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Examination of the presence and effect of udder edema in Holstein-Friesian cattle
125-130Views:180Mastitis is the most common disease of dairy cows and the most expensive in terms of treatment. For this reason, great emphasis must be placed on its prevention. Based on literature sources, it can be said that there is a positive correlation between the prevalence of udder edema and the occurrence of mastitis. Our studies were carried out in a dairy farm in Csongrád-Csanád county. So far we have collected data from 64 cows with udder edema. The severity of udder edema was evaluated on a scale of 0–3 points one week before calving and for 3 weeks after calving. We observed that udder edema occurred in 78% of the studied cows at the time of the first calving. After calving, the length and diameter of the four udder teats were examined. Both anterior and posterior teats were shortened in severely edematous cows. When examining the diameter of the teat, we found that, depending on the severity of the edema, the anterior and posterior udder teats were 2.5 and 1.6 and 1.6 and 1.5 mm thicker respectively than those of cows without edema.
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Changing of some parameters of the soil-plant system as an effect of different composts
262-266Views:137Composting is an alternative way for practicing site-specific and environmental friendly plant nutrient supply. Our aim was to study the effect of different composts on plant and soil system.
Pot experiment with acidic sandy soil blended with three composts in five (0%, 5%, 10%, 25% and 50%) proportion was set up. Our experimental plant was perennial ryegrass (Lolium perenne L.). After the harvest of ryegrass we measured the fresh and dry weight of harvested plants, the P-, K-, Mg-contents, and the pH of soils.
The three composts had different effect on dry weight production and on nutrient-supply of soil. We established that one of the compost had significantly larger effect on the dry weight of ryegrass compared to the others. In this study we proved that favourable compost/soil proportion is different in the case of different composts. -
Effects of diets with different fibre content on the performance of rabbit does and on parasitological infection
41-44Views:129The effect of two diets with different fibre contents was examined on rabbit does’ performance and parasitological infection. Diet P2 had 1% higher crude fibre, ADF and starch contents than that of diet P1. The performance of 12–12 does and litters per group were examined during two consecutive inseminations. In both dietary groups, according to a 49-day reproduction rhythm, the does were artificially inseminated 18 days after kindling. Most production traits were not affected by the diets (kindling rate, weight of does and their kits, feed intake, mortality; P>0.05). Significant differences were only found in litter sizes as the number of kits was lower in P1 diet fed group at all examined days of lactation. The significant “Diet x Reproduction cycle” interaction showed that the litter sizes of dietary groups did not differ at the first examined insemination, however at the subsequent reproduction cycle 6–8% lower litter sizes were counted in P1 fed does compared to group P2 (litter size at 4 day: 9.00 vs 9.58–9.92, P = 0.004; litter size at 11 day: 8.83 vs 9.58–9.92, P = 0.037 for diet P1 at the 2nd reproductive cycle compared to all the other cases, respectively). The two diets with different compositions did not affect most of the production results of the rabbit does and their kits, but the lower litter size of does consuming P1 diet at the second examined lactation suggests the adverse effect of P1 diet’s long-term use. From the collected manure samples not any parasites were detected which presents a very favourable picture of farm from the point of view of hygiene and animal health.
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The effect of climatic change on the rheological properties of winter wheat doughs
96-100Views:123In present paper we have examined the effect of climatic change on the extensigraph characteristics of wheat-flours. The baking quality
of winter wheat is largely determined by cultivar, but it can be influenced by weather conditions during growing period. Flours were from 5
cultivars grown at one location in three cropping years. We have found that the extensigraph properties of dough are affected by the weather
conditions, nevertheless, different cultivars distinctly react to the increase of temperature and decrease of precipitation. In generally, the
higher average day temperature and lower precipitation level is favourable to produce winter wheat flour with better quality -
Comparison of Voluntary Feed Intake and Venom Production of Wild and Laboratory Bred Sand Vipers
27-29Views:87The study was performed on vipers of the Vipera ammodytes ammodytes species and aimed to establish the differences in voluntary feed intake and venom production between a group of wild, recently captured vipers and a group of born and bred captive vipers. In addition, the influence of sex on both parameters was established. The research brought evidence of important differences concerning voluntary food ingestion and venom production between the two groups of animals. However, sex appeared not to significantly influence these parameters, both in wild, recently captured vipers and in born and bred captive vipers. Wild animals rapidly accommodated to the microclimate conditions in captivity and readily accepted food.
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Comparative study of special honey products and herbhoneys
117-120Views:390Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
The effect of season on the microbiological status of raw milk
95-99Views:182Many factors can influence the microbiological quality of raw cow’s milk. In this study, our aim was to determine whether there was any difference between the microbiological statuses of milk produced in different seasons. Samples were collected and analysed from five dairy farms in Hajdú-Bihar County, from February to November in 2019. During our studies, total plate count (TPC), coliform count and Staphylococcus aureus count of raw cow’s milk samples were determined.
There was no significant difference (P>0.05) between the mean TPC values detected in the milk collected in winter and autumn, but that values were significantly (P<0.05) lower than in the milk samples collected in spring and summer. Similarly to the TPC, in the case of coliform bacteria the lowest mean colony count was detected in the samples collected in winter. The difference was significant (P<0.05), compared to the values observed in the samples collected in summer. S. aureus was detected in bulk milk of only two farms in excess of 1.0 log10 cfu/ml. Also in case of S. aureus, there was a significant difference (P<0.05) between the values observed in the samples collected in winter and in summer. Samples from spring and summer contained the highest amount of S. aureus.
Based on the results of our studies, in the case of almost all farms the mean TPC, coliform and S. aureus counts were lower in the samples collected in winter, than in the samples collected in summer. The fact that the samples collected in winter contained the lowest amount of colonies could be attributed to the inhibition of growth of mesophilic microorganisms below 8 °C. Furthermore, the fact that we observed the highest colony counts in samples collected in summer, can be related to the heat stress of cows during the summer due to unfavorable weather conditions (high temperature and humidity).
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The Effects of Corn Cobs in Feed
51-54Views:117This study is part of a larger research work that aims to establish the usefulness of corn cobs, a low cost dietary resource, in the growth of ruminants. Corn cobs are found in large amounts in our country (8.2 mil. tons/year). Increasing the quantity of corn cobs to 50% of the diet in lambs resulted in a decrease by 14.57% in the concentrate intake that is needed to obtain one-kg weight increase. In addition, the diet costs were reduced by 16.33% (Mierliţă, 1999). Increasing the quantity of corn cobs to 20-50% of the diet also resulted in multiplication of bacteria from genus Ruminococcus, that are known to represent about 70-80% of the bacteria population in the rumen. In addition, an increased multiplication rate of large protozoas (i.e. Epidinium, Isotrichia, Diplodinium etc) was observed. This explains the high conversion rate of piruvic acid, a carbohydrate fermentation product, into acetic acid, whereas conversion of piruvic acid into propionic acid decreases. In addition, feed intake and the quantity of digested and absorbed fibers increased by 8.46% and 35.09%, respectively. Thus, a reduction in dietary concentrates needed as nutrient supplies was achieved.
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Testing the antimicrobial activity of essential oils
71-74Views:151The vapor phase of some essential oils proved to have antimicrobial activity. Utilization of the vapor phase of Eos is presently understood as one of the possible alternatives to synthetic food preservatives which could be used in the future. However, testing the vapor phase of EOs against microorganisms causing food-borne diseases (e.g. Salmonella enteritidis or Staphylococcus aureus) or food spoilage is relatively new. Consequently, due to the large number of known EOs, research on their antimicrobial activity is still largely in the phase of in vitro rather than in vivo testing. Moreover, no standard and reliable method for fast screening of a wide range of samples exists. Thus, the aim of this study is to show results concerning tests of the antimicrobial activity of EOs against S. enteritidis or S. aureus, which were conducted by two modifications of the disc volatilization method we developed. The lately developed method has the potential to become widely used for fast screening of EO antimicrobial activity in the vapor phase.
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The effect of breed and stage of lactation on the microbiological status of raw milk
37-45Views:340The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.
There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.
Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.
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Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA
29-32Views:253Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.
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Routine microscopy examination of faecal samples as a tool for detection of common gastrointestinal parasites: a preliminary report from two Hungarian farms
63-66Views:157Gastrointestinal parasitism in ruminant animals is a cause of major economic loss incurred by the livestock industry. Regardless of the frequency of the adopted therapeutic and prophylactic deworming strategies, the parasitic burden in a farm should be assessed regularly. One of the most widely used techniques to do so is the microscopic faecal egg examination and faecal egg counting method. Despite the technique being almost a century old from its first adoption, the principle behind the newer techniques of faecal egg examination is the same. This technique is still being used in routine farm screening and monitoring gastrointestinal parasitic load and faecal egg count reduction testing to assess the anthelmintic efficacy of the drugs used. Thus, the tool remains a choice for preliminary screening for important parasites and the subsequent deworming strategy. Our study here was part of a larger survey on the treatment efficiency as well as a broad epidemiological study of the trichostrongyle parasites in Hungary. We present a preliminary report on the detection of common gastrointestinal parasites from two farms in Hungary, including a species-specific confirmatory microscopy for Haemonchus contortus eggs.
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Effects of combined nutrient supply treatments on some physiological parameters of autumn wheat
241-251Views:153The Fleischmann Rudolf Research Institute in Kompolt is not only famous for plant breeding but the institute also surveys the effects of different nutrient supply methods since 1918. In 2017, we joined this research supported by EFOP 3.6.1 project. Our aim was to investigate photochemical processes – which is one of the most determinant in case of yield – of crops by in vivo field measurements. We measured the chlorophyll content of leaves using Minolta SPAD 502. We used miniPAM fluorometer to determine actual photochemical efficiency and non-photochemical quenching of PSII during natural light conditions and also to evaluate the pigment (chlorophylls and carotenoids) and water content of leaves we applied field spectrophotometer (ASD FieldSpecPro 3). We utilized these methods by various treatments (1. treatment with soil bacteria + head and base fertilizer; 2. treated by only head fertilizer; 3. treated by only base-fertilizer) in field experiment of autumn wheat (4.1–2.43–1.19 ha) in June, 2017. The difference between treatments was clearly detectable. In the case of the first treatment, physiological processes were more intense and the ripening occurred earlier. The obtained yield was the highest in the case of the area treated by soil bacterial. Based on the results, the first treatment can be recommended in practice.
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Biological control of sweet chestnut on Pécsbánya, Hungary
77-81Views:252The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
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Preliminary studies to evaluate the use of spectral data in monitoring of apple orchard parameters
37-41Views:134The introduction/application of precision agricultural technologies has more important role in various fruit growing sectors among others apple growing. Remote sensing methods can detect electromagnetic waves where the green colour of the leaf is responsible for the chlorophyll content. The absorption of chlorophyll is in the wavelength range of 450–670 nm. Samples of apple tree leaves were taken on a weekly basis from the apple orchard at Horticultural Unit of Pallag on University of Debrecen in 2019 summer. Our studies were performed on 2 cultivars (Early Gold, Golden Reinders) and the samples were processed using 2 methodologies: a non-destructive spectral method and spectrophotometric method chlorophyll and carotenoid contents were calculated, which were created into some groups and compared with the spectral values. When the plant begins to lose strong green colour and turns yellow spectral measurements show that chlorophyll content decreases as the proportion of chlorophyll-carotenoid in the plant changes. In case of grouping into intervals, it can be observed that as the chlorophyll content increases the reflectance value decreases continuously due to the strong absorption. Based on the results, close relationship between the pigments can be detected.
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The effects of agrotechnological factors on winter wheat yield in humid cropyear
162-167Views:129The effects of crop rotation, nutrien supply and crop protection technologies, as well as the appearance of the main ear- and leafdiseases
(powdery mildew, helminthosporium leaf spot, leaf rust, fusarium) were studied on the crop yields of winter wheat variety MV
Pálma during the 2009/2010 crop year. The experiments were conducted in triculture (pea – wheat – corn) and biculture (wheat – corn), at
five nutrition levels, with the use of three crop protection technologies (extensive, conventional and intensive) at the Látókép Research Site of
the University of Debrecen, Centre of Agricultural Sciences. Our results proved that the appearance of leaf- and ear-diseases were
significant in the wheat cultures during the 2009/2010 crop year, because of the rainy, warmer than usual weather, the lodging, and the huge
vegetative mass developed. The most severe infections by the four examined diseases after pea and corn pre-crops were observed at
extensive crop protection levels, when fertilizers were used at the highest dose.
Following corn pre-crop, in the case of all the three crop protection technologies the maximum rate of wheat yield results were achieved
at N150+PK level. The highest yield was reached at intensive crop protection level (6079 kg ha-1). In triculture, in case of all the three crop
protection technologies the maximum yields were achieved at N50+PK level; in extensive technology 5041 kg·ha-1 yield, in conventional
technology 6190 kg ha-1 yield was realised, while in the intensive technological model the yield was 7228 kg ha-1.
The relationship between yield and fertilizer amounts, the rate of pathogen contaminations, crop protection technologies and pre-crops
was defined with correlation analysis in case of different crop rotations during the 2009/2010 crop year. Based on the results of the
experiment, we found that in stands after corn pre-crop strong positive correlation was established between the crop protection level and the
crop yield (0.543), the nutrient levels and the emergence of the four examined pathogens, and between the nutrient levels and the yield
(0.639). Extremly strong positive correlation was observed between crop protection and yield (0.843) in triculture. Strong positive
correlation was detected between the nutrient levels and the presence of the four examined pathogens, as well as between nutrient and
lodging (0.688). Strong negative correlation was between the crop protection level and the four examined diseases both in biculture and
triculture. -
Soil Biological Activity within Integrated and Ecological Management of Soil
47-52Views:88The effects of the integrated (IS) and ecological (ES) management of soil on chosen parameters of soil biological activity were investigated in the period 1999-2000. The following characteristics were determined: biomass of microorganisms (Cmic), dehydrogenase activity (DHA), an amount of potentially mineralizable nitrogen (Nbiol), and nitrification intensity. Soil samples were collected from a stationary field experiment established in 1990 on gley brown soil at the Experimental Station of Slovak Agricultural University, Nitra. For each field with a different crop rotations two fertilization treatments were selected: (a) no fertilization and (b) use of manure for silage maize and, within IS, also mineral fertilizers. There was a statistically significant difference at α = 0.05 in the amount of biologically released nitrogen (Nbiol) between both systems and in the nitrification intensity in favour of ES. A higher amount of microbial biomass (Cmic) was noted for ES but without statistical significance. Cultivated crops and the timing of soil sampling were found to have the greatest effect on all the parameters observed in individual experimental years and within the two systems of soil management.
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Experience at Russian State Agrarian University – Moscow Timiryazev Agricultural Academy on introduction and integration of precision agriculture technology
73-76Views:117Traditional and precision agriculture technologies are compared on the basis of the field experiment. Problems of soil and crop survey and mapping are discussed.
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Biomass production estimation of processing tomato using AquaCrop under different irrigation treatments
131-136Views:215The wiser usage of irrigation water is inevitable in the future. Irrigation has very high input cost; therefore, farmers must carry out irrigation with care. Also, the effect of irrigation on crops has a big role in decision making. Modeling provides a possibility to evaluate this effect. AquaCrop, as a crop production simulation model has great potential in this field. The accuracy of tomato biomass yield prediction of the model was tested in this research. For collecting the necessary data, a field experiment was conducted at Szarvas on processing tomato with different water supplies, such as 100% (I100), 75% (I75), 50% (I50) of potential evapotranspiration and a control with basic water supply (C). The relation of the simulation and actual biomass yields was evaluated during the season. Very good correlation was found between the modelled and the actually harvested data. The data for the control and I100 treatments showed higher correlation than the I75 and I50. The relationship for all of the data was moderately strong. Miscalculations occur mostly when the dry biomass yield reaches
7 t ha-1. The accuracy of the model was evaluated with the use of mean absolute error (MAE) and root mean squared error (RMSE) values. The least error was found in the C treatment, which means 0.34 MAE and 0.45 t ha-1 RMSE. The simulation resulted in higher errors in the I75 and I50 treatments. -
Effects of fermented and supplemented chicken manure on the nutrient management aspects of an apple orchard
117-123Views:145AIt is a huge challenge for farmers worldwide to successfully increase the organic matter content of their soils and improve their water balance at the same time. Therefore, the main aim of the study is to develop and test organic-based nutrient composite materials that can be successfully used by farmers to increase soil organic matter content, improve water management parameters and implement water-efficient technologies. The study was performed in the orchard of the Institute of Horticultural Science of the University of Debrecen in Hungary (Debrecen-Pallag). The experiment was set up in a ten-year-old apple (Malus domestica ‘Pinova’) orchard. In the trial, fermented poultry manure and superabsorbent polymers (SAP) were used at different doses to study their effects on soil properties and fruit quality. Applied composite materials increased the nitrate and organic nitrogen content of the soil. Treatments did not affect the sugar content of the fruits but significantly and positively affected the individual fruit weight and the titratable acidity of the fruits.
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Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
23-28Views:382A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.
On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.
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Rye plant parameters in the Westsik crop rotation experiment
39-45Views:128Our research work was carried out in the Westsik crop rotation field experiment in 2018. The main research purpose was to analyse the effect of the different organic and chemical fertilizers on parameters of rye. Our results revealed some differences between the different fertilization methods. One spike weight, grain weight of one spike, rye plant height, rye plant weight per m2 and 1000 seed weight in crop rotations VII, XV and VIII were different from the data of all crop rotations. This finding can be explained by the fact that crop rotations VII and XV were non-fertilized, only 23.3 t ha-1 straw manure (VII) or green lupine manure was applied as a second crop (XV). In addition, crop rotation VIII consists of four parts where we apply chemical fertilization with green lupine manure as a main and second crop. There is a positive close correlation between rye plant height and other studied characters (rye plant weight per m2, spike length, weight of one spike, grain weight per spike, spike weight per m2, grain weight per m2 and 1000 seed weight).
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Wheat cleaning and milling technologies to reduce DON toxin contamination
89-95Views:266Mycotoxicosis caused by Fusarium fungi holds a huge risk considering economic and food safety issues worldwide. By applying milling technologies, we attempted to reduce the concentrates of DON toxin, as it is the most often found toxin in wheat.
The processes of sieving, aspiration and combination had been used on wheat with high DON toxin concentration. As a next step, grains were sorted using a horizontal cylinder separator, assorted by an optical and a gravity separator, and finally, the products were scoured and ground. The contamination level of the wheat and flour samples were defined by the HPLC-MS method.
Regarding the results, it can be stated that toxin concentration was most effectively reduced by optical separation and scouring, and by applying these milling techniques, food safety can be increased significantly.
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Determination of element contents of commercial and homemade jams
71-75Views:285Various products could be prepared by using fruits as raw materials. In Hungary, the most popular fruit products are spirit, jams, preserves, juices and dried fruits. Jams are made from different fruits, with different fruit content, technology and additives. They can be used for different products or they can be consumed by themselves. These products and their related production processes have great traditions in Hungary. In addition, they are widely used.The aim of our study was to determine the element content of jams and to compare these samples based on their micro- and macroelement content.In this study, 26 fruit jams with different botanical origins were examined. 10 samples were commercial, and 16 samples were homemade products. The determination of micro- and macroelements was carried out by using Inductively Coupled Plasma Mass Spectrometer and Inductively Coupled Plasma Optical Emission Spectrometer. Different element contents were measured [B (249.7), Ca (422.6), Fe (259.9), K (769.8), Mg (280.2), Na (589.5), P (185.9), S (182.0), Al (27), Mn (55), Cu (65), Zn (66), Sr (88), Ba (137)]. Based on element contents, the jams showed statistical differences. -
Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:181Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.