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The effect of bentonite on the quantity change of soil microorganisms, the CO2-production and the activity of saccharase enzyme
287-293Views:84We examined the impact of bentonite – the perspective improving material of sandy soils – and treatments of livestock manure composted with bentonite on sandy soils, within the framework of a small-plot experiment.
The adjustment of the experiment was made on the Experiment Site of the Nyíregyháza Research Centre of the University of Debrecen, Centre of Agricultural Sciences (UD CAS). We collected soil samples from parcels treated with increasing bentonite doses (5, 10, 15, 20 t/ha) on the one hand, and from the parcels treated with livestock manure composted with increasing doses of bentonite, on the other.
We performed laboratory research in the soil microbiology laboratory of the Soil Science Faculty of UD CAS DAS, during which we determined the total number of bacteria, the quantity of microscopic fungi, the number of cellulose-decomposing bacteria, the CO2-production of the soil and the activity of saccharase enzyme.
During the evaluation of the examinations, we made a statistical analysis using SPSS 9.0. We determined the average of measurements, the standard deviation of controls, the standard deviation, the significance value and we also performed a correlation analysis.
Concerning the impacts of bentonite treatment and the treatment of livestock manure composted with bentonite on the examined microbiological features of sandy soil, we can summarize the following:
• Our results prove that the microbiologic activity of the soil has increased owing to the impact of bentonite treatments regarding total number of bacteria and the quantity of microscopic fungi. ”Pure bentonite” treatments – although not significantly in every case – increased these values, but larger doses decreased them. The treatments of livestock manure composted with bentonite resulted in a larger increase regarding both parameters.
• The number of cellulose-decomposing bacteria was increased by the low doses of both series, and was decreased by the higher doses. Higher bentonite doses decreased it in a higher – significant – degree than those of treatments of livestock manure composted with bentonite, whose low dose caused salient number of bacteria.
• Regarding the carbon-dioxide formation, we have experienced an increase even in the case of low dose treatments (nevertheless, the increase did not prove to be significant), but – similarly to the quantitative changes in the number of cellulose-decomposing bacteria – the large doses of both series of treatment decreased the CO2-production of the soil.
• Bentonite also increased the activity of saccharase enzyme significantly. We learned that”pure bentonite” treatments increased the activity of the enzyme to a higher degree than composted treatments. Moreover, it can be stated that the treatments of larger doses of both bentonite and livestock manure composted with bentonite have decreased the enzyme activity – not significantly, though.
• Based on the correlation analysis, it can be stated that as an impact of the treatments, the microbiological activity of the soil has also increased with the increase of the number of soil microbes, as in both treatment series we have experienced a tight positive correlation (r=0.81-0.82) between the change of total number of bacteria and the CO2-production of soil. In both treatments, there was a medium correlation between the total number of fungi and soil respiration (r=0.63-0.63). Furthermore, it can be stated that the usage of organic manure had a positive effect on the activity of cellulose-decomposing bacteria, as in this treatment series there was a positive correlation (r=0.65) between this physiological group and CO2-production. Both treatments prosperously impacted the activity of saccharase enzyme, because there was a medium correlation (r=0.62-0.64) between the activity of the enzyme and soil respiration. -
Different soil fertility conditions depending on different land use methods
169-172Views:85In precision nutrient management the most important aspect is adaptation but we should consider the possibility of the long-term improvement of soil fertility within the less fertile landscape zones. This possibility can be evaluated principally by long-term field experiments, which are running on similar soil types. The results of these field experiments can indicate that which soil fertility status should be attained. Some more important soil fertility data, (such as pH, P-, K- and soil organic matter (SOM) content) of a long-term field experiment with increasing farmyard manure(FYM) doses or equivalent NPK fertilizers, set up on an Eutric cambisol, are presented. The yieldincreasing capacity of FYM doses was only 82%, as compared to the equivalent amount of mineral NPK, but long-term FYM treatments resulted in 10% higher SOM content than that of equivalent NPK
fertilizer doses. The studies indicate that SOM content is a function of local climate and clay content of the soil, and neither long-term high FYM doses can increase SOM content steadily above a supposed steady-state value. However we have to make efforts to keep the optimum level. The lowest soil reactions developed both with the highest NPK doses and without any fertilization. AL-P2O5 content of soil was increased more by mineral fertilization than by FYM treatments, but in case of AL-K2O content there was no difference between the fertilization variants. However the highest doses of both fertilization variants increased soil nutrient content to an excessive degree. Wecould get very valuable data from the unfertilized control plots as well, where long-term yield data suppose 48 kg ha-1 year-1 air-borne N-input. -
Effects of some herbicides on the microbiological characteristics of soil nitrogen cycle under maize plantation
93-100Views:77Nitrogen is a key element for the living organisms and influence not only for the quantity but for the quality of the yield, considerable. Availability of nitrogen from the soil is influenced by several microbiological processes of the Nitrogen-cycle. Among the intensive agricultural production the herbicide application cannot be omitted more information needs therefore about the inhibitor effect of herbicides on the different microorganisms.
An experiment was set up on calcareous chernozem soil under maize culture. Effect of four different herbicides (Acenit, Frontier, Merlin, and Wing) was investigated. The effect of herbicides was measured to four microbiological parameters of the Nitrogencycle (abundance of nitrifying bacteria, nitrate solubilisation, biomass nitrogen and urease enzyme activity). There were singledouble- and five times of recommended doses of herbicides applied for two onsecutive vegetation periods.
From the results of the different doses of herbicides, the following can be stated:
– The Acenit has a stimulating effect on nitrifying bacteria in general. The Frontier and Merlin also influenced the quantity of nitrifyers, however in certain cases decreased in another cases increased the number of bacteria.
– The double doses and five times doses of herbicides was found to be increasing the nitrate content of soil, -especially in 2006.
– The quantity of microbial biomass nitrogen increased in the 60% of treatments and decreased in the 40% of the treatments.
– Except of the result of Wing in 2006 and Merlin in 2005, the effect of simple dose herbicides was the smallest on the urease enzyme activity. According to the results the effect of Merlin was positive; the effect of Wing was negative on the soil enzyme’s activity.
Regarding the application of four different herbicides in three different doses on the microbiological parameters of soil (at two consecutive years-) in 39% of the treatments has resulted a significant inhibitory effect, 28% of the treatments, however have significant stimulating effect on the parameters measured. More than 50% of the inhibitory effect was measured in case of the Wing, at more than 50% of the Frontier the microbiological parameters have not changed. -
Nutrition reaction of different varieties of winter wheat (Triticum aestivum L.) bred in Karcag
63-67Views:146In our study the nutrition reaction of four varieties of winter wheat (KG Bendegúz, KG Kunhalom, KG Kunkapitány, KG Széphalom) has been investigated. In the experiment the effect of twenty different nutrition doses on the yield and thousand kernel weight of the wheat varieties has been studied. Significant difference could be figured out among the yield and thousand kernel weight of the wheat varieties, so the choice of the proper variety determines the quantity of yield. KG Kunkapitány had the highest yield, while KG Széphalom had the highest thousand kernel weight in the experiments.
Investigating the effect of the nutrients on the yield the conclusion was that all nitrogen doses had significant yield increasing effect compared to the untreated plots, but among the 40, 80 and 120 kg ha-1 doses there was no statistical difference. In the case of phosphorus the 40 kg ha-1 dose showed statistically verifiable increase of the yield, while potassium doses have no influence on the yields. The thousand kernel weights reached the maximum values where the highest nutrient doses were applied.
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Nutrient Supply Effects on the Fruit Quality of Apple (Malus domestica Borkh.)
230-235Views:103The aim of our two year study is to research the effect of nutrient supply on apple fruit quality, and to explore the relationships between selected fruit quality parameters.
Observations were made in Kálmánháza (in the eastern part of Hungary), on a commercial apple orchard. In this experiment, we studied the nutrient supply reaction of four apple cultivars (Golden Delicious, Granny Smith, Idared and Jonathan Csány) under different N and NPK doses. The following fruit quality parameters were studied: fruit diameter, fruit height, fruit weight, flesh firmness, colour-coverage and we studied the density of foliage.
The research results showed that N fertilization has a great effect on fruit quality. This is shown in the cases of increase of fruit size (fruit diameter, fruit height, fruit weight). The increase is proportional with the N doses, accordingly the highest positive difference was observed by using 100 kg/ha N doses. It is important to note that moderate N doses (75 kg/ha) plus P and K additions also had positive effects. There approached the values of 100 kg/ha N, and even exceeded its values in the cultivar Golden Reinders. The increased N doses enlarged the standard deviation, on the other hand, this parameter was low in the cases of balanced NPK fertilization. The nutrient supply increased the vegetative area (density of foliage) in addition to the generative parts, in particular only N fertilizer. However the denser foliage hindered the growth of fruit weight and colour-coverage, and also decreased the fruit quality and the flesh firmness of cultivars, which have a negative effect on storageability.
A linear correlation was demonstrated between the fruit weight and colour-coverage, or between fruit weight and flesh firmness. The character of their relationship was similar, but the direction differed: high fruit weight was with high colour-coverage, but with low flesh firmness. -
Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
109-115Views:150The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms were compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant. The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.
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Effect of different N doses on maize yield and quality
97-101Views:164The effect of N doses on the yield and nutritional values of the Sushi (FAO 340) maize hybrid were analysed in three years (2018, 2019, and 2020). The analyses were performed at the Látókép Experimental Station of the University of Debrecen on calcareous chernozem soil, in a striped, small-plot, non-irrigated long-term field experiment. In the experiment, in addition to the non-fertilized treatment (A0), the N-fertilizer doses were applied as basic fertilizer and top dressing. The 60 and 120 kg N ha-1 dose (A60, A120) applied as spring basic fertilizer were followed by two phases of top-dressing in V6 (V690, V6120) and V12 (V12150, V12180) phenophases; the amounts were +30 and +30 kg N ha-1.
Maize yields were affected to varying degrees by crop year. The highest yields in 2018 and 2020 were recorded in the same V6150 treatment, while in 2019 the highest yield was obtained in the A120 treatment.
Increasing the N doses resulted in an increase in the protein content of the maize kernel. Depending on the fertilizer treatments and the crop year effect, the protein content of maize kernels varied between 6.2–10.2 g x 100 g-1. In all three years, the protein content was the lowest in the control treatment (A0) and the highest in the V6150 treatment.
The starch content ranged from 70.7 to 77.9 g x 100 g-1 in the average of the three years. In 2020, it was significantly higher in all nutrient treatments than in the other examined two years. The highest starch content - except for 2020 (A120, 77.9 g x 100 g-1) - was recorded in the A0 treatment (74.2, 72.3 g x 100 g-1).
The oil content of maize kernels varied between the values of 3.8 and 5.2 g x 100 g-1 in the average of three years. In terms of oil content, the results for 2018 and 2019 can be considered the same, while in 2020 it was significantly lower. Fertilizer treatments did not significantly affect the oil content of maize in any of the years.
The fertilizer dose applied in the V12 phenological phase was not effective in terms of yield and nutritional content (protein, starch and oil content).
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Examination of the chlorophyll content of maize hybrids of different maturity groups at different N fertiliser doses
159-162Views:119Nitrogen fertilisation is a critical point of maize production. Five hybrids of different maturity dates were examined in a field experiment, three treatments (different application dates) and three basic fertiliser doses (0, 60, 120 kg ha-1 N) were used. At the 6-leaf-stage of maize, each fertilisation level of the 2nd and 3rd treatment was given 30 kg N ha-1 fertiliser active ingredient in addition to the basic fertiliser doses with the exception of the control plots and further 30 kg N ha-1 fertiliser was applied at the 12-leaf-stage. The final fertiliser doses were 0, 90 and 150 kg N ha-1 in the second treatment and 0, 120 and 180 kg N ha-1 in the 3rd treatment. The whole amount of the basic fertiliser (ammonium nitrate) was applied in the spring, one month before sowing.
The relative chlorophyll content of the maize leaves was measured, with a Minolta SPAD-502 measurement device. The measurements were carried out at the 6-leaf growth stage (V6) of maize on the youngest fully developed leaf of the 6th, 7th and 8th plants from the second row of each plot.
There were significant differences in the SPAD-readings measured at the V6 phenophase of maize between the hybrids (p<0.001) and the fertiliser treatments (p<0.05). The regression analysis did not show any correlation between the SPAD-values and fertilisation.
The highest significant SPAD-reading and yield were obtained by applying 120 kg ha-1 N. As a result of the regression analysis performed on yield, it can be concluded that the correlation between fertilisation and yield in the 1st and 2nd treatment was moderately close (r=0.439, r=0.480) and it was close in the 3rd treatment (r=0.513). The correlation between the SPAD-readings and yield was the closes in the 2nd treatment (r=0.639), while the SPAD-value had a 40.9% influence on yield (p<0.001).
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Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment
77-85Views:137The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the changes of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.
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The Effect of Sowing Time and Nutrient Supply on the Yield Stability of Maize
75-80Views:126Sowing time, nutrient supply and plant number play crucial roles in the yield stability of maize. The productivity of various hybrids, each with its own genetic characteristics, was tested for three different sowing times and five different fertilizer doses. The highest yields were achieved at the third sowing time (17. V.), which is unusual, because the second half of the summer was rainy and was favourable for late sowing. The seed moisture content at harvest was higher than the optimal 14-15% at the third sowing time, the hybrids, which have intensive bleeding dynamics, couldn’t reach the lower seed moisture content at harvest of the early sowing. In that case we have to decision whether the plus yield of the third sowing time cover the drying costs.
Some hybrids produced the highest yields by N 120, P2O5 75, K2O 90 kg/ha active agent but the higher fertilizer doses depress the yield. The other part of the hybrids were able to produce high yield by bigger fertilizer doses. On the whole the agro-ecological optimum of the NPK fertilization was N 120-160, P 25-100, K 90-120 kg/ha active agent, but the N 80, P2O5 50, K2O 60 kg/ha fertilizer doses was the most effective. -
Studies of the effects of N fertilizers and Microbion UNC biofertilizer on microelement content of horseradish (Armoracia macrocarpa)
41-45Views:117A field experiment on calcareous chernozem soil was performed to study the effects of different N and bacterial fertilizers on the nutrient content of horseradish (Armoracia macrocarpa). In the experiment the trials were arranged in a randomized block design with three replications, applying three levels of NH4NO3 and different N fertilizers, namely ammonium-nitrate, urea and calcium-nitrate, with or without application of Microbion UNC biofertilizer.
In the present paper the changes and distribution of manganese, zinc and copper contents of the horseradish plant are summarized by the
effect of different treatments.
The Mn content of leaves were higher in all cases than those of roots, but Zn mainly accumulated in the roots. The distribution of copper within the horseradish plant was more equalized than that of Zn and Mn. Different N fertilizers and increasing doses of ammonium-nitrate had effects mainly on the microelement contents of leaves. The highest Mn contents of plant were measured in treatments of Ca(NO3)2 and Ca(NO3)2+Microbion. The lowest ammonium nitrate dose (N1) decreased the Mn content of leaves compared to control, but further doses
(N2, N3) did not alter these values any longer. Microbion UNC biofertilizer did not have any effect on the Mn content of roots, but we measured higher Mn in leaves in some combined treatments. Ca(NO3)2 increased the zinc content in leaves and roots in a noticable manner. With the increasing of NH4NO3 doses, the Zn content of leaves and roots augmented significantly. Neither N fertilizers (or the increasing doses of NH4NO3) nor the biofertilizer application influenced the Cu content of horseradish plant.
N fertilizers had higher effects on the microelement content of horseradish, the biofertilizer’s effect was smaller and was not the same in every treatment. -
Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
253-265Views:288Agricultural management practices – directly or indirectly – influence soil properties.
Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization doses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).
The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.
With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds
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Productivity and Nutrient Reaction of Maize Hybrids
78-83Views:82Several factors influence the quantity and stability of maize yield, the most important being the nutrient supply, the hybrid and precipitation. In 2004, during the maize growing season the precipitation was more than the 30 year’s average, with 68.3 mm, but the distribution was unfavorable. The experiment was carried out in Debrecen at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization.
The favorable results reached were due to the rainy season. The average yield varied between 7.78-9.67 tha-1. The DK 440, PR37M34, PR38A24, PR39D81 and PR36R10 of the hybrids reacted to higher fertilizer doses with significant growth yields, the yield (more than 11 tha-1) was the highest for N200, P125, K150 fertilizing. The other hybrids, DKC 5211, Mv Vilma and MV Maraton, gave similar results at the N120+PK fertilizer dose and the ensuing doses depressed the yield. Fertilization was more effective thanks to the precipitation. The fourth and fifth dose increased or decreased maize yield depending on the nutrient reaction of the hybrid. The agro-ecological optimum of NPK fertilization was N120, P75, K90 kgha-1.
During the experiment we tested the moisture loss of five hybrids. The rainy crop year’s effect on the seed moisture content at harvest was higher than in previous years. The seed moisture content hybrids at harvest which have shorter crop years (FAO 300) was 18-19%, and hybrids with longer vegetation periods had more than 20% seed moisture content. DK 440 hybrid had the intensive moisture loss of the five hybrids, at the start of the measurement, the seed moisture content was higher than 40%, and it decreased to 18.6% by harvesting. -
Effect of the fertilization on some quality parameters of winter wheat
105-110Views:106We studied the different mineral fertilizer rates effect on the baking quality of five different winter wheat genotypes (GK Öthalom, Lupus, KG Széphalom, GK Békés, Mv Mazurka) on chernozom soil in 2008.
The bigger fertilizer doses increased the wet gluten content, gluten elasticity and the protein content. The high doses of fertilization has got a moderate effect on the valorigaph value, water absorption, and falling number. The optimum level of the fertilizer doses were the N90-120+PK.
The Pearson’s correlation analysis proved strong relationships between the fertilization and gluten content, the quality of the gluten, a valorigraph value, and the protein content. The analysis verified a strong correlation between genotype and falling number, and the protein content.
The variety Mv Mazurka has got the best baking quality parameters. -
N-fertilization using „Biofert” in Sustainable Maize Production
30-33Views:114In synthetic fermentation of lysine (amino-acid) a by-product (Biofert) originates which can be characterized by 6% N-content and other ingredients (vitamins, enzymes, micro-elements etc). In small and large plot experiments Biofert was studied in different agroecological (cropyear, soil), biological (genotypes) and agrotechnical (non-irrigated and irrigated; N-splitting etc) conditions in order to obtain information about agronomic efficiency and environmental effects of its applications.
Our results proved that Biofert has the same agronomic efficiency as traditional N-fertilizers (applied in equal doses and splitting), but Biofert has economic and environmental advantages (less N-leaching in soils) for maize production. We found a special interaction between N-supply and irrigation. In maize production (irrigation) with the optimum application of nutrient- (N-fertilization, Biofert) and water- supply we could stabilize maize yields at a high level (11.0-14.0 t/ha) fairly independently of agroecological factors. When applying Biofert in autumn, NO3-N leaching was less in 100-200 cm chernozem soil-layers than for applications of traditional N-fertilizer. There were no differences between different maize genotypes concerning the agronomic efficiency of Biofert. In maize production 120-190 kg/ha N (chernozem soil) and 165 kg/ha N (meadow soil) doses of Biofert were the optimum doses in splitting applications (autumn + spring). -
The effect of gamma irradiation on the germination and growth of Turda Star maize hybrid dry samples
23-25Views:64In order to study the effects of gamma rays on germination indices i.e. germination index (GI), total germination (GT) and the
germination energy (GE), of maize hybrid, Turda Star current experiment was conducted. Dry seeds of Turda Star maize hybrid were
irradiated with 2, 5, 15, 30 and 50 Gy, using a 60Co-gamma radiation source.
Results showed that gamma irradiation affected all the above mentioned parameters except germination percentage. Total
germination and germination energy was significantly affected at higher doses of gamma rays. However, increasing doses of gamma
rays did not have significant effects on seed germination percentage. The other growth parameters showed declining tendency with
increasing doses of gamma irradiation. -
Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:77Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
The impact of climatic factors on the relative chlorophyll content and yield of a maize hybrid in a long-term experiment
71-77Views:211The impact of the climatic factors of crop year on the relative chlorophyll content of maize was examined for three years. The examinations were carried out on the Látókép Experiment Site of the University of Debrecen on calcareous chernozem soil in a small-plot, non-irrigated long-term field experiment with strip plot design. In addition to a non-fertilised (control) treatment, nitrogen (N) fertiliser doses were applied as base and top dressing. The 60 and 120 kg N ha-1 base dressing doses were followed by two top dressing doses at the V6 and V12 phenophases.
Averaged over the different fertiliser treatments, SPAD readings increased in all three years as the growing season progressed. The highes SPAD value increase was observed in the average crop year (2017) at the V12 phenophase (11.8), which further increased at the R1 phenophas, by 3,7. No significant Spad value difference was observed between the average (2017) and the dry year (2018) at the V6 growth phase. However, in the wet crop year (2016), the V690 treatment provided the statistically highest relative chlorophyll content (46.8). At the V12 phenophase, the base dressing dose of 120 kg N ha-1+30 kg N ha-1 (V6150) showed to be successful in two years (2016 and 2018), while in 2017, the base dressing dose of A60 was successful. The impact of crop year on relative chlorophyll content can be clearly shown at the R1 growth stage. In all three years, the significantly highest relative chlorophyll content could be achieved at different nutrient levels: A60 in 2016, V6150 in 2017 and V690.
In a wet year (2016), higher yield could be achieved as a result of the 60 kg N ha-1 base dressing and 30 kg N ha-1 at the V6 growth stage (V690) as top dressing in comparison with 2017 and 2018, when higher fertiliser dose (120 kg N ha-1 base dressing and 30 kg N ha-1top dressing at the V6 growth stage) was needed to achieve a significant yield surplus.
Altogether, averaged over the different treatments, the highest yield (12.48 t ha-1) was observed in the wet year, when the relative chlorophyll content was also the highest (50.6).
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Az NPK-trágyázás hatása a kukorica tápelemfelvételének dinamikájára, öntözött és nem öntözött viszonyok között
23-27Views:168The effect of NPK-fertilization on the dynamics of nutrient uptake of maize (Zea mays L., cv. Clarica) was examined on chernozem soil under irrigated and non-irrigated conditions in a field experiment.
The following results were made:
• the element concentrations in the plant decreased over time,
• there is no difference between the dynamics of nutrients on irrigated and non-irrigated sites because rainfall was satisfactory for plants in vegetation period,
• the N doses not only significantly increase the nitrogen content in maize, but also have a noticable effect on Ca and Mg concentrations,
• because of the acidifying effect of N-fertilizers, increasing the amount of N-fertilizer increased the Mn, Zn, Cu content of the plants,
• the P doses have a significant effect on the maize P and N content and the Zn concentration of the plant via P-Zn antagonism in the soil,
• as the high K doses treatments alter the ion ratios in the soil, the Ca, Mg content of the plant decreased. -
The effect of sulphur and nitrogen supply on the growth and nutrient content of spring wheat (Triticum aestivum L.)
65-70Views:209Sulphur is an essential element for plants. Decreasing sulphur deposition from the air, and the use of more concentrated phosphate fertilizers, which contain no sulphur, has led to reports of sulphur deficiencies for wheat. Sulphur deficiency significantly affects yield and also the quality of wheat. The pot experiment was set up on calcareous chernozem soil at Látókép, Hungary, test plant was spring wheat (Triticum aestivum L). Seven treatments were used where nitrogen and sulphur were supplied as soil fertilizers in increasing rates (NS1, NS2, NS3) and in foliar fertilizer as well (NS1+fol., NS2+fol., NS3+fol.). Plant aboveground biomass production was determined in samples taken in the stages of development BBCH 29-30, 51-59, 61-69, 89. The nitrogen and sulphur content of straw and grain were measured. N/S ratios of grain and straw were calculated. The weights of grain were ranging between 8.6–16.1 g/pot. NS2 and NS2+fol. treatments produced the highest values. Foliar fertilizer had no further effect on grain. Analysing the values of the straw, it was observed that tendencies were similar to values of grain. The NS2 treatment produced the highest weight of straw and the NS3 rate already decreased that amount. The obtained results show the unfavourable effect of excessively high rate applied in NS3 treatment. The supplementary foliar fertilizer had no significant influence on the weight of straw. Both N and S-uptake of plant was very intensive at the stem elongation stage, then the N and S-content of plant continuously decreased in time in all treatments. The N-content of grain ranged between 2.215–2.838%.The N-content of grain slightly increased with increasing of nitrogen doses. In the higher doses (NS2, NS3) foliar fertilization slightly increased the nitrogen content of grain, although this effect was not statistically proved. The N-content of straw varied from 0.361 to 0.605%. The growing dose of soil fertilizer also considerably increased the nitrogen content of straw. Foliar fertilization further increased the nitrogen content of straw. The S-content of grain ranged between 0.174–0.266%. The lowest fertilizer dose (NS1) significantly increased the sulphur content of grain. The further increasing fertilizer doses (NS2, NS3) did not cause additional enhance in sulphur content of grain.The foliar fertilizer also did not change the sulphur value of plant. The increasing amount of soil fertilizer and the supplementary foliar fertilizer had no effect on the sulphur content of straw. The treatments influenced the N/S ratios of grain and straw. On the basis of experimental results it can be concluded that the examined nitrogen and sulphur containing soil fertilizer had positive effect on the growth and yield of spring wheat grown on the calcareous chernozem soil. The soil fertilizer application enhanced the grain nitrogen and sulphur content. The highest rate of fertilizer (600 kg ha-1) proved to have decreasing effect on the yield. The sulphur and nitrogen containing foliar fertilizer did not have significant effect on the yield parameters but slightly increased the nitrogen content of plant. -
Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
23-28Views:324A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.
On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.
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Evaluating of soil sulphur forms changes in long-term field experiments of Látókép
71-76Views:161The aim of this work was to evaluate the changes of different sulphur forms (soluble, adsorbed) in chernozem soil in a long-term field experiment supplied with increasing doses of NPK fertilizers for a long time. In addition, other objective of this study included the examination of the applicability of recommended extractants of the different sulphate fraction in Hungarian soils. A long-term field experiment was established at the Research Station of Látókép of the University of Debrecen in 1984. In addition to control, two levels of NPK fertilizer doses have been used with irrigated and non-irrigated variants. Winter wheat and corn were cropped in a crop rotation on plots. Soil samples were collected in three different development stages of winter wheat, at the stage of stem elongation (April), flowering (May) and ripening (June of 2018) from the topsoil (0–20 cm) of experiment plots. Water-soluble inorganic sulphate was extracted with 0.01M CaCl2 solutions. The soluble plus adsorbed sulphate was extracted with 0.016M KH2PO4 solution. Sulphate was measured by turbidimetric method. 0.01M CaCl2-SO42— ranged between 0.293–1.896 mg kg-1 and the 0.016 M KH2PO4-SO42- varied between 5.087–10.261 mg kg-1. The values of KH2PO4 SO42- was higher than that of CaCl2-SO42-, because KH2PO4 extracted the adsorbed and soluble fractions of sulphate, while CaCl2 extracted the soluble sulphate fraction. The amount of absorbed sulphate was calculated by the differences of KH2PO4- SO4 and CaCl2-SO4. The KH2PO4 characterizes mainly the adsorbed sulphate fraction much more than the water-soluble fraction. KCl is the most widely used extractant for the determination of plant available sulphate content of soil in Hungary; therefore, KCl-SO42- fraction also was determined. The KCl-SO42- ranged between 0.328–2.152 mg kg-1. The CaCl2-SO42- and KCl-SO42- fractions were compared and based on Pearson's linear correlation, moderate correlation was established (r=0.511) between them. In all three extractant (0.01M CaCl2, 1M KCl, 0.016 M KH2PO4) higher sulphate fractions were measured in the fertilized plots where superphosphate had been supplied for ages until 2010. The arylsulphatase activity of soil also was determined and ranged between 9.284 and 26.860 µg p-nitrophenol g-1 h-1. The lowest value was observed in the treatment with highest NPK2 dose, both in irrigated and non-irrigated areas.
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Changes in the Macro-, Mezo-, and Microelement Contents of Maize Hybrids in Relation to the Level of Nutrient Supply
126-130Views:66n my research, I measured the effect of NPK fertilisation on the macro- meso- and microelements content of maize hybrids in 2001. The experiment was set in the demonstration garden of the Department of Crop Production and Applied Ecology in the Agricultural Centre, at the University in Debrecen. The soil of the experiment is calcerous chernozem soil. Five fertilisation steps were applied. Besides the control the smallest rate was 40 kg N; 25 kg P2O5; 30 kg K2O of active ingredients. The largest rate was five times more than the smallest one: 200 kg N; 125 kg P2O5; 150 kg K2O, which is equal to 475 kg mixed active ingredients. The NPK treatment significantly influenced the macrolement content in several cases. The N content was the lowest in the control treatment. Compared to this the fertiliser treatments significantly increased the N content of hybrids. However the highest amounts of potassium and phosphorus could be measured in the control and the lowest amounts could be measured at the N 200+PK kg/ha treatment.
The Ca content of hybrids was the highest in the N 120+PK kg/ha treatment, while their Mg and Zn content was the highest in the control treatment. The lowest amounts were weighed in the N 200+PK kg/ha treatments, that in several cases resulted in statistically proved decreasement compared to the control or the lower fertilizer doses. Considering the two mesoelements and Zn the most favourable results were obtained in the case of the Norma SC and DK 366 SC hybrids.
Summing up what has been said moderate amounts of fertiliser doses (N 40-120+PK kg/ha) had a favourable influence on the micro- and macroelement content of hybrids. -
Growth and clorophyll content dynamics of winter wheat (Triticum aestivum L.) in different cropyear
101-105Views:108The experiments were carried out at the Látókép experimental station of the University of Debrecen on chernozem soil in a long term winter wheat experiment in the season of 2011 and 2012 in triculture (pea-wheat-maize) and biculture (wheat-maize) at three fertilisation levels (control, N50+P35K40, N150+P105K120). Two different cropyears were compared (2011 and 2012).
The research focused on the effects of forecrop and fertilisation on the Leaf Area Index, SPAD values and the amount of yield in two different cropyears. We wanted to find out how the examined parameters were affected by the cropyear and what the relationship was between these two parameters and the changes of the amount of yield.
Examining the effects of growing doses of fertilizers applied, results showed that yields increased significantly in both rotations until the N150+PK level in 2011 and 2012. By comparing the two years, results show that in 2011 there was a greater difference in yields between the rotations (7742 kg ha-1 at N150+PK in the biculture and 9830 kg ha-1 at N150+PK in the triculture). Though wheat yields following peas were greater in 2012, results equalized later on at N150+PK levels (8109–8203 kg ha-1).
Due to the favorable agrotechnical factors, the leaf and the effects of the treatments grown to a great extent in 2011, while in 2012 the differences between treatments were moderate. Until the N150+PK level, nitrogen fertilisation had a notable effect on the maximum amount of SPAD values (59.1 in the case of the biculture and 54.0 in the triculture). The highest SPAD values were measured at the end of May (during the time of flowering and grain filling) in the biculture. In the triculture, showed high SPAD values from the beginning. The same tendency could be observed in the 2012 cropyear, although increasing doses of fertilizers resulted in higher SPAD values until N150+PK level only from the second measurement. Maximum SPAD values were reached at the end of May in both crop rotation system
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Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:138The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations.