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A critical evaluation of methods used for S-genotyping: from trees to DNA level
19-29.Views:368Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.
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General defense system in the plant kingdom III.
45-54.Views:283Our observations regarding the symptoms not fitting into, significantly differing from the hypersensitive defense system, which we noticed during the judgment of several plant species, symptoms provoked on several million plants have constituted a unified entity. They have provided evidence for the existence of a different plant defense system. We called this so far unknown basic response of plants to biotic effects as general defense system. This system defends them from the attack of numerous microbe species in the environment.
The evolutionary intermediate phase between the general and the specific, the two defense systems is the susceptible host—pathogen relation. The vertical resistance system of plants escaping from the susceptible host—pathogen relation, based on specific hypersensitive reaction also suggested the existence of a more original, general defense system and the susceptible host—pathogen relation developed as a result of the collapse of that system.
The evolutionary relation of the two defense systems is proved by the only recessive inheritance of the older general defense system and in the majority of cases dominant hereditary course of the specific defense system. In our experiences, the modifying genes of the recessive general defense system, in most cases, are behind the specific defense systems, which are known to have monogenic dominant hereditary course and react with hypersensitive tissue destruction. This seemingly striking genetic fact is explained by the following: the general defense system less dependent on environmental effects regulates much faster pathophysiological reaction than the specific resistance genes strongly dependant on environmental effects coding dominant hypersensitive reaction.
The general and specific defense reactions, the processes excluding the microbes attacking plants with compacting of cell growth and tissue destruction, which mean two opposite strategies, building on and regulating each other constitute the entity of resistance to plant disease.
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Assessment of different rates of NPK fertilizer on the growth and yield components of two exotic okra (Abelmoschus esculentus L.) western urban of Sierra Leone
55-59.Views:498The experiment was conducted in an Inland Valley Swamp during the dry spell of 2017 laid out in a randomized complete block design (RCBD) three replications at the Kabala Horticultural Crops Research Centre (KHCRC) cropping site, Ogoo Farm of the Sierra Leone Agricultural Research Institute (SLARI), Freetown, Sierra Leone. Raised beds measuring 5m x 1m (6 m2) with a 1m furrow between beds. Inter and intra row spacing of 75 cm by 50 cm with two rows per bed and two seeds per hills were directly sown. The treatments constituted NPK 15:15:15 at the rate of 0, 90, 120 and 150 kg/ha designated as T1, T2, T3 & T4 and Two hybrids of okra were used as experimental materials obtained from Indo-American Hybrid Seed Company, India; Okra-WASA-HyOKR-1 and Okra-WASA-HyOKR-2. Seeds were treated with Thiram Poison for longevity. Result indicates T2 (25.2; 35.1 cm) and T4 (56.9 cm) treatments recorded tallest plant heights followed by T2 (24.0, 34.4 & 50.1 cm), while T1 (18.8, 28.4 & 33.3 cm) had the shortest plant heights at 4, 8 & 10 Week After Planting respectively. Similarly, T4 & T2 (38.9, 65.5 & 44.8 cm) exhibited the largest SG and T1 (21.7, 33.0 & 52.0 cm) the least values at 4, 8 & 10 Week After Planting. While, the widest and narrowest LA at 4, 8 & 10 Week After Planting was observed in T3 (131.3, 135.0 & 158.5 cm2) and T1 (120.8, 121.8 & 128.1 cm2). With respect to yield and yield components, the longest (22.0 cm) and heaviest (112.7 g) fruit were recorded by T3 followed by T2 (19.1 cm & 101.3 g) with T1 having the shortest (11.7 cm) and lightest (52.3 g). The width of pod in T3 (31.2 cm) was greater than the rest of treatments and as usual, T1 indicated the least value (14.6 cm) respectively. The average NP produced plant-1 was shown by T2 & T3 (4.0) with the least of fruit produced by T1 (2.0). Also, 100 seed weight, T3 (106.7 g) weighted the highest followed by T2 (103.3 g), while T1 (60.3 g) accounted for the lowest seed weight. Conclusively, the two materials are adaptable to the climatic conditions of Sierra Leone (Western area). Hence further evaluation required across vegetable growing zones.
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Temporal changes of the frequency of spring frost damages in the main fruit growing regions in Western Hungary and in East Hungary
81-87.Views:861Most of the risk in Hungarian fruit growing is the damage caused by late spring frosts. The frequency of late frosts seems to increase nowadays. The aim of the study was to check this contention: what is the real probability of the damages. Based on earlier experiences, the physiological LT50 function has been elaborated for new fruit varieties, which are eligible to moderate the danger when being threatened by frost. By means of this technique, the probability of freezing is distinguished between frost susceptible, frost resistant and medium frost resistant fruit species and varieties around their blooming time. The degree of frost damage depends on the duration and severity of the low temperature and not at least on the frost tolerance of the plant. For that purpose, the frequencies of frost damages were studied at two Transdanubian and two Trans-Tisza fruit growing sites by means of a meteorological database for the 60-year-long period 1951–2010. Being aware of the LT50 values changing during the phonological phases of the fruit trees from budding, bloom, fruit set and fruit growth, the number and date of critical (frosty) days could be settled. An important role is attributed to the orographic relief and the height above the sea level of the site, as 20–30 m differences and expositions may become decisive within the same plantation. The spatial distribution of damages is also dependent on the air circulations within the Carpathian basin. At the southern and northern borders of the country, especially valley bottoms represent additional risks of frost. Most spring frost damages are experienced in April 20–22, and cause heavy damages by temperature minima between – 3°C and – 6°C. The severity of damage depends largely on the temperature of the preceding few days. The earlier bloom the heavier damage is expected. The study is emphasising the importance of the varieties. Frost tolerance of some varieties may lower the risk of spring frosts by 40–50%, as experienced on the plantations. The quantifi cation of the risks based on data raised during the last years will be suitable to defi ne the security of yields of each growing site successfully.
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Production technology and fruit tree nutrition
39-42.Views:276Fruit yield quality and quantity are effectively enhanced if healthy vegetative conditions are ensured. These optimal conditions — i.e. the balance between shoot development and yield — can be achieved by the rationalization of the production technologies, such as:
- reduction of the size of the crown
- adaptation of the severity and method of pruning to the conditions of the actual year
- removal of the shoot tips
- timely fruit thinning
By establishing an improved level of plant nutrient uptake, this will ensure a healthy balance between shoot growth and yield.
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Volumetric estimation of an intensive apple orchard with GIS
7-10Views:691Remote sensing instruments and methods have become widespread in all segments of agriculture and horticulture. Among the innovative development of remote sensing instruments, the 3D laser scanner is one of the newest technique, which overtop in the spatial data collection. Terrestrial laser scanning is an appropriate tool for identifying such biophysical parameters like the structure of trees and branches, growth of volume. Determination of these parameters are time consuming and complicated with traditional methods. The examination was carried out in the Study and Regional Research Farm of the University of Debrecen near Pallag. We used Leica ScanStation C10 terrestrial laser scanner to estimate volumetric properties of the intensive apple orchard. Two rows of the study area were surveyed, where the height and stem diameter of apple trees were measured with Geomagic Studio 12 GIS Demo Software. Based on the built-in algorithms, the volume of each tree were defined by the software. The measured and calculated data was correlated, and middle correlations were detected. Estimated volume results could be useful for further investigation such as CO2 fi xing, which is an important factor for ecological service of the plantation.
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The effect of climatic anomalies on the nutrient supply of fruit plantations (Minireview)
111-116.Views:11451Climatic conditions play an important role in agricultural production. It has a profound influence on the growth, development and yields of a crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water stresses. Nowadays, we have to know the dark side of the weather events because it is causing more and more problems and significant hazards to many horticultural regions in Hungary. The aim of this study is to explore the problems of nutrient uptake following climatic anomalies and response. These problems are: (i) water supply problems (water-stress); (ii) drought and frost as temperature strees. Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
- Nutrient demand of trees can be supplied only under even worse conditions.
- The most effective weapon against damage of climatic anomalies is preventative action.
- When developing a fruit orchard, three factors should be taken into consideration: "Location, Location, Location".
- Moreover, proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of climatic anomalies.
- Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
- Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar fertilization is recommended, which adjusted to phonological phases of trees.
Another solving is groundcover of soil means a potential opportunity to temper or even avoid climatic anomalies.
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Giant reed (Arundo donax L.) from ornamental plant to dedicated bioenergy species: review of economic prospects of biomass production and utilization
39-46.Views:767Giant reed (Arundo donax L.) is a perennial, herbaceous grass, it has been spread all over the world from continent to tropical conditions by human activities. In continental climate, especially Hungary, it has been considered as ornamental species, due to its decorative appearance, striped variants’ colour of leaves, long growing season and low maintenance requirements. It does not produced viable seeds, so it can be propagated vegetative ways by rhizomes or stem cuttings and by in vitro biotechnology methods. Because of its growth habits and good adaptation capability, it has been considered invasive weed primarily in coastal regions in warmer climate areas. In the previous century, giant reed produced for paper/cellulose/viscose production, woodwind musical instruments, stakes for plants or fishing rods etc. Over the last few decades, it has been produced for bioenergy purposes (bioethanol, biogas, direct combustion) or utilize as chemical basic compounds or construction materials. It has been considered a dedicated promising biomass crops thanks to high biomass production, high energy balance of cultivation and adaptability of different kind of soils and conditions. The objective of the present paper is to overview the most significance literature data on giant reed production and utilization, compare to own experimental data and economic calculations and to determine some critical factors, advantages and disadvantages of giant reed production compare to other biomass species.
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Summer pruning of sweet cherry trees and an inquiry of winter frost damages
41-44.Views:8810One of the most demanded research projects is the intensification of fruit production. The use of dwarfing stocks is a moderate solution as their effect is scarcely satisfactory. Climatic conditions of Hungary are continental in Eastern Europe, where Atlantic and Mediterranean effects are interacting with the continentals in a kind of basin with characters of its own. Capricious meteorological episodes are often disturbing the security of development and fruiting of trees:
• winter frosts are damaging the cambium and fruiting structures of trees
• late spring frosts destroy cambium and flowers
• early autumn frosts hurt the leaves
• excessive precipitation impairs the growing fruits
• drought periods during the summer caused water stress disturbing water husbandry.Vigorous stocks still prevail in the practice, and they ought to withstand challenges of weather hazards. The strong vigour of plants delaysthe process of senescence and the tendency of getting bald, and regeneration of plants is a sign of vitality. In present research, the trees have been trained on vigorous Prunus mahaleb stocks. Summer pruning was one of the important tools of intensive growing techniques. They were compared with traditional techniques and with plastic foil protected trees observing the vegetative as well as generative growth of them.
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Micropropagation of an orchid Dendrobium strongylanthum Rchb.f.
61-64.Views:442A simple and reliable procedure for in vitro propagation of an orchid Dendrobium strongylanthum Rchb.f. was studied. Protochorm was induced from seed explants on 1/2 MS medium supplemented with 0.2 mg/L NAA. A mass of protochorm could be multiplied on proliferated medium of 1/2 Ms containing 0.5 mg/L V-6-BA. And bud differentiation of green global body was cultured in the same media, 2-2.5 cm shoots were formed after 30 day of culture. Addition of mashed banana and 0.5 mg/L NAA to 1/2 MS medium promoted root formation and vigorous growth. The plantlets were acclimatized and transplanted to compound materials of humus:sawdust (1:1) in greenhouse, the survival rate was more than 98%.
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Pedological and agrochemical investigations on media using in vegetable forcing
119-122.Views:171In spite of the several good properties of peat, recently, some experiments were carried out with the aim of finding natural materials which can substitute for peat. According to the results, several inorganic and organic materials were proved to be suitable for this purpose. This study examines the effect of different organic materials (example: pine bark, composts, peats) on the growth and yield of green pepper (Capsicum annuum L., variety Danubia). We found that the most developed plants were grown in peat-mixtures and pine bark. The average fruit weight was the highest at those plants which were planted also in these media. The plants which were grown in composts fell short of our expectations in development and in yield, too.
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The effect of cluster thinning, cluster tipping, cluster shredding and defoliation at the flowering on the vegetative and generative vine performance from Kékfrankos Cv.
63-68.Views:410The cluster thinning is a method of the yield regulation.With the removal one part of the clusters, the yield pro leaf area will be lower, hereby the grape and wine quality will be improved. The regulation of the yield can lead to further advantages: the ratio of the vegetative and generative performance of the vines will improve, the condition of the plants will better, the number of the physiological diseases can be reduced and the growth of the shoots and roots can be promoted. The grape growers make the cluster thinning almost exclusive by creating one cluster shoots. Usually the upper clusters are removed, because the sugar content of these second or third clusters will be lower. The cluster thinning is an easy task, can be done without special skills. It is an effective method improving wine quality, but its use can lead to other problems. The grapes try to compensate the removed clusters. Therefore the clusters will be bigger and thicker, but more sensitive to bunch rot. Moreover the treatment is expensive, because it needs manual work. It is worth to get acquainted and try the new yield regulation methods, which can help to avoid the occurring problems. Our aim is to show the results of our experiment, which was carried out in Eger, examining the red grape cultivar Kékfrankos. During our 4 years long experiment we compared the effects of cluster thinning, cluster shredding, cluster tipping and defoliation at the flowering, on the vegetative and generative vine performance.
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Anatomical relations of root formation in strawberry
71-75.Views:416Anatomical relations of root formation are traced throughout the life cycle of the strawberry plant from the germinating seed up to the runners of the adult plant. Histological picture of the root changes a lot during the development of the plant. First the radicle of the germ grows to a main root, which makes branches into side roots and later adventitious roots are formed on the growing rootstock or rhizome. The anatomy of the different types of roots is also conspicuously different. First tiny branches appear relatively early after germination on the seedling's radicle, but soon the hypocotyl of the seedling thickens and develops side roots, which are already somewhat stronger. During this interval, the first true leaves are formed. The 4th or 5th of them being already tripartite, and the initiation of new roots extends into the epicotylar region of the shoot. The second years growth starts with the development of reproductive structures, inflorescences and runners starting from the axils of the new leaves. Near the tips of the runners below the small bunch of leaves, new root primordia are initiated. The tiny radicle of the germ develops a cortical region of 5-6 cell layers. Cells of the central cylinder are even smaller than the cortical parenchyma and include 3-4 xylem and 3-4 phloem elements as representatives of the conductive tissue. Roots originating from the shoot region are much more developed; their cortical zone contains 17-20 cell layers, whereas the central cylinder is about half as large. In the next year, new roots are formed at the base of the older leaves. These roots differ hardly from those of the last season in size and volume, however, they are recognised by colour and their position on the rhizome. The roots of the last year are dark, greyish-black, and grow on the lower third length of the rhizome, on the contrary, the new ones, on the upper region, are light brown. Roots starting from the shoot or rhizome are, independently from their age or sequence, mainly rather similar in size and diameter, thus being members of a homogenous root (homorhizous) system, i.e. without a main root. Plants developed and attained the reproductive phase develop in the axils of the leaves runners being plagiotropic, i.e. growing horizontally on the surface of the soil. The runners elongate intensely, become 150-200 mm, where some long internodes bear a bunch of small leaves and root primordia on short internodes and a growing tip. Runners do not stop growing, generally, further sections of 15-25 cm length are developed according to the same pattern, with small leaves on the tip. The growing tip of the runners is obliquely oriented, and small, conical root primordia are ready to start growing as soon as they touch the soil. The roots penetrate the soil, quickly, and pull, by contraction, the axis of the runner downwards, vertically, developing a new rhizome. The short internodes elongate a little and start developing adventitious roots. At the end of the growing season, the plantlets arisen on the rooted nods of runners are already similar to the original plants with homogenous root system. On the side of the adventitious roots, new branches (side-roots) are formed. The root-branches are thinner but their capillary zone is more developed being more active in uptake of water and nutrients. The usual thickening ensues later.
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Effect of water supply on nutrient transport in grapevine varieties
64-68.Views:236The effect of water supply on availability of macro nutrient elements (N, P, K) by the plants in the soil and their transport in the plants were examined. In a field experiment two grapevine varieties characterized by higher (White Riesling B 7) and lower (Kövidinka K 8) water requirement were compared on the basis of N, P, K concentrations of leaf blades, petioles and berries. A different water supply of the vineyard was achived by striped coverage of the soil with plastic foil to exclude rainfalls from the beginning of May to ripening. Humidity of the soil decreased as the vegetative phase advanced. Soil cover resulted in 25-30% decrease of the water content in the soil at flowering but this difference gradually disappeared till veraison (i.e. the start of intense growth of the berries). The water consumption in the White Riesling B 7 plantation was more intensive. Mobility and availability of N, P, K in the soil was restricted by water exclusion (i.e. plastic soil cover) at flowering. Nitrogen was slightly affected, whereas P and K were in a higher extent. Comparing the transport of nutrient elements in the two varieties, leaf blades of Kövidinka K 8 contained less N and more P and K than White Riesling B 7 at flowering and more N and P and less K at ripening. Water deficiency inhibited K accumulation in the berries of White Riesling B 7, while this effect did not appear in Kövidinka K 8.Water exclusion decreased the yield of White Riesling B 7 already at lower bud loading, the yield of Kövidinka K 8 was affected only at higher bud loading. The higher yields of the treatments in Kövidinka K 8 plantation support the superior performance of this variety under the hot and dry climate of the Hungarian Great Plain.
