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A review on fish in vitro digestion studies
55-63Views:160(1) Background: In vitro experiments in fish have been developed to search for dietary substitutes of fish meal in aquafeeds by measuring the digestibility of various feedstuffs by simply simulating the conditions of the stomach or portions of the digestive tract. In vitro digestion studies involving mainly commercial fish species have been conducted to determine the digestibility of conventional and alternative ingredients in fish diets using diverse digestion models. However, there remains a significant knowledge gap, particularly regarding the enzyme functionality, digestion mechanisms in many fish species, and the factors influencing enzymatic activity. This review article is focused on the importance of the use of different enzyme sources in the in vitro digestion model to predict protein hydrolysis.;(2) Methods: For this review, a comprehensive analysis of articles was conducted to gain insights into diverse enzyme interactions and feed evaluation assessments, from the nutritional science and biotechnology sector.; (3) Results: A total of 36 peer-review papers, which include original, and review articles were selected.; (4) Conclusions: In vitro digestion studies offer a valuable tool for evaluating the digestibility of conventional and alternative feed ingredients, helping to identify sustainable dietary substitutes for fish meal while reducing dependence on live animal trials.
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Examination of feral pigeon populations at animal breeding farms
52-56Views:348In the course of our examination, we assessed biometric data that determine gender, we evaluated sexual activity, measured the nourishment and necropsies of two feral pigeon populations feeding and nesting at animal breeding farms. The changes in the body weight of the different genders were significant on both farms (p<0.05).
The body weight and the crop volume of the birds were not significantly different at the examined farms.
On both farms, the elemental food source of the birds was the fodder and corn kernels given to cattle, and was supplemented by that of the pigeons living at the Debrecen farm with food from surrounding farms and with other seeds (pea, millet, rye).
By the examination of the genitals, the birds collected from the Nyírbátor farm showed sexual activity from the beginning of spring, and in the summertime decreasing activity is characteristic, while the pigeons from the Debrecen farm are only active from the summer months. In the course of the examinations in the Veterinary Institute of Debrecen, the birds proved to be free of parasites and the results of the Newcastle disease analysis were negative as well. The bacteriologic analyses showed a different proportion of E.coli, Pasteurella multicoda, haemolysing Staphylococcus bacteria at the two cattle farms. During the examinations for the statement of Salmonellas, this causative agent was stated only in case of birds that spend nights and nested in the town, which allude to human origin. We demonstrated Eimeria oocysts during the parasitological examination of the fecal samples in 5 cases at the Debrecen, and in 1 case at the Nyírbátor farm. -
Pre- and postharvest technologies to extend the shelf life of Prunus cerasus
85-89Views:450Fresh tart cherry consumption cannot be increased without the development of an appropriate technology for its elongated storage. This requires the development and optimization of the pre- and postharvest treatments. Currently, we have only limited knowledge about tart cherry (Prunus cerasus L.) postharvest technology, however, related studies on sweet cherry (Prunus avium L.) may be adopted. In this article, we have collected the most important research results in this topic.
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Sow removal patterns in commercial breed-wean herds of Midwest, USA
9-14Views:597An observational retrospective study was done to provide up-to-date information on recent sow removal patterns from 9 breed-wean herds of Midwest USA. The study comprised of sow’s removal reasons and removal types of F1 Landrace x Yorkshire gilts entered in the herds between 1st Jan 2014 and 31st July 2016. Data was extracted from existing database on Dec 2018 and 15% of the sows were still active in the herds hence not included in the study. Descriptive statistics showed that out of the 20,009 removed sows, planned removals comprised of farrowing productivity (FP) 3,523 (17.6%) and old age (OA) 1,785 (8.9%) while unplanned removals consisted of reproductive failure (RF) 7,786 (38.9%), health problems (HP) 2,629 (13.1%), locomotion problems (LP) 1,473 (7.4%) and conformation issues (CI) 1,350 (6.8%).‘Did not conceive’ and “No heat” were observed as the main contributing factors accounting for 37.6% and 32.9% respectively for gilts & sows removed by RF.13.5% of the gilts (Parity 0) were removed from the herds before attaining their first litter of which 64.1% of their removals was due to RF. Removal type consisted of slaughter (S) 85.0%, found dead on the farm (DoF) 10.8% and euthanized (E) 4.2%. The research findings depict an upward trend of sow RF removals in the US swine herds posing a serious concern for US swine producers. Characterization and quantification of sow removals gives a revelation on the deeper intrigues about the vulnerability of the various parity in respect to common causes of RF. This helps swine producers to decisively improve on gilt replacement selection, reproductive efficiency, health and nutrition management all aimed at increasing overall swine productivity and efficiency in management. Swine farmers in the US can now focus their efforts towards curbing unnecessary RF removal within parity specifics.
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Studies on the development of food attractants catching noctuid mouths (Lepidoptera: Noctuidae)
86-91Views:397The monitoring of the most dangerous noctuid pests (Lepidoptera: Noctuidae) can be performed by species specific pheromone traps. Recently the development of traps catching female moths became the main objective of the studies. We studied the synergistic effect of vine and beer as natural additive on the effectiveness of baits containing isoamyl alcohol and acetic acid in Forró and Debrecen-Ondód. The addition of vine and beer had positive effect on catches and it was significant in case of two dangerous pest species Agrotis segetum and Lacanobia oleracea. The synergistic effect was also significant with regard to the number of detected species especially in case of lower abundances and rare species.
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Fresh fruit purchase analysis in the town’s circle in the Nothern Great Plain Region
101-106Views:237In my research I aimed to examine the previous theories about consumer habits and behaviors as well as the preference of different fruit species in the Northern Great Plain Region. Furthermore, I also wanted to know if there are any correlations between the product atributes that influence fruit consumption and different ages, genders and income categories.
The importance of my research field is reflected in the fact that issues in connection with foodstuffs, healty diet and food safety have been highly emphasized recently.
I belive in order to live longer we need to pursue healthy diet that is rich in fruits and vegetables. It seems, however, not every can follow this lifestyle in their every day diet since most people prefer junk food that contains harmful ingridients.
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An overview of swine production and marketing in Africa - Mini review
121-129Views:609Pigs are the oldest domesticated animals, though their ancestry is still uncertain because of a lack of archaeological and genetic evidence. Pigs serve a vital role in reducing the demand for livestock and livestock products in most parts of the world. Especially in the African continent, pigs serve as mobile cash implying how easily they can be converted into cash to sort pressing family needs. Pig production in Africa is different from advanced systems of production in temperate countries, however, it has similarities with most of the tropical low and middle-income nations. Pig production in Africa requires attention to enable it to contribute effectively to the growing African population. However, it has been orchestrated with a myriad of challenges including market access. This mini-review is designed in a manner to provide an overview of pig production contribution to livelihoods in Africa and associated challenges with possible appropriate solutions.
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Possible alternatives in crop nutrition
109-112Views:217The protection of the environment is our common task. All pollution that exposes our soils, plants or the environment – as taken in any proper or extended sense – will appear sooner or later in the food chain and in human beings who are on the top of the food-chain pyramid. The aim of our work is to give a brief overview of the effects of selected industrial wastes on the physiological parameters of corn plants. Sewage sludge and lime sludge were examined. These materials contain lots of useful element for plants (e.g. iron, phosphorus, potassium, zinc). However, their aluminum, chrome and lithium contents are also considerable. The element contents in sewage sludge and the filtrates of lime sludge, as well as the dry matter accumulation and relative chlorophyll contents, were measured. The disadvantageous and advantageous physiological effects of the examined materials were confirmed. The compensation effect of the environment is excluded; however, the neutralization of environmental impacts is not infinite under natural circumstances.
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The factors affecting the proliferation of mould fungi and mycotoxin production during the storage of wheat and the identification methods of the appearing branches
129-133Views:254Nowadays, it is often suggested that, we should eat products made with whole grain cereals, despite of the fact that it raises the risk of consuming wheat products infected by mold and their toxins originated from the plough-lands and the stocks.
Two third of the cultivated fields in Hungary are planted with cereals. The most alarming problem for food and feed security is caused by the Fusarium species. The greatest problem of all is caused by the mycotoxins. When they get into the food chain they can be a serious threat to public health. In addition, we have to face up to the problem of the effects of global warming that influence the growth of microbial infections in different ways.
In this article we tried to summarize the effect of climate change on molds, the factors which have effect on growing and mycotoxin producing of molds and the identification methods of molds. -
Significance of biogas production in Hungary
127-129Views:234It is known that the quantity of fossil energy sources are rapidly reducing, therefore it is necessary to determine a new direction which has to point directly to renewable energy sources. Increasingly comes into view the agriculture’s energy producing nature next to it’s traditional food producing one. The enviromental protection is connected to the energy production by the ultilization of biomass for energy purposes, within the biogas production has an emphasized importance. Quasi every organic material can be used in biogas production, such as: food processing by-products, manure, sewage sludge, hoousehold waste.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:473Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Study of some cooking and eating quality characters on some Egyptian rice genotypes
77-82Views:297Some Egyptian rice genotypes [i.e. Japonica (Sakha 104), Japonica/Indica (Egyptian hybrid1) and Indica (Giza 182)] were investigated to evaluate the cooking and eating quality characters. High significant differences in grain shape were observed among rice genotypes. Hulling, milling and head rice percentage were higher in Sakha 104 than other rice genotypes, while Indica type (Giza 182) recorded the lowest values in milling and physical characters. No significant differences were found in chemical composition of the three genotypes of rice was recorded, but Giza 182 had the highest protein content. All Egyptian rice genotypes were low in gelatinization temperature and soft in gel consistency. Japonica and Indica rice varieties were low in amylase content, while Japonica/Indica rice variety was intermediate. The use of RVA is considered a good index for palatability evaluation for milled rice flour and starch. The Indica and Japonica/Indica types are low in breakdown viscosity, but higher in cooked pasta than Japonica type. Japonica type recorded the best score in panel test, followed by Indica type, while Indica/Japonica rice variety was the least accepted by Egyptian consumer.
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Evaluation of Lipizzaner mares’ fertility data in 10 Hungarian stud farms
23-26Views:136Despite numerous studies on the genetic background and performance traits of the Lipizzan breed, fertility characteristics of Hungarian Lipizzan mares have not been systematically examined. This study evaluates the reproductive performance of 220 Lipizzaner mares from 10 Hungarian stud farms, born during the period of 1981 to 2019, using breeding records from 1998 to 2022. The mares were grouped into 25 mare families and 24 birth cohorts. Data on successful pregnancies and foalings were analyzed with respect to age, family affiliation, and breeding year. Results show that mares aged 8–12 years had the highest fertility rates, while a gradual decline was observed after 15 years. Breeding activity increased markedly after 2011, peaking in 2022 with the highest number of matings and foals born. Fertility varied considerably among mare families: some achieved consistently high reproductive success, while others showed low or zero foaling rates despite repeated matings. Hungarian mare families generally produced better fertility results, whereas Fogaras families displayed greater heterogeneity. The study emphasizes the importance of monitoring reproductive efficiency in small mare families, as unfavorable fertility trends may jeopardize their long-term viability. These findings contribute to improving breeding strategies and ensuring the preservation of genetic diversity in the Hungarian Lipizzaner population.
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The changes of the purple coneflower’s (Echinacea purpurea L.) herb and radix drug yield under different fertilization conditions
79-83Views:338In this research, the purple coneflower’s (Echinacea purpurea L.) nutrient requirement was examined under different fertilization conditions in a small plot experiment. We measured the medicinal plant’s raw and dry herb and root drug mass and drying loss under different fertilization settings and meteorological factors.
From the drug’s raw and dry mass perspective, based on the results, in our opinion, the control setting exceeded all nutrient settings’ results in 2016. In 2017 and 2018, the N75P100K150 nutrient setting has the biggest herb yield. As for the root yield, it was also the N75P100K150 setting which produced the biggest yield.
The Pearson's correlation test was performed to investigate the connection between the quantity of the raw, the dried herbs, the different nutrient settings and meteorological factors.
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The role of agriculture in regional development
25-30Views:734Agriculture is the leading national economic sector of Hajdú-Bihar county. The proportion of employees in this sector and the share of pro ducts produced in this region is above the country average. The contribution of agriculture to the GDP is 6.3%, as opposed to the average value of the country (3.3%). Based on their primary activity, 8% of the enterprises in the county are classified as agricultural and forestry companies, while this proportion is only 5.3% in the whole country.
Nearly three quarters (71.1%) of the sowing area is occupied by maize and other cereals, mainly wheat. This proportion is slightly more than the country average (69.2%). The proportional area of oil crops - including sunflower – and roughage is nearly the same as the country average. However, there is a significant difference in vegetable production. Due to the higher intensity of vegetable production, the area occupied by vegetables is three times the country average. 19.4% of the crop products, 23.5% of animal products and living animals, 25.8% of the income generated by production factors and 29.8% of net enterprise income of Hungary originates from the North Great Plain region.
The facilitation of creating local markets, e.g. local public food supply, satisfying the needs of food trade with local goods, the necessary awareness raising actions, secondary and higher education of experts, as well as the more specific and targeted utilisation of development resources are agricultural development tasks.
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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
81-85Views:387The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.
Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.
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Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment
77-85Views:364The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the changes of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.
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The stinging nettle as an alternative food crop
68-70Views:184Vegetables play an important role in human health and have an important effect on the human diet. It is equally serious to improve the volume, the diversity and the year round equability of vegetable consumption. The stinging nettle (Urtica dioica L.) is an alternative vegetable crop and available from the beginning of Spring to the end of Autumn; therefore, it can improve both the diversity and the equability of vegetable consumption. The stinging nettle has a superb nutritive value, it is a prospinach and it can be cooked in many ways.
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The online marketing possibilities and judgment of the domestic food-sector
77-84Views:351The theme of my PhD research is the online marketing possibilities and judgment of the domestic food-sector. This article is based on the important bibliography of my research theme. I show the new categorization of the tools of marketing communication. I examine the recent years’ changes of the social approach and the interactivity. Based on the meeting of the Internet I separate our ages’ generations. Furthermore I examine the transformation of consumers into users in the digital environment. I prove the changes of the Internet’s penetration and the presence of enterprises in the Internet with statistic data. I touch the tendency of information searching in online environment. I present new methods to measure the online marketing activities’ return.
Finally, I define my objectives of research based on my own experience and bibliography overview over and above I draw up my concrete future’s research which I separate seconder and primer section.
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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:358The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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Precision crop production and artificial intelligence – the future of sustainable agriculture
47-58Views:615According to Kay et al. (2004, in Shockley et al., 2017), there are seven steps to the decision-making process: 1) Identify the problem or opportunity, 2) Identify the alternative solution, 3) Collect all data and information, 4) Analyse the alternatives and make a decision, 5) Implement the decision, 6) Monitor the results of the decision, 7) Accept responsibility for the decision. The basic question is what kind of tasks we can perform in the decision-making process and what to leave for Artificial Intelligence (AI).
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The roles of mycotoxins in cereal crops production: A comparative study of Hungary and Tanzania
151-159Views:751Although Hungary and Tanzania's climatic, soil, and technological conditions differ significantly in crop production, cereals crop cultivation is of paramount importance; maize crop dominates the cultivated area (Hungary 1 million ha, Tanzania 3 million ha) both from a feed and food point of view. Unfortunately, in both countries, fungal species (Fusaria, Aspergilli, Penicillia, etc.) that produce various mycotoxins on cereals, including maize grains, are a growing concern. The situation is complicated because these fungal species and their toxins can appear not only on cereals but also on other crops. Despite the prevalence of mycotoxins in both countries, studies show higher exposure risks and contamination above tolerable levels for human consumption in Tanzania to Hungary, with Tanzania observing acute aflatoxicosis.
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The possibility of increasing at the conjugated linoleic acid content in pork
27-32Views:491The composition of fatty acids in food products is a significant factor in human health. Feeding can significantly influence the composition of fatty acids in the animal fat. We analysed the effect of feeding high CLA-content (conjugated linoleic acid) feed on the composition of fatty acids in pork. The animals were grouped according to the following: Group 1) feeding experimental, ghee-mixed feed for 76 days, Group 2) feeding the same feed, but only for 33 days, Group 3) feeding sunflower-oil-mixed feed for 76 days. Ghee contains CLA in high amount. The aim of our experiment is to analyse how the high CLA content influences the fatty acid content of pork. In the end of the fattening experiment the animals were slaughtered, then samples were taken from the loin, ham, abdomen and backfat from 10 animals from each group and analysed the fatty acid content. We found significant differences between the average fatty acid content of the samples. As an effect of feeding ghee-enriched feed, the CLA content significantly increased, compared to the control group. However, the linoleic acid and the arachidonic acid content were lower, and the proportion of fatty acids was also lower when feeding control feed.
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Site and hybrid-specific agrotechnical models in sweet corn production
105-108Views:203The effect of three agrotechnical factors (sowing time, fertilization, plant density) and two genotypes on the crop yield of sweet corn was examined on chernozem soil in the Hajdúság region in two different crop years. Compared to the 30-year average, the climate was dry and warm in 2009 and humid in 2010. The experiments were conducted at the Látókép Research Site of the University of Debrecen. In the experiments we applied two sowing times (end of April, end of May), six fertilization levels (control, N30+PK, N60+PK, N90+PK, N120+PK, N150+PK) and two crop density levels (45 thousand ha-1, 65 thousand ha-1). The hybrids we used were Jumbo and Enterprise. As regards the requirements of sweet corn production, the crop year of 2009 was dry and warm. The effect of moisture deficiency was more adverse on the crop yields with the second sowing time. On the contrary, the other examined year (2010) was significantly humid; the precipitation was 184 mm above the 30-year average and the temperature was average.
In the dry and hot crop year, the best yields were obtained with the hybrid Jumbo (25677 kg-1) at 65 thousand ha-1 plant density level on the average of the fertilization levels. The crop yields of Enterprise were also the highest at high plant density level (24444 kg ha-1). With the second sowing time the highest yields were obtained at the higher plant density level (65 thousand ha-1) with both hybrids (Jumbo 18978 kg ha-1, Enterprise 18991 kg ha-1), which confirmed the good adaptation capability of these hybrids at high plant density level. In humid crop year with early sowing time the highest yielding hybrid was Enterprise (at 45 thousand ha-1 crop density level 20757 kg-1), at the same time, Jumbo was best yielding at the higher plant density level (18781 kg-1). With the second sowing time the highest crop yield was obtained with Enterprise again (20628 kg ha-1 at 65 thousand ha-1 plant density level). With this sowing time the average yields of Jumbo, was 18914 kg ha-1 respectively. We found that dry crop year and early sowing time provided the best conditions for sweet corn production; the highest yields were obtained under these circumstances, which might be the results of the outstanding water management of chernozem soils. -
Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:436The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally.