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Assessment and comparison of selenium-enriched maize with sodium selenite and sodium selenate
11-15Views:176Selenium is an element of environmental interest owing to the narrow range between its nutritionally required and toxic concentrations in many organisms. Its mobility and bioavailability differ greatly depending on individual Se species. In this regard, in present study, the uptake and distribution of Se, the changes in Se content, and the effects of different concentration of Se in two forms of sodium selenite and sodium selenate on maize plants were measured in nutrient solution experiments to clarify their response to the two forms of Se. The results revealed that the Se content in shoots and roots of maize plants significantly increased as the Se level increased. Two Se forms behaved differently and the effects of toxic damage in samples which had been treated with selenite were much more than in the selenate treatments.
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The study of the fertilizing effect of wheat straw ash in a greenhouse experiment
47-51Views:186The effect of wheat straw ash as a fertlizizer was studied in a pot experiment with an acidic sandy loam soil (pHKCl=4.9) with weak K and P supply. The test plant was ryegrass (Lolium perenne). The treatments were the following: 1. control untreated soil, 2. NPK fertilizer, 3. small dose of ash (1.4 g kg-1), 4 large dose of ash (2.8 g kg-1), 5. small dose ash completed with NP fertilizers. Soil parameters (pHH2O, pHKCl, ammoinum-lactate soluble P, K, 0.01 M CaCl2 soluble PO43--P, K, Mn, Cu, Zn ) and plant parameters (yield, P, K, Ca, Mg, Zn, Mn uptake) were investigated. Based on the analysis of the straw ash sample and the results of pot experiment it can be stated that the wheat straw ash is suitable for the fertilization of the studied soil. The small dose ash completed with NP resulted in the largest yield increment (43%). In order of the treatments the pHKCl changes to: 4.9, 4.8, 5.2, 5.8, 5.1. As the N : P2O5 : K2O ratio is 0 : 1: 3.5 in the wheat straw ash sample, to reach optimal yield ash should be completed with N and P.
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Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
17-20Views:267The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.
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Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles
33-39Views:169Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the organic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.
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Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
23-28Views:324A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.
On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.
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Testing laboratory parameters of compost tea
31-36Views:430During the industrial production of broiler chicken, a large amount of manure is produced, of which easily contained nitrogen content (without pre-treatment) is released into the atmosphere as an air pollutant. In our experiments, we aimed to prepare compost tea, also known as water extract of compost, from pre-treated poultry manure in order to create a product can be utilized as liquid nutrient supply. The poultry manure source was the Baromfi-Coop Ltd. located in Nyírjákó, Hungary, where it was treated by composting. As a result of this pre-treatment of the poultry manure, its nutrient parameters improve and nitrogen is present in a form that is better utilized for plants. Furthermore, this product is suitable for further utilization and also can be the base material for a brand-new product. For this reason the effects of compost/water ratio, incubation time, low oxygen level, and extraction time on the parameters of the resulted product were studied in the frame of developing new soil-life enhancing microbial product, so-called compost tea.
Chemical parameters of the compost used as base material strongly determined the properties of the resulted compost tea, especially the ratio of the various nitrogen forms, their concentration and the salt content. It was found that adding water at a higher rate that means 1/40 and 1/50 mixing ratios results in more cost-effective production. In the experiment the compost tea were held under oxygen-poor conditions, therefore pH of the extractions decreased, which influenced the quality and quantity of their nutrient content.
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Recovery and confirmation of Haemonchus contortus from abomasal contents of roe deer (Capreolus capreolus) in Eastern-Hungary (Biharugra): A diagnostic case study
59-62Views:137Gastrointestinal parasites are ubiquitous. They occur both in wild and domesticated animals. Among such parasites of veterinary importance is the trichostrongyle worms, out of which the Haemonchus contortus species is regarded as the most pathogenic one in the small ruminant industry. The occurrence of this parasite in the sheep flock is now very well documented and an established fact in Europe, although the parasite was original of the warmer climatic region. Studies on the cross-transmission of H. contortus between the wild and domesticated animals are also on the rise although the question of the direction of transmission is still debated. This is an important area that needs to be addressed as it could potentially contribute indirectly to mitigating anthelmintic resistance. Hungary also has reported its share of the occurrence of the parasite, mainly in the sheep flock and a certain population of roe deer. The study presented here is the preliminary results of a diagnostic case study that confirms the presence of H. contortus in wild ruminant deer species that are close to the domesticated sheep population.
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The effect of the preparation method on the physical and chemical characteristics of propolis tinctures
163-168Views:246The effect of the preparation method was examined with regards to the physical and chemical characteristics of the propolis tincture, namely the extraction time and the ethanol content of the extraction solvent to the dry matter, polyphenol, flavonoid, phosphorus, calcium and copper concentration, respectively. The dry matter, the polyphenol and the flavonoid content were the lowest in the water extract of the propolis; however, significant increase was noticed depending on the extraction time. Significantly higher concentrations were found in 50 V/V% tinctures. The highest dry matter and flavonoid contents were analysed in 100 V/V% tinctures, whereas highest polyphenol content was found in 80 V/V% tinctures. However, the differences were not significant in several cases between latter tinctures. Moreover, the increase was not determined in some cases depending on the extraction time. Phosphorus and calcium concentrations were decreased depending on the increasing ethanol content, whereas copper concentration was increased up to 80 V/V%. Higher increase was found in the case of 0 and 50 V/V% extracts than in 80 and 100 V/V% tinctures. Moreover, in latter cases, no significant differences were found on several occasions, depending on the extraction time. There was no connection between the flavonoids and the calcium as well as the phosphorus content, whereas flavonoids may be made complex with copper. However, the amount of the possible complex was negligible.
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Experiences of Waste Management at Agricultural Enterprises in Hajdú-Bihar County
50-55Views:68The general tendency of the last few years has been that besides large agricultural Ltd.’s and firms, smaller agricultural enterprises also have to correspond to stricter environmental regulations.
This process suits the EU’s environmental and waste management regulations. Recently, manure, liquid manure and addle are registered as non hazardous agricultural waste in waste management in Hungary.
In the case of the amount of these wastes is larger than 200 t/year, one has to make an individual waste management plan.
Accordingly, several small agricultural enterprises have to make individual waste management plants in Hajdú-Bihar County.
This article is based on the results of these companies experiences. -
Daily soil carbon dioxide flux under different tillage conditions
141-144Views:182Over the last few years, warming of the atmospheric layer near Earth's surface is increasingly experienced and researchers have also established that concentration of numerous greenhouse gases have risen over the past two centuries value. Change is basically a legitimate process - considering atmospheric concentration as well - but the change experienced during the past centuries could not have become this critical without the contribution of human activity. Due to the nature of the greenhouse effect, the result of a very fragile, complex process is experienced currently on Earth, which can be significantly unbalanced even by a slight change. Carbon dioxide emitted from the soil is involved in the global cycle and has an impact on the greenhouse effect. The rise in soil respiration may result in the further intensification of warming. In the scope of the present study, it was examined how carbon dioxide emissions of the soil evolve over a day. The results have been established based on the comparison of the effects of different parts of the day, tillage methods and irrigation.
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Change of antioxidant compounds of spices during drying
77-81Views:150Spices and herbs have been used by humanity for thousands of years, so they are very important plants.
In this study, the change of dry matter content and antioxidant compounds of eight spices (basil, thyme, rosemary, mint, parsley, lemongrass, chives, coriander) have been examined the raw plants and in plants preserved by three different drying methods (an oven in 50–60°C; drying at room temperature; lyophilisation between -40 and -50°C, under pressure), because we wanted to see the change of the parameters.
The water content of raw plants was very high, i.e. the dry matter content was very low. By the application of the three drying methods nearly 100% of the water has left the plants, with the exception of the lyophilized basil and rosemary.
Based on the results related to the original material, lyophilized has proved to be the best treatment for the preservation of antioxidant compounds, however air drying also showed high results for some spices.
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Evaluation of Hungarian show-jumping results using different measurement variables
81-85Views:167The aim of the study was to compare different fitted models for show-jumping results of sporthorses and to estimate heritability and repeatability value. Show-jumping competition results collected between 1996 and 2011 were analyzed. The database contained 358 342 starts of 10 199 horses. Identity number, name and gender of the horse, rider, competition year, the level and location of the competition and placing were recorded in the database. To measure performance of horses, placing, number of starters and competition level were used. Competitions were categorized into five groups based on their difficulty level. The used repeatability animal model included fixed effects for age, gender, competition place, year of competition (and competition level in case of non-weighted measurement variables), and random effects for rider, animal and permanent environment effect. Variance components were estimated with VCE-6 software package. The goodness-of-fit of the models was low and moderate. Heritability and repeatability values were low for each measurement variables. The best goodness-of-fit model the weighted square root of placing resulted the highest heritability and repeatability value h2=0.074 and R=0.296.
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Awareness and adoption of a nurse sow management system among small-scale pig farmers in Nakuru County, Kenya
113-120Views:84This study aimed to assess the level of awareness and adoption of nurse sow management strategies among small-scale pig farmers in Nakuru County, Kenya. The research included a survey of pig farmers who visited the Nakuru Agricultural Show in July 2023. A total of 139 farmers were interviewed within 5 days of the show. The corresponding author interviewed the respondents in a face-to-face engagement, where questions on nurse sow management were initially drafted in English and translated into Kiswahili, i.e. the second language in Kenya. The obtained results indicated that farmers came from two main regions of the county: the northern part (54.7%, 76/139) and the southern part (45.3%, 63/139). Levels of awareness and adoption were insignificant between the two groups (χ2, p<0.05). A major obstacle to the adoption of this strategy was identified as feed challenge at 77.7%; (108/139) and market issue at 59.7%; (83/139). This study identified important aspects and limitations that should be considered when developing sustainable productivity development strategies for Kenyan pig farmers. To assist the sustainable growth of small-scale pig production, the authors suggest government measures that shield farmers against exploitation of feed and marketing components, as well as advocating for effective breeding to increase live born.
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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:274Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Examining the production parameters of European perch (Perca fluviatilis) juveniles under different lighting conditions
149-153Views:179European perch (Perca fluviatilis) is a native predatory fish in Hungary, and a promising new species of fresh water aquaculture nowadays. The European perch can be characterized by a high stress sensitivity during the intensive rearing, thus the optimization of environmental conditions has significant importance in the early life stages of fish. The aim of our study was to determine whether the light intensity and darkening of the water by humic acid affect the survival and growth performance of European perch juveniles. The experiment lasted for 28 days. Rectangular aquariums were arranged in 3 lines, the test environment consisted of 24 units. The experiment was set up with 10–10 fish per aquarium, total of 240 individuals. The average wet body weight of the fish at the start of the experiment was 1.69 grams. During the experiment, 6 treatments in 4 replicates were set up. In the first row, we set up 305.6±66.0 LUX (L), in the second row 118.0±24.4 LUX (C), and in the third row (D) 17.0±8.6 LUX illumination was set up. In each row the water of four aquariums were darkened by the addition of humic acid (H) (L, LH, C, CH, D, and DH). At the end of the experiment high survival rate was observed in all treatments. Examining the survival rate, the best results were found in the strongly illuminated treatments (L; LH- SR%=100±0.00). The observed mortality was caused by cannibalism. Regarding the individual body weight, the best results were shown by the treatments where the aquariums were illuminated with lower light intensity (C: 4.66±0.33 grams) and the aquarium water was darkened by humic acid (CH: 4.93±0.15 grams). The results of the C and CH groups were significantly better compared to the other treatments, however, they did not differ statistically from each other. The darkening of the water had a positive effect on the individual body weight of fish reared under full light (L: 3.73±0.28 grams; LH: 4.33±0.28 grams), whereas in the case of fish reared in the lowest illumination, the addition of humic acid did not affect the results (D: 3.78±0.15 grams; DH: 3.80±0.26 grams). In case of SGR (C: 3.64%/day and CH: 3.74%/day) and FCR (C: 0.84 g/g and CH: 0.78g/g) the best results were obtained by the C and CH treatments, also.
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Examinations of the carbon dioxide emission of the soil in the case of different tillage methods in a field experiment
209-212Views:229Today's global challenge is the increasing concentration of carbon dioxide (CO2) and other greenhouse gases in the air. The level of CO2 emissions may be significantly affected by the agriculture and, more specifically, the applied tillage method, even though to a lesser extent than industrial production. On a global scale, the CO2 emission of an agricultural area is insignificant in comparison to that of a large-scale plant in an area of the same size, but areas under cultivation, including arable land, have a large global area. In this paper, we investigated the relationship between applied soil tillage methods and carbon dioxide emissions in the case of different fertiliser treatments. In our experiment we examined four types of tillage with five different fertiliser effects. Comparing fertiliser treatments and tillage methods, it was found that their interaction significantly affected carbon dioxide emissions, the lowest value was obtained in the case of the 210 l ha-1 Nitrosol+N-LOCK – tillage radish treatment. Strip and tillage radish methods have relatively homogeneous, low value. -
Evaluation of water balance in apple and pear trees
193-198Views:124A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
The effect of sous-vide cooking on the antioxidant properties of oyster mushroom (Pleurotus ostreatus L.)
177-184Views:61Oyster mushrooms (Pleurotus ostreatus L.) are renowned for their antioxidant, antimicrobial, and prebiotic properties. This study explores the antioxidant characteristics, activity, and β-glucan content in freeze-dried mushroom samples, investigating the influence of sous-vide cooking. Uncooked freeze-dried P. ostreatus and three pre-cooked freeze-dried samples (70, 80, 90 °C through 4 hours) were analysed for Total Polyphenol Content (TPC), Total Flavonoid Content (TFC), Radical Scavenging (DPPH), Ferric Reducing Antioxidant Power (FRAP), and β-glucans content via HPLC and Total Dietary Fiber (TDF) via enzymatic gravimetric method. Results indicate that uncooked mushroom powder exhibited superior antioxidant capabilities compared to cooked samples. The sous-vide cooked (80 °C) mushrooms displayed the highest total phenolic and flavonoid content. Moreover, pre-cooked (70 °C) mushroom powder demonstrated the highest β-glucan content, significantly surpassing the uncooked control sample. Notably, pre-cooked groups (80, 90 °C) demonstrated significantly higher TDF levels compared to uncooked sample. This research offers valuable insights into the potential use of mushrooms as high-antioxidant, antimicrobial, and prebiotic food or feed supplements, with broad implications across various fields.
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The Role of the Digital Terrain Models in the Assessment of Surplus Water Risk at the Szolnok-Túri Plain
47-51Views:109The environmental factors to which surplus water can be assigned (topography, soil, groundwater, vegetation etc.) can be subject to special analysis and the randomness of the occurrences can be limited. The results of these procedures are surplus water risk maps of the areas, which can be utilised in land use planning. The risk map of the research site was created with overlaying digital category maps of the determining factors (hydraulic conductivity, convexity, critical probability of ground water level and land use).
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The effects of agrotechnological factors on winter wheat yield in humid cropyear
162-167Views:90The effects of crop rotation, nutrien supply and crop protection technologies, as well as the appearance of the main ear- and leafdiseases
(powdery mildew, helminthosporium leaf spot, leaf rust, fusarium) were studied on the crop yields of winter wheat variety MV
Pálma during the 2009/2010 crop year. The experiments were conducted in triculture (pea – wheat – corn) and biculture (wheat – corn), at
five nutrition levels, with the use of three crop protection technologies (extensive, conventional and intensive) at the Látókép Research Site of
the University of Debrecen, Centre of Agricultural Sciences. Our results proved that the appearance of leaf- and ear-diseases were
significant in the wheat cultures during the 2009/2010 crop year, because of the rainy, warmer than usual weather, the lodging, and the huge
vegetative mass developed. The most severe infections by the four examined diseases after pea and corn pre-crops were observed at
extensive crop protection levels, when fertilizers were used at the highest dose.
Following corn pre-crop, in the case of all the three crop protection technologies the maximum rate of wheat yield results were achieved
at N150+PK level. The highest yield was reached at intensive crop protection level (6079 kg ha-1). In triculture, in case of all the three crop
protection technologies the maximum yields were achieved at N50+PK level; in extensive technology 5041 kg·ha-1 yield, in conventional
technology 6190 kg ha-1 yield was realised, while in the intensive technological model the yield was 7228 kg ha-1.
The relationship between yield and fertilizer amounts, the rate of pathogen contaminations, crop protection technologies and pre-crops
was defined with correlation analysis in case of different crop rotations during the 2009/2010 crop year. Based on the results of the
experiment, we found that in stands after corn pre-crop strong positive correlation was established between the crop protection level and the
crop yield (0.543), the nutrient levels and the emergence of the four examined pathogens, and between the nutrient levels and the yield
(0.639). Extremly strong positive correlation was observed between crop protection and yield (0.843) in triculture. Strong positive
correlation was detected between the nutrient levels and the presence of the four examined pathogens, as well as between nutrient and
lodging (0.688). Strong negative correlation was between the crop protection level and the four examined diseases both in biculture and
triculture. -
Nest-site preference of Hooded Crow (Corvus cornix L.) in Debrecen, Hungary
13-17Views:147In the past decade, the population of the Hooded Crow has shown significant growth in Debrecen, Hungary. The aim of this study was to become acquainted with the nest-site selection behavior of Hooded Crows in urban spaces. While our research revealed that the Hooded Crow is not particular when it comes to selecting among tree species for nesting, we did notice differences regarding nesting height. Hooded Crows living in the city build their nests higher up than those living outside Debrecen’s city limits. We also discovered a slight, insignificant difference between nesting heights and tree species, which is probably due to the different characteristics of the given tree species. As for nesting heights, we found that in typical urban habitats there were no relevant differences. However, when we compared these habitats in pairs, it came to light that nesting heights -when comparing solitary trees - wood segments and tree rows - wooded segments- did show significant differences, which can be explained by the various conditions provided by the habitats mentioned.
In summary, the following results emerged from our research:
1. The Hooded Crow prefers approximately the same nesting heights in all kinds of habitats, urban environment and tree species.
2. The nesting height does not significantly depend on the habitat itself or on the tree species. -
The effect of dissolved oxygen on common carp (Cyprinus carpio) and basil (Ocimum basilicum) in the aquaponics system
89-96Views:345Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum basilicum).
Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.
In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments. -
Reference genes for livestock gene expression profiling – Literature review
81-89Views:167Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments. -
Biological control of sweet chestnut on Pécsbánya, Hungary
77-81Views:215The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
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Comparative study of special honey products and herbhoneys
117-120Views:356Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys.