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Heat treated feeds in turkey feeding
19-22Views:411The main aim of our study was to determine the effect of expanded and pelleted feeds, as well as the only pelleted feed based on wheat and corn, on the production of turkeys. In May 2006,, a fattening experiment on turkeys was started on the starter farm of Habar Ltd in Szarvas, owned by the Gallicoop Corporation. 17 day old male turkeys were involved in the fattening experiment. The experiment lasted 117 days. At that time, theanimals were 134 days old. After the experiment was completed, they were slaughtered. The following parameters were examined: growth, feed conversion ratio, carcass traits. Turkey feed were produced separately at different times. Similarly to the standard method of turkey fattening, 8 phases feeding was carried out. The fattening experiment was adjusted on male turkeys in 4×12×6 grouping (4 treatments: expanded and pelleted, and only pelleted corn and wheat feed; 12 repeats: number of pens/treatments; 6 birds/pen) 6-6 turkeys from 12 pens per each treatments were measured individually from the 17th day (starter) and at the time of each following feeding changes and mortality. The average of the group was calculated. The average daily weight gain, proportion of the given feed per pen, feeding changes and mortality were determined. The average daily feed intake and the feed conversion ratio were calculated.
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Lessons of a stripe rust epidemic in a wheat fungicide trial in Debrecen
35-46Views:306During the 2014 year a fungicide application trial was made as a part of technology development in Debrecen (East-Hungary). Both in this trial and across in Hungary a serious stripe rust epidemic developed and caused great yield losses. The first sympotoms were observed in April on one of trial plots and during some following weeks a serious infection grown up. On the base of 13 fungicide active ingredients and dosages by two spraying applications, their efficacy could be evaluated in stripe rust control. Picoxystrobin 250 g/L acive ingredient applied in 0.6, 0.8 and 1 L/ha dosages (Acanto), picoxystrobin 200 g/L + ciproconazole 80 g/L combined active ingredients in 0.5, 0.75, and 1 L/ha dosages (Acanto Plus), epoxiconazole 83 g/L, moreover protioconazole 125 g/L + tebuconazole 125 g/L combinations gave good efficacy, respectively. The yield of well protected plots were 5 to 8 t/ha, but the losses were 40-70% when fungicides with no sufficient efficacy in stripe rust control were applied. The great infection, which was observed on different grasses late in the Autumn, 2014 might forecast a further stripe rust epidemic for the 2015 year.
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Fish consumption of the great cormorant in the area of Hortobágy Fish Farm
81-84Views:350The cormorant (Phalacrocorax carbo) is a bird species that nests sporadically but in colonies, besides larger fishponds and rivers in Hungary. The number of its individuals has been increasing during the last two decades. The species eats solely fish, therefore it can cause serious depredation of the fish stocks in fishponds of intensive system and after the freezing of the ponds, in larger rivers, which are not yet frozen. The aim of our research is to reveal the damages the birds can cause on the studied areas and the extent of the losses the Hortobágy Fish Farm Co. has to realize.
Our studies were carried out between April 2012 and November 2013. During cleaning, the investigation of the crop contents of the birds and their biometric studies were conducted. The results revealed the diverse nutrition base of the species. In the samples, we have determined 300 identified fish individuals; in 289 cases, the exact fish species were determined, too. Although statistical differences were found between the given pond unit and the consuming of the corresponding fish species (P=0.05% beside), this cannot be considered as absolutely certain.
Our investigation is of considerable significance in terms of nature preservation and not least, it has substantial financial concerns too. Under the ever harder fish production conditions, the presence and thus the permanent nutrition of the bird affect the fishermen. In favour of the maintaining of the natural ecological balance and the income of the fishers, the elaboration of adequate protective and efficient preventive processes will be necessary in the near future.
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Selenium speciation analysis of selenium-enriched food sprouts
23-28Views:527In this present study, we prepared selenium-enriched pea and wheat sprouts. During our research we aimed not only to measure the total selenium content of the sprouts but to identify different selenium species.
Scientifical researches show why the analytical examination of different selenium (Se) species is necessary: consumption of all kind of Se-species is useful for a person who suffers in selenium deficit, while there is significant difference between effects of different Se-species on person, in whose body the Se-level is just satisfactory. Biological availability, capitalization, accumulation, toxicity of Se-species are different, but the main difference was manifested in the anti-cancer effect of selenium.
During our research selenium was used in form of sodium selenite and sodium selenate, the concentration of the solutions used for germination was 10 mg dm-3. Control treatment meant germination in distilled water. Total selenium content of sprout samples was measured after microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS). Different extraction solvents were applied during sample preparation in order to separate different Se-species (0.1 M and 0.2 M HCl or 10 mM citric acid buffer). We wanted the following question to be answered: Which extraction solvent resulted the best extraction efficiency? Selenium speciation analysis of sprout sample extracts was performed by high performance liquid chromatography with anion exchange column, detection of selenium species was performed by ICP-MS.
Evaluating our experimental results we have been found that significant amount of selenium of inorganic forms used during germination transformed into organic selenium compounds. There was difference between the amount of Se-species in pea and wheat sprouts and selenium uptake and repartition of selenium species were depended on Se-form used during germination. In addition the chromatogram analysis made us clear as well, that the citric acid solvent proved to be the most effective extraction solvent during sample preparation int he view of organic Se species.
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Variation of boza nutrition parameters based on raw materials
67-70Views:466Boza is a ceral based fermented drink. Raw material of boza could be rise, millet, wheat and rye. Because of the diversity of raw materilas and the method of fermentation boza might show varied quality and nutrition value.
Nowadays healthy and conscious nutrition are playing more and more important roles in our lives. People pay an ever growing attention to the nutrition value of products, such as protein- and sugar content, components with antioxidant properties, and the mineral content of the products. In our research we wanted to know how the protein,-, sugar-, total polifenol-, mikro- and macro element content of products change.
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Study of changes in the strength of connection between grey cattle cows and their offspring after weaning
129-136Views:653It is important to minimize stress for cattle because of animal welfare and profitability. This study aims to reveal how long after abrupt weaning the cow and her offspring show a need to rebuild the connection if physical barriers are removed and distances disappear. The research was carried out on Szamárhát Farm owned by the Tiszatáj Foundation, Hungary. In the autumn of 2019, thirty-one calves were weaned, randomly selected from 81 grey cattle cows’ offspring. The oldest calf was 10 months old, while the youngest one was 6 months old on the day of weaning. They were divided into three groups and reunited with the cows during the first, third, and fifth weeks after weaning. Each reunion happened in the early afternoon and the cow and her offspring spent 24 hours together again. The formation and the strength of the connection were recorded for an hour three times during this period, first in the afternoon, then in the morning, and the next afternoon. It can be seen that both parties showed the need to form the connection during the first week after weaning because the bond made between them was obvious in a short time. The connections proved to be strong. However, neither the cows nor their offsprings showed any intention to form strong connections in the third and fifth weeks. In the majority of the cases, no connection was formed.
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Effect of Storage on Fruitquality of Apricot (Prunus Armeniaca)
164-169Views:304The quality of a product is determined by numerous characteristics. As quality characteristics are polygenic, they cannot be improved easily. Moreover, there often is a negative correlation between the different parameters in the case of apricot. The firmness of fruits decreases with increasing size. The year effect also has a great influence on the expression of the potential quality of the given cultivar.
Our examinations were carried out in 2004 at Boldogkőváralja, in the cold storage plant of the Northcot Ltd. We monitored the development of fruits from fructification until harvest, and also the quality changes during storage. 100 fruits were observed from each cultivar directly after harvest. We measured height, width, weight, firmness, dry matter content and seed weight. From each cultivar, 100 fruits were stored, of which 25 fruits were observed each week. The value and the dynamics of the changes in the parameters compared to the values at harvest varied greatly for the different cultivars. Strong correlations between the parameters have not been found within one cultivar either. The heavier fruits did not lose more weight, either in absolute value or relatively, than the lighter fruits. The increase in the firmness of fruits was not accompanied with a desirable decrease in the dry matter content. -
Magnesium uptake dynamism of maize (Zea mays L.) on prairie soil
83-89Views:188Different influence factors on the magnesium (Mg) uptake in case of three maize hybrids with different long vegetation period have been investigated at the Experimental Station of the University of Debrecen, Centre of Agricultural Sciences and Engineering, at Debrecen-Látókép. The soil of the experiment is a calcareous chernozem, based on loess, with high fertility, that is characteristic for soils of the region Hajdúság.
Upon irrigation is the experiment divided to main plots, by different hybrids into sub-plots, while treatments of five nutrientsupply levels with fixed N:P2O5:K2O rate (beside control)mean sub-subplots. Soil samples were taken from the upper, cultivated soil layer 3 times during the year 2008. Their pH has been measures in a 0.01 M CaCl2-solution and their Mg-content from the same solution and from ammonium-lactate acetic acid (AL) extract. Plant samples were taken seven times in the vegetation period, of which we measured the Mg-content. Beside this, the during the
vegetation period by maize biomass extracted Mg-amount has been calculated using fresh and dry matter weights. The effects of irrigation, hybrids and nutrient-supply levels on the soil pH and on the AL- and CaCl2-extractable Mg-amount have been studied, as well. After that I tried to find a correlation between soil pH and the Mg-content of soil determined in different extractants, beside this between the by the two solutions extracted Mg-amount. -
Using the principles of precision animal husbandry in fishbreeding
283-287Views:562Aquaculture species such as fish, crayfish, molluscs and plats are a wide range of products, with continuously growing demand worldwide. The reasons for this is that they are cheap and easy-toraise protein sources, thus having significance in food supply especially in developing countries in tropic regions, moreover, the premium category foodstuffs in developed high income countries are also belonging to this category. World annual total production of 164 million tons (2009) are made up of two sources: 1) marine and inland fisheries landings that are stagnating for several years and 2) aquaculture which is growing dynamically with annual 6% rate between 2000–2009. The latter is accounting for nearly 45% of the total supply due to the depleting marine stocks caused by overfishing. Aquaculture is growing continuously also because the production is safe and can
be planned well.
Intensive fish production systems are the representatives of precision animal production, several types exist and widespread worldwide. The modern computerized temperate water recirculation plants with several thousand m3 capacity are widespread also in Europe because they make it possible to produce even the most valuable species whole year round. A key issue in the technical/feeding outlay is to meet the demands of the cultured species the best and the operation of the system is to be safe and cost-effective. One condition for this is intensification: enable to produce more product per unit resource input or effort. The facilities need significant amount of energy, thus renewable energy sources are to be favoured for which Hungary has comparative advantages. -
Examination of the population density and sowing date of different maize genotypes in the Hajdúság region
111-115Views:451The experiment was carried out 6 km from Debrecen, next to the main road 47 on a homogeneous field on brown forest soil. Five corn hybrids were tested in the trial (DKC 4795, DKC 4995, KWS Kornelius, NK Cobalt, PR37 N01) at three different sowing times (early – 5th April, average – 21st April, late – 10th May). At each sowing time, three different plant densities were applied (modest – 58 500 plants ha-1, average –70 200 plants ha-1, high – 82 300 plants ha-1). The agrotechnics applied
in the experiment satisfied the requirements of modern corn cultivation.In the study, the best yield result was achieved with the early sowing time out of the three examined sowing times (11 315 kg ha-1), which was significantly different (LSD5%=495 kg) from that of the average sowing time (10 690 kg ha-1), however, there was no statistically justifiable difference between the yield results of the early and the late sowing times. There was a significant difference also between the average and late sowing time. Our results indicate that the different sowing times resulted in a different flowering times. Consequently, the stands of early and late sowing time reached this critical stadium of growth under proper climatic circumstances (precipitation: 39 mm and 136 mm, average temperature at flowering: 18.1 oC and 20.3 oC), while flowering in the case of the average sowing time of 21st April was in the first half of July and the average temperature at flowering was warmer (23.2 oC) with only 10 mm precipitation.
In the experiment, the plant density response was also examined. According to the measured data, four of the five hybrids responded badly to the increasing plant density. We found that the plant density of 58 500 plant ha-1 gave the largest yield results (DKC 4995 11 794 kg ha-1 – NK Cobalt 10 998 kg ha-1, average of five hybrids: 11 430 kg ha-1), while the lowest yields were obtained at the plant density of 82 300 plant ha-1 (KWS Kornelius 11 037 kg ha-1 – NK Cobalt 10 019 kg ha-1, average of five hybrids 10 720 kg ha-1). The difference between the two plant densities was significant (LSD5%=494 kg), however, the 70 200 plant ha-1 plant density did not show any statistical difference from neither the 58 500 ha-1 nor from the 82 300 plant ha-1 stands. When examining the data of the hybrids separately, we found that there was a significant difference between the average yield of the lowest and highest plant densities only in the case of three (DKC 4795, DKC 4995, NK
Cobalt) out of the five hybrids (DKC 4795: 11 757 kg ha-1 – 10 857 ha-1 where LSD5% =816 kg; DKC 4995: 11 794 kg ha-1 – 10 738 kg ha-1 where LSD5%=853kg; NK Cobalt: 10 998 kg ha-1 – 10 019 kg ha-1 where LSD5%=630 kg ha-1), while a significant difference between the second and third plant densities was observed only in one case (DKC 4995: 11 726 kg ha-1 – 10 738 ha-1 where LSD5%=853 kg). In all other cases, there was no statistical difference between the different
plant densities. -
Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:280Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Study of factors controlling the amount of 0.01 M CaCl2 extractable Norg fraction
437-449Views:332The use of new methods describing the “readily available” nutrient content of the soil is spreading on a global scale. The 0.01 M CaCl2 extractant is a dilute salt solution in which the easily soluble inorganic (nitrate-N and ammonium-N) and organic N fractions, P, K and micronutrients are also measurable. The 0.01 M CaCl2 has been tested in the University of Debrecen, Institute of Agricultural Chemistry and Soil Sciences since the 90’s. The results of the researches related to organic N fraction, performed in the last decades, and the results of the present study (originating from the long-term experiment of Karcag, 2007–2009) can be concluded as follows:The measurement of easily soluble and oxidizable organic nitrogen (Norg), besides inorganic fractions, could improve the nutrient management.The amount of the Norg fraction is determined by the soil conditions, therefore it is considered to be a site-specific parameter.Management practices and cropyear affect the amount of Norg as well. The present research confirmed that, the effect of fertilization on the amount of Norg can be explained by the changing of the yield (related to total biomass production), while the effect of cropyear is related to the differences in mineralization circumstances and yield as well.The measurement of the Norg fraction is increases the accuracy of N-supply, therefore it could prevent the environmentally harmful excess N application as well. -
Early evaluation of use of fermented chicken manure products in practice of apple nutrient management
195-198Views:646According to the Green Deal efforts, the importance and relevance of organic fertilization will increase in the near future. Therefore, the investigation of the effects of different organic fertilizers on soil productivity and nutrient supply is a priority area of agricultural research. Organic fertilizer experiment was conducted in an eight-year-old apple (Malus domestica Borkh.) orchard at Debrecen-Pallag. In the trial Pinova cultivar was used. In this study, two different fermented chicken manure products were added to the soil (in 20 cm depth) to test their effects on soil nutrient status, plant uptake and fruit quality. It was found that the applied treatments slightly increased the pH and nutrient levels in almost all cases, but significant effect was not observed in all treatments compared to the control. Leaf nutrient contents (N, P, K, Ca and Mg) were measured in the experiment. Leaf nutrient status was not affected by the fermented chicken manure treatments. However, used treatments had strong effects on the fruit characteristics and inner parameters, such as fruit diameter and Brix value. Moreover, it was established that the applied organic fertilizers increased the yield significantly.
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Large Scale Measurement of the Total Antioxidant Capacity of Different hungaricum Fruit and Vegetable Samples with Photochem Chemiluminometric Device
300-304Views:255In our present study, we performed a quick test of the antioxidant capacity of domestic fruit and vegetable cultivars with a PHOTOCHEM® instrument (Analytik Jena AG, Germany). The instrument is special because it can measure both the total lipid-soluble antioxidant capacity (ACL method) and the total water-soluble antioxidant capacity. Another advantage of the system is its quick measurement. With similar instruments, such measurement can take several hours, while PHOTOCHEM® can perform it within a few minutes.
In our model studies, we examined the changes in the antioxidant capacity of sour cherry, green pepper, carrot, pumpkin, red beet and Brassica sp. cultivars. We aimed to determine how suitable the instrument is for quick, routine measurements in the case of the different horticultural products and which species have high antioxidant capacity values.
On the basis of our examinations, we can state that the PHOTOCHEM® system is suitable for routine, semi-automated measurements of the total antioxidant capacity at several vegetables and fruits. Besides this, the system can be used for testing the quality of food products, for monitoring storability in post-harvest technologies, and for special breeding programmes and for physiological and biotechnological studies. -
The Effect of Forecrop and Plant Protection on the Pathology Parameters and Yields of Winter Wheat
84-89Views:501We carried out our experiment in the cropyears of 2000/2001, 2001/2002 and 2002/2003, on calcareous chernozem soil, at the experimental site of the Debrecen University Farm and Regional Research Institute, at Látókép. We examined the disease resistance and the yield quantity of Mv Magvas variety by adopting different forecrops and plant protection technologies, at 30+30 N level and at normal cereal row spacing. We applied two forecrops (wheat and pea) and two plant protection technologies (extensive and intensive). We measured the rate of infection by population survey in the first ten days of June.
In the course of our examinations, we found, that the rate of powdery mildew infection was higher in the thicker population sown after pea forecrop in all three years, as powdery mildew is not a typical cereal disease.
The infection rate of leaf mildew and DTR (Dreschlera tritici-repentis) was higher after wheat forecrop in all examined years, because these are typical wheat diseases and infection centres in the soil promote the spreading of these diseases. However, it was possible to parry the adverse effect of forecrops by intensive plant protection.
Due to the chernozem soil, wich has good water management features, and due to the good preparation of the seedbed, the effect of forecrops on yield quantity did not appear in the examined years. The quantity of the yield was only slightly larger after pea forecrop in the cropyears of 2000/2001 and 2002/2003 than after wheat. Nonetheless, the data of technical literatures state that the yield quantity can be larger, even by 15-20%, after pea forecrop.
In the course of intensive plant protection technology, we applied systemic pesticides, while in the course of environmentally sound technology, we used contact pesticides of sulphur content. In those populations that were treated with environmentally sound plant protection technology, infection rate was higher in all three years.
Yield quantities were somewhat lower in the course of applying extensive, environmentally sound technology, because diseases appeared in these populations to the higher degree. Powdery mildew does not, but leaf mildew and Dreschlera tritici-repentis have a significant yield decreasing effect. With appropriate, well-selected fungicides, we were able to keep every leaf diseases well in hand, and the rate of infection was almost independent of the influence of the breeding year. -
Additional data for the evaluation of coat colour varieties in the Hungarian Grey cattle
44-47Views:400When preserving genetic resources, one of the most important tasks is to conserve as much of the given gene pool for the future generations as we can. Therefore, traits that have no economic value at the moment should also be conserved. The great variety of coat colours seen in the Hungarian Grey cattle form part of the world’s genetic heritage. In order to maintain the world’s genetic diversity, we have to maintain these varieties, as well. The different coat colour varieties were determined – in both sexes and in several age groups – with a Minolta Chromameter CR-410 in an objective way. We found that the rate of the three main coat colour varieties of calves: the light reddish, the reddish and the dark reddish were 26%, 52% and 22%, respectively. Statistically significant differences were found between the L*a*b* values of the reddish coat colour of the Hungarian Grey and the red coat colour of the Limousin calves. The rate of the crane, the grey and the light grey coat colour varieties were determined in the measured female groups. The L*a*b* values of the Hungarian Grey and Maremman bulls’ coat were compared and evaluated.
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Estimating Genetic Parameters using a Random Regression Model
53-55Views:484One of the most important part of the genetic evaluation using a random regression model is the estimation of variance components. This is the topic of many papers because the large computational costs. We can use restricted maximum likelihood (REML), Gibbs sampling and ℜ method for the estimation of genetic parameters. The variance components are necessary to calculate the heritabilities and repeatabilities.
The aim of our paper is to estimate the variance components using a random regression repeatability model from test day data set of Hungarian Holstein-Friesian dairy cows and to analyse the change of additive genetic and permanent environmental variance, heritability and repeatability over lactation. -
Effect of sowing technology on the yield and harvest grain moisture content of maize (Zea mays L.) hybrids with different genotypes
17-22Views:277From the aspect of the efficiency of maize production harvest grain moisture content shall be considered beside the amount of harvested grain yield. Hybrids with different genotypes and vegetation period length lose their moisture content different that is affected by row spacing and plant density – among agrotechnical production factors – depending on the given crop year. In the present research work three crop years with different weather conditions were studied (2013, 2014, and 2015). The small-plot field experiment was set up at the Látókép Field Research Centre of the University of Debrecen, Centre for Agricultural Sciences with four replications on a chernozem soil type. The effect of three factors was analysed in the experiment on yield amount and its moisture content. Factors were row spacing (45 and 76 cm), plant density (50, 70 and 90 thousand plants ha-1), while hybrids were of very early (Sarolta: FAO 290), early (DKC 4014: FAO 320, P 9175: FAO 330, P 9494: FAO 390) and medium (SY Afinity: FAO 470) ripening.
In the crop year of 2013 the highest yield was produced – regarding the average of the hybrids – by the application of a row spacing of 45 cm (4.5%, 673 kg ha-1), however there was no significant difference between the yield of the populations of different row spacings. Significant difference (14.9%, 1751 kg ha-1; 6.3%, 583 kg ha-1) could be found in case of yield between different row spacing applications in 2014 and 2015. The effect of insufficiently distributed low amount of precipitation and lasting heat days in 2015 could be revealed in yield amounts and harvest grain yield moisture content results that were lower than in the previous years. In 2015 grain yield moisture content varied between 10.3 and 13.9% in case of a row spacing of 45 cm, while by 76 cm between 11.0 and 13.9%.
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Study of the Interaction Between Common Carp (Cyprinus carpio) and Silver Crucian Carp (Carassius auratus gibelio Bloch)
9-12Views:479Some of the main questions in the fish farming are the good manuring and the success of the feeding. For the best result is very important that only those fishes take place in the fishponds what we want. It seems to be easy but difficult to do. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the rearing season, weekly test fishings were made to control the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower growth rates and productivity in common carp. In the control pond (without silver crucian carp), the average weight of the common carp was 25 g and the yield/hectar was over 900 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 9 g and the yield/hectar was only 158 kg.
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Heat treatment and microbial digestion of poulty feather for biogas production
215-219Views:424The aim of this research was the elaboration of the technological parameters of heat pre-treatment and microbial digestion of poultry feathers for biogas production. Feathers were treated at 70, 100, 140 °C, and subsequently digested by Bacillus licheniformis, or keratin disintegrator bacteria. Investigations focused on the optimalization of parameters influencing poultry feather biodegradation. The optimal range of pH, temperature,
feather size and bacillus:feather ratio were determined in the experiments, as well as the analysis of relationship between the examined parameters. In order to be able to track the dynamics of the biodegradation, we determined the extinction level of the liquid phase of the biodegraded material in the different experimental treatments. The results showed that the rate of hydrolysis was significantly higher in the treatments with bacteria than in the treatments without it. Regarding the pretreatments at 70, 100 and 140 °C, the digestion of feather was the most intensive at 70 °C. The most extensive digestion was observed in case of 1:3 feather:water ratio. The highest intensity of feather digestion was
detected in the treatment with 1% microbe ratio. -
The impact of different fertilization methods on some microbiological soil characteristics
119-126Views:314In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated.
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly.
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer. -
Plant growth analysis of wheat populations in a long-term field-experiment
147-151Views:417The experiments were conducted as part of the long-term trial adjusted, in triculture (pea-wheat-corn) and biculture (wheat-corn), at three nutrition levels, with the use of one crop protection technologie (conventional) at the Látókép Research Site of the Centre of Agricultural Scienses, University of Debrecen, on a chernozem soil. The wheat variety used in the long-term trial was GK Csillag, which was sown at 5,8 million germs/ha.
The effect of pre-crops and nutrient-supply levels on some growth-parameters (LAI, HI, LAD), just as SPAD-values and yield amounts of winter wheat has been investigated in this experiment. We tried to find out the extent of relationship between the different parameters, so we determined the relationships between different nutrient-supply levels, yield amounts, LAI- SPAD- and LAD-values – measured in the crop-year of 2010–2011 in different crop rotation systems – by using correlation analysis. It has been stated both in case of bi- and tri-culture crop-rotation systems that different fertilizer dosages had significantly affected the leaf area index dynamics and its maximal value, and that increasing N+PK fertilization has
significantly increased the duration of leaves, as well. The highest SPAD-values were measured during the flowering and grain filling stages. However, we haven’t revealed significant differences between all fertilizer treatments. In case of the bi-culture crop-rotation system harvest index values showed an increasing tendency parallel to the increasing nutrient-supply levels, while in case of the tri-culture system this tendency was rather decreasing. However, these differences were
not significant. Parallel to the increasing fertilizer dosages yield results were increased in a significant extent. At the same nutrient supply-levels 2088–4615 kg ha-1 higher yields were measured in the tri-culture than in the bi-culture system. The correlation analyses have confirmed that all of the investigated parameters (yield amount, LAI, SPAD, LAD) had almost in all cases close positive correlation to the nutrient-supply level and the yield amount in both crop-rotation systems. These results have confirmed that the leaf area, the leaf duration, the SPAD-values and the fertilization have altogether resulted in the production of maximum grain yields. -
The effect of NPK treatments on the Cu and Fe content of winter wheat (Triticum aestivum L.)
31-34Views:533In this study the effect of N, P and K nutrients on the Cu and Fe content of winter wheat (Triticum aestivum L.) grains was investigated in a long-term fertilization experiment set up in Nagyhörcsök. Samples were also harvested from four experimental stations of the Hungarian national long-term fertilization trials. These are the following: Bicsérd, Iregszemcse, Karcag, and Putnok. Plant samples were collected in 2005 which was very wet. Our results from Nagyhörcsök were compared with the Cu and Fe content of samples which were harvested from control plots of other experimental stations. The Cu and Fe content of grain samples were measured using inductively coupled plasma mass spectrometer (ICP-MS) followed by digestion with HNO3-H2O2 solution. All data were subjected to ANOVA, and when significant differences (P<0.05) were detected, Duncan’s test was performed to allow separation of means.
The main conclusions are as follows: Cu and Fe content of wheat grains was higher and higher in every NPK treatments. Samples were harvested from the control plots of Iregszemcse and Bicsérd have higher Cu content than the treated samples from Nagyhörcsök.
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New wonder of an old hormone: Hungarian sour cherry varieties as natural melatonin sources
65-72Views:408Agriculture has always been an important role in economy, food supplies, sustainability of society During our experimental work an effective extraction procedure has been developed for melatonin. Further chromatography was developed the quantitative and qualitative determination of melatonin. A compound that was equal to the standard was purified from the extract by preparative HPLC technique and the structure of the purified sample was confirmed by MALDI-TOF-MS and NMR analysis. Based on our measurements, harvested in the state of biological maturity Hungarian sour cherry cultivars contain high levels of melatonin.
Our results show that "VN4" variety has extremely high melatonin content, 9.893 ug g-1 and suggest that "VN4" which were selected from the "Bosnyák" varieties is melatonin accumulating. The average value of the melatonin content of Hungarian sour cherry cultivars is 2.319 ug g-1.
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Examination of microsatellite markers of Dorper sheep breed
57-61Views:643Number of not woolly and molty sheep exceeds 60 million throughout the world. Their numbers and their importance is growing, still they have appeared in the past two decades all over in North-America, Australia, New-Zealand and also in Europe. The South African Dorper has been a pioneer among them in Hungary. It was introduced in 2006 in the country. The Dorper sheep is the second largest breed in South Africa, which was developed from the crossing of Dorset Horn and the Blackhead Persian. The aim of the EU Member States in terms of this specific breed is increasing the small populations, improving the productive qualities, in addition to this avoiding inbreeding. However, finding appropriate breeding stock is difficult due to the small size of available populations and also to the suspected common of origin. With the help of various molecular genetic methods we could get a total view of the genetic background of these flocks. Nowadays the most commonly known and used genetic markers are microsatellites, because their applications give fast, accurate and easily reproducible results. There is no specific descriptive information on the genetic background of Dorper populations in the various EU countries , also regarding diversity between populations. Therefore in our work we want to optimize the conditions of applicability of 31 selected microsatellite reactions as a first step of mapping the entire genetic background of the different EU Dorper populations.