Effect of dietary arginine on organ weight and feed intake in Japanese quail (Coturnix japonica)
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Accepted 2025-12-12
Published 2026-06-02
Abstract
This study showed the effects of different dietary arginine levels on organ weight and feed intake in growing Japanese quail. Quails were provided with diets containing low, control, or high arginine for a fourteen-day experimental period. Dietary arginine levels had no significant effect on relative liver weight in both sexes, whereas low arginine intake was associated with increased relative brain weight. These findings suggest tissue-specific responses, with the liver exhibiting metabolic resilience and the brain being preferentially maintained under nutrient limitation. Feed intake was reduced under low dietary arginine during the second week, indicating sensitivity to arginine availability at this developmental stage. Variations in feed intake across studies suggest that arginine’s effects are context-dependent, influenced by physiological status, environmental conditions, and baseline diet composition. Overall, our findings highlight the role of dietary arginine in organ weight and feed intake, reflecting both direct effects on organ development and indirect effects through feed intake regulation.
References
- Abdullah, H.M.; Bielke, L.R.; Helmy, Y.A. (2019): Effect of arginine supplementation on growth performance and immunity of broilers: A REVIEW. Journal of Global Innovations in Agricultural and Social Sciences, 7(4), 141–144. https://doi.org/10.22194/JGIASS/7.879
- Adomeh, E.E. (2024): relative organ weight of broiler finisher fed ginger, garlic and mixture of ginger and garlic powder. Nigerian Journal of Animal Production, 917–919. https://doi.org/10.51791/njap.vi.5949
- Alagawany, M.; Elnesr, S.S.; Farag, M.R.; Tiwari, R.; Yatoo, M.I; Karthik, K.; … Dhama, K. (2021): Nutritional significance of amino acids, vitamins and minerals as nutraceuticals in poultry production and health – a comprehensive review. Veterinary Quarterly, 41(1), 1–29. https://doi.org/10.1080/01652176.2020.1857887
- Al-Daraji, H.; Al-Mashadani, A.; Al-Mashadani, W.; Al-Hassani, A.; Mirza, H. (2012): Effect of in ovoinjection with L-arginine on productive and physiological traits of Japanese quail. South African Journal of Animal Science, 42(2). https://doi.org/10.4314/sajas.v42i2.6
- Al-Daraji, H.J.; Salih, A.M. (2012): Effect of Dietary L-Arginine on Productive Performance of Broiler Chickens. Pakistan Journal of Nutrition, 11(3), 252–257. https://doi.org/10.3923/pjn.2012.252.257
- Al-Tamimy, S.; Amen, S.; Shaker, A.; Ameen, Q. (2025): The Effect of Adding Different Levels of L-arginine on the Carcass Traits of Japanese Quail. Egyptian Journal of Veterinary Sciences, 56(1), 235–239. https://doi.org/10.21608/ejvs.2024.270710.1855
- Atakisi, O.; Atakisi, E.; Kart, A. (2009): Effects of dietary zinc and l-arginine supplementation on total antioxidants capacity, lipid peroxidation, nitric oxide, egg weight, and blood biochemical values in Japanese quails. Biological Trace Element Research, 132(1–3), 136–143. https://doi.org/10.1007/s12011-009-8378-x
- Banerjee, S.; Chaturvedi, C.M. (2018). Neuroendocrine mechanism of food intake and energy regulation in Japanese quail under differential simulated photoperiodic conditions: Involvement of hypothalamic neuropeptides, AMPK, insulin and adiponectin receptors. Journal of Photochemistry and Photobiology B: Biology, 185, 10–23. https://doi.org/10.1016/j.jphotobiol.2018.05.020
- Bates, D.; Mächler, M.; Bolker, B.M.; Walker, S.C. (2015): Fitting linear mixed-effects models using lme4. Journal of Statistical Software, 67(1). https://doi.org/10.18637/jss.v067.i01
- Bennett, P.M.; Harvey, P.H. (1985): Brain size, development and metabolism in birds and mammals. Journal of Zoology, 207(4), 491–509. https://doi.org/10.1111/j.1469-7998.1985.tb04946.x
- Bilal, R.M.; Hassan, F.; Farag, M.R.; Nasir, T.A.; Ragni, M.; Mahgoub, H.A.M.; Alagawany, M. (2021): Thermal stress and high stocking densities in poultry farms: Potential effects and mitigation strategies. Journal of Thermal Biology, 99, 102944. https://doi.org/10.1016/j.jtherbio.2021.102944
- de Lima, M.B.; de Sousa, M.B.; Minussi, R.T.; de Carvalho, L.C.; Veras, A.G.; Malheiros, E.B.; da Silva, E.P. (2022): Arginine requirement for egg production in Japanese quail. Poultry Science, 101(6), 101841. https://doi.org/10.1016/j.psj.2022.101841
- Fathima, S.; Al Hakeem, W.G.; Selvaraj, R.K.; Shanmugasundaram, R. (2024): Beyond protein synthesis: the emerging role of arginine in poultry nutrition and host-microbe interactions. Frontiers in Physiology, 14. https://doi.org/10.3389/fphys.2023.1326809
- Fidgett, A.L.; Gardner, L. (2014): Advancing avian nutrition through best feeding practice. International Zoo Yearbook, 48(1), 116–127. https://doi.org/10.1111/izy.12057
- Fristoe, T.S.; Botero, C.A. (2019): Alternative ecological strategies lead to avian brain size bimodality in variable habitats. Nature Communications, 10(1), 3818. https://doi.org/10.1038/s41467-019-11757-x
- Herring, C.M.; Bazer, F.W.; Wu, G. (2021): Amino Acid Nutrition for Optimum Growth, Development, Reproduction, and Health of Zoo Animals. In: Amino Acids in Nutrition and Health (Ed.: Editors: Guoyao Wu (pp. 233–253). https://doi.org/10.1007/978-3-030-54462-1_12
- Iheanacho, G.C.; Iwuji, T.C.; Ogamba, M.C.; Odunfa, O.A. (2022): Relationship between live weight, internal organs, and body part weights of broiler chickens. Malaysian Animal Husbandry Journal, 2(2), 64–66. https://doi.org/10.26480/mahj.02.2022.64.66
- Kalvandi, O.; Sadeghi, A.; Karimi, A. (2022): Arginine supplementation improves reproductive performance, antioxidant status, immunity and maternal antibody transmission in breeder Japanese quail under heat stress conditions. Italian Journal of Animal Science, 21(1), 8–17. https://doi.org/10.1080/1828051X.2021.2013136
- Khajali, F.; Wideman, R.F. (2010): Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World’s Poultry Science Journal, 66(4), 751–766. https://doi.org/10.1017/S0043933910000711
- Kheiri, F.; Landy, N. (2020): Growth Performance, Intestinal Morphology, Serum Biochemical and Hematological Parameters in Japanese quail (Coturnix japonica) Fed Supplemental L-Arginine. Brazilian Journal of Poultry Science, 22(3). https://doi.org/10.1590/1806-9061-2019-1200
- Kidd, M.T.; Maynard, C.W.; Mullenix, G.J. (2021): Progress of amino acid nutrition for diet protein reduction in poultry. Journal of Animal Science and Biotechnology, 12(1), 45. https://doi.org/10.1186/s40104-021-00568-0
- Kumar, A.; Tamta, K., Arya, H.; Arya, S.; Maurya, R.C. (2024): Investigating the impact of nutritional insufficiency on parahippocampal neurons in domestic chickens, Gallus gallus domesticus. Journal of Chemical Neuroanatomy, 137, 102401. https://doi.org/10.1016/j.jchemneu.2024.102401
- Kuznetsova, A.; Brockhoff, P.B.; Christensen, R.H.B. (2017): lmerTest Package: Tests in Linear Mixed Effects Models. Journal of Statistical Software, 82(13), 1–26. https://doi.org/10.18637/JSS.V082.I13
- Lala, A.; Fowowe, A.; Orbugh, A.; Osunsina, I.; Oso, A. (2022): Response of guinea fowls to dietary L-arginine supplementation. Agricultura Tropica et Subtropica, 55(1), 40–48. https://doi.org/10.2478/ats-2022-0005
- Luqman, Z.; Masood, S.; Hameed, S.; Zaneb, H.; Waseem Akhtar, R.; Aftab Hussain Shah, S.; Iqbal, N. (2020): Effect of In-Ovo Administration of L-Arginine on the Gross Anatomy of Tibia Bone, Alkaline Phospahtase and Growth Performance in Japanese Quail (Coturnix japonica). Journal of Animal Health and Production, 9(1). https://doi.org/10.17582/journal.jahp/2021/9.1.22.26
- Morais, V.M.; Lima, J.D.; Silva, N.A.; Gomes, V.F.C. (2022): Performance and egg quality of laying Japanese quails (Coturnix coturnix japonica) reared in hot climate as a function of digestible arginine: lysine ratios in the diet. The Indian Journal of Animal Sciences, 92(10). https://doi.org/10.56093/ijans.v92i10.114248
- Nassar, F.S.; Alaqil, A.A.; El-Sayed, D.A.A.; Kamel, N.N.; Abbas, A.O. (2023): Effects of Dietary Intervention Using Spirulina at Graded Levels on Productive Performance and Physiological Status of Quail Birds Reared under Elevated Temperatures. Agriculture, 13(4), 789. https://doi.org/10.3390/agriculture13040789
- National Research Council (U.S.). Subcommittee on Poultry Nutrition. (1994): Nutrient requirements of poultry. National Academy Press.
- Ndunguru, S.F.; Reda, G.K.; Csernus, B.; Knop, R.; Gulyás, G.; Szabó, C.; Levente, C.; Lendvai, Á.Z. (2024): Embryonic methionine triggers post-natal developmental programming in Japanese quail. Journal of Comparative Physiology B, 194(2), 179–189. https://doi.org/10.1007/s00360-024-01542-8
- R Core Team. (2024): R: A Language and Environment for Statistical Computing. https://doi.org/http://www.R-project.org/
- Reda, G.K.; Ndunguru, S.F.; Csernus, B.; Gulyás, G.; Knop, R.; Szabó, C.; Levente, C.; and Lendvai, Á.Z. (2024): Dietary restriction and life-history trade-offs: insights into mTOR pathway regulation and reproductive investment in Japanese quail. Journal of Experimental Biology, 227(8). https://doi.org/10.1242/jeb.247064
- Reis, R. de S.; Barreto, L. de T.; Abjaude, W. da S.; Dutra, D.R.; Santos, M.; Paula, E. de. (2012): Relationship of arginine with lysine in diets for laying Japanese quails. Revista Brasileira de Zootecnia, 41(1), 106–110. https://doi.org/10.1590/S1516-35982012000100016
- Smaers, J.B.; Dechmann, K.N.; Goswami, A.; Soligo, C.; Safi, K. (2012): Comparative analyses of evolutionary rates reveal different pathways to encephalization in bats, carnivorans, and primates. Proceedings of the National Academy of Sciences, 109(44), 18006–18011. https://doi.org/10.1073/pnas.1212181109
- Sousa, M.; Lima, M.; Vieira, R.B.; Pavanini, J.; Peruzzi, N.J.; Raimundo, E.; Silva, E. (2022): Modeling the response of Japanese quail to arginine intake. PeerJ, 10, e14337. https://doi.org/10.7717/peerj.14337
- Tuba; U.B. (2015): Effects of dietary supplementation of arginine and lysine on performance and egg quality characteristics of laying quails. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 62(4), 307–312. https://doi.org/10.1501/Vetfak_0000002697
- Tuesta, G.; Viana, G.; Barreto, S.; Muniz, J.; Reis, R.; Mencalha, R.; Hannas, M. (2018): Optimal standardized ileal digestible arginine to lysine ratio for Japanese quails in the egg-laying phase. Brazilian Journal of Poultry Science, 20(2), 351–356. https://doi.org/10.1590/1806-9061-2017-0554
- Wu, G. (2009): Amino acids: Metabolism, functions, and nutrition. Amino Acids 37, 1–17. https://doi.org/10.1007/s00726-009-0269-0
- Wu, G.; Bazer, F.W.; Davis, T.A.; Kim, S.W.; Li, P.; Marc Rhoads, J.; Yin, Y. (2009): Arginine metabolism and nutrition in growth, health and disease. Amino Acids, 37(1), 153–168. https://doi.org/10.1007/s00726-008-0210-y
- Zaefarian, F.; Abdollahi, M.R.; Cowieson, A.; Ravindran, V. (2019): Avian Liver: The Forgotten Organ. Animals, 9(2), 63. https://doi.org/10.3390/ani9020063
https://doi.org/10.34101/actaagrar/1/16343