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Performance of sweet cherry cultivars grafted on Colt rootstock
7-10.Views:981In this paper growing characteristics and fruit bearing parameters of ‘Lapins’, ‘Kordia’, ‘Sweetheart’ and ‘Regina’ sweet cherry cultivars grafted on vigorous Colt rootstock were evaluated at the University of Debrecen, Pallag Experimental Station. Based on our data five years old trees can be described with homogenous strong growing, but very week yielding (2.1-3.1 kg/tree), as while fruit size varies between 26.2 and 27.2 mm. Producing the examined cultivars on Colt rootstock with high plant density requires higher attention and more interventions (root pruning, sawing the trunk) during the technology.
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Vegetative and micropropagation potential of Piper guineense (Schumach and Thonn)
29-36Views:388The continuous loss of forest plants due to deforestation, and the increasing demand for Piper guineense because of its medicinal and food value, has put a permanent pressure on its population in the wild where it is collected. A method for conservation and mass propagation is therefore required. This research was undertaken to determine the optimal concentration of auxin needed for vegetative propagation and to investigate the potential of Piper guineense for micropropagation. The auxin optimization study of vegetative propagation was based on the use of two-nodal stem cuttings treated with five different concentrations of indole-butyric acid (IBA). Growth parameters such as the number of sprouted, rooted and survived cuttings among others were determined. To investigate the potential of Piper guineense for micropropagation, nodal explants were subjected to different sterilizing treatments using ethanol, NaOCl, mancozeb, streptomycin and Plant Preservative Mixture (PPM). The effect of plant growth regulators (PGRs) was tested on sterilized nodal explants using full strength Murashige and Skoog (MS) hormone-free media alone as control and MS media supplemented with PGRs (BA, NAA and KIN) at different concentrations and combinations. Significant differences were observed across the treatments for all growth parameters measured. However, 2000 ppm IBA significantly (p<0.05) influenced sprouting and rooting of the stem cuttings. Piper guineense explants have deep tissue contaminants, which cannot be eradicated by surface sterilization alone except double sterilization using PPM. On control media, neither shoot nor root response was observed while the highest percentage of induced roots was obtained from explants cultured on MS +1 mg/L BA + 0.25 mg/L NAA. Shoot induction was only achieved when BA was used alone and when subcultured on media supplemented with NAA, which generated roots.
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Dr. Ottó Orsós, the forgotten Hungarian pioneer in plant tissue culture
9-13.Views:388The knowledge of tissue culture deserves attention in respect of understanding the development of universal biology. This study intends to contribute to the past of the plant tissue culture by such data of the history of science which have been unprocessed so far. It seems that the life-work of the Hungarian biologist, Dr. Ottó Orsós is a missing and essential link between those early plant hormone researchers and the representatives of the pioneers of tissue culture schools who have contributed substantially to the development of the modern in vitro plant morphogenesis and plant cell biology. Orsós cultured kohlrabi tuber cubes on White culture medium in a sterile manner. This way, he could efficiently direct the in vitro morphogenesis of the kohlrabi, the regeneration of its shoot and root, and the formation and steps to subculture of pure callus tissues in 1938. He supported the correctness of its statements by means of detailed anatomical examinations. Orsós successfully rooted and aclimatized complete regenerated plants. We may as well call the above system — in remembrance of the creators of the original concept — "Haberlandt-Orsós model". Between the publishing of his main paper in 1938 and 2003, a period of 65 years has lapsed. On the occasion of this anniversary, we bow before this forgotten pioneer.
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Shoot induction and plant regeneration from cotyledon segments of the muskmelon variety "hógolyó"
61-64.Views:322Cotyledonary segments of the casaba type muskmelon variety "Hógolyó" were used to induce organogenesis. Fifty different hormone combinations were applied to enhance the induction of shoot formation on the edge of the segments. The phases of organogenesis were followed with light- and scanning electron microscope. Shoot induction was achieved with high frequency. The shoots were transferred to hormone free media for root induction. The rooted plantlets were planted out to soil.
NAA was feasible and the method can be applied in transformation experiments.
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Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:288A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
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The effect of soil mixtures of different consistence and phosphorus content in tray transplant-growing by lettuce
71-76.Views:399The transplant-growing with root balls gets more and more popular and time to time the only method even in high-quality lettuce transplant-growing. To work out the technology of transplant-growing in trays it was needed to define the accurate physical and chemical consistency of applied soils.
The transplant production in trays could become a good method in field-grown lettuce technologies. The production of transplants of good quality with this technology could be realised only by accurate soil mixtures.
The matter of transplant-growing substrates could be a low-moor turf. Its qualities could be positively influenced by adding minerals like bentonite in amount of 5 (or 10) V%.
To ensure enough phosphorus for the demands of transplants we have to add more fertilisers. The best results by lettuce we got by adding 4 kg/m3 superphosphate to the soil mixture.
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The effect of timing and IBA treatments on the rooting of plum rootstock hardwood cuttings
7-10.Views:363In propagation of plum by hardwood cuttings, the success of rooting is affected by several factors. Many authors deal with the timing of cutting collections, others investigate the optimal extent of hormonal stimulation. However, there is no data, as yet, about the coherence between these two factors. The aim of this experiment was to find the most advantageous condition s, with regards to both timing and IBA doses, for rentable propagation of the examined varieties. Five varieties were studied: 'INRA Marianna GF 8- 1', 'Myrobalan B', 'MY-KL-A', 'INRA Saint Julien GF 655/2' and 'Feher besztercei'. Cuttings were collected on seven occasions, during late autumn and winter, and were treated with IBA solutions of different concentrations. The optimal dose of IBA was found to be dependent both on the variety and the actual date of cutting collection . Results are reported, along with suggestions for optimal doses and collection periods.
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In vitro regeneration from cotyledons of watermelon
96-98.Views:285Cotyledonary segments of five different genotypes of watermelon were used to induce organogenesis. Five different hormone combinations were applied to enhance the induction of shoot formation on the surface of the segments. The phases of organogenesis were followed with light and scanning electron microscope. Shoots were obtained after four weeks, then the shoots were transferred to hormone free medium for root induction.
This method of regeneration can be applied in transformation experiments. GUS histochemical assay was made to check the expected success of using Agrobacterium for the transformation.
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Effects of biochar and inorganic fertiliser on the growth and yield of beetroot (Beta vulgaris L.) in Kenya
37-45.Views:506Beetroot (Beta vulgaris L.) is a root vegetable packed with many nutritional benefits such as minerals and vitamins. Despite its importance in Kenya, farmers get about 30-35 t/ha which is significantly lower than the potential yield (68 t/ha). This is mostly attributed to low soil fertility. This study aimed to determine the response of the beetroot growth and yield on biochar and NPK. A 3×4 factorial experiment was carried out at Egerton University farm over two seasons to test the effects of biochar and NPK (17-17-17), under supplemental irrigation. Biochar (0, 5, 10 t/ha) was combined with NPK (0, 200, 300, 400 kg/ha). The combination of Biochar and NPK increased significantly (p ≤ 0.05) beetroot growth and yield in two seasons. Treatment B10N400 showed the tallest plants (79.2 cm) at 90 days in season two, while the control resulted in the shortest (27.6 cm). Treatment B10N200 showed the biggest (213.2 cm2) leaves at 90 days. The treatment B5N300 recorded the highest marketable yield (84 t/ha) in season two and the lowest was B0N0 with 2.6 t/ha. Sole application of NPK rates (200, 300, 400 kg/ha) increased significantly the growth and yield of beetroot compared to the control in both seasons. In season one, N300 (300 kg/ha) had 61.9 t/ha of the total yield, the control had the lowest. In season two, 300 kg/ha had 83 t/ha of total yield. Biochar increased beetroot growth and yield in season 2. Treatment B5 recorded the highest marketable yield of 61.2 t/ha, while the control showed the lowest of 53 t/ha.
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Optimizing disinfection protocols for yam explant regeneration in plant tissue culture
66-72.Views:647Yam (Dioscorea species), does not produce commercially viable seeds, and asexual propagation is faced with challenges resulting from carried-over infections from previous generations. Contamination is a prevalent problem in Plant Tissue Culture (PTC), making the development of cost-effective and efficient disinfection protocols crucial for successful PTC. This study aimed to evaluate the effectiveness of yam explant disinfection protocols using various immersion timings, disinfectant concentrations (including ethanol (C2H5OH), sodium hypochlorite (NaOCl), and hydrogen peroxide (H2O2)), and in single or combined disinfectants application. Twenty treatment combinations and one control were assessed on yam vines for Disinfection Efficiency (DE%), Negative Disinfection Effect (NDE%), and the regeneration of shoots and roots (SN & RN) from the culture after 21 days. The study showed that varying immersion times did not significantly impact the evaluated parameters. However, different concentrations of disinfectants resulted in diverse NDE responses. Surprisingly, higher concentrations of NaOCl led to reduced NDE, whereas lower concentrations increased NDE. On the contrary, higher concentrations of H2O2 increased NDE, while lower concentrations decreased it. Shoot and root regeneration rates were also significantly impacted by the choice of disinfection protocol. The research concluded that dual disinfection protocol, specifically 70% ethanol for 7 minutes followed by NaOCl, was most effective for eliminating surface-borne contaminants and achieving successful in vitro propagation of yam plantlets. This method offers a cost-effective solution for establishing microbe-free tissue culture yam plantlets and provides a basis for future research on other Dioscorea plants.
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Grape rootstock - scion interactions on shoot growth and cane maturing
30-34.Views:369Interaction between the rootstock and scion varieties (clones) was examined. Berlandieri x Riparia T. K. 5BB, Berlandieri x Riparia T.5C being the most widely used varieties in Hungary, Fercal, Ruggeri 140 being recently used on special soil conditions ; Georgikon 28 a new established variety and Berlandieri x Riparia T. 8B GK 10 clone were the rootstocks used. The V. vinifera L. varieties were a clone of Italian Riesling and two new hybrids from the same cross population f ( Noble Italian Riesling X Ezerjó) X ( Noble Italian Riesling X Pinot Gris ) }. This study presents the results how the growth habit depends on different scion/stock combinations in the first three years.
Shoot growth characters were observed during the initial years after plantation in the vineyard until we got the first yields. Our results did not agree with those of Pospisilova (1977) that the anticipated vigour of shoot growth will appear later in the vineyard only. However we agree with Zimmerman (1970), who showed that the differences are caused by the rootstocks in the growth habit of vines during the first two years already. The differences in shoot elongation decreased similarly to the rootstocks in the first year of fruit production. It shows us that the intense root development caused vigorous shoot growth, which we think to be a rootstock effect. When the vegetative and generative development are balanced already, the effect of scions in shoot elongation became stronger than the effect of rootstock.
We also found great differences in the maturation of wood each year from planting of the vineyard to the year of fruit production.
During the first years the differences of cane maturation were greater according to the scion varieties, the means were significantly different. In the first ripening year the differences dwindled or increased due to the rootstocks. This means that the effect of the rootstock on cane maturation became stronger in the first year of fruit production.
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Evaluation of morphological parameters and bioactive compounds in different varieties of beetroot (Beta vulgaris L. ssp. esculenta GURKE var. rubra L.)
31-35.Views:792Beetroot consumption based on pickled beets generally in Hungary which is due to the higher yield from second crop harvested in autumn and processed by manufacturing industry. Researches of the past years confirmed its favourable nutritional-physiological effects on human body so demands, as for fresh salad, also increased. The trial aimed at testing the interaction of varieties on quality parameters and in the same time suggestions are made how to use different varieties of beetroot according to its quality. Morphological and sensory evaluations were examined on 10 varieties of beetroot harvested in autumn. The regular spherical shape can reduce the refining loss during the processing of beetroot which is beneficial for the manufacturing industry. In our trial the root shape of Libero, Mona Lisa and Rubin varieties approached most the regular spherical shape (diameter/length – 1.0) which is favoured by not only processing industry but also fresh market. The highest red pigment content (betanin) was observed in Mona Lisa, Akela and Cylindra (34.58–47.66 mg/100 g). A similar trend could be observed in yellow pigments (vulgaxanthins) which proves the close correlation between the quantities of the two pigments (r=0.898). Highest total polyphenol (77.13–83.37 mg GAE/100g) and flavonoid (21.73–22.73 mg CE/100g) contents were detected in Akela, Mona Lisa and Bonel. These varieties are favourable for fresh salad and they can satisfy processing requirements also. Highest water soluble solids content was found in Akela (7.15%). In our conditions nitrate (NO3-N) values below 900 mg/kg were examined in all of the varieties which is favourable in the case of beetroot.
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Effect of different non-chemical treatment methods on organic seed
63-65.Views:215The importance of ecological farming in Hungary is increasing. The utilisation of organic seed is regulated by national law. In our experiment the effect of two plant conditioning substantial: Biokál and Biomit Plussz were tested on the germination of seed. Our test plant species was garden pea (Pisum sativum L.), the most important vegetable in Hungary. The experiment was carried out in the germination laboratories of the National Institute for Agricultural Quality Control after international rules. The germination, the length of root and shoot were measured. 4 hours soaking of pea seeds in 30% solution of Biokál gave the best result. Our results could help to make the field emergence of organic seed faster and safer.
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Irrigation of pear (A review)
65-73.Views:406The plantation of intensive growing orchards and steady increase in yield is essential to return the growing cost by sale. Seasonal crop fluctuation of pear is increased by the frequently occurrence of drought and climatic changes. This study reviews genetic and growing factors determined the alternancy of pear and present the new knowledge concerning on water saving irrigation techniques. Use of dwarfing rootstocks, root pruning, branches pruning and new water saving irrigation make the changes in vegetative and generative growth that successfully improve the alternancy of pear growing. According to publications BA 29 of clonal quince rootstocks exhibited the best protection mechanism against to drought. Regulated deficit irrigation (RDI) applied during rapid shoot growth and slowly fruit growth result a decrease in shoot growth and 60% of water saving in pear orchard while there was no influence on harvested yield. Partial rootzone drying (PRD) microjet irrigation applied in pear orchard result 23-52% of decrease in water use, however concerning explorations are contradictory. Further investigations need to improve the efficiency of new irrigation technology adapted pear varieties based on monitoring of soil water status and measurement of stem water potential as stress indicators of plants.
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Possibilities and limits of use plastic constructions in fruit growing technologies
71-75.Views:855On the Experimental Station Pallag of Debrecen University different combinations of fruit species and rootstocks have been raised under and without plastic foil cover in 2002. The growth and productivity of the grafts was our objective of comparison. Results revealed substantial differences in fruit set and yield depending on species and varieties. Sweet and sour cherry varieties grew much shorter under the plastic cover, whereas apricots, peaches and plums set fruit much more eagerly compared with the trees outside the plastic cover. Outside the plastic cover, the trees were much more developed at the beginning of their fruiting period. In spite of that, the growing processes were more intense under the foil. The differences are allegedly due to the repeated summer pruning necessary under the restricted space of the foil, on the other hand, due to the root concurrence because of the dense planting. Regarding the inner properties of the fruits, soluble solids, sugar and acids were higher outside, whereas macro- and mezzo-elements (P, K, Ca, Mg) were more abundant in fruits grown under the foil cover. Further efforts to explore those relations with other varieties and rootstocks are justified.
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:396The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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Factors affecting rhizogenesis in vitro and acclimatisation of three cherry rootstocks
40-46.Views:237Factors affecting rhizogenesis in vitro and acclimatisation of three rootstocks of cherry, i.e. Mahaleb, Maxma-14 and Weiroot -10 were investigated.
Rooting was easily achieved within 2-4 weeks on MS-based liquid or agar-gelled media containing auxins IBA at conc. 0.49 or 2.45 pM or NAA at conc. of 0.49 pM. On liquid media with 2.45 pM IBA, a maximum rooting efficiency of 95-100% was obtained. However, high concentrations of auxin delayed the time of root initiation for 3-5 days.
Rooted plantlets were transplanted into pots with a mixture of 3:1 (v/v) peat:perlite and acclimatised gradually to field conditions with efficiency of 60%.
