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  • Effect of using antioxidants as feed additives in the diet of dairy cows on the vitamin E and lycopene content of milk
    69-72
    Views:
    144

    In 2007, the aim of an Ányos Jedlik program and call for tenders was to support application-oriented, strategic research and development projects, which can increase the competitiveness of the Hungarian economy. In the framework of our project, we intended to examine whether non-protected antioxidants – in this case: vitamin E and lycopene – used as feed-additives can increase the antioxidant content of milk. The milk with an
    increased level of vitamin E and lycopene content can be used for producing functional foods which will represent competitive products on the current market of milk products. Our results show that the use of vitamin E as feed-additive can significantly increase the amount of vitamin E in the milk. The use of lycopene as feedadditive also gave good results. At the beginning of the experiment, the lycopene content of the milk was below the detection limit, while in the post-feeding milk samples the lycopene became detectable. Based on our results, we are of the opinion that further experiments and analyses are needed regarding the quality of food of animal origin and animal health.

  • Estimation of Inheritabilities from Holstein-Friesian Test Day Data in Hungary
    23-25
    Views:
    187

    Recently, test day models (TDM) began to be increasingly used for the genetic evaluation of dairy cattle. The main advantage of the TDM compared with the 305 days lactation yield models is that more effects can be used in the evaluation. Therefore, the TDM is more accurate than the lactation models. The main disadvantage is the increased computational requirement, but this can be offset by improvements in computer capabilities.
    The topic of this paper is the use of a fix regression test day model to estimate the inheritabilities of test day data from Hungarian Holstein-Friesian dairy cattle. The inheritability was 0.26 for milk production, 0.2 for fat production, 0.24 for protein production and 0.06 for the somatic cell count.

  • Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
    105-112
    Views:
    331

    Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.

    The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.

    Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).

    This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.

  • Possibility of oil seeds in feeding dairy cows
    67-73
    Views:
    220

    The efforts to modify the fatty acid composition of milk have intensified with health conscious nutrition coming to the forefront.This experiment of ours was designed to investigate to what extent the natural-based feed additives, such as oilseeds, can influence the fatty acid composition of cow’s milk.Further information was gained about feeding of oilseeds in specific amounts to be fitted into the technology of a large-scale dairy farm in practice. The feed supplements were whole, untreated rapeseed and whole, untreated linseed, as part of a total mixed ration. In case of saturated fatty acids when supplementing with whole rapeseed the most significant change was observable in the concentration of the caprylic acid, capric acid, undecylic acid, lauric acid, myristic acid, stearic acid. In case of unsaturated fatty acids the quantity of oleic acid enhanced considerably. When observating the feeding with whole linseed the concentration of many saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, α-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers, as well.

  • Correlation between body condition change and reproduction parameters of Holstein-Frisian cows after calving
    49-52
    Views:
    129

    The authors examined the body condition with relation to reproduction of Holstein-Friesian cows on three dairy farms in the South Plain region. During the examination they analyzed the data of 516 births of 494 Holstein-Friesian cows. They were trying to find correlation between the first service, the service period and the service rates. The examinations with regard to the correlations were different from the ones published in the professional literature. The body condition at the time of calving affected the interval from calving to the first service but not the conception
    rates or the service period. The examinations did not prove any correlations between body condition changes and reproduction.

  • Study on the Work Organization at Dairy Farms
    293-296
    Views:
    74

    Joining the European Union Hungarian producers have advantages but they need to solve some problems too. Only those producers can compete who will be able to produce extra quality milk. However the circumstances of milk production are not up to the task.
    The author examined the work organization of 7 dairy farms in Hajdú-Bihar County. Data were collected about the situation of farms, equipment of barns, objets of production, work force, qualification of workers, and productivity of work. He examined the applied motivation methods at farms. He concluded that as the number of cows per farm increases work productivity increases too. From the examination it appeared that family farms applied different motivation system from bigger producer organizations. The author put forward proposals for motivation possibilities to improve milk quality, atmosphere, and general feeling of workers.

  • Production and disposition of purebred Jersey populations
    89-96
    Views:
    143

    In Hungary crossbreeding wit Jersey has been in practice since 2004. Uniquely in the Hungarian history of the Jersey breed two times fifty head pregnant heifers were imported in 2006. The aim of our study is to present through the description of the production and the type traits of the population the inherent possibilities of Jersey. The breed is presented by the figures of the rolling lactations, 305 day milk production, correlations between the traits and by the results of the evaluation of type traits. Analyses of the data were done by using independent samples t-test.
    Based on data of 87 cows the average number of days in milk  is 308 and 48 cows has finished lactation. During the rolling lactation they produced 5050 kg milk with 5.34% fat and 3.8% protein content.
    The first lactation Jersey cows’ production when corrected to 305 days was 5089 kg milk with 5.35% fat and 3.81% protein. Between the herds there was significant difference in milk kg, amount of fat produced, protein content and amount of protein produced. On the two farms distribution of cows belonging to different production levels is very different. The biggest difference is when categorized by the amount of milk produced.
    Correlation between the milk kg and fat kg is the greatest (r=0.950). Similar values were obtained for the correlation between fat and protein kg (r=0.919) and for the milk kg and fat kg (r=0,898). 
    Disposition of the Jersey cows is very well balanced. Their stature, strength, body depth, dairy form, rump angle and pin width is favorable, the average scores are between 4.5 and 6.5. The leg of the cows is a bit sickly, and is hocking in a little with medium-low angled hoof. Average values of all traits describing the udder are between 4 and 5. Cows belonging to the two herds differ by disposition. Significant difference exists in the following traits: body depth, dairy form, pin width, rear leg - side view, rear leg, rear view, foot angle, fore udder attachment, teat length. The
    Jersey population imported to Hungary has a final score of 78 points which equals with the “good” qualification. 

  • Coagulase-negative staphylococci in ewe udder surface and raw milk samples
    221-225
    Views:
    189

    Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.

  • The effect of breed and stage of lactation on the microbiological status of raw milk
    37-45
    Views:
    347

    The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.

    There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.

    Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.

  • Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
    47-50
    Views:
    206

    In the last 20–30 years lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimising lameness. Those areas are related to poor facilities, lack of straw, problems with labour and basic management.

  • Some Aspects of the Relationship Between the Quality of Sheep Milk and Dairy Products
    12-15
    Views:
    97

    The correlation between the quality of raw milk and the quality of milk products is evident. In spite of this fact we can find only a few references contained exact data related sheep milk. In recent paper we investigated the effect of SCC of sheep milk on the cheese yield (semi hard traditional cheese) and certain texture parameters of yoghurt from sheep milk. We wanted to know the relevant limit values of these properties for dairy applications.
    In our opinion – in the case of the cheese yield – that the strong negative effect can be experienced when SCC is above 700.000-1 million/cm3.
    In the case of yoghurt from sheep milk the limit values of SCC can be 1 million/cm3 for Adhesivity and Whey draining and 500.000/cm3 for Hardness. Considering these limit values in the selection of milk become materialise the highest quality of products and the economical production.

  • Effect of feeding linseed on the fatty acid composition of milk
    45-50
    Views:
    289
    In the last decades many researches were made to change the animal product food’s composition. The production of better fat-compound milk and dairy products became a goal in the name of health conscious nutrition. These researches were motivated by the non adequate milk fat’s fatty acid composition. There have been made researches in order to modify the milk’s fatty acids’ composition to reach the expectations of functional foods. With the optimal supplement of the feed can be increased the proportion of the polyunsaturated fatty acids and can decreased the saturated fatty acids. Row fat content of milk was not decreasing in the course of examination neither of the cold extruded linseed nor the whole linseed supplement as opposed to observations experienced by other authors. In case of monounsaturated and polyunsaturated fatty acids when supplementing with cold extruded linseed the most significant change was observable in the concentration of the elaidic acid, oleic acid, linoleic acid, alfa-linolenic acid, conjugated linoleic acid. In case of saturated fatty acids the quantity of palmitic acid and myristic acid lowered considerably. When observating the feeding with whole linseed the concentration of many fatty acids from the milkfat of saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, alfa-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers as well. The producing of milk with favourable fatty acid content from human health point of view can give scope propagate the products of animal origin.

     

  • Presentation of sales price reserves for live lamb
    37-45
    Views:
    101

    Although sheep breeding in Hungary is grounded in strong traditions, its activities only comprise 1% of the total production value generated from agriculture, and 2% of that for animal-based products. The most significant portion of incomes earned in the Hungarian sheep sector has, for years now, stemmed from the sale of live animals. The sector is decidedly export-oriented, as the domestic demand for its main product, i.e. lamb meat, and is minimal, equaling some 20-30dkg per person per year. Part of the sold animals is sold to market as dairy sheep, while the greater
    part is sold in the category of lamb carcasses. For this reason, the average weight of slaughter sheep has lied between 19-22 kg for years now. The target markets for live lamb sales are predominately Italy and Greece. In Greece, movement on this market has shown an upward tendency in recent years; noteworthy are also sales to Austria, Holland, Bosnia-Herzegovina and Poland. In 2003, we exported sheep meat in the form of carcasses to Italy, as well as to Germany, and of these exports, 94% went to Italy, while the remaining 6% went to Germany.
    Among sheep products, only the trade balance for live animal sales is positive. But even for such producers, only those who are specialized in intensive breeding and those sheep farms  “targeting” meat production can obtain earnings. Specialized literature on the sector argues that the quality of Hungarian lamb has diminished dramatically and is beginning to lose its market position. If Hungarian lamb does not meet market expectations, then it will only be bought from producers at lower prices.
    My research focuses on those factors which influence price. I separately discuss the question of quality, as one of the most important decisive factors on price. Within this discussion, I describe the market expectations which actually have an effect on the acceptance of live animals for sale on commission. In practice, after the problems of quality, the next most important question is that of when sale is made. In the course of my research, I studied the development of averages for sales on commission of live lambs using statistical methods. The most important problem of this sector is the hierarchical system used in sales: this is the topic hich neither the producer, nor the buyer, wish to discuss, not even with each other. On the basis of the information at my disposal, I outlined the levels of traders and individual levels used to arrive at commission prices. Finally, I examined the components of the production value of live lamb sales. The results I obtained quantified the key role of the prices and the yield, as well as the factors influencing income. 

  • Examinations connected with productive life in Hungarian Holstein Friesian populations
    32-39
    Views:
    116

    In Hungary, an intensive dairy population has developed in the last few decades as the result of upgrading to Holstein-Friesian. Intensive milk production, adjusted with housing and feeding, unfavorably affected secondary traits. The number of days between two calvings has increased remarkably and productive lifetime has decreased drastically. In the interest of profitable milk production, it is important that cows should be able to stay in the herd as long as possible, in order to have great lifetime productivity.
    The aim of this paper was to compare the production and pedigree of cows with eight or more lactations to cows which were culled after their first lactation.
    It can be stated that cows with longer herd lives produced more milk in their first lactation than those which were culled after their first lactation. When analyzing the pedigree effect of sires, this could not be proven, although some bulls, mainly of Hungarian origin, appeared at a higher frequency. In the analysis of the effect of dams, it was found that many of the offspring of the cows with longer herd lives had similarly great lifetime productivity. Correlation between herdlife and milk production quantity traits was found to be mild or stronger (r>0,60), while according to our analysis, there is no correlation between herdlife and milk production quality traits.

  • Agricultural land use and food safety
    55-60
    Views:
    222

    The world’s food production needs to be doubled in order to cover the need of the population by 2050 even if it exceeds 9 billion. The output of agriculture is expected to increase by 1.7% every year until 2020 (OECD, FAO 2011) which is a major decrease in comparison with the average 2.6% increase of the previous decade. At the same time, the meat, dairy, sugar and vegetable oil consumption is likely to increase by 2020 to a higher extent than so far. Due to the increasing food prices, the amount of starving people will increase and food consumption will decrease – especially in developing countries – as people will be able to buy less and only cheaper food products. Also, obesity may become a more severe problem and the inequality of the population’s health status could increase.

    One of the most important elements of adapting to global climate change is food safety; therefore, it is especially important to breed new biological bases and to introduce production systems which contribute to adapting to changed circumstances.

  • The trend of cowmilk yield, its compostion and the body condition during the lactation
    69-73
    Views:
    150

    The authors examined the data of 2767 trial milkings andthe months Body Condition Scores of 479 Holstein dairy cows. The condition loss was significant between the 30-60th days. The improvement of BCS begins only after the 120th day. The change in the milk protein and milk fat content were close after the condition changing. In each period of the lactation the closeness of the relationship among the condition, the milk quantity and the milk composition were different. In the aspect of the milk quantity the most significant difference was between the 2.5 and 4 BCS cows (6.68 kg) in the last period of the lactation.

  • Some strategic aspects of animal protein production
    11-19
    Views:
    199

    The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.

  • Evaluation of Origin and Conformation of Excellent Cows with Long Productive Lives
    13-17
    Views:
    110

    In the last few decades a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Beside this reproduction biology of the population was affected adversely by the keeping-, and feeding technology. Serviceable lifetime has shortened and service period has lenghtened. Whereas profitable milk production needs cows with longer productive life, larger life-time production. In our work we analysed the productivity-, and reputation parameters of the 200 highest lifetime-productivity cows of Hungary. We found that inheritance has not got important role in the serviceable lifetime. Adaptability and good constitution of Holstein-friesian is proved by the fact that cows with large life-time production are reared in large plants, some of them in large stocks, industrial environment. We found during the analysis of reputation parameters that these prominent cows mostly have deeper body, higher dairy form, higher rear udder, smaller foot angle. We found some weak connection between the milk production and the reputation. Loose correlations between useful material content and some linear traits were found.

  • Enhancement potentials for labour efficiency in small and large scale cattle farms
    70-75
    Views:
    81

    The author examined the possibilities of increasing the labour efficiency at 6 large-scale farms and at 109 small-scale farms in Hajdú-Bihar county. He stated that the level of labour efficiency was higher for large scale farms than for small-scale farms. However, there is potential for work organisation of milking, feeding and other jobs on the large-scale farms, too. The reason for the low labour efficiency on small-scale dairy farms is the small number of livestock, obsolete machinery, and lack of expertise. The author points out that the quality of raw milk is also an important issue. Using examination methods of work organisation, he determined the critical points of raw milk production.

  • The Examination of the Effects of Value Modifying Factors on Dairy Farms
    36-40
    Views:
    77

    We wish to present a method to quantify the value modifying effects when comparing animal farms. To achieve our objective, multi-variable statistical methods were needed. We used a principal component analysis to originate three separate principal components from nine variables that determine the value of farms. A cluster analysis was carried out in order to classify farms as poor, average and excellent. The question may arise as to which principal components and which variables determine this classification.
    After pointing out the significance of variables and principal components in determining the quality of farms, we analysed the relationships between principal components and market prices. Some farms did not show the expected results by the discriminant analysis, so we supposed that the third principal component plays a great role in calculating prices. To prove this supposition, we applied the logistic regression method. This method shows how great a role the principal components play in classifying farms on the basis of price categories.

  • Analysis of longevity in Holstein Friesian cattle using proteomic approaches
    21-25
    Views:
    221

    The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
    we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.

  • Microbiological status of bulk tank milk and different flavored gomolya cheeses produced by a milk producing and processing plant
    73-78
    Views:
    286

    The microbiological quality of milk is important not only for food safety, but it can also influence the quality of dairy products. In this study, our aim was to assess the microbiological status of the bulk milk of a milk-producing farm, and some natural and flavored (garlic, dill, onion) gomolya cheeses made from pasteurized milk produced by their own processing plant. We determined the number of coliform bacteria, Escherichia coli, Staphylococcus aureus, and molds of three milk and eight cheese samples. The tests were conducted between July and September, 2017.

    In bulk milk, the mean coliform count was 3.83±0.17 log10 CFU/ml; the mean E. coli count was 1.38±0.14 log10 CFU/ml; the mean mold count was 3.74±1.30 log10 CFU/ml; and the S. aureus count was <1.00 log10 CFU/ml, respectively. The mean coliform count in gomolya cheeses was 3.69±1.00 log10 CFU/g; the mean E. coli count was 2.63±0.58 log10 CFU/g; the mean S. aureus count was 3.69±1.35 log10 CFU/g and the mean mold count was 1.74±0.37 log10 CFU/g. The amount of coliforms detected in different flavored gomolya cheeses were significantly different (P<0.05). More than 10 CFU/g of E. coli was found only in the dill flavored cheeses, and S. aureus was found only in dill (3.66±1.86 log10 CFU/g) and onion (3.71±0.52 log10 CFU/g) flavored gomolya cheeses. Based on the obtained results, it was found that the amount of coliform bacteria and E. coli in bulk milk exceeded the limit set in regulation of the Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.) and the amount of S. aureus was below the limit. For gomolya cheeses, the S. aureus count exceeded the limit. The amount of coliform bacteria remained above the limit in cheeses, except for the garlic flavored gomolya cheese. In cheeses, a larger E. coli count was detected than in the bulk milk, but there is no specific limit for cheeses in the regulation. The mold count exceeded the limit specified in the regulation in cheeses, but a lower value was detected relative to milk.

    The results show that, in the case of bulk milk and gomolya cheeses, certain detected quantities exceeded the limit values set forth in regulation of MoH 4/1998 (XI. 11.). The results indicate an inadequate microbiological state of the raw material and the finished products. The reasons for these are due to reduced technological hygiene or the inappropriate handling of raw material and finished products. In this study, we have summarized the results of our preliminary studies, which can provide a basis for further hygiene studies.

  • The examination of presumed Escherichia coli count of raw milk samples on several milk production farms
    31-37
    Views:
    181

    For dairy farms, it is of great importance to insure the appropriate hygienic status of milk and to examine it regularly. Escherichia coli, belonging to the coliform bacteria type of, is a good indicator of contamination, and therefore suitable for characterising the hygienic condition of milk production.
    The aim of our research was to examine the connection between the Escherichi coli count in bulk tank milk and housing and milking technologies of different-sizes farms. We examined the relation using various statistical methods.
    Analysing the connection between the E. coli count and the farm size we found no significant difference between the farms. On the basis of the mean values of the E. coli count, we can say that the hygienic conditions are appropriate for mid-sized farms, and tolerable for large farms. We found differences in the hygienic status among the small farms. Half of the eight small farms, had no adequate hygiene. The results of the analysis of the quality categories show that the probability of inadequate quality milk was the largest on small farms (37.5%).
    Comparing the various housing and milking methods with each other, there were numerical differences in the E. coli count, but these differences were not significant. We got higher E. coli count values on those farms using tied stall barn and bucket milking installation. The reason for this could be that in cases of farms using bucket milking installation, it is harder to meet the requirements.
    After forming groups by farm size, housing and milking methods, we found that the E. coli counts are adequate on mid-size farms using various housing and milking methods; and tolerable on those large farms using loose housing stable and a milking parlour. At the same time, we found inadequate E. coli counts on the smaller farms using tied stall barns and bucket milking installation.
    The results show that if there is suitable attention, independent of farm size, housing and milking procedure, it is possible to produce milk with low E. coli counts, and to insure appropriate hygienic conditions.
    Further detailed examinations are needed to decide which factors of housing and milking technologies influence the E. coli count of bulk tank milk.

  • The possibilities of the development of functional dairy products
    33-36
    Views:
    189

    Because of our new aged world’s emerging stress, over exhausting and move less lifestyle the pollution, the not appropriate food consuming and the low calories in the consumed food there are more and more the called „civilian” disorders.
    Many people have diabetes and osteoporosis others fight with lung, cardiovascular system, problems and find cancers of many kinds without age exemptions. 
    There could be a solution in changing lifestyle. In the developed side of the world there are presence of food lines with higher content in nutritious and/or vitamins and fibers such as: kalium, calcium, selen, magnesium, plus it contains less saturated fat. Because of that there is a new word in dietetics as functional foods.
    Food having more inner content and/or biological values, so healthier, are called functional foods. Those foods components picked carefully for healthiness by modern knowledge of dietetics.
    One of the best raw materials for functional food is the milk. It is already healthy by itself. The Körös-Maros Biofarm Ltd. has a goal of developing and marketing, health protecting organic functional foods in hungaricum products from cured milk. The Jedlik Ányos project helps the Ltd. to achieve this goal. We just finished an antioxidant test and we check if the antioxidant in present in the milk than we check the final product of the presence of the original antioxidant and the form and amount of it.
    It had been feeding, for two weeks for three herd of cows selected for age, milk output and consanguinity. All of the three herd had been feeding with basic forage. One of the herds had been getting vitamin-E in the amount of 250 mg/bwkg the other group got licopin in the amount of 200 mg/bwkg once a day each by each orally, the third herd was the control group. 
    After two weeks we toke a sample of every herd were processed the samples into yogurt and cheese. Than we checked the raw milk, the cheese and the yogurt for antioxidant content with HPLC method. The test ended with good results by finding a great amount of antioxidant, in not only the raw milk but also in the final product.