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Assessment of Environmental Susceptibility/Vulnerability of Soils
62-74Views:247Soils represent a considerable part of the natural resources of Hungary. Consequently, rational land use and proper soil management – to guarantee normal soil functions – are important elements of sustainable (agricultural) development, having special importance both in the national economy and in environment protection.
The main soil functions in the biosphere are as follows: conditionally renewable natural resource; reactor, transformer and integrator of the combined influences of other natural resources (solar radiation, atmosphere, surface and subsurface waters, biological resources), place of „sphere-interactions”; medium for biomass production, primary food-source of the biosphere; storage of heat, water and plant nutrients; natural filter and detoxication system, which may prevent the deeper geological formations and the subsurface waters from various pollutants; high capacity buffer medium, which may prevent or moderate the unfavourable consequences of various environmental stresses; significant gene-reservoir, an important element of biodiversity.
Society utilizes these functions in different ways (rate, method, efficiency) throughout history, depending on the given natural conditions and socio-economic circumstances. In many cases the character of the particular functions was not properly taken into consideration during the utilization of soil resources, and the misguided management resulted in their over-exploitation, decreasing efficiency of one or more soil functions, and – over a certain limit – serious environmental deterioration.
Soil resources are threatened by the following environmental stresses:
– soil degradation processes;
– extreme moisture regime;
– nutrient stresses (deficiency or toxicity);
– environmental pollution.
Environmental stresses caused by natural factors or human activities represent an increasing ecological threat to the biosphere, as well as a socio-economic risk for sustainable development, including rational land use and soil management.
The stresses are caused by the integrated impacts of various soil properties, which are the results of soil processes (mass and energy regimes, abiotic and biotic transport and transformation and their interactions) under the combined influences of soil forming factors. Consequently, the control of soil processes is a great challenge and the main task of soil science and soil management in sustainable development.
The efficient control of these processes necessitates the following consecutive steps:
• registration of facts and consequences (information on land and soil characteristics, land use, cropping pattern, applied agrotechnics, yields, with their spatial and temporal variability);
• evaluation of potential reasons (definition and quantification of soil processes, analysis of influencing factors and their mechanisms);
• assessment of the theoretical, real, rational and economic possibilities for the control of soil processes (including their risk-assessment and impact analysis);
• elaboration of efficient technologies for the „best” control alternatives (best management practice).
Scientifically based planning and implementation of sustainable land use and rational soil management to ensure desirable soil functions, without any undesirable environmental side-effects, require adequate soil information. In the last years such data were organized into a computer-based GIS soil database in Hungary, giving opportunities for the quantification, analysis, modelling and forecasting of the studied environmental stresses and for the efficient and scientifically based prevention, elimination or reduction of environmental stresses and their unfavourable ecological and economical consequences.
Special attention was paid to the assessment of various soil degradation processes, as: (1) soil erosion by water or wind; (2) soil acidification; (3) salinization and/or alkalization; (4) physical degradation (structure destruction, compaction); (5) extreme moisture regime: drought sensitivity and waterlogging hazard; (6) biological degradation; (7) unfavourable changes in the plant nutrient regime; (8) decrease of natural buffering capacity, (9) soil (and water) pollution.
The actions against undesirable environmental stresses and their unfavourable consequences are important elements of sustainable, efficient, economically viable, socially acceptable and environmentally sound crop production and agricultural development. These are joint tasks of the state, decision makers on various levels, the land owners, the land users and – to a certain extent – of each member of the society. -
Stevia (Stevia rebaudiana B.) yield in the case of different production technologies
71-77Views:418Stevia rebaudiana B. offers a natural alternative of sweetening, potential health promotion plant, and our country shows increased interest about cultivation in Hungary in addition to the neighboring European countries. The agricultural production system installation necessary
understanding of the needs of the plant, as well as exploring the agronomic potential. Field experiments were conducted in 2015 with the aim to highlight some of the technological production parameters and correlations between them. We have reviewed the most important agronomic factors, the spacing (50×50 cm and 33×33 cm), ground covering (agro-cloth covered and uncovered/ control), their vegetative growth (first-,second-order branch), herb yield and quality in effect.
Based on empirical evidence, that the Hungarian climates also have the opportunity of 3 cuttings during a growing season, besides of early planting, optimal climatic conditions (in October didn’t reduce the daily minimum temperature below 0 °C, after cuttings the maximum interval values remain below 40 °C), and adequate water supply and crop protection facility. The 33×33 cm spacing evaporates more than 50×50 cm spacing, because of the dense population, the continuous canopy less able to breathe, so there are serious chances to the pathogen colonization.
Based on the results of our research to the wider 50×50 cm spacing favorable appreciate the stevia optimal progress in terms of qualitative and quantitative parameters of the yield. The stevia yields produced in the Northern Great Plains field cultivation can produce similar results as stevia crop yields in warmer climates, where the primary crops. Our research experience suggest that there is a viable domestic stevia cultivation, developing the necessary technology is still growing further investigation justifies. -
Effect of hail net on the water potential of an apple orchard
109-113Views:516Apple production has seen a decline in yields in recent years, primarily due to ecological factors such as drought, water stress, water scarcity, uneven rainfall distribution, frost damage and hail damage. Ecological factors that are harmful to the plant can be eliminated by human intervention, irrigation or the use of hail net. In our study, we investigated the positive effects of hail net on the development of plant water potential as the vegetation progressed, in non-irrigation area with temperature and humidity. Water potential values were determined using a field osmometer. Confirmed the positive effect of the ice protection net in the apple orchard of Early Gold and Golden Reinders. Our results were supported by statistical analyzes our results.
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Violation prooxidative-antioxidant stability at maize shoots at different level of accumulation of cadmium and nickel
89-94Views:184Joint influence of cadmium and nickel was investigated on the feature of their accumulation by the vegetative organs of 10-days' old maize shoots. It was established that most intensively noted metals are taken in by the roots of shoots in the first 7 hours stressing influencing, while in leaves they appear only after a 7-hour long exposition. It was stated that the absorption process of the noted metals by a root system is carried by two-phase character. The indexes of inner-tissue contamination are calculated. Activating by the cadmium and nickel ions of lipid peroxidation as marker of the stressing influencing, and also was shown the proper increase of intensity of functioning of ascorbate peroxidase as the antioxidant enzyme protection of cell.
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Previous data on the relationship between the intensity of pruning and the degree of damage in integrated and organic applegrowing systems
47-52Views:276In Hungary, fruit growers are increasingly interested in environmentally friendly growing methods, such as organic and integrated systems. Vital is the establishment of a strengthened system of cultivar-pruning-plant protection in production technology. Consequently, our aim was to examine the susceptibility of apple cultivars to diseases and pests and the effect of pruning technique on diseases and pests in organic and integrated growing systems.
Two pathogens (Venturia inaequalis, Podosphaera leucotricha) and two pests (Leucoptera malifoliella, Pannonychus ulmi) were observed in organic and integrated systems under „strong” and „weak” pruning techniques. Our results on six cultivars showed that the pathogens and pests infested the trees more in the organic system, as compared to that of integrated production. The pruning technique affected mainly the susceptible
cultivars to diseases and pests. The „weak” pruning technique caused a higher level of diseases and pests infestations than the „strong” pruning technique, especially in the organic growing system. The likely reason is that the shoots grow fast and powerfully under “strong” pruning technique. This supports better preservation of the trees supported by susceptibility of plant tissues to diseases and pests. -
The potential of biological control on invasive weed species
73-75Views:327Sorghum halepense is one of the invasive species in Europe. This study was made to identify the morphology of fungi on invasive weed species samples on the roots of Sorghum halepense. The samples were collected in the region of Debrecen. The experiment was conducted under laboratory conditions to determine the microscopic form of fungi. The samples were put on PDA and for identification of fungi is based on the morphological characteristics of the features and colonies of conidia that were developed in Petri dishes.
The examination of the culture revealed that the fungus from the root of Sorghum halepense was Aspergillus niger. Pathogenicity and the relationship between the fungus and Sorghum halepense are still uncertain so in the future pathogenicity tests and re-isolations from plants are very important steps.
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The effect of different compost rates on the yield of ryegrass (Lolium perenne L.)
95-98Views:317Protection of natural resources and sustainable natural resources management are essential for the long-term survival of humanity. This makes necessary nowadays the development of environmentally conscious living and spread of that in the future. The amount of organic waste materials, produced during human activities, could be decreased by composting instead of dispose them in landfills. Applying appropriate treatment technology and additives, the compost could be used as fertilizer for horticultural crops and it could increase the easily available nutrient content of soils. Compost utilization prevents nutrient deficiencies and by using the optimal rate, we could reach significant yield increases.
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Distribution and relative abundance of the economically important click beetles (Elateridae: Agriotes sp.) in Hungary
53-60Views:393Click beetle (Elateridae: Agriotes sp.) species of 24 sites in different regions of Hungary were studied in 2010. A. brevis, A. sputator, A. obscurus, A. lineatus, A. rufipalpis and A. ustulatus were sampled by pheromone traps in maize fields. During the study more than 80000 beetles were caught. The three most common species were A. ustulatus, A. sputator and A. rufipalpis. The distribution of the studied species was uneven. In south Hungary A. ustulatus, A, rufipalpis and A. sputator were the three most abundant species. In the Transdanubia A. sputator was the most abundant. A. ustulatus reached higher abundance in only three sites (3/12). A. obscurus occurred only in west Hungary (Transdanubia). In eastern Hungary the abundance of studied species was higher. In this reason we have to monitor the populations of these pests and if it is necessary we have to take actions against them. In Transdanubia the abundance were generally lower but in many cases reached the threshold of significant damage. Beyond that 13 additional species were sampled so the total number of sampled species was 19.
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Comparison of Scab Warning Systems in Integrated Apple Production
53-57Views:299In the present study, the suggestions for infection risk and sprays of the most frequently used PC-based scab warning systems (RIMpro and WELTE) and the conventionally used Mills table as a reference were compared in integrated apple production. The efficacy of the spraying programs based on the three different warning systems was evaluated by assessment of apple scab incidences during primary infection periods.
13 and 11 Mills infection periods were detected in 2000 and 2001, respectively, during primary infection periods (from 15 March until 5 June). Taking into consideration the heaviness of Mills infection periods, 11 and 9 sprays were applied in 2000 and 2001, respectively. The two PC-based scab warning systems detected 1-4 less infection periods and suggested 1-3 less sprayings compared to the numbers of infection periods and sprays according to the Mills table. Our results proved that the 9-11 sprays according to the Mills table could be reduced to 7-9 sprays by RIMpro scab warning systems which did not reduce the efficacy and did not increase the symptoms of leaf and fruit scab significantly. The WELTE scab warning systems detected generally one or two more infection periods and suggested one additional spray compared to RIMpro warnings and sprays. -
Possibilities of downsizing sweet cherry trees via growing techniques
71-74Views:299By applying smaller crown sizes and intensive growing techniques, many advantages can be identified compared to the extensive orchards. Also, nursing/pruning and harvest work can be performed more effectively. The outer and inner quality parameters of the fruit and the effectiveness of plant protection techniques are improved. The smaller crown size enables us to apply technologies for ensuring yield safety (e.g. hail, rain, bird nets), resulting in an increase in productivity. The introduction of smaller trees poses a great challenge to cherry production. Trials with dwarfing rootstocks have not yet been successful, therefore, we must use the cv. Mahaleb rootstock, which is excellently adapted to the Hungarian conditions, and also has a stronger growth. In addition, rootstocks with such strong growth are needed for the necessary regeneration of the productive parts of cherry cultivars, there is a need for. At the research garden of the University of Debrecen in Pallag, we planted 21 cherry cultivars on cv. Mahaleb (CT500) rootstock, in a 4 m x 1 m spacing pattern, in the spring of 2000. In our study, we demonstrated the possibilities of developing and maintaining the string super spindle through repeated summer pruning, in terms of growth, bud and fruit formation,. Based on these parameters, we determined which cultivars are the most suitable for intensive production.
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Evaluation of the role of common vetch (Vicia sativa L.) green manure in crop rotations
161-171Views:434Common vetch (Vicia sativa L.) is an annual legume, grown as green manure provide rapid soil cover, can increase soil moisture and organic matter content and reduce soil erosion during fall. During the fallow period, legumes grown as catch crops are known by releasing large amounts of mineral nitrogen (N) for the subsequent crop. By taking advantage of these benefits, it is possible to increase the yield of the next crop in an environmentally friendly and sustainable way. The goal of this study was to determine the value of common vetch as a green manure, considering its effect on soil conditions and the yield of next crops. We examined three different common vetch seed rate as a green manure in a crop rotation with triticale, oat and corn. Next to the green manured treatments, we used fertilized and bare fallow control treatment for comparison. In our study we evaluated the aboveground biomass weight of spring vetch green manure and its effect on the moisture content of the soil. We examined the green manure’s effect on the next crops plant height and yield. We found that the moisture content of the green manured plots was significantly higher during summer drought. On the green manured plots, 37.9% higher triticale yield, 50% higher oat yield and 44% higher corn yield were measured compared to the control plots. The insertion of spring vetch green manuring into crop rotations could be a good alternative to sustainable nutrient replenishment methods. It can be used to reduce the input needs of farming, reduce carbon footprint, contribute to the protection of soils and increase the organic matter content of the soil.
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Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil
111-116Views:312In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collected four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
− The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
− The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
− On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment.
− The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition.
− Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources.
Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity.
Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility. -
Testing of paraffin oil efficiency against grape powdery mildew in Eger wine region
73-80Views:596The aim of the present study was to examine the efficiency of paraffin oil against powdery mildew in Eger wine region. The experiment has been carried out in 2013 and 2014 with Chardonnay and Kékfrankos grape varieties, which have different resistance against powdery mildew. The effectiveness of the oil was examined on leaves and clusters (frequency and intensity). This oil was effective against Erysiphe necator infection on field trials in Chile and Brazil. The spread of downy mildew (Plasmopara viticola) was also inhibited by this material in some experiments conducted in Spain and France.
The differences between oil treatments represented the sensitivity of the grape varieties in accordance with the applied dosages. The oil was effective against powdery mildew with different extent as a result of the so called ”vintage effect”. In 2013, the treatment of the highest dosage (D3) didn’t differed significantly in frequency and intensity of infection from the regular treatment (clusters of Kékfrankos, leaves of Chardonnay). In 2014, the oil was not so effective against powdery mildew compared to 2013. No remarkable differences were detected between the treatments due to the strong pressure of powdery mildew. Furthermore, no any effect of the lowest dosage (D1) was detected in the case of the sensitive clusters of Chardonnay and leaves of Kékfrankos in both experimental years.
In summary, the oil treatment has an effect against powdery mildew, however this efficiency largely depends on the vintage characteristics and the pressure of powdery mildew. Further investigations are neccessary, for example field trials with combinations of other sprays. The oil can be useable as fungicide with proper care in eco-friendly integrated and bio (ecological) viticulture.
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Global Issues of RangelandManagement
39-46Views:225Rangelands occupy about 50% of the world’s land area. They are ecologically and economically as important as rain forests and in even greater danger of degradation and disappearance. This paper reviews the definitions and distribution of rangelands and describes their global environmental importance in terms of erosion control, carbon storage and methane emission. Condition and degradation of rangelands are defined and discussed and it is argued that soil protection and carbon storage can be increased and methane emission per animal decreased by conservative use and improvement of rangelands, whilst at the same time alleviating hunger and malnutrition in developing countries. It is concluded that policies should be adopted by national governments and international deve-lopment programs to conserve and improve rangelands.
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The possibilities of biomass utilization in the field of spatial development
59-63Views:188By joining the European Union, Hungary made a commitment to increasingly utilize renewable energy sources. Keeping in view the agricultural circumstances of Hungary, we can state that biomass utilization has, overall, the greatest range of possibilities to use its products as ‘energy sources’. Biomass raw materials are useful in meeting emission control regulations for environment protection and to reduce climate change. The role of biomass production in spatial and rural development and in rural employment, and also in the decrease of Hungary’s energy dependence, supports development in biomass energy use. My aim in studying biomass utilization is the creation of a micro-regional level, decentralised, agro-energetic system-model for the use of plant and animal biomass for energy purposes, whether naturally or by anthropogenic activity, on agro- and sylvicultural areas, and on animal breeding farms. This model simulation method serves as a planning base for policy-makers during the spatial planning processes. The examination is presently in the stage of data collection. The collection of the basic data of the examination area and the purchase of the necessary equipment, materials and maps has already started. Contact has been made with local stakeholders, municipalities, municipality associations and micro-region managers.
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Investigation of the mycelial compatibility of Macrophomina phaseolina in the Carpatian Basin
25-28Views:313Macrophomina phaseolina is a globally widespread fungal pathogen. The fungus has a very wide range of hosts. Under optimal conditions, M. phaseolina can cause serious damage tothe host plants. In this study, the mycelial compatibility of different M. phaseolina isolates was investigated. From 2019 to 2021, 12 sunflower samples were collected from different regions of the Carpathian Basin, 9 samples from Hungary, 1 sample from Austria, and 2 samples from Slovakia. The genetic variability of the pathogen is a critical problem in plant protection. Two compatible pathogen strains can easily exchange their genetic material for each other, which can lead to the development of resistance. All collected samples (12) were tested to examine their compatibility. Isolates from all tested samples were paired with isolates from all other samples thus, a total of 66 pairings were made. During the examinations of mycelial compatibility, only 20 pairs of all possible pairings were found to be incompatible, and all others (46) were found to be compatible.
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Caliometric characterisation of crop production byproducts
55-58Views:254By the decreasing tendency of the fossil energy resources more emphasis put on the usage of renewable energy resources. The consideration of environmental protection and the efforts of the European Union make current the widespread usage of renewable energy within biomass energy. One of the determinative trends of biomass energy is the direct combustion of biomass. Characteristically woody stocks are produced for this aim, but there is a considerable potential in several byproducts of growing herbaceous plants or of other processes.
In our study three byproducts of plant production have been investigated which appeared at the harvest. The Higher Heating Values of wheat, maize and sunflower byproducts have been determined by an IKA C2000 Basic adiabatic calorimeter. According to the statistical analysis of the measured data the HHV of the byproducts are different, and these values are in a negative correlation with the amount of ash in % (R2=0.873) appeared by the combustion.
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Establishing regional cultivating districts on the basis of the Kreybig practical soil mapping system
20-25Views:468With the help of this report evaluating the current situation of the region, characteristics of the development in agricultural production and regional differences can be clarified. By mapping out the regional soil, land use and climatic conditions and organizing these into a geographical information system, one can easily determine which plants are the most ideal to cultivate in that particular region. Moreover, it is a useful tool that enables us to
establish the most favorable land use structure suited to ecological demands and also helps to determine the methods of soil protection.
During our work, we chose administrative units in Szabolcs-Szatmár-Bereg County, based on the latest aspects of regional cultivation.
Our pilot areas are: the small regions of Nyíregyháza, Nyírbátor, Nagykálló, Mátészalka and Csenger.
Using the database, we separated and uncovered the soil conditions of the pilot areas: the chemical and physical properties of the soil layer which is exploited by the roots of the plants, the humus content, the nutrient supply, the thickness of the cultivated layer and the water management conditions.
We separated the districts of regional cultivation, where the basic elements of the traditional Kreybig color systems were applied (light yellow, dark yellow, light brown, dark green, blue, pink, red, gray, greenish brown, reddish purple, light purple, dark purple, light green).
By using the data collected from the pilot areas, we compiled a map database, which is suitable to illustrate the plant cultivating characteristics of the region. We made recommendations to determine the most favorable plants to cultivate in the specific region with the given meteorological and soil conditions, as well as for the shifting of crops.
Our recommendations were also illustrated in a map with a resolution of 1:25000. -
Comparative Vertical Migration of Three Different Strains of Heterorhabditis bacteriophora and a Single Strain of Heterorhabditis megidis in Sand at 25°C
47-52Views:161Heterorhabditis bacteriophora and Heterorhabditis megidis) were tested for downward migration in 9.5 cm vertical sand columns at 25°C with and without a larva of the wax moth, Galleria mellonella, at the bottom. The number of infective juveniles (IJs) in the upper section decreased since the IJs gradually migrated down toward the Galleria larvae into the lower section of the shell vials. Only the isolate OH 25 behaved differently, because the number of IJs decreased this isolate in the lower section. This isolate was the quickest, since after 12 hrs, most IJs could be found in the lower section of the vials from this isolate. The number of IJs was so small in the lower section that 12 hrs after injection, only the isolates MHG 3 and OH 25 could kill the Galleria larvae.
Besides the fact that mortality occurred among the Galleria larvae with the above isolates, neither IJs nor adults could be detected in either living or dead Galleria larvae 12 hrs after IJ injection. It is noteworthy that the behavior of the isolate Megidis was different from the other isolates: the number of IJs was so negligible in the lower section of the vials that even after 36 hrs none of the Galleria died and, consequently, neither adults nor IJs could be detected after dissecting the Galleria larvae. Each isolate could reach the lower section of the vials, yet only the isolate Megidis could not infect and kill the host. After dissecting the larvae, most IJs and adults could be found in the isolate MHG 3 (95 IJs and adults altogether) 36 hrs after injection. -
Comparative analysis of soil analysing datas on different sempling-plots
85-90Views:252Hibrid maize is cultivated on larger plots, therefore the sown areas of hibrid maize are heterogeneous from a pedology aspect. Heterogenity causes problems during tasseling, chemical plant protection and harvest. The heterogenity of sown areas can be compensated by fertilization which is based on soil analysis. We carried out research into change of the soil on four soil types from 1987 to 2005.
There were no significant changes in pH, hydroiodic acidity, CaCO3-content, humus-content on meadow chernozem soil. We detected equalization of salin content in the examined soil layers. There were no significant changes in the measured values on chernozem meadow soil and solonetz meadow soil in 2005. We discoverd equalization of saline content on chernozem meadow soil, but the changes were not as obvious as the changes on meadow chernozem soil. We found salinization in the 30-60 cm soil layer on type meadow soil that may be due to water movement. -
Q-PCR analysis of the resistance of Hungarian Botrytis cinerea isolates toward azoxystrobin
41-44Views:288The genes being in the mitochondrial DNA primarily encode the enzymes of cellular respiration. Fungicides belonging to the family of quinol oxidase inhibitors (QoIs) play on important role in the protection against several plant diseases caused by fungi. These fungicides bind to the cytochrome bc1 complex so they block electron transport between cytochrome b and cytochrome c1. This way these fungicides inhibit the ATP synthesis consequently they inhibit the mitochondrial respiration. The QoI resistance has two mechanisms. One of them is the point mutation of the cytochrome b gene (CYTB), e.g. the substitution of a single glycine by alanine at position 143 results in high-resistance. The other is the cyanide-resistant alternative respiration sustained by the alternative oxidase.
In a cell there are several mitochondria. The phenomenon when the genomes of all mitochondria in the cell are identical is called homoplazmy. If in the cell there is wild and mutant mitochondrial DNA this is called heteroplasmy. Whether the mutation in the mitochondria causes fenotypical diversity or does not depend on the dose, i.e. it depends on the percentage of the changed mitochondrials. During our work we investigated Botrytis cinerea single spore isolates which have been collected in 2008-2009 on different host plants. Our goal was to decide whether heteroplasmy influences the level of resistance. We managed to detect the change of the level of heteroplasmy, so the change the level of the resistance due to the treatment with fungicide. -
Fungi detected in trunk of stone fruits in the Czech Republic
121-127Views:367This study was focused on detection of the spectrum of fungi in the wood of stone fruits using molecular genetic methods. Samples were obtained from apricots, plums and sweet cherry trees from region of Moravia, one sample was obtained from Myjava (Slovakia). Segments of symptomatic wood were obtained from dying stone fruit trees with very significant symptoms. This study describes detection of the fungi in the wood of 11 trees in general in 5 localities. The cultivation of the fungi from symptomatic wood and sequencing of ITS was carried out. Eleven fungal genera were determined in the stone fruits wood, particularly Irpex lacteus, Fomes fomentarius, Neofabraea corticola, Calosphaeria pulchella, Cytospora leucostoma, Phellinus tuberculosus, Stereum hirsutum, Collophora sp., Pithomyces chartarum, Aureobasidium pullulans,Fusarium sp. The results of this study demonstrate that the reason of declining of stone fruit trees in Moravia is caused probably by trunk pathogens.
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Microbiological and Chemical Characterization of Different Composts
106-111Views:160Composting of agricultural waste is considered particularly important from the point-of-view of environmental protection. Degradation of organic substance results in a significant reduction of waste volume.
The end product of the composting process, mature compost, can be used as soil coverage against excess loss of wastes, for mulching, for organic manure etc. The problem of composting has come into limelight in environmental studies and in agriculture.
The quality of the mature compost is determined by physical, chemical and biological parameters of the composting process which, in turn, depend on initial composition of the raw materials, the technology, e.g. regular mixing and moistening and on environmental factors. Quality is the key question in compost use.
We studied the composting process in compost windrows of different raw material composition. We measured temperature, humidity content, pH, organic substance content, nitrogen and carbon content.
We counted the number of bacteria, microscopic fungy, ammonifying and cellulose decomposing microorganisms. We directed the composting process with turning weekly (to provide oxygen) and watering (to provide humidity content 40-60%).
We set up windrows of 1 m3 volume from dry plant substances (cornstalk, pea straw, tomato stalk and crop, weeds) and cow manure not older than 1 week. The cow manure was used at ratios of 0%, 35%, 50%, 65% and 100%, respectively.
We measured changes in compost temperature relationship with outside temperature until they were almoust the same. Humidity was 40-60% in most cases.
At the beginning of the process, pH was slightly acidic-neutral; it later becomes neutral-slightly alkaline (pH: 6.93-8.02) as ammonia is liberated from proteins.
At the end of the process, pH decreased again, due to humification.
Organic substance content decreased as microorganisms mineralized them. Organic carbon content decreased gradually due to microorganisms used it as an energy.
Total nitrogen content increased until middle of july and decreased gradually until than.
The carbon/nitrogen rate were higher in the beginning, it decreased until july-august and increased by smaller degree until end of the process.
The number of bacteria was higher in the first three weeks and between june-september. The number of cellulose degrading bacteria was the highest in the first three month, the number of ammonifying bacteria was the highest from the end of may until sepember.
The number of microscopic fungy was significant in the second part of process, after july. -
Occurrence of woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) in Hajdú-Bihar county, Hungary
119-123Views:188Woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) is native to East Asia, it spreads in several parts of the World and causes difficulties in plant protection, especially in maize. Difficulties in control of Eriochloa villosa originated from several reasons: seeds continue to germinate later in the season, significant part of seeds emerges from a deep layer of the soil, and the species is less susceptible to some herbicides applied to maize than other annual grass weeds.
The first report on the occurrence of woolly cupgrass in Hungary was published in 2008, and it reported about the appearance of this species near to Gesztely village (Borsod-Abaúj-Zemplén county), however, no information has been added about spread of the weed in Hungary until now.
A significant population was discovered next to Debrecen (Hajdú-Bihar county) in summer, 2011, and then weed associations were examined in maize, sunflower and stubble-fields on several km2 in the area to estimate the Eriochloa villosa infection. The weed species was found on every maize field bordering with a ground cover of 0.5-4%. Woolly cupgrass occurred inside of the 50% of maize fields, and reached a ground cover of 76% in case of most infected area, in addition it was found in sunflower and stubble-fields.
The spread of woolly cupgrass is expected in this area, which requires the consideration of this species in the planning of weed management technologies. -
Possibilities of ecological farming in the North Great Plain Region
69-71Views:186Agriculture plays a significant role in the North Great Plain Region. Organic farming is a sustainable farming method controlled by regulations. Active environmental protection and conservation play significant roles in organic farming, along with food quality and safety, originality and the monitoring of products. Organic farming offers opportunities to create employment, social safety and high profitability. As a result of these characteristics, organic farming could become one of the most important means of developing our region in the future.