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Population genetic results of Hungarian Botrytis cinerea isolates establishing new technologies with decreased chemical usage against grey mould
259-261Views:267Botrytis cinerea causes gray mold on a high number of crop plants. Information about the populations of plant pathogen fungi may help to develop new strategies for the effective and economic crop protection with reduced fungicide usage. Hungarian B. cinerea populations were characterized with using different molecular genetic parameters. B. cinerea group I strains, characterized with high rate of fenhexamid resistance, could be detected only in restricted number. The Hungarian B. cinerea populations were characterized with high genetic diversity, and the regular occurrence of sexual reproduction. These results highlight the importance of rotating different type of fungicide in the plant protection technology against grey mould.
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New Possibilities of Brown Bullhead (Ictalurus nebulosus) Farming
55-57Views:194The brown bullhead (Ictalurus nebulosus) has been settled in Europe at the end of Century XIX from North America. In Hungary it has been brought in 1902. The naturalization of this species was successful because the brown bullhead found adequate conditions for its life and reproduction in Hungary. But it was unsuccessful because lost its excellent growth.
In inland water habitats-and that’s why also fishponds-extraordinarily multiplied, but because of its slight growth this species is undesirable for the native farmers. The brown bullhead is concurrent for domestic fish species, on the other hand its weight not more than 150-200 g. Occasionally it was exterminated, but multiplied again and again.
In 1999 we placed out black bullhead offspring in a pool of a new intensive fish farm, in order to breed at least 300-350 g weight for the customers. The accidental trial was surprisingly successful. Consequently, the black bullhead prefers the artificial food, and produces excellent growth parameters and specific feed-live weight conversation ratio.
On the 20th of September 1999 there was placed 23 300 pieces of brown bullhead offspring, with 28 g average weight and 625 kg gross weight. When we sold them on the 22nd of February 2000, the gross weight was 3387 and the live weight gain was 2735 kg. The gross weight of the feed was 3041,8 kg, and the feed-live weight conversation ratio was 1,11 kg/kg.
Because of this successful trial the managers of the farm wanted to start systematic experiments to work out the details of the intensive farming of brown bullhead. We undertook to help to find the right place of the species in the Hungarian fish processing because of the increasing native and foreign demand. -
Occurrence and damage of the alfalfa root longhorn beetle (Plagionotus floralis Pallas, 1773) in old alfalfa stands in Hungary
82-88Views:300The alfalfa root longhorn beetle is a pest of small importance in old alfalfa stands. It is rarely cited in the international, national literature, or even mentioned in specialized manuels. Plagionotus floralis is common in Hungary but its population density is low. It is a polyphagous species but it has importance - regarding the references – only in alfalfa. This paper analyses data gained in old alfalfa fields at two different regions of the country (Máriabesnyő, Debrecen). Damage of P. floralis was 56% in the roots of an 8-year-old stand in Máriabesnyő. Lenghts of tunnels were between 3 and 22 cm. In spite of the tunnels the alfalfa plants were viable. There was no root longhorn beetle in the 15-year-old Debrecen stand, however it has been found former in the region. The reason for the important density of P. floralis in Máriabesnyő could be the considerable diversity and covering of flowering weeds around the field. Adults of P. floralis feed on flower pollen and nectar. As to the Debrecen area,weeds were cut regularly, so the edge vegetation was poor. Present conditions (no pest control on alfalfa fields, overuse of old and failing alfalfa stands, many untrained growers) favour the reproduction and possible damage of alfalfa root longhorn beetle.
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The importance of millet production in regional production, with special emphasis on climate change
141-146Views:416Regional production is a traditional production structure developed adjusting to the geographical, climatic, biological and soil conditions in given production regions, a certain territorial specification of agricultural production, and a type of farming that best fits the natural conditions and takes the biological needs of plant and animal species into account as fully as possible. The most probable element of risk in plant production is the changeable, extreme weather. That is the reason why the specific characteristics of the place of production and the characteristics of regional production should be considered to a greater extent. The establishment of the range of varieties appropriate for the place of production is the key issue in regional production. One of our historically grown cereal plants that perfectly fits regional production is millet. Due to its short growing season, favourable reproduction ratio and the fact that it is relatively undemanding, it used to be grown in larger quantities in the middle ages. Its good nutritional values made it an important food item, but over time, as a result of industrialisation and technological progress; it has been eclipsed by other cereal crops. In our country it is mainly used to cook porridge, but it is also used in the form of flour and as a base material in the spirit drinks sector. In the recent decades, millet has been applied only in a small area, mostly as a secondary crop in areas that dried out from drainage water in late spring, or as a replacement of extinct sowings due to its late sowing time. Water will be the most significant factor for the future of agriculture, especially considering climate change.My examinations took place in the area of the Institutes for Agricultural Research and Educational Farm of University of Debrecen, in the Research Institute of Nyíregyháza, in a small-plot experiment with four replications in 2016. -
Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:626The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group. -
Evaluation of the relationship between main type traits and longevity in Hungarian Simmental cows
205-209Views:425The Hungarian Simmental cattle is a dual-purpose breed, having good milk and meat production characteristics. Simmental cows have some other important traits, for example, a longer productive life. The longevity or productive life is the time period between first calving and culling. The conformation contributes to productive life, reproduction, and milk production.
The aims of this study were to analyze the longevity of Hungarian Simmental dual-purpose cows, to evaluate the effects of the size of the herd, age at first calving, main type traits (frame, musculature, feet and legs, mammary system), and combination of main type traits (frame and musculature, feet and legs and mammary system).
Animal, age at first calving, herd*calving, musculature, the mammary system as well as the combination of mammary system and feet and legs were significant effects on longevity. The highest risk ratio was observed for cows first calved after 31 months. The risk of culling increased with increasing scores of musculature and decreasing scores of the mammary system. The highest risk ratio was estimated in category 11 (lower scores of mammary system with lower scores of feet and legs). In this case, the risk ratio was 36% higher than the reference group.
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Andrological examination of Hungarian Large White and Landrace boars
5-10Views:378The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.
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Understanding leptin’s influence on calf development: A review of metabolic and reproductive mechanisms
145-153Views:618Leptin, a hormone primarily produced by adipose tissue, regulates the energy balance, metabolism, and reproductive functions. This review explores leptin's role in calf development from embryogenesis to puberty. Leptin expression is strongly influenced by the nutritional status, with circulating levels dependent on adipose tissue mass and modulated by metabolic hormones such as insulin and glucocorticoids. It plays a regulatory role in the embryonic cell division, adipogenesis, and postnatal energy metabolism. Additionally, leptin modulates appetite, the growth hormone secretion, and the reproductive system maturation. Its presence in the colostrum and milk highlights its significance in the early calf development, influencing the neonatal metabolism and immune function. As calves approach puberty, leptin serves as a key metabolic signal for reproductive maturation. A deeper understanding of leptin’s multifunctional role in calf development could contribute to improved livestock growth and reproductive management strategies.
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In vitro analysis of the effect of ragweed extract against Monilinia laxa
117-120Views:200Nowadays in Hungary nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia). According to the public opinion the ragweed is a weed. From agricultural and public health point of view it is exceptionally dangerous plant. As it contains a number of useful active ingredients, based on this the ragweed is consider a medicinal plant. Our goal was to present that the ragweed contains antifungal active substances as well. In the experiments we used the pre-flowering plants with roots and we extracted the biological active components of dried plant. We tested the biological activity of the extracts against Monilinia laxa in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Monilinia laxa.
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Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
25-29Views:296In the last 20-30 years, lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimizing lameness. Those areas are related to poor facilities, lack of straw, problems with labor and basic management.
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The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
135-140Views:353Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.
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Effects of water deficit on the growth and yield formation of maize (Zea mays L.)
143-148Views:336Maize (Zea mays L.) is the most important consuming cereal crop in the world after rice and wheat. This requires an understanding of various management practices as well as conditions that affect maize crop performance. Water deficit stress during crop production is one of the most serious threats to crop production in most parts of the world and drought stress or water deficit is an inevitable and recurring feature of global agriculture and it is against this background that field study of crops response to water deficit is very important to crop producer and researchers to maximize yield and improve crop production in this era of unpredicted climatic changes the world over.
A pot experiment was carried out to determine the effects of water deficit on growth and yield formation of maize. Two maize cultivars were used Xundan20 and Zhongdan5485. Three levels of soil water content were used in two stages of water control levels at two stages of the maize plant development
1. The JOINTING STAGE: A. CONTROL (CK) soil water content: from 70% to 80% of soil water holding capacity at the field, soil water content: from 55% to 65% of soil water holding capacity at the field, soil water content: from 40% to 50% of the Soil water holding capacity at the field.
2. The BIG FLARE PERIOD: A. CONTROL (CK) soil water content: from 75% to 85% of soil water holding capacity at the field, soil water content: from 58% to 68% of soil water holding capacity at the field, soil water content: from 45% to 55% of the soil water holding capacity at the field.
This research mainly studied the effects of water deficit on physiological, morphology and the agronomical characteristics of the maize plant at the different water stress levels.
The importance of these results in this experiment will enable plant producers to focus and have a fair idea as to which stage of the maize plant’s development that much attention must be given to in terms of water supply. -
Clay-pit systems fishfaunistic research in the Middle-Tisza
81-92Views:166After the regulation of the Tisza River the chance of successful fish propagation lessened. Natural spooning places in the river almost completely disappeared. The fish have to find an adequate place for their propagation in the flood plain. The period of spooning usually coincides with flooding of the river. At this time fish try to find the flooded shallow places for spooning. These parts mostly include the clay-pits beside dams, which were accidentally established during construction of the dams. At this place the fry can find the necessary food. After decreasing the flood the fingerlings and a part of the spooners are trapped inclay-pits as these latter ones are not connected with the river bed. The clay-pits usually desiccate during the summer. The trapped fish population is eaten by water birds or harvested by the local man population. This means a great loss for reproduction of some fishspecies.
Within the framework of the Regional Rehabilitation Program at Nagykörű, supported by the “WWF Hungary” and a “SAPARD project,” these clay-pits became connected with each other in a stretch of 5 km, and they were jointed to the Tisza by a collecting channel. The water level has been regulated by a flood gate so that the water enters the holes during the flood and is released later on in to the water bed.
Data on fish were collected from the Nagykörű Whole System, from the Anyita pond and some isolated wholes in Szandaszöllős in 2004 and 2005. First of all fish fry and fingerling were collected and the success of spooning at these places was examined. Fry was harvested by a 60 x 80 cm sized lifting net of 2x3 mm mesh size. In other cases nets used by anglers for catching prey fishes was also used. Data were also collected from local fishermen who participated in saving the fry and fishing of Anyita pond.
Of the several thousand caught fish specimens 28 species were identified, and among them 5 protected and 5 economically important species was found. Protected fishes were as follow: gudgeon (Gobio gobio), bitterling (Rhodeus sericeus amarus), weather fish (Misgurnus fossilis), spined loach (Cobitis elongatoides) and tubenosed goby (Proterorhinus marmoratus). Among economically important fishessamples of asp (Aspius aspius), carp (Cyprinus carpio), wels (Silurus glanis), pike (Esox lucius) and pikeperch (Sander lucioperca) were -
Cannibalism and its importance in Aquaculture: A review
43-54Views:1060Intracohort cannibalism may lead to significant losses during the early life stages of farmed fish. The mechanisms driving cannibalism dynamics are common across many fish species and are closely linked to the factors that influence growth variation within a population. This genetically encoded behaviour allows the individual to eliminate competitors in the competition for resources and increases the chances of survival and reproduction. Many fish species display aggression towards individuals of the same species, which can often lead to cannibalistic behavior. This foraging strategy is also present in intensive aquaculture systems, and its economical, ecological and phylogenetic implications have been debated by many researchers. Many fish species can be produced economically in recirculation aquaculture (RAS) systems, but the efficiency of larval and juvenile rearing remains a bottleneck for most species. The success of initial rearing depends on many factors. In addition to genetic factors, the level of cannibalism is mainly influenced by the applied production and feeding technology. Literature suggests that mortality during larval rearing can be as high as 50%, largely due to aggression. In the case of predatory fish species, the presence or absence of certain husbandry conditions can lead to even higher levels of cannibalism, which significantly reduces production yields by causing mortality and secondary injuries, thus further decreasing the survival rate. This review therefore aims to identify and quantify the effects of biotic and abiotic factors that influence cannibalism.
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Usability of vegetable extracts in the protection against Alternaria alternata
113-116Views:244In our country, wormwood ragweed (Ambrosia artemisiifolia) may cause serious problems. Nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia), which is believed useless weed. Allergological point of view, most problematic weeds adventive. However, many physiologically very beneficial compound also included, those with the effects have been known also by the Indians. On this basis, herbs can be thought of as ragweed. Our goal was to present that the ragweed contains antifungal active substances as well. In this paper we tested the biological activity of the extracts against Alternaria alternata F.00750 in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Alternaria alternata. The minimum effective concentration was 300 mg extract in a Petri dish, which was three days inhibited the growth of fungus. Full fungicidal effect was observed over dose 525 mg.
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Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:282The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals.