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Paradigm shift in the area of specialised social care in Hajdú-Bihar county
253-257Views:90This paper tries to find the answer through an already working practical example how to save money on the social supporting system maintenanced by an elderly society. With the help of basic structural and organizational rearrangement, the social institutions, run by settlements and local goverments, can be helped to ran better and gain savings at the same time.
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60th Anniversary of Hungarian Plant Protection Service
8-10Views:126The author briefly overview the history of the 60-year-old Hungarian Plant Protection Service, which has improved to a model by its ’golden age’ period with a well organized system and excellence of specialists on the chemistry period of Hungarian agriculture. The profound changes both in policy and economy resulted a serious structural changes which continue even nowadays. Meanwhile country borders have been opened than EU membership of Hungary have generated new challenges by appearance and spread of new pests.
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Evaluation of harvesting technology of vineyard pruning based on a Mátra wine region case study
91-100Views:116Wineyard pruning utilization for energy purpose is not only a theoretical possibility, the machine background has also been developed. Economic- and environmental experimentations has made by specialists and they seek to developed the best practice in logistics suitable for local conditions and they propagate the results for the potential users. Nevertheless, the utilization does not seem to be typical in Hungary and some other wine-grower countries. For example, in Hungary the additional energy from vineyard pruning eventuates – tillage, nutrient supply; – phy+tosanitary, environmental pollution; – energy management and economic questions.
In Hungary the most important problem is practice of the vineyard pruning utilization were mentioned by the users is the establishment of collection system and the high logistic costs as Marczinkó (2007) experiences confirm this. As I experienced in practice, the winegrowers are uninterested in utilization. Most of them burn it at the end of the vineyard in many cases without considering of the relevant statutory prohibition.
As my own several years expriment shows at Mátra wine region it is not the technical background which causes the failure. We can use effectively balers or chippers for collection. The cost of chipping is 14 535–27 000 Ft per hectars with the introduced technologies on Mátra wine region. The cost of 1 GJ of heat production is 606–1125 Ft. We can substitute the fuel with vineyard pruning and it means approximately 115 000 Ft saving for a family household per year.
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Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
253-265Views:288Agricultural management practices – directly or indirectly – influence soil properties.
Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization doses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).
The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.
With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds
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Awareness and adoption of a nurse sow management system among small-scale pig farmers in Nakuru County, Kenya
113-120Views:84This study aimed to assess the level of awareness and adoption of nurse sow management strategies among small-scale pig farmers in Nakuru County, Kenya. The research included a survey of pig farmers who visited the Nakuru Agricultural Show in July 2023. A total of 139 farmers were interviewed within 5 days of the show. The corresponding author interviewed the respondents in a face-to-face engagement, where questions on nurse sow management were initially drafted in English and translated into Kiswahili, i.e. the second language in Kenya. The obtained results indicated that farmers came from two main regions of the county: the northern part (54.7%, 76/139) and the southern part (45.3%, 63/139). Levels of awareness and adoption were insignificant between the two groups (χ2, p<0.05). A major obstacle to the adoption of this strategy was identified as feed challenge at 77.7%; (108/139) and market issue at 59.7%; (83/139). This study identified important aspects and limitations that should be considered when developing sustainable productivity development strategies for Kenyan pig farmers. To assist the sustainable growth of small-scale pig production, the authors suggest government measures that shield farmers against exploitation of feed and marketing components, as well as advocating for effective breeding to increase live born.
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Fruit quality parameters of sweet cherry cultivars produced under rain protected plastic foil and general orchard conditions
66-69Views:80Due to global climate warming, frequency of negative weather effects (rainfall amounts, distribution, sortness) are increasing. Rainfall
amounts and frequency has also great effect of sweet cherry fruit quality around fruit ripening. Determination of optimal technological
basics (such as first class fruit quality and economic value) are an important task in dynamically growing sweet cherry production. This can
be solved with introduction of a new intensive training system. One of the solutions can be rain protecting foil which can reduce fruit
cracking and fruit rot. Without this option sweet cherry can not be grown in many countries. In this study, fruit quality parameters were
compared from a 10 year old intensive (4 x 1m) sweet cherry orchard. The effect of rain protecting foil was tested in comparison with fruits
from not covered tree -
Connection Between Leadership and Communication
248-255Views:73Discussing communicationis not an easy job as it is about one of the most important symptomps of life, which plays an important role in all social humanic processes. Ways and devices of communication, communicational system of society developed – while in constant connection – with daily activity, social relations, bodies and structure of society.
Communication takes a prominent part in all forms of management. By the help of this article I would like to demonstrate what importance communication has according to literarure and what relationship can be experienced between communication and management. Is D’Aprix statement reliable saying “communication is management or more properly drafted management is communication”?! -
Clay-pit systems fishfaunistic research in the Middle-Tisza
81-92Views:72After the regulation of the Tisza River the chance of successful fish propagation lessened. Natural spooning places in the river almost completely disappeared. The fish have to find an adequate place for their propagation in the flood plain. The period of spooning usually coincides with flooding of the river. At this time fish try to find the flooded shallow places for spooning. These parts mostly include the clay-pits beside dams, which were accidentally established during construction of the dams. At this place the fry can find the necessary food. After decreasing the flood the fingerlings and a part of the spooners are trapped inclay-pits as these latter ones are not connected with the river bed. The clay-pits usually desiccate during the summer. The trapped fish population is eaten by water birds or harvested by the local man population. This means a great loss for reproduction of some fishspecies.
Within the framework of the Regional Rehabilitation Program at Nagykörű, supported by the “WWF Hungary” and a “SAPARD project,” these clay-pits became connected with each other in a stretch of 5 km, and they were jointed to the Tisza by a collecting channel. The water level has been regulated by a flood gate so that the water enters the holes during the flood and is released later on in to the water bed.
Data on fish were collected from the Nagykörű Whole System, from the Anyita pond and some isolated wholes in Szandaszöllős in 2004 and 2005. First of all fish fry and fingerling were collected and the success of spooning at these places was examined. Fry was harvested by a 60 x 80 cm sized lifting net of 2x3 mm mesh size. In other cases nets used by anglers for catching prey fishes was also used. Data were also collected from local fishermen who participated in saving the fry and fishing of Anyita pond.
Of the several thousand caught fish specimens 28 species were identified, and among them 5 protected and 5 economically important species was found. Protected fishes were as follow: gudgeon (Gobio gobio), bitterling (Rhodeus sericeus amarus), weather fish (Misgurnus fossilis), spined loach (Cobitis elongatoides) and tubenosed goby (Proterorhinus marmoratus). Among economically important fishessamples of asp (Aspius aspius), carp (Cyprinus carpio), wels (Silurus glanis), pike (Esox lucius) and pikeperch (Sander lucioperca) were -
Interactions of the scientific results and practice in the biodiesel plant in Mátészalka, in the eastern tri-border area of Hungary
23-27Views:84This study summarises the observations about the operation of the first Hungarian model-like biodiesel plant (which has a producer background). In order to efficiently and safely operate the applied technology and to produce biodiesel that conforms to international standards, it was indispensable to apply the scientific results in practice and to develop a cooperation with the University of Debrecen (Centre of Agricultural Sciences and Engineering). The biodiesel plant has a significant importance from the aspects of the Eastern tri-border area and the North Great Plain region.
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Challenges and limtations of site specific crop production applications of wheat and maize
101-104Views:129The development and implementation of precision agriculture or site-specific farming has been made possible by combining the Global Positioning System (GPS) and the Geographic Information Systems (GIS). Site specific agronomic applications are of high importance concerning the efficiency of management in crop production as well as the protection and maintenance of environment and nature. Precision crop production management techniques were applied at four locations to evaluate their impact on small plot units sown by wheat (Triticum aestivum L.) and maize (Zea mays L.) in a Hungarian national case study. The results obtained suggest the applicability of the site specific management techniques, however the crops studied responded in a different way concerning the impact of applications. Maize had a stronger response regarding grain yield and weed canopy. Wheat was responding better than maize concerning plant density and protein content performance.
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Contexts between apple orchards with various cultivar comparisons and the effect of ATS (ammoniumthiosulphate) on fruit thinning
35-38Views:143Our research focuses on a fruit thinning material that can also be used in apple production. This material reduces significantly the required manual labor of hand thinning by russeting the blossoms selectively. The ATS (ammonium-thiosulphate) acts as chemical desiccant contrary to the nowadays commonly used materials such as naphthalene acetic acid, naphthyl acetamide, benziladenin and ethylene, which affect the metabolic processes of the plant by regulating the hormone system. In our experimentals cultivar ’Pinova’ and ’Golden Reinders’ were treated with different concentrations of ATS. The effect of these doses on the fruit setting and the quality and quantity parameters of the fruits was studied. According to our results, in the case of cultivar ’Pinova’ the ATS did not have any detected effects at the concentration of 1.5%. Application of ATS at 3% decreased considerably the fruit setting and fruit yield, accordingly the mean fruit size improved. The response to treatment in the case cultivar ‘Golden Reinders’ does not have any similar consistent results.
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Let's talk about 'One fungus One name'. Changes in the fungal taxonomy
11-14Views:213Mycologists have recorded a few hundred thousand Latin names for fungi and these are thought to refer to 70 000 or so separate species. The use of molecular techniques in fungal taxonomy and systematics in the last 25 years has provided massive amounts of information to clarify phylogenetic relationships, encouraged grant support, and complicated the jobs of classically-trained mycologists. Taxonomists have a reputation for being traditionalists, but the community has recently embraced the modernization of the nomenclatural rules by discarding the requirement for Latin descriptions, endorsing electronic publication, and ending the dual system of nomenclature, viz. teleomorph and anamorph names, which used parallel for the sexual and asexual phases of pleomorphic species. A group of taxonomists accepted ’The Amsterdam Declaration on Fungal Nomenclature’ and its basic principle the ’One fungus – one name’ has been incorporated in the Code of Nomenclature (’Melbourne Code’) in 2011. The next, and more difficult step will be to develop community standards for sequence-based classification. As the’One fungus – One name’ theory is a brand-new issue for the Hungarian plant doctors and practical specialists, it seems reasonable to review this to promote conversations between generations in Hungarian language.
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System of relations between competitiveness and social cohesion in the European Union (2007–2020)
39-45Views:131I my current essay I tried to prove that the European Union modified its economic policy due to the financial and economic crisis and the fierce global competitiveness requirements. The main emphasis was laid on the increase of competitiveness. Competitiveness became preferred
to cohesion and the economic and social closing up of the newly joined Middle-Eastern European countries. The funds for competitiveness for growth and employment increase by 6–7% yearly during the financial perspective between 2007–2013. On the contrary the funds for agriculture and rural development decrease by 3% yearly in this period. The tendency remains unchanged during the financial perspective 2014–2020.
This tendency strengthens the establishment of the two speed Europe concept and causes tensions between the core regions and the peripheries. -
Does the feeding frequency influence the growth performance of European perch juveniles (Perca fluviatilis) during intensive rearing?
123-128Views:140The European perch (Perca fluviatilis) is a predatory fish species. Its aquaculture production is increasing worldwide. Feeding and the frequency of feeding are important elements of intensive fish rearing. The aim of our experiment was to examine the optimal distribution of the amount of feed, at the same feed rations. The experiment lasted 42 days. Three treatments were applied in 4–4 replications. The first treatment was feeding twice per day (T2), the second treatment was feeding three times a day (T3), and the third group was fed four times a day (T4). 10 European perch juveniles were stocked per tank, with an individual mean body weight of 3.93 ± 0.06 g at the start of the experiment. The survival rate (S%) was above 90% for all treatments. The T2 treatments produced the most favourable harvest weight (13.96 ± 0.14 g) and specific growth rate (SGR = 3.08 ± 0.01% day-1), but no significant differences were observed between groups. In terms of feed conversion ratio, the best result was obtained by (T3) (FCR =1.06 ± 0.18 g g-1), but no significant difference was found for this indicator neither. The results of the trial indicate that the feeding frequency does not influence the production parameters.
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Economic impacts of applying EU animal protection regulations in hog breeding farms
76-80Views:87Hungary’s intention to join the EU makes it necessary to adopt, introduce and use the EU system of law. In Hungary, the legal control of animal welfare has improved (XXVIIIth law in 1998); however, most of our pig farms do not meet the EU animal welfare law requirements for some reason. We examined 9 pig farms in Hajdú-Bihar, Borsod-Abaúj-Zemplén and Heves counties. We chose those farms which use the combined breeding
technology most frequently used in Hungary. The most important part of the welfare directive is the definition of the minimum space per animal. We analysed the data in comparison with EU laws. On the basis of the analysis, it can be said that there is a narrow cross-section: the breeding of piglets. During the cost analysis, we analysed cost and highlighted the permanent cost. We studied how these costs would change if EU animal welfare laws were observed. We also examined the specific data per 1 sow and per 1 kilogram of slaughter pig. We compared the present data (1999) with those we get if EU animal welfare laws concerning minimal space per pig were now followed. It can be stated that after decreasing the sow live-stock in accordance with EU directives, the permanent cost would increase by 17,7% per sow. If the required space per sow were provided, the total cost per sow would increase by 1,9% from 421,1 thousand forints to 429 thousand forints, on average. This would mean a decrease of 7,9
thousand forints profit per sow. As with the decrease of the number of sows, the number of slaughter pigs also decreases. Total cost per 1 kg of slaughter pig would increase from 214,7 forints to 218,2 on average. -
The Target Areas of Regional Development, Tool and Institutional Background, its Present and Future Prospects
138-142Views:72Regional politics determines the aims of regional structures, and the tools with the institutional background on the whole necessary achieve these. Realizing the aims of regional politics is a precondition of executing the basic principles of regional development and politics. The regional institutional system for handling the decentralized financial resources has not evolved in Hungary yet. The professional institutes for preparing the programs have not been set up, and the management of execution is slowly taking shape. There are still deficiencies in the domestic application of concentration and addition principles. A number of funds are provided on a national level, that are directly or indirectly aimed at regional development. The obvious coordination among the funds is not carried out neither on a national, nor on a regional level, partly to avoid parallel consumption aims. The future of Hungarian spatial structure depends on the type of decentralization strategy the country will follow for the new resources after the accession. Total decentralization offers the most efficient solution for Hungary.
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Study of The Amount of Produced Animal Manure and Biogas Potential in a 5, 20 and 40 km Zone around Debrecen
64-68Views:75The guiding principle for our research is that decentralized energy supply systems should be located near local consumers. One big, local market for energy from biogas plants in Hajdú-Bihar county is Debrecen. In the course of the investigation of biomass suitable for biogas production in the area of Debrecen, this study determines the quantity of biogas producible from animal manure. Municipalities around Debrecen were examined in circles with 40, 20 and 5 km radius. Livestock data of these settlements stem from the Agricultural Census in year 2000, actualized by the county trend of livestock changes. Manure quantity and biogas potential in these places are estimated with coefficients from the literature and our calculations. In the largest circle, about 1.4 million tons of manure is produced, from which round 94 million m3 biogas can be produced. Biomass produced in the settlements and producible biogas shows a remarkable trend of concentration, which supports our recommendation on the system of energy supply.
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Globalization of Services in Practice. (Analysis of Success on Polish Example in the Years 2003-2011)
67-71Views:102The globalised world is looking for savings and actually finds it in Poland. Global economy is a system of connected vessels, functioning in close relations, with increasing role of Poland. The text demonstrates the developmental perspectives of outsourcing and offshoring in the largest Polish metropolies and the possibilities of the development of smaller cities.
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Application of hydrological models for agri-environmental management based on international and national experiences
45-50Views:128The ecological restoration of surface water flows requires the use of complex GIS and hydrological models. The HEC -RAS and MIKE11 programs calculate the hydraulic characteristics of runoff using the geometry and bed resistance the of flows. The presentation of some possible applications made through an example of the Berettyó, which is an extremely regulated river. The river belongs to the meandering category by Parker's classification system, which uses the relationship of the slope-Froude number and the depth- width ratio. Three coefficient of the four determining factor (entrenchment ratio, slope of water level, width-depth ratio) also indicates a mediocre to high sinuosity, while the river regulations established nearly straight bed-line. The standard water flow rates and characteristics have been determined based on the modeled Froude and Reynolds numbers. The flow conditions shifted to the laminar type by the changed line drawing which is ecologically lower status. Based on the results the morphological and ecohydrological river rehabilitation is appropriate.
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Harvesting system established for the utilisation of Miscanthus sinensis ‘tatai’ “energy cane” in biomass power plants
143-150Views:145The increasing demand for energy worldwide and the resulting environmental impacts of fossil fuels forced many countries to turn to renewable energy resources as a clean and sustainable alternative. More than a third of Europe’s binding renewable energy source target of 20% by 2020 will come from solid biomass for electricity and heating according to the National Renewable Energy Action Plans submitted by member states of the European Union (EU) to the European Commission. To achieve this goal long-term yield studies in renewable energy plants are important to determine mean annual biomass and energy yield, and CO2 emission. Field experiments worldwide and also in Europe have demonstrated that Miscanthus, a fast-growing C4 rhizomatous grass can produce some of the highest biomass and energy yield per hectare of all potential energy plants. Miscanthus is a plant that originates from the southern slopes of the Himalayas. It was bred for the Hungarian climatic conditions in 2006 under the name of Miscanthus sinensis ‘Tatai’ (MsT). The species has high frost and drought tolerance and high energy value. This is why there is growing demand for the biomass (lignocellulose) produced by growing this plant. The biomass, produced from the high yield energy reed, can be transported to power plants in large quantities, in forms of bales. Its household consumption is not yet significant. This study presents the external features, characteristics, propagation and plantation process of MsT energy reed. The study also demonstrates the harvest technology of the species worked out between 2009–2012 in Tata, Hungary and the options of supplying to biomass
power stations.
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Failure causes and effect analysis of water supply systems
209-214Views:60The present paper intends to overview the problems of the failure causes and effect analysis of water supply systems. The author’s efforts were channeled to systemizing, classifying and adapting the content to the hydraulics engineering field.
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Dimensionality Expressed by Caseendings and Spatial Prepositions
7-15Views:80The purpose of this essay is to investigate some of the uses of English prepositions and Hungarian case endings employed to express spatial relations. The observation of invariant mistakes Hungarian native speakers learning English make initiated the investigation. The questions raised are: (a) where do the two systems match and where do mismatches lie, (b) how do language users perceive the world, and (c) do speakers observe spatial relations as two-dimensional or three-dimensional cognitive models? Do different languages see the same thing as either three-dimensional, or two-dimensional?
Abondolo (1988) gives an adequate morphological analysis of ten Hungarian case-endings (inessive, illative, elative, superessive, delative, sublative adessive, ablative, allative and terminative) used in spatial reference, which give a closed set in references made to factors, such as (1) location which can be broken down as interior vs. exterior location with the latter being further analysable as superficial and proximal, and (2) orientation which can be analysed as zero orientation (position), source and goal. In addition to those in this list, two other case endings (genetive/dative and locative) are also used for expressing spatial relations but the last is only a variant of the inessive and superessive case-endings and is only used with place-names. The set is closed in the sense that the same item is meant to refer to the same sort of spatial relation in every case. Language textbooks, c.f. Benkő (1972) seem to suggest a neat match between the above Hungarian case endings and their English prepositional counterparts, e.g. London-ban (inessive) = in London.
The picture, however, is far from being so clear-cut. The data, which were taken from various dictionaries and textbooks, show that the choices of both the prepositions and the case endings listed above depend on how the speaker considers factors (1) and (2) and that proximity is very important. Instead of a one-to-one match between the prepositions and the case endings, we rather find that the above case endings will match a dual, and in some cases a tripartite system of prepositions with the correspondences found in the two languages, which yield the following chart:
We suggest that languages may view or map the same physical entities in different ways, for example along surface vs. volume or goal vs. passage, etc.
Furthermore, we also find it possible that it is the language specific, inherent coding of the nominal phrase that decides – in many cases – upon the choice of prepositions and case endings. -
Research on rheological characteristics of winter wheat varieties
266-272Views:140The gluten examination test is dominant in Hungary, comparing it to the qualification system of other countries. The determination of alveographic parameters is a basic criterion of winter wheat specifications in some western and southern European countries.
In the Hungarian and foreign winter wheat qualification systems, in the standards, there is no limit for extensographical parameters. Customers dictate the limits and make their claims as to the specifications.
We analyzed the alveographic and extensographical parameters of 19 winter wheat varieties grown by the Cereal Research Non-Profit Company in Hungary, and we made a comparison between the results in the challenges of the European Union expectations.
Examining the information of alveographic and extensographical values, we found that the experimental varieties provide high base to flour types suitable for baking bread and baker’s ware. The GK Élet, GK Petur, GK Memento, GK Csillag, GK Kapos and GK Marcel varieties can meet the market of paste flour needs, too.
In the variety series, the GK Kalász represents the highest values and the GK Garaboly shows the lowest parameters regarding the alveographic W and the resistance to extension.
On the basis of relation, we can establish that we can estimate the following little known and used quality indexes: the extensographical resistance to extension with extensographical energy and the alveographic W value, the extensographical energy with the alveographic W value and the alveographic P and L values with the other alveographic parameters. -
Presentation of sales price reserves for live lamb
37-45Views:89Although sheep breeding in Hungary is grounded in strong traditions, its activities only comprise 1% of the total production value generated from agriculture, and 2% of that for animal-based products. The most significant portion of incomes earned in the Hungarian sheep sector has, for years now, stemmed from the sale of live animals. The sector is decidedly export-oriented, as the domestic demand for its main product, i.e. lamb meat, and is minimal, equaling some 20-30dkg per person per year. Part of the sold animals is sold to market as dairy sheep, while the greater
part is sold in the category of lamb carcasses. For this reason, the average weight of slaughter sheep has lied between 19-22 kg for years now. The target markets for live lamb sales are predominately Italy and Greece. In Greece, movement on this market has shown an upward tendency in recent years; noteworthy are also sales to Austria, Holland, Bosnia-Herzegovina and Poland. In 2003, we exported sheep meat in the form of carcasses to Italy, as well as to Germany, and of these exports, 94% went to Italy, while the remaining 6% went to Germany.
Among sheep products, only the trade balance for live animal sales is positive. But even for such producers, only those who are specialized in intensive breeding and those sheep farms “targeting” meat production can obtain earnings. Specialized literature on the sector argues that the quality of Hungarian lamb has diminished dramatically and is beginning to lose its market position. If Hungarian lamb does not meet market expectations, then it will only be bought from producers at lower prices.
My research focuses on those factors which influence price. I separately discuss the question of quality, as one of the most important decisive factors on price. Within this discussion, I describe the market expectations which actually have an effect on the acceptance of live animals for sale on commission. In practice, after the problems of quality, the next most important question is that of when sale is made. In the course of my research, I studied the development of averages for sales on commission of live lambs using statistical methods. The most important problem of this sector is the hierarchical system used in sales: this is the topic hich neither the producer, nor the buyer, wish to discuss, not even with each other. On the basis of the information at my disposal, I outlined the levels of traders and individual levels used to arrive at commission prices. Finally, I examined the components of the production value of live lamb sales. The results I obtained quantified the key role of the prices and the yield, as well as the factors influencing income. -
Detection of DNA mutations by PCR-TTGE method
21-25Views:179In our study PCR-temporal temperature gelelectrophoresis (TTGE) and MeltINGENY bioinformatic program were used to analyse the mutations in the genes of melanocortin-1 receptor (MC1R) and pituitary adenylate-cyclase activating polypeptide (PACAP) in cattle. Amplification of target DNA by PCR was performed with GC-clamp primers and non-GC-clamp primers in simplex PCR reactions. The fragments were separated by denaturing polyacrylamide gelelectrophoresis (denaturing agents: high temperature, urea) after PCR reactions.MC1R homozygous individuals were used for the reaction.
We concluded that MeltINGENY program makes the decision and detection system easier, and more simple as the melting profile of target sequence is determined by the software. In case of MC1R gene, PCR-TTGE method is appropriate for SNP detection, however PACAP gene polymorphism can not be identified by the method, because PACAP mutations are not included in melting domains, therefore PCR-TTGE cannot detect them.