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Analytical overview of dairy industry in the Poltava region, Ukraine
47-51Views:122The author presents essential characteristics of the state and place of Poltava region in it. The study aims at identification and description of latest trends in Ukraine’s and Poltava’s dairy industry. The author stresses an important place of the dairy industry in the agriculture of Ukraine, and particularly in the Poltava region. This article also presents the information of main indicators of dairy industry of Ukraine in the comparison with other countries of the World. It describe sources of forming the main agricultural products and directions of their use for the calendar year, data on per capita consumption of main dairy products in Ukraine and the Poltava Region and per capita average daily consumption of basic micro- and macro elements in foods. The study contains basic indicators which characterize the social and economic state of agriculture in Ukraine and its regions for 1990–2012. There are also the data of gross production and main kinds of dairy products per capita and as well as some indicators of livestock’s productivity and so on.
The goal of the article is to describe the complex evaluation of the achieved level of milk production, consumption of milk and dairy products in Ukraine and the Poltava Region, to show the specifics of the national dairy cattle breeding, identifying the main challenges in it.
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The Fattening and Slaughtering Examination at the Hungarian Racka Sheep
37-42Views:87Fattening and slaughtering characteristics of both types (black and white) of the Hungarian racka sheep were investigated at the site of the National Institute for Agricultural Quality Control in Atkár. The Hungarian merino, the dairy cigaja and the British milksheep were applied as control groups. 10 animals were examined from both sexes in each genotype. The examination was carried out according to the principles set in the Codex for sheep breeding. During the test acute diarrhoea occurred in each control group. However, only few lambs were excluded from the test from the British milksheep and the Hungarian merino groups because of significant changes in their condition. Consequently, the results obtained were not significantly influenced. No symptoms of any illness could be recognised in the Hungarian racka sheep, though. The significance of the difference between the average of the examined groups were checked by T-tests.
Daily gain was significantly less compared to the control groups, except for the white racka males. The lambs starving for 24 hours before slaughtering weighed 25.3-30.0 kgs on average. The black and white racka females weighed the least while the Hungarian merino females weighed the most. The quantity of abdominal and kidney fat was significant in the case of our native females. The quantity of abdominal fat was significantly bigger compared to each control group. On comparing the investigated groups it turned out that the white racka males had the biggest skin weight while the white Hungarian racka females had the smallest, that is the two extremes were recognised in the same colour (white) of one bred. On qualifying the slaughtered animals females had better results than males in each bred; the racka sheep were better than the dairy cigaja but were worse than the Hungarian merino and British sheep groups.
The huge differences in the carcasses of the groups were seen clearly when chopping. Examining the leg, it was noticed that the native groups proved to be smaller than the control groups, except for the black racka males. As for the short loin, the Hungarian racka and the Hungarian merino sheep proved to be the best, whereas the dairy cigaja and the British milk males had much smaller ones. The results gained when investigating the rack were similar to those of the short loin. As far as the rib and shoulder are concerned, the black racka females and the dairy cigaja females had the worst results. After chopping, the right side was also boned in every group. The small proportion of bone in the case of racka sheep was easily noticeable that resulted in the fact that the white racka females had the highest quantity of short loin compared to the other groups. When examining the back quarter, the bone proportions of the 4 racka groups proved to be significantly less than those of the control groups but it was not true for the meat proportions. As for the front quarter meat, the racka males were prominent in the great shoulder meat and in the small proportion of bone. The quantity of the first quarter bone was the least in the case of racka sheep and was the biggest for dairy cigajas. This difference proved to be significant in most cases.
In short, the Hungarian racka groups had the best results in meat quantity, which was especially significant compared to the dairy cigaja sheep. However, the quantity of external fat increased. -
Investment funding programs on dairy farms in Hajdú-Bihar County
11-16Views:166The dairy farming sector needs constant modernization of its buildings, machines and technology in order to maintain its competitiveness and productivity. For the greater scale investments, these companies try to apply for state funds in addition to their own financial sources. Therefore, the investment support system plays a great role by improving the competitiveness and modernity of these farms. In the last 10 years, 7 funding programs were available for dairy farms. In this research, 25 dairy farms were interviewed in Hajdú-Bihar County. The examined farms cover more than 50% of the milk production in Hajdú-Bihar County. The survey questions focused on the investment funding program use of farms between 2007 and 2017 and renewable energy investments including all programs. The results show how many tenders were submitted, how many were successfully evaluated and completed. All farms had at least two successfully evaluated projects. A correlation was found between completed projects by farm size and completed projects. The connection of the modernity index of dairy farms and the completed projects was also shown.
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Analyzing the Efficiency of Dairy Farms by Using the Method of Data Envelopment Analysis (DEA)
17-21Views:88In Hungary the dairy sector is in a long-term critical period, the stock has been in constant decline. The consumption of milk and dairy products in Hungary is slightly rising compared to the world tendency, and it is fallen behind the level in 1990. The milk consumption per capita in 2006 was with 75 liters less than the EU-15 average. Dairy enterprise is a very risky activity: the profitability of the enterprise is affected by the fluctuation of feed and animal health products prices from the side of inputs, and by the fluctuation of end-product prices. Under these circumstances it is vital for the
cattle breeders, in order to survive, to harness the reserves in the breeding as effectively as possible. In our research we made a multi-faceted efficiency analysis of an agricultural holding’s three dairy farms. The chosen method for the analysis was Data Envelopment Analysis (DEA). The
selection of the method is justified by the fact that there is not such a reliable database by which we could define production functions, and that DEA makes possible to manage several inputs and outputs, i.e. multiple decision problems, simultaneously. By using DEA the sources that causes shortfalls can be identified, analyzed and quantified on farms that does not operate efficiently, thus it can help the corporate decision support successfully. In the model inputs are the cost data per one liter milk – feed, medicinal product use, logistic costs -, and the main parameters
concerning the keeping and rearing. Outputs are indicators concerning milk production, milk quality and others. We prepared the model in MS Excel, the linear programming model series were programmed by Visual Basic. After solving the model, in light of the shadow prices we can determine why either of the farms is not efficient. -
Analysis of vitamin E content in pilot dairy products
31-34Views:115Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount. -
Analysis of vitamin E content in pilot dairy products
49-52Views:158Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount.
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Study of animal welfare status and heat stress measures applied in dairy cow herds in Hungary
79-82Views:91The following material focuses on dairy production and climate related issues in Hungary. All the data was gathered during PhD
project: Study of animal welfare status in dairy cow herds in Hungary. Relations between animal welfare and climate changes expressed by
increase in temperature are described. Extremely hot weather creates hard conditions for milking cows when animal welfare is highly
compromised. From the preliminary results obtained one might formulate hypothesis that there are still areas on the farms where immediate
actions should be taken to give a relief to cows in hot seasons. There was found significant number of farms with too many animals per one
water trough, dirty water troughs, limited access to water troughs and hazardous surface for cows in critical places where many animals are
gathered. Calves with not sufficient amount of water in hot days and other parts of the year were reported. Silage exposure to the sun and
mouldy food in a silage clump was also found to be an important factor in monitoring impact of warm weather. Half of the farms letting
animals to spend time on the pasture or paddock did not provide shade for animals. Low conception rate of first insemination was predicted
to be influenced by heat stress, what is proved by lack of heat decreasing measures taken on the farms. -
Effect of the claw disorders on production performance in dairy cattle
15-19Views:158The aim of the study was the influence of claw disorders on production traits in dairy cattle. Observed were claw traits as claw angle, claw length, heel index, claw height, claw diagonal and claw width. Right hind claw after functional trimming was evaluated. Occurrence of claw disorders like interdigital dermatitis and heel erosion (IDHE), digital dermatitis (DD) and sole ulcer (SV) as well as the progress of disease were observed. Basic summary and variation statistics was performed by the SAS software. Two herds were included in the study. Holstein dairy cows (n=101) produced 11 875 kg of milk, 468 kg (3.94%) of fat and 396 kg (3.34%) of proteins. Holstein cows were affected with IDHE in 22 cases, with DD in 6 cases and with sole ulcer in 10 cases. Slovak Simmental dairy cows (n=101) produced 5834 kg of milk, 258 kg (4.44%) of fat and 209 kg (3.59%) of proteins. Slovak Simmental cows were affected with IDHE in 8 cases, with DD in 5 cases and with sole ulcer in 19 cases. Observed was that claw disorders caused deviations of milk production.
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Complex problem analysis of the Hungarian milk product chain
43-47Views:90Hungarian dairy sector went through significant changes in past two decades. The most significant changes were caused by our accession to the European Union. In Hungary milk production remarkably declined after EU accession. The size of our dairy herd has been practically reducing since the political transformation, but increasing yields per cow could compensate it in some way and for some time. However, in recent years, increasing yield per cow came to a stop and in parallel, the number of cows declined further and faster. Low prices, high production costs and tightening quality requirements ousted several producers from the market in past years. Feeding cost represents the highest rate in cost structure of production, but animal health expenditures and various losses are also significant. There are undeniably competitive disadvantages in the level of organisation and labour productivity; however competitiveness already depends on cost effectiveness in the medium run. In Hungary concentration of the dairies is relatively strong in spite of the relative high number of corporations. The dairies compete with each other and with the export market for the raw material and the better exploitation of their capacities. Applied technology of the Hungarian dairies lags behind the Western-European competitors’; in addition they have handicaps in efficiency and product innovation. Presence of chain of stores being dominant in sale of milk products does also not favour in all respects to the position of the dairies. The aforementioned retail chains are namely consumer-centric, engage in price follower conduct and weaken the position of the dairies with their private label products. As a result of increasing import of milk and milk products Hungary became a net importer in recent years. Today, disposable income still essentially determines the consumption habits of price-sensitive consumers. Loyalty for Hungarian products is not typical, consumers are open for import products being preferred by retail chains. In addition Hungarian milk and milk product consumption is about half of the Union average and it is far behind the level being necessary for healthy eating. In Hungary lack of competitiveness and vertical integration relationships and backwardness are revealing among the dairy farmers and the dairies, while chain of stores are in unprecedented “monopolistic situation”; the whole sector can be characterised by defencelessness.
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Microbial assessment of potential functional dairy products with added dried herbs
59-63Views:114The market of dairy products is a dynamically developing sector of the food industry. Potential, functional dairy products, made by adding herbs or spices, will have antimicrobial and antioxidant effect due to the active biochemical agents of the plant additives. Furthermore, these active components will widen the storage life of food products and enhance their organoleptic properties too. We worked out a technology for creating fresh cheeses using a gentle pasteurizing method by treating the mixture of raw milk and 1.5% fat contained in commercial milk. As herb additives, we used citronella (Melissa officinalis), and peppermint (Mentha x piperita) harvested by us and dried them via Tyndall-method in convective dryer on 40 °C for 5.5 hours per day. The drying period took three days. We bought dried citronella and mint from the supermarket, which were dried by ionizing radiation, to compare the microbiological pollution with the herbs dried by us.
The main target of this research was to create a microbiologically stable, potential functional dairy product. However, because of the bad quality of the raw milk and the gentle heat treatment we used for sterilizing bulk milk, or else, cheeses were not safe for human consumption. As a consequence, we need further studies to modify our technology and get a microbiologically stable product.
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Inner pelvic measurements in dairy breeds
31-35Views:97Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons done on dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jerseys turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshire, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the apparent ideal 1:1 horizontal and vertical diameter ratio; however, this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Although this dairy breed is the lightest, when the pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
Effect of vitamin e and selenium supplementation on the antioxidant content of milk and dairy products in dairy cows
9-12Views:113In 2007, the aim of the Ányos Jedlik program and the call for tenders was to support application-oriented, strategic research and development projects, which can increase the competitiveness of the Hungarian economy. In the framework of our project, we intended to examine whether non-protected antioxidants - in this case: vitamin E and selenium – used as feed-additives can increase the antioxidant content of milk. The milk with an increased level of vitamin E and selenium content can be used for producing functional foods which will represent competitive products on the current market of milk products. Our results show that the use of vitamin E and selenium as feedadditives can significantly increase the amount of vitamin E and selenium in the milk and also in the diary products.
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Inner pelvic measurements in dairy breeds
51-56Views:108Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons in dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jersey turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshires, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the seemingly ideal 1:1 horizontal and vertical diameter ratio; however this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Though this dairy breed is the lightest, when pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
The role of type traits of dairy cattles in long productive life
11-18Views:92In the last few decades, a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Additionally, the reproduction biology of the population was adversely affected by keeping and feeding technologies. Productive lifetime has shortened and the service period has lengthened. However, profitable milk production requires cows with longer productive lives and larger life-time productions. In our study, we made a comparison between production, culling causes, and type traits of culled Holstein Friesian cows after first calving and of cows with a minimum of eight lactations. We established that the first lactation production of cows with longer productive lives was more or less homogeneous. Disease of metabolism as well as digestive and respiratory problems, were the main causes of the culling of cows with short productive lives, and reproduction and udder problems as well as low production, were the main causes of the culling of cows with long productive lives. We found a significant difference in the distributions of strength, body depth, dairy form, croup width, rear leg side view, foot angle, fore udder attachment and teat placement between the two groups. Furthermore, we established that cows with lower stature, less strength, a somewhat deeper body, better dairy form, narrower croup width, smaller foot angle, looser fore udder attachment and some outside teat placement had longer productive lives.
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Labour Efficiency of Dairy Farms with Different Types and Sizes in Hajdú-Bihar County
62-67Views:75Questions and uncertainties characterised the accession period of Hungary to the European Union. It is evident that only those producers can compete on the market who produce good quality products. Therefore, only dairy farms with extra high quality raw milk production will be successful. Furthermore, on this basis, rural areas will be able to keep their inhabitants one of the most important problems presently. The author examined the process of milk production and the labour efficiency of 18 dairy farms in Hajdú-Bihar County. Mechanization, the quality of the work force and the system of incentives were assessed. Data of produced milk quality were collected too. Because of farms with different types and sizes the circumstances of milk production differ greatly. As a result of data analysis it was found that the working schedule of small and large farms often differed. The most important working processes (milking, feeding, harvesting) are done by family members. In order to increase the efficiency and improve working atmosphere the improvement of wage and incentive systems would be desirable. Labour efficiency indexes show that the efficiency of tie-stall systems is lower than the efficiency of similar size loose-housing systems. It can also be stated; that labour efficiency of similar size loose housing dairies differs greatly so the importance of work-studies should be increased.
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Data supporting the quality of sheep milk for processing
67-73Views:82Although the volume of ingredients in ewe’s milk is substantially higher than in cow’s milk, its hygienic quality is lower. The weak quality of raw ewe’s milk limits the possibilities of processing and results in bad quality products. In our investigation we analysed the state of ewe’s milk processing at a typical medium size dairy firm. We investigated the collection, the amount and the quality of milk and the level of ingredients in milk throughout the purchasing period (lactation period).
The purchasing of ewe’s milk was limited to 5 months (from April to September). Although meat (lamb) provides the major source of income to sheep breeders the extension of the period of ewe’s milk production can be beneficial to shepherds and dairy firms. The amount of ewe’s milk ingredients found corresponded to published findings. However, the hygienic quality of ewe’s milk was varied greatly in the different milk samples and these deviations meant bad quality on average. Physiological factors, the circumstances of sheep breeding and milking, the slow cooling of the milk, the little amount of daily milk and the long storage before transportation to the dairy firm together cause poor hygienic quality. The main problem is the long storage time of milk, but our results raise the question of reconsidering the quality classes. Investigating the effect of the hygienic quality of raw milk on product quality, we can get correct data that can be really authoritative. -
Examination of the presence and effect of udder edema in Holstein-Friesian cattle
125-130Views:104Mastitis is the most common disease of dairy cows and the most expensive in terms of treatment. For this reason, great emphasis must be placed on its prevention. Based on literature sources, it can be said that there is a positive correlation between the prevalence of udder edema and the occurrence of mastitis. Our studies were carried out in a dairy farm in Csongrád-Csanád county. So far we have collected data from 64 cows with udder edema. The severity of udder edema was evaluated on a scale of 0–3 points one week before calving and for 3 weeks after calving. We observed that udder edema occurred in 78% of the studied cows at the time of the first calving. After calving, the length and diameter of the four udder teats were examined. Both anterior and posterior teats were shortened in severely edematous cows. When examining the diameter of the teat, we found that, depending on the severity of the edema, the anterior and posterior udder teats were 2.5 and 1.6 and 1.6 and 1.5 mm thicker respectively than those of cows without edema.
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Evaluation of Dairy Farms’ Competitiveness
256-260Views:32The volume of milk production in the European Union is limited by quotas, thus dairy farms in Hungary can expand their production mostly at the others’ expenses after joining. In this way issues of arrangements arise relating to defining competitiveness of the already existing farms and not to farming new ones. The aims of my research is to find answers for dairy farms in the County of Hajdu-Bihar, depending on their arrangement (herd size, keeping technology, arable for forage production, handling manure, mechanisation, technician state of equipment) what possibilities they will have among the EU farmers.
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Some Aspects of the Relationship Between the Quality of Sheep Milk and Dairy Products
12-15Views:84The correlation between the quality of raw milk and the quality of milk products is evident. In spite of this fact we can find only a few references contained exact data related sheep milk. In recent paper we investigated the effect of SCC of sheep milk on the cheese yield (semi hard traditional cheese) and certain texture parameters of yoghurt from sheep milk. We wanted to know the relevant limit values of these properties for dairy applications.
In our opinion – in the case of the cheese yield – that the strong negative effect can be experienced when SCC is above 700.000-1 million/cm3.
In the case of yoghurt from sheep milk the limit values of SCC can be 1 million/cm3 for Adhesivity and Whey draining and 500.000/cm3 for Hardness. Considering these limit values in the selection of milk become materialise the highest quality of products and the economical production. -
Production of novel fermented milks
303-305Views:170The objective of this research was to test the influence of various natural substances on acid production, growth, and viability of characteristic microorganisms in yogurt and probiotic fermented dairy foods. Oligofructose, inulin, honey, and the dried biomass of Spirulina (Arthrospira) platensis were found to stimulate the growth rate and acid production activity of the major thermophilic diary cultures tested and, in addition to this, the presence of the aforementioned substrates also improved the survival of starter bacteria in fermented milk products during storage. The reduced production time of cultured milks resulted in increased production efficiency. The stimulatory and/or protective effect of oligofructose,
inulin, honey, and Spirulina on Bifidobacterium spp. is probably the most important finding of this study because bifidobacteria do not grow well in milk and they have low survival rates in conventional fermented milks. Some of the bioactive substances tested were also capable of exerting an antifungal effect on spoilage yeasts and molds, and improving the nutritional and sensory properties of the final product, thus providing a new opportunity for manufacture of functional fermented dairy foods. -
Identification of the Slovak traditional cheese “Parenica” microflora
227-239Views:230Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.
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Concentration and Horizontal Integration in Milk Production
60-66Views:87Agriculture in Hungary was characterised by the duality of large-scale farms (co-ops and state farms) and small-scale private enterprises until the beginning of the 1990s. Due to the privatisation and transformation of co-ops farm structure has significantly changed. The transformation of the structure is not considered to be a completed process. Level of concentration and integration on private farms are rising, new co-operation forms are appearing and new types of enterprises and producers’ groups are being formed.
Concentration and integration have great importance in the dairy sector among agricultural enterprises. In recent years, milk production in the European Union has been characterised by concentration, leading to greater competitiveness. Today, most dairy producers belong to different kinds of producers’ organisations as concentrated demand may only be competitive by meeting concentrated supply. In Hungary, the problems of production have been the fragmentation and decrease of the average farm size while, at the same time, concentration has occurred in the processing sector. Establishing producers’ groups may be one of the solutions for improving competitiveness production. -
Identification of cow’s and buffalo’s milk and dairy product using a DNA-based method
279-282Views:138Aim of our study was the optimization of a DNA method, that is appropriate for reliable, low cost identification of animal species in milk and dairy product (cheese) and to determine the ratio of species. Mitochondrial DNA was used in our work to analyse buffalo/cow milk mixtures contained different ratio of bovine milk such as 0.1%, 0.5%, 1%, 1.5%, 2%, 5%, 10%, 15% (v/v%). Buffalo cheese were produced using buffalo and cows milk (0%, 2%, 5%, 10%, 15% – v/v% cows milk in buffalo milk). In case of milk mixtures, using species specific primers, the PCR assay showed a 0.5 v/v% detection limit. Cattle, in the buffalo/cows milk 99.9/0.1 v/v% mixture, was not detectable. The identification of buffalo and cows DNA in cheese was successful. The intensity of eletroforetic PCR fragment indicated the increase of cow milk ratio in milk and cheese samples as well.
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Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:108Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
25-29Views:116In the last 20-30 years, lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimizing lameness. Those areas are related to poor facilities, lack of straw, problems with labor and basic management.