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Habitat suitability modeling of a Subterranean mammal in Iran: case study of the Western Mole-Vole (Ellobius lutescens)
113-120Views:541Rodentia is the largest order of mammals in Iran, distributed in almost all types of habitats. Subterranean mammals, such as the Western Mole-Vole, have an important role as ecosystem engineers by creating underground burrows and tunnels and aerating, propagating seeds, and building shelter for other small mammals and animals. They are also a keystone part of the food source for other prey vertebrates. However, many areas are considered pests, and their habitats are fragmented due to human activities, which causes a reduction in their habitats and colonization. Habitat Modeling tools help us to predict a suitable niche for conservation planning. The purpose of this study is to predict habitat suitability for Western Mole-Vole in Iran by using the MaxEnt method to determine which part of Iran is a desirable habitat for this species. The result indicated that the northwest of Iran, especially the southern slopes of the Alborz Mountains and the Eastern slopes of the Zagros Mountains, provides suitable habitats for the western Mole-Vole. The environmental variables that are more influenced by the distribution of this species are elevation of 1800 to 2500 meters, human activities such as roads, and rainfed farms. Approximately 7% of Iran is a suitable habitat for the Western Mole-Vole.
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Effects of diets with different fibre content on the performance of rabbit does and on parasitological infection
41-44Views:300The effect of two diets with different fibre contents was examined on rabbit does’ performance and parasitological infection. Diet P2 had 1% higher crude fibre, ADF and starch contents than that of diet P1. The performance of 12–12 does and litters per group were examined during two consecutive inseminations. In both dietary groups, according to a 49-day reproduction rhythm, the does were artificially inseminated 18 days after kindling. Most production traits were not affected by the diets (kindling rate, weight of does and their kits, feed intake, mortality; P>0.05). Significant differences were only found in litter sizes as the number of kits was lower in P1 diet fed group at all examined days of lactation. The significant “Diet x Reproduction cycle” interaction showed that the litter sizes of dietary groups did not differ at the first examined insemination, however at the subsequent reproduction cycle 6–8% lower litter sizes were counted in P1 fed does compared to group P2 (litter size at 4 day: 9.00 vs 9.58–9.92, P = 0.004; litter size at 11 day: 8.83 vs 9.58–9.92, P = 0.037 for diet P1 at the 2nd reproductive cycle compared to all the other cases, respectively). The two diets with different compositions did not affect most of the production results of the rabbit does and their kits, but the lower litter size of does consuming P1 diet at the second examined lactation suggests the adverse effect of P1 diet’s long-term use. From the collected manure samples not any parasites were detected which presents a very favourable picture of farm from the point of view of hygiene and animal health.
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The effect of climatic change on the rheological properties of winter wheat doughs
96-100Views:340In present paper we have examined the effect of climatic change on the extensigraph characteristics of wheat-flours. The baking quality
of winter wheat is largely determined by cultivar, but it can be influenced by weather conditions during growing period. Flours were from 5
cultivars grown at one location in three cropping years. We have found that the extensigraph properties of dough are affected by the weather
conditions, nevertheless, different cultivars distinctly react to the increase of temperature and decrease of precipitation. In generally, the
higher average day temperature and lower precipitation level is favourable to produce winter wheat flour with better quality -
Comparison of Voluntary Feed Intake and Venom Production of Wild and Laboratory Bred Sand Vipers
27-29Views:173The study was performed on vipers of the Vipera ammodytes ammodytes species and aimed to establish the differences in voluntary feed intake and venom production between a group of wild, recently captured vipers and a group of born and bred captive vipers. In addition, the influence of sex on both parameters was established. The research brought evidence of important differences concerning voluntary food ingestion and venom production between the two groups of animals. However, sex appeared not to significantly influence these parameters, both in wild, recently captured vipers and in born and bred captive vipers. Wild animals rapidly accommodated to the microclimate conditions in captivity and readily accepted food.
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Effect of different N doses on maize yield and quality
97-101Views:409The effect of N doses on the yield and nutritional values of the Sushi (FAO 340) maize hybrid were analysed in three years (2018, 2019, and 2020). The analyses were performed at the Látókép Experimental Station of the University of Debrecen on calcareous chernozem soil, in a striped, small-plot, non-irrigated long-term field experiment. In the experiment, in addition to the non-fertilized treatment (A0), the N-fertilizer doses were applied as basic fertilizer and top dressing. The 60 and 120 kg N ha-1 dose (A60, A120) applied as spring basic fertilizer were followed by two phases of top-dressing in V6 (V690, V6120) and V12 (V12150, V12180) phenophases; the amounts were +30 and +30 kg N ha-1.
Maize yields were affected to varying degrees by crop year. The highest yields in 2018 and 2020 were recorded in the same V6150 treatment, while in 2019 the highest yield was obtained in the A120 treatment.
Increasing the N doses resulted in an increase in the protein content of the maize kernel. Depending on the fertilizer treatments and the crop year effect, the protein content of maize kernels varied between 6.2–10.2 g x 100 g-1. In all three years, the protein content was the lowest in the control treatment (A0) and the highest in the V6150 treatment.
The starch content ranged from 70.7 to 77.9 g x 100 g-1 in the average of the three years. In 2020, it was significantly higher in all nutrient treatments than in the other examined two years. The highest starch content - except for 2020 (A120, 77.9 g x 100 g-1) - was recorded in the A0 treatment (74.2, 72.3 g x 100 g-1).
The oil content of maize kernels varied between the values of 3.8 and 5.2 g x 100 g-1 in the average of three years. In terms of oil content, the results for 2018 and 2019 can be considered the same, while in 2020 it was significantly lower. Fertilizer treatments did not significantly affect the oil content of maize in any of the years.
The fertilizer dose applied in the V12 phenological phase was not effective in terms of yield and nutritional content (protein, starch and oil content).
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Examination and statistical evaluation of physico-chemical parameters of windrow composting
33-38Views:588The treatment and utilization of plant and animal waste and by-products from agriculture is very diverse. Traditional environmental management practices for waste management have been retained through soil conservation and the applied of recycle degradable organic substances in soil. The management of by-products from agriculture (animal husbandry) is important because a closed loop can be created to utilize by-products (manure, feathers) from the production of the main product (eggs, meat, milk) and to form a raw material for a new product. It is important to treat the resulting by-products, especially deep-litter manure, as it has served as a basis for compost-treated manure to develop an organic-based, soil-conditioning product line. Poultry manure by itself is not suitable as a substrate for aerobic decomposition, so it has to be mixed with other substances (zeolite, bentonite, soil), because of its high nutrient capacity, it is an acidifying substance.
The aim of this study was to compost the mixture of poultry manure and hen manure by the addition of zeolite and to monitor the composting process. It was also our aim to statistically determine the effect of the zeolite on parameters describing the composting process.
The windrow composting experiments were set up in the composting area of the University of Debrecen, Institute of Water and Environmental Management. The composting experiment was 62 days long, during which the main parameters describing the composting process were continuously monitored: temperature (°C), moisture content (w/w%), electrical conductivity (mS/cm), organic matter content (w/w%), examination of nitrogen forms (w/w%). In this study, three factors were investigated: temperature, humidity, and pH. For statistical evaluation, R software and RStudio user interface were used. We developed a repeated measurement model, in which the fixed and random effects were determined for our parameters under study, and the resulting relationships were shown on interaction plots.
Based on our results, the temperature of the prisms has become independent of the ambient temperature and the composting stages can be separated in both the control and the zeolite treated prisms. In the repeated measurement model, we proved that treatment, time and treatment: time interaction were significant at both temperature and pH.
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Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:576In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. -
Comparison of the technological background of aquaponic systems
47-52Views:777Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.
The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yield and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NO3¯N were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).
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Effect of Ferilizer on the Yield of Maize (Zea mays L.)
40-46Views:359The effect of fertilization on the yield of maize was examined on chernoem soil with lime deposits at the experimental station at Látókép of the Center for Agricultural Sciences, University of Debrecen. The yields of maize were evaluated using quadratic regression function, in three years – between 2000 and 2002 – in non-irrigated and irrigated treatments. After calculating the regression equations, by derivation of the functions, we have determined the amount of fertilizers needed for maximum yield.
In the non-irrigated treatments, maximum yield and the active substance amount of fertilizer was as it follows: in 2000, yield of 9,133 t/ha with the application of 384 kg/ha mixed active substance, while in 2002 a yield of 6,289 t/ha with the application 236 kg/ha NPK active substance was achieved. In 2001, due to the favourable precipitation, a yield of 9,864 t/ha was achieved with the application of 245 kg/ha fertilizer. In the case of maximum yield, compared to the unfertilized control, the yield increase was 2,5-5 t/ha. The average increase for 1 kg of NPK fertilizer was 13-19 kg.
We also determined the necessary fertilizer dosage for maximum yield in irrigated treatments. In 2000, 10,003 t/ha with a dosage of 423 kg/ha, in 2001, 11,542 t/ha with a dosage of 277 kg/ha and in 2002, 8,596 t/ha of maximum yield could be achieved with a fertilizer treatment of 277 kg/ha in the examined three years. The yield increase, in irrigated treatments, varied between 3,9-5,9 t/ha so it was greater than in the case of non-irrigated experimetal plots. The yield increase for 1 kg fertilizer varied between 12-21 kg. -
Seed treatment with Bacillus bacteria improves maize production: a narrative review
105-111Views:1046Maize (Zea mays L.) is an important crop in relation to its production and consumption. Production of maize is constrained by soil infertility and poor quality seed. Microbial technologies like seed treatment with Bacillus bacteria improves the productivity of maize on infertile soil. However, due to variations in maize growth environments and Bacillus species, this review was conducted to identify the common species of Bacillus species used for seed treatment, and provide an overview of the effect of seed treatment with Bacillus on maize growth and yield. Results show that Bacillus subtilis, Bacillus pumilus and Bacillus amyloliquefaciens were the dominant species used for seed treatment. Bacillus was used as both a biofertiliser and biopesticide. The conspicuous positive effects of Bacillus were in plant height, shoot and root length, and shoot dry matter depending on the species. In terms of grain yield, Bacillus subtilis (8502 kg ha-1), Bacillus amyloliquefaciens (6822 kg ha-1) and Bacillus safensis (5562 kg ha-1) were the bacterial species that had an overall pronounced effect. The highest increase in grain yield was in the interactive effect of Bacillus megaterium + Bacillus licheniformis (18.1%) and sole Bacillus subtilis (15.6%), while Bacillus pumilus reduced grain yield by 4.8%. This shows that the improvement of maize productivity using Bacillus bacteria requires careful selection of the species for seed treatment.
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The use of Common duckweed (Lemna minor) as a biological filter in the rearing of hybrid African catfish (Clarias gariepinus x Heterobranchus longifilis)
107-112Views:613The African catfish (Clarias gariepinus x Heterobranchus longifilis) is one of the most important fish species in Hungarian aquaculture. The larvae and juveniles are reared at high feeding rates, which can reach up to 10% of the biomass. Intensive feeding results in large amounts of effluent water, directly proportional to the intensity of fish production. The aim of this experiment was to investigate the potential of Common duckweed (Lemna minor) as a biological filter and its nutrient removal capacity during intensive fry rearing of the hybrid African catfish. In a recent study, two experimental recirculation systems, identical in water volume and design, were set up, in one of which duckweed was grown under aquaponic conditions, where the concentrations of nitrogen and phosphorus forms were monitored and the production parameters of the fish were determined. The results obtained from the experiment showed that the use of duckweed as a biological filter improves the water quality of the recirculation system. A significant amount of plant biomass was produced, which mainly reduced the nitrate and orthophosphate concentrations in the system water. As a consequence, the survival of hybrid catfish was improved, which also affected biomass growth, but had no effect on other parameters.
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Risk effects of the spread route of mycotoxins
90-95Views:503In Hungary the mycotoxin is a great problem, because there are many natural toxins in wheat and maize. These cereals can be found on
considerable proportion of the country’s sowing area, and they are deterministic food for the population. The direct human and animal
utilization of the contaminated cereals mean a serious risk in the food chain. In Hungary’s climate the soil is contaminated with pathogen
moulds, particularly Fusarium species, which increase by respective temperature and moisture content in cereals. The Fusarium can
decrease the quality of the wheat in different ways: decrease the germination capability and cause visible discoloration and appearance of
mould, reduces the dry material and nutrient content of the grain. From the toxins produced by the Fusarium genus, the trichotecene (T-2,
HT-2, deoxinivalenol, nivalenol, diacetoxyscxirpenol, Fusarenon-X) and the estrogenic zearalenon (F-2) are the most common in Hungary.
The fumonisins (FB1, FB2, FB3) first identified in 1988, relatively newly discovered, are also important. Major proportion of mycotoxins in a
healthy organization is metabolized by the enzyme system of liver and intestinal bacteria. The toxicity is reduced or even leaves off.
However, more toxic and biologically active compounds can be formed. For the reduction of mycotoxin-contamination several possibilities
are available in the case of storage, processing and feeding. -
The current status, challenges and prospects of native pig production and large-scale farming in Laos
155-161Views:799This review aimed to investigate the potential native pig development and the trend that large-scale farming may influence native pig production in Laos. The review found that native pigs were estimated to be around 88% of the total pig population in Laos in 2023. Commercial pig farming was found only in the main cities, particularly the central and southern provinces, which increased from 578 in 2019 to 703 farms in 2022 and 737 farms in 2023. Due to the serious economic crisis starting in 2021, many Lao pig producers stopped their businesses, leading to the decline of the commercial pig population by almost 30% in 2023 compared to 2022. The average pork per capita consumption in Laos in 2021 was estimated at about 14.2 kg. Though not as high as the backyard, commercial farms still contribute essentially to the pork supply in Laos (12%), increasing the volume from 87,628 tonnes in 2015 to 137,775 tonnes in 2024. The main native pig producers are small-scale rural farmers who have not yet improved productivity or reproductive performance, and the main purpose of their production is household consumption rather than market orientation. However, farmers, mainly in the main cities, improved them by cross-breeding native pig sows with Duroc boar for better lean meat. The main challenges for native production in Laos include poor farm setup and management, high cost of commercial feed, and poor breeding and genetic management. Therefore, creating awareness of the benefits of setting up a pig farm and management, providing better option strategies for utilising locally available feeds, and providing better strategies on pig breeding could improve native pig production in Laos.
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The impact of cultivar and irrigation on yield, leaf surface temperature and SPAD readings of chili pepper
103-108Views:487Paprika is an extremely popular and widespread plant species in Hungary. Chili peppers belongs to paprika and it can be consumed fresh or processed. The production of peppers began with the cultivation and control of some parameters for good results of paprika production. The aim of this article is to compare the water supply of four chili pepper hybrids ("Habanero", "Hetényi Parázs", "Unikal" and "Unijol") by means of investigation on open field and to examine how different temperatures and water stress factors influence the yield of chili peppers. The experiment area consisted of four different hybrids in three different irrigation treatments (control, deficit- and regular irrigation). The chlorophyll content was estimated with SPAD and the leaf surface temperature with Raytek MX4 TD. Our research concluded that deficit irrigation produced the highest yields for all cultivars and the lowest yields for the highest water supply.
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Testing of Various Solvents for the Preparation of Lipid Samples for Use in Thin Layer Chromatography
49-50Views:172We analyzed the muscular tissue of 6 sheep. Lipid extraction was performed using the Handson and Olley method. TLC separation was performed using 4 separation systems and the best separation method was determined. Visualization was done using a mixture copper acetate and phosphoric acid, at 150 oC.
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Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles
33-39Views:413Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the organic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.
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The influence of the fertilizing system on the petroleum residues biodegradation on a preluvosoil under control polluted
251-254Views:223The paper presents the partial results of researches regarding the agrochemical melioration of soils under control polluted by petroleum residues that took place at the Agricultural and Research and Development Station in Oradea, Bihor County.
The experimental device was made out of 1 m2 microparcels, spread out in subdivided parcels, in four repetitions, having tree factors: A - the pollution by crude oil from Suplacu de Barcău, B – the mineral fertilization, and C – the organic fertilization.
The experience was set out on a preluvosoil în the year 1993 and the soil was cultivated with millet in the first 3 years and with spring wheat in the last 7 years of research.
The researches carried out in Oradea had the objective of establishing the effects of the fertilizing system on the petroleum residue biodegradation on a preluvosoil under control polluted with crude oil from Suplacu de Barcău, Bihor County. -
The potential use of Pediococcus spp. probiotic in aquaculture: A review
99-106Views:612Aquaculture production has significantly increased over the previous few decades. However, antibiotics have been implemented regularly and extensively to overcome outbreaks of pathogens in aquaculture to cover the human needs for animal protein, leading to the appearance of resistant strains that may cause serious damages in the environment and also human health. In the last few years, the implementation of probiotics as an alternative technique to antibiotics use in fish production has achieved promising results in aquaculture due to their beneficial impact on fish health and growth performance. Among different types of probiotics, the Pediococcus spp. bacteria stand out as a promising probiotic for their beneficial impact to aquaculture. Thus, the current study has been conducted to give an overview about the interactions between Pediococcus spp. and aquaculture. In addition, this review highlights the role of Pediococcus spp in promoting growth performance, improving feed conversion ratios and the intestinal architecture, enhancing the immune response and inhibiting fish pathogens, thereby preventing or at least reducing the use of antibiotics. Practical use of Pediococcus spp. probiotic in aquaculture as feed additives through selected case studies is also considered.
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Comparative study of special honey products and herbhoneys
117-120Views:815Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
Understanding leptin’s influence on calf development: A review of metabolic and reproductive mechanisms
145-153Views:621Leptin, a hormone primarily produced by adipose tissue, regulates the energy balance, metabolism, and reproductive functions. This review explores leptin's role in calf development from embryogenesis to puberty. Leptin expression is strongly influenced by the nutritional status, with circulating levels dependent on adipose tissue mass and modulated by metabolic hormones such as insulin and glucocorticoids. It plays a regulatory role in the embryonic cell division, adipogenesis, and postnatal energy metabolism. Additionally, leptin modulates appetite, the growth hormone secretion, and the reproductive system maturation. Its presence in the colostrum and milk highlights its significance in the early calf development, influencing the neonatal metabolism and immune function. As calves approach puberty, leptin serves as a key metabolic signal for reproductive maturation. A deeper understanding of leptin’s multifunctional role in calf development could contribute to improved livestock growth and reproductive management strategies.
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Heterobeltiosis and combining ability among Egyptian cotton genotypes under well irrigated and water deficit conditions
5-18Views:337Two field experiments were carried out during the 2014 and 2015 seasons to evaluate certain genotypes of Egyptian cotton under well irrigated and water stress conditions. The cross combination Giza 69 x Australy recorded the best values for better parent heterosis for all physiological measurements and earliness index under well irrigated and deficit conditions. Of the female parents, Giza 86 was found to be a good general combiner for most yield characters under both normal and stress conditions, followed by Giza 94. Data illustrated that Giza 45 was the best general combiner for most fiber quality traits under both conditions. The cross combinations Giza 86 x Dandra and Giza 69 x Pima S6 showed significant positive SCA values for seed cotton and lint yield/plant under well irrigated and water deficit conditions. However, the combinations Giza 77 x PimaS6 and Giza 94 x 10229 recorded significant positive SCA effects for seed cotton yield/plant under stress. Whilst, the cross Giza 68 x 10229 recorded significant desirable SCA effects for most fiber quality properties. In this context, the estimates significant positive general and specific combining ability effects indicated that the epistasis and/or dominance effects for F1 hybrid in cotton could be important to a certain extent.
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Testing the antimicrobial activity of essential oils
71-74Views:272The vapor phase of some essential oils proved to have antimicrobial activity. Utilization of the vapor phase of Eos is presently understood as one of the possible alternatives to synthetic food preservatives which could be used in the future. However, testing the vapor phase of EOs against microorganisms causing food-borne diseases (e.g. Salmonella enteritidis or Staphylococcus aureus) or food spoilage is relatively new. Consequently, due to the large number of known EOs, research on their antimicrobial activity is still largely in the phase of in vitro rather than in vivo testing. Moreover, no standard and reliable method for fast screening of a wide range of samples exists. Thus, the aim of this study is to show results concerning tests of the antimicrobial activity of EOs against S. enteritidis or S. aureus, which were conducted by two modifications of the disc volatilization method we developed. The lately developed method has the potential to become widely used for fast screening of EO antimicrobial activity in the vapor phase.
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The effect of breed and stage of lactation on the microbiological status of raw milk
37-45Views:701The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.
There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.
Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.
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Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA
29-32Views:666Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.
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Growth and yield patterns of black locust (Robinia pseudoacacia L.) sample trees affected by site conditions: case studies
125-128Views:334The trees removed from the long-term experiment plots are available for the measurements as lying trees. Through the determination of the volume in sections along the stem, the stem form, the stem volume and other factors can be specified. The comparison of the stems of individual trees of first and third yield classes of black locust (Robinia pseudoacacia L.) stands shows that site conditions have a main effect on the yield (mean tree volume). The difference can be as high as 53% at the age of 30 depending on the sites. To determine the growth patterns based on tree volume is rather a new approach in the light of the relevant literature. The obtained results also highlight the importance of choosing the appropriate tree species for a given site.