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Plant clinic in Nepal: An overview
5-10Views:403Plant clinics play an important role in supporting farmers in growing healthy crops and achieving higher productivity in Nepal. The development and operation of plant clinics in Nepal are assessed in this study through a comprehensive analysis of the institution via literature review and interaction with key stakeholders. The plant clinic approach of the agriculture extension system started in Nepal in 2008, followed up with engagement with CABI and the Government of Nepal. Enhancement of farmers' knowledge and skill, encouraging sustainable farming methods, and ultimately increasing crop yields are the impact of plant clinics. However, there are some challenges faced during the implementation of plant clinics in the existing agricultural extension systems. In Nepal, the plant clinics are primarily operated by agricultural technicians with expertise in plant protection, as well as IPM farmer facilitators and community business facilitators after attending an intensive plant doctor's training. Plant clinics have been integrated into the agricultural extension system by agro-advisory service provider of the government of Nepal. Despite institutionalisation, policy support needs to be strengthened to ensure the sustainability of the different components of the (e.g., data management, validation, monitoring, localised content, etc.) plant clinic in Nepal.
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Insect base-protein: A new opportunity in animal nutrition
129-138Views:907The consumption of insect protein, as well as its use in animal feeding, has become a trend. This trend is particularly receiving a lot of attention in animal nutrition because the current protein source of the highest quality in animal feeding which is fishmeal is unsustainable, expensive and its demand is higher than supply. Insect protein can be of great potential in combating world hunger as the world population continues to increase. The potential of insect protein is wide-ranging, it could improve the economics of feed production, lead to cleaner production due to its low environmental footprint.
This review focuses on the need, (nutritional and environmental) advantages of insect protein in feed production, as well as previous research findings.
The unanimous conclusion of the reviewed papers is that insect protein has a great potential in feed production in terms of nutritional value, environmental implication, sustainability as well as economic implication.
However, there is a need for the intensification of research on this alternative protein source in Europe, especially in aquaculture due to the approval of 7 insect species in aquafeeds by the European Union. Another reason for the intensification is that there is still much to be done as a result of past research which shows that growth effects vary based on species and inclusion level.
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Results of foliar treatments on Sugar beet (Beta vulgaris L.)
303-308Views:346The yield and quality of the sugar beet are mainly determined by the plant production system, thus we studied the effect of mineral fertilization. Our field trials were carried out in 2005 and 2006 in Hajdúböszörmény, at two sites. We studied the effect of sulphur (Cosavet DF), copper (Kelcare Cu) and two foliar nutrients with high active agents. Treatments were replicated four times.
We took root samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfa-amino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments in both of the two years. -
Change of mineral and organic nitrogen forms in a long term fertilization experiment (literature)
43-47Views:301The research topic has timeliness, since the rational utilization and protection of the soil, besides the conservation of its diverse functions is part of the sustainable development. Research of the long-term experiments is esentially important, because it can model the term effects in the same place, under the same conditions. If we want to get accurate informations about the occured changes, way and danger of changes, we should track the resupply and effect of the mineral nutrients and the removed quantity of nutrients with the harvest. Nitrogen is an essential element for living organisms, it is present in the soil mainly in organic form. In general only only a low percentage of the total nitrogent content can be used directly by plants in the soil. This inorganic nitrogen is produced by the transformation of organic contents through mineralization processes and it get into the soil by the fertilization. The plants incorporote the mineral nitrogen into our bodies. This is how nitrogen turnover is realized when mineral forms become organic and organic forms become mineral.
The purpose of our paper is to make a literature before our research.
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Evaluation of chickpea (Cicer arietinum L.) in response to salinity stress
105-110Views:810Soil salinity is a severe and expanding soil degradation problem that affects 80 million ha of arable lands globally. Chickpea (Cicer arietinum L.) is very sensitive to saline conditions; the most susceptible genotypes may die in just 25 mM NaCl in hydroponics. Approximately 8–10% yield loss in chickpea production is estimated due to salinity stress. However, it is still not established why chickpea is so susceptible to salt affection. Salinity (NaCl) impedes germination of seeds, though chickpea varieties considerably differ from one another in this respect. Some chickpea genotypes are more tolerant in the stage of germination, tolerating even 320 mM NaCl. The reasons of this variation are unrevealed; there is a shortage of knowledge about the germination abilities of chickpea genotypes in saline conditions. Nevertheless, the effect of salt stress on vegetative growth can be analysed in hydroponics, in pot or field conditions, regardless the experimental environment, the ranking of genotypes regarding salt resistance is coherent. Chickpea genotypes can be different in their ability to retain water, maybe under salt affection; the more salt tolerant lines can maintain higher water content in the shoots, while the more sensitive ones cannot. The identification of salt tolerant chickpea landraces based on developing genetic variability is a suitable strategy to combat against salinity problems arising in arid and semi-arid areas.
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The main influencing factors effecting the yield of maize
137-141Views:328Maize is one of Hungary’s major cereals. In the 1970s and 1980s, we were in the frontline regarding yields and genetic advancement. However, yield fluctuation in maize has increased to 50-60% from 10-20% since the 1980s, which was partly caused by the increase in weather extremes due to climate change and by agrotechnical shortcomings.
The experiments were carried out on typical meadow soil in four repetitions in the period of 2007-2008. In the sowing time experiment, sowing was performed on 10 April, 25 April, 15 May under a uniform fertilization of N120, P2O580 K2O 110 kg/ha. In the fertilization experiment, the yielding capacity of 10 hybrids with different genetic characteristics was studied in a control (non-fertilized) treatment and basic treatment of N40 P2O5 25, K2O 30 kg ha-1 active ingredient and a treatment with fivefold dosages of the basic treatment. In the plant density experiment, the relationship between plant density and yield was analysed at plant densities of 45, 60 and 75 thousand plants per ha. We found a tight correlation between sowing time and yield and grain moisture content at harvest. We found that grain moisture can be reduced by 5-10% by applying an earlier sowing time.
The agroecological optimum fertilizer dosage was N 40-120, P2O5 25-75, K2O 30-90 kg ha-1 active ingredient at a plant density of 60-90 thousand plants ha-1 depending on the hybrid and the year. -
Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:576In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. -
Evaluation of various silo sorghum hybrids from the energetic aspect
107-111Views:257The sweet sorghum is a perspective plant of bioenergy, which can be the foundation of Hungarian bioethanol production in the future. By the examination six sweet sorghum hybrids have been examinated by the viewpoint of sugar aggregation and bioethanol production capacity. The founda tion of the surve y was three growing se ason (201 0, 2011, 2012 ). The ex per ime nt w as on the production sites of U niversity of Debrecen CAAES RISF Karcag Research Institute. The production site of the sweet sorghum have been sampled by samples of 1 m2 by hybrids within the period of august 15 and november 15. The sugar content of the samples have been measured by refractometer which was the base in the determination of ethanol production capacity.
As a continuation of the evaluation of energetic viewpoint the Higher Heating Value (HHV) have been measured from the bagasse of sweet sorghums. According to the results it can be stated that int he case of sugarcontent and the dinamics of the sugar aggregation can show several differences, while HHV of sorghum bagasse is within 16 200 and 16 900 J g-1.
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Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)
39-43Views:306Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital and essential aliphatic amines which are also present in plants. Although ethylene and polyamines are also involved in fruit ripening, the genes coding them must also take part in other biosynthetic pathways. In the ethylene and polyamines play an important role in development of salt stress tolerance, and in responses for biotic and abiotic stresses. Exogenous application of all three main polyamines (Put, Spd, Spm) increase salt tolerance of plants, but, accordingly to previous experiments, spermidine has the main effect on the enhancement of salt tolerance. Nicotiana tabacum L. plants were grown in vitro on MS medium, the treatments were as follows: arginine (150 mg l-1), putrescine (10 mg l-1), spermidine (10
mg l-1). Proline, chlorophyll a, b and polyamine contents were measured. The obtained results show that the arginine decarboxylase and the spermidine synthase genes involved in polyamine metabolism, cannot be enhanced by exogenous addition of their precursor molecules. On the contrary, the spermine synthase gene has a positive effect to the lower-class forms of polyamines. -
The Role and Significance of Soil Analyses in Plant Nutrition and Environmental Protection
3-8Views:268Hungary has a rich history of soil analyses and soil mapping. Our main tasks today are the preservation of soil fertility as well as balancing the goals of production and environmental protection. The main requirement of agricultural production is to adapt to ecological and economic conditions.
In a series of consultative meetings in the past seven years, representatives from Central and Eastern Europe have analyzed nutrient management practices in their respective countries. According to a joint memorandum agreed upon in 2000, in the countries awaiting accession, the quantity of nutrients used per hectare is considerably smaller than the Western-European usage targeted through special subsidies. The current low nutrient usage contradicts the principles of sustainability and that of the efficient use of resources, jeopardizing soil fertility.
In Hungary, the use of inorganic fertilizers underwent a dynamic development, which manifested itself in an almost tenfold usage growth between 1960 and 1985. This growth slowed down somewhat between 1985 and 1990 and then reduced dramatically after 1990, reaching record lows at the usage levels of the 60s. The nutrient supply has had a negative balance for the last 15 years.
The increasing and then decreasing usage trends can equally be detected in the domestic yield averages of wheat and corn as well as in the nutrient supply of soils. Yields were the largest when usage levels were the highest, and decreased thereafter. Draughts have also contributed to smaller yields. The dramatic decrease in the use of inorganic fertilizers when adequate organic fertilizers are lacking endangers our soils’ fertility.
About 50% of soils in Hungary are acidic. Acidity is mostly determined by soil formation, but especially on soils with a low buffering capacity, this acidity may intensify due to inorganic fertilizers. Sustainable agriculture requires the chemical improvement of acidic soils. According to their y1 values, the majority of our acidic soils need to be improved. This chemical soil remediation is required in 15% of the acidic soils, while it’s recommended for another 20% of these soils.
Results of the analyses conducted in the framework of the soil-monitoring system set up in Hungary in 1992 show that in 95% of the analyzed samples, the toxic element content is below the allowable limit. Cultivated areas are not contaminated; toxicity above the legal level was found only in specific high-risk sampling areas: in the vicinity of industry, due to local overload. The basic principle of sustainable agriculture is to preserve soil fertility without undue strain on the environment. The intensity of the production needs to be considered according to the conditions of the site; i.e.; nutrient management needs to be site-specific. It is recommended to differentiate three types of cultivated land in terms of environmental sensitivity: areas with favorable conditions, endangered areas, and protected areas, and then to adopt nutrient management practices accordingly. To meet all the above-mentioned goals is impossible without systematic soil analysis. Tests conducted by the national monitoring system cannot replace regular field measurements. -
Analysis of vitamin E content in pilot dairy products
31-34Views:404Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount. -
Results of the sensory analysis of precision maize production
59-62Views:569This research was carried out in 2018, at the Látókép Experimental Station of the University of Debrecen in a moderately warm and dry production area, on deep humus layered medium-hard calcareous chernozem soil. In the scope of the research, the chlorophyll content of maize (Zea mays L.) was examined under field circumstances by means of local sensory measurements and we were looking for correlation between the obtained values and the amount of yield. Our measurements were carried out with Minolta SPAD-502 and GreenSeeker devices at 3 measurement times (4 leaf stage, 10 leaf stage and silking). It was found that phenological phases had an effect on the obtained SPAD and NDVI values and were in a slightly significant correlation with the yield. The most significant correlation was found between the results obtained during silking and the amount of yield. This may be because the least time has passed between the measurement time and harvest. Results obtained during the 10-leaf stage show excessive values in each case, which can be due to a measurement error. It was found that the phenological phase had an effect on the correlation of SPAD and NDVI values and the amount of yield. As the phenological phase progressed, the correlation between the measured results and yield has increased.
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Failure causes and effect analysis of water supply systems
209-214Views:139The present paper intends to overview the problems of the failure causes and effect analysis of water supply systems. The author’s efforts were channeled to systemizing, classifying and adapting the content to the hydraulics engineering field.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:604The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Evaluation of Origin and Conformation of Excellent Cows with Long Productive Lives
13-17Views:332In the last few decades a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Beside this reproduction biology of the population was affected adversely by the keeping-, and feeding technology. Serviceable lifetime has shortened and service period has lenghtened. Whereas profitable milk production needs cows with longer productive life, larger life-time production. In our work we analysed the productivity-, and reputation parameters of the 200 highest lifetime-productivity cows of Hungary. We found that inheritance has not got important role in the serviceable lifetime. Adaptability and good constitution of Holstein-friesian is proved by the fact that cows with large life-time production are reared in large plants, some of them in large stocks, industrial environment. We found during the analysis of reputation parameters that these prominent cows mostly have deeper body, higher dairy form, higher rear udder, smaller foot angle. We found some weak connection between the milk production and the reputation. Loose correlations between useful material content and some linear traits were found.
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Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
35-40Views:508Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food, seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.
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A growth model to predict body weight and body composition of broilers
17-24Views:504Models predicting the nutrient partitioning and animal performance have been developed for decades. Nowadays, growth models are used in practical animal nutrition, and they have particular importance in precision livestock farming. The aim of the present study was to introduce a broiler model and to provide examples on model application. The model predicts protein and fat deposition as well as the body weight of an individual broiler chicken from digestible nutrient intake over time. Feed intake (FI) and the digestible nutrient content of the feed are inputs as well as some animal factors like: initial BW, feed intake at 1 and 2 kg of BW, precocity and mean protein deposition. The protein and energy metabolism is represented as in the classical nutrient partitioning models. The protein deposition (PD) is driven by digestible amino acid supply and is under “genetic control”, the so-called potential PD limits the actual PD if protein is oversupplied.
The authors discuss how the model can be used to simulate the animal response upon different scenarios. Examples are given to show that the diet might be limiting if some animal trait is changed. Applicability of the model has shown through running the model by using different feed strategies (three- vs five-phase-feeding) and variations with animal factors. In conclusion, growth models are useful tools to support decision making for defining the most suitable feeds used in a broiler farm. The model presented in this paper shows a high sensibility and flexibility to test different scenarios. By challenging the model with different inputs, the animal response in terms of changes in body weight and feed conversion can be understood more by studying the shift in deposition of chemical constituents. The examples provided in the present paper shows the benefit of using mathematical models and their applicability in precision nutrition. It can be concluded that the growth model helps to apply “from desired feed to desired food” concept.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:530The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Antioxidant density of Hungarian kapia and red bell pepper varieties
80-84Views:304There is a lot of evidence that the pepper is one of the most important sources of vitamin C. Albert Szent-Györgyi was the first to extract ascorbic acid from a red bell pepper in the 1930s. Previously people had eaten vitamin C on the long voyage to protect against scurvy. Vitamin C is an important antioxidant, and it is a cell protector today. Vitamin C protects the cardiovascular system against infection. This vitamin decrease blood-pressure, a raises the HDL cholesterol level (good), and inhibits gout.
„Antioxidant density” is a biological value indicator obtained in synthetic way. „Antioxidant density” indicates the antioxidant capacity of a particular food, e. g. fruits and vegetables, related to 1 Calorie. In our study, we measured the total antioxidant capacity, vitamin C content and „antioxidant density” of Hungarian paprika varieties. -
Anaerobe degradation of maize infected by Fusarium graminearum
57-61Views:554Last year intense rainfalls and moisture conditions were beneficial for the Fusarium sp. in Hungary. Fusarium strains decrease cereal quality (for example maize), furthermore may cause yield loss. Due to the toxin production, the fungi have a dangerous animal and human pathogen effect (Placinta et al., 1999).The effects of the Fusarium infection and its mycotoxin production haven’t been perfectly eliminated. Fusariumgraminearum
is the most common agricultural pathogen in Hungary. The utilization of infected maize as an alternative biogas raw material may be an efficient and environmentally friendly disposal method. In this case, Fusarium-, and mycotoxin-content of the maize have to be analyzed as well as the impact of these factors’ on the biogas production process. Our experience was based on the raw material basis of a biogas plant. Different amount of Fusarium free and infected maize grits have been added to the regular raw material mixture. The detection of Fusarium fungi has been analyzed
in experimental digesters throughout the different stages of mesophilic digestion. In the biogas liquid end product the Fusarium was detected by breeding and by microscope. According to our results, the Fusarium sp. was not detectable in the liquid end product after 30 days. -
Effects of agrotechnical factors on the quality and quantity of yield in winter wheat production
69-75Views:441The present study was conducted to determine the effect of basic agrotechnical factors on the yield and quality of winter wheat. Two experiments were set in 2017/2018 growing season, where we studied the influence of different forecrops, fertilizing treatments and cultivars. 204 samples were measured with Single Kernel Characterization System and NIR grain analyser to determine protein (NIR-P), wet gluten (NIR-WG), Hardness Index (HI), kernel weight (KW) and kernel diameter (KD). Fertilizing had a significant effect on yield, KW, HI, NIR-P and NIR-WG, except KD. N90PK dosage was enough to realize yield potential for 6 out of 9 cultivars, but considering protein content N150PK dosage was needed. The forecrop had no significant influence on yield, KW, KD or HI, however sweet corn as previous crop had significant improving effect on NIR-P and NIR-WG compared to sunflower as forecrop. According to our data of correlation analysis, no negative relationship was found between yield and NIR-P, however HI was in medium positive correlation with NIR-P. The variety Vyckor had the highest yield, but in quality aspect, the highest NIR-P and NIR-WG values belonged to KG Kunhalom variety.
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Correlations Between the Crown Sizes, Pruning Times and Fruit Quality of Sour Cherry Cultivars
90-94Views:270To increase the intensiveness of sour cherry production, i.e. the use of smaller trees, it is necessary to adopt growth-moderating techniques rather than using dwarfing rootstocks. Apart from the traditional technique of using rootstocks that support stronger growth, new or rarely-used techniques and methods must be adopted. Pruning should be carried out in the summer rather than in the winter, the optimal period being 1-3 weeks after harvest.
A combination of the increase in favourable exposure-time and smaller crown sizes make higher tree densities (tree/ha) and better utilisation of the crown (specific cropload) possible.
The systematic use of summer pruning leads to better quality fruit. -
The importance of selenium in the living world and in the scientific research
278-286Views:724Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Effect of agrotechnical factors on the activity of urease enzyme in a long term fertlization experiment
43-48Views:392The soil is a natural resource, the fertility preservation is an important part of the sustainable development. We have to monitor the transformation dinamics of the organic nitrogen-containing substances, to get accurate information about the changes of the nitrogen cycle in the soil.
Physical and chemical properties of the soil and the microorganism effect on the organic matter in the soil – in addition to the composition of organic matter. Wide variety of extracellular enzymes are present in this decomposition. These enzymes help in the transformation of the macromolecules to transforming low molecular weight compounds so they will be available during the assimilation.
The urease enzyme, catalyzes the hydrolysis of urea to CO2 and NH3. The urease is widely spread in the nature, it is present in the microorganisms, plants and animals.
We found that the soil moisture content, the rotation and the fertilization affect to the amount of urease in spring. Furthermore, we get significant difference between the irrigated and non irrigated samples in the second period of the year. Based on our results we can state that the activity of urease was higher in spring 2014.
The objective of our study was to present how the different agronomic factors affect on the activity of urease in a long term fertilizationexperiment.
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Cytokinin and auxin levels in micropropagating Red Fuji and McIntosh apple varieties
53-59Views:216Effects of media hormone content on in vitro shoot multiplication and rooting were examined in cv. Red Fuji and McIntosh apple scions. Multiplication responses of shoots to different concentrations (0.5 and 1.0 mg/l) of 6-benzylaminopurine and 6-benzylaminopurine riboside were tested at two levels (0.1 and 0.3 mg/l) of indole-3-butyric acid. The best proliferation rate was achieved on medium containing 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l indole-3-butyric acid in cv. Red Fuji (5.3) and on medium containing 1.0 mg/l 6-benzylaminopurine and 0.3 mg/l
indole-3-butyric acid in cv McIntosh (10.3). The length of shoots on these media was enough for rooting (38.4 mm in cv. Red Fuji and 39.3 mm in cv McIntosh). Shoots developed on the best proliferation medium were used for rooting. Effects of different concentrations of auxin in the root induction media and presence of activated charcoal in the root elongation media were examined on rooting capacity. The best rooting rate (100% in cv. McIntosh and 83% in cv. Red Fuji) was achieved when the root induction medium contained 1.0 mg/l indole-3-butyric acid. In general, rooting was inhibited in the presence of activated charcoal. Because of high in vitro multiplication and rooting rate and high percent of survival during acclimatisation, the methods described here make effective micropropagation processes possible for cv. Red Fuji and McIntosh.