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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:357he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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SIM Samples Investigation by Statistical Methods
194-197Views:482The assessment of the present condition of the soil is very important, because the accession of the number of the European Union members is in the near future. This can be the base of the modern agrarian environmental management programme. The assessment must be objective, detailed and analyse the processes in the soil.
Respecting the above causes was decided to create an Environmental Information Monitoring System. This system consists of more parts. One of them is the Soil Information Monitoring System (SIM). This system started to work in 1992.
This system has two functions. Creating and actuation is obligatory from the international contracts, on the other hand the public SIM has very important role in the conservation of the soil.
The SIM territorial measuring grid consists of 1236 measuring points. These points are representatives. The distributions of the points by the types of soil attend the variety of the types of soil of the country.
The investigated elements in 6 types of soil were in our experiment (the group of scandium and the lanthanide series elements). There are 6 elements above the detection limit (Gadolinium, Neodymium, Praseodymium, Scandium, Samarium, Yttrium).
The Neodymium concentration is 2 times higher than the content of Gadolinium and Yttrium.
The Neodymium concentration is 4 times higher than the content of Praseodymium, Scandium and Samarium.
In the case of Dysprosium, Europium, Lutetium, Terbium, Ytterbium the concentrations were below 1 mg/kg. -
Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:576In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. -
Sour cherry seed as an industrial gamma tocopherol source
27-33Views:384In our experiments sour cherry kernels were investigated. The kernels of different sour cherry cultivars were originated from Újfehértói Gyümölcstermesztési Kutató és Szaktanácsadó Nonprofit Közhasznú Kft. Tocopherol profile, oil yields and nutritional value were stood in focus of examinations.
We started processes preparing of sour cherry kernels. After the preparation recovered non-polar fraction was investigated. We worked with formerly developed chromathographic system, which is capable to separate different tocopherol isomers efficiently. We measured concentration of each tocopherol isomers and registered those and their relation in different oil samples.
According to received data, alpha-, gamma- and delta-tocopherol appeared in samples, but beta-tocopherol could not be detected. It became clear that level of gamma-tocopherol is far higher than alfa- or gamma-isomer, it is concentration exceeded 1–1.5 mg ml-1.
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Spatially Continuous GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
56-61Views:374The homogeneity of a study area of 20x20 m used for beetroot production in North-West Hungary was analysed with geo-statistical methods on the basis of measured plant and soil parameters. Based on variogram calculations (Equation 1 and 2), the yield surface showed homogeneity in North-South direction. Considering the results, decrease of sampling distance to 17 m can be suggested. The direction of the variability of yield (Figure 1) could be modelled with a direction variogram based on analysis of the variogram surface. In the study, developed methodological processes are presented for the analysis of spatial relationship between measured production and soil parameters. 5 spatial evaluation methods for yield surface were compared (Table 1). On the basis of the analysed methods, it can be stated that different methods (LP, RBF) should be used when the reasons for locally extreme yields are in focus than in case when the yield surface of the whole area is estimated (IDW, GP). Using adequate parameters the kriging method is applicable for both functions. Similarly to the results of an ordinary Pearson correlation analysis, spatial correlation analysis was shown using soil pH and Cu concentration data. The results of cross variogram analysis (Equation 2) and the North-South direction of the variogram surface showed negative correlation (Figure 3). Based on simulation calculations, decrease of 30% in sampling points resulted in increase of 12% in error for the total sample number considering Cu concentration. The method provides a tool to decrease the cost of sampling and sample analyses of spatially correlating features, and to increase the reliability of spatial estimation using a better sampling strategy with the same sample number.
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Wheat cleaning and milling technologies to reduce DON toxin contamination
89-95Views:752Mycotoxicosis caused by Fusarium fungi holds a huge risk considering economic and food safety issues worldwide. By applying milling technologies, we attempted to reduce the concentrates of DON toxin, as it is the most often found toxin in wheat.
The processes of sieving, aspiration and combination had been used on wheat with high DON toxin concentration. As a next step, grains were sorted using a horizontal cylinder separator, assorted by an optical and a gravity separator, and finally, the products were scoured and ground. The contamination level of the wheat and flour samples were defined by the HPLC-MS method.
Regarding the results, it can be stated that toxin concentration was most effectively reduced by optical separation and scouring, and by applying these milling techniques, food safety can be increased significantly.
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Characterisation of a thermotolerant yeast, Kluyveromyces marxianus CBS712
7-13Views:393Fermentation at high temperature with application of thermotolerant microorganisms is a technological advantage in bioethanol production. Among the yeasts, K. marxianus has outstanding thermotolarance. The industrial application of the IMB3 strain occurs usually at 45C. The final aim of our project is the genetic modification of the K. marxianus CBS712 strain in order to achieve ethanol production at higher temperature than the currently applied. This requires the characterization of the CBS712 strain, with special attention to the determination of the temperature limit of its growth and the amount of the ethanol produced. The temperature limit of growth was 48C in YPD medium. Elevation of the temperature above 45C led to an exponential drop of the cell viability. Ethanol production was tested in shaking flasks, in MYFM medium, under oxigene limited conditions, applying variable concentrations of glucose (12–20%) and different temperatures (45–47 ºC). Preliminary results have revealed that the elevation of glucose concentration increased the amount of ethanol produced. The amount of ethanol (appr. 5%)+ produced at the highest glucose concentration was not different at the tested temperatures (45, 46 and 47 ºC). The observation indicates the potential in raising the thermotolerance of the strain.
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Possibility of oil seeds in feeding dairy cows
67-73Views:499The efforts to modify the fatty acid composition of milk have intensified with health conscious nutrition coming to the forefront.This experiment of ours was designed to investigate to what extent the natural-based feed additives, such as oilseeds, can influence the fatty acid composition of cow’s milk.Further information was gained about feeding of oilseeds in specific amounts to be fitted into the technology of a large-scale dairy farm in practice. The feed supplements were whole, untreated rapeseed and whole, untreated linseed, as part of a total mixed ration. In case of saturated fatty acids when supplementing with whole rapeseed the most significant change was observable in the concentration of the caprylic acid, capric acid, undecylic acid, lauric acid, myristic acid, stearic acid. In case of unsaturated fatty acids the quantity of oleic acid enhanced considerably. When observating the feeding with whole linseed the concentration of many saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, α-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers, as well.
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Comparative analysis of sample preparation methods to determine the concentration of arsenic in soil- and plant-samples
167-170Views:476Arsenic contamination of the fields and groundwater is a global problem. Alföld is the most affected area in Hungary. Irrigation witharsenic contaminated water, and crop production on the contaminated soil can cause a food safety problem, because arsenic is easy taken up by the cell of the plant roots. To prevent this, very important to monitoring the arsenic content of soils and plants. Inductively coupled plasma mass spectrometry (ICP-MS) is a fast, easy method to determined the concentration of minerals in the case of plant and soil samples The analytical methods can give reliable, results if the analytical process, including the sample preparation method, is the best. The objective of this study was to compare 3 type of sample preparation method which was dry ashing, wet digestion in open system, and microwave digestion. As a result of our experiement shows the microwave digestion is the appropriate method to determined the arsenic content of soil samples. In the case of plant samples we can use wet digestion in open system or microwave digestion as a samle preparation method.
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Examination of the efficacy of different fungicides against Macrophomina phaseolina and Sclerotinia sclerotiorum in laboratory conditions
21-24Views:584Macrophomina phaseolina and Sclerotinia sclerotiorum are two significant fungal pathogens of sunflower. M. phaseolina causes charcoal rot and ashy stem blight in several important crop species. Sclerotinia sclerotiorum causes white mold disease which can occur as middle stalk rot, head rot and premature plant death. Due to the wide host range of the two pathogens and their survival structures, crop rotation cannot provide sufficient protection against them. In our experiment, we selected two fungicides, Mirage and Prosaro, which are widely used in practice, and we tested their efficacy against the two pathogens. The efficiency of these fungicides was tested at a concentration of 10; 20; 50; 100 and 500 ppm. The Prosaro totally inhibited the mycelial growth of both pathogens at a concentration of 50 ppm, 100 ppm and 500 ppm. The Mirage caused total mycelial growth inhibition in all treatments against both pathogens.
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Effect of molybdenum treatment on uptake of plant and soil molybdenum content in a field experiment
117-122Views:580Molybdenum is not a well-known microelement, but being a constituent of several important cellular enzymes it is an essential microelement. Molybdenum occurs in all foods, but at very low levels. There does not appear to be any particular foods or types of foods, which in the absence of extrinsic factors, naturally have high levels of molybdenum. However, environmental pollution, from natural or anthropogenic sources, can lead to high level of the metal in plants.
Our study is based on the long-term field experiments of Nagyhörcsök, where different levels of soil contamination conditions are simulated. Soil and plant samples were collected from the experiment station to study the behaviour of molybdenum: total concentration, available concentration, leaching, transformation, uptake by and transport within the plants, accumulation in different organs, phytotoxicity and effects on the quantity and quality of the crop. In this work we present the results of maize and peas and the soil samples related to them.
According to our data molybdenum is leaching from the topsoil at a medium rate and it appears in the deeper layers. In the case of plant samples we found that molybdenum level in the straw is many times higher than that is in the grain, so molybdenum accumulates in the vegetative organs of the plant. The data also show differences in the molybdenum-uptake of cereals and Fabaceae (or Leguminosae). -
The effect of collecting area on the element content of Hungarian acacia honeys
129-138Views:555Six macroelements and twelve microelements were identified in thirty-six Hungarian acacia honeys collected from ten counties by inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). One-Way ANOVA (LSD and Dunnett T3 test) and linear discriminant analysis (LDA) were used to determine the statistically verified differences among the honey samples with different geographical origin.
Significant differences were established among the samples from different counties in Na, P, S, Fe, Ni, Cu and Sr concentrations. Based on the macroelement content of honeys, the separation of samples with different geographical origin was not successful because the percent of correctly categorised cases was only 64.9%. However, examining the As, B, Ba, Cu, Fe Mn, Ni and Sr concentration, the separation of different groups was convincing since the percent of correctly classified cases was 97.2%. Thus, the examination of microelement concentration may be able to determine the geographical origin of acacia honeys.
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Following-up organic pollutants in the course of producing foods
244-248Views:557We analysed the raw materials in various food products, including additives, carrier solvents and end product for several selected organic pollutants. We analysed these food products for the type of chlorinated hydrocarbon pesticides, polychlorinated biphenyls and other currently used pesticides.
We analysed three product categories: bakery products, canned mushroom and meat products. The analysis of the bakery products and of the canned mushroom did not conclude in positive findings, which means that these products contained the agents given during the production of the product and the environmental pollutants in undetectable low concentration.
However, the results for meat and fish products indicate that polychlorinated compounds are persistent and are of a lipophyl character, as some of these components have been detected in these food products, the concentration of the PCBs were detected in the range of 9.8-24 μg/kg. -
Toxic elemental content of Hungarian blaufränkisch wines
87-90Views:577Toxic elemental contents are one of the food safety risks in wines. Therefore International Organization of Vine and Wine (O.I.V.) defined the limit of some elements in it. Thirty Hungarian blaufränkisch wines were analysed by ICP-MS in order to determine the concentration of toxic elements. All wines are passed by the O.I.V limits to toxic element content (Cu, Zn, Cd, Pb) according to analysis. Copper is the only outlier in some samples, but they also did not overstep the limit. In the case of zinc the maximum is approximately fourth, in case of cadmium and lead the highest concentration is less than tenth and sixth of the O.I.V. limit, respectively.
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The effect of the preparation method on the physical and chemical characteristics of propolis tinctures
163-168Views:715The effect of the preparation method was examined with regards to the physical and chemical characteristics of the propolis tincture, namely the extraction time and the ethanol content of the extraction solvent to the dry matter, polyphenol, flavonoid, phosphorus, calcium and copper concentration, respectively. The dry matter, the polyphenol and the flavonoid content were the lowest in the water extract of the propolis; however, significant increase was noticed depending on the extraction time. Significantly higher concentrations were found in 50 V/V% tinctures. The highest dry matter and flavonoid contents were analysed in 100 V/V% tinctures, whereas highest polyphenol content was found in 80 V/V% tinctures. However, the differences were not significant in several cases between latter tinctures. Moreover, the increase was not determined in some cases depending on the extraction time. Phosphorus and calcium concentrations were decreased depending on the increasing ethanol content, whereas copper concentration was increased up to 80 V/V%. Higher increase was found in the case of 0 and 50 V/V% extracts than in 80 and 100 V/V% tinctures. Moreover, in latter cases, no significant differences were found on several occasions, depending on the extraction time. There was no connection between the flavonoids and the calcium as well as the phosphorus content, whereas flavonoids may be made complex with copper. However, the amount of the possible complex was negligible.
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The effect of irrigation on the yield and quality of maize (Zea mays L.) hybrids
143-147Views:358In this study, the effect of water supply on the quality and productivity of different maize hybrids was observed.
Maize production is influenced by many agro-technical factors such as nutrient supply, plant density, environmental factors, water supply and temperature. Good soil quality and adequate technology significantly reduce the unfavourable effect of crop year. The impact of fertilization, crop rotation, irrigation and plant density on maize yield was greatly affected by crop year and crop rotation. The main constituent (69–75% dry matter concentration) of maize seed is starch, however, its increase can be achieved only to a limited extent, as increasing the starch content will reduce other parameters, especially protein content. Significant nutrient content improvements can be achieved by appropriate hybrid-site connection and the use of adequate technology. Protein content reduction with optimal water supply can be positively influenced by the appropriate nutrient supply for the hybrid.
The experiments were carried out at the Látókép Experimental Site of Crop Production on calcareous chernozem soil. The weather of the examined year, which was partly favorable for maize and partly unfavourable in other respects, was also reflected in the development of maize and yields achieved.
In 2019, we were examining the hybrids of Kamaria (FAO 370), P 9903 (FAO 390), DKC 4351 (FAO 370) and KWS Kamparis (FAO 350–400). Due to the dry soil condition, sowing was delayed, however, the hybrids emerged ideally because rain arrived soon after sowing, which facilitated initial development. Young plants evolved rapidly and dynamically in the case of all hybrids. In terms of heat-demanding bread, the month of May was unfavourable to temperatures below the annual average, but the higher amount rainfall helped the development. However, due to the drier period in early July, 25–25 mm of irrigation water was applied to half of the experimental areas on July 1 and 15.
The aim of our research was to determine the best population density for hybrids under favourable soil conditions (calcareous chernozem soil). In both irrigated and dry conditions, a crop density of 75,000 crops were the most favourable for the Kamaria hybrid. The P9903 hybrid in the case of a crop density of 85 crops proved to be ideal and the DKC4351 had an optimal population density of 95,000.
However, it should be taken into consideration that, in the case of soils with poor water management, the drought sensitivity of the crop stand may increase at a population density of 95,000.
As a result of irrigation, yield increased and the difference between the examined plant numbers decreased. The yield growth was relatively moderate (341 kg ha-1 – 1053 kg ha-1), which makes the economicalness of irrigation doubtful in the given year.
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Coherence and connection between the good pond culture practice and the environment conscious management
60-73Views:188According to the data indicating the decline and restructuring during the past decade, as well as the trend in the European Union member states, it can be expected that the role of traditional agriculture and fish production in direct rural employment decrease further. This also values those strategic directions for restructuring that will lead fishculture from quantity driven to quality production along with sustainable development (i.e. environmental conscious production) and multifunctional farming. This way the economic and social tensions caused by the concentration of the production and labour output can be mitigated.
It is laid in the 1257/1999 Act on rural Development that farmers that enrol the agri-environmental scheme should follow the “Good Agricultural Practice” on the whole managed area. In case of agri-environmental schemes this is a precondition for which no grants are given.
The adaptation of “Good Agricultural Practice” in fishproduction,where it is called: “Good Pond Culture Practice” is considered important on the basis of the above mentioned. This programme is undertaken in co-operation with the Research Institute for Aquaculture, Fisheries and Irrigation, University of Debrecen, Faculty of Agricultural Economics and Rural Development and the Association of Hungarian Fish Farmers and Product Council.
The European Commission proposed the formulation of the European Fisheries and Aquaculture Fund (EFAF) for the period 2007-20013, which will replace the Financial Instruments for Fisheries Guidance (FIFG), but it also consists of several new elements and will be working differently, too. According to the proposal the budget for the Fund will be nearly 5 billion EUR (4963 million EUR). The development level of aquaculture and fisheries and the social and economic significance in the given member state will be considered when distributing the Fund between the Member States.
According to the plan the Fund is organised along five priority axes, of which the most important for the Hungarian fisheries sector is No. II: Aquaculture and the processing and marketing of aquaculture and fisheries products. The main measure areas are the followings:
1. investment support for aquaculture;
2. support for aquatic-environmental schemes;
3. environmental- and animal health issues;
4. investments in processing and marketing
In case of accessing support under measure area No. 2 farms are obliged to meet the requirements of the scheme beyond the “good management practice” for 5 years, which is to be supervised by the approved body of the Member State. For this reason our work is considered to be substantial.
Approval of the application of “Good Pond Culture Practice” is based on two elements: first the prevailing environmental and nature conservation regulations, as well as the list of controllable conditions in the new agri-environmental agreements are to be met. “Good Pond Culture Practice” are to be conducted on the whole farm area. Its main elements are:
- nutrition management,
- feeding,
- pond maintenance,
- stocking,
- harvesting,
- animal welfare (storage and over-wintering). -
Changes in the Sulphur Content of Winter Wheat in a Field Experiment
85-88Views:201The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring. -
Testing a biological active plant extract’s antifungal effect against soil fungi
247-252Views:241In Hungary today is about 5 million hectares of agricultural land contaminated with ragweed. The ragweed problem a year is about 60 billion HUF to be paid, of which 30 billion are used to reduce the agricultural damage. Experiments with ragweed pollen has mainly been carried out in connection with terms of allergy. The other biochemical experiments and studies with this plant, have so far been the scientific horizons of public life, boosted the edge. We wanted to demonstrate that the ragweed, which is a weed, containsbiological active (for example: antifungal) compounds. For our experiments in the previous cycle of flowering, plants were collected manually, with its roots and with each plant part. The extraction of the substance from dry plant – meal was carried out using appropriate solvents. The biological activity of ragweed-extracts were tested against fungi isolated from soils and meadow with different mode of cultivation. Our results suggest that ragweed contains biologically active substances, which inhibit the growth of fungi, depending on the concentration of active ingredients of the plant.
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Assessment and comparison of selenium-enriched maize with sodium selenite and sodium selenate
11-15Views:550Selenium is an element of environmental interest owing to the narrow range between its nutritionally required and toxic concentrations in many organisms. Its mobility and bioavailability differ greatly depending on individual Se species. In this regard, in present study, the uptake and distribution of Se, the changes in Se content, and the effects of different concentration of Se in two forms of sodium selenite and sodium selenate on maize plants were measured in nutrient solution experiments to clarify their response to the two forms of Se. The results revealed that the Se content in shoots and roots of maize plants significantly increased as the Se level increased. Two Se forms behaved differently and the effects of toxic damage in samples which had been treated with selenite were much more than in the selenate treatments.
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The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
135-140Views:352Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.
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A tárolási feltételek hatása a kukorica Fusarium fertőzöttségére és toxin szennyezettségére
28-32Views:1261Corn samples harvested in 1997, 1998 and 1999 from different soil types were stored at different conditions (temperature, moisture content, state of kernels) for six months. The Fusarium contamination was examined by plate dilution method and the amount of mycotoxins (F-2, T-2, HT-2, DON, DAS) were determined applying HPLC method immediately after harvesting and in the third and sixth month of storage. The aim of our study was to find correlation between the ecological factors, storage conditions and the examined parameters mentioned above, as well as to prove them statistically. According to the examinations carried out after harvesting we could state that the soil type had no effect on the parameters. Analysing the effect of the years we found considerable differences. The Fusarium infection of corn samples in 1998, while the toxin contamination in 1999 was the highest. The results of storage experiment show that year (number of microscopic fungi, F-2, T-2, HT-2, DAS and total toxin content) and moisture content of kernels (F-2, T-2, and total toxin content) have a significant effect on the examined parameters. We could prove the effect of temperature on the T-2 content (samples with natural moisture content) and DON content (samples with 14% moisture content). Higher values were found at higher temperature storage. The ratio of damaged kernels influenced the DON concentration in the non- wetted samples and the number of microscopic fungi in the wetted samples.
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Effect of ethephon levels and amino acids on the growth characteristics of oat crop
57-61Views:261A field experiment was conducted during the 2021-2022 season in an agricultural field in Basra Governorate. The aim was to study the effect of spraying ethephon and amino acids on the growth characteristics of oats (Avena sativa L.) Shifa cultivar. The experiment involved three levels of ethephon (0, 0.240, and 0.600 kg ha-1), represented by the symbols E0, E1, and E2 respectively, and two spray levels of amino acids (0 and 5 ml L-1), represented by the symbols A0 and A1. A factorial experiment was applied using the R.C.B.D (randomized complete block design) in a split plots arrangement with three replications. The results revealed significant differences in the levels of ethephon for most of the studied characteristics. The level of 0.240 kg ha-1 (E1) was superior in terms of chlorophyll content, crop growth rate, and the number of tillers, with increases of 39.07%, 39.26%, and 16.36%, respectively, compared to the control treatment (E0). Regarding the amino acids treatments, spraying at a concentration of 5 ml L-1 (A1) demonstrated significant superiority, resulting in the highest plant height, flag leaf area, chlorophyll content, and crop growth rate. This study concludes that amino acids play a crucial role in plant growth, and the combination of 5 ml L-1 amino acids with 0.240 kg ha-1 of ethephon significantly increased chlorophyll content and crop growth rate.
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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:713Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:426The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally.