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Correlation between body condition change and reproduction parameters of Holstein-Frisian cows after calving
49-52Views:101The authors examined the body condition with relation to reproduction of Holstein-Friesian cows on three dairy farms in the South Plain region. During the examination they analyzed the data of 516 births of 494 Holstein-Friesian cows. They were trying to find correlation between the first service, the service period and the service rates. The examinations with regard to the correlations were different from the ones published in the professional literature. The body condition at the time of calving affected the interval from calving to the first service but not the conception
rates or the service period. The examinations did not prove any correlations between body condition changes and reproduction. -
Effects of diets with different fibre content on the performance of rabbit does and on parasitological infection
41-44Views:91The effect of two diets with different fibre contents was examined on rabbit does’ performance and parasitological infection. Diet P2 had 1% higher crude fibre, ADF and starch contents than that of diet P1. The performance of 12–12 does and litters per group were examined during two consecutive inseminations. In both dietary groups, according to a 49-day reproduction rhythm, the does were artificially inseminated 18 days after kindling. Most production traits were not affected by the diets (kindling rate, weight of does and their kits, feed intake, mortality; P>0.05). Significant differences were only found in litter sizes as the number of kits was lower in P1 diet fed group at all examined days of lactation. The significant “Diet x Reproduction cycle” interaction showed that the litter sizes of dietary groups did not differ at the first examined insemination, however at the subsequent reproduction cycle 6–8% lower litter sizes were counted in P1 fed does compared to group P2 (litter size at 4 day: 9.00 vs 9.58–9.92, P = 0.004; litter size at 11 day: 8.83 vs 9.58–9.92, P = 0.037 for diet P1 at the 2nd reproductive cycle compared to all the other cases, respectively). The two diets with different compositions did not affect most of the production results of the rabbit does and their kits, but the lower litter size of does consuming P1 diet at the second examined lactation suggests the adverse effect of P1 diet’s long-term use. From the collected manure samples not any parasites were detected which presents a very favourable picture of farm from the point of view of hygiene and animal health.
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Some Practical and Biotechnological Methods for Improving Reproduction Traits in Sheep
15-20Views:118However, reproduction in sheep is seasonal, many breeds of sheep are able to mate not only in autumn, but out-of-season as well. The main factor determining seasonality is the photoperiod, but other factors can influence reproductive pattern, such as genetics, management practices and social cues. The fertility of spring and early summer breeding is usually lower; this imposes the need for alternative methods (e.g. hormonal treatments, biotechnological practice), to increase the conception rate.
The author summarize the main practical techniques and biotechnological methods for controlling reproduction completed with some own experimental results in connection with different topics. -
Effect of different n-6/n-3 fatty acid proportion oil sources on reproduction performance and fatty acid profile of milk in modern genotype sows - Pleminary results: Preliminary results
121-128Views:144This study was conducted to investigate the effects of supplemental n-6 and n-3 fatty acids on sow’s milk fatty acid composition during the lactation period and on reproductive efficiency of sows in the subsequent gestation period. Data were collected on a total of 213 DanBred sows (108 control and 105 experimental) representing parity of 2–7, respectively. Control and experimental sows were placed in the same housing conditions during lactation and gestation period. Control group received 6.3 g of sunflower oil (SO) per kg feed as n-6 fatty acid supplementation, whilst experimental animals received the same amount of fish oil (FO) as n-3 fatty acid source. Diets were corn meal-extracted soybean meal based. The experiment was conducted in one replication as being a part of a longer and more comprehensive trial. It was found that the consumed long-chain polyunsaturated fatty acids (LC-PUFAs) appeared in the sow’s milk and changed its fatty acid profile. With this alteration, the n-6/n-3 ratio of experimental (FO) sows’ milk were narrower than in SO group (SO: 13.82 vs. FO: 5.89). The benefits of n-3 fatty acids supplementation were evident for the subsequent reproduction cycle, when experimental sows heated more reliable and earlier than control (weaning to oestrus interval: SO: 5.86 vs. FO: 4.48 days). Only 2.33% of experimental sows (FO) did not heat within 7 days after weaning, but this was 12.36% in the control group (SO). The present study requires further research to evaluate the effect of n-3 fatty acids on maintenance of pregnancy and improved subsequent litter size.
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Examination of Reproductive Performance of Roe Deer (Capreolus capreolus) in Hungary
33-38Views:101The objective of the research partly is to compare the reproduction performance of the populations living in different regions with regard to some special characteristics (age, condition).
When estimating the age through tooth wear and cementum-layer-counting there was a difference of 0.87 years in favour of the first one (r=0,840; p<0,001). I found cementum layers at 42% of the does in the study after examining the MI teeth.
There was lose connection between the weight (eviscerated, with head and legs) and the KFI (r=0,296; p<0,01), and for further analysis, I used only the KFI as the index for condition.
The regional average KFI varied from 0.24-0.37 in fawns, 0.82-1.73 in does, with individual extremes of 0-4.05. Within the examined regions the highest index belonged to the prime-aged does, while the 1-year-olds had a lower rate, and it was the lowest in the does older than 8 years.
The rate of fertility was between 83,3(ns)-100% as we can see from the presence of the CL. All the examined does were fertile, except in one region, while among the female fawns in two regions I only found three with active ovaries. The average number of CL was 1.5-2.13, and this varied by regions; all in all it was the highest in the 2-7-year-old group (1.96) and in the ones over 8 years (2.00!), while it was lower in the does younger than 1 year (1.90). The high fertility of the does over 8 years is remarkable.
I could examine the number of embryos in two regions during the post-implantation period, and beside 100% fertility I found significant differences among the does, which can be associated with the condition. The ratio of CL carriers and the pregnant does was 100% and 73% in the two regions, the average number of CL were 1.92 and 1.72, while the average embryo number were 1.83 and 1.36 per doe. The difference between the CL and the embryo numbers on the two regions were 5% and 21%. The difference (prenatal loss) is in connection with the age (age class) of the doe. It is possible, however, that in some cases oestrus was not followed by gestation. But in roe deer, owing to the commonly known lack of luteolysis-mechanism (Flint et al., 1994), the regression of the CL of the does that did not get pregnant takes place in December and January, so the CL found in January cannot prove a previous pregnancy, which might have been followed by an abortion.
Although it has to be proven, it seems that the number of the CL (potential progeny) can be associated with the age (r=0,418; p<0,01) and the weight (r=0,312; p<0,01) of the doe, while the embryo number (realised progeny) is influenced by the age of the doe and probably by external factors.
It is essential to continue and extend the research to increase the reliability of the results and their correlation. -
Studying the reproduction-biological effect of the GPR54 receptor gene in sheep
48-51Views:94One of the main economical problems of sheep breeding is that continual production is not possible due to the seasonality of animals. Today, genes, mutations that may develop aseasonality in sheep are extensively researched in order to make the establishment of populations capable of aseasonal estrus possible.
According to the current knowledge, the GPR54 gene participates in the GnRH release from the hypothalamus, and thus in the alteration of the reproduction state of the organism. Our research is aimed to determine whether the GPR54 gene has allele variations that influence the proneness to aseasonal estrus in sheep in a different extent. Therefore, the GPR54 gene sequence of sheep is first examined. -
Matériel et conditions de reproduction du ver à soie de chêne Antheraea pernyi Guer (Bombycidae)
58-60Views:59In this study we highlighted the conditions that need to be fulfilled in order to have successful oak silkworm reproduction.
Thus, conditions for best cocoon hibernation should be as follows: strata of 10-15 cm with optimal temperature around 0oC, relative humidity of 60-70%. The cocoons must be moved every 10 days and must also be daily aired.
Butterfly mating should take place either the night of their coming out or the second day. It takes 14-15 hours; male butterflies outnumber females by 10%.
After mating, the optimal temperature for laying eggs is 18-20oC; relative humidity is 80%; egg laying takes place in the dark. -
The role of type traits of dairy cattles in long productive life
11-18Views:105In the last few decades, a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Additionally, the reproduction biology of the population was adversely affected by keeping and feeding technologies. Productive lifetime has shortened and the service period has lengthened. However, profitable milk production requires cows with longer productive lives and larger life-time productions. In our study, we made a comparison between production, culling causes, and type traits of culled Holstein Friesian cows after first calving and of cows with a minimum of eight lactations. We established that the first lactation production of cows with longer productive lives was more or less homogeneous. Disease of metabolism as well as digestive and respiratory problems, were the main causes of the culling of cows with short productive lives, and reproduction and udder problems as well as low production, were the main causes of the culling of cows with long productive lives. We found a significant difference in the distributions of strength, body depth, dairy form, croup width, rear leg side view, foot angle, fore udder attachment and teat placement between the two groups. Furthermore, we established that cows with lower stature, less strength, a somewhat deeper body, better dairy form, narrower croup width, smaller foot angle, looser fore udder attachment and some outside teat placement had longer productive lives.
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Results of Brown Bullhead Fingerling Rearing in Recirculating Fish Production Systems
46-50Views:94Predator fish species are in great demand, both in fish production and consumption. Because of their rarity and perfect meat quality, these species have considerable economical significance and increasing domestic and foreign demand.
The brown bullhead (Ictalurus nebulosus) has been in Hungary for more than 100 years, but not popular because of its early maturation rate, aggressive reproduction and slow growth. Despite of these properties, larger specimens (about 150-200 g) are marketable overseas.
Our objective is to ensure the adequacy of the brown bullhead in intensive fish farming and its biological, technological and economic references. The pre-experiments were performed at the Bocskai Fishing Co-operative in Hajdúszoboszló, where we reared the juvenile age class. In our university’s recirculating system we examinated the brown bullhead from the fingerling age class.
The black bullhead prefers artificial feed, and produces excellent growth parameters and a specific feed-live weight conversation ratio. -
75 years of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr in Europe
82-85Views:197The chestnut blight fungus Cryphonectria parasitica is a native pathogen in East Asia and has been introduced into North America and Europe. Historical records and population genetic studies revealed at least three major introduction events from Asia into Europe.
Nowadays, chestnut blight is present in almost the entire distribution range of European chestnut, i.e. from the Iberian Peninsula to the Caucasus. The C. parasitica population in most countries has been studied in respect to the diversity of vegetative compatibility (vc) types and the occurrence of hypovirulence. The vc type diversity of the different populations varied considerably. Typically, a high diversity of vc types has been found in areas with a long history of chestnut blight and where sexual recombination between divergent genotypes commonly has occurred. On the other hand, newly established populations often showed a low diversity with only one, or a few vc types present.
Hypovirulence, i.e. the occurrence of C. parasitica isolates infected by Cryphonectria hypovirus 1 has been found widespread in Europe. Natural dissemination and active biological control applications have lead to a high prevalence of the hypovirus and to the recovery of many chestnut stands. Virulent cankers became hypovirus-infected within a short time and ceased expansion. There is concern that the diversity of vegetative compatibility types could increase in Europe through sexual reproduction between C. parasitica genotypes originating from different introductions. A higher level of vegetative incompatibility would not only hamper hypovirus spread within a population but could also select for lower virulence in CHV-1 and subsequently lead to an erosion of biological control. Recent studies, however, indicate that the vc type barriers are not so restrictive than previously assumed and that so far no evidence for an erosion of biological control system in high diversity populations can be observed.
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Complex assessment of inland water fish stocks
74-80Views:80In the domestic fish production, natural waters have yielded for several years about 7-8 thousand tons. This, from the point of view of outputs, considering the almost 130 thousand hectares of natural water, is rather low, it means approximately 55-60 kg/ha mixed fish.Although the various natural waters can differ significantly on the basis of yields, yet on the majority of the territories, the results were low. In the case of our extensive still waters and rivers, the reason can undoubtedly be found in the combined effect of the lack of the possibility of reproduction of the fish stock and the over-fishing. Fishery built on planning supposes the best possible knowledge in the given circum stances of the parameters of the water area and its fishstock. Lacking this knowledge, it is not possible to establish the optimal use fulness of the resources, what is more, the management can make faulty decisions – as a result of a lack of information -, which can risk the success of later activities.
It is known that many factors have an impact on the success of the fishery, as well as some information in connection with the water area and the fish stock are necessary, the knowledge of which make it possible to manage the fishery in a planned way. One part of the information is available, while the other part is incomplete or not deep enough. The necessary data are dissimilar depending on their nature, can be obtained from different places, by different methods.
As the first step for executing the field surveys and processing data, I developed a complex model, which contains in a unified system the steps of estimating the fishstock. I made the sampling on the basis of this. Part of the model is a fish faunistic survey, as well as a morphological survey of the water area. The information gained from these are important for making more accurate the system of devices of the samplings for stock estimation (duration, number of net-rows) and for assigning its place (places representing the best way the physical characteristics of the given water area). The major stages of stock-survey: A) faunistic survey, B) physical survey of the bed, and C) sampling with the help of gill-nets. This is followed by the evaluation by the computer module.
The results of the research create a methodological and technical background for the fish faunistic and population biologic surveys still performed in different ways in our country, and by applying these methods together, all basic information about natural waters which help decision-making concerning fisheries can be obtaine deffectively. -
The effect of wet compost extract on the root tubers of green pea
51-54Views:142Nitrogen plays significant role in the life of plants, it could be the main limiting factor of plant growth. Sustainable plant nutrition pays attention to satisfy the plants’ nutrient demand without chemical fertilizers, e.g. by bounding the atmospheric nitrogen. The nitrogen fixing organizations play important role in supplying plants with nitrogenbecause the N2-fixingbacteria can fix high amounts of nitrogen.
Many effects of the sewage sludge compost extracts is known in the literature. We studied the effect of sewage sludge compost water extract in laboratory conditions on the growth of Rhizobium spp. isolated from green pea, while in a small plot experiment thepea-Rhizobium symbiosis were studied on sandy soil in the Nyírség region. The extract was produced under aerobic conditions. The compost extract was applied before and/or after sowing. In the laboratory experiments we used the sterile version of extract, in different doses.
In our work we present the effect of compost water extract on the number of green pea roots nodules, dry weight of the plant and reproduction of the Rhizobium bacteria. -
Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:95The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary
193-200Views:75The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.
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Usability of vegetable extracts in the protection against Alternaria alternata
113-116Views:139In our country, wormwood ragweed (Ambrosia artemisiifolia) may cause serious problems. Nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia), which is believed useless weed. Allergological point of view, most problematic weeds adventive. However, many physiologically very beneficial compound also included, those with the effects have been known also by the Indians. On this basis, herbs can be thought of as ragweed. Our goal was to present that the ragweed contains antifungal active substances as well. In this paper we tested the biological activity of the extracts against Alternaria alternata F.00750 in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Alternaria alternata. The minimum effective concentration was 300 mg extract in a Petri dish, which was three days inhibited the growth of fungus. Full fungicidal effect was observed over dose 525 mg.
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Clay-pit systems fishfaunistic research in the Middle-Tisza
81-92Views:72After the regulation of the Tisza River the chance of successful fish propagation lessened. Natural spooning places in the river almost completely disappeared. The fish have to find an adequate place for their propagation in the flood plain. The period of spooning usually coincides with flooding of the river. At this time fish try to find the flooded shallow places for spooning. These parts mostly include the clay-pits beside dams, which were accidentally established during construction of the dams. At this place the fry can find the necessary food. After decreasing the flood the fingerlings and a part of the spooners are trapped inclay-pits as these latter ones are not connected with the river bed. The clay-pits usually desiccate during the summer. The trapped fish population is eaten by water birds or harvested by the local man population. This means a great loss for reproduction of some fishspecies.
Within the framework of the Regional Rehabilitation Program at Nagykörű, supported by the “WWF Hungary” and a “SAPARD project,” these clay-pits became connected with each other in a stretch of 5 km, and they were jointed to the Tisza by a collecting channel. The water level has been regulated by a flood gate so that the water enters the holes during the flood and is released later on in to the water bed.
Data on fish were collected from the Nagykörű Whole System, from the Anyita pond and some isolated wholes in Szandaszöllős in 2004 and 2005. First of all fish fry and fingerling were collected and the success of spooning at these places was examined. Fry was harvested by a 60 x 80 cm sized lifting net of 2x3 mm mesh size. In other cases nets used by anglers for catching prey fishes was also used. Data were also collected from local fishermen who participated in saving the fry and fishing of Anyita pond.
Of the several thousand caught fish specimens 28 species were identified, and among them 5 protected and 5 economically important species was found. Protected fishes were as follow: gudgeon (Gobio gobio), bitterling (Rhodeus sericeus amarus), weather fish (Misgurnus fossilis), spined loach (Cobitis elongatoides) and tubenosed goby (Proterorhinus marmoratus). Among economically important fishessamples of asp (Aspius aspius), carp (Cyprinus carpio), wels (Silurus glanis), pike (Esox lucius) and pikeperch (Sander lucioperca) were -
Effects of water deficit on the growth and yield formation of maize (Zea mays L.)
143-148Views:157Maize (Zea mays L.) is the most important consuming cereal crop in the world after rice and wheat. This requires an understanding of various management practices as well as conditions that affect maize crop performance. Water deficit stress during crop production is one of the most serious threats to crop production in most parts of the world and drought stress or water deficit is an inevitable and recurring feature of global agriculture and it is against this background that field study of crops response to water deficit is very important to crop producer and researchers to maximize yield and improve crop production in this era of unpredicted climatic changes the world over.
A pot experiment was carried out to determine the effects of water deficit on growth and yield formation of maize. Two maize cultivars were used Xundan20 and Zhongdan5485. Three levels of soil water content were used in two stages of water control levels at two stages of the maize plant development
1. The JOINTING STAGE: A. CONTROL (CK) soil water content: from 70% to 80% of soil water holding capacity at the field, soil water content: from 55% to 65% of soil water holding capacity at the field, soil water content: from 40% to 50% of the Soil water holding capacity at the field.
2. The BIG FLARE PERIOD: A. CONTROL (CK) soil water content: from 75% to 85% of soil water holding capacity at the field, soil water content: from 58% to 68% of soil water holding capacity at the field, soil water content: from 45% to 55% of the soil water holding capacity at the field.
This research mainly studied the effects of water deficit on physiological, morphology and the agronomical characteristics of the maize plant at the different water stress levels.
The importance of these results in this experiment will enable plant producers to focus and have a fair idea as to which stage of the maize plant’s development that much attention must be given to in terms of water supply. -
Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
25-29Views:128In the last 20-30 years, lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimizing lameness. Those areas are related to poor facilities, lack of straw, problems with labor and basic management.
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Impacts of ethnoveterinary medicine integrated with livestock farm technologies on the performance and antimicrobial resistance of indigenous goats in South Africa –A review
93-103Views:42Ethnoveterinary medicine and livestock farm technologies represent a junction of innovation and tradition with the aim to accomplish livestock systems that are resilient, productive, and sustainable to meet 21st century challenges, especially the antimicrobial resistance issue. This article aims to emphasise the impacts of integrating ethnoveterinary medicine with livestock farm technologies on the performance of indigenous goats in South Africa. With the growing concern over antimicrobial resistance in livestock, there is a pressing need for complementary approaches to the health of animals and their productivity. In this review, the aim evaluated whether an integrative approach could offer a sustainable alternative that benefits animal health and productivity while addressing the concerns of antimicrobial resistance. Comparative study designs across multiple indigenous goat farms were used to integrate specific medicinal plants in ethnoveterinary medicine with present-day livestock technologies that are used to monitor the effects on livestock performance indicators and antimicrobial resistance patterns. In the treated populations, the outcomes were indicative of substantial improvements in reproduction and growth rates, and alongside, there has been a notable decrease in the markers of antimicrobial resistance. Therefore, it is proposed that through these discoveries, the integration of these traditional and modern approaches not only improve the performance of indigenous goats but also contribute immensely to the mitigation of risks associated with antimicrobial resistance.
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In vitro analysis of the effect of ragweed extract against Monilinia laxa
117-120Views:109Nowadays in Hungary nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia). According to the public opinion the ragweed is a weed. From agricultural and public health point of view it is exceptionally dangerous plant. As it contains a number of useful active ingredients, based on this the ragweed is consider a medicinal plant. Our goal was to present that the ragweed contains antifungal active substances as well. In the experiments we used the pre-flowering plants with roots and we extracted the biological active components of dried plant. We tested the biological activity of the extracts against Monilinia laxa in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Monilinia laxa.
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Andrological examination of Hungarian Large White and Landrace boars
5-10Views:217The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.
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Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:177The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group. -
The importance of millet production in regional production, with special emphasis on climate change
141-146Views:153Regional production is a traditional production structure developed adjusting to the geographical, climatic, biological and soil conditions in given production regions, a certain territorial specification of agricultural production, and a type of farming that best fits the natural conditions and takes the biological needs of plant and animal species into account as fully as possible. The most probable element of risk in plant production is the changeable, extreme weather. That is the reason why the specific characteristics of the place of production and the characteristics of regional production should be considered to a greater extent. The establishment of the range of varieties appropriate for the place of production is the key issue in regional production. One of our historically grown cereal plants that perfectly fits regional production is millet. Due to its short growing season, favourable reproduction ratio and the fact that it is relatively undemanding, it used to be grown in larger quantities in the middle ages. Its good nutritional values made it an important food item, but over time, as a result of industrialisation and technological progress; it has been eclipsed by other cereal crops. In our country it is mainly used to cook porridge, but it is also used in the form of flour and as a base material in the spirit drinks sector. In the recent decades, millet has been applied only in a small area, mostly as a secondary crop in areas that dried out from drainage water in late spring, or as a replacement of extinct sowings due to its late sowing time. Water will be the most significant factor for the future of agriculture, especially considering climate change.My examinations took place in the area of the Institutes for Agricultural Research and Educational Farm of University of Debrecen, in the Research Institute of Nyíregyháza, in a small-plot experiment with four replications in 2016. -
Occurrence and damage of the alfalfa root longhorn beetle (Plagionotus floralis Pallas, 1773) in old alfalfa stands in Hungary
82-88Views:101The alfalfa root longhorn beetle is a pest of small importance in old alfalfa stands. It is rarely cited in the international, national literature, or even mentioned in specialized manuels. Plagionotus floralis is common in Hungary but its population density is low. It is a polyphagous species but it has importance - regarding the references – only in alfalfa. This paper analyses data gained in old alfalfa fields at two different regions of the country (Máriabesnyő, Debrecen). Damage of P. floralis was 56% in the roots of an 8-year-old stand in Máriabesnyő. Lenghts of tunnels were between 3 and 22 cm. In spite of the tunnels the alfalfa plants were viable. There was no root longhorn beetle in the 15-year-old Debrecen stand, however it has been found former in the region. The reason for the important density of P. floralis in Máriabesnyő could be the considerable diversity and covering of flowering weeds around the field. Adults of P. floralis feed on flower pollen and nectar. As to the Debrecen area,weeds were cut regularly, so the edge vegetation was poor. Present conditions (no pest control on alfalfa fields, overuse of old and failing alfalfa stands, many untrained growers) favour the reproduction and possible damage of alfalfa root longhorn beetle.
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The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
135-140Views:161Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.