified human resource is required. The quality of human resource is determined not only by its qualification, language skills, professional experience, and practise, but extant competencies that can also be developed. To choose the expectant employee, it is not enough for them to have intelligence and proficiency; it is also decisive to examine their particular competencies. All of these will define the employee’s
achievement that will definitely contribute to the success of the enterprise. Basically, closer cooperation is needed between higher education institutions and employers to achieve this goal. At the same time, the higher education institutions can revise their training systems considering the actual requirements of labour market, and accordingly, they can form syllabus in a way to train auspicious experts. In this article examination of the requirements of labour market for graduates from agri-environmental engineering bachelor course is presented. -
Slight damage of the great green bush-cricket (Tettigonia viridissima) (Orthoptera: Tettigoniidae) in some Hungarian maize fields
65-70Views:215Characteristic cricket damage was observed in two maize fields in northern Hungary, at Máriabesnyő, a district of Gödöllő. The damage level of the two fields did not differ significantly and continual monitoring of field1 showed also a stable infestation level. T. viridissima nymphs and a female were found and observed as feeding on maize plants. The crickets must have disappeared after 18.07. because no more fresh damage was observed after this date. The chewing’s number about on one and two % of the examined plants amounted one and six a plant and their size was between one and eight cm2. This infestation was quite little and might have caused apparently no yield loss. Compared this damage of T. viridissima with former Hungarian experience, this was the usual negligible damage despite the explicit draught in July and August 2015. As regards the global warming, orthopteran damage may be more obvious in the future.
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Some population genetics parameters of the present Hungarian Hucul Horse population
15-22Views:150We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.
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Loss and Disease Development of Monilinia fructigena (Aderh. & Ruhl.) Honey in an Organic Apple Orchard
6-8Views:145In a two-year-study, yield loss and temporal development of incidence of Monilinia fructigena were quantified in organic apple orchards and the importance of fruit wounding agents was determined. The first infected fruits were observed at the beginning of August in 2001 and 2002. Disease development was continuous until fruit harvest in both years. Pre-harvest yield loss caused by Monilinia fructigena amounted on average 27.2% in 2001 and 41.6% in 2002 by fruit harvest. The growth rate of disease development was almost double in 2002 compared to 2001. All infected fruits were injured by wounding agents such as aboitic and mechanical injury factors, codling moth (Cydia pomonella), common earwig (Forficula auricularia) and birds. In this study, the most important wounding agents were codling moth and mechanical injury factors in organic apple orchards. In both years, our results showed that 70-80% of the infected fruits were damaged by codling moth in organic apple production. Moreover, 10-15% of the infected fruits were mechanically injured in the two years. Our results indicated that most of the damaged fruits fell on the orchard floor before harvest and they became an important secondary inoculum source of M. fructigena. Biological and practical implications of the results are discussed.
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The aggregate stability of the soil in respect to the uniform aggregate stability indicator
83-99Views:86Soil structure and its quality are fundamental properties because they control many processes in soils. Tillage, crop and other factors influence soil structure. Efficient protection of it needs indication of changes in soil structure. A new Normalized Stability Index proposed by Six et al. (2000) tries to evaluate these changes, which was compared with some former used indices. The most common method (wet sieving) was modified to reduce the confounding effects of different particle size distribution of different soil types and method used to the investigation. Changes in soil structure caused by tillage and crop management therefore have been made quantitative and comparable. In this paper, we review the new method and Normalized Stability Index proposed by Six et al. (2000) and present the results of our investigations.
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Physiological examination of some industrial wastes under laboratory conditions
241-246Views:221I would like to draw the attention to the different side-products and wastes that contain lots of organic matter, micro and macro elements, and the fact that they do not have any harmful effect. These materials can be used as micronutrient fertilizers, therefore quantity of the produced CO2 and other greenhouse gases will decrease. Compost, sewage sludge and lime sludge were used in our experiments. The usability of these materials in crop production was examined in crop production within laboratory conditions.
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The noise monitoring system of Debrecen International Airport and requirement of appointing noise protection zones, regarding to the growing air traffic
191-194Views:107The Debrecen International Airport has been undergoing a significant transformation regarding to the growth of traffic and its composition. Considering the dwelling zone location of the airport we cannot avoid the possible noise pollution that it may cause, since its permanency, and growth of intensity, could be the reason of durable health deficiency. The airports’ noise pollution zones are determined, and classified by domestic, and international regulations, the measured data of the noise monitoring system, and the content of the air traffic regarding to the type of planes. Summoning all of these we recommend the D zone qualification of the airport, and the traffic daylight restriction referring to the S5 type of planes. We also advise to build up a more sophisticated and wider extended noise monitoring system.
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Comparison of apricot cultivars for suitability for organic growing
63-65Views:60Nowadays, important task is using more widely the environmentally friendly production technologies. There are considerable differences
among technologies using reduced spray programs (integrated and organic) in plant protection and plant nutrition.
In this case integrated production has a better position as roles for organic production more strict and due to this fruits has an obvious
temporal and permanent reduction in tree conditions. Fitotechnical elements and renewal and regeneration of cultivars are important
factors for offseting or delaying of condition weakening.
In this study, 5 apricot cultivars (not pruned for 8 years) were compared in their characteristics (assessment of inactive, semi-active and
active plant parts).
Our study showed that there were 50-70% differences among observed characteristics. Our study confirmed those cultivars which are
suitable for organic production and which one more resistant to condition weakening and which one able to tolerate negative technological
effects. -
Economic Assessment of Biodiesel Production for Hungarian Farmers
72-76Views:74Utilisation of oil of plant origin as a fuel is gaining acceptance in the European Union and elsewhere. Besides environmental protection, energy saving, and decreasing over-production of food. Additionally, the subsidisation of farmers and the development of rural sub-regions also contribute to its spread. This study specifically focuses on the direct effects biodiesel's raw materials and final products are now having on farmers, while reviewing and quantifying these effects. I have purposely restricted my analysis to these two elements of the biodiesel chain.
The biodiesel chain seems to be a great method for improving the economic and social position of participant farmers in many ways. Presently, the profitability of raw materials’ production looks to be the crucal point in the chain, and could be strengthened best with intensive, habitat-specific agrotechnic. It would only be possible to reach a favourable profit margin for farmers if yields reach unrealistic averages or if there is a significant hike of the 2000 producer’s price in the oil plant branch.
The main attraction of sunflower- and oilseed rape production lies in the stabilization of market conditions, which is not only gong to appear in oil plant branch but – thanks to the reduction of outputs – also in the cereal branches. Better economic safety for farmers may play a role at least on the same level as in plant production, which involves more risks than profit maximalization.
The reduction of the prime cost of biodiesel could be possible through the direct combustion of the whole oilseed plant or its residues or electricity production using them. Whereas energy demand for biodiesel production is low (appr. 5%) but it needs subsidization and the prices of natural gas and electrical energy presently look favourable in Hungary. Additionally harvesting and baling of the residues is technically problematic, which is why their use may seem to be reasonable just over the middle or long term. Another possible factor of cost reduction could be the centralization of some partial operations, which needs serious financial resources to reduce amortization cost per product, provided there be several biodiesel projects near each other during establishment. Creation and operation of a logistical system could also be a good method for improving the viability of the biodiesel chain, in order to optimize transport schedule and distances. However there are also some organizational difficulties in this case. -
Using integrated remote sensing methods in the Nagyerdő Natura 2000 area
19-24Views:145The more widely use of GIS, remote sensing technology provides appropriate data acquisition and data processing tools to build several national and international biodiversity monitoring system of environmental protection and natur conservation. The ChangeHabitats 2 is a similar international project, which uses airborne hyperspectral and airborne laser scanning (airborne LiDAR) sources beyond traditional data collection methods to build a monitoring system of Natura 2000 habitats. The goal of our research, on one hand, was to separate the most typical species of trees which can be found in the largest coverage in the research plots of Debreceni Nagyerdő Nature Reserve from field and airborne remote sensing data, use image classification that based on spectral and geometry (height) characteristics of the trees. On the other hand our goal was to evaluate the efficient use of the integration of mobilGIS, airborne hyperspectral and airborne LiDAR data collecting methods to complement or substitut of the traditional, field data collecting methods. We used ArcGIS 10.2 and Exelis 5.0 GIS software for data evaluation, in which the mosaicing, the selection of plots and the spectral image processing were carried out.
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Sensitivity of maize hybrids to common smut under field artificial inoculation conditions
25-28Views:189Common smut disease of maize is one of the most frequent diseases of crop. In the last decades the importance of disease has decreased in feeding maize production, however its importance increasing again nowadays, especially at sweet maize hybrids. The aims of this work was to find hybrids possess of resistance, and to evaluate which ones are more or less susceptible under field artificial inoculation circumstances. Among feeding maizes the less susceptible hybrid was ‘P9578’, and the most susceptible ’NK Columbia’ hybrid, and differences in cob infection between them was significant (8.8%). At sweet corn hybrids the less susceptible was ’Prelude’, while the most susceptible was ’Jumbo’ with very high significant 74.6% differences.
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Effect of hail net on the water potential of an apple orchard
109-113Views:129Apple production has seen a decline in yields in recent years, primarily due to ecological factors such as drought, water stress, water scarcity, uneven rainfall distribution, frost damage and hail damage. Ecological factors that are harmful to the plant can be eliminated by human intervention, irrigation or the use of hail net. In our study, we investigated the positive effects of hail net on the development of plant water potential as the vegetation progressed, in non-irrigation area with temperature and humidity. Water potential values were determined using a field osmometer. Confirmed the positive effect of the ice protection net in the apple orchard of Early Gold and Golden Reinders. Our results were supported by statistical analyzes our results.
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Development of a Decision Assisting Soil Information System in Agriculture
130-133Views:69Hungarian agriculture may be characterized by the industry-like, conventional farming of the past decades. This form of management concentrated exclusively on functions aimed at production. We have to decrease this disproportion, especially when joining to the EU, by reducing the intensive agricultural regions and adjusting to the goals of the 2000-2006 NAEP programmes, forming such an agricultural system which integrates the advantages of both environmental protection and complex agricultural systems based on rational foundations. Nevertheless it’s a rational decision to make the soil information system with an exact spatial informatics background as a foundation of agricultural system, because this promotes easy handling as well the connection to possible subsequent modules.
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Agri-environmental subsidies and the National Rural Development Plan
52-59Views:58The financing of agri-environmental target programs which is a prominent area in the EU became possible during the implementation and successful operation of the National Agricultural Environment Protection Program (NAPP) launched in 2002. Through this program we gained experience in the field agri-environmental measures which are financed from the Guidance Section of European Agricultural and Guarantee Fund in the European Union. The agri-environmental measures which are included in the National Rural Development Plan (NRDP) were implemented in Hungary in the fall of 2004 when the farmers handed in their application after the publishing of the related law. The NAPP financing is still active, but not significant since most farmers have chosen NRDP measures.
We are examining the experience of the above programs after studying some theoretical aspects of the agricultural economics and the EU laws. We try to analyse the most important experiences of NAPP including the legal background, news opportunities yielded by target programs, the financing, organisation, and institutional background. We will present the results taking into consideration the data of the winning applicants. -
Q-PCR analysis of the resistance of Hungarian Botrytis cinerea isolates toward azoxystrobin
41-44Views:76The genes being in the mitochondrial DNA primarily encode the enzymes of cellular respiration. Fungicides belonging to the family of quinol oxidase inhibitors (QoIs) play on important role in the protection against several plant diseases caused by fungi. These fungicides bind to the cytochrome bc1 complex so they block electron transport between cytochrome b and cytochrome c1. This way these fungicides inhibit the ATP synthesis consequently they inhibit the mitochondrial respiration. The QoI resistance has two mechanisms. One of them is the point mutation of the cytochrome b gene (CYTB), e.g. the substitution of a single glycine by alanine at position 143 results in high-resistance. The other is the cyanide-resistant alternative respiration sustained by the alternative oxidase.
In a cell there are several mitochondria. The phenomenon when the genomes of all mitochondria in the cell are identical is called homoplazmy. If in the cell there is wild and mutant mitochondrial DNA this is called heteroplasmy. Whether the mutation in the mitochondria causes fenotypical diversity or does not depend on the dose, i.e. it depends on the percentage of the changed mitochondrials. During our work we investigated Botrytis cinerea single spore isolates which have been collected in 2008-2009 on different host plants. Our goal was to decide whether heteroplasmy influences the level of resistance. We managed to detect the change of the level of heteroplasmy, so the change the level of the resistance due to the treatment with fungicide. -
Testing disease resistance in autumn wheat genotypes by means of field experiments
30-40Views:80According to our scientific results we can state that we have to use integrated pesticides management in crop protection against the diseases of winter wheat. One of the most important elements of IPM is to select a genotype characterised by good resistance to diseases (and by high yield ability and excellent baking quality). It is especially important that the wheat variety have tolerance against not only to one or two leaf and spike (grain) diseases, but „complex” tolerance. It is not necessary to give up the growing of a variety which has susceptibility to different diseases because we can protect it using appropriate chemical management. In the intensive growing stage of wheat (BBCH 32-37) we can use a noncompulsary fungicide-treatment (depending on e. g. the infection, ecological conditions) and, at the beginning of the flowering stage
(BBCH 59-65), we have to use a compulsary fungicide-treatment (in spite of e. g. special weather conditions, resistance genotype)to ensure high yield and good quality. -
Energy Use of Biomass
264-272Views:80In this study, energy utilization of biomass is introduced with a short description of renewable energy sources and utilization possibilities of biomass.
Presently, the necessity of renewable energy sources is increasingly obvious. Among renewables, energy from biomass is to be highlighted, since this allows versatile, cheap utilization of the sun’s energy. In this respect, Hungary has advantages. Direct heat utilization and biogas production are available procedures today, whereas biodiesel and bioethanol are expected to spread in the near future. Biogas production is possibly the most versatile method for biomass conversion: it can produce energy from materials inapplicable for other utilization; at the same time, it is capable of neutralizing harmful wastes; in the end, it produces also valuable fermentative products, from bio-manure useful in agriculture, to pharmaceutical raw materials. -
Economic policy strategy of UNITAB
83-90Views:71Under the leadership of the International Association of Tobacco Producers – UNITAB – there is a struggle going on for stabilizing the situation of European tobacco growers. UNITAB unites the tobacco associations and tobacco growers of all EU countries.
Tobacco production ensures economic stability for more than 100 thousand small- and medium-sized farms in the rural areas of 10 EU countries. In addition, its social importance in the survival of rural communities is emphasized by the 400 thousand direct or related seasonal jobs.
Within the framework of the CAP reform, the European Union is gradually changing the regulations of production. This is based on the decoupling of subsidies, which will be implemented in two phases. Between 2006 and 2009. the decoupling will be 40% and then after 2010, 50% (the other 50% will be used for restructuring within the second pillar). The question is raised, how will the second 50% be restructured to the second pillar. Between regions and how it can be ensured that this support would go to tobacco production.
With its participation in the principles of the European Tobacco Charta, UNITAB targets for production under economically-balanced conditions, the production of products meeting the customer requirements, consideration of the working conditions (especially for seasonal workers) and adherence to the health regulations related to the consumption of the end product already in the production and environmental protection. They aim to ensure the survival of the sector via tobacco growing with sustainable development and high quality. To maintain the continuity, there is a need for the support of regional, national and EU authorities. Future of the sector is dependent upon adaptation of tobacco growers and upon meeting the social expectations.
According to UNITAB, efforts should be made to modify the decisions so that the majority of the funding could be used by the grower. The tobacco industry has been able to effectively fight for its survival. -
Topology in the fruit plantation
253-257Views:167The localization of fruit trees, the topology of the branch structure and the spatial structure of the canopy are important to plan sitespecific agro-ecological and production technology projects in an orchard. The currently used instruments and technologies – in the precision agriculture – give opportunities to obtain these informations. The examinations were carried out in the Study and Regional Research Farm of the University of Debrecen near Pallag with the use of a GreenSeeker 505 Hand Held™ Optical Sensor Unit, and its interface the Trimble AgGPS FmX Integrated Display board computer. The collected spectral data were completed with the 3D point cloud by Leica ScanStation C10 laser scanner. The laser impulse data and the vegetation index values were integrated in a unified 3D system. The integration of the two special data collection system provides new opportunities in the development of precision production technology system. The results could be directly used in phytotechnology, water management, plant protection and harvesting in orchards. Our elaborated method can supply digital high spatial accuracy guidance data for development of the automated machines, which could provide some new developmental way in the immediate future.
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Phytocoenological researches on the grasslands of the middle and inferior basin of Crişul Negru river
41-45Views:189The meadowlands from the middle and inferior basin of Crisul Negru river form an area with rich flora and vegetation suitable for a complex phytocoenological research and an ecologic and bio-economical study of the floor vegetation.
After conducting a number of 80 phytocoenological surveys on the meadowlands near Apateu and Satu Nou villages (Arad county, Romania), respectively near Cociuba Mare and Mărțihaz villages (Bihor county, Romania) there have been identified a number of fifteen vegetal associations from which one is examined in this work.
The paper presents the hygrophilic association Typhetum latifoliae Lang 1973 on the grasslands of the middle and inferior basin of Crişul Negru river, association classified from the coenotaxonomic point of view in the alliance Phragmition communis Koch 1926, order Phragmitetalia Koch 1926, class Phragmitetea australis R. Tüxen et Preising 1942.
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Yield and sward composition responses of a native grassland to compost application
35-38Views:170A major part of the animal products are based on the grasslands, due to the fact that the grassland ecosystems can be found all over the globe. In places where economical and successful crop production cannot be realized, the grassland based animal husbandry can be an efficient way of food production. In addition these ecosystems have an important role in carbon sequestration, and with their rich flora – and the fauna connected to it – in conservation of biodiversity. The protection of nature, and the sustainable agriculture is getting more and more attention in the European Union, but looking at the consumers’ needs, the production of healthy food cannot be neglected either. Because of these facts, the effects of two specific composts - which are officially authorized in organic farming, in Agri-environment Schemes and Natura 2000 programs – on grass yields and sward compositions were investigated in a field trial. The investigation took place in Hungary, on a natural grassland based on solonetz soil. The first type of compost was a natural one (N) without any additional material and the other one was enriched in phosphorus (E). Both was produced by the research institute, made of sheep manure. Three rates of compost (10 t ha-1, 20 t ha-1,30 t ha-1) were tested on 3 m×10 m experimental plots. Every treatments had four replications and both type of compost had four-four control plots too, this way 32 experimental plots were included in the investigations. The yield of the pasture was harvested two-times (in May and in September) and before cutting the plots measurements on botanical compositions were made. Samples for laboratory analysis were also taken. Dry matter yield and crude protein content was measured in laboratory and with the received data the yield per unit area was calculated. Based on the research results we can say that the application of compost in any dose inflicts higher dry material and crude protein yield. The changes were partly due to some positive changes in sward composition, because of the better nutrient conditions. The research results indicate, that use of organic compost can be an efficient way to increase grass yields in a sustainable way.
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Comparative Vertical Migration of Three Different Strains of Heterorhabditis bacteriophora and a Single Strain of Heterorhabditis megidis in Sand at 25°C
47-52Views:79Heterorhabditis bacteriophora and Heterorhabditis megidis) were tested for downward migration in 9.5 cm vertical sand columns at 25°C with and without a larva of the wax moth, Galleria mellonella, at the bottom. The number of infective juveniles (IJs) in the upper section decreased since the IJs gradually migrated down toward the Galleria larvae into the lower section of the shell vials. Only the isolate OH 25 behaved differently, because the number of IJs decreased this isolate in the lower section. This isolate was the quickest, since after 12 hrs, most IJs could be found in the lower section of the vials from this isolate. The number of IJs was so small in the lower section that 12 hrs after injection, only the isolates MHG 3 and OH 25 could kill the Galleria larvae.
Besides the fact that mortality occurred among the Galleria larvae with the above isolates, neither IJs nor adults could be detected in either living or dead Galleria larvae 12 hrs after IJ injection. It is noteworthy that the behavior of the isolate Megidis was different from the other isolates: the number of IJs was so negligible in the lower section of the vials that even after 36 hrs none of the Galleria died and, consequently, neither adults nor IJs could be detected after dissecting the Galleria larvae. Each isolate could reach the lower section of the vials, yet only the isolate Megidis could not infect and kill the host. After dissecting the larvae, most IJs and adults could be found in the isolate MHG 3 (95 IJs and adults altogether) 36 hrs after injection.