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Soil microbial biomass and community responses to long-term tillage and fertilizer regimes in corn under corn-winter wheat rotation
33-41Views:119Agrotechnical interventions, namely, tillage and fertilization have a great influence on soil microbial activities and biomass, hence it is important to investigate their effect in long-term experiments. This study aimed at evaluating the impact of long-term tillage and NPK mineral fertilizer application on soil microbiological parameters in corn grown under corn-winter wheat rotation. The soil samples were collected in June of 2024 from the long-term experiment of the University of Debrecen at Látókép established in 1991. The treatments included control (no fertilizer), NPK fertilization (160 kg ha-1 N, 60 kg ha-1 P2O5, 90 kg ha-1 K2O) and tillages (Moldboard tillage-MT, Strip tillage-ST and Ripper tillage-RT). The soil microbial biomass and composition of its community (Actinomycetes, saprophytic fungi, Gram-positive bacteria, Arbuscular mycorrhizal fungi, Gram-negative bacteria) was measured by PLFA analysis of soil extract. Results indicate that NPK fertilization affected only the biomass of Arbuscular mycorrhizal fungi and Actinomycetes, whereas tillage greatly influenced the soil microbial biomass and community composition for all the microbial groups. Generally, the microbial biomass and community composition were highest under RT followed by ST and lowest under MT. In conclusion, conservation tillages are more favorable for soil microbial life than conventional tillage.
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The influence of crop rotation and nutrition regime in winter wheat on the phytomass remains and the deposits of weed seeds remained in the ground
10-13Views:258An important problem refers to soil weeds seed bank with special reference to brown luvic soils and relationship between vegetal remains
(roots + stub) and nutrition regime – crop rotation plant. A great number of seeds can be found in first 20 centimeters of soil (plowing
layer). This fact causes a high weeds density, which has a negative effect on the cultivated plants leading to partial or total crop loss. -
Effect of feeds with different crude fiber content on the performance of meat goose
5-8Views:945In the last 50 years, poultry meat production has increased dynamically. The role of crude fiber content in feed is unclear based on a small amount of literature about goose feeding.
The aim of theis experiment was to determine how various crude fiber content (55; 60; 65g crude fiber/kg feed) influences the performance of meat geese in the second phase of the rearing period. 150 goslings (3 treatments, 5 cages/treatment, 10 birds/cage) were included in the test. The experiment started and finished at the age of 21st and 63rd day, respectively.
The obtained results suggest that various crude fiber content did not influence the performance of meat geese significantly; however, a feed of 55g crude fiber/kg resulted in better fattening results (final body weight, body weight gain, specific feed protein and energy conversion rate). In addition, compound feed of 65g crude fiber/kg proved more favourable with respect to cost-efficiency. Based on the obtained results so far, further models and farm experiments are required.
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The Effect of the Mineral Content of the Leg-Horn and the Age of Sheep on the Mechanical Parameters of the Horn
42-45Views:232Species and individual animals with hard leg-horn have higher resistance against foot diseases. The reason for this is the fact, that bacteria can penetrate the hard leg-horn with more difficulty than the soft leg-horn, and in this way it is also more difficult for them to cause an illness. From among the mechanical parameters of the leg-horn the P = 0.1% negative linear correlation between the hardness and the water contents of the horn is significant. There is a positive linear correlation between the shock resistance and the water contents of the leg-horn. In the case of air-dry horn (with less than 8% water contents) there is a positive linear correlation between the Ca- and Zn contents as well as the Ca:P ratio and the hardness of the horn. The results suggest that the wider the Ca:P ratio of the horn, the harder the horn is, while the water content is the same. The leg-horn of the Hungarian Merino ewes have larger Ca contents and wider Ca:P ratio than juvenile animals have. This is why the juvenile animals with a softer leg-horn can be more susceptive to foot diseases than the older animals.
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Examination of the Effect of Transaction Costs in the Hungarian Beef Sector
212-223Views:230technical and economic characteristics, which give rise to high levels of uncertainty and greater control in the supply chain. In order to investigate the role of different transaction costs in marketing behavior, we carried out research in the central region of Hungary among beef retailers and wholesalers. This research is based on primary data collection and examines the motivation of choices in the beef sector on distinction among different marketing channels and the role of transaction costs in procurement. Since this case can be regarded as a qualitative choice situation the hypothesis that transaction cost’s variables are significant is judged by the application of multinomial logit model in order come up with the variables that can influence the supply chain structure and the choice of different marketing channels. This analysis enabled us to explore the structure in data and confirm or reject the expected interrelations of causative variables. Our
1 A szerző témavezetője Dr. Fertő Imre.
2 A kutatás az OTKA F038082 sz. „Vertikális koordinációs és integrációs modellek az élelmiszer-gazdaságban” c. programja keretében valósult meg.
3 A szerző köszöni Dr. Fertő Imrének és Dr. Szabó G. Gábornak, a Magyar Tudományos Akadémia Közgazdaságtudományi Kutatóközpont tudományos főmunkatársainak a kutatás során nyújtott nagy értékű segítséget. results partly support and contradict the basic predictions of transaction cost economics. -
Product orientated sewage sludge composting technologies in the practice of AKSD Ltd.
33-36Views:538In recent years the regulations of the EU unambiguously determine that the biodegradable wastes should be used for biogas production or composting. The direct use of sewage sludge in agriculture is limited by its hazardous microelement content, but it can be used effectively in composting. The limitation parameters can be extinguished with correct compost-mixture and technology variants. The exact determination of the technologies and mixtures result a stable, constant quality product. The compost, as a classified product, is friable structured. The end-product is a
homogeneous mould with good water capacity, does not contain any weed seeds and pathogenic organisms. The compost does not have a acidify effect on the soil. It is an effective fertilizer thanks to its macro and microelement content. -
The occurrence and phenology of moth pests in different granary of Borsod-Abaúj-Zemplén County
70-75Views:324The aims of my studies were the followings: primarily to find correlation between the conditions of granary and the occurrence of moth pests. Secondly I studied the effect of disinfection on individual numbers in the population of moths. My studies were started in May 2009 in six different places of Borsod-Abaúj-Zemplén County. Indianmeal moth (Plodia interpunctella) and Mediterranean Flour Moth (Ephestia kuehniella) traps with pheromone were installed in four repetitions as well as Angoumois Grain Moth (Sitotroga cerealella) traps in also four repetitions. Control traps without attractant were also placed at every place.
From my researches, it became clear that the disinfection alone is not enough to protect cereals from moths. As in the case of crop protection, we need to apply integrated pest management.
We have to make differences between preventive protections from moth pests and the elimination of them by chemicals.
Up to my opinion, the regular checking and cleaning of the granary are important as well as the prognosis of the possible occurrence of moths. The prognosis is considered important because the studied moths do not feed at the adult stage or only at a low level. However, the caterpillars coming from the eggs placed by females can cause a significant damage in the stored cereals.
The studied sex pheromone traps are proved to be useful for the reduction of number of moths since the traps caught lots of them. These traps are relatively cheap because only the temporarily changes of pheromones increase the cost. -
Connections between the residential circumstances and the roma segregation processes in the outskirts of Hajdúböszörmény in the light of empirical examinations
187-193Views:242In addition to the processing of the available special literature, this study aims to provide an accurate view of the segregation processes in Hajdúböszörmény, as well as their effect on the real estate market by interviewing roma people living in Hajdúböszörmény and by getting to know the opinions of the affected professionals and decisionmakers working at the local authorities.
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Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:461The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations. -
Comparative analysis in residential property price level and price dynamics in urban and rural areas of Hajdú-Bihar County
85-90Views:256In modern market economies residential real estate prices, price shifts and their correlations with macroeconomic factors are surveyed quite frequently. However, in contrast with the wide scope of foreign examples, so far existing analyses in Hungary have ignored examining relation and extensity of macroeconomic indicators and failed to examine their effect on real estate pricing. The scope of this survey is to highlight these potential correlations and thus develop new aspects of analysis. Although the examination needs further extension both in time and space, the results of this survey may help to understand the importance of the responsible management of the most precious element of national wealth from the perspective of sustainable rural development.
Based on my preliminary results, there exists a strong correlation between the number of inhabitants of a settlement and the average real estate prices. Nevertheless, the correlation seems to be significant only for cities. In the case of smaller settlements the correlation still exists but at a lower level. As opposed to the results of former publications and my own expectations, no direct link could be tackled between the amount of income tax paid by private individuals and real estate prices either in the cities or in the villages within the territory and time span examined in my analysis. Although this correlation is measurable on a macro-economic level, my micro-regional analyses revealed the complexity of asset pricing and price volatility. Continuing this survey, my goal is to identify the hidden factors influencing real estate prices, whose thorough mapping may promote conscious rural development.
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The impact of grain maize and sunflower as previous crops on the yield and ear number of winter wheat in a long-term experiment
75-79Views:306We have analyzed the effect of grain maize and sunflower forecrop on three different winter wheat genotype (GK Csillag, Mv Csárdás, Mv Toldi) regarding to the spike number and yield in different fertilization levels in the 2012/2013 cropyear. According to our data after sunflower forecrop we reached higher spike number and yield, which were influenced by the differences of the varieties. We found significant differences between the varieties in the control treatment. According to our research, GK Csillag has reached the highest yield in the examined fertilization levels and forecrop models too.
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Effect of harvest on the different fertilization methods of asparagus (Asparagus officinalis L.)
33-37Views:332The experiments were performed in the University of Debrecen Centre for Agricultural Research Institute of Nyíregyháza of 1500 m2 asparagus plantation in 2011. We were determind the number of shoots, plant height, fold thickness and yield of asparagus hybrid. I studied to the effects of various cropyear is addition Grolim hybrid asparagus growth and development. Compared to previous years, 2014 was unusually wet and cold weather conditions which causes the plantation has developed outstanding. The soil temperature early and dynamic increase due to, the harvest had started a one month earlier 2014 than the 2013. The experiment asparagus harvested from lower yields as a result of the extremely dry spring in 2015.
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Review of the biology of plant psyllid (Cacopsylla pruni, Scopoli 1763), and its role in the spreading of European stone fruit yellows, ESFY-phytoplasma with Hungarian data
25-33Views:680The European stone fruit yellows (ESFY) phytoplasma disease caused by pathogen ’Ca. Phytoplasma prunorum’ induces serious damages in cherry, sour cherry, peach, and apricot orchards mostly in Europe. Its known vector is the plum psyllid (Cacopsylla pruni). Many articles report on the biology (morphology, taxonomy, life cycle etc.) and the method of transmission of the pathogen by the vector, and the possibilities of their control. This paper reviews our knowledge about the vector, and summarises the results of an inland research carried out in a northeastern Hungarian apricot orchards. Our goal was to show some important data for the farmers or anyone who is interested in this disease and its vector. And give some known method that we can protect our orchards against them to prevent the appearance of the disease. As the psyllid that became infected with the pathogen can hold its infectionous capacity during their lifetime, it is very important to have enough knowledge about their lifecycle, that we can determine the right time and method to control them. We also have to know how to identify them; therefore, this paper lists several important data which can be helpful. The most important keys of identification are their wing color, which dark borwn in the apex and brown is in the remaining part of the forewing. The length of the antennae is also an important factor, since other genuse’s species have longer antennae than twice the width of the head. C. pruni has as long antennae as twice the width of the head. They return to Prunus species in early spring and we have to protect our orhards in this period against them. We have to use preparations with a knock down effect on them to prevent the inoculation of the pathogen into the trees in our orchards.
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Harnessing diversity in durum wheat (Triticum turgidum L.) to enhance climate resilience and micronutrient concentration through genetic and agronomic biofortification
9-20Views:574Huge consumption of wheat-driven food products with low bioavailability and small concentrations of zinc is responsible for zinc-induced malnutrition and associated health complications. The contemporary durum wheat varieties have inherently tiny zinc concentrations in developing grain, which cannot meet the daily human zinc demand. Despite the fact that over two billion people are suffering from iron and zinc-induced malnutrition, various intervention measures have been deployed to reverse the effect of zinc-induced malnutrition on humans. There are evidences that agronomic and genetic biofortification approaches can increase grain yield and nutritional quality (i.e. zinc, iron, protein, and vitamins) of durum wheat to a greater extent. However, there is a lack of direct empirical evidence for which the influence of both biofortification approaches on improving human health. Application of micronutrient-containing fertilizers either in the soil or foliarly is effective in combination with NPK, organic fertilizers coupled with efficient durum wheat varieties, emphasizing the need for integrated soil fertility management (ISFM). Although genetic biofortification is a cost-effective and sustainable approach, agronomic biofortification provides an immediate and effective route to enhancing micronutrient concentrations in durum wheat grain. The application of zinc-containing fertilizers is more effective under drought conditions than in normal growing situations. Hence, this article provides a key information for agronomists and breeders about the potential of biofortification interventions to improve durum wheat yield and enrich the grain qualitative traits to ensure food and nutritional security of the ever-increasing world population.
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The Effect of Mg Foliage Fertilization on Beetroot (Beta vulgaris ssp. esculenta var. rubra) Morphology, Quality Traits and their Relations
67-72Views:333Mg-treatment increased solid soluble content in roots probably due to the larger assimilation area.
The applied foliage fertilization affected inner colour intensity and uniformity in roots positively.
The 2% Mg-sulphate solution affected pigment quantity favourably depending on varieties.
Close correlation (r=0,71) was found between the yellow (BX) and red (BC) pigments which indicates a similar biosynthetic pathway of the two compounds.
The Mg-sulphate foliage fertilization improves quality considerably but the individual reaction of varieties needs further studies. -
The Effect of Atmosperical Aridity on the Changes of Quantitative Parameters of Horticultural and Arable Crops
40-45Views:447The occurence of atmospherical drought causes serious water-supply problems in the most cases of our domestic agricultural plant species. This paper was studied, how can we quantificate the atmospherical drought, with the help of a low input (relative humidityof the air, temperature) index. If this index (LSZI) characterized the atmospherical drought well, it will suitable to estimate the yield amount of agricultural plants.
The index elaborated by the authors was tested on county average crop yield of 14 agricultural plant species. Moreover we compared the atmospherical drought index (LSZI) to other aridity parameters, how suitable for estimate the yield amount.
Result of experiments show that, the atmospherical drought index (LSZI) can be used well by several agricultural plant species in especially coern and sugar-beet to estimate yield amount. Excellent results were found by comparison to other aridity indexes, this means it is worth using in the aridity researches in the future. -
The Examination of Some Determining Elements of Efficient Practical Sweet Corn Growing
81-85Views:305We did the detailed agronomy examination and assessment of sweet corn cropping technology by analysing the data of TONAVAR Ltd. The Ltd. developed a special sowing construction which is based on band application of main sowing and double growing. In main sowing they use super sweet hybrids, and in double growing they use normal sweet varieties. In double growing sugar peas and the sweet corn can be cultivated together successfully. In every two years appearing sugar peas has a good effect on the sweet corn growing in monoculture. At the same time
the long-term successfulness of this questionable onto the illnesses of the peas because of the considerable sensitivity.
According to our examinations in main sowing the optimal period is between May 1. and 30., and in double growing the optimal period of sowing is between June 1. and 20. The optimal plant density is different too for the two sowing time. For super sweet hybrids the optimal plant number is 60-63 thousand/ha and for the normal sweet that is 65 thousand/ha.
Our examinations show that soil pest (defence with soil sterilisation in sowing time), Diabrotica virgifera, Helivoverpa armigera, Ostrinia nubialis are the greatest danger for the sweet corn quantity and quality.
The use of herbicides is the most efficient in the postemergens in main sowing and preemergens in second crop.
Our examination shows that the efficient sweet corn growing cannot be imagined without irrigation. The most efficient irrigation is in main sowing in the critical fenophase of crop time. In double growing the initial irrigation, and the crop irrigation are the most efficient. Based on the production data verifiable that beside the application of the discribed growing technology in the 2005-2007 years the average yield was 20,9t/ha of main sowing, and 17,8t/ha of second crop on chernozem soil in the Hajdúság. -
The effect of NPK fertilization and the plant density on maize yield and bioethanol production
13-18Views:471For industrial (bioethanol) production of maize, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2008.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
Investigation of the effects of biggame-preserves on forests with different species composition
21-24Views:511Hungary is well known for its high level game management. In order to develop a concentrated and more profitable gamemanagement new game preserves were established in the last few years. According to the law, in the game-preserves the game bearing capacity is not the most important parameter, because in these areas the feeding is continuous. In practice it means that in gamepreserves we find a much higher density of games than in nature. This causes a relative big loading of the area (of the herbaceous and arboreal), where the game-preserves are located.
Our aim is to study the ecological and management issues of forest- and game resources. The scene of these work is a gamepreserves related to the Nyírerdő Corporation (Nyíregyháza). We made forest structure survey to determine the main stand-forming tree species in the area of the game preserves as well as to estimate the number of the measuring points. -
Circumstances of the establishment of the Eastern Main Channel, water utilisation problems and alternatives
79-84Views:317After hundreds of years of drought in the area of Hortobágy and its surroundings brought up the idea of building a channel in Tiszántúl. It started with the idea of an irrigation canal but it ended up in a canal with high importance which can be used in many ways. Because of financial problems the necessary renovations cannot be achieved, and as a side effect the amount of silt in the water started to increase, water exploitation started to decrease, and the construction works started to get spoiled. Guided by the Water Directive the East Main Channel has become one of the most significant surface water base in Hungary. To use its water as drinking water brings up a few questions, like solving the pollution problems which problems have already occured. If we want the East Main Channel to be a solution for a long time, further steps and researches are needed to solve the problem of diverting the huge amount of precipitation of the last few years and miantaining the quality of drinking water.
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Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
23-28Views:741A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.
On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.
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The effect of collecting area on the element content of Hungarian acacia honeys
129-138Views:669Six macroelements and twelve microelements were identified in thirty-six Hungarian acacia honeys collected from ten counties by inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). One-Way ANOVA (LSD and Dunnett T3 test) and linear discriminant analysis (LDA) were used to determine the statistically verified differences among the honey samples with different geographical origin.
Significant differences were established among the samples from different counties in Na, P, S, Fe, Ni, Cu and Sr concentrations. Based on the macroelement content of honeys, the separation of samples with different geographical origin was not successful because the percent of correctly categorised cases was only 64.9%. However, examining the As, B, Ba, Cu, Fe Mn, Ni and Sr concentration, the separation of different groups was convincing since the percent of correctly classified cases was 97.2%. Thus, the examination of microelement concentration may be able to determine the geographical origin of acacia honeys.
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Effect of different lysine-metabolized energy ratio on performance of meat-type geese
39-42Views:667The aim of the experiment was to determine, how the different ratios of the digestibility lysine/ metabolized energy (0.82, 0.87, 0.91 g DLYS/MJ AMEn) of the feed influence the performance of geese. There were 150 goslings (3 treatments, 5 cages/treatments, 10 birds/cages). The experiment has started and finished at the age of 3 and 9 weeks, respectively.
The results of the experiment showed, the different ratios of DLYS/AMEn (0.82, 0.87, 0.91 g DLYS/MJ AMEn) did not influence the performance of young geese. Better growth performances were found for the 0.91 g DLYS/AMEn feed (feed intake, body weight gain, feed and energy conversion rate). This treatment coincided with high feeding costs. Based on these results we need more models and farm experiments to prove this tendency. -
Application of mycorrhizae and rhizobacteria inoculations in the cultivation of processing tomato under water shortage
111-118Views:510The effect of mycorrhizal fungi and plant growth promoting rhizobacteria on some physiological properties, yield and soluble solid content (Brix) of ‘Uno Rosso’ F1 processing tomato was studied under water scarcity. Inoculation was performed with mycorrhizal fungi (M) and rhizobacteria preparation (PH) at sowing (M1, PH1) and sowing + planting (M2, PH2). The treated and untreated plants were grown with regular irrigation (RI = ET100%), with deficit irrigation (DI = ET50%) and without irrigation (I0). In drought, the canopy temperature of plants inoculated with arbuscular mycorrhizal fungi (M1, M2) decreased significantly, however, the decrease was small in those treated with the bacterium (PH1, PH2), while the SPAD value of the leaves of plants treated only with Phylazonit increased significantly. On two occasions, inoculations (M2, PH2) significantly increased the total yield and marketable yield, however, under water deficiency, a higher rate of green yield was detected than untreated plants. In dry year using deficit irrigation, the one-time inoculation (M1, PH1) provided a more favorable Brix value, while the double treatments reduced the Brix. In moderate water scarcity, the use of mycorrhizal inoculation (M2) is preferable, while under weak water stress, the use of rhizobacteria inoculation (PH2) is more favorable.
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Evaluation of the correlation between SPAD readings and absolute chlorophyll content of maize under different nitrogen supply conditions
121-126Views:997Currently, one of the most important objectives of agriculture is to maintain the principles of the sustainability. The use of precision technologies in agriculture belongs to this topic. The use of precision technologies is increasingly widespread in the cultivation of various agricultural crops, including maize. Sensing is an important part of these techniques. In our experiment we compared two methods: measuring relative chlorophyll content and the method of determine the extractable chlorophylls. The experimental plant was maize (Zea mays L.) and the measurements were performed at an early development stage (V8) of three genotypes. Three levels of nitrogen (0; 80; 160 kg ha-1) were applied during the experiment. The relative chlorophyll content was measured by SPAD-502 (Minolta, Japan) and a handheld GreenSeeker (Trimble, USA) device. The extractable total chlorophyll content decreased in parallel with the increased nitrogen level. The obtained SPAD values were diversified furthermore the NDVI values have not been changed for the effect of different nitrogen fertilization. In the early stages of development of maize, these parameters need to be complemented with other measurements to provide reliable information about the crops nitrogen status.