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Increment analysis in black locust (Robinia pseudoacacia L.) stand – a case study
106-109Views:505The study on the diameter and volume increment of black locust (Robinia pseudoacacia L.) stand (age 10-27 years, yield class II) have shown that in comparison to the periodic annual increment of the breast height diameter of tree in height class I, trees in height class II have reached 83.3%, while in class III only 43.9. The same relations for volume were found 59.0% and 24.5%, respectively. The mean values of the whole stand were close to those of height class II. According to the distribution of the periodic annual increment of volume between 10 and 27 years of age, 50% of the values were between 2.00 and13.88 dm3, 73% were between 2.00 and19.82 dm3, and 96% were between 2.00 and 37.64 dm3. The range of 13.88–19.82 dm3 had the highest occurrence (24.5%).
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Real-case application of mycorrhizal inoculums on Capsicum annuum L. var. longum cv. Szegedi and Kalocsai
75-79.Views:317The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, a mixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF) was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights of shoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weights due to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative crop production compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai. Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsai and Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonization was observed in June in general and colonization percent increased during vegetative development and there was a slight decrease at harvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spice pepper production.
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Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA
101-104.Views:250Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-life has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.
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Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia
74-83.Views:356Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.
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Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:288A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
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Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
75-79.Views:254Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.
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Evaluation of hot pepper (Capsicum annum L.) varieties for green pod yield and quality under rain fed production at Teppi, South Western Ethiopia
45-49.Views:383The trials were designed with three replications in a randomized complete block design in order to evaluate the phenological, growth and yield potential of hot pepper varieties such as ‘Mareko fana’, ‘Melka Zala’, ‘Melka Awaze’, ‘Melka Shote’ and local check. Significant difference was observed between growing year and used varieties. The varieties also performed significantly different (p<0.05) for most of the considered traits in the study. The result revealed that varieties ‘Melka Zala’, ‘Mareko fana’ and ‘Melka Awaze’ were scored highest green pod yield of 8.39, 8.71 and 11.39 ton per hectare, respectively. However, ‘Mareko fana’ variety was susceptible to disease attack as compared to other varieties. Therefore, promoting both ‘Melka Awaze’ and ‘Melka Zala’ varieties for widespread production for Teppi and the areas with similar agro-ecological conditions could contribute to boost the productivity of hot pepper. ‘Mareko fana’ could also be used for dry pod purpose due to its attractive color.
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The inheritance and durability of scab resistance in apple progenies
39-46.Views:365In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
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Investigation of the in vitro regeneration of mericlones in the caribe variety of carnation
87-89.Views:235In vitro culture conditions were experimented for the relatively sensitive, but very esthaetic "Caribe" variety of carnation with uniformly dark violet flowers. Regeneration of new plants from shoot apex meristems can be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. Callus formation as a prerequisite for the induction of somaclonal variability can be achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Acclimation of the obtained mericlones to the ex vitro conditions was also evaluated.
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Effects of irrigation frequency and repellent plants on false codling moth (Thaumatotibia leucotreta) infestation, growth, yield, and quality of sweet pepper (Capsicum annuum L.)
34-40.Views:96Sweet pepper (Capsicum annum L.) is an important vegetable and spice crop grown worldwide for its pungency, which is derived from high concentrations of capsaicinoids. Despite the economic importance of sweet pepper as an export vegetable in Kenya, its production is currently constrained by the false codling moth (FCM) (Thaumatotibia leucotreta) and inadequate management strategies for the pest, among other factors. This study, therefore, evaluated repellent plants (Artemisia (Artemisia absinthium), Lavender (Lavandula angustifolia), and Spearmint (Mentha spicata) under different irrigation frequencies to control FCM. A split-plot factorial greenhouse experiment was conducted at Kenya Agricultural and Livestock Research Organization, Muguga (trial 1) and the Horticulture Research and Teaching Field, Egerton University, Kenya (trial 2). Irrigation frequency at three levels (irrigating once a week, irrigating twice a week and irrigating thrice a week) was the main-plot factor, while repellent plants at four levels: sweet pepper (Capsicum annum L.), artemisia (Artemisia absinthium), lavender (Lavandula angustifolia) and spearmint (Mentha spicata) constituted the sub-plot factor. Irrigating thrice or twice a week and using lavender as a repellent plant significantly reduced false codling moth infestation by 90%, increased yield by 28% and improved fruit quality in terms of fruit collar diameter and total soluble salts (TSS). The findings demonstrate that integrating lavender repellent plants with frequent irrigation can be adopted as an eco-friendly management strategy for FCM and enhancing sweet pepper yield and quality.
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Vegetative parameters of sweet cherry (Cerasus avium L.) cultivars in two training systems
42-45.Views:774Hungarian sweet cherry production has been changed recently. Thanks to the favourable selling prices many new plantations were established in the last years. In the new orchards mainly spindle canopy are trained, although many type of crown forms are used all over the world. In our study slender spindle and bush canopy were evaluated with three cultivars (‘Petrus’, ‘Vera’, ‘Carmen’) grafted on Prunus mahaleb rootstock. Vigor of the 3-4 years old trees showed spectacular differences. The highest vigor with Spanish bush was found for cv. ‘Vera’, but on slender spindle canopy the thickness values were medium. Meanwhile cv. ‘Petrus’ showed the highest trunk thickness with spindle canopy, but the lowest with Spanish bush. On spindle canopy cv. ‘Carmen’ presented the highest ramification ability, but its 2-3 years old twigs started to bald very early, which can be considered an unfavorable phenomenon. Bush canopy still can be described with strong vegetative growth on 3-4 years old trees, as vigor of spindle trees are more moderate.
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Pruning of plum trees and maintenance of balanced yielding
131-138.Views:560In this review, we give an overview of the pruning techniques that should be applied in commercial plum orchards. First, a detailed description of pruning for shaping the different crown types for the first, second, third, fourth and fifth years and the phytotechnical works is given for crowns with a circular projection area. Then, pruning for open crowns forms, such as the vase form is discussed. Following the shaping of the crown, it should be maintained by pruning. Detailed information is given on pruning for maintenance with special emphasis on the comparison of winter and summer pruning. In the following part of the manuscript, pruning experience is described separately for the major commercial plum cultivars in Hungary such as Cacanska lepotica, President, Bluefre, Stanley. Recommendations for cultivation systems under non-irrigated conditions are also included. Finally, light requirements, growth characteristics and suggested crown forms of the different cultivars are described for cvs. Ageni, Althan Tingle), Besztercei szilva Nm. 122, Bluefre, Cacanska lepotica, Cacanska rodna, Debreceni muskotály, Montfort, President, Ruth Gerstetter, Sermina, Stanley, Tuleu gras and Zöld ringló).
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Factors affecting rhizogenesis in vitro and acclimatisation of three cherry rootstocks
40-46.Views:237Factors affecting rhizogenesis in vitro and acclimatisation of three rootstocks of cherry, i.e. Mahaleb, Maxma-14 and Weiroot -10 were investigated.
Rooting was easily achieved within 2-4 weeks on MS-based liquid or agar-gelled media containing auxins IBA at conc. 0.49 or 2.45 pM or NAA at conc. of 0.49 pM. On liquid media with 2.45 pM IBA, a maximum rooting efficiency of 95-100% was obtained. However, high concentrations of auxin delayed the time of root initiation for 3-5 days.
Rooted plantlets were transplanted into pots with a mixture of 3:1 (v/v) peat:perlite and acclimatised gradually to field conditions with efficiency of 60%.
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Review on rain induced fruit cracking of sweet cherries (Prunus avium L.), its causes and the possibilities of prevention
27-35.Views:1163The rain induced fruit cracking is a big, serious and costly problem for cherry growers. Cracked fruits lose their value and they are not marketable because of the poor fruit quality. Cracked fruits have different storage diseases and shorter storage and shelf life.
There are many influencing factors of the cherry fruit cracking such as: water uptake; fruit characteristics (fruit size, fruit firmness; anatomy and strength of the fruit skin, stomata in fruit skin, cuticular properties, osmotic concentration, water capacity of the fruit pulp, growth stage of the fruit,); orchard temperature and other environmental conditions;
The most effective protection technique is the plastic rain cover over the tree rows. The installation of these equipments is too expensive for the cherry growers. That is the reason why researchers tried to find other less expensive and sufficiently effective ways against the Lim induced fruit cracking.
Several calcium formulas: calcium chloride (CaC1,), calcium hydroxide (Ca (OH)2) and calcium nitrate (Ca(NO3)2 ) can be sprayed in appropriate concentration. Spraying with other mineral salts (aluminium and copper salts, borax) and PBRs (Plant Bioregulators) may be also effective to reduce fruit cracking.
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Nutrient demand of stone fruits
19-23.Views:259Effects of nitrogen, phosphorus and potassium fertilization were investigated on the change of nutrient content, vegetative and generative production of apricot, peach and sour cherry trees, as well as on frost hardiness in long term experiments. Nitrogen and phosphorus fertilization increased only the concentration of these elements in cherry leaves without effect on growth and yield. Consequent potassium effect was proved on these stone fruit species. Effect on yield appeared following the first higher crop load.
Potassium supply has positive effect on frost hardiness of apricot and sour cherry flowers and peach flower buds.
In peach, the lime content of soil decreased the yield but it could be compensated by potassium dressing to some extent. Favourable nutrient boundary values were determined for soil and foliage.
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Role of hydrogen peroxide and Pharmaplant-turbo against cucumber powdery mildew fungus under organic and inorganic production
39-44.Views:875Cucumber leaves have been sprayed with a solution of hydrogen peroxide (H202) or Pharmaplant-turbo combined with organic or inorganic fertilizers under plasic house. Under the influence of H202, leaves exhibited resistant against Podoshaera fusca fungus, the causal agent of cucumber powdery mildew. H202 (15 mM) was able to decrease the disease severity from 90.4% to 12% in two experiments conducted in two seasons. Pharmaplant-turbo (Turbo) is new chemical compound and used as an antifungal compound. Turbo in 1 ml/L was able to decrease the disease severity from 90.4% to 11.5% in the both experiments as well. Both of H202 and Turbo were combined with organic treatment (compost + compost tea + seaweed extracts) which showed significant effect against cucumber powdery mildew fungus and strongly suppressed it as compared to control leaves. Organic treatment produced higher vegetative growth characters and greater early and total yields as compared to inorganic treatment, also organic fruits produced the lower nitrate content and the higher ascorbic acid content as compared to inorganic fruits. Our study have indicated that, H202 and Turbo combined with organic fertilizers play a role in the resistance of cucumber against powdery mildew by decreasing the disease severity. We suggest to give more attention to the direct application of H202 in low concentration and Turbo against powdery mildew diseases and other plant diseases.
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Bee pollination and association of apricot varieties
20-24.Views:1038Apricot yields are highly variable according to the season. The variation is caused mainly by the adversities during the critical processes of floral biology, i.e. blooming and fertilisation. On the basis of information concerning blooming time and mutual compatibility relations of apricot varieties a system of securing regular and adequate yields has been developed.
Winter frosts of the continental type are well tolerated by most of the apricots, however, after the end of rest period, flower buds are loosing frost tolerance, 'rapidly.
Being one of the fruit species blooming earliest during the early spring, apricot start to bloom in Hungary around the end of March or early April as a mean of many years, but it also happened, exceptionally that apricot started to bloom at February 20 (at Letenye South Hungary). The early season, exposes the floral organs to frost injuries. As a consequence, apricot orchards on the Great Plain produce low yields in 3 years, intermediate yields in other 3 years out of a ten-year-period.
Moreover, weather conditions during the blooming period are often unfavourable for pollination. Cool, windy and rainy weather prevents the flight of insects and on the other hand, warm spells shorten the blooming process, nectarines and stigmata get dry and the female gametes loose viability before effective pollination occurres.
The fertility of individual cultivars are meeting different obstacles. Apricot cultivars differ greatly in the rate of flowers bearing underdeveloped pistils, which may attain even 60% (e.g. Orangered). New commercial cultivars are often self-incompatible. Local varieties of that type in Hungary are the „óriás" varieties (e.g. Ceglédi óriás, Szegedi mammut), and the new hybrid Ceglédi Piroska. Many of the cultivars are variable in their self-fertility (partially self-fertile): Budapest, Harmat, Korai piros, Mandulakajszi.
Inter-incompatibility is also known in apricots. The „óriás " varieties do not fertilise each other. During the growth of fruits, cool spells (2-4 °C) caused severe fruit shed in Ceglédi óriás.
Apricot flowers produce pollen and nectar at average rates related to other fruit species, thus bees are attracted sufficiently. Bee visits are very variable according to growing site and season. Most of the bees are pollen gatherers but sometimes nectar suckers are in majority. Bee pollination is necessary not only for the self-incompatible varieties but also to enhance the yield of self-fertile varieties.
Taking the blooming and fertility relations of the cultivars into account, plantations should not exceed two rows to a particular self-incompatible varieties, and possibly two different polliniser varieties are suggested to be planted as flanking the block in question.
In commercial plantations 2 to 4 bee colonies per hectare are proposed to move for the whole blooming period.
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Interaction impact of drought stress, nutrient-deficient water, and seed-borne pathogen (Alternaria alternata) on germination and vigor of two tomato varietiesination and vigor of two tomato varieties
48-53.Views:316Tomato (Solanum lycopersicum) is considered one of the leading vegetable plants in the world. This study evaluated the germination and vigor capabilities of ‘Marmande’ and ‘Kecskeméti Jubileum’ varieties under different conditions, including drought stress, nutrient-deficient water, and the effect of seed-borne disease caused by Alternaria alternata when prime and non-prime with salicylic acid. The experiment was conducted in the laboratory in 2023 at Agricultural and Food Sciences and Environmental Management Faculty of University of Debrecen. Results indicated that the ‘Kecskeméti Jubileum’ variety exhibited a superior strength to ‘Marmande’ in the seedling’s dry weight, seedling growth rate, and vigor index under nutrient-deficient water. Nevertheless, when applying levels of drought, the ‘Marmande’ variety had a higher viability rate of 62.5% in comparison with another variety, and the germination rate of the two tomato varieties is at 85.5% in 5% concentration but decreased progressively when exposed to a higher drought concentration of 10%. When using 3 ml of salicylic acid during germination stages, the seedling vigor index of ‘Marmande’ shows a greater index at 165 compared to ‘Kecskeméti Jubileum’, just 108 under the infection of Alternaria alternata. The results of the examination of drought stress, and the effect of Alternaria alternata, one cause of seed-borne pathogens, showed that the percentage germination and vigor ability of the ‘Marmande’ variety performed better than ‘Kecskeméti Jubileum’ under the same conditions.
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Estimating of water consumption of cherry trees
15-17.Views:398Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday- I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.
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New sweet cherry cultivars in intensive plantings
13-16.Views:963The study took place in the largest sweet cherry plantation in West Hungary. The purpose has been the identification of those varieties, which will be suitable for intense cultivation, early fruiting and excellent fruit quality, moreover, the selection of the optimal phytotechnical procedures. At the same time, scion-rootstock combinations have been tested also from the point of view of growing intensity and fruiting in high-density plantation. The dense planting is induced to start fruiting early and yield regularly by special methods.Yielding was stimulated by maintaining the balance of vegetative-generative growth by binding the shoots, by summer pruning, by cuts on the trunk and root pruning. Best experiences have been found in yield and quality in the following varieties: Canada Giant, Carmen, Firm Red, Giant Red, Katalin, Kordia, Regina. Dense planting has been feasible also on vigorous rootstock, like P. mahaleb. Dwarfing rootstocks like P-HL-A, Gisela 6, accelerate the formation of flower buds and yielding earlier with fruits of adequate size. ‘Firm Red’ and ‘Giant Red’ excelled with their large fruit (>27 mm diameter) in all combinations, thus being promising under Hungarian conditions.
