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Evaluation of morphological parameters and bioactive compounds in different varieties of beetroot (Beta vulgaris L. ssp. esculenta GURKE var. rubra L.)
31-35.Views:413Beetroot consumption based on pickled beets generally in Hungary which is due to the higher yield from second crop harvested in autumn and processed by manufacturing industry. Researches of the past years confirmed its favourable nutritional-physiological effects on human body so demands, as for fresh salad, also increased. The trial aimed at testing the interaction of varieties on quality parameters and in the same time suggestions are made how to use different varieties of beetroot according to its quality. Morphological and sensory evaluations were examined on 10 varieties of beetroot harvested in autumn. The regular spherical shape can reduce the refining loss during the processing of beetroot which is beneficial for the manufacturing industry. In our trial the root shape of Libero, Mona Lisa and Rubin varieties approached most the regular spherical shape (diameter/length – 1.0) which is favoured by not only processing industry but also fresh market. The highest red pigment content (betanin) was observed in Mona Lisa, Akela and Cylindra (34.58–47.66 mg/100 g). A similar trend could be observed in yellow pigments (vulgaxanthins) which proves the close correlation between the quantities of the two pigments (r=0.898). Highest total polyphenol (77.13–83.37 mg GAE/100g) and flavonoid (21.73–22.73 mg CE/100g) contents were detected in Akela, Mona Lisa and Bonel. These varieties are favourable for fresh salad and they can satisfy processing requirements also. Highest water soluble solids content was found in Akela (7.15%). In our conditions nitrate (NO3-N) values below 900 mg/kg were examined in all of the varieties which is favourable in the case of beetroot.
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Propagation of plum rootstocks by hardwood cuttings
23-28.Views:131Vegetative propagation by hardwood cuttings is a very simple and cheap method for production of plum rootstocks. The aim of this study was to examine if this propagation technique is suitable for practice of three plum rootstocks and find the time or period when the percent of rooted cuttings is maximal. Based on our results, hardwood cuttings of the rootstocks tested have the rooting potential acceptable for practice, however, for Fehér besztercei in the previous literature leafy cuttings are recommended. Fehér besztercei reached 74.0% rooting, cuttings of Sainte Julien GF 655/2 rooted in as high percentage as 78.3%, and Marianna GF 8-1 had 88.3% rooted cuttings. Rooting potential of hardwood cuttings depends on more factors, one of them can be their dissimilar sensitivity for the diverse environmental circumstances at the different propagating dates, affecting through the internal biochemical changes that can be in relation with the differences in their dormancy.
The cuttings of Marianna GF 8-1 take root easily, but in some years the conditions were less favourable for reaching maximal rooting. For taking cuttings the period from the beginning of October until December was optimal. For Fehér besztercei the optimal date of cutting collection was around the end of October, but in some years the rooting in the middle of December was also high. Sainte Julien GF 655/2 definitely rooted best in October. The treatments with different IBA concentrations in two years affected differently the rooting percentage. The rooting of Marianna GF 8-1 and Sainte Julien GF 655/2 is barely influenced by the different hormone dose in both years. Hardwood cuttings of Fehér besztercei rooted definitely better when treated with 2000 ppm IBA in comparison to untreated ones, while in 2001-2002 there was no difference between 2000 and 4000 ppm.
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Possibilities and limits of use plastic constructions in fruit growing technologies
71-75.Views:245On the Experimental Station Pallag of Debrecen University different combinations of fruit species and rootstocks have been raised under and without plastic foil cover in 2002. The growth and productivity of the grafts was our objective of comparison. Results revealed substantial differences in fruit set and yield depending on species and varieties. Sweet and sour cherry varieties grew much shorter under the plastic cover, whereas apricots, peaches and plums set fruit much more eagerly compared with the trees outside the plastic cover. Outside the plastic cover, the trees were much more developed at the beginning of their fruiting period. In spite of that, the growing processes were more intense under the foil. The differences are allegedly due to the repeated summer pruning necessary under the restricted space of the foil, on the other hand, due to the root concurrence because of the dense planting. Regarding the inner properties of the fruits, soluble solids, sugar and acids were higher outside, whereas macro- and mezzo-elements (P, K, Ca, Mg) were more abundant in fruits grown under the foil cover. Further efforts to explore those relations with other varieties and rootstocks are justified.
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Histological studies on some native perennials
79-82.Views:132Growing of native perennial species became more and more popular in the last ten years. In order to obtain more information on their histological structure, investigations were done on Aster linosyris, Inula ensifolia and Prunella grandiflora. The histological features are usually relating to the plants' ecological demands which is an important aspect in their growing. Differences were found in the structure of the stem of Asteraceae and Lamiaceae members. While separated vessels were formed in the stem of Aster linosyris and Inula ensifolia, continuous vessel-system forms in the stem of Prunella. Alternating segments of collenchyma and chlorenchyma are found in the stem of Aster linosyris, while palisade parenchyma is situated both on the abaxial and adaxial surface of the leaves. Vessel-system of the root is tetrarch. Histological structure of the stem of Inula ensifolia differs from Aster linosyris in the broader cortical parenchyma which is composed of approx. 8-12 cell layers. It contains neither collenchyma nor chlorenchyma. In the stem of Prunella grandiflora a nearly continuous vessel-ring is formed from the four primary vessels. Long, multi-celled hairs were observed in the district of angles of the stem.
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:211The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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The evaluation of grape vine decline pathogens in the experimental field of the Georgikon Faculty of Agriculture in Cserszegtomaj
19-22.Views:166Vine decline causes important economic loss in viticulture, especially in longer view. Several causal pathogen were described lately, however little is known about the etiology or epidemiology of these pathogens on grapevine rootstock. It is well known that these diseases affect grafted and rooted grapevines and are not specific to any scion-rootstock combinations. Our aim was to determine what pathogens are presents in the experimental field, especially causal agents of the rootstock decline. Different grapevine rootstocks and scion varieties were tested in our trial. Isolations were made from the wood tissue and pathogenity tests were done with isolated Cylindrocarpon destructans. The possibility of infection during the propagation process was studied as well. Most commonly Cylindrocarpon sp. and Phomopsis sp. species were identified from the examined varieties. Cylindrocarpon destructans was able to spread to apical (shoot) and basal (root) direction from the point of infection with uneven speed. Callus development is not inhibited by the fungi causing the leaf symptom of the vine decline. Shoot development is reduced if unhealthy parts are grafted to each other.
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Studies on the essential oil of different fennel (Foeniculum vulgare Mill.) populations during onthogeny
27-30.Views:203In the recent studies two Foeniculum vulgare Mill. populations (Hungarian and Korean) had been studied in open field trials in 1997-98 in Budapest for the identification of their essential oil characteristics. The essential oil accumulation level as well as the composition of the oil were studied during ontogenesis and at 6 phases of the umbel development from budding to overripening.
In the vegetative phases, the accumulation level of the essential oil was higher in the Hungarian genotype. In the leaf rosette stage, anethole is the main compound in both genotypes (40-96%), except the root of the Korean populations which contains 54% dillapiol. The accumulation level of anethole is slightly decreasing, while dillapiol is slightly increasing during the ontogeny. a- and [3-pinenes are characteristic compounds of the leaves, especially in the Korean genotype (10-11%). Before seed setting, fenchone was present in a considerable amount (7.5%) only in the umbels of the Hungarian genotype.
During the generative development, the maximum values of essential oil content are reached at the milky fruit stage (10,11%) in the Hungarian. and at the green fruit stage (7.1% ) in the Korean type, while the composition of the essential oil changed only slightly. The ratio of fenchone is increasing after flowering and being stable during ripening. Anethole varies to a smaller extent in the umbels, only.
We proved, that the dinamics of essential oil accumulation and the oil composition may vary according to genotype. Based on our results, the Korean population is characterized in the system of Bernáth et al. (2) as a high anethol-low methyl chavicol chemoform of the anethol chemovariety (fenchone<15%; anethole>68%; methyl chavicol< 3,2%).
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Evaluation study for the effect of four mango rootstocks on behaviour of Keit mango variety
49-53.Views:260Our investigation was carried out in four mango rootstocks were namely Zebda, Socaria, 13/1 an 4/9. This study reviewed the effect of these rootstocks on growth behaviour of Keit mango variety as a scion grown on them. This evaluation and comparison included data on following aspects: the vegetative growth characters, root growth parameter, uptake of some macro nutrient and some organic substance. All results in this regard refer that the Socaria rootstock gave the highest values off all parameters so it is considered as a vigorous rootstocks, means that the trees on this rootstocks will reach to high size after short time. By contrast, Zebda rootstocks gave the lowest values in this concern and this will reflect on tree size on it. This means that the Zebda rootstocks will be useful in the system of high density orchard comparing with Socaria. Concerning to other rootstocks, they were intermediate between Socaria and Zebda rootstocks.
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Dr. Ottó Orsós, the forgotten Hungarian pioneer in plant tissue culture
9-13.Views:176The knowledge of tissue culture deserves attention in respect of understanding the development of universal biology. This study intends to contribute to the past of the plant tissue culture by such data of the history of science which have been unprocessed so far. It seems that the life-work of the Hungarian biologist, Dr. Ottó Orsós is a missing and essential link between those early plant hormone researchers and the representatives of the pioneers of tissue culture schools who have contributed substantially to the development of the modern in vitro plant morphogenesis and plant cell biology. Orsós cultured kohlrabi tuber cubes on White culture medium in a sterile manner. This way, he could efficiently direct the in vitro morphogenesis of the kohlrabi, the regeneration of its shoot and root, and the formation and steps to subculture of pure callus tissues in 1938. He supported the correctness of its statements by means of detailed anatomical examinations. Orsós successfully rooted and aclimatized complete regenerated plants. We may as well call the above system — in remembrance of the creators of the original concept — "Haberlandt-Orsós model". Between the publishing of his main paper in 1938 and 2003, a period of 65 years has lapsed. On the occasion of this anniversary, we bow before this forgotten pioneer.