Effect of different treatments to bacterial canker (Clavibacter michiganensis subsp. michiganensis), bacterial speck (Pseudomonas syringae pv. tomato) in tomato, and bacterial spot (Xanthomonas campestris pv. vesicatoria) in pepper
49-53.Vol 13No 22007
In ecological farming systems farmers can't use chemicals against pests. In ecological plant protection the aim is to prevent diseases; if it is not possible the use of allowed materials are permitted. Until now there haven't been enough effective and environmental friendly materials for seed treatment in organic farming. Seed borne diseases of to...mato and pepper can cause serious losses in yield, so finding appropriate inhibitors has a great importance. Different materials were tested against these bacterial strains for seed treatment in this study. In vitro trials have shown that vinegar, cider vinegar, red wine vinegar and white wine vinegar have inhibiting effect against the causative agent of bacterial canker (Clavibacter michiganensis subsp. michiganensis), bacterial speck (Pseudomonas syringae pv. tomato) of tomato. These materials also have inhibiting impact on the causative agent of bacterial spot of pepper (Xanthomonas campestris pv. vesicatoria). Seed treatment with (natural alkaline material) sodium hydrogen carbonate (NaHCO3) had no effect on the examined bacterial strains. Among examined essential oils cinnamon oil seemed to be the most effective, but all oils decreased the germination ability. Thyme and savory teas were effective against Pseudomonas syringae pv. tomato. Other examined materials had insufficient bactericide impact (sucrose, NaCI, ethanol, valerian extract, peppermint tea). The germination test has shown that examined vinegar types don't decrease germination ability.
Changing of carbohydrates by inoculation of Pseudomonas savastanoi pv. phaseolicola oil bean lines with different resistance
82-85.Vol 6No 12000
The Pseudomonas savastanoi pv. phaseolicola (PS) is one of the most significant stressors of bean (Phaseolus vulgaris L.). Chemical and agrotechnical treatments have minor importance, so breeding has great part in the protection against this pathogen. Most of the cultivars are susceptible to PS. The genetic background of resistance in the plant is... a complex system. Leaf resistance is a monogenic system, but there are some modifier genes. The pathogen species can be divided into different races. To understand the functioning of this resistance gene, experiments were carried out using bean varieties with different genotypes and near isogenic lines of bean. Eight lines were tested. Our main objective was to test bean lines with PS with high virulence. The experiment was made in greenhouse and on field. The virulent bacterium strain has been isolated in Hungary. The changes of carbohydrates were tested after infection. In homeostasis the level of carbohydrates (especially glucose and fructose) were higher in susceptible lines. In case of artificial and natural infection the decrease of glucose were more significant in susceptible lines than in resistant lines. In the leaf samples from systemic chlorosis the level of this carbohydrate increased. These changes are connected with the level of resistance, but more experiments are needed to verify this assumption.
59-62.Vol 5No 3-41999
Two strains of Pseudomonas syringae subsp. savastanoi were isolated from Forsythia sp. and Nerium oleander in Hungary in 1997. The effects of growth regulators produced by the bacteria were studied in different experiments. The strains were co-cultured with Sorbus redliana in vitro shoots without being in contact with the plant on solid media. Fur...ther culture filtrates in different concentrations were added to the culture medium. The growth regulators presented in the agar caused knot formation on the shoots and on the leaves in both kinds of culture. There were significant differences in the cultural and physiological characters, auxin and cytokinin activity of the strains of different origin.
The effect of modified bacterial virulence to host-pathogen relationship (Phaseolus vulgaris L. Pseudomonas savastanoi pv. phaseolicola)
53-56.Vol 5No 1-21999
The Pseudomonas savastanoi pv. phaseolicola is one of the most expressive biogen stressors of the bean (Phaseolus vulgaris L.) in Hungary. The chemical and agrotechnological defence is inefficient, so breeding is the only workable way. The conventional cultivars are susceptible to PS while most of the new industrial varieties have genetic resistan...ce to the pathogen. The genetic background of resistance is, however, a complex system in the bean. Leaf resistance is a monogenic system, but this gene is not expressed in juvenile stage of the host. The pathogen species can be divided into different races. After inoculation with virulent strains, typical symptoms appeared on the leaves. To understand the details of host-pathogen relationships, there were carried out experiments using bacterial strains with altered virulence. Six transposon mutants of the PS were tested. Our main objective was to test these modified bacterial strains on bean cultivars of known genetic background. First we analysed the symptoms, and then the correlation between the symptoms and the multiplication of mutant bacteria. Three cultivars (Cherokee, Inka and Főnix) were tested. The infection by the virulent PS isolate produced typical symptoms on the three cultivars tested. Mutant bacteria (except strain 756) did not cause any significant symptoms on the hosts. The mutant 756 induced visible symptoms on the cultivars Cherokee and Inka. On Cherokee there were small watersoaked lesions, and HR (hypersensitivity reaction) was detected on Inka, but this was restricted to some cells only (mikro HR). The rate of multiplication of the wild type strain was much higher than the multiplication of the mutants. Bacteria were detected in the cotyledons and primordial leaf, but there is not any substantial number of bacteria in leaves, except for strains 757, 1212 and 1213. The rate of multiplication of strain 756 was intermediate. These, and other experiments can help to understand the genetic background of resistance and the host-pathogen relationship in the Pseudomonas-bean pathosystem.
49-54.Vol 14No 42008
In organic farming systems the focus is on prevention with regards to plant protection. To follow the rules of Good Agricultural Practice one is able to avoid serious yield losses; if it is not possible the use of allowed materials are permitted. Organic farmers have less material to protect their plants so it is necessary to find effective potent...ial materials. Bacterial and fungal diseases of tomato and pepper can cause serious losses in yield. Different materials were tested against some plant pathogen bacterial (Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria and fungal (Phytoptora infestans, Rhizoctonia solani, Sclerotinia sclerotium) strains in order to find potential materials in the field of organic seed treatment. In vitro trials have shown that vinegar, cider vinegar, red wine vinegar, white wine vinegar, cinnamon and thyme oil have inhibiting effect against the causative agent of bacteria and fungi. Germination test has shown that examined vinegar types do not decrease germination ability if the concentration is low, but in higher (more than 5%) concentration it ruins the germination ability. Even in 0,5% concentration of red — and white vine vinegar have good effect on germination capacity.
Some microbial treatments against the tomato leaf miner; Tuta absoluta (Merick) under natural field conditions
81-84.Vol 19No 1-22013
In an attempt to find more effective methods and safety to control the tomato leaf miner, Tuta absoluta, this research was conducted to study the efficacy of the four bio-treatments ; two bacterial isolates (Bacillus subtilis. and Pseudomonas Fluorescence), one fungal isolate Trichoderma viride) and Spinosad as a microbial insecticide compared wit...h the most common insecticides used against the dipterous and lepidopterous leaf miners in Egypt; Profenofos and Aphox. In the two successive seasons; 2011 and 2012, the fungal isolate Trichoderma viride achieved the lowest infestation(numbers of infested leaflets, mines, infestation percentages) which were 167, 195, and 21.9% resp.in season 2011 and 198, 222 and 26.2% resp. in season 2012. While the highest infestation was occurred in case of using the insecticides, Aphox (423, 559 and 55.9% resp.& 384, 839 and 72.2%. and Prohpenofos (436, 562 and 57.7% &391, 534 and 51.7% resp.).during 2011 and 2012 respectively. The other treatments occupied the intermediate levels. On the other hand, the yield of tomato fruits was increased by 75% over the check in case of plants treated with Trichoderma viride isolate, but the pesticide treatment given weak or no impact in increasing tomato crop. In season 2012, the infested tomato fruits percentages with T. absoluta larvae and Rhizoctonia soil rot disease(that Frequently observed incidentally) were recorded the minimum levels obtained by spraying Trichoderma viride isolate(21.38 and 10.32% resp.). In contrast, the maximum levels were recorded in Prophenofos (44.87 and22.78% resp.) and Aphox treatments(47.22 and 27.78% resp.).
65-69.Vol 12No 32006
The resistance of peach cultivars to the most important diseases caused by fungi, bacteria, viruses and mycoplasms was studied in Romania over two working stages in the period between 1985-2005. The major diseases examined were: Cytospora cincta Sacc., Taphrina deformans (Berk) Tul., Moruluua laxa (Aderh. & Ruhl) Honey, Sphaerotheca pannosa va...r. persicae Woron., Stigmina carpophila (Lev) M.B.Ellis, Pseudomonas syringae pv. syringae, mycoplasm and plum pox potyvirus. Based on the obtained results, the studied cultivars were classified into resistance groups for the different diseases (very resistant, resistant, medium resistant, sensitive and very sensitive).Based on the results of our study, the following gene sources were chosen following the evaluation of the various genetic material in the peach germoplasm fund, in the climatic conditions of Romania: Cytospora cincta: Cullinan, Cardinal, Hamlet, NJF 3, Onakita Gold, Triumf, "Superba de Toamna", Anderson, Weinberger; Stigmina carpophila: Armgold, ARK 109, Stark Early Blaze, Cardinal, Congres; Taphrina deformans: Madeleine Pouyet, Cumberland, Harbelle, Indian Blood, Sulivan, Victoria, Zafrani, Pekin, Naradnji Ranhii; Spaherotheca pannosa var. persicae: Triumf; Congres; Victoria; Armking; Morton; Regina; Nectared 7; ARK 125; ARK 134; Regina.
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