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Inland and foreign Erwinia amylovora isolates by carbohydrate utilization
29-33Views:89Fire blight, a plant disease caused by the bacterium Erwinia amylovora, produces serious losses in apple and pear orchards all over the world. Since the appearance of fire blight in Hungary (Hevesi, 1996) Erwinia amylovora isolates were collected in different years, from different hosts and areas in order to establish gene bank for future epidemiological studies. We had isolates from foreign countries as well. The aim of our research was to compare all of the Hungarian and foreign isolates by carbohydrate utilization.
In our experiments effect of carbohydrates on E. amylovora multiplication was determined using API 50 CH strip (bioMérieux, France). By the API 50 CH strip method we checked a number of unstudied carbohydrates. The results of the tests shows colour changes. Based on utilization of 49 carbohydrates of API 50 CH kit by E. amylovora isolates, two groups of carbohydrates can be defined: “Utilized” - and “Not utilized” carbohydrates. All isolates utilized 20 different carbohydrates after 164 hour incubation. Conversely, isolates also could be divided into four groups (1, 2, 3, 4) by arbutin and raffinose utilization. In group 1.-isolates utilize arbutin; 2.- utilize raffinose; 3.- utilize both arbutin and raffinose; 4. -utilize neither arbutin nor raffinose. Presumably carbohydrate content of nectar could play an important role on invasion of the (E. amylovora) bacterium via flower.
It could be concluded that the carbohydrate utilization – completed with genetic analysis – can be used for characterization of Erwinia amylovora isolates. -
Optimization of inductively coupled plasma mass spectrometer parameter’s to measuring arsenic and selenium
59-64Views:218In the last decades an increased interest has been evolved about arsenic and selenium. The aim is to understand the environmental, agricultural and biological role of the these elements. In case of arsenic the mayor reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking waterbases of some Asian countries besides Hungary. The toxicity of higher level selenium content is also known, nevertheless selenium is essential for some biological functions. Considering its esssentiality, in our country the insufficient selenium intake rate couse lack of selenium. Measuring the concentrations of these elements are cruital but not satisfactory information, but the speciation, that is the form of an element presented in a sample is also required.
In both cases the most suitable method to determine concentration is the inductively coupled plasma mass spectrimetry. My objective was to optimase the changeable parameters of the ICP-MS for reaching the lowest (the best) detection limit. For this porpuse I have investigated the effect of parameter change on nett signal intensity and relative signal intensity. With the optimased parameter settings the limit of detection for arsenic and selenium were determined, which are 0,032 ng cm-3 for arsenic, and 0,097 ng cm-3 for
selenium. -
Results of the sensory analysis of precision maize production
59-62Views:232This research was carried out in 2018, at the Látókép Experimental Station of the University of Debrecen in a moderately warm and dry production area, on deep humus layered medium-hard calcareous chernozem soil. In the scope of the research, the chlorophyll content of maize (Zea mays L.) was examined under field circumstances by means of local sensory measurements and we were looking for correlation between the obtained values and the amount of yield. Our measurements were carried out with Minolta SPAD-502 and GreenSeeker devices at 3 measurement times (4 leaf stage, 10 leaf stage and silking). It was found that phenological phases had an effect on the obtained SPAD and NDVI values and were in a slightly significant correlation with the yield. The most significant correlation was found between the results obtained during silking and the amount of yield. This may be because the least time has passed between the measurement time and harvest. Results obtained during the 10-leaf stage show excessive values in each case, which can be due to a measurement error. It was found that the phenological phase had an effect on the correlation of SPAD and NDVI values and the amount of yield. As the phenological phase progressed, the correlation between the measured results and yield has increased.
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A growth model to predict body weight and body composition of broilers
17-24Views:355Models predicting the nutrient partitioning and animal performance have been developed for decades. Nowadays, growth models are used in practical animal nutrition, and they have particular importance in precision livestock farming. The aim of the present study was to introduce a broiler model and to provide examples on model application. The model predicts protein and fat deposition as well as the body weight of an individual broiler chicken from digestible nutrient intake over time. Feed intake (FI) and the digestible nutrient content of the feed are inputs as well as some animal factors like: initial BW, feed intake at 1 and 2 kg of BW, precocity and mean protein deposition. The protein and energy metabolism is represented as in the classical nutrient partitioning models. The protein deposition (PD) is driven by digestible amino acid supply and is under “genetic control”, the so-called potential PD limits the actual PD if protein is oversupplied.
The authors discuss how the model can be used to simulate the animal response upon different scenarios. Examples are given to show that the diet might be limiting if some animal trait is changed. Applicability of the model has shown through running the model by using different feed strategies (three- vs five-phase-feeding) and variations with animal factors. In conclusion, growth models are useful tools to support decision making for defining the most suitable feeds used in a broiler farm. The model presented in this paper shows a high sensibility and flexibility to test different scenarios. By challenging the model with different inputs, the animal response in terms of changes in body weight and feed conversion can be understood more by studying the shift in deposition of chemical constituents. The examples provided in the present paper shows the benefit of using mathematical models and their applicability in precision nutrition. It can be concluded that the growth model helps to apply “from desired feed to desired food” concept.
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The analysis of ostrich chick vitality
35-40Views:134Examinations on ostrich chick vitality can help to improve the effectiveness of Hungarian ostrich husbandry and hatching technology. The investigations were carried out on an ostrich breeder farm in Eastern Hungary. For the analyses, the Tona et al. (2003) scoring system was applied presenting eight main criteria (activity, feather, condition, eyesight, the condition of navel and leg, the amount of the remaining shell membrane and egg content, and the size and tenderness of the abdomen). Most chicks (98.08%) were vigilant and had clean and dry feathers. There was no difference between the average hatching weights between May and August but a decreasing tendency was shown during the laying season. The vitality total score was above 90 in each evaluated month and was not affected by the hatching month. Our results revealed that the vitality of chicks was not affected by either the hatching month (P=0.51) or the weight category (P=0.11). Neither the hatching weight of chicks and leg condition were correlated (P=0.79). Results showed that the Tona scoring system with small modifications can be well applied to ostrich chicks. Practical on-farm usage of the system can be suggested as an aid in day-old chick evaluation. After individual marking of chicks, the investigation of growth and culling rate could be carried out for more precise conclusions, involving more farms and laying seasons.
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Measurement of the degradation of abandoned turf
145-149Views:197With the decreasing number of grazing livestock in Hungary, the role of the turf cultivation is also significantly decreasing. The proportion of the under- and non-utilized turf is increasing. In the research conduced at the University of Debrecen, IAREF Research Institute Karcag, we studied four types of turf utilization in three replicates on a salt field with timothy grass. We determined the flora composition of the experimental area, measured the soil moisture and the carbon-dioxide content of the soil.
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Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
35-40Views:211Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food, seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.
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Effect of the dry and rainy weather on the Idared and Golden Reinders apple varieties fruit quality
107-110Views:118Weather conditions have an important role in fruit production. In the last few decades, this role is increased and basically determines the
fruit quality and quantity. Despite of this statement, there is but very few information about impacts caused by weather anomalies in
Hungarian orchards. Regarding this, the relation between the external, and internal values of the Idared and Golden Reinders apple varieties,
the changing of the temperature and precipitation in a dry year (2009) as well as in a rainy year (2010) were investigated. The examined
apple varieties are grown at the same training system (rootstock, spacing, training, pruning system). The average weight, average diameter,
acid content and soluble solids were higher in 2009 than in 2010, although the precipitation was higher in 2010. Due to the many rainy days
the intensity of sunshine and the number of sunny days were less. To produce better quality it is very important the amount of the sunshine
and heat, the optimal temperature and the precipitation rate. -
Establishing regional cultivating districts on the basis of the Kreybig practical soil mapping system
20-25Views:116With the help of this report evaluating the current situation of the region, characteristics of the development in agricultural production and regional differences can be clarified. By mapping out the regional soil, land use and climatic conditions and organizing these into a geographical information system, one can easily determine which plants are the most ideal to cultivate in that particular region. Moreover, it is a useful tool that enables us to
establish the most favorable land use structure suited to ecological demands and also helps to determine the methods of soil protection.
During our work, we chose administrative units in Szabolcs-Szatmár-Bereg County, based on the latest aspects of regional cultivation.
Our pilot areas are: the small regions of Nyíregyháza, Nyírbátor, Nagykálló, Mátészalka and Csenger.
Using the database, we separated and uncovered the soil conditions of the pilot areas: the chemical and physical properties of the soil layer which is exploited by the roots of the plants, the humus content, the nutrient supply, the thickness of the cultivated layer and the water management conditions.
We separated the districts of regional cultivation, where the basic elements of the traditional Kreybig color systems were applied (light yellow, dark yellow, light brown, dark green, blue, pink, red, gray, greenish brown, reddish purple, light purple, dark purple, light green).
By using the data collected from the pilot areas, we compiled a map database, which is suitable to illustrate the plant cultivating characteristics of the region. We made recommendations to determine the most favorable plants to cultivate in the specific region with the given meteorological and soil conditions, as well as for the shifting of crops.
Our recommendations were also illustrated in a map with a resolution of 1:25000. -
The Effect of Changes in Forest Area on the Transcarpathian Tisza River Basin
181-185Views:83Forests are unique global factors which ensure life for almost every living being on Earth. They play a major role in controlling water flows, preventing erosion and controlling the oxygen content of Earth’s atmosphere. By the end of the XXth century, it was realized that forests help to nature maintain and are vital parts of our natural environment. By the time societies realized this fact, economic and environmental effects had amplified which endanger forests. Due to their good water control and water protection abilities, and their function in climate control, mountain forests can provide a suitable environment for themselves, for their successful growth.
Forests play a major role in soil protection, especially in mountain areas where they prevent soil erosion. By converting surface waters into subsurface waters, forests help with the accumulation of subsurface waters, which are the sources of springs, rivers and streams. In the summer, they protect the soil from drying out by creating a special microclimate. They positively affect the climate of surrounding territories. -
The influence of fertilization on the soil characteristics of a calcareous chernozem in a long term experiment
47-52Views:95In the long term fertilization experiment of the University of Debrecen, Centre for Agricultural and Applied Economic Science(CAAEC) (Debrecen Látókép), the effects of a 25-year-long fertilization were examined in terms of some chemical and microbiological properties of soil. With the growing doses of fertilizers, the available nutrient content of soil increased. At the same time the pH significantly decreased, while the hidden acidity increased. Moreover, the ratio between the soil bacteria and microscopic fungi, and the occurrence of microbes also changed. The number of sensitive physiological bacteria groups decreased dramatically. These changes indicate the reactions of living organisms; they correspond to the „resistance stage” of stress effects, but in the case of nitrifying bacteria, they reach the „exhaustion stage”.
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Prospects of social welfare work in Hungary and in Hajdú-Bihar county
127-130Views:131The social care has been accompanying phenomenon of mankind from ancient times. An individual's social and economic processes that affect the lives basically forced the representatives of the powers to help those in need. The concepts used in social care today (equality, solidarity, etc.) have evolved over the centuries, shaped by political ideologies of the content. This wide range of people living in today's society for granted the fact that life is present in the social care system, which is necessary for security.
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The Role of Cultivation Systems in Quality Sugar Beet Production
134-138Views:131Producing sugar beet, as it is a demanding field crop, has contributed to the raising standard of plant production. It has an outstanding place among the plants that are cultivated in the intensive plant production system. Rentability of sugar manufacture is determined by the stability of yield and the quality (saccharose content) of sugar beet. In this way, the fundamental interest both of the producers and the processing industry is high yield and quality, year by year. The yield and the quality of the sugar beet are mainly determined by the plant production system, so we studied the effect of fertilization, irrigation and plant protection.
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Effect of agrotechnical factors on the activity of urease enzyme in a long term fertlization experiment
43-48Views:150The soil is a natural resource, the fertility preservation is an important part of the sustainable development. We have to monitor the transformation dinamics of the organic nitrogen-containing substances, to get accurate information about the changes of the nitrogen cycle in the soil.
Physical and chemical properties of the soil and the microorganism effect on the organic matter in the soil – in addition to the composition of organic matter. Wide variety of extracellular enzymes are present in this decomposition. These enzymes help in the transformation of the macromolecules to transforming low molecular weight compounds so they will be available during the assimilation.
The urease enzyme, catalyzes the hydrolysis of urea to CO2 and NH3. The urease is widely spread in the nature, it is present in the microorganisms, plants and animals.
We found that the soil moisture content, the rotation and the fertilization affect to the amount of urease in spring. Furthermore, we get significant difference between the irrigated and non irrigated samples in the second period of the year. Based on our results we can state that the activity of urease was higher in spring 2014.
The objective of our study was to present how the different agronomic factors affect on the activity of urease in a long term fertilizationexperiment.
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Monitoring the oxygen level in the Szarvas-Kákafok Deadarm
170-173Views:86The water quality of the Szarvas-Békésszentandrás Dead Körös is generally meso-eutrophic, and meso-saprobic. However, particularly
under higher temperature conditions, the water body may change toward the eutrophic state, even algal blooms could be observed
previously.
The present measurements were conducted during a two week period, twice a day. Three water samples were taken horizontally, from
the surface, bottom and the middle of the water body. The samples were examined in situ. The oxygen content, the temperature, the pH and
the conductivity were measured by potentiometric methods.
Increasing pH was detected in correlation to the temperature, which indicated a rising photosynthetic activity. Also, the O2
concentration showed high variations, especially, when the fresh water supply from the river was stopped, due to a small flood in the river
Körös.
These results indicate the increasing eutrophication processes in the deadarm, and the high load and instability of the ecosystem. -
Effect of fish feeds with high vegetable oil and low fish oil content on the growth performance and innate immune system of common carp (preliminary results)
7-12Views:100Fish meal and fish oil are very important components of fish feeds used by aquaculture. They contain poly-unsaturated fatty acids, which are essential nutrients both for fish and human nutrition. However, sources of fish meal and fish oil are limited, there is a need for new feed components to supply the increasing fish production and the increasing demand for fish feed. The aim of AQUAMAX project, supported by the European Union is to replace the fish meal and fish oil with vegetable oils. As a participant of this project, group of immunology in Research
Institute for Fisheries, Aquaculture and Irrigation (HAKI) examined the effect of three different fish feeds on the growth performance, innate immune response and resistance against Aeromonas hydrophila infection of common carp in an in vivo experiment. Fish feed supplemented with Camelina oil, but still containing fish oil significantly increased growth performance of experimental fish and their resistance against the infection. Fish feed containing linseed oil only did not enhance the growth performance, and it had a negative effect on resistance against infection. Based on the experimental results it can be concluded that fish oil used in fish feeds cannot be replaced effectively with vegetable oils, but can be supplemented with them. -
Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:185In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. -
Study of some physiologic properties of different genotype sweet corn hybrids
105-110Views:115The effect of nutrient-supply (control, N120+PK) and two different genotypes on the physiologic properties of sweet corn has been investigated in the crop-year of 2011 on chernozem soil in the Hajdúság region. The experiments were carried out at the Experimental Station of the University of Debrecen in Debrecen-Látókép. The experiment was sewn in two different sowing times: the 21st April can be considered as an early, while the 19th May as a late sowing time. The two involved hybrids were Jumbo and Enterprise. The applied plant density was 65 000 plants per hectare.
Our aim with this experiment was to study the plant production, just as the main affecting factors of its development and dynamics, like nutrient-supply and genotypes. We aimed to study and analyse the relationships between these factors and plant production. In this study following parameters were measured and calculated: photosynthetic activity, chlorophyll-content (SPAD-value), leaf area index (LAI) and leaf area duration (LAD). Regarding the analysis of photosynthetic activity values no obvious relationship between the measured values and the applied hybrids, just as nutrient-supply has been revealed.
Analysing the SPAD-values it can be stated that the chlorophyll-content of the measured leaves showed an increasing tendency due to the nutrient-supply. The highest values have been measured in the intensive cob development phase of the early sowing time plots.
Regarding the LAI-values we have found significant differences between the fertilizer treatments in both sowing time treatments. In case of the leaf area duration values – that is derived from the LAI values – nutrient-supply has positively affected the duration of the assimilation area. -
Agronomical and economic evaluation of various cultivation systems on meadow soil
103-106Views:122The requirements and objectives of cultivation are in constant change. There are different cultivation aims if the objective is soil protection, the prevention of its moisture content or on areas with different precipitation supply or production site endowments. Based on the experimental database of the Institute for Land Utilisation, Regional Development and Technology of the University of Debrecen, Centre for Agricultural and Applied Economic Sciences and the KITE Plc., the various cultivation systems in Hajdú-Bihar country were examined with maize as indicator plant. The sample area can be found in the outskirts of Biharnagybajom on meadow soil. On the examined plot, spring strip basic cultivation, loosening and autumn ploughing were applied on 15-15-15 ha, respectively. At the time of taking undisturbed soil samples, soil conductivity measurements were also performed with a Penetronik penetrometer. Undisturbed soil samples were taken from each treatment before sowing (on 5th April 2012). The yield obtained in the strip cultivation treatment increased that of the ploughing and the loosening technology. The economic indexes are the most favourable in the strip cultivation.
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The relationship between the nutrient supply and the yield of maize hybrids with different genetic traits on chernozem soil in variant years
27-31Views:210The experiments were set on lime-coated chernozem soil in 2013 and in 2014, in our study four hybrids were included with different FAO number. We studied the effect of NPK fertilization and row spacing on the yield. The fertilizer doses were based on a 25-year longterm experiment. Compared to control, the N40 +PK treatment has also achieved a significant yield increase, although some hybrids responsed with yield loss to the increasing fertilizer doses; this effect was observed especially in 2014. The majority of hybrids reached higher yields in both years using the 50 cm row spacing. The water release of hybrids was measured weekly during the maturation, at the same time points. The rainy September slowed ripening and the water release of the hybrids in 2013, so the grain wet content at harvest showed higher values. The moisture contents were increased for some hybrids, in spite of the positive and favorable dynamic of water loss.
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Genetic progress in winter wheat quality and quantity parameters
71-75Views:173Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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A földhasználat alakulása az ezredfordulón - egy felmérés eredményei
58-66Views:99There were no significant changes in land ownership and use in 2000, compared to the period following the compensation process. Land is mainly owned by people who do not want to use their property, so they generally turn to renting it out to others. Although farming on rented land is performed under various conditions, farms try to rent the lands of better quality. In general, examined farms would like to increase their size by tenancy or land purchase. Buying land is a good investment, considering the price increase in the future, but because of the lack of capital, tenancy remains the main form of territory increase.
Farms which rent land are in connection with a lot of owners, the land within their use is frittered away, it is in a lot of parts. Most rental contracts are written, but the ratio of oral contracts is still quite high. To reduce this ratio is one future aim. One more characteristic of the rental contracts is the dominance of a medium length period – which is acceptable for both the owner and user, but the ratio of short time contracts is still high. Land rent, on average for the examined farms, is quite balanced, it is on the level of about 16.000 HUF/hectare.
The land users mainly pay the same land rent to the owners, but in some cases, there are exceptions. Generally, land rent is a fix cost, but sometimes this cost depends on the level of the yield or the change of product price. Land rent, on average, is 16% within the production costs on the examined farms, which try to choose better and larger lands for rent. Sometimes, farmers can pay higher land rent for the unit of better land, but this is not a general tendency. It is mainly true that they pay the same land rent for the unit, regardless of land quality. Land owners can not interfere in the use of their land within the rental period, and also is a main characteristic of contracts that important particulars are not spelled out sufficiently by the partners. It follows that their content has to be improved in the future. -
The significance of ecolabels in tourism
118-124Views:104More than one hundred types of ecolabels have been introduced worldwide since the 1980s. These voluntary certifications may play a prominent role in achieving sustainable tourism as, on the one hand, they may encourage companies to behave responsibly, and on the other, they may contribute to increasing tourists’ awareness in their choice. Considering that there are many small and medium-sized companies working on the tourism market, developing voluntary certification systems is highly encouraged in the member states of the European Union.
At the same time, there are significant differences among the various ecolabels concerning their content, qualitative criteria, and requirements, which makes it difficult for them to gain acceptance on the tourism market. It is worth mentioning that the sets of criteria belonging to the ecolabels usually do not involve indicators related to the social and cultural impacts of tourism.
In my paper I review the main characteristics of voluntary certifications with special emphasis on the players in tourism ecolabels, furthermore I discuss their advantages and some of the arising problems as well. -
Analysis of vitamin E content in pilot dairy products
31-34Views:139Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount. -
The role and impact of N-Lock (N-stabilizer) to the utilization of N in the main arable crops
51-55Views:236The nitrogen stabilizer called N-Lock can be used primarily with solid and liquid urea, UAN and other liquid nitrogen, slurry and manure. In corn it can be applied incorporated before sowing or with row-cultivator or applied with postemergent timing in tank-mix. In postemergent timing need precipitation for long effect. In oil seed rape and autumn cereals the N-Lock should be applied with liquid nitrogen in tank mix late winter or early spring (February-March). The dose rate is 2.5 l/ha. N-Lock increases the yield of maize, winter oil seed rape, winter wheat and winter barley 5-20 %. The yield increasing can be given the thousand grain weight. In case of high doses of nitrogen it can be observed higher yield. The quality parameter also improved, especially the oil content of winter oil seed rape and protein and gluten contents of winter wheat. The use of N-Lock increases the nitrogen retention of soil and reduces nitrate leaching towards the groundwater and the greenhouse effect gas emissions into the atmosphere. The degradation of the applied nitrogen is slowing down and the plant can uptake more nitrogen in long period. The effect of N-Lock the nitrogen is located in the upper soil layer of 0-30 cm and increasing the ammonium nitrogen form. The product can be mixed with herbicide products in main arable crops.