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Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
105-111Views:203The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. -
Seedling morphology of different wheat genotypes at early stages under hydrocultural conditions
249-254Views:413Consuming “sprouted seeds” is one of the most important factors of a healthy diet. An experiment was conducted in the University of Debrecen, Research Centre of Nyíregyháza (Hungary) in 2014 to analyse some morphological traits of four winter wheat genotypes (Triticum aestivum L.) and one spelt (T. spelta) variety. Our results showed that the spelt wheat variety “Franckenkorn” could maintain higher root length throughout the experimental period. On average, both “Perbetei” and “Franckenkorn” varieties could maintain higher root number compared to the other varieties. The extensive breeding line “1401 HK” had the highest shoot length throughout the whole experiment, being significantly higher than the landrace variety “Perbetei” and both of the varieties “KG Bendegúz” and “KG Kunhalom”. It could be concluded that “KG Bendegúz” cultivar and “Perbetei” landrace seem to be the most suitable for aquaculture techniques. In addition, “1401 HK” breeding line can be the most suitable for the production of juice since the minimal required shoot length (12 cm) was achieved within the shortest period of time after sowing (9 days). This breeding line and “Franckenkorn” can also be suitable for production of “wheatgrass”, because it is consumed without roots. Further research is needed to evaluate nutritional values of these genotypes.
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Development of a new measurement method to determine plant antioxidant status
105-112Views:330The antioxidant capacity of 12 cultivar that were harvested in 2014, were determined by FRAP (Ferric Reducing Ability of Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity) TEAC (Trolox Equivalent Antioxidant Capacity) and photochemiluminescence method. In sour cherry, the most antioxidant effects of natural bioactive compounds are anthocyanins. Our results show that the photochemiluminescence method is the most suitable to determine the antioxidant capacity of red soft fruits and tart cherries
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Does drought stress always negatively affect the yield and quality of soybean in Hungary?
37-40Views:392Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.
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Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
100-104Views:282Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection. -
Storage of wheat at high moisture
111-114Views:153Deterioration rates were determined for 15-19% moisture content wheat (Dropia cultivar) stored at constant temperatures.
Deterioration rates were determined by measuring germination capacity of the grain and respiration rates of grain. Safe storage
time was defined as the time for germination to decrease to 90%. Safe storage times of 19% m.c. wheat stored at constant
temperatures ranged from 2.5 d at 30 and 35°C to 37 d at 10°C. Deterioration rates of 19% m.c. wheat stored with a step decrease
in storage temperatures (35-25, 30-20, 25-20, and 20-15°C) were determined and safe storage times were satisfactorily predicted.
Safe storage times of 17% m.c. wheat were 5, 7, and 15 d at 35, 30, and 25°C, respectively. Respiration rates and germination
percentages of 15 and 16% m.c. wheat stored at 25°C remained constant for 70 d. The respiration rates of 17-19% m.c. wheat at
25°C increased while the germination percentages decreased with storage time. Germination dropped from 98 to 92-89% when the
dry matter losses were about 0.05% and visible mould occurred when the dry matter losses were about 0.1% in 17-19% m.c.
wheat. -
Examination of the usability of the MALDI-TOF method in sunflower genetic identity analyses
33-38Views:308Economically, one of our most important crop is the sunflower. Regarding its production it is essential to use top quality seeds. As the conditions of seed certification and the certification parameters are regulated by laws and ordinances, it is highly important to farmers and seed producers to detect seeds of low quality or dubious origins.
Nowadays, the examination of the cultivar homogenity of sunflower is based on a reference method of the International Seed Testing Association Rules International (ISTA), Chapter 8. This standard reference method uses ultrathin poliakrilamid isoelectric focusing gel electrophoresis (IEF-UTL, or simply IEF). With this work we have set out to compare the results of MALDI-TOF to the test results – used as reference results – achieved by this reference method. The aim is to develop a new, quick, cheap and reliable method. In this article I summarized the results of some of the experiments that will provide basis for my further work.
During our previous experiments we have concluded that out of four extracting agents we can get the most protein markers using NaCl acid buffer, 1 propanol buffer. -
Environmentally-benign plant protection possibilities against domestic Monilinia spp. in organic apple and stone fruit orchards
101-105Views:386In this study, possibilities of environmental-friendly plant protection against domestical brwon rot species were summarized for oecological pome and stone fruit orchards. Symtomps of the two most important brown rot species (Monilinia fructigena (Aderh. & Ruhl.) Honey and Monilinia laxa (Aderh. & Ruhl.) Honey) were described and then cultivar susceptibility to brown rot was discussed. Furthermore, mechanical, agrotecnical, biological, and other control possibilities (stone powders, plant extracts and restricted chemical materials) were shown.
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Comparative study of newly-bred black locust clones with regard to photosynthetic rate and water use efficiency: early evaluation
5-10Views:492Black locust (Robinia pseudoacacia L.) is one of the most important tree species in Hungary, due to its positive economic impacts. Research to increase its yield, improve its stem quality and enhance its drought tolerance has been ongoing since the 1960s. Of the current research works in this field, the clone trial of the Forest Research Institute, University of Sopron, established in 2020 in the Nyírség region, is worth highlighting. In this experiment 4 newly-bred clones and a state-approved black locust cultivar ('Üllői') are being tested. In the summer of 2022, ‘on site’ measurements of assimilation parameters – net assimilation (An), transpiration (Tr) – were carried out using the LI-6800 portable photosynthesis system. From the data obtained, the water use efficiency (WUE) was calculated. The results of the statistical analysis (Kruskal-Wallis H test) have shown significant differences (p < 0.05) between the clones for all three parameters (An, Tr, WUE) tested. The NK2 clone has performed the highest value for all the parameters studied. However, no significant differences were found between clones NK2 and PL040 for Tr or between NK2 and control ('Üllői') for WUE. Studies of this kind contribute to the improvement of black locust growing through the production and selection of cultivars, which are relatively resistant to the negative effects (drought) of climate change.
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The effect of different shading net on the Quantum Efficiency of PS II in chilli pepper cultivar ‘Star Flame’
21-23Views:533The study was undertaken to identify the effect of different shading net on the quantum efficiency of PS II on ‘Star Flame’ chilli pepper (Capsicum annuum) over a period of time cultivated under plastic house environment. ‘Star Flame’ pepper was grown under red shading net and samples without shading were used as control. Analysis of photosynthetic activity revealed a significant difference (p<0.05) between Fv/Fm values in control and red shading at the end of July (p = 0.031) after the first harvest. Chlorophyll fluorescence parameter Fv/Fm reflects the maximum quantum efficiency of photosystem II (PS II) used in the detection of early stress in plants.
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The effect of climatic change on the rheological properties of winter wheat doughs
96-100Views:255In present paper we have examined the effect of climatic change on the extensigraph characteristics of wheat-flours. The baking quality
of winter wheat is largely determined by cultivar, but it can be influenced by weather conditions during growing period. Flours were from 5
cultivars grown at one location in three cropping years. We have found that the extensigraph properties of dough are affected by the weather
conditions, nevertheless, different cultivars distinctly react to the increase of temperature and decrease of precipitation. In generally, the
higher average day temperature and lower precipitation level is favourable to produce winter wheat flour with better quality -
Fruit quality of sweet cherry cultivars in superintensive orchards
75-81Views:654The fruit quality of 15 sweet cherry cultivars (’Canada Giant’, ’Celeste’, ’Chelan’, ’Ferrovia’, ’Germersdorfi Rigle’, ’Katalin’, ’Karina’, ’Kordia’, ’Linda’, ’Regina’, ’Sam’, ’Sandra Rose’, ’Sunburst’, ’Sylvia’ and ’Techlovan’) was studied under super-intensive growing conditions at Nagykutas. We measured the fruit diameter, fruit width, fruit height, stem length and stem weight, fruit and pit weight and the total dry matter content. There were large differences among the cultivars. These differences are due to the genetic characteristics of fruits because all other conditions were the same. For 11 cultivars, we collected fruit samples several times /2-4/. We examined on this cultivars all the above listed fruit quality parameters. When examining these samples, we have gained information how earlier or later than optimal harvest time influences fruit quality.
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Screening of paprika (Capsicum annuum L.) varieties resistant to NaCl salt stress
105-110Views:360Salinity stress is one of the environmental factors that negatively affect the growth and production of pepper plants. The 100 seeds' weight was measured. The total fresh weight of five seedlings and the growth rate of one seedling of three paprika varieties were also measured under the influence of NaCl at a salinity level threshold of (3 dS m‑1). The proportion of tissue water content in three pepper cultivars was measured according to a mathematical formula at the end of the experiment. In terms of seed weight, the (Carma) cultivar outperformed other types greatly. Except for the superiority of both (Carma, and Bobita F1) over (Fokusz) variety in total fresh seedlings weight under sodium chloride as abiotic stress, there are no significant differences in the total seedlings' dry weight and the rate of seedling growth. The non-drought-resistant type (Bobita F1) loses water the fastest, at 89.61%, compared to drought-tolerant kinds, which lose water at a slower rate (Carma, and Fokusz). The results demonstrate the (Carma) variety's numerical vigor, particularly in the growth rate. More testing is needed to determine the selection of varieties that are resistant to abiotic and biotic stresses.
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Comparative Senses-based Investigation of Traditional and Modern Apple Cultivars
70-74Views:130Apple consumption has significantly decreased over the last 10-15 years in Hungary. While consumption of fresh apple was 25 kg/person/year before the change of regime, today it hardly exceeds 10 kg according to careful estimations. In spite of the fact that the former dull cultivar structure (Jonathan, Starking, Golden Delicious) has widened with numerous new cultivars, apple consumption in Hungary has not increased. Unfortunately, customers do not or scarcely know most of these new cultivars.
In a survey conducted among the students of five Hungarian universities, we gathered information about comsumer opinion of both and older cultivars.
Numerous questonnaires – 592 – were filled out on 8 cultivars (Jonagold, Novajo, Topaz, Golden Reinders, Idared, Pinova, Braeburn, Jonathan, Starking) about the qualitiy parameters that determine the value for consumption. The results clearly show that besides the old cultivars, such cultivars are preferred that are known worldwide, such as Jonagold, or Golden (free of russetting) or the resistant Topaz.
Promotion of these cultivars and creating the possibility of their supply can help to increase a fresh apple consumption. -
Examination of extreme water-balance of maize cultivar in different crop rotation systems in 2007
33-40Views:187We examined the change of the time of water balance of soil in long-term experiment, on chernozem soil, in different croprotation systems (mono-, bi- and triculture). We found the smallest difference between the water deficit of not irrigated and irrigated plots in triculture. We concluded that irrigation impressed favourably on water balance of soil in both of crop-rotation systems. Water deficit has decreased significantly after irrigation
in 25. May in mono- and triculture. Irrigation moderated only values of water deficit. Irrigation in 30. June not influenced water balance of soil in both of crop-rotation because of a big drought. Water deficit of soil lessed till harvestperiod because of rainy season at the end of August and in September. -
The impact of cultivar and irrigation on yield, leaf surface temperature and SPAD readings of chili pepper
103-108Views:443Paprika is an extremely popular and widespread plant species in Hungary. Chili peppers belongs to paprika and it can be consumed fresh or processed. The production of peppers began with the cultivation and control of some parameters for good results of paprika production. The aim of this article is to compare the water supply of four chili pepper hybrids ("Habanero", "Hetényi Parázs", "Unikal" and "Unijol") by means of investigation on open field and to examine how different temperatures and water stress factors influence the yield of chili peppers. The experiment area consisted of four different hybrids in three different irrigation treatments (control, deficit- and regular irrigation). The chlorophyll content was estimated with SPAD and the leaf surface temperature with Raytek MX4 TD. Our research concluded that deficit irrigation produced the highest yields for all cultivars and the lowest yields for the highest water supply.
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The effect of the dates of shoot selection and shoot tip removal on the growth of William’s pear trees
65-70Views:208Increasing the intensity of production in the case of pear can be the solution to satisfy the market demand forr high-quality fruit. The aims in this technology are the canopy treatments and the maintainance of consistently high quality yields in the long run. The experiments were performed with cv. William’s pear, a cultivar grown on large areas in Hungary. To create the optimal canopy shape for earlier fruiting, we performed shoot selection and the cutting back of shoot tips. Our results show, that both pruning methods – applied at 3 different dates – decreased the number of short fruiting parts (spurs), and increased the number of vegetative shoots. The sole exception was from this the effect of shoot tip removal when done at the earliest date.