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Penicillium chrysogenum antifungal protein (PAF) production in transgenic tobacco (Nicotiana tabacum) plant
77-82Views:264Under the „Molecular farming” research program (product vaccines and substances for medical use with gene manipulated plant) in 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture Department of Plant Biotechnology experiments were launched to transform tobacco plant by PAF antifungal protein. Our aim was to learn the transformation technics. We chose the
Nicotiana tabacum and PAF as model systems.
Our work was to express several different paf constructions in plants with nuclear and plastid transformation too. After that we confirmed the presence of paf gene in the level of DNA and RNA. -
Comparison of chemical parameters of enzyme active and inactive malt types
161-166Views:420Nowadays there is an increasing emphasis on the use of raw materials. Typically, raw materials – in this study malt – are used in animal feeds and used in the brewing industry. However, in terms of quality (eg. high fibre content), these can be included in human nutrition, we have limited information on this possibility. The aim of our work was to compare different malt flours and examine the possibility of using malt in the baking industry. We were to investigate some of the most relevant parameters, such as dietary fibre content, crude protein content, fat content, carbohydrate content, dry matter content, moisture content, salt and energy content. In the future, we aim to conduct a research on some of these parameters with different malt types as the brewing industry uses novel ingredients different cereals, pseudocereals such as amaranth (Amaranthus spp.), oat (Avena sativa L.), quinoa (Chenopodium quinoa Willd.) in addition to the spring barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.). Based on brewing studies, malt has a high fibre and protein content. Having these advantageous qualities, malt should be part of humans’ healthy diet. Using malt flour in the baking industry can be a new direction which can lead to creating a healthier lifestyle and healthier eating habits than suggested by the WHO (World Health Organization).
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Effects of agrotechnical factors on the quality and quantity of yield in winter wheat production
69-75Views:441The present study was conducted to determine the effect of basic agrotechnical factors on the yield and quality of winter wheat. Two experiments were set in 2017/2018 growing season, where we studied the influence of different forecrops, fertilizing treatments and cultivars. 204 samples were measured with Single Kernel Characterization System and NIR grain analyser to determine protein (NIR-P), wet gluten (NIR-WG), Hardness Index (HI), kernel weight (KW) and kernel diameter (KD). Fertilizing had a significant effect on yield, KW, HI, NIR-P and NIR-WG, except KD. N90PK dosage was enough to realize yield potential for 6 out of 9 cultivars, but considering protein content N150PK dosage was needed. The forecrop had no significant influence on yield, KW, KD or HI, however sweet corn as previous crop had significant improving effect on NIR-P and NIR-WG compared to sunflower as forecrop. According to our data of correlation analysis, no negative relationship was found between yield and NIR-P, however HI was in medium positive correlation with NIR-P. The variety Vyckor had the highest yield, but in quality aspect, the highest NIR-P and NIR-WG values belonged to KG Kunhalom variety.
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Effect of sex on some composition traits of wild boar (wild pig) meat
25-29Views:655The authors examined the nutrition value of the meat of shot wild boars (wild pigs) (n=66) from three wild boar enclosures with different feeding intensity and also the technological properties of the meat. Samples were taken immediately after the evisceration. Considering the storing and processing properties of game meat the samples were taken from m. serratus anterior. As for dry matter examination results, the highest values were measured in case of semi-intensively fed wild boars, then followed the data from the samples of intensively and extensively fed wild boars. The fat content from the meat samples of intensively and extensively fed wild boars proved to be lower while in case of the semi-ntensively fed wild boars it was higher. In females the dry matter content, while in males the fat content was higher. As for the protein content there were no differences in either the feeding groups or in the genders. It was only the water holding capacity of the samples from the meat of the females from semi-intensive feeding intensity wild boar enclosure that fell in between normal values.
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Challenges and limtations of site specific crop production applications of wheat and maize
101-104Views:244The development and implementation of precision agriculture or site-specific farming has been made possible by combining the Global Positioning System (GPS) and the Geographic Information Systems (GIS). Site specific agronomic applications are of high importance concerning the efficiency of management in crop production as well as the protection and maintenance of environment and nature. Precision crop production management techniques were applied at four locations to evaluate their impact on small plot units sown by wheat (Triticum aestivum L.) and maize (Zea mays L.) in a Hungarian national case study. The results obtained suggest the applicability of the site specific management techniques, however the crops studied responded in a different way concerning the impact of applications. Maize had a stronger response regarding grain yield and weed canopy. Wheat was responding better than maize concerning plant density and protein content performance.
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The significance of biological bases in maize production
61-65Views:309The comparative trial has been set up in the Demonstration Garden of the Institute of Crop Sciences of the University of Debrecen, Centre for Agricultural and Applied Economic Studies, Faculty of Agricultural and Food Sciences and Environmental Management in 2012, with 24 hybrids with different genetic characteristics and growing periods. The soil of the trial is lime-coated chernozem, with a humus layer of 50–70 cm.
The weather of the trial year was quite droughty; the monthly average temperature was 3–4 oC higher than the average of 30 years. High temperature, together with lack of precipitation occurred during the most sensitive phenophases of maize (flowering; fecundation, grain saturation).
The following characteristics have been observed: starting vigour, date of male and female flowering, plant and cob height, dry-down dynamics during maturation and the change of yield composing elements has also been quantified. The yield was recalculated to 14% moisture content grain yield after harvesting.
The beginning of the growing period was advantageous, therefore the analysed hybrids could grow a high (above 300 cm) and strong stem. The yield of the hybrids changed between 10.33 and 11.87 t ha-1, but as a result of the unfavourable climatic extremes, their genetic yield potential prevailed only at a rate of 30–40%. However, moisture content by the time of harvesting was good despite its early date (12th September); it remained under below 14% in most cases. Dry-down was measured on a weekly basis between 14th August and 5th September.
The analysis of the qualitative parameters of the maize hybrids (protein %, oil % and starch %) resulted in significant differences. The most significant difference has been observed in the case of protein content (LSD5%=2.01). Oil content was the most advantageous in the case of hybrids belonging to the mid-late growing group (FAO 400). The X9N655 and 36V74 hybrids had the highest oil content (around 4%), while hybrids P9915 and 37F73 had significantly lower oil content. Starch content was above 70% in the case of every hybrid.
Hybrid selection is highly important in terms of yield and yield security of maize, as well as the application of modern biological fundamentals and hybrid specific technology for the improvement of the level of cultivation technology.
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Investigation of the Quality of Winter Wheat in a Sulphur Fertilisation Experiment, 2001/2002
153-156Views:434We have started a small parcell and a factorial S fertilization experiment with winter wheat in the 2001/2002 cropping year to examine its effect on yield and quality. The scene of experiment was the Latokep Experimental Station of the DE ATC (calcareous chernozeem) in case of small parcell examination and the Agricultural Company of Felsőzsolca (brown forest soil) in case od factorial examination.
The protein and gluten content of the grain was investigated with PerCon Inframatic 9001 NIR Analyser, then we have measured these parameters with PerCon 8620 infra appliance. After the milling we measured the following parameters: glutenindex, farinographic parameters (farinographic index, water absorption capacity, dough development time, stability, softening, extension) and valorigraphic index.
Based on the results there’s no justified relation between the fertilization and the protein and gluten content. The valorigraphic index of the samples taken from Felsőzsolca factory characteristically increased as a result of the S-fertilization. In the small-parcell experiment the values of the water absorption capacity, the dough development time and the softening parameters from the valorigrphic parameters depended significantly from the mineral treatments. Signifikant quality improvement wasn’t experieced.
There was sampling in all of critical phenophase (…). The green plant samples were examined on element content with ICP-OES. These measurements are currently in progress. -
Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:281The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:808The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Preliminary results of the combined production of duckweed Spirodela polyrhiza and common carp (Cyprinus carpio) in an aquaponic system
83-89Views:423As a result of population growth, increasin amount of food is needed, and agriculture is under an increasing burden to meet these needs. Traditional agriculture is often attacked due to its environmental impact. We must find alternative, environmentally friendly ways to produce more food. Aquaponics is a closed system in which we can produce both fish and plants at the same time. Duckweed species are small, aquatic, floating plants belonging to angiosperms. It can potentially be an alternative protein source, due to its high protein content, good amino acid supply, and rapid growth. Under suitable conditions, it doubles its weight in 2–4 days, and can reach a yield of 30 t ha-1 year-1in dry matter. It forms a carpet on top of the water and can be found in slow-moving or still waters. Since they are resistant to a wide range of nutrient concentrations, they are also suitable for cleaning wastewater (such as eutrophicated lakes, sewage reservoirs, liquid manure storage). Fish feed is the primary nutrient source for aquaponic systems, which usually contains fishmeal. If duckweed can be used as an alternative for fishmeal in the feed, it could improve the sustainability of the aquaponic and aquaculture systems. In this study, the aim was to develop an optimal harvesting protocol for duckweed Spirodela polyrhiza under aquaponic conditions. In a four weeks experiments, four harvesting protocols were set up, a control where only biomass measurements were made, a 25% group where 25% of the biomass at the time of measurement was harvested, and a 50% and a 75% group where at the time of measurement 50% and 75% of the biomass was harvested. Three replicates were used per treatment. We weighed the biomass every week and removed the amount corresponding to the group. Based on the preliminary results, it can be said that more biomass was obtained in the groups with the 25% harvesting protocol and the control group.
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The effect of irrigation on the yield and quality of maize (Zea mays L.) hybrids
143-147Views:358In this study, the effect of water supply on the quality and productivity of different maize hybrids was observed.
Maize production is influenced by many agro-technical factors such as nutrient supply, plant density, environmental factors, water supply and temperature. Good soil quality and adequate technology significantly reduce the unfavourable effect of crop year. The impact of fertilization, crop rotation, irrigation and plant density on maize yield was greatly affected by crop year and crop rotation. The main constituent (69–75% dry matter concentration) of maize seed is starch, however, its increase can be achieved only to a limited extent, as increasing the starch content will reduce other parameters, especially protein content. Significant nutrient content improvements can be achieved by appropriate hybrid-site connection and the use of adequate technology. Protein content reduction with optimal water supply can be positively influenced by the appropriate nutrient supply for the hybrid.
The experiments were carried out at the Látókép Experimental Site of Crop Production on calcareous chernozem soil. The weather of the examined year, which was partly favorable for maize and partly unfavourable in other respects, was also reflected in the development of maize and yields achieved.
In 2019, we were examining the hybrids of Kamaria (FAO 370), P 9903 (FAO 390), DKC 4351 (FAO 370) and KWS Kamparis (FAO 350–400). Due to the dry soil condition, sowing was delayed, however, the hybrids emerged ideally because rain arrived soon after sowing, which facilitated initial development. Young plants evolved rapidly and dynamically in the case of all hybrids. In terms of heat-demanding bread, the month of May was unfavourable to temperatures below the annual average, but the higher amount rainfall helped the development. However, due to the drier period in early July, 25–25 mm of irrigation water was applied to half of the experimental areas on July 1 and 15.
The aim of our research was to determine the best population density for hybrids under favourable soil conditions (calcareous chernozem soil). In both irrigated and dry conditions, a crop density of 75,000 crops were the most favourable for the Kamaria hybrid. The P9903 hybrid in the case of a crop density of 85 crops proved to be ideal and the DKC4351 had an optimal population density of 95,000.
However, it should be taken into consideration that, in the case of soils with poor water management, the drought sensitivity of the crop stand may increase at a population density of 95,000.
As a result of irrigation, yield increased and the difference between the examined plant numbers decreased. The yield growth was relatively moderate (341 kg ha-1 – 1053 kg ha-1), which makes the economicalness of irrigation doubtful in the given year.
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Changes in the Quality Parameters of Wheat Samples of 16% Moisture Content during Storage
117-121Views:156We have carried out storage experiments with wheat varieties Magvas, Fatima, Mv Emma and Mv Pálma from the 2001 growth year. During the first half of the experiments quality parameters, such as changes in moisture, protein and gluten contents as well as pharinograph readings, falling numbers and sedimentation indices were analysed and tested at an average initial moisture content of 13%.
The findings in the changes in the quality parameters of different wheat varieties with 13% moisture content as a function of storage time duration were published in an earlier paper of mine (12/04/2002).
This paper wishes to give an account of the second part of the storage test when the moisture contents of the different varieties were raised to 16% and the changes in the quality parameters were monitored for seven weeks. The findings show that contrary to what had been experienced in the first half of the trial, there were no changes in the protein contents, pharinograph readings, water uptake or Zeleny counts and falling numbers showed decreases with each of the varieties tested. -
Variation of boza nutrition parameters based on raw materials
67-70Views:463Boza is a ceral based fermented drink. Raw material of boza could be rise, millet, wheat and rye. Because of the diversity of raw materilas and the method of fermentation boza might show varied quality and nutrition value.
Nowadays healthy and conscious nutrition are playing more and more important roles in our lives. People pay an ever growing attention to the nutrition value of products, such as protein- and sugar content, components with antioxidant properties, and the mineral content of the products. In our research we wanted to know how the protein,-, sugar-, total polifenol-, mikro- and macro element content of products change.
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Association analysis of TNNI1/XbaI polimorphism on carcass quality in hybrid pigs
59-62Views:563The contractile protein, which is encoded by troponin I 1 (TNNI1) gene, is located on the thin filaments of slow fibres in striated muscle. TNNI1 protein is a part of the troponin complex which plays an important role in regulation of muscle contraction by preventing actin-myosin interaction in absence of calcium. According to biological role, this gene can be potential marker for meat production related traits. The aim of this study is to define whether the previously reported gene polymorphism (EU743939:g.5174T>C) is connected with the slaughter traits measured in a standard slaughterhouse of the examined four-line European hybrid. The study included data from 404 gilts and barrows from 2 different samples. The polymorphism was detected using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method with XbaI restriction enzyme. In this study the allele frequencies were found as follows: C: 0.84 and 0.808; T: 0.16 and 0.192. Based on result of the present study no significant impact of polymorphisms on production parameters was found.
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Isolation of promoters of tissue and ripening-specific strawberry genes by TAIL-PCR and bioinformatic characterization of the sequences
91-99Views:179Isolation of ripening- and tissue-specific promoters has become a very important subject of the genetic regulation and plant physiology research in recent years. It could be possible to reveal the regulation of gene expression, and it may be a very useful approach in the biotechnology. In our work, we have isolated promoter regions of genes exhibiting ripening- and tissuespecific expression in our previous experiments, and the data were
characterized by bioinformatic methods. In the sequence of the ripening-specific Spatula and ACC-oxidase promoters (ACCoxidase is one of the key-enzymes of ethylene biosynthesis, directly related to the process of ripening); we could identify auxin- and ethylene-related cis-regulatory elements. This suggests that there is an interaction between ripening and ethylene-synthesis, in case of non-climacteric strawberry, too. We investigated the promoter regions of three green receptacle-specific genes (putative nitrilaselike protein, Ring transcription factor and an aquaporin protein)
and we could identify several regulatory elements, which refer to hormonal regulation. Additionally, we could find several cisacting elements which associated with stress-responsiveness and endospermium-specific expression. -
The relationship between gluten proteins and baking quality
117-122Views:744Wheat, one of the most important cereals, is grown on the largest area in Hungary. During hydration of storage proteins of wheat – gliadin and glutenin – the gluten complex is evolved. The gliadin is responsible for the extensibility of gluten complex as well as the glutenin for the strength of gluten. The structure, composition and rheological properties of gluten proteins influence significantly the baking quality. The gliadin/glutenin ratio and the quality and structure of glutenin fraction play the most important role in evolving gluten complex. Changes in the steps of breadmaking technology also have effect on the quality of product. Several tests proved that the higher glutenin content increases the strength of dough while the higher gliadin content increases the extensibility of dough and decreases maximum resistance to extension. The monomer gliadins play a great part in plasticity of glutenin polymer. The quality of glutenin fraction significantly influences the evolving gluten complex, because of the spiral structure of glutenin which deforms under stress conditions, then the β-spiral structure resumes their original conformation by releasing from stress.
The final quality of product evolves as a result of complex characteristics of wheat proteins, so detailed knowledge on the roles of different protein compounds is the base of the quality oriented product development. -
Investigation of the β-casein (CSN2) gene polymorphism in goat breed – Review
69-73Views:1550Milk plays an important role in the human nutrition as an important protein and energy source. Β-casein is the most abundant casein fraction in the goat milk. The β-casein gene has at least 9 polymorphism in goat breed (A, A1, B, C, C1, D, E, 0 and 0’). The most important alleles are the zero (0) and zero’ (0’) alleles, because they decrease the quantity of β-casein protein in milk. The lower quantity of the β-casein changes the properties of the coagulation and the allergenin milk. This review is about the β-casein gene polymorphism in goat breed.
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A review on fish in vitro digestion studies
55-63Views:150(1) Background: In vitro experiments in fish have been developed to search for dietary substitutes of fish meal in aquafeeds by measuring the digestibility of various feedstuffs by simply simulating the conditions of the stomach or portions of the digestive tract. In vitro digestion studies involving mainly commercial fish species have been conducted to determine the digestibility of conventional and alternative ingredients in fish diets using diverse digestion models. However, there remains a significant knowledge gap, particularly regarding the enzyme functionality, digestion mechanisms in many fish species, and the factors influencing enzymatic activity. This review article is focused on the importance of the use of different enzyme sources in the in vitro digestion model to predict protein hydrolysis.;(2) Methods: For this review, a comprehensive analysis of articles was conducted to gain insights into diverse enzyme interactions and feed evaluation assessments, from the nutritional science and biotechnology sector.; (3) Results: A total of 36 peer-review papers, which include original, and review articles were selected.; (4) Conclusions: In vitro digestion studies offer a valuable tool for evaluating the digestibility of conventional and alternative feed ingredients, helping to identify sustainable dietary substitutes for fish meal while reducing dependence on live animal trials.
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Possibilities of Growing Artichoke (Cynara scolymus L.) in Hungary
126-130Views:210The domestication of choice broadening, special vegetable species offers new market possibilities in Hungary. The production of artichokes has good perspectives due to its high protein, vitamin and mineral contents and its wide usability. According to the literature, in Hungary it is worth examining the annual production technology, which has yet to be researched in our country. The basic aim of our work was to establish the annual production of artichoke in Hungary with scientific research.
In the experiment, 5 Italian sorts (Valetta di Roma, Gobbo di Nizza, Violetto di Romagna, Violetto S. Francesino and Precoce di Romagna), 1 American sort (Green Globe) and 1 Egyptian sort (Balady) were used. For one half of the plants, we tried to bring on bud-production appearing in the first year only with early planting, while we used also a gibberellic acid hormone treatment for the other half.
Only the sort Green Globe had yields in appropriate quantity (21.4 t/ha) and quality. The low yield and inequable quality of buds of the Italian and Egyptian sorts refers to the fact that these sorts are not applicable for annual production in Hungary. Although the gibberellic acid treatment resulted an earlier harvest, increased the equality of buds and increased the proportion of stems producing buds except for the sort Green Globe, overall it did not have a positive effect. The hormone treatment fastened the physiological processes of the plants, thereby it induced early insenescence and lignifying of the buds. The high temperatures during the bud-formation period largely increased the ratio of flabby, over-blowing buds, so the planting must be timed in the future that the harvest period is in early automn or late spring, early summer months.
According to our results, globe artichoke can become a perspective culture by applying cold-resistant sorts with short growing seasons, which can be produced as annual crops without hormone treatment by the appropriate timing of bud-formation. -
Investigation of intersexual polled goats
11-15Views:356The sex reversal may occur in all mammalian species, but is connected to a favourable trait – the polledness – only in the goat. Later abnormal sex ratio was noticed in these goat populations, in which a part of the phenotypically male individuals was sterile. These males have two X chromosomes. In goats the PIS (Polled Intersex Syndrome) mutation is responsible for the absence of horns in homozygous and heterozygous individuals. This same mutation causes a female-to-male sex reversal, but only in the homozygous polled genetic female goats. The PIS mutation inhibits the expression of the FOXL2 gene which is responsible for ovary development, and a protein encoded by this gene inhibits the activity of the Sox9 gene. The Sox9 gene stimulates the development of the cells of the testis. When the FOXL2 gene is inhibited, the Sox9 gene is activated and transforms the ovary cells into testis cells. In our article we briefly introduce the morphological and chromosome investigations of three intersex individuals we found.
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Investigation of the production parameters, nutrient and mineral composition of mealworm (Tenebrio molitor) larvae grown on different substrates
129-133Views:508During the rearing of mealworm (Tenebrio molitor) larvae, the optimisation of the growing substrate has a particular importance. The application of the appropriate substrate is a fundamental pillar for intensive and safe production. The requirements for substrate include the lack of toxins, high nutrient and micro-macro element content. The aim of this study was the evaluation of the effects of different substrates on the production parameters, nutrient and mineral composition of the mealworm larvae. The experiment lasted through 14 days. 5 treatments were set up at, where the variable was the substrate. The test system consisted of 25 units (5 treatments and 5 replicates). 10 mealworm larvae per unit, (total of 250 larvae) were used at the beginning of the experiment. Regarding the substrates, our study included sweet potato (SP), may turnip tuber (MT) and may turnip leaf (ML). In addition to the plant by-products, wheat flour (WF) and wheat semolina (WS) were used as control substrates. Trace element uptake and production parameters of the larvae were determined at the end of the experiment. Regarding the production parameters, it can be stated that the wheat semolina (0.081 ± 0.005 g) and wheat flour (0.069 ± 0.007 g) substrate used as control gave the best results for the final body weight. In terms of plant raw materials and by-products, sweet potatoes (0.063 ± 0.007) can only be recommended as substrate, while may turnip tuber and may turnip leaf produced significantly lower final body weight results (MT=0.034 ± 0.002 g ; ML= 0.036 ± 0.002 g). The nutrient composition of the larvae was not affected by the substrate, these results confirmed the high protein and fat content reported in the literature. The results with the production parameters were contradicted by the micronutrient content. Larvae reared on may turnip leaf (ML) and tuber (MT) showed the highest values for most of the macro- and microelements (potassium, calcium, magnesium, iron, zinc, copper) tested.
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Single nucleotide polymorphism analysis in meat-production related genes in broiler chickens
79-82Views:303In broiler chickens, the intensive selection for growth rate, feed efficiency, body composition (breast muscle weight) traits in the last decades was successful. To improve economically important characteristics, it is possible to use molecular markers associated with meat production traits. The aim of this study was to examine genotype polymorphisms in ROSS 308 broilers for thyroid hormone responsive Spot14α, insulinlike growth factor 1 (IGF1), IGF-binding protein 2 (IGFBP2), somatostatin (SST) and prolactin (PRL) genes. A further goal of this investigation was to study the relationship between the polymorphisms and phenotypic characteristics.
In the investigated broiler population, the frequency for CC homozygous genotype was 0.77 in Spot14α (AY568628), AA homozygous genotype was 0.80 in IGF1 (M74176), GG homozygous genotype was 0.85 in IGFBP2 (U15086), DD homozygous genotype was 0.60 in PRL (FJ663023 or FJ434669). Only the AA homozygous genotype was found in SST (X60191). Chickens with AC genotype in Spot14α, and with GG genotype in IGFBP2 had higher body weight (BW) and carcass weight (CW), compared to CC and GT genotypes. However, the differences were not significant (P>0.05). There was significant association (P<0.05) between PRL genotypes and body and carcass weight, where chicken with homozygous DD surpassed individuals with homozygous II genotypes.
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The impact of applying foliar fertilizers on the health condition of maize
105-109Views:402The nutrient supply of plants is becoming more important in plant protection since the appropriate condition of plants can be ensured by optimal and satisfactory nutrient supply to avoid nutritional deficiency diseases. Due to the extreme weather conditions, plants are forced to face several stress factors, which leads to deterioration of the plant health. The increasing occurrence of droughts poses threat to nutrient uptake through the roots since all the nutrients can be accessible to the plants only if they are in dissolved form in the soil – which is not possible in the absence of water. Therefore, the importance of foliar fertilizer is becoming a more and more significant part of the nutrient supply, because with the help of this technology the development of any nutrient deficiency can be prevented.
In this experiment, we focused on the efficiency of two different foliar fertilizers on maize. Foliar fertilizers were applied two times, once in the stage with 8 leaves and tasseling phenophase of the maize. To verify the efficiency of the foliar fertilizers, the chlorophyll content of untreated and treated plant’s leaves was measured after each application. Moreover, the length and diameter of maize cobs, thousand kernel weight, protein, oil and starch content were also measured, and the results were compared to the untreated (control) ones. According to the results, in all aspects significant differences were observed and due to the laboratory analysis of leaves, in the case of magnesium and zinc supply the foliar fertilizers were able to prevent the development of nutrient deficiency.
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Investigation of the effect of allithiamine-enriched feed on the poultry gut microbiome composition and resistome
149-155Views:274Over the past 20–25 years, the poultry industry has evolved into a specific protein production system. However, the stress resulting from intensive rearing practices has led to numerous negative consequences, making the optimisation of livestock gut microbiome composition crucial for mitigating these effects. Advancements in modern molecular biology methods have brought attention to the impacts of nutrients on gut microbiota. In our study, we extensively investigated the changes induced by feed formulations rich in phytonutrients on the gastrointestinal microbiota of livestock using targeted 16S rRNA amplicon sequencing. Our objective is to examine how the developed feed prototype affects the composition of core microbiomes in raised poultry, community diversity, and the resilience of complex microbial networks. We seek correlations between biological livestock and environmental samples to identify which community constituents, in what proportions and occurrences, may play a role in the development of specific diseases. Based on our measurement results, it can be asserted that allithiamine positively modulated "beneficial" community constituents. Beyond the impact of allithiamine-enriched feed rich in phytonutrients, the composition of the microbial community in the poultry gastrointestinal tract is significantly influenced by the age of the birds. Furthermore, due to the presence of multi-drug-resistant pathogens in environmental samples from livestock facilities, appropriate transmission risk management measures are of paramount importance.
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Changes in the Composition of the Milk of Racka Sheep During Lactation
24-28Views:350We examined the changes in the composition of the milk of 36 Racka sheep during lactation. As milking of Racka sheep is not performed anywhere in the country, the test was restricted to the examination of milk components. However, quantity could not be measured. We analysed the fat, protein, lactose, carbamide and somatic cell count content of the milk from sampling. By means of correlation analysis, milk components were compared to the age of ewes, then the number of lactation days. With the advancing of the lactation and by analysing the milk components, we found differences between the two colour-variants of Racka sheep. Additional trials are required to decide whether the differences between the two colour-variants may be considered as constants, or can be attributable to the relatively small number of samples.