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Data on the Orthoptera fauna of characteristic agricultural landscape in the Carpathian Lowland
25-34Views:145Orthoptera fauna and assemblages of natural and semi-natural grasslands of the Hungarian Lowland are well-known, however, little is known about assemblages living in agricultural and anthropogenic habitats such as arable lands, roadsides, hedges, and riverbanks. Due to climate change, intensification of agriculture, and change of habitat use, these habitat types become increasingly important.
To collect data on these mainly unknown habitat types, a three-year study was carried out on the Orthoptera fauna and assemblages of the firth region of the Tisza and Sajó rivers. This area was mainly unknown, and our research contributes to increasing knowledge and provides a base for further investigations.
In the 40 sampling sites of the studied region, an occurrence of 30 Orthoptera species was recorded based on 2241 sampled individuals. In this study, we provide 540 new distribution data records of orthopterans that means an almost eightfold increase of the known data. Orthoptera assemblages of different agricultural habitat types showed significant differences considering both species richness and composition. Data suggested that non-cultivated habitat patches of dirt-roads, roadsides and stubble fields and even extensively used pastures, hayfields and alfalfa, red clover, and even wheat fields can preserve relatively species-rich Orthoptera assemblages. Contrary weedy sites of these cultivars and intensively used arable lands (maize, sunflower and rape fields) showed extremely low species diversity.
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A forgotten sour cherry pest, the stone fruit weevil (Anthonomus /Furcipes/ rectirostis L.) appeared again
104-106Views:69The stone fruit weewil (Anthonomus rectirostris L.) has been known as the kernel pest of the wild cherry in Hungary. There have been no data about its harm on sour cherry (Prunus cerasus L.) in our country, yet. 5-10% of stone infection has been observed on some sour cherry trees (cultivars: Debreceni bőtermő, Újfehértói fürtös) in the eastern side of an orchard at Debrecen-Józsa adjacent to a wood in early July 2011. The damage can be in connection with the fact that the yield has not been harvested for years.
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Texture analysis as a method for grape berry characterization
Views:169The marketability of table grapes is mainly characterized by berry size, color, taste and texture. Mechanical measurements of table grape berries could provide objective information on the textural qualities of grape berries. In addition, this method might be suitable to study the effects of phytotechnical methods (such as girdling) on table grape quality. The aim of this preliminary work was to demonstrate how instrumental testing could be used to examine the effects of girdling on berry grape texture and define the textural characteristics of table grape berries. Cane girdling was carried out at veraison in two table grape varieties. Texture analysis was performed several times during the maturity. Besides this examination another five varieties were analyzed to assess their berry mechanical properties. Double compression test was used to determine berry hardness and its derived parameters. Puncture test was applied to evaluate skin hardness, skin elasticity and skin break energy. Skin thickness was also investigated. Berry hardness, skin hardness and skin thickness of the girdled grapevines were significantly affected by this technique. Most of the textural parameters showed differences among the seven cultivars.
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SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
17-24Views:265The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers. -
Contributions to the 2014 and 2015 flight pattern and damages of Carpomyia schineri Loew.
96-99Views:126The fly Carpomyia schineri Loew is a pest of the berries of the feral Rosa canina group but is can also be found in the berries of grown rose cultivars. The larva damages the flesh of the berry, several larvae can develop inside a single berry. It can decrease the quantity and ascorbic acid content of the tea and jam, which are important human ascorbic acid sources during winter. At the same time in trapping experiments the adult can be confounded with adults of the walnut husk fly (Rhagoletis completa Cresson). In collections of berries at several sites in Hungary damage levels ranged from 0.88% to 65.08 %. Based on these damage levels we had the impression that the yellow sticky traps CSALOMON® PALz or PALs baited with the synthetic Rhagoletis lure caught moderate numbers of adults and were not satisfactory for detection, consequently improving trapping methods is necessary in the future.
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The effect of water-stress on the mineral nutrition of fruit plantations
187-192Views:84Besides agro-techniques the climatic conditions play an important role in agricultural production. Weather extremes are
significant hazards to many horticultural regions all over the word. It has a profound influence on the growth, development and yields of a
crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water
stresses. Nowadays, the weather extremes cause more and more problems and significant hazards to many horticultural regions in Hungary.
The aim of this study is to explore the problems of nutrient uptake followed from climatic anomalies and response it. In this study
we focus on water supply problems (water-stress).
Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
· Nutrient demand of trees can be supplied only under even worse conditions.
· The most effective weapon against damage of climatic anomalies is preventative action.
· Proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of
climatic anomalies.
· Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
· The role of foliar spraying, mulching and fertigation/irrigation is increasing continuously.
· Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as
modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar
fertilization is recommended, which adjusted to phonological phases of trees. Moreover, mulching is regarded as an excellent water saving
method. -
Sequence heterogeneity of nSSR and cpDNA loci of Cucurbits (Citrullus sp.)
125-134Views:79The evolution of water melon (Citrullus lanatus) microsatellites from the 15th century (Debrecen); 13th (Buda); and 18th century, (Pannonhalma) were analyzed. Microsatellite (nSSR, nuclear simple sequence repeat) and cpDNA profiles of the aDNA (ancient DNA) of seed remains were compared to modern water melon cultivars and landraces. Sixteen primer pairs were applied. Sequence analysis at the (CT)26 and cpDNA trnV loci revealed a (CT)3 and Adenin deletions, respectively, form the current water melon cultivar compared to the medieval sample. Cila-1), a new LTR retrotansposon has been described. For morphological reconstruction, a dendrogram produced by SPSS11 based on the presence versus absence of 24 phenotypic characters were also analyzed.
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Promising Leuce poplar clones in sandy ridges between the rivers Danube and Tisza in Hungary: a case study
111-113Views:214An intensive integrated research and development work has been carried out on the improvement of Leuce poplars including primarily the native white poplar (Populus alba L.) and its natural hybrid grey poplar (Populus × canescens). More than 70 percent of the Leuce poplar stands can be found on calcareous sandy sites in the Danube–Tisza region, so they play a significant role in the poplar management of this part of the country. The most important task ahead of Hungarian poplar growers is to improve the quality of poplar stands and plantations based on selecting new clones and cultivars. The growth and yield of four promising Leuce poplar clones was evaluated on a marginal site in central Hungary. The clones ‘H 425-4’ (Populus alba L. × Populus alba L.), and ‘H 758’ (Populus alba L. Mosonmagyaróvár 124) seem to be suitable for wood production, while the ‘H 427-3’ (Populus alba L. × Populus alba L. cv. Bolleana) and ‘H 422-9’ (Populus alba L. × Populus grandidentata (Michx)) clones (with decorative stem form) could be better used for tree lines and ornamental plantations.
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Preliminary studies to evaluate the use of spectral data in monitoring of apple orchard parameters
37-41Views:92The introduction/application of precision agricultural technologies has more important role in various fruit growing sectors among others apple growing. Remote sensing methods can detect electromagnetic waves where the green colour of the leaf is responsible for the chlorophyll content. The absorption of chlorophyll is in the wavelength range of 450–670 nm. Samples of apple tree leaves were taken on a weekly basis from the apple orchard at Horticultural Unit of Pallag on University of Debrecen in 2019 summer. Our studies were performed on 2 cultivars (Early Gold, Golden Reinders) and the samples were processed using 2 methodologies: a non-destructive spectral method and spectrophotometric method chlorophyll and carotenoid contents were calculated, which were created into some groups and compared with the spectral values. When the plant begins to lose strong green colour and turns yellow spectral measurements show that chlorophyll content decreases as the proportion of chlorophyll-carotenoid in the plant changes. In case of grouping into intervals, it can be observed that as the chlorophyll content increases the reflectance value decreases continuously due to the strong absorption. Based on the results, close relationship between the pigments can be detected.
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Effects of water deficit on the growth and yield formation of maize (Zea mays L.)
143-148Views:148Maize (Zea mays L.) is the most important consuming cereal crop in the world after rice and wheat. This requires an understanding of various management practices as well as conditions that affect maize crop performance. Water deficit stress during crop production is one of the most serious threats to crop production in most parts of the world and drought stress or water deficit is an inevitable and recurring feature of global agriculture and it is against this background that field study of crops response to water deficit is very important to crop producer and researchers to maximize yield and improve crop production in this era of unpredicted climatic changes the world over.
A pot experiment was carried out to determine the effects of water deficit on growth and yield formation of maize. Two maize cultivars were used Xundan20 and Zhongdan5485. Three levels of soil water content were used in two stages of water control levels at two stages of the maize plant development
1. The JOINTING STAGE: A. CONTROL (CK) soil water content: from 70% to 80% of soil water holding capacity at the field, soil water content: from 55% to 65% of soil water holding capacity at the field, soil water content: from 40% to 50% of the Soil water holding capacity at the field.
2. The BIG FLARE PERIOD: A. CONTROL (CK) soil water content: from 75% to 85% of soil water holding capacity at the field, soil water content: from 58% to 68% of soil water holding capacity at the field, soil water content: from 45% to 55% of the soil water holding capacity at the field.
This research mainly studied the effects of water deficit on physiological, morphology and the agronomical characteristics of the maize plant at the different water stress levels.
The importance of these results in this experiment will enable plant producers to focus and have a fair idea as to which stage of the maize plant’s development that much attention must be given to in terms of water supply. -
Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:86Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Effect of Storage on Fruitquality of Apricot (Prunus Armeniaca)
164-169Views:96The quality of a product is determined by numerous characteristics. As quality characteristics are polygenic, they cannot be improved easily. Moreover, there often is a negative correlation between the different parameters in the case of apricot. The firmness of fruits decreases with increasing size. The year effect also has a great influence on the expression of the potential quality of the given cultivar.
Our examinations were carried out in 2004 at Boldogkőváralja, in the cold storage plant of the Northcot Ltd. We monitored the development of fruits from fructification until harvest, and also the quality changes during storage. 100 fruits were observed from each cultivar directly after harvest. We measured height, width, weight, firmness, dry matter content and seed weight. From each cultivar, 100 fruits were stored, of which 25 fruits were observed each week. The value and the dynamics of the changes in the parameters compared to the values at harvest varied greatly for the different cultivars. Strong correlations between the parameters have not been found within one cultivar either. The heavier fruits did not lose more weight, either in absolute value or relatively, than the lighter fruits. The increase in the firmness of fruits was not accompanied with a desirable decrease in the dry matter content. -
SNP analysis of ITS1-5.8S-ITS2 rDNA loci in modern and ancient melons (Cucumis melo)
120-124Views:89ITS (internal tanscribed spacer) profiles of the aDNA (ancient DNA) of seed remains extracted from an extinct sample recovered from the 15th century (Budapest, Hungary) were compared to 31 modern melon cultivars and landraces. An aseptic incubation followed by ITS analysis was used to exclude the exogenously and endogenously contaminated (Aspergillus) medieval seeds and to detect SNPs in ITS1-5.8S-ITS2 region of rDNA (ribosomal DNA). SNPs were observed at the 94–95 bp (GC to either RC, RS or AG) of ITS1; and at 414 bp (A-to-T substitution), 470 bp (T to Y or C), 610 bp (A to R or G) of ITS2. The results facilitate the final aim of molecular and morphological reconstructions of ancient melon tpyes.
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Effect of Ozone Exposure on Phytopathogenic Microorganisms on Stored Apples
9-13Views:93The aim of our study was to clarify the effect of ozone exposure on several phytopathogenic fungi on stored apple fruits under different storage conditions. The study was conducted at Bistrita, Romania, in the storehouse of an experimental apple orchard in 2002 and 2003. Two widely grown apple cultivars (‘Jonathan’ and ‘Golden Delicious’) were used. General microbial examination of the fruits was made during storage in order to identify the most important storage pathogens. Efficacy of six ozone treatments was evaluted on fruit decay caused by phytopathogenic fungi. Monthly observations (January, February, March and April) were made of the degree of decay and three measurements were assessed (disease frequency, disease intensity and degree of attack). Our results showed that the most important phytopathogenic fungi during storage was blue mold, caused by species of Penicillium. Disease frequency of apple fruits was very high on cv. ‘Jonathan’, much higher than on cv. ‘Golden delicious’. Ozone treatments (25 ppm ozone for 0.5 and 1.5 hours in November) caused significantly lower disease incidence on stored apple than all other ozone treatments. For longer storage, it seems that additional ozone treatments in February increased treatment efficacy. Cv. ‘Golden delicious’ seemed to be more resistant to storage diseases than cv. ‘Jonathan’ both on the untreated and treated fruits. The effect of the ozone treatments was also the most effective when 25 ppm ozone was applied for 0.5 and 1.5 hours in November.
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The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
39-44Views:133Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.
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Evaluation of the Green bean variety candidate BU-16
75-81Views:72This study presents the results of the variety comparison trials conducted with the French bean variety candidate BU-16 on sandy soil, during two years at the Research Center of the University of Debrecen.
The outstanding characteristics of this variety candidate are the early maturity, the showy pods (yellow, straight, cylindrical cross-section „pencil bean”) and the high yield potential.
The experiments were conducted with a randomized design, with five varieties and four replications. The varieties (of which two are foreign and three are domestic) were as follows: BU-16 fj, Maxidor, Paridor, Hungold and Budai piaci. The individual plots were 2 m wide and 4 m long. The seeds were sown at a density of 30 germs per m2, with a row spacing of 50 cm.
The following parameters were taken: time of flowering, time of green maturity, plant height, height of the lowest pod above ground level, number of pods per plant, distribution of the pods by the state of development (20 plants), usable pod length (1 kg of standard crop-yield), yield per hectare based on the number of plants per plot. The experiments were evaluated statistically with Excel and SPSS softwares.
Results obtained with the variety candidate BU-16:
– The time from the emergence to green maturity is 45 to 46 days, which is short as compared with the other cultivars studied.
– Plant height is 38cm (two years average), which is among the highest ones, together with Paridor.
– The pods are set high above the ground level similarly to Paridor, the height of the lowest pod is 19 cm.
– The average number of pods per plant is 13 (two years average) similarly to Paridor and to Maxidor.
– The distribution of pod size in 2007 is as follows: 68% of the pods of BU-16 is of the standars size, which is a good rate. The value of this trait for the other varieties is 60 to 63%, except for Paridor, the value of which is similar to that of BU-16.
– The usable pod length (10 to 14 cm) is 63% in 2007 and 84% in 2006. Similar pod length rates were obtained for Maxidor, while the rate for Paridor is as high as 91%.
– The total yield and the standard yield of BU-16 is among the highest ones.
According to the results obtained and presented above, the variety candidate BU-16 is the earliest maturing among the varieties tested with high yield potential which, however, is not significantly different from that of the others. Significant differences can only be detected in plant height and the number of pods per plant. Considering the results, BU-16 is to be further tested. -
Correlations Between the Crown Sizes, Pruning Times and Fruit Quality of Sour Cherry Cultivars
90-94Views:156To increase the intensiveness of sour cherry production, i.e. the use of smaller trees, it is necessary to adopt growth-moderating techniques rather than using dwarfing rootstocks. Apart from the traditional technique of using rootstocks that support stronger growth, new or rarely-used techniques and methods must be adopted. Pruning should be carried out in the summer rather than in the winter, the optimal period being 1-3 weeks after harvest.
A combination of the increase in favourable exposure-time and smaller crown sizes make higher tree densities (tree/ha) and better utilisation of the crown (specific cropload) possible.
The systematic use of summer pruning leads to better quality fruit. -
The significance of sour cherry in iron supplementation
101-104Views:144The iron concentration of ’Csengődi csokros’, ’Debreceni- and ’Érdi bőtermő’ , ‘Éva’, ’Kántorjánosi’ ,’Petri’ and the ’Újfehértói fürtös’ cultivars was determined by ICP-MS. Furthermore the Vitamin C, L-Malis acid and Citric acid concentration of samples were measured.
Our results show that large amount Fe2+ (average 20.5 mg kg-1) accumulates in the pulp of sour cherry. Besides, the concentration of Vitamin C, L-Malis acid, that increase the absorption of iron, are high. Based on these results, the sour cherry and the products of sour cherry play an important role in the forming of iron content in body.