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Impact of nitrogen and sulphur fertilization on the growth and micronutrient content of spring wheat (Triticum aestivum L.)
211-219Views:327Micronutrients are as important as macronutrients for crops. Each micronutrient has its own function in plant growth. Zinc is important for membrane integrity and phytochrome activities. Copper is an essential micronutrient required for the growth of wheat. Manganese is required for enzyme activation, in electron transport, and in disease resistance. The pot experiment was set up in greenhouse on calcareous chernozem soil Debrecen-Látókép with a spring wheat. In certain development stages (according to BBCH growth scale of wheat), at the beginning of stem elongation (29–30), at the heading (51–59), at the flowering (61–69) stage three average plants were removed from all pots for analysis. Fresh and dry weight of the plant samples were measured. Plant leaves after drying were digested by HNO3-H2O2 methods and manganese, zinc and copper contents of plant were quantified by atomic absorption spectrophotometry. At the flowering stage, when the nutrient uptake of plants is the most intensive, the weight of wheat ranged between 0.94–1.57 g plant-1. In this development stage, the NS2 treatment produced the highest weight of wheat, and compared to this the NS3 treatment decreased that value already. The results show unfavourable effect of NS3 treatment. On the basis of microelement content of wheat and the weight of a plant, nutrient uptake by plant were calculated. At the beginning of growth the starter treatment had positive effect on Cu-uptake compared to the NS1 treatment, where the same dose of fertilizer was stirred into the soil. Wheat is very sensitive to copper deficiency, so copper dissolved by starter treatment could be favourable to the early development of wheat. At flowering stage the Zn-uptake of wheat became the highest and it was between 133.7–234.6 mg plant-1. The Mn-uptake of wheat plant was higher than the Cu- and Zn-uptake of wheat.
This phenomenon can be explained by the fact that the untreated soil had higher Mn-content, than Cu- and Zn-content. To summarize the results, it can be stated, that the copper uptake of wheat was more affected by the different treatments in the stage of stem elongation, while Mn- and Zn-uptake of wheat were influenced primarily in the stage of heading and flowering.
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Advancement of a Common Wheat (Triticum aestivum L.) Selection System
8-11Views:167All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect. -
White rust species (Chromista, Peronosporomycetes, Albuginales, Albuginaceae) on common weeds in Hungary
30-33Views:423The obligate plant parasite fungi in the family Albuginaceae are responsible for causing white rust diseases on weeds and they are rather common worldwide. Weedy plants with characteristic symptoms have been collected in 2014 and 2015 on location Hajdú-Bihar and Jász-Nagykun-Szolnok counties in Hungary. The determination of the species were based on the morphological characters both pathogens and hosts. Albugo candida was determined on shepherd’s purse (Capsella bursa-pastoris). Common purslane (Portulaca oleracea) is a host for Wilsoniana portulacae. The fungus Wilsonia bliti (syn.: Albugo bliti), the causal agent of white rust disease was found on redroot pigweed (Amaranthus retroflexus).
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Preliminary test: Evaluation and selection of tomato (Lycopersicon esculentum Mill.) varieties resistant to drought and powdery mildew
33-37Views:270Powdery mildew (Leveillula taurica) and (Oidium neolycopersici) are two harmful fungi that invade the tomato (Lycopersicon esculentum Mill.) plant and grow in dry conditions. Under the influence of polyethylene glycol 6000 at a concentration of 12%, the total seedlings fresh weight, total seedlings dry weight, seedling growth rate of one seedling, seedling length, and tissue water content percentage for three tomato varieties were assessed. Despite the superiority of the (Mobil) tomato variety in terms of numerical values, the results revealed no significant differences between varieties. As a result, (Mobil) has greater vigor under environmental drought stress of lower osmotic stress than other tomato varieties. More work is required to evaluate the research selection of varieties resistant to biotic stresses in dry areas, such as powdery mildew disease.
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The Effect of Smut Gall Tumour Infection on Iron and Zinc Uptake and Distribution in Maize Seedlings
27-32Views:182The amounts of Fe, and Zn were measured in maize seedlings infected by smut gall tumour (Ustilago maydis Dc. Cda.) and in healthy seedlings five days after infection. The amount of elements was also measured under different stress intensities. Due to the infection, as a biotic stress, the amount and distribution of examined elements have been changed. On the bases of the differences in the Fe distribution between the symptoms less and tumorial leaf parts, we have come to the conclusion that the infection also effects the mobilisation of Fe and Zn inside the plants. The Fe uptake was much higher in the infected plants and the tumour development also had an effect on the uptake and distribution of the examined elements. The experiments of infecting maize seedlings by monosporidial strain of crown gall tumour showed no tumour development. We found that the monosporidial strain also acts as a biotic stress and has an effect of iron and zinc distribution. We observed a slight difference in the iron and zinc contents in the roots of corn seedlings infected by different monosporidial sporidium concentrations, while the iron and zinc contents in the shoots were increased by the intensity of the infection. The roots do not form tumours. There is no difference between the roots of the infected and healthy corn seedlings. Since the Fe and Zn contents of the shoots of infected plants depend on the intensity of the infection, we have come to the conclusion that there must exist a „special” communication system regulating the transportation of the examined elements.
In the experiments with infected maize seedlings, it became necessary to get the iron chlorosis before the disease reaches the lethal phase. Although most of the iron reserves are located in the embryo, to accelerate the chlorosis, the endosperm was removed, and it was observed, that the iron chlorosis appears later in maize seedlings when the endosperm is removed. The relative chlorophyll content of the first and second leaves was measured in iron efficient and iron deficient maize seedlings at different times.
The higher IAA content of tumorial plant tissues is already known. The treatment with IAA decreases the iron concentration in the shoots and in the roots of +Fe precultured plants and increases at -Fe precultured ones. The TIBA retards the shoot-to-root transport of IAA. When the seedlings were treated simultaneously with IAA and TIBA, higher iron concentrations were observed in the shoots and in the roots of corn seedlings.
We found extremely high iron concentrations in the roots of infected seedlings and, in line with this, serious damage to the roots was observed that this can be caused by the high iron content generated free radicals. The results demonstrate that IAA has a role in the shoot to root communication. -
Phytopatological properties of symbiotic Rhizoctonia solani strains associated to orchids
65-71Views:245The mycobiota of the Orchidarium of ELTE Botanical Garden (Budapest) has been studied applying aerobiological methods and isolating of tissue samples taken from 92 individuals of sixty orchid species. Among isolated basidiomycetaceous fungi 13 strains of Rhizoctonia solani were surviving in axenic culture. These symbiotic R. solani strains proved to be pathogenic on 24 cultivated plant species at varying degree. The symptoms of disease caused by R. solani strains isolated from orchids did not differ from that caused by reference strains. Three groups of strains could be separated regardless of their source or aggressivity. The host plants clustered into two groups, and their taxonomic position had no role in this respect. In general, we can assume that orchid associated Rhizoctonia strains are potential plant pathogens, and removed or withdrawn orchid stools should be treated as hazardous waste.
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Examination of the presence and effect of udder edema in Holstein-Friesian cattle
125-130Views:289Mastitis is the most common disease of dairy cows and the most expensive in terms of treatment. For this reason, great emphasis must be placed on its prevention. Based on literature sources, it can be said that there is a positive correlation between the prevalence of udder edema and the occurrence of mastitis. Our studies were carried out in a dairy farm in Csongrád-Csanád county. So far we have collected data from 64 cows with udder edema. The severity of udder edema was evaluated on a scale of 0–3 points one week before calving and for 3 weeks after calving. We observed that udder edema occurred in 78% of the studied cows at the time of the first calving. After calving, the length and diameter of the four udder teats were examined. Both anterior and posterior teats were shortened in severely edematous cows. When examining the diameter of the teat, we found that, depending on the severity of the edema, the anterior and posterior udder teats were 2.5 and 1.6 and 1.6 and 1.5 mm thicker respectively than those of cows without edema.
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Laboratory diagnoses of the isolates of chestnut blight disease fungus Cryphonectria parasitica (MURR. BARR)
45-52Views:267Chryphonectria parasitica, the casual agent of chestnut blight, is one of the most important fungal pathogens of chestnut (Castanea spp.) in Europe and Hungary. In this study, we analyzed the ITS region of five Cryphonectria parasitica strains isolated from different location of Hungary. The differences among the Cryphonectria parasitica isolates were not insignificant because only two sites were considered as informative for the parsimony analysis. As the differences among geographically different isolates were insignificant, we mean that the evolutionary distance by ITS sequences within Hungarian Cryphonectria parasitica isolates is too small to get well based consequences for the phylogenetic relationships.
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Biological control of sweet chestnut on Pécsbánya, Hungary
77-81Views:391The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
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Testing disease resistance in autumn wheat genotypes by means of field experiments
30-40Views:280According to our scientific results we can state that we have to use integrated pesticides management in crop protection against the diseases of winter wheat. One of the most important elements of IPM is to select a genotype characterised by good resistance to diseases (and by high yield ability and excellent baking quality). It is especially important that the wheat variety have tolerance against not only to one or two leaf and spike (grain) diseases, but „complex” tolerance. It is not necessary to give up the growing of a variety which has susceptibility to different diseases because we can protect it using appropriate chemical management. In the intensive growing stage of wheat (BBCH 32-37) we can use a noncompulsary fungicide-treatment (depending on e. g. the infection, ecological conditions) and, at the beginning of the flowering stage
(BBCH 59-65), we have to use a compulsary fungicide-treatment (in spite of e. g. special weather conditions, resistance genotype)to ensure high yield and good quality. -
Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
47-50Views:318In the last 20–30 years lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimising lameness. Those areas are related to poor facilities, lack of straw, problems with labour and basic management.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:402The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
25-29Views:234In the last 20-30 years, lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimizing lameness. Those areas are related to poor facilities, lack of straw, problems with labor and basic management.
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Analysis of the plant physiological effects of late, artificial corn smut infestation using remote sensing methods
31-35Views:381In Hungary, corn is also infected by several important pathogens. In this experiment, we analysed the plant physiological effects of artificial late cron smut infestation using remote sensing methods under field conditions We examined the experimental area from which the data comes from with a DJI Phantom 4 Multispectral Drone NDVI and NDRE indices were calculated and analyzed in GIS programs. Individuals treated with a higher dose remained much greener than the untreated control. They also showed significant differences within the indices used.
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Susceptibility of stem infected sweet corn hybrids to common smut disease
55-57Views:795The common smut of maize (corn smut, Ustilago maydis /DC/ Corda) can cause large economic losses in susceptible sweet corn hybrids as well. The protection against this pathogen is fundamentally based on prevention. Many methods to control corn smut have been recommended or evaluated, including crop rotation, sanitation, seed treatments, modification of fertility, and biological control. In spite of these frequently mentioned control strategies, the host resistance seems to be the only effective method to manage common smut in those areas where Ustilago maydis is prevalent.
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Food allergy, safety and analytics
323-326Views:168The food allergy is a hypersensitivity reaction against naturally occurring proteins in food. These types of disease can cause not only personal inconvenience to the patient but serious health, food safety and food analysis, social-economic problems. The only effective treatment for these illnesses is a life-long diet avoiding the allergenic foods or components of food. In the interest of the patients’ health 14 allergenic components must be labeled on the food packaging. To meet the requirement of regulation reliable and valid analytical methods are necessary which for the most allergenic foods are not available.
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The role of type traits of dairy cattles in long productive life
11-18Views:275In the last few decades, a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Additionally, the reproduction biology of the population was adversely affected by keeping and feeding technologies. Productive lifetime has shortened and the service period has lengthened. However, profitable milk production requires cows with longer productive lives and larger life-time productions. In our study, we made a comparison between production, culling causes, and type traits of culled Holstein Friesian cows after first calving and of cows with a minimum of eight lactations. We established that the first lactation production of cows with longer productive lives was more or less homogeneous. Disease of metabolism as well as digestive and respiratory problems, were the main causes of the culling of cows with short productive lives, and reproduction and udder problems as well as low production, were the main causes of the culling of cows with long productive lives. We found a significant difference in the distributions of strength, body depth, dairy form, croup width, rear leg side view, foot angle, fore udder attachment and teat placement between the two groups. Furthermore, we established that cows with lower stature, less strength, a somewhat deeper body, better dairy form, narrower croup width, smaller foot angle, looser fore udder attachment and some outside teat placement had longer productive lives.
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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:522Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Flesh firmness examination of scab-resistant apple varieties in a storage experiment
93-98Views:258Of the different physical characteristics of fruits, one of the most essential qualities is flesh firmness, as is an important parameter when selling fresh apples, processing the fruit (processing industry) and during its storage. In the scope of our research, we examined five apple varieties originating from the Derecske horticultural site of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The five examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The two-month storage experiment was conducted at 16-17 °C, which had an aggressive effect on our stored apples. Flesh firmness, weight and diameter were measured on three dates for each of the five apple varieties. Our results were evaluated using IBM SPSS Statistics 20 software. We aimed to compare flesh firmness, weight and diameter of the five apple varieties and their changes during storage. Isaaq (86.34 N/cm2), Modí (94.06 N/cm2) and Fujion (84.90 N/cm2) had outstanding initial flesh firmness results. The Modí apple variety provided the best storage parameters.
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An unusual occurrence of Taphrina deformans on apricot trees in Hungary
86-92Views:206In 2011 a very rare occurence of leaf curl (leaf blaster) disease symptoms of apricot (pathogen: Taphrina deformans) was observed in different apricot plantations in Eastern-Hungary (Debrecen-Józsa, Hajdú-Bihar county) and Northern-Hungary (Bekecs district, Borsod-Abaúj-Zemplén county). The pathogen is common on peach and nectarine resulting serious symptomps and considerable damages in almost every year, although in this year infections of peaches were very low close to the locations. Although there are some references that apricot and even almond are hosts of the fungus but in Hungarian circumstances the infestation on apricot counts unique and rare. Observations were made on symptoms, microscopic photos and measures were taken on exoasci, ascospores and blastospores, respectively. As far as we know this is the first description of Taphrina deformans fungus on apricot in Hungary.
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Distribution and damage of bacterial canker on lemon bushes in the conditions of the Southern part of Azerbaijan
41-49Views:196This paper summarises and presents the results of two-year (2020–2021) field and laboratory studies on the spread and harmfulness of bacterial canker of citrus crops (Xanthomonas citri (Hasse) Dowson.) on mid-ripening lemon cultivars (Citrus limon L.). Experimental field research conducted by us in 2020–2021. It has been established that bacterial canker (Xanthomonas citri) is found mainly on lemon bushes (Citrus limon L.). The pathogen also causes canker (Xanthomonas citri) on kinkans (Fortunella margarita (Lour) Swingle.), poncirus (Poncirus (L.) Raf.), lime (Cirtus aurantiifolia (Christm.) Swingle.). Japanese cultivars of oranges (Citrus sinensis (L.) Osbeck.) and citron (Citrus medica L.) are distinguished by significant tolerance. The most resistant are tangerines (Cirtus reticulata Blanco.) – they are almost not affected by bacterial canker (Xanthomonas citri).
We have established environmental factors that contribute to the wide spread and development of bacterial canker (Xanthomonas citri) on lemon bushes in the southern part of Azerbaijan.
The main indicators of the harmfulness of bacterial canker (Xanthomonas citri) have been identified. The potential for the death of canker-infected lemon fruits has been established. The characteristic features of external and internal signs of the disease were established. A scheme for combating bacterial canker (Xanthomonas citri) has been developed, and the optimal concentration of fungicides that retard the growth and development of pathogens has also been established. Photographs of bacterial canker (Xanthomonas citri) damage to lemon fruits are presented.
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Inland and foreign Erwinia amylovora isolates by carbohydrate utilization
29-33Views:135Fire blight, a plant disease caused by the bacterium Erwinia amylovora, produces serious losses in apple and pear orchards all over the world. Since the appearance of fire blight in Hungary (Hevesi, 1996) Erwinia amylovora isolates were collected in different years, from different hosts and areas in order to establish gene bank for future epidemiological studies. We had isolates from foreign countries as well. The aim of our research was to compare all of the Hungarian and foreign isolates by carbohydrate utilization.
In our experiments effect of carbohydrates on E. amylovora multiplication was determined using API 50 CH strip (bioMérieux, France). By the API 50 CH strip method we checked a number of unstudied carbohydrates. The results of the tests shows colour changes. Based on utilization of 49 carbohydrates of API 50 CH kit by E. amylovora isolates, two groups of carbohydrates can be defined: “Utilized” - and “Not utilized” carbohydrates. All isolates utilized 20 different carbohydrates after 164 hour incubation. Conversely, isolates also could be divided into four groups (1, 2, 3, 4) by arbutin and raffinose utilization. In group 1.-isolates utilize arbutin; 2.- utilize raffinose; 3.- utilize both arbutin and raffinose; 4. -utilize neither arbutin nor raffinose. Presumably carbohydrate content of nectar could play an important role on invasion of the (E. amylovora) bacterium via flower.
It could be concluded that the carbohydrate utilization – completed with genetic analysis – can be used for characterization of Erwinia amylovora isolates. -
The Effect of Plant Density on the Yield of Sunflower Hybrid’s in 2001-2002
184-189Views:412Nowadays, for increasing efficiency of sunflower production treating hybrid-specific technologies was required. Increasing of hybrid choice gave reasons for trials in respects of critical factors, as well as in case of genotype-enviroment interactions. The effect of changing plant density show up as determinant factor which affects on yield as well as on plant hygienic conditions. Trials were established on calcareous chernozem soil (Hajdú-Bihar county), in 2001-2002. The field trials were randomized, in four repetition on small parcels. The plant density trials were established in 35.000-75.000 plant/hectar interval using a scale of 10.000 plant/hectar. 10 hybrids were used in both year.
In 2001, 55.000 plant/hectar density was the optimal, in case of most of the hybrids. The yield of tested hybrids did not show significant difference. Yield decreasing effect of using less than optimal density was more significant than in case of using optimal plant density. In 2002, the optimal density was in 45.000-65.000 plant/hectar interval, there was no possibility to find narrower optimum.
Presence of Diaporthe helianthi and the damage caused by the pathogen was significant in 1997-1999, whereas in 2001-2002 the large-scale appearance of the pathogen did not occur. In 2001, the affect of high plant density on disease caused by Diaporthe helianthi showed just tendency-like appearance, in 2002, the infection showed up just in the end of the vegetation period, without significant damage. In spite of the low infection level, the difference between the infection of the diverse plant density treatment was significant. Increasing plant density made increase the frequency of the Diaporthe helianthi infection. The rate of the flower diseases was around 3-16% in 2001, the highest infection level emerged in 65.000-75.000 plant/hectar density, whereas in 2002, the rate of infection level was less than 10%. The increasing plant density helped the development of flower diseases. -
The potential use of Pediococcus spp. probiotic in aquaculture: A review
99-106Views:475Aquaculture production has significantly increased over the previous few decades. However, antibiotics have been implemented regularly and extensively to overcome outbreaks of pathogens in aquaculture to cover the human needs for animal protein, leading to the appearance of resistant strains that may cause serious damages in the environment and also human health. In the last few years, the implementation of probiotics as an alternative technique to antibiotics use in fish production has achieved promising results in aquaculture due to their beneficial impact on fish health and growth performance. Among different types of probiotics, the Pediococcus spp. bacteria stand out as a promising probiotic for their beneficial impact to aquaculture. Thus, the current study has been conducted to give an overview about the interactions between Pediococcus spp. and aquaculture. In addition, this review highlights the role of Pediococcus spp in promoting growth performance, improving feed conversion ratios and the intestinal architecture, enhancing the immune response and inhibiting fish pathogens, thereby preventing or at least reducing the use of antibiotics. Practical use of Pediococcus spp. probiotic in aquaculture as feed additives through selected case studies is also considered.
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Examination of feral pigeon populations at animal breeding farms
52-56Views:228In the course of our examination, we assessed biometric data that determine gender, we evaluated sexual activity, measured the nourishment and necropsies of two feral pigeon populations feeding and nesting at animal breeding farms. The changes in the body weight of the different genders were significant on both farms (p<0.05).
The body weight and the crop volume of the birds were not significantly different at the examined farms.
On both farms, the elemental food source of the birds was the fodder and corn kernels given to cattle, and was supplemented by that of the pigeons living at the Debrecen farm with food from surrounding farms and with other seeds (pea, millet, rye).
By the examination of the genitals, the birds collected from the Nyírbátor farm showed sexual activity from the beginning of spring, and in the summertime decreasing activity is characteristic, while the pigeons from the Debrecen farm are only active from the summer months. In the course of the examinations in the Veterinary Institute of Debrecen, the birds proved to be free of parasites and the results of the Newcastle disease analysis were negative as well. The bacteriologic analyses showed a different proportion of E.coli, Pasteurella multicoda, haemolysing Staphylococcus bacteria at the two cattle farms. During the examinations for the statement of Salmonellas, this causative agent was stated only in case of birds that spend nights and nested in the town, which allude to human origin. We demonstrated Eimeria oocysts during the parasitological examination of the fecal samples in 5 cases at the Debrecen, and in 1 case at the Nyírbátor farm.