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Examination of the current and idel organisational culture and leadership elements at the municipalities of the North Great Plain region
117-125Views:126The connection between organizational culture and leadership has been examined by several researchers (Schein, Schmircik, Bass) and it is proven that there is a link between them. The leader shapes the organizational culture and at the same time the organizational culture shapes the leader too. The middle managers of local governments place the major emphasis on the dimension of goal orientation for the future. From the leadership perspective they find charismatic, goal and team oriented leadership necessary for the future.
The local governments have to answer the challenges of the rapidly changing environment. Quick responses and adjustments are only possible if the leader possesses a clear future vision and not only sets short-term goals but plans for the future and estimates the necessities on the long run. It is important to have a leadership with utmost dedication to the organization and to the objectives of the organization. The leaders must raise the interests of the employees, involve them in the process of setting goals and in finding ways to meet those goals, and that the employees should no longer strive to realize their own personal ambitions but focus on the common objectives. This brought transformational leadership to light.
The leader establishes and shapes the organizational culture but the individuals and teams working for the organization have impact on the organizational culture as well. This becomes apparent in the organizational culture as middle managers would place the major emphasis on performance orientation. Performance orientation is a dominant motivation based on excellence, hard work, pre-calculated risk, fore planning, goal orientation and regular feedback, which shapes the leadership too, as the leader has to change as well, in order to run the organization.
Scheins’ standpoint reflects the best the relationship between the organizational culture and the leadership. Schein claims that organizational culture and leadership are interwoven phenomena, as the leader shapes the culture but after a while the organizational culture itself shapes the leader too.
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The relationship between the organizational culture and the individual attitude, particulary the question of individualism and collectivism
165-170Views:84There is considerable consensus regarding the development of organizational culture; namely, that culture is the result of socialization, evolving through a social learning process. However, the mechanism of the process itself, along with the factors influencing it, raises a number of questions that are researched by different schools of thought. Some of the contextual factors that affect the development of culture, such as the characteristics of national culture or charismatic and other dominant people as role models, seem to be quite influential, especially when other influential factors affect these as well. Value analysis can be helpful for understanding the essence of organizational culture, and it can reveal underlying, hidden phenomena. The results of these studies depict organizational culture as a list of values or as a hierarchy. Values carry emotions as well, that may refer to their link with attitudes and to the fact that the two can be mixed up. The latest innovations in action and thought were the results of the political changes of the 1990s whose implications can be identified in both individual and national values. Changes of views and attitudes also emerge in the changes of collectivist-individualistic thinking and values which become part of the organizational culture and value structure according to value-centered analyses. The changing values define and influence our own opinions, identity, and, in turn, our behavior. Values reflected by our behavior and actions are to be guided by the harmony of individual, organizational, and national value systems. The lack of this synergy results in the dissonance of contrastive values and blocks actions. This, in turn, interferes with individual productivity and thus slows down organizational productivity as well. Our goal should be the synergy of values which creates the harmony of individual thinking and behavior since this harmony is the basis of success and productivity.
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Possibilities of conceptualization and operacioanlizaton of the safety culture and safety climate in the international safety management researches
117-126Views:54In this paper the author summarized the definitions and operationalisations of the safety culture and safety climate concepts. He analyzed the Hungarian and international safety
management scientific literatures. He also analyzed the scientific definitions and summarised the common and different elements. The author emphasizes that the many different scientific safety culture researches had been created good possibilities to find the common surfaces. Based on the international safety culture operationalisation practices the author identified a composition of safety culture dimensions which could be a possible basis for the Hungarian safety culture researches. These dimensions are:
???? values (individual and organizational) from this result scale of values,
???? attitudes (individual and organizational),
???? motivations,
???? perceptions,
???? social status and estimation of the occupational safety and health,
???? position and estimation of the occupational safety and health communication,
???? position and estimation of the occupational safety and health tranings,
???? measuring of confidence, optimism, fatalism and anomic characteristics,
???? good practices and behavior,
???? influence and estimation of the work,
???? risk perception and safety level perception,
???? contentment.with occupational safety and health. -
The possibilities and framework of conditions of organisational development at a particular administrative body
63-70Views:109The demand for the recognition, identification and introduction of measures aimed at improving organizational culture and the efficiency of management and leadership plays a crucial role in the life of every organization, including the law enforcement bodies as well. The tasks and responsibilities of the Hungarian Police Force have changed considerably both in terms of quantity and quality since Hungary’s accession into the European Union first, and since the country’s joining the Schengen Zone. We are now facing problems formerly unheard of (e. g. the integration of the police force and the border guard, joining the Schengen Zone, the emergence of new forms of crime). As border control within the Union stopped, organized crime has become more powerful. The changes are by no means over: in order to improve the subjective sense of security of the population and to advance organizational efficiency, a number of changes are being implemented at present. For the internal security of the member states of the Union and for the improvement of the efficiency of police work in the member states, the use of consciously coordinated management methods is indispensable. These efforts are the subject of this essay.
A police force is an partially open system, the work of which is influenced by a number of external processes and factors. The organizational culture of a police force is determined by its traditions on the one hand, and by the demands to develop its functions as an authority and a service provider on the other. It is undeniable that any conscious effort in shaping and developing the organizational culture of the police requires special competences and commitment from the leaders of the organization. Furthermore, it is also necessary to modernize the management tasks. It applies to all the levels of leadership, from the head of a patrol to the supreme commander of the police force.
The organizational scheme of the police force is that of a functional, linear system. Such an organization is characterized by a relatively high degree of inflexibility, a slow and cumbersome reaction to external changes. All these may adversely influence organizational efficiency. It therefore appears to be necessary to examine the present structure of police, and survey the possibilities of modernization. Modernization does not necessarily mean a change in the structure; it may be restricted to changes in organizational culture, the creation of an atmosphere favourable for reforms, and the coordination of diversified structures. In this paper I wish to demonstrate some of the possibilities of improving organizational culture and organization development, based upon the findings of a specific survey.
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Coherence and connection between the good pond culture practice and the environment conscious management
60-73Views:73According to the data indicating the decline and restructuring during the past decade, as well as the trend in the European Union member states, it can be expected that the role of traditional agriculture and fish production in direct rural employment decrease further. This also values those strategic directions for restructuring that will lead fishculture from quantity driven to quality production along with sustainable development (i.e. environmental conscious production) and multifunctional farming. This way the economic and social tensions caused by the concentration of the production and labour output can be mitigated.
It is laid in the 1257/1999 Act on rural Development that farmers that enrol the agri-environmental scheme should follow the “Good Agricultural Practice” on the whole managed area. In case of agri-environmental schemes this is a precondition for which no grants are given.
The adaptation of “Good Agricultural Practice” in fishproduction,where it is called: “Good Pond Culture Practice” is considered important on the basis of the above mentioned. This programme is undertaken in co-operation with the Research Institute for Aquaculture, Fisheries and Irrigation, University of Debrecen, Faculty of Agricultural Economics and Rural Development and the Association of Hungarian Fish Farmers and Product Council.
The European Commission proposed the formulation of the European Fisheries and Aquaculture Fund (EFAF) for the period 2007-20013, which will replace the Financial Instruments for Fisheries Guidance (FIFG), but it also consists of several new elements and will be working differently, too. According to the proposal the budget for the Fund will be nearly 5 billion EUR (4963 million EUR). The development level of aquaculture and fisheries and the social and economic significance in the given member state will be considered when distributing the Fund between the Member States.
According to the plan the Fund is organised along five priority axes, of which the most important for the Hungarian fisheries sector is No. II: Aquaculture and the processing and marketing of aquaculture and fisheries products. The main measure areas are the followings:
1. investment support for aquaculture;
2. support for aquatic-environmental schemes;
3. environmental- and animal health issues;
4. investments in processing and marketing
In case of accessing support under measure area No. 2 farms are obliged to meet the requirements of the scheme beyond the “good management practice” for 5 years, which is to be supervised by the approved body of the Member State. For this reason our work is considered to be substantial.
Approval of the application of “Good Pond Culture Practice” is based on two elements: first the prevailing environmental and nature conservation regulations, as well as the list of controllable conditions in the new agri-environmental agreements are to be met. “Good Pond Culture Practice” are to be conducted on the whole farm area. Its main elements are:
- nutrition management,
- feeding,
- pond maintenance,
- stocking,
- harvesting,
- animal welfare (storage and over-wintering). -
Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:83We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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Experiments on the Nutrient Removal and Retention of an Integrated Pond System
18-23Views:183A combined intensive-extensive fishpond system developed for the purification and re-use of intensive fishpond effluent water was studied during a three-year experimental period. The investigated pond system consists of five small-size intensive culture ponds of 1 ha total water surface area with 1.5 m water depth and a 20 ha extensive culture pond with 1.0 m average water depth. The water was recirculated between the intensive and extensive ponds with around 60 days retention time in the extensive treatment pond.
Carbon, nitrogen and phosphorus budget and water purifying capacity were described and evaluated by means of regular measurements of nutrient concentrations in the water and sediment. During the three-year test period, 81.5% of organic carbon, 54.7% of nitrogen and 72.2% of phosphorus were retained by the system as a percentage of the total input of each nutrient. A significant amount of the total nitrogen input was removed by the harvested fish, which was much higher than in traditional fishponds or intensive fish culture systems. The efficiency of nutrient removal is clearly indicated by the 27.3% nitrogen assimilation.
Only a small percentage of the total nutrient input was discharged into the environment during fish harvest, which was 9.0% for organic carbon, 13.2% for nitrogen and 12.1% for phosphorus. The combination of intensive and extensive fishponds with water recirculation resulted in significant reduction of nutrient discharge into the surrounding aquatic environment, primarily due to the high nutrient processing and retention capacity of the extensive fishpond ecosystem. -
Dilemmas of interpreting folk culture in the cross-border regions of Hajdú-Bihar and Bihor counties
73-78Views:113Identity connected to the dwelling-place has been, and still is, shaped by many (regional, natural, cultural, administrative, and historical) factors. The Euro region of Hajdú-Bihar–Bihor can be considered as a sort of transitional region, which has a strong impact on the cultural identity of both individuals and small communities. The identity construction of local inhabitants, however, does not necessarily follow the different geographical, ethnographical and historical borders of subregions. As a result, several transitional zones exist, which are now divided by a national border, too. Consequently, although many research theories and aspects have been conceived, no uniform method of approach has been created, which would define exactly the classification of folk culture in terms of small, medium and large region. The present short study examines problems posed by this question.
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Connections between the folk dance culture and regionality in the area of Derecske and Mikepércs
49-54Views:114The culture of folk dance has been prominently cultivated in Hungary nowadays, however some regions are yet to be discovered by ethnographers. Stopping this gap by this time is almost impossible due to the small number of informants. Nevertheless, memories of ethographers’ collections, motion pictures, photographs, notes and the choregraphies made from them endure, on the basis of which traditions can be reconstructed, that were so typical of some townships at one time and have been forgotten by now. What can a recreated tradition mean to a community, that sometime was left to waste by them? This is the problem the article is dealing with through the history of Derecske and Mikepércs, two townships in Hajdú-Bihar county, revealing the regional relationship system as well as late and recent potentials of the area.
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Increase of Wheat (Triticum aestivum L.) Resistance to Leaf Rust (Puccinia tritici) via Gene Transformation
127-129Views:69Leaf rust is one of the most significant fungal disease of wheat not only in Hungary but also in other parts of the world. For improving leaf rust resistance of winter wheat variety (Hajdúság, 2003) produced by conventional breeding methods, verified by results of variety tests, showing outstanding results in the aspect of the most important economic values, integration of tissue culture technics, genetic engineering and traditional
methods may provide facilities. Building the gene(s) responsible for resistance into the determined genome can improve the resistance in a way that changes other features of the plant slightly or not at all. In the course of genetical transformation of the variety Hajdúság we applied one of the wheat’s own effecient green-tissue specific insurer genetical regulator, the promoter of ribulose carboxylase 1-5 bisphosphate (RuBisCo) ‘s small
subunit to control the expression of the gene cmg1. -
Comparative Study on Different Water Bodies and their Sediments
17-21Views:68Hungary is rich in natural water resources, therefore investigation of these biotopes is an important task. The Szarvas- Kákafok deadarm is the largest horse-shoe lake of the Tiszántúl, its lenght is 28 km. It has an important role in recreation and agricultural utilization. The quality of the deadarm is influenced by the river Körös, but also by communal and agricultural pollution. Our goals were to estimate the intensity of the
sedimentation processes, and the water- and the sediment quality as well. Also, we examined an artificial wetland system, constructed by the Fish Culture and Irrigation Research Institute, Szarvas.
The calculated sedimentation was 0.4-0.5 cm year-1, which indicated an intensive eutrophication process. The constructed wetland system was able to decrease the organic load of the intensive fish culture company. On the basis of our TOC measurements, the organic matter content of the effluent water remained whitin the water quality limits.
The changes of the material cycling could be more intense in the water body, then in the sediment. The oxido-reduction potential of the sediment could indicate the ecological state of the shallow lakes, therefore it could be an easily measurable indicator in the water classification. -
Values of Hungarian pigeon breeding
9-14Views:153Pigeon breeding has a history dating back several centuries in Hungary. It has internationally renowned and accepted results. Our aim was to estimate the range of values related to Hungarian pigeon breeding and draw attention to the importance of their conservation. We studied the biological values of native breeds and the appearance of pigeon breeding within the Hungarian culture. These include the use of the symbol, the relating beliefs and customs, language, gastronomy, architecture and object values, as well as the community-building effect. Based on our results we can say that this topic is worthy of further research.
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Study of the Interaction Between Common Carp (Cyprinus carpio L.) and Silver Crucian Carp (Carassius auratus gibelio B.)
16-19Views:81During the last few years, the spread of the silver crucian carp caused significant problems in pond fish culture. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the breeding season, weekly test fishings were made to examine the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower productivity and growth rates in common carp. In the control pond (with no silver crucian carp), the average weight of the common carp was over 20 g and the yield/hectar was over 700 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 4.5 g and the yield/hectar was only 123 kg.
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Comparision of growth of mature all-female and mixed-sex Common carp (Cyprinus carpio L.) stocks in RAS
65-68Views:422The common carp (Cyprinus carpio L.) is the most important fish species in Hungary, it is more than 70% of the total Hungarian fish production. The common carp production is important not only just in Hungary but in Middle-East Europe, as well as Southeast Asia. Majority of the production comes from fishpond culture. If the production sector wants to meet the increasing customer demands, there is need to intensify research on the intensive fish production opportunities for example all-female common carp technologies. The all-female technology is one of a genom-manipulation technology. Its production showed better growth rate than mixed-sex population in pond culture. Our experiment combined the recirculation aquaculture system (RAS) and the all-female common carp stocks intensification technologies. The reason for the experiment, is that there are no result about the growth of all-female common carp growth in RAS.
The experiment used the „Tatai grey scale type” common carp stocks. We propagated two all-female stocks (T2 and T3) and a control group (TK). Due to technological characteristics of RAS, the water quality parameters were the same for all treatments and corresponded to the technological tolerance of common carp.
The experimental period was from July 10, 2019 – November 20, 2019. Result of growth performance showed that the growth of mixed-sex stock was significantly higher than all-female stocks; (Control=3692.0±590.5g, T2=3438.8±415.4g, T3=3294.1±659.1g). Feed conversation ratio (Control=1.3±0.1 T2=1.5±0.2 T3=1.6±0.5) and SGR% (Control=0.8±0.0 T2=0.7±0.1 T3=0.7±0.1) were similar.
By the results it can be said the all-female common carp technology has neither advantages nor disadvantages compared to the mixed-sex stock. The all-female technology can be beneficial if the consumers need female common carps. It is worth continuing the experiment and examine how the stocks will perform above 3kg body weight.
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The effects of education, culture, research and development in Hajdú-Bihar county
87-90Views:161The most important instrument of social advancement and economic development is the education system. The educational qualification of the population of Hajdú-Bihar county is continuously improving, however the census data of the year 2001 show that the educational qualification of the population of the county did not reach the national average. The educational qualification of the county’s population is higher at the county seat and in other cities and lower in small municipalities. The county’s ratio is strongly influenced by the data of the county seat Debrecen and that of Hajdúszoboszló sub region.
The unfavorable educational level of the region is related to the migration tendencies and the brain-draining trends of the past decades, as a consequence of which the population of the less developed eastern regions has moved either to the capital, or to the county seats, or larger cities of the region. The young are increasingly affected by the process.
The number of kindergartens in the county corresponds to the national average, therefore taking into consideration the regional demographic characteristics the aim should be the equalization of regional disproportions. The number of students per 1000 resident within the Northern Great Plain Region and in the county significantly exceeds the national average. The secondary school network of the county consists of 38 secondary grammar- and 51 secondary vocational schools.
The secondary schools of Debrecen have considerable impact on the county’s secondary education. The vocational training network of Hajdú-Bihar county is a large, in its conditions highly differentiated institutional network in which all types of secondary vocational institutions are present with secondary technical-, vocational-, accredited higher vocational-, specialized or preparatory vocational-, and special skill enhancement programmes. The majority of the secondary institutions have regional, rarely national enrollment.
Students who have the intention to continue their studies in higher education within the county can study in Debrecen or Hajdúböszörmény.
The integrated University of Debrecen has the highest student number within the country and it offers the widest range of academic programmes in Hungarian higher education. The University of Debrecen with the wide range of graduate and postgraduate educational programmes, unified academic qualification system, English language programmes is a European standard scientific centre of the Trans-Tisza Region. The county’s higher education takes exceeding share beyond its weight in the education of Natural Sciences (Biology, Physics, Chemistry, Earth Sciences, Mathematics), Health- and Social Sciences, Agricultural- and Environmental Sciences and Information Technology, which positively influences its educational structure. The county’s share in the education of Humanities (Hungarian Language, Foreign Languages, History, Archeology, Philosophy) is also significant.
6.3% of the country’s scientific researchers work in the county. In 2010 7.0% of the country’s R+D expenditure was spent here. 3/4 of the region’s research capacity is in Hajdú-Bihar county employed either by the University of Debrecen or by the research teams of the Hungarian Academy of Sciences.
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Examination for employee’s satisfaction to develop the organisational culture
106-109Views:74This examination for employee’s satisfaction analyzes relationships which influence the general feelings of workers and which are revealing in horizontal and vertical. On the whole we can observe that the workers find their horizontal relationships good. In the co-worker’s relationship the appreciation and the confidence appear. Inside departments and between departments the good relationship is typical which is indispensable for the effective work. It is interesting that more than 40% of employees find their chief as „ideal superior”. The quality of vertical relation is determined by human relationships. This result is interesting because the management style is not participative in this organisation.
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Identification and biological examination of some inland Monilinia species
27-29Views:113The aim of this study was to identify and biologically analyse some Monilinia species from Hungary. 146 M. fructigena and 28 M. laxa species out 174 infectious fruit from all over the country were used for the study. For further study 10 isolates were used and apple fruit was inoculated with them according to Koch postulate. 1–2 mm ochre exogen stromas were observbed on infectious plant parts and growing signs on culture of all isolates were identical to M. fructigena. To affirm classical identification, isolates with molecular biological method were also prepared using PCR reaction. Control isolates of M. laxa, M. fructigena and M. fructicola were used. The size of PCR product showed that all isolates had a 415 bp band which was typical of M. fructigena. Results support the previous observation that M. fructigena and M. laxa species occure all over Hungary.
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Phylogenetic studies of soybean pathogen Phoma species by Bayesian analysis
53-61Views:119We carried out phylogenetic study analyzing sequences of genetic markers in the taxonomy of Phoma and Phoma-like fungi. Different species of Phoma and Phoma-like fungi occurring on soybean (Phoma pinodella, Phoma sojicola, Phyllosticta sojicola, Phoma exigua var. exigua) are difficult to identy because of their high morphological and symptomatic similarities.
Twenty-two isolates of nine different Phoma species were obtained from reference culture collections. Seven of them were isolated from soybean, the others were collected from different hosts.
The Phoma isolates were firstly characterised by morphologically, and then we employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1), ITS region, as well as β-tubulin partial sequences as potential genetic markers to infer
phylogenetic relationships among different Phoma species..Finally, their ITS and tef1 sequences were sequenced and analysed by Bayesian approaches.
According to phylogenetic trees inferred by Bayesian analysis of tef1, ITS and β-tubulin sequences, different Phoma species can be separated proving that these phylogenetic markers are well suited for phylogenetic studies of Phoma species. However, the phylogenetic tree does not support the traditional Phoma sections based on morphological characterization.
Bayesian analyses of the three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The molecular data provide evidence for reclassification of formerly mentioned soybean pathogens. -
Experiments for Isolating and Culturing Soil-borne Mycobacteria (Contemporary Publication)
107-110Views:59On grounds of the several thousand tests performed in the field of this topic, the following conclusions may be arrived at:
1. The informations available and the experimental data on soil mycobacteria are very incomplete.
2. Of the 77 strains isolated from similar soil types so far, and adaptable for pure basic culture, 47 strains are confusingly similar, from morphological aspects, to the mycobacteria isolated from clinical material.
3. The apparently homogeneous cultures isolated from the soil are generally co-infected and, therefore, the morphological, biochemical, and other physiological characteristics of the isolated strains can be studied only on base cultures after purification.
4. For the isolation of the soil mycobacteria experiments qualified hitherto as most suitable processes the 4 or 1 per cent NaOH neutralized with H2SO4, and the 1 per cent NaOH or 1 per cent Na3PO4 treatments, on Gottsacker agar medium with plate or top pouring, at a temperature of 29 to 37 C°, in a soil suspension sequence of 1:500 to 1:5000 final dilution.
5. The Ziehl-Nielsen staining of the isolated mycobacteria composed to sub-cultures is best performed by heating with an infra red radiator from above, instead of the gas flame used so far to heat from below.
The repetition of the biochemical test of the hitherto isolated 77 purified strains is under progress, and will be reported on in our next scientific publication. -
Challenges and responses for the convergence of people living in deep poverty in Hungary
51-54Views:126The accompanying phenomenon of the ongoing economic crisisis the deepening and fixation of awidely reaching social crisis. The concept o fdeep poverty has become generally known in recent years and has now become inevitable the convergence of the most disadvantaged at EU, governmental,regional and local levels. Besides government and othe rprograms a lot of research and studies serve reintegration. Among convergence tools education and training play prominent roles, which besides the acquisition o fpractical knowledge, place special emphasis on the development of a convergence helping environment and culture, the creation of a "convergenceculture".
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Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:86Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Tending operation model for red oak stands
133-136Views:158Red oak (Quercus rubra L.) is our most important exotic oak species, that possesses growing culture with almost one hundred years in our country. Plantation character of red oak growing predominates principally by applied regeneration method (mostly artificial), regular planting space, applying defined method and planting space regulation system. Forest tending model for red oak, that considered stopgap in the native literature is intended to serving the latter. Especially important equation in the viewpoint of stand growing, that describes relationship of stem number (N, in hectare) and diameter at breast height (D1.3 in cm) is: N=e9.80220-1.12607lnD1.3.
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Phytopatological properties of symbiotic Rhizoctonia solani strains associated to orchids
65-71Views:143The mycobiota of the Orchidarium of ELTE Botanical Garden (Budapest) has been studied applying aerobiological methods and isolating of tissue samples taken from 92 individuals of sixty orchid species. Among isolated basidiomycetaceous fungi 13 strains of Rhizoctonia solani were surviving in axenic culture. These symbiotic R. solani strains proved to be pathogenic on 24 cultivated plant species at varying degree. The symptoms of disease caused by R. solani strains isolated from orchids did not differ from that caused by reference strains. Three groups of strains could be separated regardless of their source or aggressivity. The host plants clustered into two groups, and their taxonomic position had no role in this respect. In general, we can assume that orchid associated Rhizoctonia strains are potential plant pathogens, and removed or withdrawn orchid stools should be treated as hazardous waste.
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:90Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
The Biology, Ecology and Medicine Faculty of Dnipropetrovsk National University after Oles’ Gonchar
15-17Views:67The historical rewiev of Biology, Ecology and Medicine Faculty is presented. The Faculty of Biology, Ecology and Medicine has 7 Departments, Aquarium complex, Zoological Museum, Vivarium and Herbarium. It works in cooperation with the Research Institute of Biology, Botanical Garden, O. L. Bel’gard International Biosphere Station, and Biological Station of DNU and forms the regional Centre of Science, Education and Culture in the field of Biology, Ecology and Nature Conservation in Central Ukraine. The Faculty proposes courses in the following specialities: Biology, Zoology, Botany, Microbiology and Virology, Biochemistry, Physiology, Ecology, Environmental Protection and Balanced Nature Management. All of them have the highest IV level of accreditation. Students get a pedagogical education.