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  • Fresh fruit purchase analysis in the town’s circle in the Nothern Great Plain Region
    101-106
    Views:
    251

    In my research I aimed to examine the previous theories about consumer habits and behaviors as well as the preference of different fruit species in the Northern Great Plain Region. Furthermore, I also wanted to know if there are any correlations between the product atributes that influence fruit consumption and different ages, genders and income categories.

    The importance of my research field is reflected in the fact that issues in connection with foodstuffs, healty diet and food safety have been highly emphasized recently.

    I belive in order to live longer we need to pursue healthy diet that is rich in fruits and vegetables. It seems, however, not every can follow this lifestyle in their every day diet since most people prefer junk food that contains harmful ingridients.

  • An overview of swine production and marketing in Africa - Mini review
    121-129
    Views:
    678

     Pigs are the oldest domesticated animals, though their ancestry is still uncertain because of a lack of archaeological and genetic evidence. Pigs serve a vital role in reducing the demand for livestock and livestock products in most parts of the world. Especially in the African continent, pigs serve as mobile cash implying how easily they can be converted into cash to sort pressing family needs. Pig production in Africa is different from advanced systems of production in temperate countries, however, it has similarities with most of the tropical low and middle-income nations. Pig production in Africa requires attention to enable it to contribute effectively to the growing African population. However, it has been orchestrated with a myriad of challenges including market access. This mini-review is designed in a manner to provide an overview of pig production contribution to livelihoods in Africa and associated challenges with possible appropriate solutions.

  • Possible alternatives in crop nutrition
    109-112
    Views:
    229

    The protection of the environment is our common task. All pollution that exposes our soils, plants or the environment – as taken in any proper or extended sense – will appear sooner or later in the food chain and in human beings who are on the top of the food-chain pyramid. The aim of our work is to give a brief overview of the effects of selected industrial wastes on the physiological parameters of corn plants. Sewage sludge and lime sludge were examined. These materials contain lots of useful element for plants (e.g. iron, phosphorus, potassium, zinc). However, their aluminum, chrome and lithium contents are also considerable. The element contents in sewage sludge and the filtrates of lime sludge, as well as the dry matter accumulation and relative chlorophyll contents, were measured. The disadvantageous and advantageous physiological effects of the examined materials were confirmed. The compensation effect of the environment is excluded; however, the neutralization of environmental impacts is not infinite under natural circumstances.

  • The factors affecting the proliferation of mould fungi and mycotoxin production during the storage of wheat and the identification methods of the appearing branches
    129-133
    Views:
    273

    Nowadays, it is often suggested that, we should eat products made with whole grain cereals, despite of the fact that it raises the risk of consuming wheat products infected by mold and their toxins originated from the plough-lands and the stocks.
    Two third of the cultivated fields in Hungary are planted with cereals. The most alarming problem for food and feed security is caused by the Fusarium species. The greatest problem of all is caused by the mycotoxins. When they get into the food chain they can be a serious threat to public health. In addition, we have to face up to the problem of the effects of global warming that influence the growth of microbial infections in different ways.
    In this article we tried to summarize the effect of climate change on molds, the factors which have effect on growing and mycotoxin producing of molds and the identification methods of molds.

  • Significance of biogas production in Hungary
    127-129
    Views:
    242

    It is known that the quantity of fossil energy sources are rapidly reducing, therefore it is necessary to determine a new direction which has to point directly to renewable energy sources. Increasingly comes into view the agriculture’s energy producing nature next to it’s traditional food producing one. The enviromental protection is connected to the energy production by the ultilization of biomass for energy purposes, within the biogas production has an emphasized importance. Quasi every organic material can be used in biogas production, such as: food processing by-products, manure, sewage sludge, hoousehold waste.

  • Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
    153-157
    Views:
    501

    Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.

  • Study of some cooking and eating quality characters on some Egyptian rice genotypes
    77-82
    Views:
    311

    Some Egyptian rice genotypes [i.e. Japonica (Sakha 104), Japonica/Indica (Egyptian hybrid1) and Indica (Giza 182)] were investigated to evaluate the cooking and eating quality characters. High significant differences in grain shape were observed among rice genotypes. Hulling, milling and head rice percentage were higher in Sakha 104 than other rice genotypes, while Indica type (Giza 182) recorded the lowest values in milling and physical characters. No significant differences were found in chemical composition of the three genotypes of rice was recorded, but Giza 182 had the highest protein content. All Egyptian rice genotypes were low in gelatinization temperature and soft in gel consistency. Japonica and Indica rice varieties were low in amylase content, while Japonica/Indica rice variety was intermediate. The use of RVA is considered a good index for palatability evaluation for milled rice flour and starch. The Indica and Japonica/Indica types are low in breakdown viscosity, but higher in cooked pasta than Japonica type. Japonica type recorded the best score in panel test, followed by Indica type, while Indica/Japonica rice variety was the least accepted by Egyptian consumer.

  • Evaluation of Lipizzaner mares’ fertility data in 10 Hungarian stud farms
    23-26
    Views:
    207

    Despite numerous studies on the genetic background and performance traits of the Lipizzan breed, fertility characteristics of Hungarian Lipizzan mares have not been systematically examined. This study evaluates the reproductive performance of 220 Lipizzaner mares from 10 Hungarian stud farms, born during the period of 1981 to 2019, using breeding records from 1998 to 2022. The mares were grouped into 25 mare families and 24 birth cohorts. Data on successful pregnancies and foalings were analyzed with respect to age, family affiliation, and breeding year. Results show that mares aged 8–12 years had the highest fertility rates, while a gradual decline was observed after 15 years. Breeding activity increased markedly after 2011, peaking in 2022 with the highest number of matings and foals born. Fertility varied considerably among mare families: some achieved consistently high reproductive success, while others showed low or zero foaling rates despite repeated matings. Hungarian mare families generally produced better fertility results, whereas Fogaras families displayed greater heterogeneity. The study emphasizes the importance of monitoring reproductive efficiency in small mare families, as unfavorable fertility trends may jeopardize their long-term viability. These findings contribute to improving breeding strategies and ensuring the preservation of genetic diversity in the Hungarian Lipizzaner population.

  • The changes of the purple coneflower’s (Echinacea purpurea L.) herb and radix drug yield under different fertilization conditions
    79-83
    Views:
    355

    In this research, the purple coneflower’s (Echinacea purpurea L.) nutrient requirement was examined under different fertilization conditions in a small plot experiment. We measured the medicinal plant’s raw and dry herb and root drug mass and drying loss under different fertilization settings and meteorological factors.

    From the drug’s raw and dry mass perspective, based on the results, in our opinion, the control setting exceeded all nutrient settings’ results in 2016. In 2017 and 2018, the N75P100K150 nutrient setting has the biggest herb yield. As for the root yield, it was also the N75P100K150 setting which produced the biggest yield.

    The Pearson's correlation test was performed to investigate the connection between the quantity of the raw, the dried herbs, the different nutrient settings and meteorological factors.

  • The role of agriculture in regional development
    25-30
    Views:
    780

    Agriculture is the leading national economic sector of Hajdú-Bihar county. The proportion of employees in this sector and the share of pro ducts produced in this region is above the country average. The contribution of agriculture to the GDP is 6.3%, as opposed to the average value of the country (3.3%). Based on their primary activity, 8% of the enterprises in the county are classified as agricultural and forestry companies, while this proportion is only 5.3% in the whole country.

    Nearly three quarters (71.1%) of the sowing area is occupied by maize and other cereals, mainly wheat. This proportion is slightly more than the country average (69.2%). The proportional area of oil crops - including sunflower – and roughage is nearly the same as the country average. However, there is a significant difference in vegetable production. Due to the higher intensity of vegetable production, the area occupied by vegetables is three times the country average. 19.4% of the crop products, 23.5% of animal products and living animals, 25.8% of the income generated by production factors and 29.8% of net enterprise income of Hungary originates from the North Great Plain region.

    The facilitation of creating local markets, e.g. local public food supply, satisfying the needs of food trade with local goods, the necessary awareness raising actions, secondary and higher education of experts, as well as the more specific and targeted utilisation of development resources are agricultural development tasks.

  • Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
    81-85
    Views:
    417

    The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.

    Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.

  • Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment
    77-85
    Views:
    397

    The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the changes of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.

  • The stinging nettle as an alternative food crop
    68-70
    Views:
    194

    Vegetables play an important role in human health and have an important effect on the human diet. It is equally serious to improve the volume, the diversity and the year round equability of vegetable consumption. The stinging nettle (Urtica dioica L.) is an alternative vegetable crop and available from the beginning of Spring to the end of Autumn; therefore, it can improve both the diversity and the equability of vegetable consumption. The stinging nettle has a superb nutritive value, it is a prospinach and it can be cooked in many ways.

  • The online marketing possibilities and judgment of the domestic food-sector
    77-84
    Views:
    360

    The theme of my PhD research is the online marketing possibilities and judgment of the domestic food-sector. This article is based on the important bibliography of my research theme. I show the new categorization of the tools of marketing communication. I examine the recent years’ changes of the social approach and the interactivity. Based on the meeting of the Internet I separate our ages’ generations. Furthermore I examine the transformation of consumers into users in the digital environment. I prove the changes of the Internet’s penetration and the presence of enterprises in the Internet with statistic data. I touch the tendency of information searching in online environment. I present new methods to measure the online marketing activities’ return.

    Finally, I define my objectives of research based on my own experience and bibliography overview over and above I draw up my concrete future’s research which I separate seconder and primer section.

  • Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
    23-29
    Views:
    372

    The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.

    The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.

    This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.

  • Precision crop production and artificial intelligence – the future of sustainable agriculture
    47-58
    Views:
    628

    According to Kay et al. (2004, in Shockley et al., 2017), there are seven steps to the decision-making process: 1) Identify the problem or opportunity, 2) Identify the alternative solution, 3) Collect all data and information, 4) Analyse the alternatives and make a decision, 5) Implement the decision, 6) Monitor the results of the decision, 7) Accept responsibility for the decision. The basic question is what kind of tasks we can perform in the decision-making process and what to leave for Artificial Intelligence (AI).

  • The current status, challenges and prospects of native pig production and large-scale farming in Laos
    155-161
    Views:
    942

    This review aimed to investigate the potential native pig development and the trend that large-scale farming may influence native pig production in Laos. The review found that native pigs were estimated to be around 88% of the total pig population in Laos in 2023. Commercial pig farming was found only in the main cities, particularly the central and southern provinces, which increased from 578 in 2019 to 703 farms in 2022 and 737 farms in 2023. Due to the serious economic crisis starting in 2021, many Lao pig producers stopped their businesses, leading to the decline of the commercial pig population by almost 30% in 2023 compared to 2022. The average pork per capita consumption in Laos in 2021 was estimated at about 14.2 kg. Though not as high as the backyard, commercial farms still contribute essentially to the pork supply in Laos (12%), increasing the volume from 87,628 tonnes in 2015 to 137,775 tonnes in 2024. The main native pig producers are small-scale rural farmers who have not yet improved productivity or reproductive performance, and the main purpose of their production is household consumption rather than market orientation. However, farmers, mainly in the main cities, improved them by cross-breeding native pig sows with Duroc boar for better lean meat. The main challenges for native production in Laos include poor farm setup and management, high cost of commercial feed, and poor breeding and genetic management. Therefore, creating awareness of the benefits of setting up a pig farm and management, providing better option strategies for utilising locally available feeds, and providing better strategies on pig breeding could improve native pig production in Laos.

     

  • Competence modules of the agro-environmental engineering bachelor course in Hungary
    229-236
    Views:
    313

    One of the most important goals of the Bologna Process is to introduce the three circle system: bachelor, master, doctorate courses. In Hungary from 2006/2007 academic year 132 bachelor courses are launched. One of the launched bachelor course is environmental engineering of which the scientific background and economical sector are rapidly changing. Hence, to help graduates from this area to find a job, it is very important to examine working activities, expectations of employers, to study requirements of the training programs, the system of competencies. Having examined these points, frameworks of professional requirements have been worked out by 27 validation tests. In this publication, the results of the research work related to the environmental engineering bachelor program were presented.

  • Effects of Site on Winter Wheat Quality 2002/2003
    100-107
    Views:
    501

    The demand of modern societies for high food quality is evident. Thus, it is important for agriculture to produce row materials that are valuable for nutrition and have favourable characteristics for food processing. For this we need a knowledge about the factors which determine the quality of products. One of the main features of plant production is the “immobility”. This way the characteristics of the field influence the quality of the product, like example winter wheat, which is the main cereal in Hungary and Europe.
    The Concordia Co. has charged the Central Laboratory of Debrecen University, Agricultural Centre with laboratory testing of the 2002/2003 winter wheat crop. The samples consist of thirteen winter wheat varieties from six different sites under the same cultivating conditions. Therefore, the important wheat quality factors were analysed solely against site conditions with the use of Győri’s “Z” index, which contains these parameters.
    Soils were tested first. In this experiment excepting the negligible differences between the sites, there were no linear relations found between quality factors, productivity and soil features. The case is the same with the relation between precipitation, temperature and quality parameters. However, it must be noted that additional soil analyses are required to interpret the extreme results obtained from Karcag.
    The calculated Győri’s Z-index shows relative stability concerning certain varieties, although considerable deviation can be found in varieties related to the sites. According to these results, it can bestated that winter wheat quality was not linearly influenced by soil and weather in the 2002/2003 vegetation period. As the same cultivation technology was used in the experiment, the index was determined by genetic features. It must be noted that these findings are relevant only to this experiment.

  • Effect of feeding linseed on the fatty acid composition of milk
    45-50
    Views:
    810
    In the last decades many researches were made to change the animal product food’s composition. The production of better fat-compound milk and dairy products became a goal in the name of health conscious nutrition. These researches were motivated by the non adequate milk fat’s fatty acid composition. There have been made researches in order to modify the milk’s fatty acids’ composition to reach the expectations of functional foods. With the optimal supplement of the feed can be increased the proportion of the polyunsaturated fatty acids and can decreased the saturated fatty acids. Row fat content of milk was not decreasing in the course of examination neither of the cold extruded linseed nor the whole linseed supplement as opposed to observations experienced by other authors. In case of monounsaturated and polyunsaturated fatty acids when supplementing with cold extruded linseed the most significant change was observable in the concentration of the elaidic acid, oleic acid, linoleic acid, alfa-linolenic acid, conjugated linoleic acid. In case of saturated fatty acids the quantity of palmitic acid and myristic acid lowered considerably. When observating the feeding with whole linseed the concentration of many fatty acids from the milkfat of saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, alfa-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers as well. The producing of milk with favourable fatty acid content from human health point of view can give scope propagate the products of animal origin.

     

  • Foreword
    9-10
    Views:
    583

    The higher education of economics and agriculture in Debrecen started 150 years ago. The historical roots of our institution reach back to the middle of the 19th century. In the 1850s, the wise leaders of the city of Debrecen realised that the development of agriculture is the sole opportunity which can make the region of the Tisza River and the Trans- Tisza region the most valuable pantry of the nation. Due to the generous support of the citizens of Debrecen, as well as the enthusiastic work of scientist and teachers, the National Higher Education Institute of Farming was established on 22nd October 1868 as the first agricultural higher education institution of the Great Hungarian Plain.

  • Utilization of live feeds in fish larviculture: A review
    103-116
    Views:
    869

    This review examines the significance of live feeds in commercial aquaculture industry for production of fish and shrimp larvae, along with various challenges and prospects. Live feeds are an essential food source in larviculture, especially for marine fish, which depend on them for nutrition during the initial days of exogenous feeding. They are palatable, highly digestible, and their movement stimulates natural hunting and foraging instincts, promoting better development, lowering captivity stress, and increasing survival rates. Artemia is the most commonly used live feed in larviculture; however, it has low levels of omega-3 HUFA. Copepods are the most nutritionally rich live feed, containing high levels of DHA and EPA. Despite these benefits, they are less frequently used in larviculture due to technical challenges associated with large-scale cultivation at high densities. The use of live feed is labour-intensive and costly. The feeding period for fish larvae can be shortened using a co-feeding strategy. Microalgae rich in DHA have been utilized to enrich live feeds. Further research is needed to determine the most effective approach to significantly reduce the weaning time of fish larvae from live feed. This will lead to gradual and eventual complete replacement of life feed in the near future.

  • Biostimulant induce growth, chlorophyll content and fresh herbage yield of alfalfa (Medicago sativa L.) and variegated alfalfa (Medicago × varia Martyn) plant
    19-25
    Views:
    461

    The use of biostimulants is associated with promoting plant growth by stimulating cell division and improving nutrient availability and uptake. A study was conducted at the University of Debrecen, Hungary, to examine the effect of biostimulants on alfalfa growth, chlorophyll content and fresh herbage yield. The experiment was arranged in a randomised complete block design with three biostimulant treatments plus control replicated three times. Data collected were subjected to analyses of variance using Genstat, where significantly different means were separated at a probability of 5% using the least significant difference. The findings show no different variation in plant height or chlorophyll content (SPAD) throughout the early stages of growth. Nonetheless, a notable impact was noted in the latter stages (28 days after biostimulant treatment application) on the growth of the alfalfa plant. Biostimulant treatments did not had effect on fresh yield for second through fourth cuts, but the fifth cut showed a significant effect, with T1 treatment recording the highest herbage yield of 19745 kg ha-1 followed by T2 (Tricho Immun plus Ino Green) and T3 (Tricho Immun), with yields of 19528 kg ha-1 and 17273 kg ha-1, respectively, while the T0 (control) recorded the lowest herbage yield of 12060 kg ha-1. However, the average mean yield indicated the application of biostimulants significantly increased fresh yield herbage by 20.5%. Correlation coefficient values suggested plant height at both 14 and 28 DAH (days after harvest) strongly correlated with fresh herbage yield (r = 0.7756 and 0.7455) which reflected in the increase in fresh herbage yield. Therefore, our results suggest that the use of biostimulants in alfalfa cultivation holds promise for improving growth and yield potential through their positive effects on chlorophyll content and the growth of alfalfa plant.

  • Effect of harvesting time on the vitamin C content and yield of chili pepper
    101-104
    Views:
    389

    For thousands of years, chili pepper has been used in cooking intensifying the aroma, colouring, and even flavouring with its pungency. Today ,it is also of interest for the various food and pharmaceutical industries due to its intrinsic characteristics. These characteristics of pepper and its yield can be affected by both the variety and the temperature fluctuation throughout the harvest period. The current work's objective was to investigate the effect of harvest time on vitamin C of different varieties of chili pepper C. frutescens and C. annuum cultivated in Hungary, as well as the yield of pepper in different harvesting periods. Vitamin C was determined by HPLC protocols. The differences between harvest times and varieties were analysed using Tukey post hoc test at 5%. All varieties showed fluctuation of vitamin C level, depending on the given harvest date. At the last harvest, all varieties had higher vitamin C content. All varieties had the same yield trend throughout the year but with different yields between each. Variety Hetényi Parázs had the highest vitamin C content and yield: 3720.53 µg g-1 vitamin C and 18.63 t ha-1 average yield, respectively.

  • Nutrient Uptake of Miscanthus in vitro Cultures
    23-24
    Views:
    289

    The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
    Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
    After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days.