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Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
100-104Views:355Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection. -
Susceptibility of maize hybrids to corn smut diseas
39-42Views:466Corn smut disease is one of the most common maize diseases. In the previous years the disease lost from its importance, however nowadays it is becoming more and more important and widespread. The protection against it encounters great difficulties, therefore it is important to emphasize prevention and resistance based protection. The aim of the research was to investigate under laboratory circumstances the resistance of Hungary’s most commonly cultivated maize hybrids to corn smut disease. Twelve species were investigated. We distinguished two susceptibility groups within the hybrids based on the investigated parameters. The results are intended to assist the protection against corn smut disease and to make a suggestion for the farmers so that they could apply more environmental friendly and economical production.
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Comparison of Integrated and Conventional Production of Young Nonbearing Apple Orchards
3-5Views:213The large number of pesticide applications in apple orchards creates serious problems with pesticide residues and their side effects on beneficial organisms, the environment and human health. This is the reason behind the search for new systems for apple protection.
The investigations were made in apple orchards of the Institute of Agriculture at Kyustendil, during the period from 1997-1999. Three scab resistant cultivars grafted on rootstocks MM106 were planted in 1996. The orchard was divided into four plots. Two plots were treated as „conventionally” and the other two were treated as „integrated” according to the general principles, rules and standards of integrated apple production.
The key disease during the experimental period was powdery mildew, which can be controlled only with pruning of infected clusters and shoots during the first three years after planting. The key pests in the orchard during the nonbearing period were the green apple aphid and San Jose scale. In the integrated plant protection system, it is possible to reduce the number of insecticide treatments depending on the density of the main pests. -
Dietary fibre prevalence and its role in human nutrition
9-13Views:497The role of dietary fibre and its impact on health is increasing. It is worth noting that their beneficial effects on the body are becoming more widely known, but their consumption is still below the recommended daily intake from food. Dietary fibre, as a class of compounds, includes a mixture of plant carbohydrate polymers, both oligosaccharides and polysaccharides. Dietary fibre has been linked to a number of diseases, as its consumption can reduce the risk of cardiovascular disease and type 2 diabetes. They influence the composition of the bacteria present in the human gut. Consuming the right quantity and quality of dietary fibre can play a role in the prevention of certain types of cancer. Nowadays, it is common to produce various fibre-enriched foods, called functional foods, because of the beneficial effects they have previously had on the body. In the present work we want to give a general description of dietary fibre and its role in the body.
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Examination of French bean on organic and conventional farming of Research Centre of Nyíregyháza
87-98Views:248This study presents the yield results of some French bean varieties in organic and conventional farming. This study presents the advantage of organic farming in environmental point of view and in nutrition. Sale of organic products is insured, there is solvent demand rather in abroad than in Hungary.
In Research Centre of Nyíregyháza had made organic farming since 1994, at present on 74 hectares.
In the first trial, variety comparison with 9 yellow podded French beans in organic and conventional farming was conducted. Varieties: Carson, Cherokee, Debreceni sárga, Goldmine, Héliosz, Minidor, Sonesta, Sundance és Unidor. The following parameters were observed: the time of emergence and flowering, number of plants per plot, plant height and flowing green harvest. We weighed yield of the standardized, un-standardized and diseased pod fractions. The results were evaluated statistically with SPSS and Excell softwares.
Emergence had all at once, but the plants of organic farming were 5 days earlier at flowering and maturity, than conventional farming.
The plants in organic place were more developed than in conventional ones. Emergence was more uniform, the growth and the number of plant were square. Significant difference was not detected in plant height between two places. Most of the varieties examined had better total yield in organic place, than in conventional ones. Deviation depended on variety. ‘Sonesta’ and ‘Debreceni sárga’ had the best yield in both places. In
conventional farming choice can be expanded with ‘Unidor’ and ‘Sundance’. In organic farming choice can be expanded with ‘Minidor’ and ‘Carson’.
In both places the Sonesta, Debreceni sárga and Unidor varieties had the most standardized yield per hectare. In organic place Carson variety had good pod yield because it was infected less by diseases.
In the another trial we studied inside content values of some varieties on organic and conventional places. The parameters were observed: dry matter-, starch-, crude fibre-, crude protein content and amino acid content.
The rates of asparagin or glutamine acid were the highest, which was followed by serin or histidine. In asparagin content was the most deviation between conventional and organic farming.
Significant differences were between varieties in dry matter-, starch-, crude fibre-, and crude protein content both on organic and conventional places. Each variety had significant differences between organic and conventional farming.
Starch had strong and negative correlation with dry matter, crude fibre and crude protein content.
Budai piaci and Minidor varieties and BU-16 variety candidate had higher starch content and lower dry matter, crude fibre and crude protein content on organic place. Sonesta variety had almost equal dry matter content on both places, but crude protein content was higher with 10% and starch content was lower with 6% on organic place, than on conventional place. Paridor variety had almost equal starch content, but it had higher dry matter and crude protein content on organic place. -
Inheritance of Plant and Ear Height in Maize (Zea Mays L.)
34-38Views:712Plant and ear height are very important characters not only for describing new varieties of maize (Zea mays L.), but for green and dry matter production, and even for grain yield. Significant positive correlations have been reported by various authors between plant height and stover yield, plant height and dry matter yield, and plant height and grain yield. The height of the main ear is also correlated to plant height. It depends on the variety or the environment, but is likely to be the same height within a population. Many environmental and agronomical factors (e.g. plant density, fertilization, pests and diseases) influence the expression of these characters, which are not quality traits. Their expression is controlled by many genes and by the interactions between these genes. The heritability of these traits is high and they show significant genotypic variability and positive heterosis, as reported in many research publications.
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Characteristics of roe deer bot fly infection in the southern part of the Great Hungarian Plain
57-60Views:320I am investigating the occurance of one of the diseases caused by parasites in plain roe deer populations, namely a botfly larvae, Cephenemyiastimulator, (Clark, 1815) ranged among the Oestridae family. Nasal botfly larvae are common roe deer parasites and are generally spread nationwide. The subject of the present survey is the processing of the data gained about roe deer bucks of the year 2009 and their infestation indices. I investigated botfly larvae in 90 bucks’ nasal and pharyngeal cavity. We have found botfly larva in 18.9% of the examined bucks (prevalence), this means 17 infected specimen. I collected 218 pieces of larvae from the samples, with a 12.8 pieces average intensity. In my investigations prevalence was lower and mean intensity was higher than in previous Hungarian researches. This was manifested both in my overall data, and in our examinations by the different age groups.
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The importance of selenium in the living world and in the scientific research
278-286Views:828Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Testing the antimicrobial activity of essential oils
71-74Views:288The vapor phase of some essential oils proved to have antimicrobial activity. Utilization of the vapor phase of Eos is presently understood as one of the possible alternatives to synthetic food preservatives which could be used in the future. However, testing the vapor phase of EOs against microorganisms causing food-borne diseases (e.g. Salmonella enteritidis or Staphylococcus aureus) or food spoilage is relatively new. Consequently, due to the large number of known EOs, research on their antimicrobial activity is still largely in the phase of in vitro rather than in vivo testing. Moreover, no standard and reliable method for fast screening of a wide range of samples exists. Thus, the aim of this study is to show results concerning tests of the antimicrobial activity of EOs against S. enteritidis or S. aureus, which were conducted by two modifications of the disc volatilization method we developed. The lately developed method has the potential to become widely used for fast screening of EO antimicrobial activity in the vapor phase.
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Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
188-190Views:473Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.
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Old-new disease is maize smut (Ustilago maydis)
43-46Views:621Corn smut disease (Ustilago maydis) is one of the most common maize diseases. In the previous years the disease lost some from its importance, however nowadays it turns into more and more important and widespread one. The control encounters great difficulties, therefore it should be emphasized the preventive and resistance-based protection.
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The effect of water-stress on the mineral nutrition of fruit plantations
187-192Views:429Besides agro-techniques the climatic conditions play an important role in agricultural production. Weather extremes are
significant hazards to many horticultural regions all over the word. It has a profound influence on the growth, development and yields of a
crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water
stresses. Nowadays, the weather extremes cause more and more problems and significant hazards to many horticultural regions in Hungary.
The aim of this study is to explore the problems of nutrient uptake followed from climatic anomalies and response it. In this study
we focus on water supply problems (water-stress).
Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
· Nutrient demand of trees can be supplied only under even worse conditions.
· The most effective weapon against damage of climatic anomalies is preventative action.
· Proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of
climatic anomalies.
· Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
· The role of foliar spraying, mulching and fertigation/irrigation is increasing continuously.
· Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as
modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar
fertilization is recommended, which adjusted to phonological phases of trees. Moreover, mulching is regarded as an excellent water saving
method. -
Reduction of ragweed pollution in industrial utilization system
69-72Views:270In Hungary 6.2 billion hectares are used for agricultural and from this area 5.0 billion are polluted by ragweed. In addition, the export of agricultural products will be threatened because of their pollution. Ragweed results also problems in human medicine because of its pollen allergy. More than 30% of the habitants are affected directly or indirect by allergyc diseases. On an annual basis nearly 60 billion HUF is spent on defense against ragweed. From this budget 30 billion HUF comes from the damage of the agriculture and other 30 billion for the cost of the therapy and health insurance. To solve the problems caused by ragweed needs new ideas. The proposals are the gathering and comprehensive utilization. A leap forward, from the ragweed new, market–orientated product should be developed.
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Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:862The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Preliminary study: The effect of gibberellic acid and girdling application on two dual-purpose grape varieties in a cool climate
139-144Views:617In our 2023 experiment, Nero and Muscat Pölöskei grapevines were treated with gibberellic acid (12 mg L-1) during flowering, followed by stem-end girdling at the onset of berry development. Photosynthetic activity was monitored regularly. Assimilation rate (A, µmol CO₂ m⁻² s⁻¹) and transpiration rate (E, mol H₂O m⁻² s⁻¹) were measured under natural light. Sugar content and acidity were assessed via refractometry, while berry dimensions and texture (e.g., berry hardness [Bh, mN]) were recorded. Gibberellin treatment increased sugar content compared to both control and girdled samples. However, girdling significantly enhanced sugar accumulation in Muscat Pölöskei compared to untreated samples. Gibberellin also induced berry elongation, increasing the longitudinal axis in Nero (19%) and Muscat Pölöskei (24%). Girdling temporarily reduced photosynthetic activity, which recovered post-healing. The elongated berries due to gibberellin treatment led to a looser cluster structure, which is beneficial for Nero, which is prone to fungal diseases due to dense clusters.
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The relationship between silver crucian carp (Carassius auratus gibelio bloch) and fish poulations of natural waters
30-36Views:406The fisheries management on inland waters is very complicated. Often the unclear propriety rights make the normal management difficult, and other times the stocking of the waters is problematic. In the last hundred years a lot of new fish species have been introduced to Hungary. Lot of them have found their place in the Hungarian fishfauna, till others have caused big problems for the fish farmers, and the anglers too. The biggest problem was caused by the topmouth gudgeon (Pseudorasbora parva Schlegel, 1842) brown bullhead (Ictalurus nebulosus LeSueur, 1819) and the silver crucian carp (Carassius auratus gibelio Bloch, 1782). In this article I show what kind of problems were caused by the silver crucian carp, because it seems that this fish is the most harmful.The damage caused by silver crucian carpposesa complex question. To get into the ponds and rivers, the crucian carps occupy the territory form the native fishspeciesandthesilvercrucian carp competes with these fishes for food, too.The silver crucian carphasa special reproduction method and because of this the speciesis expand rapidly. The silver crucian carp is able to reproduce with other cyprinids, and because the female silver crucian carps are triploids every fingerlings will be silver crucian carp.This fish is a vectorfora lot of diseases, which can easily spread over for other species.
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Sunflower in Romanian agriculture
153-163Views:383Romania is one of the largest producers of sunflower closely followed by Bulgaria, Hungary, France and Spain. The total sunflower production increased after 1990, due the increase of cultivated surface during the moderate yield average increasing rate. Last year, Romania was ranked first in the European Union (EU) for the area cultivated with sunflower and the total production of sunflower seeds, according to the National Statistics Institute (INS). Romania's position on the map of sunflower producers area is supported by about 1 million hectares sown. In the segment of sunflower productivity, Romania has an unfavourable position, situated in the last places in the classification of the EU's most productive agricultures. In order to increase production per hectare, it is required to perform measures aimed at the elements of sustainable cultivation technology, including the stability of the production level by ensuring phytosanitary protection.
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The impact of applying foliar fertilizers on the health condition of maize
105-109Views:433The nutrient supply of plants is becoming more important in plant protection since the appropriate condition of plants can be ensured by optimal and satisfactory nutrient supply to avoid nutritional deficiency diseases. Due to the extreme weather conditions, plants are forced to face several stress factors, which leads to deterioration of the plant health. The increasing occurrence of droughts poses threat to nutrient uptake through the roots since all the nutrients can be accessible to the plants only if they are in dissolved form in the soil – which is not possible in the absence of water. Therefore, the importance of foliar fertilizer is becoming a more and more significant part of the nutrient supply, because with the help of this technology the development of any nutrient deficiency can be prevented.
In this experiment, we focused on the efficiency of two different foliar fertilizers on maize. Foliar fertilizers were applied two times, once in the stage with 8 leaves and tasseling phenophase of the maize. To verify the efficiency of the foliar fertilizers, the chlorophyll content of untreated and treated plant’s leaves was measured after each application. Moreover, the length and diameter of maize cobs, thousand kernel weight, protein, oil and starch content were also measured, and the results were compared to the untreated (control) ones. According to the results, in all aspects significant differences were observed and due to the laboratory analysis of leaves, in the case of magnesium and zinc supply the foliar fertilizers were able to prevent the development of nutrient deficiency.
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The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency
27-32Views:597Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance.
In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.