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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:119Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:92Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Examination of microsatellite markers of Dorper sheep breed
57-61Views:239Number of not woolly and molty sheep exceeds 60 million throughout the world. Their numbers and their importance is growing, still they have appeared in the past two decades all over in North-America, Australia, New-Zealand and also in Europe. The South African Dorper has been a pioneer among them in Hungary. It was introduced in 2006 in the country. The Dorper sheep is the second largest breed in South Africa, which was developed from the crossing of Dorset Horn and the Blackhead Persian. The aim of the EU Member States in terms of this specific breed is increasing the small populations, improving the productive qualities, in addition to this avoiding inbreeding. However, finding appropriate breeding stock is difficult due to the small size of available populations and also to the suspected common of origin. With the help of various molecular genetic methods we could get a total view of the genetic background of these flocks. Nowadays the most commonly known and used genetic markers are microsatellites, because their applications give fast, accurate and easily reproducible results. There is no specific descriptive information on the genetic background of Dorper populations in the various EU countries , also regarding diversity between populations. Therefore in our work we want to optimize the conditions of applicability of 31 selected microsatellite reactions as a first step of mapping the entire genetic background of the different EU Dorper populations.
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Awareness and adoption of a nurse sow management system among small-scale pig farmers in Nakuru County, Kenya
113-120Views:166This study aimed to assess the level of awareness and adoption of nurse sow management strategies among small-scale pig farmers in Nakuru County, Kenya. The research included a survey of pig farmers who visited the Nakuru Agricultural Show in July 2023. A total of 139 farmers were interviewed within 5 days of the show. The corresponding author interviewed the respondents in a face-to-face engagement, where questions on nurse sow management were initially drafted in English and translated into Kiswahili, i.e. the second language in Kenya. The obtained results indicated that farmers came from two main regions of the county: the northern part (54.7%, 76/139) and the southern part (45.3%, 63/139). Levels of awareness and adoption were insignificant between the two groups (χ2, p<0.05). A major obstacle to the adoption of this strategy was identified as feed challenge at 77.7%; (108/139) and market issue at 59.7%; (83/139). This study identified important aspects and limitations that should be considered when developing sustainable productivity development strategies for Kenyan pig farmers. To assist the sustainable growth of small-scale pig production, the authors suggest government measures that shield farmers against exploitation of feed and marketing components, as well as advocating for effective breeding to increase live born.
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Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
105-111Views:123The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. -
Anaerobe degradation of maize infected by Fusarium graminearum
57-61Views:200Last year intense rainfalls and moisture conditions were beneficial for the Fusarium sp. in Hungary. Fusarium strains decrease cereal quality (for example maize), furthermore may cause yield loss. Due to the toxin production, the fungi have a dangerous animal and human pathogen effect (Placinta et al., 1999).The effects of the Fusarium infection and its mycotoxin production haven’t been perfectly eliminated. Fusariumgraminearum
is the most common agricultural pathogen in Hungary. The utilization of infected maize as an alternative biogas raw material may be an efficient and environmentally friendly disposal method. In this case, Fusarium-, and mycotoxin-content of the maize have to be analyzed as well as the impact of these factors’ on the biogas production process. Our experience was based on the raw material basis of a biogas plant. Different amount of Fusarium free and infected maize grits have been added to the regular raw material mixture. The detection of Fusarium fungi has been analyzed
in experimental digesters throughout the different stages of mesophilic digestion. In the biogas liquid end product the Fusarium was detected by breeding and by microscope. According to our results, the Fusarium sp. was not detectable in the liquid end product after 30 days. -
Examination of the effects of the cropyear and the nutrient supply on the quality of winter wheat with the help of Győri’s Z-index
121-125Views:72In the case of winter wheat,the knowledge of several quality features is needed to be able to determine precisely the real quality of the given
wheat. Several systems have been worked out on the qualification of the winter wheat in Hungary and other countries as well. Evaluating the
quality is being made more difficult because the different quality features take part in the development of the quality in different degrees and
the values of the several quality features are in different intervals and these data are different dimension values. On the evidence of the
results, in the case of considering several features,it can be difficult to rank into one concrete quality cathegory. Researchers are trying to
develop complex quality index numbers in order to be able to define the quality more precisely. One of these complex quality index numbers
is Gyıri’s, so called, Z-index.
In three years from 2006 and 2008, we examined the change of the quality features of nine varieties of winter wheat with the help of the
Z-index under the influence of the effects of the different cropyears and the fertilizer treatment. The results show that the Z-index of the
examined varieties of winter wheat were influenced by several factors. Examining the data of the three different breeding years together we
can observe the corrective effect of the different cropyears on the Z-index, and if we examine the three years separately and together, the
differences of the quality features of the different varieties differentiate very well, and with the help of the Z-index the comparison of the
types is easier and perspicuous, and the Z-index represents the different nutrient reactions of the different types as well. On the average of
the three years, the types gave the best results at N120-150+PK nutrient level. Among the nine varieties the best results were given by Mv Suba,
GK Békés and Mv Mazurka in the case of both low and higher nutrient levels. -
Increase of Wheat (Triticum aestivum L.) Resistance to Leaf Rust (Puccinia tritici) via Gene Transformation
127-129Views:86Leaf rust is one of the most significant fungal disease of wheat not only in Hungary but also in other parts of the world. For improving leaf rust resistance of winter wheat variety (Hajdúság, 2003) produced by conventional breeding methods, verified by results of variety tests, showing outstanding results in the aspect of the most important economic values, integration of tissue culture technics, genetic engineering and traditional
methods may provide facilities. Building the gene(s) responsible for resistance into the determined genome can improve the resistance in a way that changes other features of the plant slightly or not at all. In the course of genetical transformation of the variety Hajdúság we applied one of the wheat’s own effecient green-tissue specific insurer genetical regulator, the promoter of ribulose carboxylase 1-5 bisphosphate (RuBisCo) ‘s small
subunit to control the expression of the gene cmg1. -
The Developmental Possibilities for Methods of Real Estate and Farm Property Evaluation in Agriculture
241-247Views:97Different methods for evaluating property have gained greater importance in agriculture since the change of regime. The open market evaluation could be the best method if the agricultural property had significant turnover, which could serve as a reliable comparison. However, there is no notable turnover, and selling is scarce. And, when there is some, the sales contracts are not available. On the other hand, the open market evaluation is almost the only one assessing method in Western Europe and in the United States. The matrix comparing market data in my methodological development helps to estimate the effects of the elements which determinate real estate value of farms.
Another method for evaluating farm property is the discounted future earnings. Enterprises are able to produce series of income continuously during their working periods, which can be considered as perpetuity. This is the base of the discounted future earning evaluation. Determination of income generating capacity is not an easy task. It is also difficult to choose the proper rate of capitalization. If this rate is higher than the usual level, the property will be underestimated. If it is lower, the property will be overrated. According to my calculations the profitability of certain farms of animal breeding may be evaluated under different operating conditions. Furthermore, the capitalisation interest rate may be determined in an objective way.
The problem of applying the depreciated replacement cost evaluation method is that there are big deviations among investment costs in tenders of contractors; moreover the estimations of special depreciation forms are rather subjective. One of the process’s greatest difficulties for reliability is that it is doubtful whether depreciated replacement cost could reach the real market value. The utilisation of this method may be spread further in the future by standardising average gross replacement cost of building as well as by measuring depreciation more objectively. -
Studying the reproduction-biological effect of the GPR54 receptor gene in sheep
48-51Views:149One of the main economical problems of sheep breeding is that continual production is not possible due to the seasonality of animals. Today, genes, mutations that may develop aseasonality in sheep are extensively researched in order to make the establishment of populations capable of aseasonal estrus possible.
According to the current knowledge, the GPR54 gene participates in the GnRH release from the hypothalamus, and thus in the alteration of the reproduction state of the organism. Our research is aimed to determine whether the GPR54 gene has allele variations that influence the proneness to aseasonal estrus in sheep in a different extent. Therefore, the GPR54 gene sequence of sheep is first examined. -
The effect of different fat content fish feed on the production parameters and meat quality of the hybrid striped bass
33-37Views:209The interspecific ’hybrid striped bass’ is the result of cross breeding. Their offspring have got good chances of growth and tolerate extreme water temperatures and the content of dissolute oxygen more than their parents. Furthermore, they are more resistant to diseases. Therefore, the hybrid is perfectly suitable for intensive fishery production.
Our experiment lasted for seven weeks. We analysed the indices of growth and production of the hybrid as the consequence of nurturing them with two feeds of high nutritive value during this period. We examined the content of fat and of fatty acids in the flesh of these fish. There were no considerable differences in terms of weight gain, feed conversion ratio and uneven growth between the two groups, while survival was less likely among the the fish which had consumed more fat. We demonstrated, examining the flesh of the individuals which had consumed feed containing lower fat content, that it was fattier and contained more dry matter than those that had been fed of higher fat content. Besides, the content of omega-3 and omega-6 fatty acids was higher in the fish that had consumed less lipids.
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Selection of Sport- and Racehorses
3-10Views:121The utilisation of the horse has changed from time to time in response to human needs. For a few decades, it has been serving in several equestrian sports more intensively. It has also been proved that the standards for this kind of performance cannot be established in the way certain characteristics, such as the weight gain or milk production of other animal breeds can. Breeding horses for sporting comprises highly complex selection criteria.
Some of these (e.g. external features, temperament, manageability and intelligence) do not put the breeder in a difficult position, but finding the traits that establishes the safety of sporting achievements poses a genetic problem.
The performance of a horse for sports is a highly complex feature, which cannot easily be assessed or put down in figures. In addition, man plays a decisive role in shaping all kinds of performance of a horse at any given time by not only creating conditions for a better performance, but also by playing an active role in increasing it.
The performance of the horse is mostly defined by its general aptitude to movement, ie., the regularity, clear rhythm and springiness of basic types of strides, as well as the ability to move in a naturally balanced way. Training and riding principles are based on these traits. These two together will determine about 70% of the value of the horse and its adequacy for high performance equestrian sports. In order to avoid subjectivity in determining the above variables and to increase the degree of objectivity, competent expert teams (trainers, judges, other riders) are employed to form an opinion on an individual animal.
Assessing horse performance outside races does not seem to be efficient, as owing to the dominant effects of the environment, the indicator of inheritability is hardly above 0.1.
Free jumping is an especially appropriate means for assessing a horse’s readiness and ability to move in an environment free of disturbing factors. In free jumping, it is especially important to judge the style of the jump. The first phase of jumping – as a sequence of movements – lasts from the moment the fore-feet touch the ground until the moment the hind-feet push off, while the second phase lasts from this moment until touching the ground. The most important task in the first phase is to make the angle of the dip of the body by the supporting fore-feet that is necessary for the jump. The quality of the jump is determined by the jumping and adequately expanded hind-legs. The intensity of pushing off and jumping done by the hind-legs can be inferred, and differences between individuals can be discerned from the shaping of the curve by the hocks and the paths of the pasterns in relation to the withers. -
Optimizing fish structure in angling ponds focusing on white fish
33-36Views:188According to new amendments of laws, commercial fishing licenses of natural waters shall not be issued from the calendar year 2016 in Hungary. However, the most settlement of white fish to angler ponds originated from natural waters. Currently, there is no sufficient quantity of fish species belonging to this group available to fulfil the settlement commitments of fish farmers. The aim of this recent study is to develop a breeding and production technology that is suitable for settlement of large quantities of this fish species of appropriate length of (4–5 cm). The following methods are investigated: aquaculture cage system (pond-in-pond system), monoculture and mixed population with different ratio of white fish in fish structure. Due to the different environmental needs of different fish species, many parameters need to be investigated, for example: stocking density, feeding, oxygen level, monoculture or mixed populations, growth potential and mortality. A preliminary experiment was carried out following the artificial propagation of ide (Leuciscus idus) following a 21-day larval rearing experiment in which the effect of different stoking density was investigated for mortality and growth. On the basis of the expected results, the optimal conditions for the aquaculture cage system will be described, regarding to the white fish stock.
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Large Scale Measurement of the Total Antioxidant Capacity of Different hungaricum Fruit and Vegetable Samples with Photochem Chemiluminometric Device
300-304Views:120In our present study, we performed a quick test of the antioxidant capacity of domestic fruit and vegetable cultivars with a PHOTOCHEM® instrument (Analytik Jena AG, Germany). The instrument is special because it can measure both the total lipid-soluble antioxidant capacity (ACL method) and the total water-soluble antioxidant capacity. Another advantage of the system is its quick measurement. With similar instruments, such measurement can take several hours, while PHOTOCHEM® can perform it within a few minutes.
In our model studies, we examined the changes in the antioxidant capacity of sour cherry, green pepper, carrot, pumpkin, red beet and Brassica sp. cultivars. We aimed to determine how suitable the instrument is for quick, routine measurements in the case of the different horticultural products and which species have high antioxidant capacity values.
On the basis of our examinations, we can state that the PHOTOCHEM® system is suitable for routine, semi-automated measurements of the total antioxidant capacity at several vegetables and fruits. Besides this, the system can be used for testing the quality of food products, for monitoring storability in post-harvest technologies, and for special breeding programmes and for physiological and biotechnological studies. -
Evaluation of the Green bean variety candidate BU-16
75-81Views:88This study presents the results of the variety comparison trials conducted with the French bean variety candidate BU-16 on sandy soil, during two years at the Research Center of the University of Debrecen.
The outstanding characteristics of this variety candidate are the early maturity, the showy pods (yellow, straight, cylindrical cross-section „pencil bean”) and the high yield potential.
The experiments were conducted with a randomized design, with five varieties and four replications. The varieties (of which two are foreign and three are domestic) were as follows: BU-16 fj, Maxidor, Paridor, Hungold and Budai piaci. The individual plots were 2 m wide and 4 m long. The seeds were sown at a density of 30 germs per m2, with a row spacing of 50 cm.
The following parameters were taken: time of flowering, time of green maturity, plant height, height of the lowest pod above ground level, number of pods per plant, distribution of the pods by the state of development (20 plants), usable pod length (1 kg of standard crop-yield), yield per hectare based on the number of plants per plot. The experiments were evaluated statistically with Excel and SPSS softwares.
Results obtained with the variety candidate BU-16:
– The time from the emergence to green maturity is 45 to 46 days, which is short as compared with the other cultivars studied.
– Plant height is 38cm (two years average), which is among the highest ones, together with Paridor.
– The pods are set high above the ground level similarly to Paridor, the height of the lowest pod is 19 cm.
– The average number of pods per plant is 13 (two years average) similarly to Paridor and to Maxidor.
– The distribution of pod size in 2007 is as follows: 68% of the pods of BU-16 is of the standars size, which is a good rate. The value of this trait for the other varieties is 60 to 63%, except for Paridor, the value of which is similar to that of BU-16.
– The usable pod length (10 to 14 cm) is 63% in 2007 and 84% in 2006. Similar pod length rates were obtained for Maxidor, while the rate for Paridor is as high as 91%.
– The total yield and the standard yield of BU-16 is among the highest ones.
According to the results obtained and presented above, the variety candidate BU-16 is the earliest maturing among the varieties tested with high yield potential which, however, is not significantly different from that of the others. Significant differences can only be detected in plant height and the number of pods per plant. Considering the results, BU-16 is to be further tested. -
Examination of resistance to Sclerotinia stalk and head rot in sunflower (Helianthus annuus L.) hybrids
34-37Views:162Nowadays, phytopathogenic fungi cause the most serious yield loss in open field cultures, and sunflower (Helianthus annuus) is no exception to this phenomenon. Sclerotinia stalk and head rot (Sclerotinia sclerotiorum) is present in the whole area of Hungary, and can cause serious financial loss. In our experiment, sunflower hybrids were tested for resistance to Sclerotinia sclerotiorum infection. 16 sunflower hybrids were examined at the Experimental Breeding Site in Jászboldogháza. Pesticide treatment and also nutrient replacement were applied on the sunflower fields.
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Effect of year and irrigation on yield and quantity of different potato varieties
7-14Views:135dramatically in the last few decades. We are lagging behind Western European countries as regards yields. The ecological conditions are not favourable for potato, but the low yields other causes: the use of non-suitable seed, the low level of irrigation and nutrient supply and out of date machinery. The competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habit, and often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and selected quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2004 and 2005, on 50 m2 parcels on calcareous chernozem soil after winter wheat (2004) and two rowed barley (2005) as a forecrop. The varieties were examined in 4 repetitions in randomized blocks, of which two repetitions were irrigated, and two were non-irrigated. We examined the yields of the varieties, the distribution of tubers according to size and their percentages and changes, and selected parameters of quality and chemical composition due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However, the impact of irrigation depends on the year effect. -
Water relations composition among Egyptian cotton genotypes under water deficit
5-15Views:206Background: water shortage is one of the major factor effects on growth characters and yield of most crops. Objective: this study was conducted to get to know the reactions of some Egyptian cotton genotypes to water deficit. Methods: The genetic materials used in this study included thirteen cotton genotypes belonging to Gossypium barbadense L., from the Cotton Research Institute (CRI), which was devoted to establishing the experimental materials for this investigation. Results: the ratio of GCA/SCA was less than unity for all studied indices, indicating predominance of non-additive gene action (dominance and epistasis), which is an important in exploitation of heterosis through hybrid breeding. Results: The data showed significant reduction in water relationship characters for all parental genotypes under stress conditions. The Egyptian variety Giza 68 gave high values for most water relationship characters. Data revealed that the greater the value of tolerance index is, the larger the yield reduction is under water deficit conditions and the higher the stress sensitivity is becoming. The parental genotypes Giza 96 showed the highest reduction in yield under water deficit conditions. At the same time, the cross combination Minufy x Australy showed higher values of yield reduction followed by the combinations Giza 67 x Australy. Of the male parents, the Russian genotype 10229 recorded the best GCA values for most water relationship characters. At the same time, the female parents, the old Egyptian genotype Giza 67 recorded the best values and exhibited good general combined for most water relationship characters. The cross combinations Giza 86 x Pima S6, Giza 77 x Pima S6, Giza 94 x Dandra and Giza 96 x Australy showed significant desirable SCA effect for most characters. Conclusion: relative water content %, osmotic pressure, chlorophyll and carotenoids content indicates better availability of water in the cell, which increases the photosynthetic rate. Also, the higher level of proline accumulation in the leaves which was recorded under deficit water suggests that the production of proline is probably a common response of plant under water deficit conditions.
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The Effect of Drought on the Yield of Winter Barley Lines in the Great Cumania Region
127-129Views:81Winter barley is the third most important fodder plant in Hungary after winter wheat and maize.
Its accommodation to the changing climatic conditions – mainly to drought in Great Cumania, the driest region of our country is of great importance from the point of view of plant breeding and growing.
The vegetation period of 2002/2003 can be considered to have been very droughty with 247.5 mm precipitation.
According to our results six- rowed barley lines have better drought tolerance than two-rowed lines. The average yields of the six-rowed barley lines were 12-25 per cent higher than the yields of the two rowed lines.
Due to the joint effect of delayed sowing and drought, significant yield depression was detected both in the case of the early and the medium maturity group, and assessable data could be gained only from 35 per cent of the total territory. -
Survival Analysis of Hungarian Large White, Duroc and Pietrain Sows
31-36Views:286The aim of the present study was to perform lifetime performance analysis in three pig breeds; Hungarian Large White (n=295), Duroc (n=76) and Pietrain (n=91) on a commercial farm using analysis of survival sows. We took into consideration the age of sows at the time of their inclusion into breeding, their age at the time of culling, time spent in production, number of mating and parities, parity percentage, intervals between litters, number and mean of piglets born alive and born dead, number of raised piglet litters, number and mean of 21 days old piglets, the weight and mean of raised litter and raise percentage.
We carried out the analysis by SPSS 22.0. Single factor analysis of variants, Kaplan-Meier analysis and Cox PH model were used. The determination of the significance of risk rates differences was done by Wald chi square test.
Our results showed that the average culling age were 1056 (±33.52) days for the Hungarian Large White, 735 (±73.56) days for Duroc and 818 (±71.98) days for the Pietrain.
The log rank test of the survival analysis indicated a significant difference between the three tested genotypes (χ2=16.981, P<0.001), which means that the survival percentage of the individual breeds varied significantly from one another. In comparison with the Hungarian Large White genotype the Duroc genotype has a 1.6 times higher (P<0.001) culling risk while that of the genotype Pietrain was 1.36 times higher (P<0.001).
Our results can be used to compare the breeds kept under the same conditions and to compare the life span of one genotype under different farming conditions. Factors that increase survival and improve the profitability of pig farming can be determined by this method.
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Agrobotanical Analysis of Bean (Phaseolus vulgaris L.) Equilibrium Populations Suitable for Regional Land Cultivation
148-152Views:73The local bean landraces maintained at small-scale farming level should have importance as complementary source for completion of plant genetic resources with potential role in local land cultivation. Also, they should be used as primary material in breeding activities targeted on improving the foodstuffs quality. In the research were used especially landraces collected in Nyírség, Körös-mellék and Tápióvidék region. Our main objective was to reveal the relationship between the adaptation ability of landraces and the distance from their original collecting place. For that reason the location of the experimental plots was choosen by taking into account that they must be checked in each region, on it’s original region and similarly in other two different regions. On the base of the recorded agro-morphological traits we can get answers on the shaping of adaptation ability, and in the same time by a general chemical characterisation we should know more about the variation of qualitative parameters with a potential economical role. In the designing of experiment a main importance had the conditions where the research is taking place. All activities related to the maintenance of experiment were done in frame of a low input agricultural system to emphasize as much as possible the natural endowments of regeneration places.
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Morphological diversity of current melons (Cucumis melo) compared to a medieval type
84-90Views:168Morphological diversity of melon (Cucumis melo); phenotype reconstruction of a medieval sample. Morphological diversity among 47 melon (Cucumis melo) cultivars and landraces from Hungarian germplasm collection (ABI, Tápiószele) were analyzed with an ultimate aim to characterize morphologically cv. Hógolyó, which showed the closest genetic similarity to a medieval melon recovered from the 15th century. Cultivars based on fruit morphology were grouped into the three main types of melon as reticulatus, cantalupensis and inodorus. Cluster analysis (by SPSS-11) based on 23 morphological (quantitative and qualitative) traits recorded revealed an extreme diversity among accessions, nevertheless cultivars were clustered into main melon clusters with only two exceptions of inodorus type cv. Zimovka J. and Afghanistan. Cultivars Sweet ananas and Ezüst ananász; and two Hungarian landraces Kisteleki and Nagycserkeszi showed close similarity. Cultivars Hógolyó and Túrkeve of inodorus type
were also grouped in one cluster, which provide insight into the morphological reconstruction of the medieval melon recovered from the 15th century. These results also indicate that old Hungarian landraces could be re-introduced into breeding programs for broadening genetic base of melon. -
Relationships of Fruit Production and Regional Development in the Northern Great Plain Region
181-187Views:116The role of retaining population in agriculture is stronger and more significant in the long run in the North Great Plain Region compared to other regions. The region has a significant processing industry along with a good basis for producing raw materials, developed food processing capicity and high quality agricultural products typical of the region. The GDP in agriculture, forestry and game management is somewhat higher than the national average.
Variety is of cardinal importance when establishing the quality of horticultural products and determining the product value. The Hungarian breeding results of apple, quince, apricot, cherry, raspberry, red and black currant are promising.
The regulation system of EU the vegetable and fruit market is based on Retail Cooperatives Producers. With the establishment of national vegetable- and fruit production and retail organizations, the market regulation, production and quality development issues of the sector can be handled and solved. Reaching EU standards in fruit production can only be achieved with up-to-date plantation systems and breed selection. A key issue in development is establishing the required financial resources for investments and updating production. In order to reach these standards, significant state subsidies and good credit conditions are needed. By solving these tasks, the sector is expected to become self-financed without government help.
The pursuit of safer production, improving quality and increasing yields require the establishment of up-to-date irrigation systems. The improvement of family farms, motivating land concentration is necessary for increasing average size of plants. Establishing the above mentioned conditions is important since the vegetable-fruit sector is of great significance in the employment of rurally based population, improving their living conditions and executing rural development programs. -
Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:334Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:124We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.