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Spectral analysis of stress symptoms caused by apple powdery mildew (Podosphaera leucotricha
83-88Views:475An orchard can be examined on the basis of spectral data, using methods with which the reflected radiation can be divided into a large number of (several hundreds) small spectral channel (some nm). Calculated on the basis of such hyperspectral data from different index numbers the water supply of foliage conditions can be well characterized.
The research site is an intensive apple orchard, which located in University of Debrecen, Agricultural Sciences Centre, Farm and Regional Research Institute at Pallag. During my experiments the preliminary evaluation of spectral, non-invasive measurement method are carried out for detecting stress symptoms caused by Podosphaera leucotricha.
Based on the results narrow band greenness indices (NDVI705, mNDVI705, mSR705 and REP) can be used for determination of diseased canopy and for the detection of stress symptoms of Podosphaera leucotricha,. These statements can be utilized in precision plant protection systems, since it can be a basis for such integrated active sensors with LED or laser light source, measuring reflectance at the certain spectral range, which can facilitate real time status assessment of orchards and can control precision fungicide utilization.
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Modelling forestation alternatives
35-41Views:554Agroforestry systems are part of the history of the European Union rural landscapes, but the regional increase of size of agricultural parcels had a significant effect on European land use in the 20th century, thereby it has radically reduced the coverage of natural forest. However, this cause conflicts between interest of agricultural and forestry sectors. The agroforestry land uses could be a solution of this conflict management. One real – ecological – problem with the remnant forests and new forest plantation is the partly missing of network function without connecting ecological green corridors, the other problem is verifiability for the agroforestry payment system, monitoring the arable lands and plantations.
Remote sensing methods are currently used to supervise European Union payments. Nowadays, next to use satellite imagery the airborne hyperspectral and LiDAR (Light Detection And Ranging) remote sensing technologies are becoming more widespread use for nature, environmental, forest, agriculture protection, conservation and monitoring and it is an effective tool for monitoring biomass production.
In this Hungarian case study we made a Spatial Decision Support System (SDSS) to create agroforestry site selection model. The aim of model building was to ensure the continuity of ecological green corridors, maintain the appropriate land use of regional endowments. The investigation tool was the more widely used hyperspectral and airborne LiDAR remote sensing technologies which can provide appropriate data acquisition and data processing tools to build a decision support system.
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Application of advanced environmental assessment methods in orchard management
221-225Views:341Our reseaches were carried out in apple and pear orchards at Farm and Regional Research Institute in Pallag of the University of Debrecen and Pear Gene Reservoir in Újfehértó. Aim of this study is to interpret and analyse field studies with the aim of a GIS based database. Furthermore, beside field measurements, airborne and field hyperspectraldatacollection and analysis were also made to facilitate special watermanagement and irrigation related surveys. The integration of unified, geoinformatics systems with high spatial resolution and calibrated airborne hyperspectral data are appropriate tool for decision support systems, which support the continuous update and actualization of the changing cropping data, the analysis of cropping results in a unified complex data system, the acquiring of agro environmental subsidies, the establishment of monitoring system, and the optimization of irrigated fruit production.
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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:586Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Evaluation of population parameters and biometric data of an Eurasian Collared Dove (Streptopelia decaocto Friv.) population in the Great Plain of Hungary
89-92Views:578The Eurasian Collared Dove shows different patterns in density all over Hungary. In some cases, the population has decreased to a lower level, but there are habitats where the population remained stable. Several factors were taken into consideration during the evaluation of this phenomenon. These publications state that the increased urbanization of corvids, the lack of food sources, and the changes in nesting sites caused the decrease in numbers. During our research, we intend to contrast the differences in population structure of Collared Doves that differ from each other in the density of predators presented at nesting sites. As a first step, in this paper, numerous samples (n=118) were taken from a population that is less influenced by corvid species’ nest predation. Our prediction was that the population parameters remained at the level of 1980’s, in which the population of Eurasian Collared Dove has reached its maximum, according to further publications.
During the evaluation process, our data were collected from hunting bags near Nyíregyháza. Results were compared with former publications. We stated that the population structure, such as age and gender distribution remained stable in the evaluated population. In addition, morphometric factors of the collected individuals were also measured, such as weight, wing length, 3rd primary feather, and tail length, which were compared with former surveys. During statistical tests no significant difference was revealed among population parameters, but there were significant differences between most of the morphometric data, (p>0.05).
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Responses of pea (Pisum sativum L.) to the rising atmospheric concentration of carbon-dioxide
185-188Views:455The atmospheric concentration of carbon dioxide increases from decade to decade in increasing pace. In 1957, atmospheric carbon dioxide levels were around 315 ppm, while in 2012 it amounted to 394.49 ppm concentration. In parallel, the global temperature is rising,which is projected to average 1.5–4.5 °C. The carbon dioxide concentration is a key factor – in interaction with the light – affects the plant's photosynthesis. Among the various factors significant interactions prevail: environmental factors affect - the growth and the development of plants, leaf area size and composition, the function of the photosynthetic apparatus, the duration of growing season.
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Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)
127-131Views:719Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and yield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.
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Utilization of the field experiment results of University of Debrecen in the development of maize-based bio-ethanol production
55-57Views:489Maize is currently the single raw material of bio-ethanol production in Hungary. The aim of our examinations is the observation of yield and
nutritional characteristics of commercial maize hybrids in Hungary from the aspect of efficient bio-ethanol production. We set up a
randomized block field trial. We determined the starch content and starch yield (t ha-1) of the 51 maize hybrids involved in the field trial.
In laboratory conditions, we examined the amylose and amylopectin ratio and the amount of resistant starch of the selected 20 maize hybrids.
According to our results, there is a significant difference between the starch yield the amylose component of the starch content and resistant starch of the examined maize hybrids. Our studies reveal that maize as a raw material must be selected based on the cultivation objectives. If the objective is bio-ethanol production, detailed knowledge of starch content is necessary. There is a significant difference among commercial maize hybrids in Hungary in terms of characteristics determining the producible amount of bio-ethanol. -
Association analysis of TNNI1/XbaI polimorphism on carcass quality in hybrid pigs
59-62Views:567The contractile protein, which is encoded by troponin I 1 (TNNI1) gene, is located on the thin filaments of slow fibres in striated muscle. TNNI1 protein is a part of the troponin complex which plays an important role in regulation of muscle contraction by preventing actin-myosin interaction in absence of calcium. According to biological role, this gene can be potential marker for meat production related traits. The aim of this study is to define whether the previously reported gene polymorphism (EU743939:g.5174T>C) is connected with the slaughter traits measured in a standard slaughterhouse of the examined four-line European hybrid. The study included data from 404 gilts and barrows from 2 different samples. The polymorphism was detected using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method with XbaI restriction enzyme. In this study the allele frequencies were found as follows: C: 0.84 and 0.808; T: 0.16 and 0.192. Based on result of the present study no significant impact of polymorphisms on production parameters was found.
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Leaf and root evaluation of bioactive compounds of different beetroot varieties
135-139Views:406The importance of beetroot lies in the numerous health benefits, attributed to its wide-ranging array of minerals, vitamins and bioactive compounds which play a key role in the human diet. Generally, beetroot is consumed for its root, however, its leaves also contain a good amount of bioactive compounds. The experiment was carried out with four beetroot varieties in order to compare the amount of bioactive compounds in the root and the leaf. The analyzed data were total dry matter content, total polyphenol, flavonoid and vitamin C.Results have shown that roots contain the highest amount of bioactive compounds. Nevertheless, leaves can be also considered a significant source. Therefore, consumption of the young leaves is highly recommended due to their availability after thinning or at maturity stage (60 days after the germination). Of the examined varieties, ‘Libero’ demonstrated the highest total polyphenol, flavonoid and vitamin C values, although it was found to be statistically insignificant. -
Changes in the relationship between Hungarian Grey cows (Bos primigenius taurus hungaricus) and their calves in the period from calving to four months of age
85-90Views:546The ability of cows to care for their offsprings is a very important trait concerning beef cattle. Maternal instincts are highly influenced by breed, specific cow, and social and rearing environment. In 2020, at the Szamárháti farm of the Tiszatáj Public Foundation, we selected 15 dam-calf pairs and studied them for four months from the birth of the calves. We grouped calf-rearing behaviors according to strength and analyzed the related changes during the program. At the beginning of the relationship, "standing close to each other" (53%) and "suckling" (24%) characterized the pairs. After the first month, "standing or lying separated" grew by 69%, and "vocalization" by both animals fell by 66%. Even though the daily frequency of suckling decreased by 34% as the calves grew older, it remained a typical element of the relationship. Over time, the bond between the animals gradually weakened. This natural process of separation takes a month to complete.
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Evaluation of water balance in apple and pear trees
193-198Views:610A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
Effect of allithiamine on the level of hyperglycaemia-induced advanced glycation end products
41-44Views:539Diabetes mellitus is a rapidly growing public health burden in both developed and developing countries. Diabetes mellitus is accompanied by hyperglycaemia, which can cause tissue injury by several mechanisms. One of these is the formation of advanced glycation end-products (AGEs). In this study, the effect of allithaimine, a fat-soluble thiamine derivative, was investigated on hyperglycaemia-induced AGEs levels using human umbilical cord vein endothelial cells (HUVECs) as a hyperglycaemic model. HUVECs were isolated by enzymatic digestion, characterized by flow cytometer and treated 30 mM glucose plus allithaimine or thiamine or cell maintenance medium as control. Allithiamine was synthesized and purified. The structure of the synthesized and isolated compound was verified by reverse phase HPLC and MALDI-TOF. AGEs were evaluated by ELISA. Collectively, our results indicate that allithiamine can reduce level of the hyperglycaemia-induced AGEs similar to thiamine.
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Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:260Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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The influence of fertilization on the soil characteristics of a calcareous chernozem in a long term experiment
47-52Views:288In the long term fertilization experiment of the University of Debrecen, Centre for Agricultural and Applied Economic Science(CAAEC) (Debrecen Látókép), the effects of a 25-year-long fertilization were examined in terms of some chemical and microbiological properties of soil. With the growing doses of fertilizers, the available nutrient content of soil increased. At the same time the pH significantly decreased, while the hidden acidity increased. Moreover, the ratio between the soil bacteria and microscopic fungi, and the occurrence of microbes also changed. The number of sensitive physiological bacteria groups decreased dramatically. These changes indicate the reactions of living organisms; they correspond to the „resistance stage” of stress effects, but in the case of nitrifying bacteria, they reach the „exhaustion stage”.
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Comparative study of a winter wheat variety and hybrid sown after different pre-crops on chernozem soil
63-69Views:499Wheat production is a determining branch within Hungarian crop production (produced on nearly one million hectares). Weather anomalies caused by climatic change confirmed the importance of the biological background (variety, hybrid) in wheat production. The adapting ability and reaction of different wheat genotypes towards nutrient supply were studied in a long-term field experiment on chernozem soil type in the case of different pre-crops (sunflower and maize). According to the experimental results of the vegetation of 2017/2018, the yield of the variety Ingenio sown after the sunflower as previous crop ranged between 4168 and 8734 kg ha-1, while in the case of maize as previous crop, this value ranged between 2084 and 7782kg ha-1, depending on the applied nutrient supply level. The studied genotypes produced rather significant yield surplus as a response to the application of mineral fertilization (4.6–5.1 t ha-1 after sunflower and 5.7–6.3 t ha-1 after maize). Optimal mineral fertilizer dosage was determined by both the genotype and the pre-crop. N-optimum values of wheat genotypes was determined using regression analysis. In the case of the variety Ingenio sown after sunflower, the optimum range was N144-150+PK, while after maize, it was
N123-150+PK, respectively. For the hybrid Hyland, these optimum ranges were N114-120+PK, just as N150-153+PK, resp. The application of optimal mineral fertilizer dosages improved water utilization of the studied wheat genotypes to a significant extent. WUE values of the control, unfertilized treatments ranged between 4.1–8.3 kg mm-1, while in optimal fertilizer treatment, it ranged between 15.5 and 17.4 kg mm-1. -
Daily behaviour of Hungarian Grey Cattle under range grazing conditions
45-48Views:368Cattle behaviour on rangeland depends on external factors, such as grass allowance and quality, temperature, net solar radiation, distance from water-source, wind speed and direction, air pressure changes and the applied breeding technology. Our research is based on previous empirical observations and modern methods to analyze the behaviour of the Hungarian Grey Cattle. Today it is crucial to use cost-effective solutions in modern beef cattle farming therefore we introduce a cost-efficient method to study and follow cattle herds. We are studying relation between traveled daily distance and air pressure. The various weather fronts influence behavioural characteristics and traveled daily distance. According to our hypothesis, the pressure-change and the wind direction has significant effect on cattle activity on pasture. As the different air masses alter the barometric conditions and unbalance the neuroendocrine system, indirectly cause relaxed or agitated behaviour
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Evaluation of the correlations between the normalized difference vegetation index (NDVI) and yield in a seasoning paprika (Capsicum annuum L. var. longum) stand
45-49Views:714The aim of our examination was to evaluate the correlations between the normalized difference vegetation index (NDVI) and yield, as well as to examine the possibility of yield estimation basedon NDVI in a seasoning paprika population.
Significant correlations were observed during the examination of the correlation between NDVI and yield. Furthermore, it was concluded that yield can be estimated with a 6.6–8.3% mean error based on the regression equations. No significant difference was shown between the error of estimations performed with various regression types and that of the estimations performed at various dates. For this reason, the identification of the optimum estimation method and the determination of the optimum date for estimation call for further examinations.
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The role of green manure crops in Hungarian plant production
49-53Views:571According to the data of KSH (Hungarian Central Statistical Office), the sowing area of cereals in the crop year 2016 was over 2.56 million ha, on which winter wheat and maize were produced in a rate of around 50–50%. Regarding these data it is obvious that the domestic cropping structure has been simplified and become unilateral. This unfavourable crop rotation system causes several problems. The number of Hungarian livestock decreased in the past decades. The amount of manure was 24 million tons in 1960, but only hardly 4.5 million tons of organic manure was applied in 2016. Therefore, the importance of other possibilities, alternatives for organic matter recovery have become enhanced. This is especially important from the aspect of sustainable plant production. The fact that the European Union has introduced new directives for subsidiaries in 2015 has to be noted as well.
The objective of the set experiment is to find new technological solutions that are suitable for the execution of sustainable plant production by inadequate crop rotation, organic substance recovery conditions or under more unfavourable climatic conditions.
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The current status, challenges and prospects of native pig production and large-scale farming in Laos
155-161Views:798This review aimed to investigate the potential native pig development and the trend that large-scale farming may influence native pig production in Laos. The review found that native pigs were estimated to be around 88% of the total pig population in Laos in 2023. Commercial pig farming was found only in the main cities, particularly the central and southern provinces, which increased from 578 in 2019 to 703 farms in 2022 and 737 farms in 2023. Due to the serious economic crisis starting in 2021, many Lao pig producers stopped their businesses, leading to the decline of the commercial pig population by almost 30% in 2023 compared to 2022. The average pork per capita consumption in Laos in 2021 was estimated at about 14.2 kg. Though not as high as the backyard, commercial farms still contribute essentially to the pork supply in Laos (12%), increasing the volume from 87,628 tonnes in 2015 to 137,775 tonnes in 2024. The main native pig producers are small-scale rural farmers who have not yet improved productivity or reproductive performance, and the main purpose of their production is household consumption rather than market orientation. However, farmers, mainly in the main cities, improved them by cross-breeding native pig sows with Duroc boar for better lean meat. The main challenges for native production in Laos include poor farm setup and management, high cost of commercial feed, and poor breeding and genetic management. Therefore, creating awareness of the benefits of setting up a pig farm and management, providing better option strategies for utilising locally available feeds, and providing better strategies on pig breeding could improve native pig production in Laos.
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Effects of long-term K fertilization and liming on the extractable and exchangeable K contents of a Haplic Phaeosem soil
141-145Views:235Effects of regular K fertilization and liming on the easily extractable K content of a Haplic phaeosem soil determined in 0.01 M CaCl2
and AL (traditional method in Hungary) were examined in the B1740 type of the National Uniformed Long-Term Fertilization Experiments
at Karcag.
Close correlation (r=0.95) was found between the 0.01 M CaCl2 and ammonium lactate - acetic acid (AL) extractable K contents of
soils.
K fertilization increased the amount of 0.01 M CaCl2 and AL extractable K significantly. Liming had different effects on the amounts of
K extracted by these two methods. Liming increased the amount of AL-K and decreased the amount of CaCl2-K. CaCl2 extractable K was in
close correlation with the relative amount of exchangeable K content of the soil (K%) and the agronomic K balance. The results of regression
analysis confirmed that the CaCl2-K characterized K% and the AL-K related to the absolute amount of exchangeable K.
On the basis of the presented results it can be stated that the 0.01 M CaCl2 is able to detect not just the increase of easily extractable K
caused by fertilization and liming but the changing of the rate of the relative amount of exchangeable K. -
White rust species (Chromista, Peronosporomycetes, Albuginales, Albuginaceae) on common weeds in Hungary
30-33Views:498The obligate plant parasite fungi in the family Albuginaceae are responsible for causing white rust diseases on weeds and they are rather common worldwide. Weedy plants with characteristic symptoms have been collected in 2014 and 2015 on location Hajdú-Bihar and Jász-Nagykun-Szolnok counties in Hungary. The determination of the species were based on the morphological characters both pathogens and hosts. Albugo candida was determined on shepherd’s purse (Capsella bursa-pastoris). Common purslane (Portulaca oleracea) is a host for Wilsoniana portulacae. The fungus Wilsonia bliti (syn.: Albugo bliti), the causal agent of white rust disease was found on redroot pigweed (Amaranthus retroflexus).
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Comparative analysis of certain soil microbiological characteristics of the carbon cycle
137-141Views:310In our researches, we examine the soil microbial parameters related to the carbon cycle. In this study, we compare the changes of microbial biomass carbon (MBC) and the soil CO2 production in soil samples which were taken in spring and autumn. The 30 years old long-term experiment of Debrecen-Látókép is continued in our experiments. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the soil respiration and microbial biomass carbon under non-irrigated and irrigated conditions in maize mono, bi, and triculture.
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Yield components of hairy vetch (Vicia villosa Roth.) in different sowing technologies on acidic sandy soil
83-88Views:850Nearly a quarter of the agricultural utilized area of our country is made up of sandy soils. Sandy soils are poor in nutrients, and, therefore, the effectiveness of farming is basically determined by the method of maintaining soil fertility and the fertilization practice.
The hairy vetch called Vicia villosa Roth (Sandy Roth.), also known as a sand pioneer, plays a significant role in the exploitation of sandy soils. Its cultivation was started in Hungary in the late 1800s. It is primarily used as green fodder, most recently as a green manure and as a soil protection plant. The lupine is grown mainly as a supportive plant, which was previously rye, and today it is triticale. The ratio of the two plants to each other and the spatial location of plants depend on the method of sowing.
The aim of our work was to present the yields of some of the grain grown in different sowing methods and some of its crops.
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Qualitative detection of genetically modified organisms in plant samples
309-313Views:625We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.