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  • Shoot induction and plant regeneration from cotyledon segments of the muskmelon variety "hógolyó"
    61-64.
    Views:
    139

    Cotyledonary segments of the casaba type muskmelon variety "Hógolyó" were used to induce organogenesis. Fifty different hormone combinations were applied to enhance the induction of shoot formation on the edge of the segments. The phases of organogenesis were followed with light- and scanning electron microscope. Shoot induction was achieved with high frequency. The shoots were transferred to hormone free media for root induction. The rooted plantlets were planted out to soil.

    NAA was feasible and the method can be applied in transformation experiments.

     

  • The effect of soil mixtures of different consistence and phosphorus content in tray transplant-growing by lettuce
    71-76.
    Views:
    115

    The transplant-growing with root balls gets more and more popular and time to time the only method even in high-quality lettuce transplant-growing. To work out the technology of transplant-growing in trays it was needed to define the accurate physical and chemical consistency of applied soils.

    The transplant production in trays could become a good method in field-grown lettuce technologies. The production of transplants of good quality with this technology could be realised only by accurate soil mixtures.

    The matter of transplant-growing substrates could be a low-moor turf. Its qualities could be positively influenced by adding minerals like bentonite in amount of 5 (or 10) V%.

    To ensure enough phosphorus for the demands of transplants we have to add more fertilisers. The best results by lettuce we got by adding 4 kg/m3 superphosphate to the soil mixture.

  • New sweet cherry cultivars in intensive plantings
    13-16.
    Views:
    314

    The study took place in the largest sweet cherry plantation in West Hungary. The purpose has been the identification of those varieties, which will be suitable for intense cultivation, early fruiting and excellent fruit quality, moreover, the selection of the optimal phytotechnical procedures. At the same time, scion-rootstock combinations have been tested also from the point of view of growing intensity and fruiting in high-density plantation. The dense planting is induced to start fruiting early and yield regularly by special methods.Yielding was stimulated by maintaining the balance of vegetative-generative growth by binding the shoots, by summer pruning, by cuts on the trunk and root pruning. Best experiences have been found in yield and quality in the following varieties: Canada Giant, Carmen, Firm Red, Giant Red, Katalin, Kordia, Regina. Dense planting has been feasible also on vigorous rootstock, like P. mahaleb. Dwarfing rootstocks like P-HL-A, Gisela 6, accelerate the formation of flower buds and yielding earlier with fruits of adequate size. ‘Firm Red’ and ‘Giant Red’ excelled with their large fruit (>27 mm diameter) in all combinations, thus being promising under Hungarian conditions.

  • In vitro regeneration from cotyledons of watermelon
    96-98.
    Views:
    163

    Cotyledonary segments of five different genotypes of watermelon were used to induce organogenesis. Five different hormone combinations were applied to enhance the induction of shoot formation on the surface of the segments. The phases of organogenesis were followed with light and scanning electron microscope. Shoots were obtained after four weeks, then the shoots were transferred to hormone free medium for root induction.

    This method of regeneration can be applied in transformation experiments. GUS histochemical assay was made to check the expected success of using Agrobacterium for the transformation.

  • Grape rootstock - scion interactions on shoot growth and cane maturing
    30-34.
    Views:
    172

    Interaction between the rootstock and scion varieties (clones) was examined. Berlandieri x Riparia T. K. 5BB, Berlandieri x Riparia T.5C being the most widely used varieties in Hungary, Fercal, Ruggeri 140 being recently used on special soil conditions ; Georgikon 28 a new established variety and Berlandieri x Riparia T. 8B GK 10 clone were the rootstocks used. The V. vinifera L. varieties were a clone of Italian Riesling and two new hybrids from the same cross population f ( Noble Italian Riesling X Ezerjó) X ( Noble Italian Riesling X Pinot Gris ) }. This study presents the results how the growth habit depends on different scion/stock combinations in the first three years.

    Shoot growth characters were observed during the initial years after plantation in the vineyard until we got the first yields. Our results did not agree with those of Pospisilova (1977) that the anticipated vigour of shoot growth will appear later in the vineyard only. However we agree with Zimmerman (1970), who showed that the differences are caused by the rootstocks in the growth habit of vines during the first two years already. The differences in shoot elongation decreased similarly to the rootstocks in the first year of fruit production. It shows us that the intense root development caused vigorous shoot growth, which we think to be a rootstock effect. When the vegetative and generative development are balanced already, the effect of scions in shoot elongation became stronger than the effect of rootstock.

    We also found great differences in the maturation of wood each year from planting of the vineyard to the year of fruit production.

    During the first years the differences of cane maturation were greater according to the scion varieties, the means were significantly different. In the first ripening year the differences dwindled or increased due to the rootstocks. This means that the effect of the rootstock on cane maturation became stronger in the first year of fruit production.

     

  • The effect of different biostimulators on morphological and biochemical parameters of micropropagated Hosta ’Gold Drop’
    22-29.
    Views:
    236

    During in vitro multiplication of Hosta ‘Gold Drop’, 20 g l-1 sucrose, 5.5 g l-1 agar and 4 concentrations (0.1-0.8 ml l-1) of Ferbanat L, Kelpak, Pentakeep-V were added to half-strength Murashige and Skoog (MS) basal medium. As compared to the control and other biostimulators, plants with lower peroxidase activity, larger fresh weight, more, longer shoots and roots, larger leaves were developed on medium containing Kelpak. The best concentration was 0.4 ml l-1 for in vitro rooting, shoot formation, plant weight and ex vitro chlorophyll, carotenoid level, peroxidase activity. Pentakeep was the less efficient biostimulator, increasing of its concentration mostly decreased root and shoot values (furthermore, abnormal callus formation was observed, as non-wanted effect), chlorophyll content and sizes (length, width) of leaves, not only during in vitro propagation but also (as after-effect) acclimatization because of the high mortality and weakly developed survivor plants.

  • Effect of different non-chemical treatment methods on organic seed
    63-65.
    Views:
    114

    The importance of ecological farming in Hungary is increasing. The utilisation of organic seed is regulated by national law. In our experiment the effect of two plant conditioning substantial: Biokál and Biomit Plussz were tested on the germination of seed. Our test plant species was garden pea (Pisum sativum L.), the most important vegetable in Hungary. The experiment was carried out in the germination laboratories of the National Institute for Agricultural Quality Control after international rules. The germination, the length of root and shoot were measured. 4 hours soaking of pea seeds in 30% solution of Biokál gave the best result. Our results could help to make the field emergence of organic seed faster and safer.