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The Role of Trichoderma in the Rhizosphere of Tomato Plants
Published May 11, 2003

It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been not...ed for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains.

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Application of AquaCrop in processing tomato growing and calculation of irrigation water
Published June 30, 2018

The area and volume of processing tomato production is increasing in Hungary. Irrigation is crucial for processing tomato growing. To save water and energy, it is important to know exactly how much water is needed to reach the desirable quality and quantity. AquaCrop is a complex software, developed by FAO, which is able to calculate irrigation... water needs, several stress factors and to predict yields. A field experiment was conducted in Szarvas in processing tomato stands, under different irrigation treatments. These were the following: fully irrigated plot with 100% of evapotranspiration (ET) (calculated by AquaCrop), deficit irrigated plot with 50% of ET (D) and control (K) plot with basic water supply was also examined. Dry yield, crop water stress index and soil moisture were compared to modelled data. The yields in the plots with different access to water were not outstanding in the experiment. The model overestimated the yields in every case, but the actual and modelled yields showed good correlation. AquaCrop detected stomatal closure percentages only in the unirrigated plot. These values were compared to CWSI – computed from leaf surface temperature data, collected by a thermal cam in July – and showed moderately strong correlation. This result suggests that Aquacrop simulates water stress not precisely and it is only applicable in the case of water scarcity. Soil moisture data of the three plots were only compared by means. The measured and modeled data did not differ in the case of K and ET plots, but difference appeared in the D plot. The obtained results suggest that the use of AquaCrop for monitoring soil moisture and water stress has its limits when we apply the examined variables. In the case of dry yield prediction overestimation needs to be considered.

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Evaluation of nutrient conditions in open hydroponic system based on tomato production
Published November 15, 2007

Monoculture caused a gradual decline of soil conditions, while nematodes and salt accumulation stimulated the growers to choose alternative practices, such as soilless cultures, which proved their value in Western Europe. Exact statistics are lacking, but estimates deal with approximately 300-400 hectares of vegetable on rock wool, whereas othe...r substrates of soilless culture may multiply this number. Real perspectives are attributed to the forced production of pepper, tomato and cucumber.
Vegetable production in greenhouses may impair the ecological balance of the environment substantially as far as being uncontrolled. Soilless cultures especially should be handled thoughtfully. A fraction of the nutrients administered, more than 25-30%, is doomed to be lost in an open system, and the resulting ecological risk is accompanied with increasing costs of the production.
In Hungary, the quantity of nutrient elements in drainage water is unknown, et all. Connecting the production results with chemical analysis, we gain more information about it.
You can see a mathematical method for evaluation of nutrient and water conditions in tomato hydroponics production.

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Rheological evaluation of industrial tomato
Published December 15, 2019

Today, tomato has the second largest harvesting area globally, and the fourth largest in Hungary. The importance of industrial tomato is growing year by year, and one of the most important tasks is to satisfy the needs of the industry. To meet these needs, the producer has to find the proper genotype, which can achieve up to 100 t ha-1...p> crop yield. This quality has high importance to be researched.

The experiment was carried out in the Botanical and Exhibition Garden of the University of Debrecen, with 3 examined genotypes. The planting date was 3 of May 2018. The harvesting was two-threaded. On 27 of July the physical parameters – fruit shape index (length/diameter), firmness (kg cm-2), water-soluble dry matter content (Brix%) and weight of the berries (g) – were evaluated. On 15 of August the rheological parameters were examined – the force needed to tear the skin – bioyield point (g) and flesh firmness (g).

The fruit shape index was oval (between 1.19 and 1.24) for all the examined genotypes. The firmness was between 0.92 (UG1122713 F1) and 1.05(Prestomech F1) kg cm-2, which compliance the needs of the industry. The water-soluble dry matter content was suitable, with a value between 4.49 (Prestomech F1) and 4.65 (UG1122713 F1) Brix%. The weight of the berries was between 45.94 (UG989 F1) and 49.37 (Prestomech F1) g per piece.

The bioyield point was between 76.00 (UG1122713 F1) and 85.16 (UG989 F1) g. The flesh firmness was between 33.68 (UG1122713 F1) and 42.81 (UG989 F1) g.

Finally, we can conclude based on the obtained data that the UG989 F1 hybrid was the most proper for the applied cultivation conditions. This genotype can be recommended for the farmers even in extreme weather conditions.

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Biomass production estimation of processing tomato using AquaCrop under different irrigation treatments
Published December 15, 2019

The wiser usage of irrigation water is inevitable in the future. Irrigation has very high input cost; therefore, farmers must carry out irrigation with care. Also, the effect of irrigation on crops has a big role in decision making. Modeling provides a possibility to evaluate this effect. AquaCrop, as a crop production simulation model has grea...t potential in this field. The accuracy of tomato biomass yield prediction of the model was tested in this research. For collecting the necessary data, a field experiment was conducted at Szarvas on processing tomato with different water supplies, such as 100% (I100), 75% (I75), 50% (I50) of potential evapotranspiration and a control with basic water supply (C). The relation of the simulation and actual biomass yields was evaluated during the season. Very good correlation was found between the modelled and the actually harvested data. The data for the control and I100 treatments showed higher correlation than the I75 and I50. The relationship for all of the data was moderately strong. Miscalculations occur mostly when the dry biomass yield reaches
7 t ha-1. The accuracy of the model was evaluated with the use of mean absolute error (MAE) and root mean squared error (RMSE) values. The least error was found in the C treatment, which means 0.34 MAE and 0.45 t ha-1 RMSE. The simulation resulted in higher errors in the I75 and I50 treatments.

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The effect of water supply for yield formation of processing tomato
Published November 13, 2012

The effect of irrigation on tomato yield depends on the actual weather conditions, basically the air temperature and precipitation (quantity and dispersion). The irrigated plants gave a significantly higher yield, and rainfed plants showed a yield loss. The optimum water supply treatment gave a significantly higher proportion of lycopene than t...he control (rainfed) treatment (89% and 80% respectively) in case of Brixsol F1 variety. In spite of this we have not found significant difference in case of Strombolino F1 (cherry type). Content of total poly-phenols measured in Strombolino F1 (cherry type) was significantly higher than that found in the Brixol F1 (normal type). The optimum water supply treatment resulted in a significantly lower content of total polyphenols in both years. Tomato fruits are rich in polyphenols the largest amount of which is rutin from among the identified components.

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Improved soil and tomato quality by some biofertilizer products
Published September 5, 2018

The use of microbial inoculums is a part of sustainable agricultural practices. Among various bioeffectors, the phosphorus-mobilizing bacteria are frequently used.

The objective of this study is to investigate the effect of some industrial biofertilizer inoculums, of containing P-mobilizing bacteria on the quantity and some quality para...meters of tomato fruits. Spore-forming industrial Bacillus amyloliquefaciens FZB42 (Rhizovital) as single inoculums and combinations with other Bacillus strains (Biorex) were applied on Solanum lycopersicon Mill. var. Mobil test plant. Soil microbial counts, phosphorus availability, yield and fruit quality, such as total soluble solids (TSS) content and sugars (glucose, fructose) were assessed. The results found that single industrial inoculums of FZB42 product had positive effect on P-availability and fruit quality in the pots. Fruit quality parameters, TSS content, soluble sugars were significantly improved (p<0.05). Such better fruit taste was correlated significantly by the most probable number (MPN) microbial counts. Use of such bioeffector products is supported by the positive interrelation among measured soil characteristics and inside healthy quality parameters of tomato fruits.

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The effects of water supply on the physiological traits and yield of tomato
Published December 15, 2019

The SPAD value, the chlorophyll fluorescence and the canopy temperature of the leaves and fruits of tomato hybrid “H1015” were investigated under non-irrigated (I0), deficit irrigated (I50) and well-irrigated (I100) conditions. The aim of the experiments was to show which treatment effect on the examined traits affected photosynthesis, leaf... temperature and yield quantity, as well as quality under water scarcity. In the control treatment (I0), the canopy temperature increased, but the SPAD decreased compared to the other two treatments (I50 and I100). Chlorophyll fluorescence produced a fluctuating result. In the end, the number of the fruits was high, but the number of the sick and green berries was increased.

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Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary
Published November 10, 2010

Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last deca...des. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.

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Fruit melanotic ringspot (FMRS) – a disease of resistant Capsicum genotypes infected with Tomato spotted wilt virus (TSWV) on the fruits
Published October 30, 2011

Etiology of pepper fruit melanotic ringspot (FMRS) disease (Salamon, 2009) was studied on fruit samples collected in forced pepper populations. It was noticed that in spite of heavy thrips (Frankliniella occidentalis) infestations and of TSWV epidemy detected in the forcing houses, FMRS occurred only in plants having healthy foliage. Symptomato...logical surveys strongly suggested that FMRS appeared exclusively in specific pepper genotypes. The size of melanotic ringspots has been observed to grow at room temperature during postripening of diseased fruits. A mechanically transmitted plant virus was isolated from symptomatic parts of 9 white pepper fruits affected by FMRS. On test plants each of the virus isolates caused systemic symptoms characteristic to TSWV. Using cDNA/PCR technique and TSWV N-gene specific primers a ca. 300 bp long DNA fragment has been amplified from total nucleic acid extracted from symptomatic tissues but never from asymptomatic parts of the fruits showing FMRS. Plant progenies grown from seeds of FMRS diseased fruits segregated in respect of resistance and/or susceptibility to TSWV infection. TSWV was also detected in and isolated from three fruits showed non-melanotic yellow rings (one of them was infected with a tobamovirus, too). Seedlings derived from these fruits proved to be susceptible to TSWV. Based on the above results we could conclude that the FMRS disease developed on fruits of “cecei” type white peppers that carry a TSWV resistance gene, most likely the Tsw gene in heterozygous form. These fruits were infected with thrips transmitted TSWV and FRMS appeared as a hypersentive reaction (HR) manifested in fruits.

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Microbiological and Chemical Characterization of Different Composts
Published May 11, 2003

Composting of agricultural waste is considered particularly important from the point-of-view of environmental protection. Degradation of organic substance results in a significant reduction of waste volume.
The end product of the composting process, mature compost, can be used as soil coverage against excess loss of wastes, for mulching, for... organic manure etc. The problem of composting has come into limelight in environmental studies and in agriculture.
The quality of the mature compost is determined by physical, chemical and biological parameters of the composting process which, in turn, depend on initial composition of the raw materials, the technology, e.g. regular mixing and moistening and on environmental factors. Quality is the key question in compost use.
We studied the composting process in compost windrows of different raw material composition. We measured temperature, humidity content, pH, organic substance content, nitrogen and carbon content.
We counted the number of bacteria, microscopic fungy, ammonifying and cellulose decomposing microorganisms. We directed the composting process with turning weekly (to provide oxygen) and watering (to provide humidity content 40-60%).
We set up windrows of 1 m3 volume from dry plant substances (cornstalk, pea straw, tomato stalk and crop, weeds) and cow manure not older than 1 week. The cow manure was used at ratios of 0%, 35%, 50%, 65% and 100%, respectively.
We measured changes in compost temperature relationship with outside temperature until they were almoust the same. Humidity was 40-60% in most cases.
At the beginning of the process, pH was slightly acidic-neutral; it later becomes neutral-slightly alkaline (pH: 6.93-8.02) as ammonia is liberated from proteins.
At the end of the process, pH decreased again, due to humification.
Organic substance content decreased as microorganisms mineralized them. Organic carbon content decreased gradually due to microorganisms used it as an energy.
Total nitrogen content increased until middle of july and decreased gradually until than.
The carbon/nitrogen rate were higher in the beginning, it decreased until july-august and increased by smaller degree until end of the process.
The number of bacteria was higher in the first three weeks and between june-september. The number of cellulose degrading bacteria was the highest in the first three month, the number of ammonifying bacteria was the highest from the end of may until sepember.
The number of microscopic fungy was significant in the second part of process, after july.

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