Search
Search Results
-
Changes in the Quality Parameters of Wheat Samples of 16% Moisture Content during Storage
117-121Views:156We have carried out storage experiments with wheat varieties Magvas, Fatima, Mv Emma and Mv Pálma from the 2001 growth year. During the first half of the experiments quality parameters, such as changes in moisture, protein and gluten contents as well as pharinograph readings, falling numbers and sedimentation indices were analysed and tested at an average initial moisture content of 13%.
The findings in the changes in the quality parameters of different wheat varieties with 13% moisture content as a function of storage time duration were published in an earlier paper of mine (12/04/2002).
This paper wishes to give an account of the second part of the storage test when the moisture contents of the different varieties were raised to 16% and the changes in the quality parameters were monitored for seven weeks. The findings show that contrary to what had been experienced in the first half of the trial, there were no changes in the protein contents, pharinograph readings, water uptake or Zeleny counts and falling numbers showed decreases with each of the varieties tested. -
Variation of boza nutrition parameters based on raw materials
67-70Views:463Boza is a ceral based fermented drink. Raw material of boza could be rise, millet, wheat and rye. Because of the diversity of raw materilas and the method of fermentation boza might show varied quality and nutrition value.
Nowadays healthy and conscious nutrition are playing more and more important roles in our lives. People pay an ever growing attention to the nutrition value of products, such as protein- and sugar content, components with antioxidant properties, and the mineral content of the products. In our research we wanted to know how the protein,-, sugar-, total polifenol-, mikro- and macro element content of products change.
-
Association analysis of TNNI1/XbaI polimorphism on carcass quality in hybrid pigs
59-62Views:563The contractile protein, which is encoded by troponin I 1 (TNNI1) gene, is located on the thin filaments of slow fibres in striated muscle. TNNI1 protein is a part of the troponin complex which plays an important role in regulation of muscle contraction by preventing actin-myosin interaction in absence of calcium. According to biological role, this gene can be potential marker for meat production related traits. The aim of this study is to define whether the previously reported gene polymorphism (EU743939:g.5174T>C) is connected with the slaughter traits measured in a standard slaughterhouse of the examined four-line European hybrid. The study included data from 404 gilts and barrows from 2 different samples. The polymorphism was detected using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method with XbaI restriction enzyme. In this study the allele frequencies were found as follows: C: 0.84 and 0.808; T: 0.16 and 0.192. Based on result of the present study no significant impact of polymorphisms on production parameters was found.
-
Isolation of promoters of tissue and ripening-specific strawberry genes by TAIL-PCR and bioinformatic characterization of the sequences
91-99Views:179Isolation of ripening- and tissue-specific promoters has become a very important subject of the genetic regulation and plant physiology research in recent years. It could be possible to reveal the regulation of gene expression, and it may be a very useful approach in the biotechnology. In our work, we have isolated promoter regions of genes exhibiting ripening- and tissuespecific expression in our previous experiments, and the data were
characterized by bioinformatic methods. In the sequence of the ripening-specific Spatula and ACC-oxidase promoters (ACCoxidase is one of the key-enzymes of ethylene biosynthesis, directly related to the process of ripening); we could identify auxin- and ethylene-related cis-regulatory elements. This suggests that there is an interaction between ripening and ethylene-synthesis, in case of non-climacteric strawberry, too. We investigated the promoter regions of three green receptacle-specific genes (putative nitrilaselike protein, Ring transcription factor and an aquaporin protein)
and we could identify several regulatory elements, which refer to hormonal regulation. Additionally, we could find several cisacting elements which associated with stress-responsiveness and endospermium-specific expression. -
The relationship between gluten proteins and baking quality
117-122Views:744Wheat, one of the most important cereals, is grown on the largest area in Hungary. During hydration of storage proteins of wheat – gliadin and glutenin – the gluten complex is evolved. The gliadin is responsible for the extensibility of gluten complex as well as the glutenin for the strength of gluten. The structure, composition and rheological properties of gluten proteins influence significantly the baking quality. The gliadin/glutenin ratio and the quality and structure of glutenin fraction play the most important role in evolving gluten complex. Changes in the steps of breadmaking technology also have effect on the quality of product. Several tests proved that the higher glutenin content increases the strength of dough while the higher gliadin content increases the extensibility of dough and decreases maximum resistance to extension. The monomer gliadins play a great part in plasticity of glutenin polymer. The quality of glutenin fraction significantly influences the evolving gluten complex, because of the spiral structure of glutenin which deforms under stress conditions, then the β-spiral structure resumes their original conformation by releasing from stress.
The final quality of product evolves as a result of complex characteristics of wheat proteins, so detailed knowledge on the roles of different protein compounds is the base of the quality oriented product development. -
Investigation of the β-casein (CSN2) gene polymorphism in goat breed – Review
69-73Views:1550Milk plays an important role in the human nutrition as an important protein and energy source. Β-casein is the most abundant casein fraction in the goat milk. The β-casein gene has at least 9 polymorphism in goat breed (A, A1, B, C, C1, D, E, 0 and 0’). The most important alleles are the zero (0) and zero’ (0’) alleles, because they decrease the quantity of β-casein protein in milk. The lower quantity of the β-casein changes the properties of the coagulation and the allergenin milk. This review is about the β-casein gene polymorphism in goat breed.
-
The variation of breast milk composition during the lactation stage for mothers from the Csík region
93-98Views:295The aim of our research was the examination of breast-milk composition from mothers living in the Csík region and to follow their milk composition variations during lactation. In this article we present the results obtained from three mothers, paying particular attention to essential components. The breast milk samples were collected from healthy mothers with similar habits and age. The milk samples were collected with a hand pump at the
same time after the feeding. The sampling period was from day 5 to the 14th weeks of lactation. The nutrition of mothers was recorded on a questionnaire, completed by the mothers themselves. Comparing our experimental results with data in the literature it was concluded that the milk protein content was very similar to the milk of mothers from other European countries, and is decreased during lactation.
The total saturated fatty acid content was lower, but the palmitic acid content was slightly higher. Regarding the essential fatty acid composition the linoleic- and the arachidonic acid contents were appropriate from a nutritional point of view. The linolenic acid was present in lower amounts, but the docosahexanoic acid was almost undetectable.
The iron and manganese contents were in good agreemen with published results, but the zinc content of the breast milk was lower and its copper content was higher. These differences in milk composition can be explained by the different nutritional habits of our subjects. -
Variations in major quality parameters of forage and medium quality winter wheat varieties in storage
249-254Views:421We analysed five parameters (moisture-, protein content, Hagberg’s falling number, wet gluten content and alveographic W (10-4 J) values) and the microbiological changes of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) during storage, to determine the tendency, type and volume of the of the change of this five qualitative parameters during storage.
We found that the examined winter wheat varieties retained their moisture, protein content and their Hagberg’s falling number, they did not change during storage.
A slight growth could be experienced in the values of wet gluten content for all the four winter wheat varieties in terms of the duration of storage (129 days). This result proved the theory of after-ripening, when gluten percentage improves qualitatively and quantitatively as well. The value of the quantitative growth was about 10% for all the four winter wheat varieties.
We placed a special emphasis on measuring the alveographic W (10-4 J) values during storage. All the four winter wheat varieties showed decreasing values of about 20-40%.
Microbiological examinations on the four winter wheat varieties showed that mould, mould flora and total germ count remained balanced with some slight variations and they did not change in terms of time under optimal storage conditions. -
Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:371The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations. -
Development of maize production technology that increase the efficiency of bioethanol production
17-26Views:402Maize is one of the most important crops worldwide and also in Hungary, it can be utilized for multiple purposes: as a feedingstuff, for human nutrition and for industrial processing. In the last decades, the per ha yield of maize varied greatly in Hungary, between 2004 and 2006, it was 6.82-7.56 t/ha, while in 2007, it was only 3.6 t/ha. Resulting from this, the price of maize became 2-2.5 times higher. The high price hinders bioethanol production. The largest per ton amount of bioethanol, 387 l, can be produced from maize.
In addition to its classical utilization as feed and food, the industrial use (especially for bioethanol production) of maize is increasin.
For industrial production, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2007.
The applied fertilization treatment was N 120, P2O5 80 uniformly, and five different dosages of potassium: K2O 0, K2O 100 (KCl), K2O 100 (Kornkáli), K2O 200 (KCl), K2O 200 (Kornkáli) kg/ha active ingredient. The applied plant densities were 40, 50, 60, 70, 80, 90 thousand plants/ha.
The yield of maize hybrids in the fertilization experiment ranged between 10.53 – 14.62 t/ha. Both regarding the form and dosage, 100 kg/ha Kornkáli proved to be the best potassium treatment. Regarding the inner content parameters, the highest starch content in the average of treatments was obtained for the hybrid PR36K67: 73.57%, and its yield was also the highest, so this hybrid proved to be the most suitable for bioethanol production. The highest protein content was observed for the hybrids KWS 353 (12.13%), which can be favourable for feeding purposes.
Most of the hybrids gave the highest yield at 80 thousand plants/ha plant density, however, hybrids PR36K67 and Mv Tarján achieved the highest yield at 90 thousand plants/ha.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
Study of some cooking and eating quality characters on some Egyptian rice genotypes
77-82Views:292Some Egyptian rice genotypes [i.e. Japonica (Sakha 104), Japonica/Indica (Egyptian hybrid1) and Indica (Giza 182)] were investigated to evaluate the cooking and eating quality characters. High significant differences in grain shape were observed among rice genotypes. Hulling, milling and head rice percentage were higher in Sakha 104 than other rice genotypes, while Indica type (Giza 182) recorded the lowest values in milling and physical characters. No significant differences were found in chemical composition of the three genotypes of rice was recorded, but Giza 182 had the highest protein content. All Egyptian rice genotypes were low in gelatinization temperature and soft in gel consistency. Japonica and Indica rice varieties were low in amylase content, while Japonica/Indica rice variety was intermediate. The use of RVA is considered a good index for palatability evaluation for milled rice flour and starch. The Indica and Japonica/Indica types are low in breakdown viscosity, but higher in cooked pasta than Japonica type. Japonica type recorded the best score in panel test, followed by Indica type, while Indica/Japonica rice variety was the least accepted by Egyptian consumer.
-
The effects of drought stress on soybean (Glycine max (L.) Merr.) growth, physiology and quality – Review
19-24Views:428Abiotic stresses are one of the most limiting factors inhibit plant's growth, leading to a serious production loss. Drought stress is one of the most destructive abiotic stresses and is still increasing year after year resulting in serious yield losses in many regions of the world,
consequently, affecting world’s food security for the increasing world population. Soybean is an important grain legume. It is one of the five major crops in the world, an essential source of oil, protein, macronutrients and minerals, and it is known as the main source of plant oil and protein. Harvested area of soybean is increasing globally year after year. However, soybean is the highest drought stress sensitive crop, the water deficit influences the physiology, production and seed composition of this crop. We introduce a review for literatures concerning the changes of the above traits of soybean exposed to drought stress, with past explanations for these changes. -
Impact of chronic heat stress on digestibility of nutrients and performance of meat type ducks
51-55Views:370The aim of the study is to determine the effect of vitamin and mineral supplement under permanently high environmental temperature (30±1 °C) on the digestibility of nutrients, performance and furthermore the composition of duck meat in the growing period. A total of four hundred mixed sex 14 days old Cherry Valley type hybrid ducks were used for the study. Two experimental diets were formulated in the study (control and vitamin E, C and zinc supplemented diet). Based on the results the following conclusions were drown: the antioxidant defence system plays an important role in the reduction of heat stress generated lipid peroxidation process. Feed additives which have direct or indirect antioxidant effects can reduce the negative effects of heat stress on the ducks performance and meat composition. Digestibility of nutrients (Dry Matter, Crude Protein, Crude Fat) was not affected by antioxidant supplementation under chronic heat stress (30±1 °C). The performance was affected significantly by Vitamin C and E and zinc supplementation under heat stress (P<0.05). In the treated group the daily weight gain (dWG) increased and the feed conversion ratio (FCR) was improved significantly (P<0.05). The energy and protein conversion was decreased also significantly (P<0.05).
-
Influence of nitrogen fertilisation on the technological quality of wheat
47-55Views:359Wheat is one of the most grown crops around the world. Its primary use is in the production of bread, bakery, and confectionery. The provision of essential nutrients, mainly nitrogen, plays a pivotal role in the growth and development of wheat. The wheat varieties used in the experiment are Alfold and Mv. Menrot. The rates of nitrogen used in the experiment are: 0, 200, 400, 600, 800, and 1000 kg N ha-1. The aim of this study is to evaluate the effect of nitrogen fertilisation on technological quality of wheat and to find the appropriate fertiliser rate to reduce pollution. The results indicate that nitrogen fertilisation did not show a significant effect on thousand kernel weight and test weight. However, nitrogen fertilisation significantly affected protein content, gluten content, Zeleny sedimentation index, and the falling number. The higher the nitrogen fertilisation the better the technological quality parameters of the wheat. The protein content, gluten content, Zeleny sedimentation index and the falling number were the lowest at 0 kg N ha-1 followed by 200 kg N ha-1 then 400 kg N ha-1 and the highest at 600 kg N ha-1.
-
The composition of gluten proteins and their effect on the rheological properties of gluten
124-129Views:513Wheat is the major cereal component of bread in the world and is grown worldwide. Of the cereals only the bread wheats – and less the triticale – includes storage proteins that play an important role in the performance of gluten. Proteins of gluten complex may be present in two classes:
− low molecular weight (gliadin-) components, and
− high molecular weight (glutenin-) components.
Gliadins shown appreciable heterogenity and can be separated into 40-50 components with gel electrophoresis. The composition of gliadins is employable for the identification the wheat varieties and to investigate the varieties. In the decreasing electrophoretic mobility sequence may be distinguish α-, β-, γ- and ω-gliadins. A glutenin subunits may be include in two classes:
− high molecular weight glutenin subunits (HMW-GS),
− low molecular weight glutenin subunits (LMW-GS).
Wheat varieties can be identified by glutenin and their quality selection is also possible. The gliadin’s polypeptides encoding genes are located on the short arm of chromosomes 1A, 1B and 1D, 6A, 6B and 6D. Genetic coding for HMW subunits is located on the long arms of chromosomes 1A, 1B and 1D, the LMW-GS are also located on chromosomes 1A, 1B and 1D (Glu-3 loci) near the gliadin-coding loci.
Storage proteins affect the rheological properties of gluten by two factors:
1. The quality and quantity of the protein components of the gluten complex,
2. The interactions between the protein fractions. -
Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:395The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. -
Overview of the evolutionary history and the role in citric acid production of alternative oxidase
83-88Views:293All organisms are exposed to countless environmental effects, which influence in a disadvantageous way their life processes. They continuously adapt to the changing conditions and respond to the stress impacts by defence mechanisms. Through different signal transduction pathways they are able to increase or decrease the expression of their genes and consequently modify their metabolic processes. My interest focuses on alternative oxidase (AOX) enzyme whose expression is often increased under biotic and abiotic stress. The so far proven and putative functions of the AOX play a role in the ability of organisms to adapt to different conditions, such as heavy metals accumulation, pathogenic infection, oxidative stress and lack of oxygen or nutrients.
AOX is a member of the di-iron carboxylate protein family. Members of the di-iron carboxylate protein family are present in all kingdoms of life. They are considered to have ancient origin. It is believed that their sulfide-resistant and oxygen-reducing ability played a role in the survival of organisms during the transition between the anaerobic and the aerobic world. It is assumed that the AOX arose in eukaryotes through a primary endosymbiotic event, and this event made possible the development of mitochondria. Afterwords, vertical inheritance, and secondary and tertiary endosimbiotic events led to its spread among eukaryotes. It is assumed that bacteria obtained AOX by horizontal gene transfer from plants.
AOX-catalyzed alternative respiration plays an important role in the operation of energy-producing and biosynthesizing system of microorganisms. In these cases, the regeneration of reduced cofactors is an essential condition, and therefore may be rate-limiting for biotechnological processes, including the citric acid production.
-
The importance of chickpea (Cicer arietinum L.) and its cultivation in Hungary: A review
71-75Views:255Climate change today is no longer a question for the future. Climate change impacts not only human populations but also plant species, which are increasingly exposed to its negative effects. The increasing number of days of drought, the lack of precipitation and its unfavorable distribution are observed each year, which require adaptation. Chickpea (Cicer arietinum L.) is a drought-tolerant species characterized by deep root system that enables it to withstand prolonged periods without precipitation. Additionally, it is a thermophilic crop and tolerates moderate increases in average temperature." There is no great tradition of its cultivation and use in Hungary, but it has been cultivated by the Iregszemcse Research Institute since the 1970s. In addition to the positive aspects of its cultivation, it also has good nutritional values, outperforming in some parameters the beans (Phaseolus vulgaris L.) and peas (Pisum sativum L.) what are popular in Hungary. Its high protein and crude fibre content allows it to be used not only for human consumption but also for animal feed. The aim of this review is to describe the importance of chickpea and to identify the advantages and disadvantages of its cultivation.
-
The effect of NPK fertilization and the plant density on maize yield and bioethanol production
13-18Views:392For industrial (bioethanol) production of maize, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2008.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
Sorghum and millet as alternative grains in nutrition
91-95Views:532Sorghum (Sorghum bicolor L.) and millet (Panicum miliaceum L.) are the fifth and sixth most important cereal crops in the world. Gluten-free grains, therefore persons with coeliac disease could consume them also. In addition, they have a lot of positive effects due to their phenolic compounds (phenol acid, flavonoid, tannin). The total phenol content of sorghum is high, but Panicum miliaceum and Eleusine coracana have higher antioxidant activity. Fiber and mineral contents are also high, the protein contents are also higher than in standard cereals. Sorghum use is similar to corn: starch, glucose, syrup, and oil can be produced. Moreover, it can be used in preparing whole grain products, bread, pancake, dumpling, mush, cake, pasta and beer from sorghum. Broom and forage are also can be prepeared from them. Millet used such as mush, steamed food, cake, bread, alcoholic and non-alcoholic beverages.
-
Perspectives of the livestock sector in the Philippines: A review
175-188Views:4220The Philippine livestock industry is a vital component in the country's agricultural sector. It contributes around 18.23 percent of the gross output value in agriculture and provides livelihood to many people living in rural areas, signifying its importance with regard to the country’s economy. The constantly increasing demand for animal protein associated with the increase of the world’s population led to the intensification of livestock production, realized through the various initiatives implemented by the government, such as the import of high producing purebreds of various species. This strategy greatly influenced the country's animal genetic resource's diversity and increased food animals' population; however, it impacted the native breeds of food animals. Understanding the characteristics, performance and value of native and exotic breeds is essential to prioritize their livestock industry existence. Thus, this paper aims to characterize various native and exotic breeds of livestock in the Philippines and assess the current conservation of native animals.
-
The examination of poultry feather digestility for biogas production
113-118Views:326The aim of this research was the elaboration of the technological parameters of biological digestion and biogas production from poultry feather produced in large quantities by slaughterhouses. Feather protein was digested by Bacillus licheniformis, keratin desintegrator bacteria. Investigations focused on the optimalization of parameters influencing poultry feather biodegradation. The optimal range of pH, temperature, feather size and bacillus:feather ratio were determined in the experiments as well as the analysis of relationship between the examined parameters. In order to be able to track the dinamics of the biodegradation, we determined the extintion level of the liquid phase of the biodegraded material in the different experimental treatments. The results showed that the rate of hydrolisis was significantly higher in the treatments with bacteria than in the treatments without it. The most extensive digestion were observed in case of 1:3 feather:water ratio. The highest intensity of feather digestion were detected in the treatment with 1% microbe ratio.
-
Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:626The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group. -
Study of alternative oxidase as possible molecular marker for phylogenetic analysis of the Botrytis cinerea
127-132Views:302Botrytis cinerea (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is able to attack several economically important plants causing gray rot. Botrytis cinerea species complex includes two cryptic species (B. cinerea and B. pseudocinerea) that tolerate fungicides differently. On the basis of classical taxonomic markers, the two related species are very difficult to be distinguished; therefore, their separation is usually performed using molecular methods based on the time-consuming molecular analysis of several markers. Our goal was to find markers, which are suitable for the differentiation. Testing the nucleotide sequences of the alternative oxidase encoding gene, B. cinerea and B. pseudocinerea strains were clearly differentiated. Moreover, the analysis of the protein sequences of the enzyme with the maximum likelihood method reflected well the taxonomic relationships of the different fungi.
-
Overview of test methods used to classify wheat flour and bread samples – REVIEW
27-34Views:431In Hungary, common wheat (Triticum aestivum ssp. vulgare) is of good quality and world famous. In addition, it plays an important role in the human diet. The classification of flours ground from wheat is quite decisive and there are several methods for its examination. The most important flour testing requirements include moisture content, protein content gluten properties and the most important bakery value number. The measured characteristics give us the opportunity to conclude about the properties of the dough, and then bakery products. Several dynamic and static methods have been developed to study the physical properties of dough. The evaluation of products can be carried out in several respects with the help of a baking test. The multitude of methods currently used to qualify flour, dough and finished products also proves that the overview of the methods is quite topical.