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A review on the trend of livestock breeds in Laos
227-237Views:613When the livestock sector is considered an essential role in farmers' livelihood, and local breeds are still the critical component of animal breeding in Laos. Thus, there is a need to review the previous, current situation and prospects for Laos' livestock production. It aimed to study the different traits among the existing dominant local and improved livestock breeds (cattle, pig, and goat) and a more in-depth study on the livestock sector's previous evolving and prospects. Our findings revealed that the general trend of livestock population in Laos increased year-by-year for over 45 years, except for 1995 to 2000. It was decreased by almost 22% due to the new national strategy on economic revolution. The first foreign breeds were introduced into Laos, i.e., Large White, Landrace, and Duroc in 1980, Red Sindhi bulls in 1998, the frozen semen of Red Brahman 2003, and Bach Thao goats between 2001 to 2003. Unfortunately, many data are still not precise for introducing other foreign breeds like Boer goats, Thai Grey and Red Brahman. There were many missing data on the improvement of livestock breeds in Laos, including the local and improved breeds' reproductive and productive performance, which needs more study and research. We conclude that the semi-complex farm operation should be applied to improve livestock breeding and their productions in Laos.
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Effect of G2548A polymorphism in the leptin gene on the BMI level in human population
5-10Views:246The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.
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Industrial Utilization of Grasses
131-134Views:135The Agricultural Research and Development Institute P.U.C., Szarvas – realizing the importance of using the biomass in a number of ways – was one of the first in Europe, who began the breeding of grasses suitable for industrial utilization. The possible areas of using the energygrass – which could be called industrial grass – are the following:
– Energetical use
– Paper industry use
– Utilization as an industrial fibrematerial
– Use for feeding
The aim of the breeding programme: developing the kinds of grass, which yields big drymaterial mass and suitable for industrial utilization. These kind of grass, taking soil-utilization, economicality and environmental protection, offer and ensure new marketing perspectives and employment facilities for the disadvantageous areas. -
Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:250The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
‘Kindergarten’ keeping-system in farrowing house: effect the socialization of piglets on weight performances, fecal cortisol metabolite level and post-weaning behavior
167-174Views:371This study aimed to examine whether litters’ let-together system before weaning (‘kindergarten-system’) has any stress effect and effect on post-weaning behavior, respectively. One week before weaning piglets were socialized by removing four adjacent farrowing crate walls. After weaning, piglets from the same experimental groups were housed in the same rearing crates. The piglet’s body weight was measured at birth than weekly till the 7th life-week. Fecal cortisol metabolites were measured for evaluation of the adrenocortical activity. Personal observation and evaluation were carried out for behavioral analyses after weaning. There were no significant differences in weight development and cortisol metabolite levels between the control and experimental groups. Behavioral analysis showed that pigs grow up in a socialized system, rapidly evolve ranking in the rearing crates, and the self-maintenance and social behavior were more pronounced. In control groups, the activity involving movement (exploring, walking, and running) was much more decisive. Recent work suggested the beneficial effect of the ‘kindergarten’ system in the farrowing house in animal welfare aspects.
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Using phenotypic correlations in the breeding evaluation of Hungarian Sport Horse mares
38-43Views:294The aim of this paper is to analyse phenotypic relationships between self performance test (SPT) parameters of Hungarian Sport Horse mares. Establishing these relationships may contribute to develop a breeding value estimation model. Data were given by the Association of Hungarian Sport Horse Breeders.
The analysis is based on the SPT results of 3 and 4 year old mares from 1993 to 2004. Our aim is to determine practically explainable correlations among conformational traits (type, head, neck, saddle region, frame, forelegs, hind legs, regularity of movement, impulsion and elasticity of movement, overall impression); free jumping performance (jumping style, jumping ability, observation during training) and movement analysis traits (walk, trot, canter, overall impression, test rider’s score). A further aim is to determine correlations between free jumping performance and movement analysis traits. -
Sporthorse performance testing in eventing by own and progeny performance
49-56Views:356The aim of the study was to evaluate the Hungarian Sporthorse population based on eventing competition performance. The database contained the results of 792 horses and 449 riders between 2000 and 2006. The eventing results were gathered from Hungary and other European countries. Blom transformed ranks were used to evaluate the sport performance.Three models were fitted to the Blom scores. Evaluating all the competition categories at the same time weighted Blom scores were used according to the difficulty of the category. The linear mixed model included fixed effects for age, sex, breeder, owner, location, year; and random effects for animal and rider. Horses from the database were judged by their own performance, and stallions were investigated by performance of their progenies on the basis of descriptive statistics of Blom scores and weighted Blom scores. Breeding values of eventing performance were predicted. To improve the reliability of breeding values, more progenies should be
used in eventing competitions. -
Evaluation of egg quality parameters of two Hungarian ostrich populations
51-57Views:416The aim of our study was to evaluate the quality parameters, porosity and weight loss of eggs deriving from the two most significant ostrich farms in Hungary. Quality parameters included weight, length, width, shape index, egg volume, surface area, circumference and shell volume. The effect of storage conditions in both farms and the incubation technology on egg weight loss in farm “A” were also examined. The research objective was to impart a comprehensive knowledge on egg quality parameters of the main ostrich populations in Hungary and to compare the trios and the farms with each other and the international literature. We could reveal significant differences between trios in all egg quality traits. In conclusion, the shorter and the narrower the eggs were, the more spherical shape they had. Narrower eggs showed smaller surface area, volume, circumference and shell volume and vice versa. Eggs from farm “B” indicated significantly greater width, shape index, surface area, circumference and shell volume than farm “A”. A significant difference was observed in weight loss during storage between the farms. Weight loss in farm “A” was a multiple of farm “B”. In farm “B” there was a weak, positive correlation between storage period and weight loss (r=0.22, P≤0.05), in farm “A” it was not significant (P=0.52). There was no relationship between the initial egg weight and weight loss either in farm “A” or farm “B” (P=0,21, P=0,69). A slight positive correlation could be noted between egg porosity and weight loss (r=0.24, P≤0.05). Pores count presented here was less than the international results. Poultry eggs contain the most pores at the blunt end, less via the equator and the least at the pointed end. In ostrich egg we found more pores via the equator against the blunt end. To draw more precise conclusions, further investigation should be carried out on porosity. Considering the fact that the length of storage period and the weight loss during incubation are in strict correlation with hatchability, we intend to extend our research aims to these traits.
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Lambing Frequency in the Ile de France, Suffolk and Babolna TETRA Stocks of Babolna c.o., Szendrő
29-32Views:513Lambing frequency of three different genotypes was evaluated in the breeding stock of Babolna c.o. Szendrő. Ile de France, Suffolk and Babolna TETRA breeding ewes gave the data for the further evaluation. The main aim of this research was to find out if there is any significant effect of selected parameters (registration class, lambing season, lambing type and the years) on the lambing frequency, the period between consecutive lambings. Production parameters of 9,471 Ile de France, 1,244 Suffolk and 7,618 Babolna TETRA ewes were collected and processed. The analysis covered the period between the years 1989-1999. It was concluded that not all of the evaluated parameters had a significant effect on lambing frequency. Not all of the genotypes showed significant differences, according to the examined parameters.
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Origin, history and utilization possibilities of Carpathian Braunvieh at Hungary
15-20Views:441The Carpathian Braunvieh was established by the cross-breeding of Braunvieh and the small dun mountain breeds of the Carpathian basin. It has been breeding in Hungary about 100 years before and still living in Transcarpathia and Transylvania, but only in very mixed populations. Since it was a characteristic native cattle breed of the country, it should be retain for future generations and re-establish in Hungary. Our primary aim is to preserve the breed’s genetic resources and - if appropriate - to acclaim it as an indigenous breed. If there are no longer residuals of the old type Carpathian Braunvieh, it is still worth to preserve as a local variety, because of its many favourable features. Above all, it’s our duty to maintain the breed, because neither Transcarpathia nor Transylvania has a breeding programme for it.
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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:201The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Ovarian performance of Barbados Blackbelly and Dorper crossbred ewes in the mirror of body conditions
23-26Views:181The aim of examination was to detect the puberty and to follow the ovarian cycle of Barbados Blackbelly×Dorper ewes in connection with their body conditions and the effects of meteorological parameters. The blood samples had been taken from 17th of November 2008 untill 08th of June 2009 for analyzing the progesteron concentrations, establishing whether they showed perpetual ovarian performance or not. The end of examination
was timed due to releasing the ram in middle of May 2009.
The daily avarage bodyweight-growth is well featured by the bodyweight tendence. The body conditions were evaluated by ultrasound tissue examination.
During the full length of the examination the avarage daily temperature, the avarage humidity level and also the number of daily sunlighted hours were served by the Agrometeorological Observatory of the University of Debrecen.
The bodyweight tendence and the meteorological parameters were correlated to the breeding season of ewes. In conclusion the bodyweights and their tendences at the young age and the body condition parameters measured at certain anatomical areas can influence the length of the breeding season of the ewes. However the number of daily avarage sunlighted hours, the daily avarage temperature and also the humidity did not effect the cyclic ovarian perfomance of the Barbados Blackbelly×Dorper ewes in Hungary. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:193The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Gene Bank Developed by Induced Mutation for Selection
45-49Views:198Heterosis breeding in maize caused gene erosion by using uniform inbred lines. In order to strengthen the genetic base, we established a gene bank containing lines with broad genetic variability, resistance and adaptability. The maize gene bank is a result of our work in the past two decades.
The gene bank originated from treatments of maize seeds of hybrids and inbred lines with fast neutrons. The 1500 maize lines have great genetic variability which can be exploited after strict assessment and selection. As a result of the past several years, P 26, P 61 and P 62 lines have been released after DUS investigation in 2001. -
The methodology of the Hooded Crow’s (Corvus cornix L.) colour ringing and the previous results
43-48Views:295The Hooded Crow (CorvuscornixL.) have moved in several Hungarian cities in the last few decades. It is breeding in Debrecen since 1959, and nowdays it’s presence can be detected at all points of the city, it is an permanent breeding species of the bird fauna. Our knowledge about the nature of urban races, including motion patterns, area fidelity is sufficiently incomplete. The aim of our research is to answer these questions by using our colour-ring program. In this study we present in details the methodology of Hooded Crow’s colour-ring program in urban area, and we also report our previous
achievements. -
The effect of feeding different glycerol sources on the performance of lactating sows
99-103Views:248Glycerol is a by-product of the biodiesel industry and it might be a good alternative to moderate the energy deficiency of sows during the lactation period. Preliminary experiments were carried out to test the effect of a powder, solid based “food grade” glycerol source with 72.9% glycerol content (Trial 1) and a liquid “feed grade” glycerol source with 86% glycerol content (Trial 2) on the performance of lactating sows and their litters. Trial 1 was conducted with 5 Hungarian Large White×Hungarian Landrace sows/treatment (313±24.9 kg) and Trial 2 with 12–12 DanAvl (323±17.0 kg) sows and their litters/treatment. Neither the solid, powder based glycerol (Trial 1), nor the liquid glycerol source (Trial 2) had significant effect on the feed intake, reduction in live weight and back-fat thickness, and weaning-tooestrus interval (p>0.05) of lactating sows. In Trial 2, on the 14th, 21st and 27th days of lactation the milk samples were collected and it was found that 50 kg/t glycerol decreased the protein content of milk samples (p<0.05). Glycerol supplementation had no effect on dry matter, fat, lactose content of milk samples (p>0.05). In Trial 2, no significant difference was found between control and experimental sow groups in triglyceride concentration of blood samples and in the activity of liver enzymes (ALT, AST, GGT; p>0.05), but the concentration of plasma glucose and cholesterol increased tendentiously (p<0.10).
Based on our preliminary results, it can be concluded that additional dose trials are needed to perform in order to study the effect of glycerol supplement on milk production and on metabolic processes of lactating sows.
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Molecular genetic investigations for increasing the production results of farm animals
92-96Views:145The author analysed the publications related to molecular genetics presented at the 51st annual meeting of the European Association for Animal Production. Her aim was to make the latest molecular genetic results known and to emphasise that this discipline plays an increasingly important role in research and the practical fields of animal breeding and production.
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Investigation of genetic diversity in irradiated maize lines and its relation to hybrid performance
20-26Views:568Knowledge of genetic diversity among available parental lines is fundamental for successful hybrid maize breeding. The aims of this study were to estimate (1) genetic similarity (GS) and genetic distance (GD) (based on Jaccard index) in four maize inbreed lines; (2) to classify the lines according to their GD and GS; (3) to determine hybrid performance based on GD and heterosis for yield ability in 4x4 full diallel system. We used morphological description and AFLP (amplified fragment length polymorphisms) for estimation genetic polymorphism in four maize inbred lines. We estimated the applicability of genetic similarity in SC and reciproc hybrids for prediction of their performance.
Three primer combinations were used to obtain AFLP markers, producing 207 bands, 70 of whit were polimorphic. The dendogram based on genetic similarities (GS) and genetic distance (GD) and morphological description separated four inbred lines into well-defined groups. Morphological description just with AFLP analysis showed reliable results. In view of genetic distance, the UDL 1 line and their linear and reciprocal crosses showed significant heterosis effect, which was confirmed by heterosis calculation based on grain yield. -
Analyzing the Efficiency of Dairy Farms by Using the Method of Data Envelopment Analysis (DEA)
17-21Views:267In Hungary the dairy sector is in a long-term critical period, the stock has been in constant decline. The consumption of milk and dairy products in Hungary is slightly rising compared to the world tendency, and it is fallen behind the level in 1990. The milk consumption per capita in 2006 was with 75 liters less than the EU-15 average. Dairy enterprise is a very risky activity: the profitability of the enterprise is affected by the fluctuation of feed and animal health products prices from the side of inputs, and by the fluctuation of end-product prices. Under these circumstances it is vital for the
cattle breeders, in order to survive, to harness the reserves in the breeding as effectively as possible. In our research we made a multi-faceted efficiency analysis of an agricultural holding’s three dairy farms. The chosen method for the analysis was Data Envelopment Analysis (DEA). The
selection of the method is justified by the fact that there is not such a reliable database by which we could define production functions, and that DEA makes possible to manage several inputs and outputs, i.e. multiple decision problems, simultaneously. By using DEA the sources that causes shortfalls can be identified, analyzed and quantified on farms that does not operate efficiently, thus it can help the corporate decision support successfully. In the model inputs are the cost data per one liter milk – feed, medicinal product use, logistic costs -, and the main parameters
concerning the keeping and rearing. Outputs are indicators concerning milk production, milk quality and others. We prepared the model in MS Excel, the linear programming model series were programmed by Visual Basic. After solving the model, in light of the shadow prices we can determine why either of the farms is not efficient. -
Some Practical and Biotechnological Methods for Improving Reproduction Traits in Sheep
15-20Views:232However, reproduction in sheep is seasonal, many breeds of sheep are able to mate not only in autumn, but out-of-season as well. The main factor determining seasonality is the photoperiod, but other factors can influence reproductive pattern, such as genetics, management practices and social cues. The fertility of spring and early summer breeding is usually lower; this imposes the need for alternative methods (e.g. hormonal treatments, biotechnological practice), to increase the conception rate.
The author summarize the main practical techniques and biotechnological methods for controlling reproduction completed with some own experimental results in connection with different topics. -
Effect of floor type and bedding system on sows lifespan
155-160Views:271The paper studies two nucleus pig farms in the Great Plain region of Hungary which have same management, same feeding system but different breeding technology (Farm A had solid floor with straw and Farm B had slatted floor without straw). The genetics of sows were crossbred Dutch Large White and Dutch Landrace. The comparative examination was based on the causes of culling according to the different floor type. To the analysis of culling reasons was applied the methods of survival analysis.
In the analysis there were used the Kaplan-Meier method and the Cox Proportional Hazard model to the estimate the difference between the farms. The results of survival analysis showed significant difference (p<0.05) between the lifespan of sows that caused the difference between the survival probabilities of sows culled due to lameness. Furthermore there was showed that the sows kept on solid floor with straw have stayed in production longer than on slatted floor without straw. The hazard ratio was 1.434 that means that the culling due to lameness is 1.434 times higher by the slatted floor without straw facing by solid floor with straw.
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Effect of different n-6/n-3 fatty acid proportion oil sources on reproduction performance and fatty acid profile of milk in modern genotype sows - Pleminary results: Preliminary results
121-128Views:266This study was conducted to investigate the effects of supplemental n-6 and n-3 fatty acids on sow’s milk fatty acid composition during the lactation period and on reproductive efficiency of sows in the subsequent gestation period. Data were collected on a total of 213 DanBred sows (108 control and 105 experimental) representing parity of 2–7, respectively. Control and experimental sows were placed in the same housing conditions during lactation and gestation period. Control group received 6.3 g of sunflower oil (SO) per kg feed as n-6 fatty acid supplementation, whilst experimental animals received the same amount of fish oil (FO) as n-3 fatty acid source. Diets were corn meal-extracted soybean meal based. The experiment was conducted in one replication as being a part of a longer and more comprehensive trial. It was found that the consumed long-chain polyunsaturated fatty acids (LC-PUFAs) appeared in the sow’s milk and changed its fatty acid profile. With this alteration, the n-6/n-3 ratio of experimental (FO) sows’ milk were narrower than in SO group (SO: 13.82 vs. FO: 5.89). The benefits of n-3 fatty acids supplementation were evident for the subsequent reproduction cycle, when experimental sows heated more reliable and earlier than control (weaning to oestrus interval: SO: 5.86 vs. FO: 4.48 days). Only 2.33% of experimental sows (FO) did not heat within 7 days after weaning, but this was 12.36% in the control group (SO). The present study requires further research to evaluate the effect of n-3 fatty acids on maintenance of pregnancy and improved subsequent litter size.
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:225Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:167Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Examination of microsatellite markers of Dorper sheep breed
57-61Views:426Number of not woolly and molty sheep exceeds 60 million throughout the world. Their numbers and their importance is growing, still they have appeared in the past two decades all over in North-America, Australia, New-Zealand and also in Europe. The South African Dorper has been a pioneer among them in Hungary. It was introduced in 2006 in the country. The Dorper sheep is the second largest breed in South Africa, which was developed from the crossing of Dorset Horn and the Blackhead Persian. The aim of the EU Member States in terms of this specific breed is increasing the small populations, improving the productive qualities, in addition to this avoiding inbreeding. However, finding appropriate breeding stock is difficult due to the small size of available populations and also to the suspected common of origin. With the help of various molecular genetic methods we could get a total view of the genetic background of these flocks. Nowadays the most commonly known and used genetic markers are microsatellites, because their applications give fast, accurate and easily reproducible results. There is no specific descriptive information on the genetic background of Dorper populations in the various EU countries , also regarding diversity between populations. Therefore in our work we want to optimize the conditions of applicability of 31 selected microsatellite reactions as a first step of mapping the entire genetic background of the different EU Dorper populations.