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Acceptance of artificial nesting sites by pollinating mason bees in commercial fruit plantations (Hymenoptera, Megachilidae: mixed Osmia cornuta and O. rufa population)
43-46.Views:822During a four yeas long experiment a simple bee shelterswas found to be a propermethod to increase the size ofmixed natural populations of the early season Osmia cornuta and O. rufa under practical farm conditions. Instead of the number of pesticide applications the earliness of the flowering of the fruit species in the orchards was found to be themost important factor in the rate of acceptance of reed as nestingmediumby Osmias in the bee shelter. This relationship was negative and highly significant (r = –0.829, p<0.001). This means that much higher acceptance of artificial nestingmedia in bee shelters and consequentlymuch higher population increase of the two early seasonmason bee species can be expected in orchards with early than with late flowering fruit species. Mixed orchards or early flowering orchards surrounded by other orchards falling in bloom later consecutively may also be much favourable to mason bees because they can find continuous food (pollen) supply there for a much longer time during their period of activity than in orchards planted with a single fruit species and being apart from orchards of other fruit species.
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Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods
37-42.Views:283Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.
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DNA-based determination of suitable pollinating cultivars for the pear cultivar 'Carola' (Pyrus communis)
15-19.Views:211Pollen-limited fruit set has long been suspected in some relatively low-yielding orchards with the Swedish pear cultivar 'Carola'. Fruit was therefore harvested on 23 'Carola' trees in a commercial pear orchard. The seeds were germinated and five seedlings from each tree were sampled to determine which of the surrounding cultivars had been the most successful pollinators. Leaves of 'Carola', the 7 putative pollinating cultivars and the 115 seedlings were analysed with 6 RAPD primers. By comparison of the band patterns, paternity could be ascertained for 74 seedlings. The by far most successful pollinator was 'Clara Frijs' which had sired approx. half of the seedlings, followed by 'Herzogin Elsa', `Skanskt Sockerpiiron', 'Alexandre Lucas', 'Coloree de Juillet' and 'Doyenne du Cornice'. The latter is the maternal parent of 'Carola', and these two cultivars must therefore share one S-allele and hence can only be semi-compatible. In addition, 6% of the seedlings were in all likelihood derived from selling_ since they showed no bands that did not occur also in 'Carola'. Maximum distance between 'Carola' trees and suitable pollinators should not exceed 15-20 tn. Longer distances may produce a serious dearth of compatible pollen as evidenced by the large percentage of seedlings derived either from selling. (25%) or from long-distance (> 40 m) pollen transfer (25%) when 'Carola' trees were surrounded by non-preferred pollinators.
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Comparison of flower bud development in almond, apricot and peach genotypes
93-98.Views:326The phenological processes of flower bud development of stone fruits during dormancy are not thoroughly known. The yield of these species, especially of almond, apricot and peach is determined basically by dormancy of flower buds, the survival rate of buds during winter frosts and by their ability to develop normal floral organs in the next spring. After the initiation of floral primordia, flower bud development is taking place in continuous space until blooming, though at different speed characteristic to the species. To study flower bud development during dormancy we applied two alternative methods in different genotypes of almond, apricot and peach: (1) examination of pollen development (microsporogenesis), and (2) the measurement of pistil length. The samples were collected from the central part of Hungary during the dormancy period of 2004/2005. The three fruit species differed significantly in the speed of flower bud development, it was the quickest in almond, followed by apricot and peach. In addition to the species, there were significant differences in the process of microsporogenesis and pistil development between genotypes within species and also between the different types of shoots on which the buds were located. On short shoots buds developed at a higher speed, than on long shoots. Based on our observations, on the short shoots the period of endodormancy was shorter with 5-30 days, according to genotypes, compared to the long shoots. This difference, however, decreased to 2-3 days by the time of blooming.
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Comparison of varying pollen source on productivity of sour cherry (Prunus cerasus L.) cultivars
23-26.Views:767Fruit set of five sour cherry cultivars (‘Újfehértói fürtös’, ‘Éva’, ‘Petri’, ‘Pándy 279‘ and ‘Csengôdi’) of eight years-old trees grown in Újfehértó, located in the Eastern north part of Hungary have been studied over two years (2008 & 2009). Following reciprocally cross-pollination, free-pollination, self-pollination (autogamy) artificial self-pollination (geitonogamy) were studied. The results show that both maternal and paternal parent cultivates had significant effects on the percentage fruit set. Significant differences have been found in fruit set among years and among pollination treatments. Fruit set of free-pollinated Pándy 276 cultivar was low and seasonally highly variable. The yield of this cultivar on self-pollinated flowers was nearly 0%, and in this treatment the maximum yields did not reach 10% in any of the examined cultivars. There was no significant relationship in the fruit set of free-pollination and natural self-pollination treatments.
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Variability of the data indicating the fertility of different plum varieties
51-55.Views:686Self-fertility and fertility at conditions of open pollination in plum varieties is strictly determined by genetic factors. However, rates of fruit set are highly variable according to growing sites as well as to seasons, which may result from a couple of inner and outer conditions, but mainly from the method applied in seizing the facts of fertility in the experiments planned including the number of replications of treatments. During three successive years, 4-16 trees of each of the four plum varieties have been selected and 16-64 branches were tagged either for checking their fertility as pollinated freely or isolated excluding the access of foreign pollen. The data of fruit set have been processed in order to determine the variability of the data, subsequently, the number of replications necessary to make reliable decisions. Both autogamy and open pollination displayed multiple differences between branches and trees studied.
A number of 20 branches are needed yearly for each variety, the branches should be distributed on 5 trees at least for checking the autogamy, whereas on 10 trees for the results of open pollination. Each variety and treatment should be represented in three seasons, at least because of the different weather conditions.
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Continuous and discontinuous nectar secretion in some pear cultivars
77-79.Views:192Hourly nectar secretion was studied in five pear cultivars between 1997-1999. Some cultivars (e.g. ‘Csákvári téli') secreted nectar continuously during the whole day, offering both nectar and pollen for pollinating insects. Discontinuous secretion (e.g. cvs. ‘Viki', `Nyárig tartó 6/19') is less advantageous from the viewpoint of insect attraction. In some cases, however, discontinuity or continuity of nectar secretion varied even within a cultivar (e.g. 'Solymári cukor', ’Jó szürke’) in different years.
