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The evaluation of grape vine decline pathogens in the experimental field of the Georgikon Faculty of Agriculture in Cserszegtomaj
19-22.Views:321Vine decline causes important economic loss in viticulture, especially in longer view. Several causal pathogen were described lately, however little is known about the etiology or epidemiology of these pathogens on grapevine rootstock. It is well known that these diseases affect grafted and rooted grapevines and are not specific to any scion-rootstock combinations. Our aim was to determine what pathogens are presents in the experimental field, especially causal agents of the rootstock decline. Different grapevine rootstocks and scion varieties were tested in our trial. Isolations were made from the wood tissue and pathogenity tests were done with isolated Cylindrocarpon destructans. The possibility of infection during the propagation process was studied as well. Most commonly Cylindrocarpon sp. and Phomopsis sp. species were identified from the examined varieties. Cylindrocarpon destructans was able to spread to apical (shoot) and basal (root) direction from the point of infection with uneven speed. Callus development is not inhibited by the fungi causing the leaf symptom of the vine decline. Shoot development is reduced if unhealthy parts are grafted to each other.
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Changes of the characteristics of Satureja hortensis L. herb during flowering period
56-60.Views:264In this study the changes of the characteristics of Satureja hortensis herb were investigated during flowering period, in case of savories of 4 different origins. The change of drying ratio, leaf/stem ratio, essential oil content, the ratio of several essential oil components (carvacrol, y-terpinene, p-cymol, a-pinene, p-pinene and fi-caryophyllene) were studied from bud formation till seed ripening.
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Optimization of fertilizer use efficiency, soil quality and oil palm (Elaeis guineensis Jacq.) growth with biochar under drip irrigation conditions irrigation conditions
31-36.Views:478Biochar (BC) is an eco-friendly product characterized with high content of carbon and usually obtained by heating biomass without oxygen. Utilizing BC as organic material to amend the problematic soils and improve plant growth and yield has been proved previously. This study investigated the effect of vetiver grass biochar (VGB) on fertilizer use efficiency, soil quality, and oil palm growth performance. A net house experiment was conducted at the Farm Unit, UiTM Sarawak Branch, between August 2022 and March 2023. A factorial randomized complete block design (RCBD) with five treatments and four replications was devised. Treatments applied were: T0) absolute control; T1) 100% NPK fertilizer; T2) 100% vetiver grass biochar; T3) 50% vetiver grass biochar + 50% NPK fertilizer; and T4) 25% vetiver grass biochar + 75% NPK fertilizer. The BC application significantly improved the fertilizer use efficiency through reducing the rate of fertilizer applied. It also significantly enhanced most soil-measured chemical properties and soil nutrients. The growth performance of oil palm plant was significantly enhanced by BC application in terms of plant height, hump diameter, leaf number, chlorophyll content, and plant biomass. The BC application demonstrated its usefulness in managing soil and cultivating oil palm plant sustainably by reducing the rate of fertilizer applied and improve the fertilizer use efficiency. Based on the output, we suggest that treatment T3 (50% vetiver grass biochar + 50% NPK) can be used to improve the growth performance of oil palm.
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Integration of terrestrial laser scanning and spectral canopy scanner in horticulture applications
15-18.Views:1043One of the most difficult challenge in the everyday practice to describe the canopy growing of fruit trees in an orchard. The photosynthetic activity is the basic of the primer production of plants. The measurement of leaf area and determination of the photosynthetic activity could be occurred with some elaborated methods between experimental conditions. In this article we present such an integrated methodology, which is ideal to determine the geometric and spectral characteristic of fruit trees between field conditions.We have carried out laser scanning technology to investigate the geometric-topological characteristics and parallel the active infra-red sensor to collect spectral data about an apple orchard. The surveys were worked out in an intensive apple orchard with drip irrigation system, protected by hail net in Study and Regional Research Farm of the University of Debrecen near Pallag. This study shows the filtering and interpretation methods of created data. The produced high accuracy data can be directly used in the precision horticulture. It could serve as a guiding data to implementation a future “virtual horticulture”. Higher spatial and temporal resolution could help for a better recognition of water balance of orchards.
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Comparative investigation on Hypericum perforatum L. populations of different origin
56-60.Views:448Widespread application for herbal medicines based on Hyperici herba has been experienced in the last few years, especially in the treatment of depression syndrome. As the wild origins could not satisfy the market demands neither in quantitative nor in qualitative respect, the necessity of the development of intensive growing methods has been raised. In the course of our investigations we intended to clear up the morphological and chemical variability among and within populations of different origin in order to start a new breeding program. According to our data, growth dynamics of populations could be characterised by a logistic curve. According to the time of flowering the populations formed early, middle and late groups. Morphological diversity among populations was measurable in differences of flower length, plant height, different leaf types and plant habit, according to which characteristics groups were distinguished. Generally, the accessions were the most homogenous in plant height (CV: 7-15%), followed by inflorescence-lengths (CV: 11-36%) and the least uniform characteristics proved to be the number of flowering shoots (CV: 14-59%). The greatest morphological heterogeneity was experienced in the accessions of wild origin as it has been expected. In the second vegetation period generally a much better homogeneity was obtained, than in the first year. The most outstanding accessions produced 1.2 t of dry flowers and 4 t of dry flowering shoots, calculating to one hectare area. The individual yields varied on a large scale in each population (CV: 18-70%).
The content of hypericin varied between 1,85 and 9,9 mg/g in 1996, and between 0,18 and 2,70 mg/g in 1997, showing high individual divergences. Flavonoid values -including first of all hyperosid, rutin and quercitrin - reached 17-39 mg/g in the first and 15-20 mg/g in the second year, respectively. Individual and seasonal variation was less than in hypericin. A joint high level of these two compound groups seems to have low frequencies.
The superior population were selected for further breeding.
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Estimating of water consumption of cherry trees
15-17.Views:398Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday- I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.
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Effects of aromatic cytokinins on structural characteristics of leaves and their post- effects on subsequent shoot regeneration from in vitro apple leaves of 'Royal Gala'
41-46.Views:407The effects of different aromatic cytokinins applied in different concentrations and combinations were investigated on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves were studied. Great differences in the anatomical structure of leaves could be detected originating from media containing different types and concentrations of aromatic cytokinins. The number of regenerated shoots per explant and the organogenetic index were used for the evaluation of the post-effect of aromatic cytokinins on shoot regeneration. The histological structure of leaves used for regeneration and their regeneration response showed a good correlation. When the pre-treatment caused a juvenile-like or less-differentiated structure, the number of regenerated shoots per explant increased and often vitrification also decreased and consequently the organogenetic index also increased. A strong interaction between cytokinin-content (type and concentration) of the pre-treatment medium and that of the regeneration medium could also be detected.
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Anatomical study of the bud union in „Chip" and „T" budded 'Jonagold' apple trees on MM 106 rootstock
27-29.Views:693The traditional methods for vegetative propagation of apple and its varieties are the T-budding, and the winter grafting, but this latter way is a difficult and expensive procedure.
In our experiment carried out in the Fruit Tree Nursery Soroksár, the healing process of chip- and T-budded apple trees 'Jonagold' on MM 106 rootstock was studied.
The budding (T- and Chip-) was made in the first week of August, samples for microscope examination were taken monthly after this time until leaf fall.
The investigated part of plants was made soft with 48 % HF (hydrogenfluoride), then cross and longitudinal section were made and examined by microscope.
Based on analysis of microscope pictures in case of Chip-budding, it was established, that development had started quickly after budding on the rootstock and scion too. But the callus originated almost entirely from the rootstock tissue as new parenchyma cells fills the gap between the two components of graft (scion and stock), becoming interlocked and allowing for some passage of water and nutrients between the stock and the scion. This quantity of callus in case of T budding was under the scion buds larger, than the Chip-budded unions, where the thickness of callus mass is uniformly thick round the chip. The large mass of callus pushes the scion bud outwards from the shoot axis, which later results in a larger shoot-curvature above the bud union.
Following this process on the Chip-budding it can be observed also, that a continuity of the cambium is established between bud and rootstock. Then the newly formed cambium started typical cambial activity, forming new xylem and phloem.
Later the callus begins to lignify, and it is completed within about 3 months after budding.
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Preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard
35-37.Views:465In this study, the objective was to report a preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 11 and 20 on shoot and between 9 and 24 on fruit. Number of asymptomatic plant part ranged between 85 and 109 on shoot and between 133 and 206 on fruit. Disease incidence ranged between 13.8 and 17.6% on shoot and between 9.1 and 11.3% on fruit. Disease aggregation index ranged between 0.098 and 0.228 on shoot and between 0.043 and 0.108 on fruit. One of the four trees showed significant within canopy aggregation of disease for shoot powdery mildew symptoms in both years. For leaf powdery mildew, all tree exhibited random patterns in both years.
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In vitro multiplication and hardening of grapevine plants in aeriated media
15-18.Views:378In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.
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Results of cherry rootstock evaluations in Hungary
11-14.Views:434The paper gives a review on the results of the latest rootstock evaluation projects in Hungary. Several cherry rootstock evaluation projects were carried out in Hungary during the last two decades. The evaluated rootstocks are partly mahalebs selected in Hungary, but more or less all the most important new rootstocks from different countries are involved. The aim of these evaluation projects was to find appropriate rootstocks in a wide range of vigour for our climate and soil conditions. The conclusion of the last 20 years of research in Hungary proved just the opposite of believes, that high density orchard can only be planted with dwarfing rootstocks. Before choosing the right rootstock the most important is to consider adaptability, precocity and productivity. Dwarfing and semi-dwarfing rootstocks showed proper results only with irrigation or very good site conditions. These rootstocks are very precocious, but branches form easily blindwood. This must be corrected by severe pruning in the first years. The relatively small leaf surface area can also be disadvantageous, but it can be controlled by pruning, fruit-thinning, irrigation and fertilization. After studying different rootstocks semi-vigorous rootstocks seem to be the most adequate for different site conditions in Hungary. Fast initial growing and competent precocity are their favourable characters. From the vigorous group of clonal mahalebs or seedlings are highly recommended first of all for poor site condition (sandy, lime soils with high pH). They turn to bearing early and easy to find them in the Hungarian nurseries. But on vigorous rootstocks trees might need stronger summer pruning.
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Bud-, flower- and fruit-density in stone fruits
59-69.Views:985In 164 varieties of five stone fruit species, counts of flower buds, flowers and fruits set have been performed, regularly, between 1982 and 2002. The critical number and sample size has been determined for the purpose to estimate the yielding potential of peach plantations. For a rapid test, 10 shoots per variety are recommended. In sour cherry and peach varieties, the number and ratio of leaf and flower buds has been assessed on bearing shoots of different length.
The typical flower bud density of 129 peach varieties varies, as a rule, between 0.13 and 1.10 bud/cm. Three groups of flower-bud-densities could be distinguished: low (0-0.40 bud/cm), intermediate (0.41-0.60 bud/cm), high (more than 0.60 bud/cm). About 62% of varieties belong to the intermediate group. Negative correlation has been found between flower density and relative fruit set, whereas positive correlation between flower density and fruit yield.
The results are utilised in the description and choice of varieties, moreover, in choosing of optimal pruning policies. Varieties of high flower bud densities are recommended to be preferred for growing sites with frequent late frosts. Abundantly yielding varieties of low vegetative vigour are to be pruned more severely than those characterised by low yields, vigorous growth and low flower density. Sour cherry varieties, which are inclined to grow "whips" ought to be stimulated to grow longer shoots (40-50 cm per year), than varieties woid of that tendency (30-40 cm).