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Impact of nitrogen and sulphur fertilization on the growth and micronutrient content of spring wheat (Triticum aestivum L.)
211-219Views:197Micronutrients are as important as macronutrients for crops. Each micronutrient has its own function in plant growth. Zinc is important for membrane integrity and phytochrome activities. Copper is an essential micronutrient required for the growth of wheat. Manganese is required for enzyme activation, in electron transport, and in disease resistance. The pot experiment was set up in greenhouse on calcareous chernozem soil Debrecen-Látókép with a spring wheat. In certain development stages (according to BBCH growth scale of wheat), at the beginning of stem elongation (29–30), at the heading (51–59), at the flowering (61–69) stage three average plants were removed from all pots for analysis. Fresh and dry weight of the plant samples were measured. Plant leaves after drying were digested by HNO3-H2O2 methods and manganese, zinc and copper contents of plant were quantified by atomic absorption spectrophotometry. At the flowering stage, when the nutrient uptake of plants is the most intensive, the weight of wheat ranged between 0.94–1.57 g plant-1. In this development stage, the NS2 treatment produced the highest weight of wheat, and compared to this the NS3 treatment decreased that value already. The results show unfavourable effect of NS3 treatment. On the basis of microelement content of wheat and the weight of a plant, nutrient uptake by plant were calculated. At the beginning of growth the starter treatment had positive effect on Cu-uptake compared to the NS1 treatment, where the same dose of fertilizer was stirred into the soil. Wheat is very sensitive to copper deficiency, so copper dissolved by starter treatment could be favourable to the early development of wheat. At flowering stage the Zn-uptake of wheat became the highest and it was between 133.7–234.6 mg plant-1. The Mn-uptake of wheat plant was higher than the Cu- and Zn-uptake of wheat.
This phenomenon can be explained by the fact that the untreated soil had higher Mn-content, than Cu- and Zn-content. To summarize the results, it can be stated, that the copper uptake of wheat was more affected by the different treatments in the stage of stem elongation, while Mn- and Zn-uptake of wheat were influenced primarily in the stage of heading and flowering.
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The effect of apoplastic pH on the nutrient uptake
65-71Views:107The pH of soil and rhizosphare –around the roots- determine the mobility and solubility of nutrients. The exudates organic acids of plant able to modify the pH, as well as the microorganisms also take part in mobilization of nutrients. The nutrient solve mostly in mildly acidic and neutral pH. The either assumption of utilization of nutrients is the uptake by roots and of course uptake to the cells to take part in metabolism. The pH of apoplast fluid determines the solubility and uptake of nutrients to the cells.
The aim of this study was to examine the effect of nutrient solution and apoplastic pH together with a bacteria based biofertiliser (Phylazonit MC®) on nutrient uptake and pH of apoplast fluid in case of nutrient solution grown plants in laboratory experiment. According to my results, the bicarbonate increased the pH of nutrient solution in due to influence the solubility and uptake of nutrients. The given bicarbonate to the nutrient solution and infiltrated into the apoplazma also modified the pH of the apoplast fluid of the test plants. The effect of bicarbonate and biofertilizer were different on the pH of the apoplast fluid and nutrient solution in nutrient solution experiment. -
Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
109-115Views:204The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms were compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant. The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.
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Effect of molybdenum treatment on uptake of plant and soil molybdenum content in a field experiment
117-122Views:181Molybdenum is not a well-known microelement, but being a constituent of several important cellular enzymes it is an essential microelement. Molybdenum occurs in all foods, but at very low levels. There does not appear to be any particular foods or types of foods, which in the absence of extrinsic factors, naturally have high levels of molybdenum. However, environmental pollution, from natural or anthropogenic sources, can lead to high level of the metal in plants.
Our study is based on the long-term field experiments of Nagyhörcsök, where different levels of soil contamination conditions are simulated. Soil and plant samples were collected from the experiment station to study the behaviour of molybdenum: total concentration, available concentration, leaching, transformation, uptake by and transport within the plants, accumulation in different organs, phytotoxicity and effects on the quantity and quality of the crop. In this work we present the results of maize and peas and the soil samples related to them.
According to our data molybdenum is leaching from the topsoil at a medium rate and it appears in the deeper layers. In the case of plant samples we found that molybdenum level in the straw is many times higher than that is in the grain, so molybdenum accumulates in the vegetative organs of the plant. The data also show differences in the molybdenum-uptake of cereals and Fabaceae (or Leguminosae). -
Biostimulant induce growth, chlorophyll content and fresh herbage yield of alfalfa (Medicago sativa L.) and variegated alfalfa (Medicago × varia Martyn) plant
19-25Views:109The use of biostimulants is associated with promoting plant growth by stimulating cell division and improving nutrient availability and uptake. A study was conducted at the University of Debrecen, Hungary, to examine the effect of biostimulants on alfalfa growth, chlorophyll content and fresh herbage yield. The experiment was arranged in a randomised complete block design with three biostimulant treatments plus control replicated three times. Data collected were subjected to analyses of variance using Genstat, where significantly different means were separated at a probability of 5% using the least significant difference. The findings show no different variation in plant height or chlorophyll content (SPAD) throughout the early stages of growth. Nonetheless, a notable impact was noted in the latter stages (28 days after biostimulant treatment application) on the growth of the alfalfa plant. Biostimulant treatments did not had effect on fresh yield for second through fourth cuts, but the fifth cut showed a significant effect, with T1 treatment recording the highest herbage yield of 19745 kg ha-1 followed by T2 (Tricho Immun plus Ino Green) and T3 (Tricho Immun), with yields of 19528 kg ha-1 and 17273 kg ha-1, respectively, while the T0 (control) recorded the lowest herbage yield of 12060 kg ha-1. However, the average mean yield indicated the application of biostimulants significantly increased fresh yield herbage by 20.5%. Correlation coefficient values suggested plant height at both 14 and 28 DAH (days after harvest) strongly correlated with fresh herbage yield (r = 0.7756 and 0.7455) which reflected in the increase in fresh herbage yield. Therefore, our results suggest that the use of biostimulants in alfalfa cultivation holds promise for improving growth and yield potential through their positive effects on chlorophyll content and the growth of alfalfa plant.
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Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment
77-85Views:152The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the changes of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.
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Effect of molybdenum treatment on the element uptake of food crops in a long-term field experiment
75-79Views:167Molybdenum, as a constituent of several important enzymes, is an essential microelement. It can be found in all kind of food naturally at low
levels. However, environmental pollution, from natural or anthropogenic sources, can lead to high levels of the metal in plants. Our study is based on long-term field experiments at Nagyhörcsök, where different levels of soil contamination conditions are simulated. Plant samples were collected from the experiment station to study the behavior of elements: uptake by and transport within the plants, accumulation in different organs, phytotoxicity and effects on the quantity and quality of the crop. In this study, we present the effect of molybdenum treatment on the uptake of other elements. Molybdenum is proved to be in an antagonist relationship with copper and sulphur, while molybdenum-phosphorus is a synergist interaction. However, in most of the plants we studied, increasing molybdenum-treatment enhanced cadmium uptake. We found the most significant cadmium accumulation in the case of pea, spinach and red beet. -
The impact of applying foliar fertilizers on the health condition of maize
105-109Views:194The nutrient supply of plants is becoming more important in plant protection since the appropriate condition of plants can be ensured by optimal and satisfactory nutrient supply to avoid nutritional deficiency diseases. Due to the extreme weather conditions, plants are forced to face several stress factors, which leads to deterioration of the plant health. The increasing occurrence of droughts poses threat to nutrient uptake through the roots since all the nutrients can be accessible to the plants only if they are in dissolved form in the soil – which is not possible in the absence of water. Therefore, the importance of foliar fertilizer is becoming a more and more significant part of the nutrient supply, because with the help of this technology the development of any nutrient deficiency can be prevented.
In this experiment, we focused on the efficiency of two different foliar fertilizers on maize. Foliar fertilizers were applied two times, once in the stage with 8 leaves and tasseling phenophase of the maize. To verify the efficiency of the foliar fertilizers, the chlorophyll content of untreated and treated plant’s leaves was measured after each application. Moreover, the length and diameter of maize cobs, thousand kernel weight, protein, oil and starch content were also measured, and the results were compared to the untreated (control) ones. According to the results, in all aspects significant differences were observed and due to the laboratory analysis of leaves, in the case of magnesium and zinc supply the foliar fertilizers were able to prevent the development of nutrient deficiency.
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Effect of molybdenum treatment on the element uptake of plants in a long-term experiment
121-125Views:149Molybdenum as a constituent of several inmportant enzymes is an essential micorelement. It can be found in all kind of food naturally at low level, however, environmental pollution, from natural or anthropogenic sources, can lead to high level of the metal in plants. Our study is based on the long-term field experiments of Nagyhörcsök, where different levels of soil contamination conditions are simulated. Plant samples were collected from the experiment station to study the behaviour of elements: uptake by and transport within the plants, accumulation in different organs, phytotoxicity and effects on the quantity and quality of the crop.
In this work we present the effect of molybdenum treatment on the uptake of other elements. Molybdenum is proved to be in an antagonist relationship with copper and sulphur, while molybdenum-phosphorus is a synergist interaction.
However, in most of the plants we studied increasing molybdenum-treatment enhanced cadmium-uptake. We have found the most significant cadmium-accumulation in the case of pea, spinach and red beet. -
Practical experiences of a designing and operating a pilot aquaponic system
27-32Views:335Aquaponics is the combination of fish farming (aquaculture) and the soilless cultivation of plants (hydroponics). The aquaponics system is an artificial, recirculating ecosystem, in which bacterial processes convert the waste materials in the water used for fish rearing into plant nutrients, and therefore with the generated heat it is suitable for culturing economically valuable plants, and thus it mitigates the nutrient laden and quantity of the intensive fish producing systems’ effluent water.
The primary goal of our 12 separate unit’s aquaponics system was to gain experience. We would like to find the right plant species, which are fit for that medium, and their crop can be sold. Besides the plants, our attention focused on the fish. Two fish species were included in the experiments, the common carp and barramundi. It was difficult to create them a perfect living space, besides a constantly changing conditions temperature. Apart the above mentioned we had a problem with the number of individuals per tank, the deformity of the fish body and the too high volume of pH (we registered continuous values above 8.4). We get by carps 4.7 grams of weight gain during 15 weeks, because of the bad conditions.
The main problems at the plants are caused by aphesis and protection against sunburn. Even so we have got the multiples of field yields for each plant species. At salad has grown twice of field yields, tomatoes one and half, kohlrabi than 3.5 times more. The causes of multiple yields are the continuous balanced water and the nutrient uptake of plants. Each plant species fit for cultivating in aquaponics and their crops are delicious, chemical -free, safe and marketable. The plants should be more concentrated. After the experiment, it has been determinated that the carp is suitable for aquaponics, but greater weight gain could be achieved with optimal selection of size of rearing units.
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The Effect of Smut Gall Tumour Infection on Iron and Zinc Uptake and Distribution in Maize Seedlings
27-32Views:99The amounts of Fe, and Zn were measured in maize seedlings infected by smut gall tumour (Ustilago maydis Dc. Cda.) and in healthy seedlings five days after infection. The amount of elements was also measured under different stress intensities. Due to the infection, as a biotic stress, the amount and distribution of examined elements have been changed. On the bases of the differences in the Fe distribution between the symptoms less and tumorial leaf parts, we have come to the conclusion that the infection also effects the mobilisation of Fe and Zn inside the plants. The Fe uptake was much higher in the infected plants and the tumour development also had an effect on the uptake and distribution of the examined elements. The experiments of infecting maize seedlings by monosporidial strain of crown gall tumour showed no tumour development. We found that the monosporidial strain also acts as a biotic stress and has an effect of iron and zinc distribution. We observed a slight difference in the iron and zinc contents in the roots of corn seedlings infected by different monosporidial sporidium concentrations, while the iron and zinc contents in the shoots were increased by the intensity of the infection. The roots do not form tumours. There is no difference between the roots of the infected and healthy corn seedlings. Since the Fe and Zn contents of the shoots of infected plants depend on the intensity of the infection, we have come to the conclusion that there must exist a „special” communication system regulating the transportation of the examined elements.
In the experiments with infected maize seedlings, it became necessary to get the iron chlorosis before the disease reaches the lethal phase. Although most of the iron reserves are located in the embryo, to accelerate the chlorosis, the endosperm was removed, and it was observed, that the iron chlorosis appears later in maize seedlings when the endosperm is removed. The relative chlorophyll content of the first and second leaves was measured in iron efficient and iron deficient maize seedlings at different times.
The higher IAA content of tumorial plant tissues is already known. The treatment with IAA decreases the iron concentration in the shoots and in the roots of +Fe precultured plants and increases at -Fe precultured ones. The TIBA retards the shoot-to-root transport of IAA. When the seedlings were treated simultaneously with IAA and TIBA, higher iron concentrations were observed in the shoots and in the roots of corn seedlings.
We found extremely high iron concentrations in the roots of infected seedlings and, in line with this, serious damage to the roots was observed that this can be caused by the high iron content generated free radicals. The results demonstrate that IAA has a role in the shoot to root communication. -
Az NPK-trágyázás hatása a kukorica tápelemfelvételének dinamikájára, öntözött és nem öntözött viszonyok között
23-27Views:183The effect of NPK-fertilization on the dynamics of nutrient uptake of maize (Zea mays L., cv. Clarica) was examined on chernozem soil under irrigated and non-irrigated conditions in a field experiment.
The following results were made:
• the element concentrations in the plant decreased over time,
• there is no difference between the dynamics of nutrients on irrigated and non-irrigated sites because rainfall was satisfactory for plants in vegetation period,
• the N doses not only significantly increase the nitrogen content in maize, but also have a noticable effect on Ca and Mg concentrations,
• because of the acidifying effect of N-fertilizers, increasing the amount of N-fertilizer increased the Mn, Zn, Cu content of the plants,
• the P doses have a significant effect on the maize P and N content and the Zn concentration of the plant via P-Zn antagonism in the soil,
• as the high K doses treatments alter the ion ratios in the soil, the Ca, Mg content of the plant decreased. -
Magnesium uptake dynamism of maize (Zea mays L.) on prairie soil
83-89Views:86Different influence factors on the magnesium (Mg) uptake in case of three maize hybrids with different long vegetation period have been investigated at the Experimental Station of the University of Debrecen, Centre of Agricultural Sciences and Engineering, at Debrecen-Látókép. The soil of the experiment is a calcareous chernozem, based on loess, with high fertility, that is characteristic for soils of the region Hajdúság.
Upon irrigation is the experiment divided to main plots, by different hybrids into sub-plots, while treatments of five nutrientsupply levels with fixed N:P2O5:K2O rate (beside control)mean sub-subplots. Soil samples were taken from the upper, cultivated soil layer 3 times during the year 2008. Their pH has been measures in a 0.01 M CaCl2-solution and their Mg-content from the same solution and from ammonium-lactate acetic acid (AL) extract. Plant samples were taken seven times in the vegetation period, of which we measured the Mg-content. Beside this, the during the
vegetation period by maize biomass extracted Mg-amount has been calculated using fresh and dry matter weights. The effects of irrigation, hybrids and nutrient-supply levels on the soil pH and on the AL- and CaCl2-extractable Mg-amount have been studied, as well. After that I tried to find a correlation between soil pH and the Mg-content of soil determined in different extractants, beside this between the by the two solutions extracted Mg-amount. -
Effects of different groundcover matters on nutrient availability in an integrated apple orchard in Eastern-Hungary
21-25Views:116The aim of our study is to examine the effects of different groundcover methods on nutrient availability and uptake of apple orchard. The
experiment was carried out at the orchard of TEDEJ Rt. at Hajdúnánás-Tedej, in Eastern Hungary. The orchard was set up on lowland chernozem soil in the Nyírség region. It was established in the autumn of 1999, using Idared cultivar grafted on MM106 rootstocks at a spacing of 3.8 x 1.1 m.
The applied treatments were divided into two groups according to origins and effects. On the one hand, different livestock manures (cow,
horse and pig), on the other hand different mulch-matters (straw, pine bark mulch, black foil) were used. The different manures and mulches
were applied on the surface to test the effectiveness of these materials.
The effectiveness of manure treatments was higher than the other treatments on AL soluble P content of soil. Mostly the manure treatments
increased the AL soluble K of soil. Our all treatments increased 0.01 M CaCl2 soluble NO3 - -N content of the examined soil layers. The effect
of manure treatments was the highest. From the results it was evident that the amount of easily soluble organic nitrogen fraction distributed
more homogeneously than the other mineral N fractions examined.
Our results can be summarized as follows:
1. Our results pointed out that the used ground covering matters divided into several categories regarding its effect.
2. The available N, P and K contents of soil were mostly increased by applying manures.
3. The effectiveness of straw, mulch and mostly black foil was lower.
4. Differences were found between nutrient supplying treatments and the treatments which did not supply nutrients. -
Potential use of bamboo in the phytoremediation in of heavy metals: A review
91-97Views:357There are many literature sources focusing on the phytoremediation of woody plants, but there are only few dealing with the phytoremediation of bamboo plants. Phytoremediation technology has the advantages of little disturbance to the environment and low remediation cost. Bamboo mainly exists in tropical and subtropical regions. As an energy plant, bamboo has a fast growth cycle, large biomass, simple cultivation, high economic efficiency, and convenient harvesting, which highlights the advantages of bamboo in phytoremediation. In addition, bamboo plants have good tolerance and uptake ability to heavy metals and have high application potential and development value in uptaking heavy metal contaminated soil. However, due to climate, temperature and other reasons, bamboo cannot be widely planted in most countries. Research status of remediation of heavy metal contaminated soil by bamboo plants is summarized. The feasibility of its application in heavy metal contaminated soil is discussed in this paper. Aiming at the shortcomings of existing research, bamboo plants have a prospect in the field of plant phytoremediation for the future.
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Nitrogen Supplying Capacity of Brown Forest Soil under Different Cropping Practices and 0.01 M CaCl2 Soluble Organic Nitrogen
17-23Views:111The best known and most remarkable example of continuous production in Hungary is the Westsik’s crop rotation experiment, which was established in 1929, and is still in use to study the effects of organic manure treatment, to develop models, and predict the likely effects of different cropping systems on soil properties and crop yields. In this respect, Westsik’s crop rotation experiment provides data of immediate value to farmers concerning the applications of green, straw and farmyard manure, as well as data sets for scientific research.
Although commonly ignored, the release of nitrogen by root and green manure crops has a significant impact on soil organic matter turnover. The design of sustainable nitrogen management strategies requires a better understanding of the processes influencing nitrogen supplying capacity, as the effects of soil organic matter on soil productivity and crop yield are still very uncertain and require further research. In the treatments of Westsik’s crop rotation experiment, nutrients removed from soil through plant growth and harvesting are replaced either by fertilisers and/or organic manure. Data can be used to study the nitrogen supplying capacity of soil under different cropping systems and its effect on the 0.01 M CaCl2 soluble organic nitrogen content of soil.
The aim of this paper is to present data on the nitrogen supplying capacity of brown forest soil from Westsik’s crop rotation experiment and to study its correlation with hundredth molar calcium-chloride soluble organic nitrogen. The main objective is to determine the effects of root and green manure crops on the nitrogen supplying capacity of soil under different cropping systems. The nitrogen supplying capacity was calculated as a difference of plant uptake, organic manure and fertiliser supply.
The 0.01 M CaCl2 soluble organic nitrogen test has proved reliable for determining the nitrogen supplying capacity of soils. Brown forest soils are low in organic matter and in the F-1 fallow-rye-potato rotation, the nitrogen supplying capacity was 15.6 kg/ha/year. 0.01 M CaCl2 soluble organic nitrogen content was as low as 1.73 mg/kg soil. Roots and green manure increased the nitrogen supplying capacity of soil by more than 100%. This increase is caused by lupine, a legumes crop, which is very well adapted to the acidic soil conditions of the Nyírség region, and cultivated as a green or root manure crop to increase soil fertility. -
Investigation of the production parameters, nutrient and mineral composition of mealworm (Tenebrio molitor) larvae grown on different substrates
129-133Views:277During the rearing of mealworm (Tenebrio molitor) larvae, the optimisation of the growing substrate has a particular importance. The application of the appropriate substrate is a fundamental pillar for intensive and safe production. The requirements for substrate include the lack of toxins, high nutrient and micro-macro element content. The aim of this study was the evaluation of the effects of different substrates on the production parameters, nutrient and mineral composition of the mealworm larvae. The experiment lasted through 14 days. 5 treatments were set up at, where the variable was the substrate. The test system consisted of 25 units (5 treatments and 5 replicates). 10 mealworm larvae per unit, (total of 250 larvae) were used at the beginning of the experiment. Regarding the substrates, our study included sweet potato (SP), may turnip tuber (MT) and may turnip leaf (ML). In addition to the plant by-products, wheat flour (WF) and wheat semolina (WS) were used as control substrates. Trace element uptake and production parameters of the larvae were determined at the end of the experiment. Regarding the production parameters, it can be stated that the wheat semolina (0.081 ± 0.005 g) and wheat flour (0.069 ± 0.007 g) substrate used as control gave the best results for the final body weight. In terms of plant raw materials and by-products, sweet potatoes (0.063 ± 0.007) can only be recommended as substrate, while may turnip tuber and may turnip leaf produced significantly lower final body weight results (MT=0.034 ± 0.002 g ; ML= 0.036 ± 0.002 g). The nutrient composition of the larvae was not affected by the substrate, these results confirmed the high protein and fat content reported in the literature. The results with the production parameters were contradicted by the micronutrient content. Larvae reared on may turnip leaf (ML) and tuber (MT) showed the highest values for most of the macro- and microelements (potassium, calcium, magnesium, iron, zinc, copper) tested.
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Nitrogen Content of Hungarian Soils and Nitrogen Fertilization
51-61Views:112For crop production and agricultural production, the most important natural resource is the soil that can optionally renew. Paralelly with this, soil plays a major role in the geological and biological cycle of elements. As a result of the big (geological) and small (biological) element cycles, the elements and combines neccessary for organisms can accumulate in the soil creating suitable living conditions for plants and other organisms. Soil is a heterogenous system both horizontally and vertically, and soil constituents show great variety in all the three dimensions, in addition, most of the parameters can also change between two examination dates. When talking about the factors influencing plant production, one should take into account this variation and heterogenity in time and space. When making fertilization recommendations, these factors should all be considered. In any consultation system, most of the mistakes and errors made are due to the unsatisfying soil testing and the negligence of soil heterogenity. In the practice of fertilization the biggest mistake is the improper soil sampling, then comes the methodical mistake of soil testing, which is followed by the inaccuracy of instrumental analysis and the subjectivity of result evaluation, but the latter two are negligible compared to the others. Under normal, i.e. production conditions, the quantity and distribution of nutrients in the soil are greatly dependent upon the applied technology, the amount and form of the applied natural and artificial fertilizers and the quality of fertilization.
Fertilization recommendations are needed because in the layer which is accessible for plant roots only a part of the nutrient content is available for plants in a specific production cycle. An illustration of this is that though the upper 1 m layer of an average chernozem soil contains more than 5000 kg N, 12000 kg K2O and 1500 kg P2O5 (form of expression mostly used in Hungary), the application of fertilizer doses which are just fractions of these quantities is essential. This is due to the fact that the available amount of the total nutrient content depends from the quality of soil, the environmental factors (the physical and chemical qualities of the soil) and the specific nutrient’s qualities (solubility, adsorption). Knowledge of these processes and the examination of the factors influencing the actual nutrient content are vital for working out a fertilization practice, which does not put more strain on the environment than neccessary.All of the above mentioned should be considered when applying inputs in the fields. In a well-functioning practice that considers the economic and environmental conditions (unfortunately the present production and economic conditions do not enable an appropriate level and degree), three nutrients are supplemented generally (and were supplemented in the last decades): nitrogen, phosphorus, potassium.
Studying the nutrient balance of the Hungarian field production’s last hundred years, we can draw some interesting conclusions.
The nutrient balance became positive for nitrogen and potassium in the second half of the 1960’s, while for phosphorus it was positive from the first half of the 1960’s and this period lasted until the end of the 1980’s.
Neither before the 1960’s, nor since the 1990’s has the amount of nutrients supplemented in a specific year reached the amount of the nutrient uptake of the same year. -
Relation of availability and barley uptake of some potentially toxic elements
7-10Views:84A small-plot microelement load field trial was set up on brown forest clay soil with eight elements (Al, As, Cd, Cr, Cu, Hg, Pb, Zn), on 3 levels each (0/30, 90, 270 kg element ha-1). The soil was treated with soluble salts of elements once at initiation (1994). In the seventh year of the experiment (2001) winter barley was the test plant. The total element content was determined in plant samples (shoot, straw, grain) after microwave digestion using cc.HNO3+cc.H2O2. The element composition of the prepared samples was determined using ICP-MS technique. In the experiment toxic effects of treatments and yield loss could not be observed. Zn and As contents in barely shoots were only moderately increased by increasing microelement loads. Effects of Cr, Cu, Hg, Pb and Al treatments could not be observed. On the other hand, Cd accumulation was significant in the shoot. Cd content was also increased both in straw and grain. Results of this experiment prove that Cd remains mobile in the soil-plant system for a long time. Its accumulation can be observed both in vegetative and reproductive parts of plants without toxic symptoms and yield loss.
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The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
39-44Views:171Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.
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Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:91The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
The study of the fertilizing effect of wheat straw ash in a greenhouse experiment
47-51Views:219The effect of wheat straw ash as a fertlizizer was studied in a pot experiment with an acidic sandy loam soil (pHKCl=4.9) with weak K and P supply. The test plant was ryegrass (Lolium perenne). The treatments were the following: 1. control untreated soil, 2. NPK fertilizer, 3. small dose of ash (1.4 g kg-1), 4 large dose of ash (2.8 g kg-1), 5. small dose ash completed with NP fertilizers. Soil parameters (pHH2O, pHKCl, ammoinum-lactate soluble P, K, 0.01 M CaCl2 soluble PO43--P, K, Mn, Cu, Zn ) and plant parameters (yield, P, K, Ca, Mg, Zn, Mn uptake) were investigated. Based on the analysis of the straw ash sample and the results of pot experiment it can be stated that the wheat straw ash is suitable for the fertilization of the studied soil. The small dose ash completed with NP resulted in the largest yield increment (43%). In order of the treatments the pHKCl changes to: 4.9, 4.8, 5.2, 5.8, 5.1. As the N : P2O5 : K2O ratio is 0 : 1: 3.5 in the wheat straw ash sample, to reach optimal yield ash should be completed with N and P.
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Examination of Zn deficiency on some physiological parameters in case of maize and cucumber seedlings
5-9Views:111Zinc (Zn) is an essential micronutrient needed not only for people, but also crops. Almost half of the world’s cereal crops are deficient in
Zn, leading to poor crop yields. In fact, one-third (33%) of the world's population is at risk of Zn deficiency in rates, ranging from 4% to
73% depending on the given country. Zn deficiency in agricultural soils is also a major global problem affecting both crop yield and quality.
The Zn contents of soils in Hungary are medium or rather small. Generally, the rate of Zn deficiency is higher on sand, sandy loam or soil
types of large organic matter contents. High pH and calcium carbonate contents are the main reasons for the low availability of Zn for
plants (Karimian and Moafpouryan, 1999). It has been reported that the high-concentration application of phosphate fertilisers reduces Zn
availability (Khosgoftarmanesh et al., 2006). Areas with Zn deficiency are particularly extensive in Békés, Fejér and Tolna County in
Hungary, yet these areas feature topsoils of high organic matter contents. Usually, Zn is absorbed strongly in the upper part the soil, and it
has been observed that the uptakeable Zn contents of soil are lower than 1.4 mg kg-1.
Maize is one of the most important crops in Hungary, grown in the largest areas, and belongs to the most sensitive cultures to Zn
deficiency. Zn deficiency can causes serious damage in yield (as large as 80 %), especially in case of maize. On the other hand, Zn
deficiency can also cause serious reduction in the yields of dicots. One of the most important vegetables of canning industry is cucumber,
which is grown all over the world.
In this study, the effects of Zn deficiency have investigated on the growth of shoots and roots, relative and absolute chlorophyll contents,
fresh and dry matter accumulation, total root and shoot lengths, the leaf number and leaf area of test plants in laboratory. Experimental
plants used have been maize (Zea mays L. cv. Reseda sc.) and cucumber (Cucumis sativus L. cv. Delicatess). A monocot and dicot plant have
chosen a to investigate the effects of Zn deficiency, because they have different nutrient uptake mechanism.
It has been observed that the unfavourable effects of Zn deficiency have caused damage in some physiological parameters, and
significantly reduced the growth, chlorophyll contents of monocots and dicots alike. -
Effects of fermented chicken manure products on the N mineralization rate of the soil using the incubation method
199-204Views:199In our study, the effect of fermented and specially added poultry manure products (superabsorbent polymer (SAP), bentonite and Aegis as a mycorrhizal inoculum) were investigated in a short soil incubation experiment – at 60% water capacity level - on sandy soil. Soil samples were collected from two layers of the incubation pots after the second and fourth week to check the status of the tested products and the processes in the soil. The pH and the electric conductivity (EC) of the samples were measured using an electrochemical method, while the ammonium and nitrate content of the samples was determined with a photometric method. Soil pH and EC values slightly were decreased during the experiment. Our results pointed out that the increasing dose of SAP caused lower soil pH. The nitrate content of the soil did not change significantly during the experiment. It was found that the increasing SAP content in the products, due to its cross-linked structural property, protected the nitrate ions from leaching. Our results suggest that applied SAP does not bind the nutrient ions so tightly in its structure that it competes with the plant for uptake.
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Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
134-140Views:107A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
(116 kg ha-1 N) in combination with Microbion UNC bacterial fertilizer (2 kg ha-1) were applied as treatments in a randomized
complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
was chernozem with medium sufficiency level of N and P and poor level of K.
Our main results:
The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
--N and NH4
+-N and also the quantity of soluble organic-N were
almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
--N increased to the greatest
extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
- -N and total-N contents in the plots
treated with ammonium-nitrate, the largest NH4
+-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
treated with urea.
Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
--N, organic-N and total-N compared to the
values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
+-N
content of soil in more cases were higher than that of values with artificial fertilizer treatment.
As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
yielded a decrease in these values compared to the control.
All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
fertilizer increased AL-Mg values in any cases.