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Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary
193-200Views:116The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.
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Application of semen evaluation techniques
5-11Views:232Laboratory methods of semen evaluation are used to select males for artificial insemination. The current review describes several techniques that have been recently used for sperm analysis. Conventional microscopic methods in combination with the objective computerassisted sperm motility and morphology analyzers and flow cytometry, allows to obtain more precise information about the membrane and functional status of spermatozoa. By using several methods to detect motility, viability, acrosomal and capacitation status besides DNA integrity sperm biology and some of the mechanism involved in sperm cry injury can be better understood. The number of possible targets related to sperm quality is increasing, and possible that some of them could enable sperm analysis for predicting freezability and fertility to be improved.
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Evaluation of Origin and Conformation of Excellent Cows with Long Productive Lives
13-17Views:107In the last few decades a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Beside this reproduction biology of the population was affected adversely by the keeping-, and feeding technology. Serviceable lifetime has shortened and service period has lenghtened. Whereas profitable milk production needs cows with longer productive life, larger life-time production. In our work we analysed the productivity-, and reputation parameters of the 200 highest lifetime-productivity cows of Hungary. We found that inheritance has not got important role in the serviceable lifetime. Adaptability and good constitution of Holstein-friesian is proved by the fact that cows with large life-time production are reared in large plants, some of them in large stocks, industrial environment. We found during the analysis of reputation parameters that these prominent cows mostly have deeper body, higher dairy form, higher rear udder, smaller foot angle. We found some weak connection between the milk production and the reputation. Loose correlations between useful material content and some linear traits were found.
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Investigation of the heat shock gene Hsp70 polymorphism in different sheep genotypes
41-45Views:184Nowadays the climate change has an increasing effect on the animals. The warming climate brings up several problems on the area of the animal husbandry, which ones are really important. From the first time the living beings have defensive mechanisms against the heat shock. In current examination we use– from Hungary and from other countries collected –samples of sheep breeds, which are living on different climate. Our fundamental assumption was, that the animals living on other climate adapted to the changes of the environment and there are differeces in their genetic background. These fixed mutations we are looking for in the HSP70 heat shock gene, but we haven’t found any polymorphism yet. We are going to involve further breeds and more individuals in the investigations.
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The milk production and prolification of Saanen goats of various body condition and ages
49-52Views:110In our experiments we tried to find correlation between the age and the body condition of milking goats. We found that these factors significantly incluence the economic parameters of the goats; they have effect on their milk production and reproduction as well. Body condition is the lowest in case of the 3-5-year-old goats, whereas it is the highest in case of the 1-2-year-old. The highest number of kids (2.5) are produced by the 3-5-year-old, while the 1-2-year-old have the fewest kids. Lactation milk production is the highest in case of the 4-5-year-old animals, the lowest in the 1-2-year-old. The lactation period is the longest in case of the 3-4-year old animals in contrast to the younger. The difference is significant in each experiment.
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Impacts of ethnoveterinary medicine integrated with livestock farm technologies on the performance and antimicrobial resistance of indigenous goats in South Africa –A review
93-103Views:122Ethnoveterinary medicine and livestock farm technologies represent a junction of innovation and tradition with the aim to accomplish livestock systems that are resilient, productive, and sustainable to meet 21st century challenges, especially the antimicrobial resistance issue. This article aims to emphasise the impacts of integrating ethnoveterinary medicine with livestock farm technologies on the performance of indigenous goats in South Africa. With the growing concern over antimicrobial resistance in livestock, there is a pressing need for complementary approaches to the health of animals and their productivity. In this review, the aim evaluated whether an integrative approach could offer a sustainable alternative that benefits animal health and productivity while addressing the concerns of antimicrobial resistance. Comparative study designs across multiple indigenous goat farms were used to integrate specific medicinal plants in ethnoveterinary medicine with present-day livestock technologies that are used to monitor the effects on livestock performance indicators and antimicrobial resistance patterns. In the treated populations, the outcomes were indicative of substantial improvements in reproduction and growth rates, and alongside, there has been a notable decrease in the markers of antimicrobial resistance. Therefore, it is proposed that through these discoveries, the integration of these traditional and modern approaches not only improve the performance of indigenous goats but also contribute immensely to the mitigation of risks associated with antimicrobial resistance.
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Fractionation of chicken egg proteome by isoelectric point in liquid phase
39-42Views:205The application of proteomics is relevant to physiology, reproduction, immunology, muscle and lactational biology in animal science, altough its use is still limited. One of the greatest challenges of proteome analysis is the reproducible fractionation of the complex protein mixtures. The fractionation methods can increase the probability of biomarker protein discovery. The fractionation by liquid-phase isoelectric focusing is one of the prefractionation methods. As a result, protein fractions can be easily collected, pooled and refractionated. There is a lack in the knowledge of gel-based proteomic methods of egg as only a limited number of protocols can be found in the literature, thus sample purification and fractionation require a time consuming optimisation procedure. The aim of this study was to fractionate egg yolk and white proteins by isoelectric point in liquid phase.
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Comparison of Reproductive Performance of the Roe Deer (Capreolus capreolus, L.) Among Different Regions
41-46Views:96The potential and actual number of offspring of roe deer and the difference between these figures (prenatal and postnatal loss) significantly vary in each population yearly. The objective of this study is to examine the potential and actual number of offspring, the number of losses, and to find a link between the most important biological characteristics of does (body weight – BW, condition – KFI) and the number of raised offspring on four territories on the Great Hungarian Plain.
Where the number of corpora lutea (CL) is the highest, there the losses are the highest as well, and the number of raised offspring is the lowest (region I.). Here, the rearing loss is double that of the weakest territory (region IV.). Rearing losses can be associated with the fenotype of does (BW, KFI) but environmental factors also have determinative importance. Where the number of twin-calving does was the highest, I found four times more does without a fawn than where the number of twin-calving does was the lowest. The nursing success was the best (the losses were lowest) in the region where the potential offspring (number of CL) was also the lowest, but the coverage of the habitat and the proportion of forests were largest. The food supply for the animals in autumn and winter are not enough, the structure of the habitat has to be improved as well, so that it might become adequate for game protection in extreme weather conditions.
The results have to be considered as preliminary ones. It is essential to continue and extend the research to increase the reliability of the results. -
Population genetic results of Hungarian Botrytis cinerea isolates establishing new technologies with decreased chemical usage against grey mould
259-261Views:127Botrytis cinerea causes gray mold on a high number of crop plants. Information about the populations of plant pathogen fungi may help to develop new strategies for the effective and economic crop protection with reduced fungicide usage. Hungarian B. cinerea populations were characterized with using different molecular genetic parameters. B. cinerea group I strains, characterized with high rate of fenhexamid resistance, could be detected only in restricted number. The Hungarian B. cinerea populations were characterized with high genetic diversity, and the regular occurrence of sexual reproduction. These results highlight the importance of rotating different type of fungicide in the plant protection technology against grey mould.
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New Possibilities of Brown Bullhead (Ictalurus nebulosus) Farming
55-57Views:102The brown bullhead (Ictalurus nebulosus) has been settled in Europe at the end of Century XIX from North America. In Hungary it has been brought in 1902. The naturalization of this species was successful because the brown bullhead found adequate conditions for its life and reproduction in Hungary. But it was unsuccessful because lost its excellent growth.
In inland water habitats-and that’s why also fishponds-extraordinarily multiplied, but because of its slight growth this species is undesirable for the native farmers. The brown bullhead is concurrent for domestic fish species, on the other hand its weight not more than 150-200 g. Occasionally it was exterminated, but multiplied again and again.
In 1999 we placed out black bullhead offspring in a pool of a new intensive fish farm, in order to breed at least 300-350 g weight for the customers. The accidental trial was surprisingly successful. Consequently, the black bullhead prefers the artificial food, and produces excellent growth parameters and specific feed-live weight conversation ratio.
On the 20th of September 1999 there was placed 23 300 pieces of brown bullhead offspring, with 28 g average weight and 625 kg gross weight. When we sold them on the 22nd of February 2000, the gross weight was 3387 and the live weight gain was 2735 kg. The gross weight of the feed was 3041,8 kg, and the feed-live weight conversation ratio was 1,11 kg/kg.
Because of this successful trial the managers of the farm wanted to start systematic experiments to work out the details of the intensive farming of brown bullhead. We undertook to help to find the right place of the species in the Hungarian fish processing because of the increasing native and foreign demand. -
Occurrence and damage of the alfalfa root longhorn beetle (Plagionotus floralis Pallas, 1773) in old alfalfa stands in Hungary
82-88Views:116The alfalfa root longhorn beetle is a pest of small importance in old alfalfa stands. It is rarely cited in the international, national literature, or even mentioned in specialized manuels. Plagionotus floralis is common in Hungary but its population density is low. It is a polyphagous species but it has importance - regarding the references – only in alfalfa. This paper analyses data gained in old alfalfa fields at two different regions of the country (Máriabesnyő, Debrecen). Damage of P. floralis was 56% in the roots of an 8-year-old stand in Máriabesnyő. Lenghts of tunnels were between 3 and 22 cm. In spite of the tunnels the alfalfa plants were viable. There was no root longhorn beetle in the 15-year-old Debrecen stand, however it has been found former in the region. The reason for the important density of P. floralis in Máriabesnyő could be the considerable diversity and covering of flowering weeds around the field. Adults of P. floralis feed on flower pollen and nectar. As to the Debrecen area,weeds were cut regularly, so the edge vegetation was poor. Present conditions (no pest control on alfalfa fields, overuse of old and failing alfalfa stands, many untrained growers) favour the reproduction and possible damage of alfalfa root longhorn beetle.
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The importance of millet production in regional production, with special emphasis on climate change
141-146Views:185Regional production is a traditional production structure developed adjusting to the geographical, climatic, biological and soil conditions in given production regions, a certain territorial specification of agricultural production, and a type of farming that best fits the natural conditions and takes the biological needs of plant and animal species into account as fully as possible. The most probable element of risk in plant production is the changeable, extreme weather. That is the reason why the specific characteristics of the place of production and the characteristics of regional production should be considered to a greater extent. The establishment of the range of varieties appropriate for the place of production is the key issue in regional production. One of our historically grown cereal plants that perfectly fits regional production is millet. Due to its short growing season, favourable reproduction ratio and the fact that it is relatively undemanding, it used to be grown in larger quantities in the middle ages. Its good nutritional values made it an important food item, but over time, as a result of industrialisation and technological progress; it has been eclipsed by other cereal crops. In our country it is mainly used to cook porridge, but it is also used in the form of flour and as a base material in the spirit drinks sector. In the recent decades, millet has been applied only in a small area, mostly as a secondary crop in areas that dried out from drainage water in late spring, or as a replacement of extinct sowings due to its late sowing time. Water will be the most significant factor for the future of agriculture, especially considering climate change.My examinations took place in the area of the Institutes for Agricultural Research and Educational Farm of University of Debrecen, in the Research Institute of Nyíregyháza, in a small-plot experiment with four replications in 2016. -
Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:210The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.