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Detergent induced pollen sterility in some vegetable crops
85-88.Views:133Efficacy of a popular synthetic detergent, Surf excel in some important vegetable crops viz. Okra or lady finger (Abelmoschus esculentus L.), chilli or red pepper (Capsicum annuum L.) and tomato (Lycopersicon esculentum Mill) was evaluated for inducing male sterility and hybrid seed production. Foliar sprays with aqueous solutions of Surf excel (1.0 and 1.5% w/v) in these crops induced complete pollen sterility. The treated plants showed a delay in flowering, a reduction in the number of flowers and fruits/plant, number of seeds/fruit resulting in a reduction in yield/plant. However, the male sterility thus induced was successfully exploited for hybrid seed production.
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Production of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) gene
104-107.Views:167Dianthus chinensis and Dianthus caryophyllus varieties were tested for shoot regeneration from leaf and petal explants and transformed with Agrobacterium tuniefaciens strains (EHA 105 and LBA 4404) harbouring an apple derived ACS cDNA in antisense orientation in order to reduce ethylene production and influence the ethylene dependant traits in carnation. After transformation regenerating shoots were selected on MS medium containing 50-75-100-125-150 mg/1 kanamycin and supplemented with 1 mg/1 BA, 0.2 mg/1 NAA. Transgene integration was proved by PCR analysis with npt II spcific primers followed by Southern hybridisation of DNA isolated from green shoots on medium containing 150 mg/1 kanamycin. Several putative transformants were subjected to RT-PCR in order to examine the npt 11 expression at mRNA level. Both the transformant and the non-transformant plants were potted into glasshouse to observe the effect of changed ethylene production on flowering time, petal senescence and vase life.
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Effect of pruning intensity on performance of apple cultivars in environmentally friendly production systems
65-67.Views:199The two worldwide accepted environmentally freindly production systems are integrated and organic. The two systems shows considerably differences in several practical production technology elements. These effects come forward in generative processes (e.g. flowering, fruit setting) and vegetative features (e.g. growth). The aim of our study was to study the effect of pruning intensity on growth features of cultivars in integrated and organic apple production. In early spring of 2009, we determined two pruning intensities (strong and week) on six scab resistant and tolerant cultivars. Strong pruning resulted in stronger shoot growth for all cultivars compared to weak pruning. It is obvious that pruning intensity had more effect on vegetative performance than conditional differences originates from integrated and organic production.
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Isolation of living sperm cells and in vitro fusion of Torenia fournieri gametes
81-85.Views:107In contrast to most angiosperms, Torenia contains a naked embryo sac and therefore has been considered since many years as an exciting model plant to study the double fertilization process of flowering seed plants. It is thus not surprising that the isolation of protoplasts from the female gametophyte has been reported already 20 years ago by Mol, the isolation of megaspores and megagametophytes has been published by the authors of this manuscript in 1996 and in 1999. The isolation of the male gametophyte and of sperm cells was published by the authors in 2004. The isolation of viable Torenia sperm cells is a crucial part of the elaboration of an in vitro fertilization system. Torenia sperm cells were isolated from in vivo — in vitro cultured pollen tubes. In this system pollen tubes first grow inside a cut style then follow their elongation in a solid isolation medium. The medium contained agarose in order to detain pollen tube contents. Released sperm cells and enzymatically isolated egg cells were collected and handled using glass micropipettes and transmitted to an electrofusion apparatus or polyethylene glycol containing media for fusion probes.
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Effect of 1-MCP (1-methylcyclopropene) on the vase life of Chrysanthemum and Carnation cut flowers
29-32.Views:209The effect of 1-MCP on extending the vase life of chrysanthemum and carnation cut flowers was studied. The flowering stems of both flowers were terminated to 50 cm. in height. Then, the flowers were pre-treated with 1-MCP at 0.3, 0.5 and 0.7g/m3 for 3 hours or 6 hours. The control flowers were placed in ambient air during the treatment. After the period of treatments the flowers were aerated then put in glass vials contained tap water. The vase life determination was conducted in a vase life evaluation room at 22 ± 1°C. Fresh weight determinations of the flowers were made just before the immersion of the flowers into the glasses of water and were repeated on the day when the vase life of the control flowers was terminated. The treatment of 1-MCP at 0.5g/m3 for 6 hours was the most effective treatment of chrysanthemum and carnation cut flowers.
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The effect of the limitation of insect pollination period on the fruit set and yield of temperate-zone fruit tree species
90-95.Views:141The duration of effective bee pollination period was limited by caging flowering branches for shorter or longer time in blooming fruit trees in a number of experiments during the past decades. In the case of self-sterile fruit species and cultivars (apples, pears, quinces, some plums, some sour cherries) even partial limitation of the effective duration of bee pollination period significantly reduced the fruit set and the yield. In the case of self-fertile apricots the effect of the total and also the influence of partial limitation of bee pollination period was the same as in the case of the mentioned self-sterile fruits. On the other hand, in the case of another self-fertile fruits (some plums, some sour cherries), the effect of partial limitation of bee pollination period was usually small, but complete (or incomplete but strong) limitation of be pollination usually resulted in a strong reduction of yield. This means that not only self-sterile but also self-fertile fruits clearly depend on insect (bee) pollination. This is because pollen dehiscence of anthers and the receptive period of stigmas do not overlap in time within the individual flowers. Stigmas in self-fertile trees, therefore, need pollen carried by bees from another flowers of the same tree (or compatible pollen from another trees). Accordingly, additional bee pollination (moving bee colonies to the orchards in flower) is needed to all kinds of temperate-zone fruit tree species when bee visitation of plantations is not abundant enough for some reasons.
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Impact of boron foliar fertilization on annual fluctuation of B in sweet cherry leaves and fruit quality
27-30.Views:268The goal of the study was to examine response of sweet cherries (Prunus avium L.) to boron (B) fertilization. The experiment was conducted during 2005-2007 in West Hungary on mature cv. `Germersdorfi 3' grafted on Prunus mahaleb rootstock.
Sweet cherry trees planted on a calcareous chernozem soil. Trees were foliar-fertilized with B. Foliar B sprays were performed: (1) in the spring, at the stage of white bud, beginning of flowering (B1), and (2) repeated 5 weeks after full bloom (B2). In each of spring spray treatments, B was applied at a rate of 0.15 kg ha-I. Trees untreated with B served as a control.
The results showed that B fertilization had effect on B concentration in leaf tissues, mostly after ripening. B was present significantly higher amount in leaf in treated samples after ripening.
Mean fruit weight was slightly increased by B fertilization. Fruit sensitivity to cracking was not influenced by B fertilization. Nevertheless, from our data it can be conclude that the sensitivity of fruit to cracking is improved when the fruit is riper, the fruit density and fruit weight are higher. The soluble solids varied between 15.0 and 15.9% according to the treatments. Our results for the monosaccharides investigated varied between 5.1 and 7.2 as glucose and fructose as well. Galactose and sucrose was detected very small amount in the unprocessed cherries. Applied B treatments increased sugar contents but decreased organic acid contents in sweet cherry fruits.
It is concluded that under conditions of this experiment, B fertilization can be recommended in sweet cherry culture to improve fruit quality and their appearance.
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Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
69-77.Views:93The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.
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Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis
69-72.Views:147Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of some Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S1S2) and `Harcoe (S1S4) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.
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Fruit set and yield of pear cultivars as affected by reduced bee pollination period
11-16.Views:179Results of our experiments prove that pear is more or less sensitive to the reduced bee pollination period. However, the reaction (or the sensitivity) of cultivars may be different to the reduced bee pollination. Most cultivars produce much less yield under reduced bee pollination or no yield with the exclusion of bees but in the case of some cultivars total exclusion of bees does not prevent the yield formation and what is more sometimes reduced bee pollination can be resulted in somewhat higher yield than open pollination. Typical reaction, however, is a significant yield reduction with reduced bee pollination. Pear seems to be somewhat less sensitive to the partial reduction of bee pollination period than apple or quince. The first half of the flowering period seems to be more important in yield formation because usually higher yield was resulted when pear cultivars received open pollination in the first than in the second half of the blooming period. Based on our experimental results no definite relationship between parthenocarpic capacity of cultivars and the yield under reduced bee pollination can be established. So reduced bee pollination does not seem to contribute the parthenocarpic fruit formation in pear.