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Investigation of the β-casein (CSN2) gene polymorphism in goat breed – Review
69-73Views:1551Milk plays an important role in the human nutrition as an important protein and energy source. Β-casein is the most abundant casein fraction in the goat milk. The β-casein gene has at least 9 polymorphism in goat breed (A, A1, B, C, C1, D, E, 0 and 0’). The most important alleles are the zero (0) and zero’ (0’) alleles, because they decrease the quantity of β-casein protein in milk. The lower quantity of the β-casein changes the properties of the coagulation and the allergenin milk. This review is about the β-casein gene polymorphism in goat breed.
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The variation of breast milk composition during the lactation stage for mothers from the Csík region
93-98Views:298The aim of our research was the examination of breast-milk composition from mothers living in the Csík region and to follow their milk composition variations during lactation. In this article we present the results obtained from three mothers, paying particular attention to essential components. The breast milk samples were collected from healthy mothers with similar habits and age. The milk samples were collected with a hand pump at the
same time after the feeding. The sampling period was from day 5 to the 14th weeks of lactation. The nutrition of mothers was recorded on a questionnaire, completed by the mothers themselves. Comparing our experimental results with data in the literature it was concluded that the milk protein content was very similar to the milk of mothers from other European countries, and is decreased during lactation.
The total saturated fatty acid content was lower, but the palmitic acid content was slightly higher. Regarding the essential fatty acid composition the linoleic- and the arachidonic acid contents were appropriate from a nutritional point of view. The linolenic acid was present in lower amounts, but the docosahexanoic acid was almost undetectable.
The iron and manganese contents were in good agreemen with published results, but the zinc content of the breast milk was lower and its copper content was higher. These differences in milk composition can be explained by the different nutritional habits of our subjects. -
Variations in major quality parameters of forage and medium quality winter wheat varieties in storage
249-254Views:424We analysed five parameters (moisture-, protein content, Hagberg’s falling number, wet gluten content and alveographic W (10-4 J) values) and the microbiological changes of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) during storage, to determine the tendency, type and volume of the of the change of this five qualitative parameters during storage.
We found that the examined winter wheat varieties retained their moisture, protein content and their Hagberg’s falling number, they did not change during storage.
A slight growth could be experienced in the values of wet gluten content for all the four winter wheat varieties in terms of the duration of storage (129 days). This result proved the theory of after-ripening, when gluten percentage improves qualitatively and quantitatively as well. The value of the quantitative growth was about 10% for all the four winter wheat varieties.
We placed a special emphasis on measuring the alveographic W (10-4 J) values during storage. All the four winter wheat varieties showed decreasing values of about 20-40%.
Microbiological examinations on the four winter wheat varieties showed that mould, mould flora and total germ count remained balanced with some slight variations and they did not change in terms of time under optimal storage conditions. -
Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:374The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations. -
Development of maize production technology that increase the efficiency of bioethanol production
17-26Views:402Maize is one of the most important crops worldwide and also in Hungary, it can be utilized for multiple purposes: as a feedingstuff, for human nutrition and for industrial processing. In the last decades, the per ha yield of maize varied greatly in Hungary, between 2004 and 2006, it was 6.82-7.56 t/ha, while in 2007, it was only 3.6 t/ha. Resulting from this, the price of maize became 2-2.5 times higher. The high price hinders bioethanol production. The largest per ton amount of bioethanol, 387 l, can be produced from maize.
In addition to its classical utilization as feed and food, the industrial use (especially for bioethanol production) of maize is increasin.
For industrial production, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2007.
The applied fertilization treatment was N 120, P2O5 80 uniformly, and five different dosages of potassium: K2O 0, K2O 100 (KCl), K2O 100 (Kornkáli), K2O 200 (KCl), K2O 200 (Kornkáli) kg/ha active ingredient. The applied plant densities were 40, 50, 60, 70, 80, 90 thousand plants/ha.
The yield of maize hybrids in the fertilization experiment ranged between 10.53 – 14.62 t/ha. Both regarding the form and dosage, 100 kg/ha Kornkáli proved to be the best potassium treatment. Regarding the inner content parameters, the highest starch content in the average of treatments was obtained for the hybrid PR36K67: 73.57%, and its yield was also the highest, so this hybrid proved to be the most suitable for bioethanol production. The highest protein content was observed for the hybrids KWS 353 (12.13%), which can be favourable for feeding purposes.
Most of the hybrids gave the highest yield at 80 thousand plants/ha plant density, however, hybrids PR36K67 and Mv Tarján achieved the highest yield at 90 thousand plants/ha.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
Study of some cooking and eating quality characters on some Egyptian rice genotypes
77-82Views:294Some Egyptian rice genotypes [i.e. Japonica (Sakha 104), Japonica/Indica (Egyptian hybrid1) and Indica (Giza 182)] were investigated to evaluate the cooking and eating quality characters. High significant differences in grain shape were observed among rice genotypes. Hulling, milling and head rice percentage were higher in Sakha 104 than other rice genotypes, while Indica type (Giza 182) recorded the lowest values in milling and physical characters. No significant differences were found in chemical composition of the three genotypes of rice was recorded, but Giza 182 had the highest protein content. All Egyptian rice genotypes were low in gelatinization temperature and soft in gel consistency. Japonica and Indica rice varieties were low in amylase content, while Japonica/Indica rice variety was intermediate. The use of RVA is considered a good index for palatability evaluation for milled rice flour and starch. The Indica and Japonica/Indica types are low in breakdown viscosity, but higher in cooked pasta than Japonica type. Japonica type recorded the best score in panel test, followed by Indica type, while Indica/Japonica rice variety was the least accepted by Egyptian consumer.
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The effects of drought stress on soybean (Glycine max (L.) Merr.) growth, physiology and quality – Review
19-24Views:430Abiotic stresses are one of the most limiting factors inhibit plant's growth, leading to a serious production loss. Drought stress is one of the most destructive abiotic stresses and is still increasing year after year resulting in serious yield losses in many regions of the world,
consequently, affecting world’s food security for the increasing world population. Soybean is an important grain legume. It is one of the five major crops in the world, an essential source of oil, protein, macronutrients and minerals, and it is known as the main source of plant oil and protein. Harvested area of soybean is increasing globally year after year. However, soybean is the highest drought stress sensitive crop, the water deficit influences the physiology, production and seed composition of this crop. We introduce a review for literatures concerning the changes of the above traits of soybean exposed to drought stress, with past explanations for these changes. -
Impact of chronic heat stress on digestibility of nutrients and performance of meat type ducks
51-55Views:371The aim of the study is to determine the effect of vitamin and mineral supplement under permanently high environmental temperature (30±1 °C) on the digestibility of nutrients, performance and furthermore the composition of duck meat in the growing period. A total of four hundred mixed sex 14 days old Cherry Valley type hybrid ducks were used for the study. Two experimental diets were formulated in the study (control and vitamin E, C and zinc supplemented diet). Based on the results the following conclusions were drown: the antioxidant defence system plays an important role in the reduction of heat stress generated lipid peroxidation process. Feed additives which have direct or indirect antioxidant effects can reduce the negative effects of heat stress on the ducks performance and meat composition. Digestibility of nutrients (Dry Matter, Crude Protein, Crude Fat) was not affected by antioxidant supplementation under chronic heat stress (30±1 °C). The performance was affected significantly by Vitamin C and E and zinc supplementation under heat stress (P<0.05). In the treated group the daily weight gain (dWG) increased and the feed conversion ratio (FCR) was improved significantly (P<0.05). The energy and protein conversion was decreased also significantly (P<0.05).
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Influence of nitrogen fertilisation on the technological quality of wheat
47-55Views:368Wheat is one of the most grown crops around the world. Its primary use is in the production of bread, bakery, and confectionery. The provision of essential nutrients, mainly nitrogen, plays a pivotal role in the growth and development of wheat. The wheat varieties used in the experiment are Alfold and Mv. Menrot. The rates of nitrogen used in the experiment are: 0, 200, 400, 600, 800, and 1000 kg N ha-1. The aim of this study is to evaluate the effect of nitrogen fertilisation on technological quality of wheat and to find the appropriate fertiliser rate to reduce pollution. The results indicate that nitrogen fertilisation did not show a significant effect on thousand kernel weight and test weight. However, nitrogen fertilisation significantly affected protein content, gluten content, Zeleny sedimentation index, and the falling number. The higher the nitrogen fertilisation the better the technological quality parameters of the wheat. The protein content, gluten content, Zeleny sedimentation index and the falling number were the lowest at 0 kg N ha-1 followed by 200 kg N ha-1 then 400 kg N ha-1 and the highest at 600 kg N ha-1.
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The composition of gluten proteins and their effect on the rheological properties of gluten
124-129Views:516Wheat is the major cereal component of bread in the world and is grown worldwide. Of the cereals only the bread wheats – and less the triticale – includes storage proteins that play an important role in the performance of gluten. Proteins of gluten complex may be present in two classes:
− low molecular weight (gliadin-) components, and
− high molecular weight (glutenin-) components.
Gliadins shown appreciable heterogenity and can be separated into 40-50 components with gel electrophoresis. The composition of gliadins is employable for the identification the wheat varieties and to investigate the varieties. In the decreasing electrophoretic mobility sequence may be distinguish α-, β-, γ- and ω-gliadins. A glutenin subunits may be include in two classes:
− high molecular weight glutenin subunits (HMW-GS),
− low molecular weight glutenin subunits (LMW-GS).
Wheat varieties can be identified by glutenin and their quality selection is also possible. The gliadin’s polypeptides encoding genes are located on the short arm of chromosomes 1A, 1B and 1D, 6A, 6B and 6D. Genetic coding for HMW subunits is located on the long arms of chromosomes 1A, 1B and 1D, the LMW-GS are also located on chromosomes 1A, 1B and 1D (Glu-3 loci) near the gliadin-coding loci.
Storage proteins affect the rheological properties of gluten by two factors:
1. The quality and quantity of the protein components of the gluten complex,
2. The interactions between the protein fractions. -
Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:396The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. -
Overview of the evolutionary history and the role in citric acid production of alternative oxidase
83-88Views:294All organisms are exposed to countless environmental effects, which influence in a disadvantageous way their life processes. They continuously adapt to the changing conditions and respond to the stress impacts by defence mechanisms. Through different signal transduction pathways they are able to increase or decrease the expression of their genes and consequently modify their metabolic processes. My interest focuses on alternative oxidase (AOX) enzyme whose expression is often increased under biotic and abiotic stress. The so far proven and putative functions of the AOX play a role in the ability of organisms to adapt to different conditions, such as heavy metals accumulation, pathogenic infection, oxidative stress and lack of oxygen or nutrients.
AOX is a member of the di-iron carboxylate protein family. Members of the di-iron carboxylate protein family are present in all kingdoms of life. They are considered to have ancient origin. It is believed that their sulfide-resistant and oxygen-reducing ability played a role in the survival of organisms during the transition between the anaerobic and the aerobic world. It is assumed that the AOX arose in eukaryotes through a primary endosymbiotic event, and this event made possible the development of mitochondria. Afterwords, vertical inheritance, and secondary and tertiary endosimbiotic events led to its spread among eukaryotes. It is assumed that bacteria obtained AOX by horizontal gene transfer from plants.
AOX-catalyzed alternative respiration plays an important role in the operation of energy-producing and biosynthesizing system of microorganisms. In these cases, the regeneration of reduced cofactors is an essential condition, and therefore may be rate-limiting for biotechnological processes, including the citric acid production.
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Trends in Dry Pea (Pisum sativum L.) Production
53-58Views:333Dry pea is an important, cool-season grain legume, which is grown worldwide on over 6 million hectares. The major producing countries outside Europe are China and Canada, followed by India, Australia, and the United States. France, Canada and Australia produce over 2 million hectares and are major exporters of peas. During the 1980’s, in developed countries of the European Union, pea production rose yearly by 6-10%, which represents a significant increase in both area and yield. Europe accounts for 50-75% of world pea production. In the 1990’s, the European Union produced 4-5 million tonnes of dry pea, of which 3-4 million tonnes were used for feed and 1 million tonnes for export. At the end of the 20th century, the growth in production was low, mainly because of the absence of support measures, and the better returns offered by other crops. In the countries of the former Soviet Union, dry pea was primarily used as feed and pea production dropped, due to a trend in livestock raising.
Food consumption of dry pea is concentrated in developing countries, where grain legumes represent a useful complement to cereal-based diets as a relatively inexpensive source of high quality protein. As a result, human consumption of grain legumes fell from 2,2 kg/capita in 1961 to 0,5 kg/capita in 1999. The importance of grain legumes in food protein supply decreased, while that of cereal products increased. Shortage of grain legumes has adverse effects on the nutritional standard of poor people in developing countries.
World dry pea production reached 16,7 million tonnes in 1990, with 3,7 million tonnes used as food, 11,4 million tonnes used as feed, and 1,0 million tonnes used as seed. Dry pea production was 10,9 million tonnes in 1999, and 3,5, 5,8 and 0,8 million tonnes was used as food, feed and seed, respectively. In the coming decades, world grain legume production and utilization as feed are expected to expand at a slower rate than in the 1980’s. Most of the increase is expected to occur in Eastern European countries, Canada and Australia, where production is anticipated to grow at 2% annually. The projection for the new millennium was derived from adjusted trends in area and yield over the period 1961-2000, based on FAO statistical data. -
The effect of compost made of sheep manure on the first cut of a semi-natural grassland
25-29Views:400In an experiment, two types of compost were tested on natural grassland in order to improve the productivity of a natural sward on solonetz soil. Both composts were made of on-farm produced sheep manure, but the second one was enriched in phosphorus. These fertilizers/composts are officially authorized and can be applied in organic farming. Zero application and three rates of fertilizer were tested (10 t ha-1, 20 t ha-1, 30 t ha-1) on 30 m2 experimental plots wit four replications. Dry matter, crude protein and net energy content for maintenance were measured and determined and their yields per unit area were calculated. We found that all the treated plots produced significantly higher yield than the control ones, and in some cases the yields were almost three times higher. However, the optimal compost dose varied, depending on the examined parameter. Based on the evaluation of the experiment results along with rainfall data, it was concluded that, with higher precipitation, the positive effect of the compost application was bigger. The difference between the natural and enriched compost is remarkable, but it has to be mentioned that even the natural compost showed good results; therefore, it is also effective in improving the fertility of grassland.
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The effects of fertilization on the protein related properties of winter wheat
67-69Views:526The yield and quality of wheat are mainly determined by the plant production system, thus we studied the effect of mineral fertilization.
The field trials were set up in 1983 at the Látókép Research Institute of the University of Debrecen. We examined effect of different Nfertilizer doses (60 kg ha-1 N/P/K, 120 kg ha-1 N/P/K) on Lupus, Mv Toldi and GK Csillag's protein properties in 2012. During the tests, three quality parameters were determined: wet gluten content (%), wet gluten spread (mm/h) and gluten index (%). In the experiment the effect of different doses of N-fertilizers significantly influenced by the wet gluten content and gluten index of Lupus.
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Comparative analysis of inoculated soybean in extinguishing soil
113-116Views:612In order to achieve higher yields, better technological methods offered in the current market, which aims to help the producers in the realization. To guarantee good yield because more and more people try with a variety of products, but you have to make the expected impact falls short.In this experiment, the following results were obtained: the soil suspension closely related to the inoculum of seed or seed treatment,which affects the soybean nodule formation, core saturation, and yield and protein-oil indicators. The soil suspension composition may exert positive and negative effects, which depend on how bacterial strains inoculum combined on the soybean seed surface. It is shown in our experiment very well, that between inoculum and inoculum as well as inoculum and seed treatment materials may be antagonism which setback to the soybean nodules formation and the yield, but not worsen the protein-oil indicators.
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Penicillium chrysogenum antifungal protein (PAF) production in transgenic tobacco (Nicotiana tabacum) plant
77-82Views:265Under the „Molecular farming” research program (product vaccines and substances for medical use with gene manipulated plant) in 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture Department of Plant Biotechnology experiments were launched to transform tobacco plant by PAF antifungal protein. Our aim was to learn the transformation technics. We chose the
Nicotiana tabacum and PAF as model systems.
Our work was to express several different paf constructions in plants with nuclear and plastid transformation too. After that we confirmed the presence of paf gene in the level of DNA and RNA. -
Estimation of Inheritabilities from Holstein-Friesian Test Day Data in Hungary
23-25Views:646Recently, test day models (TDM) began to be increasingly used for the genetic evaluation of dairy cattle. The main advantage of the TDM compared with the 305 days lactation yield models is that more effects can be used in the evaluation. Therefore, the TDM is more accurate than the lactation models. The main disadvantage is the increased computational requirement, but this can be offset by improvements in computer capabilities.
The topic of this paper is the use of a fix regression test day model to estimate the inheritabilities of test day data from Hungarian Holstein-Friesian dairy cattle. The inheritability was 0.26 for milk production, 0.2 for fat production, 0.24 for protein production and 0.06 for the somatic cell count. -
Organic soybean production in Hungary
45-52Views:476Organic soy production, particularly for feedstuff in organic animal husbandry, is needed in Hungary and the EU regions. Soybean producing crop area in Hungary has increased in the last years, however, the agroecological potential would allow for a larger soybean production area. This study presents the soybean market in Hungary, and the role of soybeans in protein supply in food and feed, summarising the critical elements of organic soybean production from variety selection to marketing. During the field research, the effects of the agronomical environment on yield potential factors were examined. We aimed to determine how different row spacing and tillage systems affect organic soybean yields of different varieties, with particular attention to the dry region, and determine the specific methods and varieties of soybean that favour these areas. We found that the tillage (plough/grubber), the spacing (wide/narrow), and the variety effects were all significant on morphological variables. The most remarkable difference was seen in plant height and the number of pods per plant, while the number of nodes was also highly impacted by tillage treatment and variety. The nutrition variables were significantly different, mainly as an effect of tillage and spacing interaction with significantly different plant responses of varieties.
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Influencing the growth kinetics of yeast strains with vitamin supplementation
113-115Views:294The aim of the current experiment was to optimize the creation process of single cell protein on plant-based substrate solution with the intention to improve end-product turn out by means of adding vitamin solution. Based on the results of the fermentation processes of yeast strains, it was concluded that the vitamin-supplementation produced its greatest effect on the dry matter production, primarily on the K. marxianus DSM 4908 strain, while it was less beneficent when it comes to the figures of wet cell mass. In addition, it can be assumed that vitamin supplementation increased the maximum specific rate of growth (μmax) and decreased the generation time (tg) significantly. In the case of the K. marxianus yeast strain on corn steep liquor treated with vitamin-supplementation, the highest (μmax) and the lowest (tg) data were observed [(0.226 h-1) and (4.4 h), respectively]. Based on the results it was found that K. marxianus DSM 4908 is expedient to be applied on corn steep liquor medium in order to determine its suitability to produce additive for feeding.
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Phylogenetic studies of soybean pathogen Phoma species by Bayesian analysis
53-61Views:484We carried out phylogenetic study analyzing sequences of genetic markers in the taxonomy of Phoma and Phoma-like fungi. Different species of Phoma and Phoma-like fungi occurring on soybean (Phoma pinodella, Phoma sojicola, Phyllosticta sojicola, Phoma exigua var. exigua) are difficult to identy because of their high morphological and symptomatic similarities.
Twenty-two isolates of nine different Phoma species were obtained from reference culture collections. Seven of them were isolated from soybean, the others were collected from different hosts.
The Phoma isolates were firstly characterised by morphologically, and then we employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1), ITS region, as well as β-tubulin partial sequences as potential genetic markers to infer
phylogenetic relationships among different Phoma species..Finally, their ITS and tef1 sequences were sequenced and analysed by Bayesian approaches.
According to phylogenetic trees inferred by Bayesian analysis of tef1, ITS and β-tubulin sequences, different Phoma species can be separated proving that these phylogenetic markers are well suited for phylogenetic studies of Phoma species. However, the phylogenetic tree does not support the traditional Phoma sections based on morphological characterization.
Bayesian analyses of the three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The molecular data provide evidence for reclassification of formerly mentioned soybean pathogens. -
Application of yeasts fortified with microelements – Review
101-106Views:260Microelements are increasingly becoming into the focus of interest from both a point of view of nutrition science and feeding. An always growing care must be paid to the microelements coverage both in human and animal organisms because of incorrect alimentation habits and
unsatisfactory feedstuff nutrition value. For the increased supply of the micro-nutrients, enrichment or fortification with microelements can not only be realized with traditional foodstuffs and forage but there are already alternative ways such as single-cell proteins from yeasts directly enriched or fortified with microelements for the purpose.
We would like to draw the attention that the production of these items is more favourable in comparison with traditional foodstuffs or forage since yeasts are capable to multiply microelement levels compared to their original state, and establish organic bonds with them.
For this purpose, we explored and analysed the scientific literature, studies and research results on this subject, that is why we stressed the significance of yeasts, the features and health effects of certain microelements, as well as the possibilities for use of yeasts enriched with micro-nutrients. -
The potential use of Pediococcus spp. probiotic in aquaculture: A review
99-106Views:612Aquaculture production has significantly increased over the previous few decades. However, antibiotics have been implemented regularly and extensively to overcome outbreaks of pathogens in aquaculture to cover the human needs for animal protein, leading to the appearance of resistant strains that may cause serious damages in the environment and also human health. In the last few years, the implementation of probiotics as an alternative technique to antibiotics use in fish production has achieved promising results in aquaculture due to their beneficial impact on fish health and growth performance. Among different types of probiotics, the Pediococcus spp. bacteria stand out as a promising probiotic for their beneficial impact to aquaculture. Thus, the current study has been conducted to give an overview about the interactions between Pediococcus spp. and aquaculture. In addition, this review highlights the role of Pediococcus spp in promoting growth performance, improving feed conversion ratios and the intestinal architecture, enhancing the immune response and inhibiting fish pathogens, thereby preventing or at least reducing the use of antibiotics. Practical use of Pediococcus spp. probiotic in aquaculture as feed additives through selected case studies is also considered.
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Reference genes for livestock gene expression profiling – Literature review
81-89Views:563Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments. -
Study of the biodegradation of slaughterhause feather waste by Bradford method
77-81Views:318The 15–20% of the by-products of meat- and poultry industry – that unsuitable for human consumption – contains keratin. The slaughter technology of poultry produces large amount of poultry feather with 50–70% moisture content. This means more million tons annually worldwide (Williams et al., 1991; Hegedűs et al., 1998). The keratin content of feather can be difficulty digested, so physical, chemical and/or biological pre-treatment is needed in practice, which has to be set according to the utilization method. The microbiological and enzymatic degradation of feather to soluble protein and amino acids is a very favourable and relatively cheap opportunity to produce valuable products of the resulting feather. Our applied treatments were based on the determination of the most effective method, which is able to follow the biodegradation of waste poultry feather.