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Qualitative detection of genetically modified organisms in plant samples
309-313Views:602We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Nutrition reaction of different varieties of winter wheat (Triticum aestivum L.) bred in Karcag
63-67Views:360In our study the nutrition reaction of four varieties of winter wheat (KG Bendegúz, KG Kunhalom, KG Kunkapitány, KG Széphalom) has been investigated. In the experiment the effect of twenty different nutrition doses on the yield and thousand kernel weight of the wheat varieties has been studied. Significant difference could be figured out among the yield and thousand kernel weight of the wheat varieties, so the choice of the proper variety determines the quantity of yield. KG Kunkapitány had the highest yield, while KG Széphalom had the highest thousand kernel weight in the experiments.
Investigating the effect of the nutrients on the yield the conclusion was that all nitrogen doses had significant yield increasing effect compared to the untreated plots, but among the 40, 80 and 120 kg ha-1 doses there was no statistical difference. In the case of phosphorus the 40 kg ha-1 dose showed statistically verifiable increase of the yield, while potassium doses have no influence on the yields. The thousand kernel weights reached the maximum values where the highest nutrient doses were applied.
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Examination of nutrient reaction of winter wheat after sunflower forecrop
9-13Views:263We tested the fertilizer reaction of four different winter wheat varieties in three different crop years, on chernozem soil, in long-term experiment. We examined the optimum fertilizer requirements and the maximum yield of the varieties. According to our results there were significant differences among the years: the yield of the winter wheat varieties changed between 1.4–6.1 t ha-1 in 2013, 3.8–8.6 t ha-1 in 2014 and 3.2–8.6 t ha-1 in 2015. The yield increasing effect of fertilization was significantly different in the tested years. The optimum level of fertilization was determined by, besides the genetic differences among the varieties, the crop year and the extent of fertilization. In milder winter months, due to the higher average temperatures, yields of winter wheat increased compared to an average crop year.
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Examination of the Nutrient and Water Utilization of Different Corn Genotypes in the Hajdúság
55-59Views:224The research was set up on chernozem soil at the Látókép MÉK research area of the University of Debrecen in Hungary. We examined the following factors of the hybrid P9494, P9578, PR37N01 and PR37M81 in 2013.Yield, yield production per 1 kg fertilizer, water utilization and nutrient reaction. We found that the best yield results were achieved at level N120+PK in case of hybrid P9494 (17 132 kg ha-1) P9578 (16 838 kg ha-1) and PR37N01 (17 476 kg ha-1) and at level N150+PK for hybrid PR37M81 (16 754 kg ha-1). Results of yield per 1 kg NPK studies indicate that the most intense yield growth occurred at level N30+PK compared to the control treatment. This means that yield production per 1 kg NPK was 39.2 kg kg-1 in the case of hybrid P9494, 54.2 kg kg-1 in the case of P9578, 17.6 kg kg-1 for PR37N01 and 44.2 kg kg-1 in the case of PR37M81. After comparing corn hybrids’ water utilization, our conclusion was that the control treatment achieved the worst results in the case of each hybrid (P9494: 20.8 kg mm-1, P9578: 21.0 kg mm-1, PR37N01: 26.2 kg mm-1, PR37M81: 19.5 kg mm-1). For hybrid P9494, P9578 and P37N01, the best water utilization results were measured at level N120+PK (31.5 kg mm-1, 31,0 kg mm-1 and 32.1 kg mm-1), while PR37M81 reached the highest values at level N150+PK (30.8 kg mm-1). We determined the hybrids’ nutrient reaction and its optimum fertilizer values. The best nutrient reaction results were achieved by hybrid PR37N01, while that of hybrid PR37M81 proved to be the weakest.
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Reference genes for livestock gene expression profiling – Literature review
81-89Views:523Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments. -
Examination of Hybrid-specific nutrient supply at corn on chernozem soli
91-95Views:461The effect of increasing fertilizer dosages on the yield of eight different maize hybrids (SY Ondina, NK Kansas, NK Lucius, NK Octet, NK Thermo, SY Flovita, SY Brillio, NX 47279) has been investigated in the crop-year of 2011. According to our results it can be stated that contrarily to the control treatment the application of different nutrient-levels has resulted a significant yield increment (2 000–5 800 kg ha-1).
Based upon the results of this experiment we have drawn the conclusion that the nutrient level of 120 kg N+PK was the optimal for the investigated hybrids. The highest yield (14 475 kg ha-1–15 963 kg ha-1) of the hybrids with different genotypes has been produced in case of this fertilizer treatment. With the comparison of the control and the optimum-fertilizer treatments the yield-increasing effect of mineral fertilization and the different reaction of hybrids towards increasing fertilizer dosages have been proven. In case of the control treatments the best-yielding hybrids were NK Thermo (11 917 kg ha-1) and NX 47279 (11 617 kg ha-1). Contrarily on the optimal nutrient supply level the hybrids SY Brillio (15 876 kg ha-1) and NX 47279 (15 963 kg ha-1) have produced the highest yields. Summarizing, we can state that the hybrid NX 47279 has resulted stable and high yields in the fertilized treatments. Analysing the yield-increasing effect of 1 kg fertilizer active substance it was proven, that the hybrids SY Flovita (45.43 kg ha-1), SY Brillio (44.47 kg ha-1) and NX 47279 (42.33 kg ha-1) had a good reaction towards even lower nutrient supply levels as well. In case of the control treatment the average water utilization coefficient of the hybrids was significantly lower (35.2 kg mm-1), than in case of the optimal nutrient supply level (N120+PK) treatments (48.9 kg mm-1).
Therefore the hybrid specific difference between the water utilization of genotypes could be revealed. -
Productivity and Nutrient Reaction of Maize Hybrids
78-83Views:325Several factors influence the quantity and stability of maize yield, the most important being the nutrient supply, the hybrid and precipitation. In 2004, during the maize growing season the precipitation was more than the 30 year’s average, with 68.3 mm, but the distribution was unfavorable. The experiment was carried out in Debrecen at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization.
The favorable results reached were due to the rainy season. The average yield varied between 7.78-9.67 tha-1. The DK 440, PR37M34, PR38A24, PR39D81 and PR36R10 of the hybrids reacted to higher fertilizer doses with significant growth yields, the yield (more than 11 tha-1) was the highest for N200, P125, K150 fertilizing. The other hybrids, DKC 5211, Mv Vilma and MV Maraton, gave similar results at the N120+PK fertilizer dose and the ensuing doses depressed the yield. Fertilization was more effective thanks to the precipitation. The fourth and fifth dose increased or decreased maize yield depending on the nutrient reaction of the hybrid. The agro-ecological optimum of NPK fertilization was N120, P75, K90 kgha-1.
During the experiment we tested the moisture loss of five hybrids. The rainy crop year’s effect on the seed moisture content at harvest was higher than in previous years. The seed moisture content hybrids at harvest which have shorter crop years (FAO 300) was 18-19%, and hybrids with longer vegetation periods had more than 20% seed moisture content. DK 440 hybrid had the intensive moisture loss of the five hybrids, at the start of the measurement, the seed moisture content was higher than 40%, and it decreased to 18.6% by harvesting. -
Identification and biological examination of some inland Monilinia species
27-29Views:341The aim of this study was to identify and biologically analyse some Monilinia species from Hungary. 146 M. fructigena and 28 M. laxa species out 174 infectious fruit from all over the country were used for the study. For further study 10 isolates were used and apple fruit was inoculated with them according to Koch postulate. 1–2 mm ochre exogen stromas were observbed on infectious plant parts and growing signs on culture of all isolates were identical to M. fructigena. To affirm classical identification, isolates with molecular biological method were also prepared using PCR reaction. Control isolates of M. laxa, M. fructigena and M. fructicola were used. The size of PCR product showed that all isolates had a 415 bp band which was typical of M. fructigena. Results support the previous observation that M. fructigena and M. laxa species occure all over Hungary.
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Evaluate the nutritional reaction at winter wheat after different forecrops
77-80Views:226Our field researches took place on the Látókép test farm of Agricultural Science Centre of University of Debrecen, Centre for Agricultural and Applied Economic Sciences, in long-term experiment, on calcareous chernozem soil, in growing season of 2014/2015. In our experiment we examined the fertilizer reaction and the yield of different winter wheat genotypes (GK Öthalom, GK Csillag, Mv Csárdás, Mv Toldi) with grain maize and sweetcorn forecrops. According to our results, the sweetcorn forecrop strongly affected the yield. In the average of the fertilizer treatments and the varieties, after sweetcorn forecrop 6.9 t ha-1, after grain maize forecrop 5.4 t ha-1 average yield was gained. According to our data, the fertilizer reactions of the varieties were significantly different.
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Stress Response of four Common Carp (Cyprinus carpio L.) Varieties with Different Genetic Background
26-29Views:170Four different carp varieties were tested for stress reaction, with the help of 10 °C cooling and 100 g/l crowding. The selected varieties represented a broad genetic spectrum of carp species. The stress was monitored by measuring plasma cortisol, glucose and lysozim levels. In each test different varieties showed significantly different reaction to stress. This means that they differ in the trait of general resistance. Thus, this trait can be used in selection programmes and the acquired information helps to understand fish-environment interaction and specific resistance. These traits should be used in selection to improve the breeding of new more tolerant species for intensive farming or for organic production.
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Evaluation of long term experiments from a new aspect
55-60Views:391During our work, we developed a new, simple method to show the effects of fertilization on yield, which can both be applied over the long term as well as in series of independent experiments.
During the testing of this method, at the experimental farm of the Debrecen University Center for Agricultural Sciences at Látókép on a chernozem soil with lime deposits, we examined the fertilizer reaction of maize hybrids between 1989 and 1994. The treatments were: winter tillage, plant density of 70-80 thousand, unfertilized, N 120, N 240 kg/ha fertilized treatments, long term experiments using Dekalb 524 and Volga SC hybrids in long term experiments.
Four parameters are shown in the model. In the examined period TRmax represents the greatest yield in the fertilized treatments, NT the yield in unfertilized treatment, k the „efficiency of fertilizer” to NT and b the depression-coefficient, where the expected value is zero. The expected grain yield of the fertilized treatments (Y), in the function of the unfertilized grain yield (x) is the following:
The parameters were determined using the Monte Carlo method, in the optimizing process the sum of deviation square was minimized. The correct conformation of the functions was determined by the greatness of the R-value and the standard error. We found that during six years of testing, the tendency of fertilization efficiency was similar in the case of both hybrids. There was an unfavorable weather interval and, in these years, the yields were low, fertilization did not have an effect and moreover, in extremely bad conditions resulted in an obvious yield decrease. With the improvement of conditions, which in the case of our country means an increase in precipitation, the efficiency of fertilization increases and reaches its peak at 13-14 t/ha. At this point, the yield increasing effect of fertilization is 4-4,5 t/ha. If the yield of the unfertilized treatments increases from 8-9 t/ha, then the efficiency of the applied fertilizer decreases.
Most likely, the k and b parameters depend on the soil of the experimental location (nutrient and water management) and on the amount of pplied fertilizer and the characteristics of the hybrid. With the increase of fertilizer dosage the k-parameter also increases. The greater value though does not obviously mean a more favorable situation. It is true that in medium and good years this means great fertilizer efficiency, but in low or extreme precipitation conditions it also means greater risk. With the increase of the k-parameter, the yield deviation also increases which, from a cultivation point of view, is quite unfavorable. If the value of the b-parameter is other than, zero then the effect is clearly unfavorable, because with the increase of this value, the yield decrease is also greater. The fertilizer reaction of the two examined hybrids can be well characterized by these two hybrids.
Examining the six years, our created model estimated the effect of fertilization on the yield accurately and with a high degree of safety. Both in highly unfavorable and extremely good years, it gave an exact estimate. In our opinion, it can be used well to evaluate the effects of fertilization on yield in the future. -
Methods for the examination of the role of the time factor in managerial and organizational processes
24-32Views:187managerial efficiency. The system of investigations is in accordance with the method elaborated and applied by the Department of Management Science of UD, CAS, Faculty of Agricultural Economics and Rural Development (Berde, 2003). This method involves questionnaire interviews and corporate data collection. The first part of the questionnaire contains data with respect to the company, while the second part focuses on the manager being interviewed. The third part is a closed, directed interview-based preference assessment. I aim to assess the sample at my disposal in order to test the research model.
In the course of the assessment of questionnaires, I apply descriptive statistical methods for the assessment of the personal and professional features of the managers interviewed and the characteristics of the companies. In the case of questions substantively concerning the time factor – resource, managerial function, reaction time, and impact assessments – I carry out hypothesis testing. I draw up a zero hypothesis, whose appropriateness I test by means of statistical methods. I usually apply rank correlation for the evaluation of the relationship between the variables measured on an ordinal scale in my investigations. This can be applied if not the exact values of the given variables, but only their order in one respect or another is known. If the group building criterion is two-variable Mann-Whimey, if a multi-variable Kruskal-Wallis trial can be applied, which, after the rank transformation, gives information about the acceptance or refusal of the Ho hypothesis through the average of the ranks. The model also contains the managers’ evaluation of the time factor in management work. -
Evaluation of reaction to plant density of sunflower hybrids by regression and Kang’s stability analysis
101-109Views:318The world production area and the total production of sunflower has significantly been growing. The harvested yield was 23.4 and 21.1 million ha in 2005 and 2001, respectively. The total sunflower seed production has also unexpectedly increased.
Although sunflower is produced on lower quality soils in Hungary, in 2005 the average harvested yield was 2.43 t ha-1, which was the highest in the world.
Sunflower is a typical commercial plant and fits well in the crop structure. Since in terms of acreage the most significant crops are corn and cereals, the partial monoculture cultivation cannot be avoided. Sunflower production is a way to eliminate this problem, therefore it has an important role both in cultivation and ecological points of view.
Accordingly, sunflower has an important role in reducing the monoculture cultivation of some plants, as well as increasing biodiversity. Sunflower well adapts to Hungary’s climatic conditions and its production is easily practicable in our country.
The reaction of sunflower hybrids on crop density change is different. Some hybrids are more some are less sensitive to this parameter. In different crop years, the crop density optimums of the different genotypes are also different. In Hungary, the yield and quality is primarily determined by fungal infections, while viruses and bacteria are less important.
The research was conducted at the Látókép farm and Regional Research Institute of the University of Debrecen, Centre of Agricultural Sciences. The research institute is situated by Road 33,15 km from Debrecen in the Hajdúság. The duration of the experiment was seven years, 10 hybrids were examined in each year.
Two hybrids used every year, Aréna/PR and Alexandra/PR hybrids were tested by Kang’s stability analysis. We found that Alexandra/PR was most balanced at every levels of crop density. Both hybrids performed most stable yield at 65000 ha-1 crop density level and less balanced at 35000 ha-1 crop density level. As a result of improved environmental conditions, the yield increase of Aréna/PR was higher than that of Alexandra/PR.
Our regression analysis found that the maximum yield of Lympil, Louidor, Hysun 321, PR63A82 and PR64A63 hybrids were harvested at 47000-60000 plant ha-1 crop density level. The statistical analysis showed that the highest yield was harvested from Lympil and Hysun 321. As regards the crop yield, the most stable hybrids were Louidor and Lympil. The optimum crop density interval of Rigasol/PR and Larisol (58000 plant ha-1) was wider than that of Diabolo (46000 plant ha-1). The maximum yield of Larisol was higher at the optimal crop density level. As regards yield, Diabol was the most stable hybrid.
The statistical analysis on the stability of the yield of Alexandra/PR and Aréna/PR showed that Aréna/PR is more stable, and its optimal crop density level is lower than that of Alexandra/PR. -
A simple method for preparing elemental selenium nano- coating inside a silicone surface
35-43Views:534Selenium nanoparticles (SeNPs) with a bright red colour have aroused worldwide attention due to their unique properties in selenium supplementation because of their low toxicity and favourable bioavailability. A simple method was developed for making a red selenium nanolayer on the inner surface of Polyvinyl chloride (PVC) and silicone tube. The selenium nanoparticles were produced by the reaction of sodium selenite and ascorbic acid. Red amorphous selenium nanoparticles have been successfully synthesized by the reaction of 500 mg dm-3 Se (sodium selenite) solution with 10 g dm-3 ascorbic acid solution at room temperature, and morphology was confirmed by X-ray diffraction analysis (XRD). The coating density was compared on PVC and silicone surfaces by using Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray (EDS) analysis. The nanolayer with about 16 µm thickness on the silicone surface significantly evenly distributed compared to the PVC surface. The selenium coated silicone tube could be a good source of selenium for a continuous, low-level selenium supplementation of farm animals via drinking water.
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Investigation of the Resistance Against the Fusarium solani at Different Pea Varieties and Combinations of Breeding
157-160Views:126The Fusarium species are soil and polyphage parasits, and the rate of damage, caused by them, highly depend on interactions between climatic and edaphic factors and also on sensitivity of cultivars. Even though about 70-80 percent of the widely grown green peas cultivars is resistant to Fusarium oxysporum f. sp. pisi 1. race, the rate of Fusarium infections and severity of symptoms increased in the latest years. It is supposed that another Fusarium sp. the Fusarium solani has been spreading. The most exact way to study the cultivars in a provoking garden established in natural environment, where the pathogen is artificially enriched to a level, at which the cultivars can be distinguished according to their susceptibility. In the provoking garden the reaction against to Fusarium of our breeding lines and our registered cultivars and cultivars existed on the National List (including cultivars with well-known susceptibility as standards) are examined year by year. In our experiments we could found two green peas cultivars to be resistant to Fusarium solani (Early sweet (13,36%) and Lora (16,9%)) The breeding lines Margit and 8607/75-3-2 proved to be the most susceptible to Fusarium solani (94,4% and 73,1% infected plants, respectively).
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The time, than the resource of the managerial activity
19-24Views:154The role of the time factor in management is increasing. Change directs attention to the time factor from the point of view of the organisation, and management does the same from that of
the person. I examined the time management practice of managers of organisations related to agribusiness, and the reaction time characterising the adaption ability of the organisations through a questionnaire survey. I analysed the collected data with scientifically established statistical methods.
During studying resources I analysed the time factor from two aspects. On the one hand, I wanted to know what role time has in managers’ opinions in increasing company efficiency and
performance. On the other hand, I asked them how much they consider this resource to be expandable. -
Detection of DNA mutations by PCR-TTGE method
21-25Views:503In our study PCR-temporal temperature gelelectrophoresis (TTGE) and MeltINGENY bioinformatic program were used to analyse the mutations in the genes of melanocortin-1 receptor (MC1R) and pituitary adenylate-cyclase activating polypeptide (PACAP) in cattle. Amplification of target DNA by PCR was performed with GC-clamp primers and non-GC-clamp primers in simplex PCR reactions. The fragments were separated by denaturing polyacrylamide gelelectrophoresis (denaturing agents: high temperature, urea) after PCR reactions.MC1R homozygous individuals were used for the reaction.
We concluded that MeltINGENY program makes the decision and detection system easier, and more simple as the melting profile of target sequence is determined by the software. In case of MC1R gene, PCR-TTGE method is appropriate for SNP detection, however PACAP gene polymorphism can not be identified by the method, because PACAP mutations are not included in melting domains, therefore PCR-TTGE cannot detect them.
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Mitigation of the effect of secondary salinization by micro soil conditioning
115-119Views:397This research has the general goal to meet the customization of agriculture in small scale farming. We are developing a technique using micro doses of soil conditioners and organic material applied in the root zone of vegetable crops. We expected to change the physical and chemical properties of the affected soil, which has been irrigated with salty water. Two different soil conditioners were tested. A lysimeter experiment including 8 simple drainage lysimeters was set up in the Research Institute of Karcag IAREF University of Debrecen in 2017. The main goal was to study the effect of different soil conditioners on the soil endangered by secondary salinization induced by irrigation with saline water. In order to compare the difference between the treatments, we collected soil samples, water samples, and determined the yields. Chili pepper (Capsicum annuum) was used as an indicator crop during one specific agricultural season. The technique called micro soil conditioning is rational because several reasons. The roles of the technique are various, for example it can serve as a source of carbon or a container for soil amendments and can minimize evaporation. We found this technique not to interfere with the chemical reaction or the interaction with the plants. However, the micro doses of soil amendments had the role to minimize the risk of soil degradation and do not significantly influence soil respiration. In addition, by improving soil properties, soil conditioning increases the leaching of the excess of salts from the root zone. In fact, this technique can decrease the cost of the inputs and improves the production of vegetables, and at the same time mitigates the effect of secondary salinization.
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The effect of plant density to the yield results and the yield components of maize hybrids
89-93Views:273Maize is the crop that is produced on the second largest area in our country, in Hungary. It is planted on nearly 25% of the country’s growing area and it was produced on 1 090 439 hectares in 2016. Despite the continuous development of the biological basis and production
technology, the growth of the yield results is not constant, its fluctuation is significant. It can be even up to 60%, because of the extremity of the years. The exploitation of the yield potential of modern hybrids is possible if we harmonize the effects of the ecological factors and properly applied instruments of agro technology and by these we ensure their interaction to reach a favorable outcome. The applied plant density is an important, well researched, but at industrial level a not enough utilized element of the maize production.
The results of the extensive tests, done between 2009 and 2015, showed that the genotype, the year effect and the plant density are in strong correlation with each other determining the yield results. In the past seven years the examined genotypes reached the highest yield
performance at the highest plant densities. The early hybrids (RM90–95, FAO 200–300) are capable of producing them at higher plant density, while in case of the mid and late maturity varieties the further increasing of the density after reaching the optimum level led to yield depression.
According to our experimental results, the yield is in close positive correlation with the increase of the plant density. The effect of the growing season has great significance in forming the yield results and this determines the applicable plant density too.
The yield of maize is determined by a resultant of components. The main component is the number of ears per plant and the amount of kernels per ear, which is calculated from the number of kernels on an ear and the weight of them. The number of the kernels on an ear is
calculated from the number of rows on the cob multiplied by the number of seeds in one row on the cob. In dry years, at lower yield levels the yield decreases because of the shorter ears, while at the higher levels the number of kernels in a row and the thousand-kernel weight decreases,causing yield depression this way. From our examinations it turned out that the plant density reaction of a genotype is individual, every variety reaches its maximum kernel number per hectare – in other words the maximum yield - in an individual way. -
Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary
193-200Views:310The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.
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Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds
99-104Views:220Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant.
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Examining reaction of sunflower genotypes on planting time on chernozem soil
93-99Views:247We studied the effect of planting time on plant pathological factors, leaf area index and yield production by applying various fungicid treatments on two different sunflower genotypes in 2013.
By delaying planting time, both the extent of Diaporthe, Alternaria and Phoma infections decreased. The differences between the volume of infections were significant in the case of the early and late sowing time results. The application of fungicide treatments induced a notable decrease in the extent of infections for all three pathogens examined. The LAI-values varied between 0.3 and 5.6 m2/m2 in 2013 depending on the hybrid, sowing time and treatment. Stocks planted at distinct times reached maximum leaf coverage at different times. The planting time and the fungicide treatment had a significant effect on the formation of the leaf area. In the case of average and late planting times, fungicide treatments elongated the preservation of the green leaf area.
With respect to the yield amount, average planting time (27 April, 2013) turned out to be optimal in 2013 (control – NK Ferti: 4.621 kg ha-1, PR64H42: 4.196 kg ha-1; double-treated: NK Ferti: 5.282 kg ha-1, PR64H42: 5.090 kg ha-1). Fungicide treatments resulted in significant yield growth in all cases during our research.
We applied Person correlation analysis to evaluate the hybrids’ sensibility to infections and our results varied in the case of Diaporthe and Phoma (r=-0.343*, -0.379**). Infections of the three pathogens were significantly reduced by delaying the planting time and applying fungicides. Late sown stocks preserved the green leaf area for a longer period. Besides, the application of the fungicide treatment and the hybrid itself also led to the preservation of the green leaf area. However, pathogens examined notably decreased the leaf area by the end of the growing year. The fungicide treatment had a remarkable effect on yield growth (r=0.603**). Furthermore, the presence of higher LAI-values in the period prior to August also induced higher yields.
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Does drought stress always negatively affect the yield and quality of soybean in Hungary?
37-40Views:433Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.
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Technological development of sustainable maize production
83-88Views:294In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize.
The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly eight ha field. The size of one plot was 206 m2, this it was a half-industrial experiment. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. Yield increasing effect of the fertilizer also depended on the number of plants per hectare at a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants per ha.
In Hajdúszoboszló, in 2016 the amount of rainfall from January to October was 605 mm, which was more than the average of 30 years by 160 mm. The yield of hybrids without fertilization changed between 9.63–11.6 t ha-1 depending on the number of plants.
The six tested hybrids is 10.65 t ha-1 in the average of the stand density of 60, 70 and 80 thousand plants per hectare without fertilization, while it is 12.24 t ha-1 with N80+PK fertilizer treatment. That increase in the yield is 1.6 t ha-1, it is significant.
Da Sonka hybrid is sensitive to weather, it is able to produce 6 t ha-1 additional yield in case of favourable condition. However, it has a low stress tolerance. The most stable yields were observed at Kamaria and Pioneer hybrids. The effect of vintage is also an important factor on the yield. In average, the yield of maize was 6.81 t ha-1 in 2015, which was a drought year and 11.86 t ha-1 in 2016 that was a favourable year. -
The effect of season and fertilizer on the LAI, the photosynthesis and the yield of the maize hybrids with different genetic characteristics
27-34Views:339The experiment was carried out in Debrecen, at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization. The three factors of production technology jointly determine the successfully of maize production, but in different measure. The yield and the stability of yield of maize can be increased with hybrid-specific technologies.
In 2004-2005 experiment years the favorable results reached were due to the rainy season. There were significant difference between the productivity of maize hybrids. The N 40, P2O5 25, K2O 30 kg/ha treatment caused the highest increase of yield (3-5 t/ha) compared to the control (parcels without fertilization). The reaction of hybrids to the further fertilizer doses was different. The agro-ecological optimum of NPK fertilization was N 120, P 75, K 90 kg of the most hybrids.
During the experiment, we tested the moisture loss of the five hybrids. The seed moisture content at harvest was higher than in previous years due to the rainy seasons. The seed moisture content of harvest of FAO 200-300 hybrids were about 20%. It changed between 21-24% in the case of hybrids with longer vegetation period (FAO 400), the seed moisture content of Mv Vilma (FAO 510) was 24.21-25.04% in the average of fertilizer treatments. There is an important difference between the moisture loss ability of hybrids which changed 0.2-0.6%/day. The moisture loss of hybrids changed depending on the fertilizer treatment; usually, it was more favorable in the optimal fertilizer dose (N120+PK).
In the case of tested hybrids, we measured the highest LAI and photosynthetic activity at the optimal treatment, N 120, P2O5 75, K2O 90 kg/ha in the respect of efficiency and environmental protection, and the yield was high also for this treatment. There are significant difference between the LAI, the photosynthetic activity and the yield of hybrids, and these values could change depending on the treatment of fertilization.