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  • The impact of different fertilization methods on some microbiological soil characteristics

    In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
    experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
    Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
    Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated. 
    In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
    • Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
    • The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
    • The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
    • The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
    • On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
    • In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
    resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly. 
    • The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
    • On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
    • The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
    • Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
    Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer.

  • Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil

    In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collected four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
    the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
    Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
    − The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
    − The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
    − On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment. 
    − The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition. 
    − Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources. 
    Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
    the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity. 
    Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
    mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility.


  • Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil

    A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
    nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
    (116 kg ha-1 N) in combination with Microbion UNC bacterial fertilizer (2 kg ha-1) were applied as treatments in a randomized
    complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
    was chernozem with medium sufficiency level of N and P and poor level of K.
    Our main results:
    The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
    --N and NH4
    +-N and also the quantity of soluble organic-N were
    almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
    --N increased to the greatest
    extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
    - -N and total-N contents in the plots
    treated with ammonium-nitrate, the largest NH4
    +-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
    treated with urea.
    Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
    the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
    --N, organic-N and total-N compared to the
    values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
    content of soil in more cases were higher than that of values with artificial fertilizer treatment.
    As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
    UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
    yielded a decrease in these values compared to the control.
    All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
    fertilizer increased AL-Mg values in any cases.

  • Comparative examination of a bacterium preparation (BACTOFIL® A10) and an artificial fertilizer [CA(NO3)2] on calcareous chernozem soil

    In a small-pot experiment a bacterium preparation, Bactofil® A10 and an artificial fertilizer containing Ca(NO3)2 in different dosages were studied on calcareous chernozem soil, concerning the readily available nutrient content of soil (nitrate-nitrogen, AL-phosphorus, ALpotassium content of soil, some soil microbial characteristics (total number of bacteria and fungi, cellulose-decomposing and nitrifying bacteria, CO2-production of soil), and the amount of the plant biomass.
    The readily available nutrient content of the calcareous chernozem soil increased due to the treatments, except for the change in the soil nitrate-nitrogen content, which did not measure up to the control due to the effect of high-dosage Bactofil.
    The treatments also influenced the examined microbial characteristics of the soil positively. The artificial treatments significantly increased the total number of bacteria and the number of cellulose-decomposing and nitrifying bacteria. The low-dosage Bactofil significantly increased the number of cellulose-decomposing bacteria and both Bactofil dosage significantly increased the number of nitrifying bacteria. The measure of the soil respiration grew in all treatments, but significantly only in Ca(NO3)2 fertiliser treatments.
    The quantity of the plant biomass was grew in a low-dosage Bactofil and significantly in the artificial fertiliser treatments. The highest plant biomass quantity was measured in the high-dosage artificial fertiliser treatment.
    In the correlation analyses we found some medium positive correlation between the soil chemical, microbiological parameters examined, and the plant biomass in the case of both treatment-forms. 
    Based on our results Ca(NO3)2 artificial fertiliser treatments on calcareous chernozem soil proved to be more stimulating regarding the
    examined soil characteristics and the amount of the plant biomass, but the low-dosage Bactofil also positively influenced the majority of the
    soil characteristics examined in terms of nutrient supply.

  • Effects of Cropping Year and Artificial Fertilization on Alveographic Quality of GK Öthalom Winter Wheat Variety

    Qualification of winter wheat became more complex for Hungary, after the expansion of the EU. Use of Chopin alveograph is a general method in Western- and Southern Europe. This method is not traditional in Hungary, so determination of alveographic properties of Hungarian winter wheat varieties is very important. Artificial fertilization is one very important component of applied agricultural engineering, which effects winter wheat quality. The another factor is the weather or rather the cropping year, which effects winter wheat quality too. We examined both of them and the alveographic quality of GK Öthalom winter wheat variety between 1996 and 2003.
    We found that cropping year has significant effect on the alveographic quality of GK Öthalom winter wheat variety on each treatment of artificial fertilization. We found no connection between quantities of precipitations of vegetation periods and the W values of GK Öthalom.
    We found a strong connection in 1999 and 2003 as well as a very strong connection in 1996, 1997 and 2002, between the increase of fertilizer dose and W values of GK Öthalom. Treatments of artificial fertilization had a significant effect to W values of GK Öthalom in 2001 and 2002. The differences between the two methods are because of large standard deviation of the results.

  • Alternatives of sewage sludge use in the crop production

    The produced plants reduce the greenhouse effect because they fix CO2 that contributes to the causing of the greenhouse effect with about 50%. The production of fertilizers is not only a costly process but it needs a considerable energy at the same time. Nowadays, the reduction of the proportion of the fertilizer is significant. One of the reasons of this is that during the production such by-products are produced in a big quantity in which the necessary vegetal nutrients can be found in a considerable measure these enrich the organic matter of soil. The latter is essential condition for the microorganisms in the soil, without which the sustainable plant cultivation can not be achieved. Besides high prices of artificial fertilizers the utilization of the wastes is economically justified. Finally the other reason for the reduction of a usage of artificial fertilizer is that the wrong use of the fertilizer may cause environmental pollutions. I examined the cultivation application of the sewage sludge in laboratory circumstances during my work.

  • The effect of biopreparations in pot experiment

    In pot experiment the effect of Amykor and Organic Green Gold bioproducts and their combinations with NPK fertilizer on some soil properties (chemical parameters) and on the biomass of testplant were studied. The experiment was set up in 2012 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. The studied soil typein the pot experiment was humus sandy soil from Debrecen-Pallag with onion (Allium cepa) test plant. At the end of the experiment (after 4 week) in our laboratory the samples of soil and plant were determined. The nitrate-nitrogen, AL-soluble phosphorus and potassium content of soil, the weight of green onion leaves, the wet weight of bulb and root of onion and biomass of onion. The results of the study were the following: – The treatments influenced positively the nitrate-nitrogen, the AL-soluble phosphorus and potassium content of soil. – The most effective treatments were the artificial fertilization (NO3-N) and the NPK+ simple dose of Amykor (AL-P2O5 and Al-K2O). – The NPK fertilization and the NPK+OGG (sprinkle in every 10 days) combinations had significant positive effect on the weight of green onion leaves. – The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally. – The OGG treatment (sprinkle in every 10 days) had significant effective impact on the wet weight of bulb and root of onion. – The biomass of onion was increased by the artificial fertilization and OGG (sprinkle in every 10 days) treatment.

  • Technological and Technical Issues of Efficient Nutrient Supply

    Efficient plant production requires modern production technologies, including a nutrient supply of a scientific basis. The harmonic nutrient supply of quality products that are required by market demands can be produced by the optimal combination of a compound system of requirements. These requirements are partly preparatory (soil investigations, yield measurements, preparation of digital field maps) and are partly based on the knowledge of the most significant characteristics of the applied nutrients. Most artificial fertilizers are applied by spinning disc fertilizer spreaders all over the world, therefore, it is advisable to use them in Hungary as well. Importantly, these machines should be well adjustable, capable of the exact measurement and control of the spread quantity, as well as of even spreading. My report has investigated only the most significant technical requirements affecting the even application of nutrients.

  • Nitrogen Content of Hungarian Soils and Nitrogen Fertilization

    For crop production and agricultural production, the most important natural resource is the soil that can optionally renew. Paralelly with this, soil plays a major role in the geological and biological cycle of elements. As a result of the big (geological) and small (biological) element cycles, the elements and combines neccessary for organisms can accumulate in the soil creating suitable living conditions for plants and other organisms. Soil is a heterogenous system both horizontally and vertically, and soil constituents show great variety in all the three dimensions, in addition, most of the parameters can also change between two examination dates. When talking about the factors influencing plant production, one should take into account this variation and heterogenity in time and space. When making fertilization recommendations, these factors should all be considered. In any consultation system, most of the mistakes and errors made are due to the unsatisfying soil testing and the negligence of soil heterogenity. In the practice of fertilization the biggest mistake is the improper soil sampling, then comes the methodical mistake of soil testing, which is followed by the inaccuracy of instrumental analysis and the subjectivity of result evaluation, but the latter two are negligible compared to the others. Under normal, i.e. production conditions, the quantity and distribution of nutrients in the soil are greatly dependent upon the applied technology, the amount and form of the applied natural and artificial fertilizers and the quality of fertilization.
    Fertilization recommendations are needed because in the layer which is accessible for plant roots only a part of the nutrient content is available for plants in a specific production cycle. An illustration of this is that though the upper 1 m layer of an average chernozem soil contains more than 5000 kg N, 12000 kg K2O and 1500 kg P2O5 (form of expression mostly used in Hungary), the application of fertilizer doses which are just fractions of these quantities is essential. This is due to the fact that the available amount of the total nutrient content depends from the quality of soil, the environmental factors (the physical and chemical qualities of the soil) and the specific nutrient’s qualities (solubility, adsorption). Knowledge of these processes and the examination of the factors influencing the actual nutrient content are vital for working out a fertilization practice, which does not put more strain on the environment than neccessary.

    All of the above mentioned should be considered when applying inputs in the fields. In a well-functioning practice that considers the economic and environmental conditions (unfortunately the present production and economic conditions do not enable an appropriate level and degree), three nutrients are supplemented generally (and were supplemented in the last decades): nitrogen, phosphorus, potassium.
    Studying the nutrient balance of the Hungarian field production’s last hundred years, we can draw some interesting conclusions.
    The nutrient balance became positive for nitrogen and potassium in the second half of the 1960’s, while for phosphorus it was positive from the first half of the 1960’s and this period lasted until the end of the 1980’s.
    Neither before the 1960’s, nor since the 1990’s has the amount of nutrients supplemented in a specific year reached the amount of the nutrient uptake of the same year.

  • Effect of agrotechnical factors on the yield of the Grolim asparagus (Asparagus officinalis L.) hybrid on acidic sandy soil

    Research of blanched asparagus has begun at the University of Debrecen Institutes for Agricultural Research and Educational Farm Research Institutes of Nyíregyháza in 2011. Establishment of the plantation took place in May 2011. The Grolim hybrid was used in the trial, 16 medium plot trial area has been formed under field conditions, with four repetitions and 36 m2 plot size. In the course of our studies, the effect of different nutriment supply methods (untreated, manure, sheep manure compost, fertilizer) has been analysed on the spear yield of the Grolim asparagus hybrid between 2013 and 2017.

    In our studies, the beginning of vegetative growth has been recorded upon the constant presence of 10 °C of average soil temperature in the case of the Grolim asparagus hybrid. The beginning and length of spear harvesting are both influenced by the time and dynamics of initial development in spring. During the analysed period, the dates of spear harvesting were various, the earliest being on 23rd March, 2014 and the latest on 23rd April, 2015; the rest of the three years have been varied within this one month interval. The total of heat units required for the vegetative development of spears has been determined; it provides important information for cultivation practice.

    Spear yields turned out to be hectic during the analysed period. In 2013 and 2014, yields have surpassed the amount of 50 kg/harvest period/plot in the case of every treatment version. However, in 2015 a significantly lower specific yield has been recorded due to the unfavourable weather conditions in spring; a yield decline of nearly 50% was recorded in the case of the control treatment compared to the previous years. Yield was also lower in the rest of the fertilization treatments compared to 2014; however, in these cases, the degree of yield decrease was around 5–10%, which suggests the yield stabilising effect of fertilization. In 2016, a slight yield increase was measured in comparison with the base year. In 2017, there was a decline of yield in the control treatment; however, the different fertilization treatments resulted in yield increase as compared to previous years.

    On the basis of our studies, it is clear that the best yield results have been provided by the artificial fertilization treatment in all of the five analysed years. It was followed by the sheep manure compost and manure treatments in terms of their effect on spear yield. During the three harvesting periods, the lowest yield on acidic sandy soil was recorded in the case of the control treatment. The most remarkable effect of nutriment treatments has been realised in terms of the decreased deviation of yield results, which perfectly represents the yield stabilising effect of nutriment supply in the case of perennial crops – asparagus – as well, even on a poor nutriment supply characteristic sandy soil.

  • Effect of cultivation factors on the yield and yield security of maize

    Cultivation factors have a significant effect on the yield and yield security of maize. Ensuring a suitable green crop is important. Tricultural crop rotation (pea–wheat–maize) in the average of 25 years provided a 2 t ha-1 higher yield compared to monocultural cultivation. A harmonious NPK nutriment supply determines yield and yield security, which can be especially realized by means of the application of precision cultivation technologies. Under average circumstances N 80 kg ha-1, P2O5 50 kg ha-1, K20 60 kg ha-1 active ingredient is the agro-ecological dosage of artificial fertilizer.
    Plant density is a factor that determines yield. Optimal plant density – beside the genetic characteristics of the hybrid – is mostly influenced by the level of water and nutriment supply.