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Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods
35-41.Views:286Symptoms resembling phytoplasma disease have been observed on peach trees in a seed-source plantation of stone fruits in south Hungary quite recently. In this publication we report on the results of woody indexing of symptomatic peach trees on GF 305 indicator in the field and under greenhouse conditions as well as on molecular studies. Phytoplasma infection detected on GF 305 indicators in greenhouse and field indexing was confirmed by PCR. Nested PCR was conducted using universal primer pairs followed by group and subgroup specific primers for the second amplification. RFLP analysis of nested PCR products was performed using Rsal restriction enzyme. Based on the results of molecular studies it can be concluded that phytoplasmas, belonging to the European stone fruit yellows subgroup (16SrX-B) were identified in peach trees. Further studies on symptomatic peach trees originating from different parts of Hungary are in progress.
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Effect of different cultivation methods on strawberry’s antioxidant value
7-10.Views:678The small fruit and strawberry production is economically important in Serbian agriculture. The fresh fruit is sold in domestic markets, but the frozen and processed is mainly sold in foreign markets. The growers find themselves in an ever-expanding selection of the varieties, but besides the nursery and breeder’s descriptions of new cultivars, there are only few exact results for the biological and economical ability of the varieties, which makes easier to choose the proper cultivar. The performance of the varieties in modern strawberry production is worth to investigate by adaptive cultivation methods and production technique. In the case of new strawberry varieties we know neither their needs in production nor their real capability, so they have to be compared with an older, standard control variety (‘Clery’). The breeder’s manuals give only references for the needs of the production technique, of the variety, so it is necessary to have facts, which are based on objective testing results. In this work, a new variety (‘Joly’) is being compared with the control variety. Comparative study of individual varieties are required covering the antioxidant content of the fruits (FRAP) and variability of these amounts in different years of breeding as well as the production techniques under similar circumstances.
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Morphological and phenological properties of sour cherry varieties grown in Hungary and their inter-incompatibility relations
114-117.Views:1230Regular observations and experiments were performed during a 14 year period on 6 sour cherry varieties. The morphological traits of leaves and fruits were compared, and the phenology of blooming as well as of ripening dates served to start an estimation of the possibilities of mutual pollination and the planning of harvest operations. Experiments involved obligate autogamy, artificially controlled allogamy and open pollination in order to reveal self-fertility, self-sterility or inter-incompatibility relations.
The varietal characters represent, each, different values in the distinction of the items, because of their intra-varietal variability. From that point of view, the most reliable are the data of blooming and ripening time, fruit size and the fertility relations.
Inter-incompatibility was observed between the group of self-fertile, "Pándy type" varieties (`Újfehértói fürtös’, ‘Debreceni bőtermő’, ‘Kántorjánosi’) on one side and the selection of Pándy 7', a self-sterile variety on the other side. Unilateral incompatibility has been detected within the former group of new, self-fertile varieties, the combinations: (`Újfehértói fürtös’ x ‘Debreceni bőtermő’ as well as `Újfehértói fürtös’ x Kántorjánosi’.
Our results prove the close kinship between those three new varieties and the original Pándy variety on the base of being highly similar in their morphology and also of the fact of their inter-incompatibility, though unilateral.
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New sweet cherry cultivars in intensive plantings
13-16.Views:1129The study took place in the largest sweet cherry plantation in West Hungary. The purpose has been the identification of those varieties, which will be suitable for intense cultivation, early fruiting and excellent fruit quality, moreover, the selection of the optimal phytotechnical procedures. At the same time, scion-rootstock combinations have been tested also from the point of view of growing intensity and fruiting in high-density plantation. The dense planting is induced to start fruiting early and yield regularly by special methods.Yielding was stimulated by maintaining the balance of vegetative-generative growth by binding the shoots, by summer pruning, by cuts on the trunk and root pruning. Best experiences have been found in yield and quality in the following varieties: Canada Giant, Carmen, Firm Red, Giant Red, Katalin, Kordia, Regina. Dense planting has been feasible also on vigorous rootstock, like P. mahaleb. Dwarfing rootstocks like P-HL-A, Gisela 6, accelerate the formation of flower buds and yielding earlier with fruits of adequate size. ‘Firm Red’ and ‘Giant Red’ excelled with their large fruit (>27 mm diameter) in all combinations, thus being promising under Hungarian conditions.
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Spectral properties of strawberry plants
17-22.Views:567Strawberry varieties were studied in 3 different growing areas of different soil- and ecological properties in Hungary. We have measured the optical spectra of the leaves together with their nutrient content and performed sensory examinations on the deep frozen and melted fruit. The 6 varieties were Elsanta, Marianna, Spadeka, Symphony, Camarosa, and Raurica. The 3 sites were Újfehértó, Pölöske, and Kecskemet. Colour of the leaves was characterised by their lightness and dominant wavelength. Under non-optimal soil and ecological conditions the varieties could be distinguished by their colour — the leaves became lighter and more yellow. The fruits did not produce the genetically determined taste if the plant can not grow in optimal circumstances — we found a negative correlation between the leaf colour in flowering time and the fruit flavour. Leaf colour measurements performed in flowering time make it possible to improve fruit quality by changing the nutrient supply between flowering and ripening.
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Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
79-80.Views:336About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible.
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Exotic woody plants inclining to escape in the Buda Arboretum under strong urban effect in Budapest
93-97.Views:301The Buda Arboretum of the Szent István University is situated in the western-middle part of the city of Budapest. It contains 1640 taxa of woody plants on an area of 7.5 ha. The sheltered position and the urban micro- and meso-climate is favourable for warm-loving, even subtropical plants, of which many species not simply stay alive, but flower, bear fruits and are spreading by seedlings in suitable spots. These instances call our attention to the possibilities that urban climate offers for landscapers, but also to over 110 species, which can be garden escapes in the future. Full list of plants, which tend to escape in the Arboretum is given in the paper.
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Irrigation modeling in a pear orchard
75-79.Views:929The pear has large water requirement, therefore the planting of high density and grass covered pear orchards are needed irrigation
conditions in Hungary. Drip irrigation spread in the orchards is due to the 90–95% of water use efficiency. One of the key role of irrigation is the
proper determination of evapotranspiration and crop coefficients. As there is a considerable lack of information for different crops or fruits the
Penman-Monteith method is used for the estimation of evapotranspiration, using CROPWAT 8.0. The research field was the genetic collection of
pear at Újfehértó, in Hungary, which is situated in Nyírség meso-region. Our aim was to establish drip irrigation at this site. Based on the results
of CROPWAT irrigation model the mean amount of the total gross irrigation is between 230–270 mm, within 3 irrigation interval regarding
climatic and rainfall data of the last 10 years. In 2009, due to heavy drought, the total gross irrigation was 355,4mm/year on sandy soil calculating
with 45% total available water depletion in 5 irrigation interval. The sizing of the irrigation system was set to the maximum 0.55 l/s/ha, which is
6.3 l/tree/h. 6.3 l/tree/h can be carried out with a drip emitter having 16 mm wing lines diameter, 4 l/h water flow at 3 atm pressure. -
Alternate bearing of 'Golden Reinders' and 'Summered' apples
77-85.Views:1115The aim of the present study was to study the effect of biennial bearing (irregular yields) on the generative production of apple cultivars 'Golden Reinders' and 'Summerred' on M.9 rootstock. The observations were made at Nagykutas in West-Hungary for four years. The authors have studied the flowering time, flower density, fruit density, fruit drop, seed content, yielding and fruit quality in the on-year and off-year. Based on the results, it can be stated that the alternation does not cause a significant change in the flowering time of cvs. 'Golden Reinders' and `Summerred': the difference between the flowering time of trees in the on-year and off-year period was 0-1 day. However, there were significant differences in the flower density. 'Golden Reinders' proved to have a weaker tendency to alternation similarly to the statements of the literature. Alternation (off-year) was observed only in three years from the studied four years. While in the case of `Summerred', strong alternation was observed in all four years. In fruit density, there were differences among the cultivars and among the different cycles of biennial bearing. Fruit density (and its opposite, fruit drop) changed inconsistently in the period between flowering and harvest. In the case of 'Golden Reinders' and 'Summerred' 2 and 4 fruit drop periods were detected, respectively. In the off-year, the different periods were less distinct, in most cases they were overlapping each other. The dynamics of fruit drop was related to seed content per fruit. The lower the seed number was, the higher the degree of fruit drop was. In both the high- and off-year cycles, the number of seeds in fruits on the tree and on the ground increased with time. In the yield parameters (fruit number, fruit load, yield efficiency), the differences were greater among the yields of trees in the high- and off-year cycles for 'Summerred'. The fruit quality parameters were greatly influenced by the fruit load of the trees in the different alternation cycles. In general, it can be stated that fruit mass, diameter and height were lower on trees in the on-year. A similar trend could be observed in flesh firmness, cover colour and dry matter content, but the differences were smaller. 'Summerred' was more sensitive to the differences in fruit load.
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Experimental results of the effects of Hungarian climatic conditions to German disease-resistant industrial apple varieties
53-56.Views:217In the recent years, several disease-resistant apple varieties appeared through the modern breeding technologies. These varieties can be grown with low usage of pesticides, which mean not only environment friendly fruit growing, but the production costs are also lower. In Eastern-Hungary — it is one of the main apple growing regions — a new apple growing structure started to form by the investment of the German Wink Ltd. — several resistant apple varieties were brought from Germany. 'Resistant' refers genetic resistance that usually transferred from the genome of wild apple species. But the fruit of these apple species is not only resistant to diseases, but its quality is poorer, too. In Germany the Re-apples are grown only for the processing industry. Due to climatic circumstances in Eastern Hungary, the first experiences showed better parameters during laboratory measurement, the fruits have more beautiful view, shape and inner characters than usual industrial apples.
In our paper we discuss the results of sensory (consumer) tests, carried out in Eastern Hungary and in the Budapest-region the data analysis of systematic storing experiments (refraction, flesh firmness, weight loss, etc.) and profile analysis of fresh and stored Re-apples. (In the profile analysis the ProfiSens software [4,5] has been used.)
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Pollen morphology of fruit species
49-57.Views:13875Size and surface morphology of pollen has been studied in 87 twit varieties of 10 fruit species during the period of 1990-1995. No preceding work of that type came to our knowledge, yet.
The samples comprised a wide variety of cultivars included male sterile, self-incompatible, partially self-fertile stone fruits, diploid and hexaploid plums, diploid and triploid apples.
The large number of species and varieties facilitated the comparison of items within and between the respective species.
It was stated that the size, shape and surface morphology of pollen is genetically determined and those data, combined with other variety characters, are suitable for the classification and distinction of varieties.
In assessment of pollen size and shape, their moisture content is crucial. The major diameter of the swollen pollen as well as the length and width of the dry grains are characteristic to species and/or to variety.
The width and shape changes largely with moisture content. Large grains are proper to quince, apricot, peach and almond, medium sizes are found in apple, sweet cherry, sour cherry, European plum, whereas small size is typical to Japanese plums.
The low number of varieties studied does not allow conclusions concerning differences within pears, quinces and almonds as species. In the rest of species, valid differences have been registered as between varieties.
Within species, as apple and plum, the effect of ploidy (i.e. number of chromosomes) was expressed in the size of their pollen. In stone fruit species, the correlation between size. of anthers and size of pollen grains was positive.
Genetic relations between the self-fertile sour cherry varieties of the Pándy type (Debreceni bőtermő, Kántorjánosi, Újfehértói fürtös) as well as the self-incompatible apricots of "giant" fruit size are supposed to be analysed by pollen studies but there did not turn out any decisive conclusion, yet. Other characters also should be considered.
The assembly of pollen characters is decisive in the determination of the variety. The ratio of empty pollen grains, the grain size and the density as well as the size of the pits on the surface are best suited to distinguish pollen lots.