Comparision of growth of mature all-female and mixed-sex Common carp (Cyprinus carpio L.) stocks in RAS65-68Views:397
The common carp (Cyprinus carpio L.) is the most important fish species in Hungary, it is more than 70% of the total Hungarian fish production. The common carp production is important not only just in Hungary but in Middle-East Europe, as well as Southeast Asia. Majority of the production comes from fishpond culture. If the production sector wants to meet the increasing customer demands, there is need to intensify research on the intensive fish production opportunities for example all-female common carp technologies. The all-female technology is one of a genom-manipulation technology. Its production showed better growth rate than mixed-sex population in pond culture. Our experiment combined the recirculation aquaculture system (RAS) and the all-female common carp stocks intensification technologies. The reason for the experiment, is that there are no result about the growth of all-female common carp growth in RAS.
The experiment used the „Tatai grey scale type” common carp stocks. We propagated two all-female stocks (T2 and T3) and a control group (TK). Due to technological characteristics of RAS, the water quality parameters were the same for all treatments and corresponded to the technological tolerance of common carp.
The experimental period was from July 10, 2019 – November 20, 2019. Result of growth performance showed that the growth of mixed-sex stock was significantly higher than all-female stocks; (Control=3692.0±590.5g, T2=3438.8±415.4g, T3=3294.1±659.1g). Feed conversation ratio (Control=1.3±0.1 T2=1.5±0.2 T3=1.6±0.5) and SGR% (Control=0.8±0.0 T2=0.7±0.1 T3=0.7±0.1) were similar.
By the results it can be said the all-female common carp technology has neither advantages nor disadvantages compared to the mixed-sex stock. The all-female technology can be beneficial if the consumers need female common carps. It is worth continuing the experiment and examine how the stocks will perform above 3kg body weight.
Study of the Interaction Between Common Carp (Cyprinus carpio) and Silver Crucian Carp (Carassius auratus gibelio Bloch)9-12Views:97
Some of the main questions in the fish farming are the good manuring and the success of the feeding. For the best result is very important that only those fishes take place in the fishponds what we want. It seems to be easy but difficult to do. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the rearing season, weekly test fishings were made to control the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower growth rates and productivity in common carp. In the control pond (without silver crucian carp), the average weight of the common carp was 25 g and the yield/hectar was over 900 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 9 g and the yield/hectar was only 158 kg.
The effect of silver crucian carp (Carassius auratus gibelio Bloch) on the yields and the production value of common carp (Cyprius carpio) fingerlings5-10Views:78
During the last few years, the spread of the silver crucian carp has caused significant problems in pond fish culture. One of the main challenges of successful fish production in fishponds, is to rid ponds of the silver crucian carp. The nature of the damage caused by silver crucian carp is a complex question. To get into the ponds, the crucian carps occupy the territory before the common carp, and competes for food with common carp. The silver crucian carp has a less favorable food coefficient than the common carp, and it’s value is also lower. Therefore, the income of the fish farm decreases. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0,- 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. At the end of the experiment, the yield/hectar and the production values were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower yields and production values.
Study of the Interaction Between Common Carp (Cyprinus carpio L.) and Silver Crucian Carp (Carassius auratus gibelio B.)16-19Views:80
During the last few years, the spread of the silver crucian carp caused significant problems in pond fish culture. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the breeding season, weekly test fishings were made to examine the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower productivity and growth rates in common carp. In the control pond (with no silver crucian carp), the average weight of the common carp was over 20 g and the yield/hectar was over 700 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 4.5 g and the yield/hectar was only 123 kg.
Review on the fatty acid profile and free fatty acid of common carp (Cyprinus carpio)99-105Views:66
Carp or ponty in Hungarian, is considered commercial freshwater fish, which is an adaptable species in both wild and cultured conditions. Carp has high nutritional value content, favorable taste, it is rich in protein, and low in saturated fat. The nutritional content in fish is composed of many chemical constituents and influenced by many factors. One of the components that its content may be different due to internal and external factors is fatty acids, which may vary depending on endogenous and exogenous factors. The endogenous or internal factors include the genetic, size, sexual maturity, and life cycle phase. While microclimate, water quality, quality of food or diet habit, and the amount of available food or starvation are considered as exogenous or environmental factors. Freshwater fish has the ability to convert essential fatty acid into long chain polyunsaturated fatty acid like AA, EPA, and DHA. Most results showed that palmitic acid and oleic acid were the dominant SFA and MUFA in carp both for wild and farmed carp in all seasons. The PUFA for wild carp was mainly dominated by DHA, while on farmed carp by LA. It confirmed that high LA content in farmed carp was related to the diet habit. The amount of lipid and FA were changed in line with the season. Even the statistical analysis showed no significant difference, but some studies showed a contrasting result. Moreover, most obtained results acknowledged that FA tends to decrease during the spawning period. The amount and composition of FA were affected by the total lipid content. The lipid must be broken down into simpler compounds such as FA or FFA for the metabolism of fish. The result of metabolism then transported into the utilising tissue and used as energy.
Influence of 17-alpha methyl testosterone on the production parameters of common carp (Cyprinus carpio L.) fry37-43Views:219
During our research we aimed at finding an answer as to what extent the different concentrations of 17-alpha methyl testosterone incorporated in the diet of common carp fries can influence the production parameters of the species, as well as how efficient their sexreversal can be with the use of this method. To this end, an aquarium experiment was conducted in the course of which four different hormone treatments were set and monitored. The fish feed was enriched with 17-alpha methyl testosterone in 50 ppm, 75 ppm, 100 ppm, 500 ppm dosages.
The obtained figures revealed that the hormone treatments had no influence on the production parameters and conservation of the common carp fries. Further on, our team is to determine the sex of the fish through the examination of gonads during autopsy when they reach the 500 g average weight.
Furthermore, a male specific test method which was supposed to be of great help in our attempt to select the sex-reversed specimens in the subsequent processes was also put to the trial. During the experiment the DNA-isolation of different sample types (muscle tissue, fin, mucus) of common carp with identified sex was successfully carried out. The extracted PCR product was examined with agarose gel. Our results indicated that the ccmf2 marker was applicable, however, the obtained figures were not reliable.
The effect of dissolved oxygen on common carp (Cyprinus carpio) and basil (Ocimum basilicum) in the aquaponics system89-96Views:238
Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum basilicum).
Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.
In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments.
Effect of different weaning time on the growth and survival ofCommon carp (Cyprinus carpio L.) larvae55-59Views:268
Within our experiment, we studied the combined effect of live food application and feed training on the production parameters of carp larvae. In addition, we examined whether there is a difference in the growth rate and survival rate of the specimens of Szeged mirror landrace reared in similar stocking density but with different treatments.
Specimens of Szeged mirror landrace from induced propagation were placed into 12 pieces of 40-liter aquaria. For the 4 different treatments, (K, A3, A6, A9), live food (Artemia salina) was fed, as well as feed training was applied.
We also sought to find an answer as to how our feeding and rearing conditions can affect the production parameters of common carp fries and what impact these conditions may have on their survival rates. At the end of the 2-week experiment we determined the individual weight gain of the carp fries, their survival rate and the biomass growth. The purpose of the study is to examine the optimum timing of shifting from live food to feed for carp larvae.
Examination of resistance against Aeromonas hydrophila infection on genetically different common carp variants (preliminary results)7-11Views:74
Septicemia disease caused by the bacterium Aeromonas hydrophila is a major problem for fish farmers around the world. In our experiment resistance to the disease was investigated on carp variants with diferent genetical backgrounds. 96 different genetic variants(„families”) were created by crossing various individuals of 4 carp strains (2 wild and 2 cultured ones). Families were reared until reaching the size of 10 gramms and 100 fish of each families were marked with an individual electronic identifier. Marked fish were divided into 5 groups. Fish from one group were infected with A. hydrophila and mortality were registered during 2 weeks. Based on mortality data, the most resistant and most susceptible families will be selected using population genetics methods.
The effects of bioactive feed additives for the production parameters of common carp (Cyprinus carpio) fingerlings in intensive recirculating system35-41Views:208
For the appropriate quantity and quality of fish meat the pond farmers need to use up to date nutrition and feeding technologies. Recently the intensification of the carp production is stepping up with the availability the proper quality of the artificial feeds. The using of different feed additives make a better feed conservation ratio, faster growing rates and more uniform stocks.
In our experiment what is made with common carp (duration of the experiment was 8 weeks long) we compared two different kinds of feed additives in different doses. One of them contains only natural mineral elements and the other additive is a natural origin probiotics. Both additives were used in the quantity of 0.5, 1 and 2% of the total feed. By the result of the 8 weeks experiment it could be said, that the using of the humic acid based mineral elements feed additive. Makes a better FCR comparing with the control stock, but was not as good as the using of the probiotics. At the humic acid treatments the more doses, the better FCR value, but at the probiotics it wasn’t true. The 0.5% probiotic treatment gave the best result. It is concluded that the using of the probiotic feed additives always gave a better performance of the fishes (average weight, biomass, FCR) than the humic acid treatments. The using of the feed additives hasn’t got an influence for the survival rate of the carps.
Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)49-56Views:240
In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to improve immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.
Effect of fish feeds with high vegetable oil and low fish oil content on the growth performance and innate immune system of common carp (preliminary results)7-12Views:75
Fish meal and fish oil are very important components of fish feeds used by aquaculture. They contain poly-unsaturated fatty acids, which are essential nutrients both for fish and human nutrition. However, sources of fish meal and fish oil are limited, there is a need for new feed components to supply the increasing fish production and the increasing demand for fish feed. The aim of AQUAMAX project, supported by the European Union is to replace the fish meal and fish oil with vegetable oils. As a participant of this project, group of immunology in Research
Institute for Fisheries, Aquaculture and Irrigation (HAKI) examined the effect of three different fish feeds on the growth performance, innate immune response and resistance against Aeromonas hydrophila infection of common carp in an in vivo experiment. Fish feed supplemented with Camelina oil, but still containing fish oil significantly increased growth performance of experimental fish and their resistance against the infection. Fish feed containing linseed oil only did not enhance the growth performance, and it had a negative effect on resistance against infection. Based on the experimental results it can be concluded that fish oil used in fish feeds cannot be replaced effectively with vegetable oils, but can be supplemented with them.
Stress Response of four Common Carp (Cyprinus carpio L.) Varieties with Different Genetic Background26-29Views:67
Four different carp varieties were tested for stress reaction, with the help of 10 °C cooling and 100 g/l crowding. The selected varieties represented a broad genetic spectrum of carp species. The stress was monitored by measuring plasma cortisol, glucose and lysozim levels. In each test different varieties showed significantly different reaction to stress. This means that they differ in the trait of general resistance. Thus, this trait can be used in selection programmes and the acquired information helps to understand fish-environment interaction and specific resistance. These traits should be used in selection to improve the breeding of new more tolerant species for intensive farming or for organic production.
Comparison of the technological background of aquaponic systems47-52Views:241
Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.
The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yield and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NO3¯N were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).
Practical experiences of a designing and operating a pilot aquaponic system27-32Views:232
Aquaponics is the combination of fish farming (aquaculture) and the soilless cultivation of plants (hydroponics). The aquaponics system is an artificial, recirculating ecosystem, in which bacterial processes convert the waste materials in the water used for fish rearing into plant nutrients, and therefore with the generated heat it is suitable for culturing economically valuable plants, and thus it mitigates the nutrient laden and quantity of the intensive fish producing systems’ effluent water.
The primary goal of our 12 separate unit’s aquaponics system was to gain experience. We would like to find the right plant species, which are fit for that medium, and their crop can be sold. Besides the plants, our attention focused on the fish. Two fish species were included in the experiments, the common carp and barramundi. It was difficult to create them a perfect living space, besides a constantly changing conditions temperature. Apart the above mentioned we had a problem with the number of individuals per tank, the deformity of the fish body and the too high volume of pH (we registered continuous values above 8.4). We get by carps 4.7 grams of weight gain during 15 weeks, because of the bad conditions.
The main problems at the plants are caused by aphesis and protection against sunburn. Even so we have got the multiples of field yields for each plant species. At salad has grown twice of field yields, tomatoes one and half, kohlrabi than 3.5 times more. The causes of multiple yields are the continuous balanced water and the nutrient uptake of plants. Each plant species fit for cultivating in aquaponics and their crops are delicious, chemical -free, safe and marketable. The plants should be more concentrated. After the experiment, it has been determinated that the carp is suitable for aquaponics, but greater weight gain could be achieved with optimal selection of size of rearing units.
Preliminary results of the combined production of duckweed Spirodela polyrhiza and common carp (Cyprinus carpio) in an aquaponic system83-89Views:60
As a result of population growth, increasin amount of food is needed, and agriculture is under an increasing burden to meet these needs. Traditional agriculture is often attacked due to its environmental impact. We must find alternative, environmentally friendly ways to produce more food. Aquaponics is a closed system in which we can produce both fish and plants at the same time. Duckweed species are small, aquatic, floating plants belonging to angiosperms. It can potentially be an alternative protein source, due to its high protein content, good amino acid supply, and rapid growth. Under suitable conditions, it doubles its weight in 2–4 days, and can reach a yield of 30 t ha-1 year-1in dry matter. It forms a carpet on top of the water and can be found in slow-moving or still waters. Since they are resistant to a wide range of nutrient concentrations, they are also suitable for cleaning wastewater (such as eutrophicated lakes, sewage reservoirs, liquid manure storage). Fish feed is the primary nutrient source for aquaponic systems, which usually contains fishmeal. If duckweed can be used as an alternative for fishmeal in the feed, it could improve the sustainability of the aquaponic and aquaculture systems. In this study, the aim was to develop an optimal harvesting protocol for duckweed Spirodela polyrhiza under aquaponic conditions. In a four weeks experiments, four harvesting protocols were set up, a control where only biomass measurements were made, a 25% group where 25% of the biomass at the time of measurement was harvested, and a 50% and a 75% group where at the time of measurement 50% and 75% of the biomass was harvested. Three replicates were used per treatment. We weighed the biomass every week and removed the amount corresponding to the group. Based on the preliminary results, it can be said that more biomass was obtained in the groups with the 25% harvesting protocol and the control group.