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DHAC-induced transgene overexpression in 35S-gshI GMO poplar (Populus × canescens)
78-83Views:129Relative gene expression levels of transgene gshI (γ-glutamylcysteine synthetase cloned from E. coli) were analyzed by qRT-PCR in two transgenic poplar (Populus × canescens) clones (11ggs and 6lgl) and wild type (WT). An extremely high expression level of transgene gshI was observed in the 6lgl clone (13.5-fold) compared to 11ggs (1.0) samples, which level was doubled (1.8-fold) in the DHAC (5.6-dihydro-5'-azacytidine hydrochloride) treated (at 10-4 M for 7 days) 6lgl samples but not in the 11ggs clone (0.4-fold). Contrary to this result, relative copy number of transgene gshI in the 6lgl clone was found to be less 60% less (1.0) then in the 11ggs samples (1.6). Relative expression levels of proper poplar gene gsh1-poplar showed significantly higher responsiveness to DHAC treatment than transgene gshI with the highest expression level in the untransformed (WT) poplar
clone (19.7-fold) compared to transformed 6lgl (8.7-fold) and 11ggs (2.5-fold) clones. For internal controls constitutively expressed housekeeping genes a-tubulin were applied. For data analysis, the 2−ΔΔCt method was used. DHAC applied in long-term cultures (27 days) at low concentrations (10-8 – 10-6 M) showed morphogenetic activity by initiating de novo root development of leaf discs. -
Statistical comparison of soil analysing results of chernozem soils
93-99Views:355The soil fertility was degraded as a result of unreasonable tillage, therefore the application of site-specific nutrient replacement is necessary. It is essential for the application of precision fertilization to know the location, extension, soil properties and nutrient-supply of the different soil types of
cultivated areas.
We collected soil samples from 580 hectares of land in 2006. Soil samples were collected from every 5 ha in 30 and 60 cm depths during Spring from 20.05.2006 to 12.06.2006 and again in Autumn from 09.19.2006 to 02.10.2006. Soil samples were analysed at the Department of Agricultural Chemistry and Soil Science of DE-ATC.
The two examined soils are slightly calcareous, weakly saline, poor in zinc. The calcareous chernozem soil is slightly acid, the content of humus, nitrogen, phosphorus and potassium is medium in this soil. The meadow chernozem soil is slightly alkaline, and properly supplied with humus and potassium, and middling supplied with nitrogen and phosphorus. The meadow chernozem soil is more heterogenous in soil plasticity, lime, saline, nitrogen phosphorus and potassium content and less heterogenous in pH and zinc content than the calcareous chernozem soil.
Standard deviation of measured values in pH, soil plasticity, humus and nitrogen content significantly differ between the examined soil types. The soil plasticity, pH, humus, nitrogen and zinc content significantly differ among calcareous chernozem soil and meadow chernozem soil, but the difference in phosphorus content can be statistically proven only in case of Spring soil sampling. -
Opportunities of utilization of real-time ultrasound technique in beef cattle breeding
45-52Views:364The goal of the authors was to evaluate the repeatability and accuracy of real-time ultrasound technique under Hungarian conditions. Ribeye area was measured at first time on live animals, before the day of slaughtering with Falco 100 ultrasound equipment in Charolais fattening bulls. Ultrasound pictures were traced at two times by the same operator. After slaughtering, ribeye area (REA) was measured on the carcass by planimeter at the same anatomical point like ultrasound measurements. Statistical analysis was carried out to examine the relationship between carcass and ultrasound REA. After it, repeatabilty was established by comparing the ultrasound pictures twice.
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Agronomic bases of precision barley production
217-220Views:220To ascertain the importance of individual preciosion factors in achieving yield and quality of malting barley, we established a multifactor
experiment at the research station of the Slovak University of Agriculture in Nitra in 2009 and 2010. Four variants of fertilization, 2 ways of soil cultivation and four varieties 'Bojos’, 'Kangoo’, 'Marthe’ and 'Xanadu’ were observed. From the quality indicators the nitrogen content (%) was observed. The results were statistically analyzed by using a multifactor analysis of variance by using Statistica 8, the program Statgraphics. The difference between years was statistically significant (1.87 t ha-1) in favour of 2010. The difference was also significant between the varieties 'Bojos’ and ’Kangoo’(1.07 t ha-1), respectively. 'Martha’ and 'Xanadu’ as well as 'Kangoo’ (0.56 resp. 0.33 t ha-1). Conventional soil cultivation in
comparison with a minimalization technology, demonstrated a tatistically insignificant increase of yield. Significant differences were obtained between the variants of fertilization. Treatment by using Condit (b-var.) showed a very positive impact in climatic favorable year (2010) with a yield result of 7.42 t ha-1. In comparison with an untreated control, the difference in yield was 0.89 t ha-1. A significant increase of yield was achieved by using the combination of solid fertilizer with foliar fertilizer (LAV + Hakofyt, var.c) 0.47 t ha-1. The crude protein content was statistically influenced by a variety and year. In 2009, the crude protein content was above average (12.38%). Significantly lower attributes were achieved in 2010 (9.90%). From the studied/observed varieties the lowest crude protein content was showed by a variety 'Kangoo’ (1.68%). -
Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:807The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Effects of the development of production factors on productivity
13-18Views:253In every economic sector, the enacted and expected quality of the products strongly affects its market entry and subsistence. The aim of management is success, competitiveness and subsistence that require adequate human and material resources and enough production stock. We must also notice that the quality of tools and instruments significantly influences work productivity. This applies to both plant production and animal husbandry.
The author researched milk production factors with methodical observation, document analysis and interviews. Data were collected with a ten-year interval, thus this assay can highlight the field, volume and sources of investments during this period and that the production stock increased in most farms and decreased in few. The data also shows the influence of investments on work productivity - there are differences between farms of equal size, so there are still ways of development.
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The effect of foreign stallions on the Hungarian Furioso-North Star breed
67-70Views:569The most common aim of animal conservation programs is to maintain genetic diversity. Furioso-North Star is an indigenous Hungarian horse breed originated from Mezőhegyes Stud. The breed is based on two founder stallions, Furioso Senior and North Star Senior. The aim of this research study was to analyze the effect of the foreign breeding stallions to the genetic structure of Hungarian Furioso-North Star breed. The genetic structure of the breed was studied from pedigree data what was received from the Furioso-North Star Horse Breeding Association. Foals born between 2015–2018 were chosen as reference population. Every breeding stallion was marked by nationality (Austrian, Czech, German, Hungarian, Romanian or Slovak) according to their birth place. The population was described with genetic variability, what was calculated using Endog software. The Furioso-North Star breed is popular in Central Europe and nearby countries. The stallion imports and the stallion transfers were necessary and useful as they made changes in the composition of the genetic variability. The new genes and the new lines have refreshed the genetic structure. There were several breeds, like the Nonius, Shagya Arabian and English Thoroughbred, whose had an impact on the genetic structure of the Furioso-North Star breed.
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Effect of the Cropping Year on the Quality of Winter Wheat
89-95Views:528We examined the formation of quality parameters of winter wheat in a small plot variety comparison experiment from four cropping years. Our aim was to estimate the year effect on several quality parameters of winter wheat.
We established in relation to the distribution of precipitation of the examined years that the years 1997 and 2000 were behind the thirty year average typical precipitation of this site both in the case of the whole vegetation period and the spring and summer. It was unfavourable for qualitative wheat production. 1998 and 1999 were good for cropping considering the amount and distribution of rainfall.
Examining the formation of quality parameters we found that the baking value was maximum in 1997, with a moderate amount of normal distributed rainfall for the examined varieties. The mid-late maturating varieties showed better baking value in drought years than in wet years. In the case of the wet gluten content, we established higher values after a rainier spring-summer period. There is a conspicuous difference between the falling number of extensive and intensive varieties and the year had significant effect on the formation of values. We established with correlation analysis that both the precipitation of vegetation period and the maturing rainfall had considerable effect on the value of falling number. Examining the effect of fertilization on the formation of wet gluten content as a quadratic equation, we found that precipitation may both increase and maximalize the value of this quality parameter. -
Classification of a diffuse heavy metal polluted mining site using a spectral angle mapper
119-123Views:851Characterization of heavy metal polluted abandoned mining sites is complicated, as the spatial distribution of pollutants often changes dramatically.
In our study, a hyperspectral data analysis of the Gyöngyösoroszi abandoned Pb-Zn mine, located in northern Hungary, where Záray (1991) reported serious heavy metal contamination, was carried out using ENVI 4.3. In this area, galena (PbS), goethite (FeO(OH)), jarosite (KFe3(SO4)2(OH)6), sphalerite ((Zn, Fe)S) and pyrite (FeS2) were the predominant minerals in the alteration zones was chosen as the target mineral.
Spectral angle mapper (SAM) and BandMax classification techniques were applied to obtain rule mineral images. Each pixel in these rule images represents the similarity between the corresponding pixels in the hyperspectral image to a reference spectrum.
As a result of hyperspectral imagery the distribution of pyritic minerals (sphalerite, galena) in the area was defined. Both of the mineral formations occur, especially in mine tailings, the area of the ore preparatory, and the Szárazvölgyi flotation sludge reservoir. According to the results, jarosite and goethite have similar distributions to sphalerite and galena. The results showed that hyperspectral remote sensing is an effective tool for the
characterization of Pb, Zn and Fe containing minerals at the examined polluted sites and for modelling the distribution of heavy metals and minerals in extensive areas.
This classification method is a basis of further detailed investigations, based on field measurements, to map the heavy metal distribution of the studied area and to quantify the environmental risks caused by erosion, which include DEM (digital elevation model) and climatic and hydrological data sources. Furthermore, it can be used primarily to support the potentially applicable phytostabilization technique and to isolate hot spots where only ex-situ remediation techniques can be applied. -
The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:357he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Analysis of the situation of domestic game damage and forage ground management
5-8Views:364Game damage is an important problem in home wildlife management, because game managers pay more and more money for game damage year by year. Crop fields can do an appreciable part in reduction of game damages. The aim of the research is to survey the situation of domestic game damage and wildlife forage ground management. (Which are the most used plants on crop fields, how current are the using of seed mixtures, which are the most typical game damages, as well as which are the most applied game control methods?)
It can be stated that the number of plant species sown on crop fields is quite low. Mostly corn and alfalfa are raised, though besides those many other plants are suitable to grow on crop fields. Rising value of game damage and big proportion of the agricultural game damage are well traceable from the questionnaires, too.
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Methane emission from Matsuo rice paddy field in light of different fertilizers, costs, profit and carbon credit
9-13Views:283Nowadays global warming is a major issue to our environment. This issue is generated by the modern human activities like industry and intensive agriculture. This research is about methane emission from rice paddy fields. The aim of the study is to lower the methane emission from the field with the help of using different type of fertilizers, whilst we keep in focus the efficient economic operation. The main experimental field is Matsuo paddy field, (Matsuo town, Sanbu city, Chiba prefecture) which is analyzed by the Chiba University’s soil science laboratory, they provided the data for this study. During the study three type of fertilizer was analyzed which are all organic and the control was a regular chemical fertilizer. For all fertilizers the cost and income of the production were calculated and the profit was weighted with the methane emission what a specific fertilizer produced during the cultivation. In the future if the organic fertilizers are in focus than it is necessary to find a new material what can be competitive with the chemical fertilizers in focus of GHG emission or find an alternative way of the usage of methane in biogas production.
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Phenotypic characterization of Satu Mare Tumbler pigeon breed
31-34Views:335The aim of this study was to determine morphological characteristics of the tumbler pigeon breed of Satu Mare. This pigeon breed was first described in 1932 and was very close to extinction at the end of the 20th century. Data collection was carried out in two stocks having birds from three breeders (Bertalan Juhos, Cosmin Lupu and Werner Hartmann) in Satu Mare. In addition to the breeder, sex and color variety were also evaluated. The performed conformation measurements were statistically compared using multifactor analysis of variance. Lupu’s birds were longer compared to other breeder’s stock, while beak and wing was longer in Juhos’s stock. Thoracic perimeter was smaller whereas queue length was the longer for Juhos’s birds. Cocks’ values were significantly exceeding values of hens, except beak length. Recessive red pigeons had longer body than blue birds. Beak length of recessive yellow pigeons was significantly longer that it was computed for black birds. Wingspan was longer for dun birds than it was estimated for recessive yellow pigeons. These differences show that there is reasonable genetic variability in the breed which is desired in small populations. Due to the variability within the breed, stabilising selection might be favorable, with a special account for avoiding inbreeding.
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Búzaminták fungicid szermaradvány ellenőrzése és gombafertőzöttségének kimutatása
45-51Views:165To protect crops with chemicals against different microorganisms are very important because residues of toxins could pollute infected grains. This could have the meaning of serious danger to the human and animal health. Similarly important is knowledge of details about presence or absence of any practically applied protective compounds. Experimental studies were performed on fungicide treated cereals of the Cereal Research Non-Profit Company, Szeged. Chromatographic methods were used (GC and some GC-MS) to find low level fungicide residues, microwave-assisted isolation of ergosterol was followed by HPLC to detect possible fungal infections and a more detailed microbiological analysis completed the work. Concerning the dry year 2000, no fungicides were found in the samples (by November), the ergosterol level was found to be less than 8 ppm, meaning good quality, coupled with an extremely low level of fungal infections. This was confirmed by direct microbiological testing. In comparison with the microbiological results from 1999, a negative difference can be recognized, most probably due to the rainy weather throughout the year.
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Evaluation of Soil Degradation Based on High Resolution Remote Sensing Data
145-148Views:374Soil salinity is the main problem of soil degradation in the Grate Plain with cultivated area of 20% affected. Its influence is accelerated on the water managed and irrigated lands. Remote sensing can significantly contribute to detecting temporal changes of salt-related surface features. We have chosen a farm where intensive crop cultivation takes place as a test site as soil degradation can be intensive as a result of land use and irrigation. In order to evaluate soil salt content and biomass analysis, we gathered detailed data from an 100x250 m area. We analyzed the salinity property of the samples. In our research we used a TETRACAM ADC multispectral camera to take high resolution images (0,2-0,5 m) of low altitude (300-500 m). A Normalized Vegetation Index was computed from near infrared (750-950 nm) and red (620-750 nm) bands. This data was compared with the samples of investigated area. Analyzing the images, we evaluated image reliability, and the connection between the bands and the soil properties (pH, salt content). A strong correlation observed between NDVI and soil salinity (EC) makes the multispectral images suitable for construction of salinity map. A further strong correlation was determined between NDVI and yield.
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Study of plant production modells with different intensity in winter wheat production
51-60Views:419The effects of crop rotation, nutrient supply and plant protection technologies were examined on the yield of Mv PÁLMA winter wheat variety and on the most important diseases of ear and leaf. Our experiments were carried out on chernozem soil in the Hajdúság in 2006 and 2007, and three plant protection technologies (extensive, average, intensive) and three irrigation variations (without irrigation, irrigated with 50 mm, irrigated with
100 mm) were applied in different crop rotation systems.
In the triculture crop rotation a higher rate of infection was observed than in the biculture crop rotation, because the vegetative growth was more expressed after pea and these microclimatic factors were favourable for the development of pathogens.
In the triculture crop rotation (pea – wheat – maize) the powdery mildew, DTR and leaf rust of wheat were present in both examined years (powdery mildew 5-15%, DTR 14-42%, leaf rust 8-37% in cropyear 2005/2006, powdery mildew 12-32%, DTR 9-29%, leaf rust 8-26% in cropyear 2006/2007). Fusaria could be observed in 2006 (depending on the plant protection technologies and nutrient supply in the biculture 7-27% and in the
triculture 5-19%). With higher amounts of fertilizers the rate of infection increased and reached its maximum at the highest dose of nutrient supply (N200 +PK).
We observed the highest rate of infection by ear and leaf diseases in the case of the extensive technology, while this rate could be considerably reduced by the application of the intensive technology.
Both in 2006 and 2007, yields were the highest at the N100-200+PK levels in the triculture after pea (6028-7939 kg ha-1 in cropyear 2006, 6578-8690 kg ha-1 in cropyear 2007 depending on plant protection technologies), and at the N150-200+PK levels in the biculture after maize (6096-7653 kg ha-1 in cropyear 2006, 4974-8123 kg ha-1 in cropyear 2007 depending on the plant protection technologies). The highest yield maximums were
reached when pea was the forecrop. The yields on the experimental plots of the intensive plant protection technology was 224-2198 kg ha-1 higher (depending on the forecrop) compared to the plots where the extensive technology was used.
The highest yield without irrigation was at the N150+PK both in biculture and triculture crop rotation. Among the irrigated variations Ö2 and Ö3 at N200+PK fertilisation resulted in the highest yield in the biculture crop rotation, while the N100+PK level in triculture system. In the biculture crop rotation the extra yield was 14-51% higher (575-1225 kg ha-1 depending on plant protection technology) when 50 mm water was irrigated, and
15-54% higher (778-2480 kg ha-1) if 100 mm irrigation was applied comparing to the non-irrigated versions. The yield was 7-17% higher (560-1086 kg ha-1) in the Ö2 irrigation variation, and 8-23% (691-1446 kg ha-1) higher in the Ö3 irrigation variation compared to Ö1 irrigation variation (non-irrigated).
A correlation analysis was made to reveal the connection between the yield, the amount of fertilizers, the rate of infection, the plant protection technologies and the forecrops. Strong positive correlation (0.846) was found between year and fusaria infection. Strong positive correlation was observed between fertilization and powdery mildew infection (0.525), fertilization and DTR (0.528), fertilization and yield quantity (0.683). Lower
correlation was found between fertilization and leaf rust infection (0.409), and forecrop and yield (0.472), recpectively. Negative correlation was calcutated between plant protection technologies and DTR (-0.611), and plant protection technologies and leaf rust (-0.649). -
Examination of extensographical parameters of winter wheat (Triticum aestivum) flour
109-115Views:453In the trade of the European Union principally the analysis of alveographical and extensographical parameters mean the acceptance system.
In the present study we analysed the extensographical parameters of 10 winter wheat varieties breed by the Cereal Research Non Profit Company with Brabender extensigraph and we made a comparison, correlation among the results. The examinations with Brabender the following among: show the GK Kalász and the GK Élet varieties show high resistance of extension and the GK Petur variety produces high extensibility. The GK Garaboly has shown low extensibility and energy. The other parameters had different values in the examined three years. The measurings with SMS2 Texture Analyser show middle positive correlation between extensibility and subarea. There are weak correlation between at the significantial level in the 90, 135 resistence of extension by Brabender and by SMS2 Texture Analyser and in the 45 extensibility by Brabender and by SMS2 Texture Analyser of relaxed dough. There is no correlation among other parameters. -
Analysis of vitamin E content in pilot dairy products
31-34Views:402Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount. -
Some context of the multifunctional agricultural and sustainable rural development
29-37Views:281The paper first negotiates the concept of multifunctionality, then the main steps of the development of the connection between agriculture and rural development are discussed. It presents the situation of a most disadvantageous sub-region that is aided by a complex program. The local rural resources are estimated by the multifunctional rural resource analysis method considering threefold function of the rural areas. It is concluded, that the land use systems and labour-intensive cropping systems have specific missions in multifunctional agriculture and rural development in the sub-region.
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Brief overview of the polymorphism analysis of genes affecting pig prolificacy (LEP, PRLP, ESR BF, EGF, FSH-β, H2A.Z)
5-10Views:458Researches are being performed around the world to increase swine prolificacy by using marker-assisted selection (MAS). The present study processes researches of polymorphism examinations on 7 genes. The result of the experiments showed that the leptin gene (LEP) prolactin receptor gene (PRLP), estrogen receptor gene (ESR), properdin B (BF) epidermal growth factor (EGF), follicle-stimulating beta gene (FSH-ß) and Z member of the H2A histon family gene (H2A.Z) and their alleles have a positive effect on reproductive characteristics of different swine breeds. In addition to this, leptin gene (LEP) influences the build, meat production and growth of body fat. Further studies are concerned with the polymorphism of an increasing number of genes, which enables a faster genetic development of swine breeding.
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The scientific background of competitive maize production
33-46Views:1052The effect and interaction of crop production factors on maize yield has been examined for nearly 40 years at the Látókép Experiment Site of the University of Debrecen in a long-term field experiment that is unique and acknowledged in Europe. The research aim is to evaluate the effect of fertilisation, tillage, genotype, sowing, plant density, crop protection and irrigation. The analysis of the database of the examined period makes it possible to evaluate maize yield, as well as the effect of crop production factors and crop year, as well as the interaction between these factors.
Based on the different tillage methods, it can be concluded that autumn ploughing provides the highest yield, but its effect significantly differed in irrigated and non-irrigated treatments. The periodical application of strip tillage is justified in areas with favourable soil conditions and free from compated layers (e.g. strip – strip – ploughing – loosening). Under conditions prone to drought, but especially in several consecutive years, a plant density of 70–80 thousand crops per hectare should be used in the case of favourable precipitation supply, but 60 thousand crops per hectare should not be exceeded in dry crop years. The yield increasing effect of fertilisation is significant both under non-irrigated and irrigated conditions, but it is much more moderate in the non-irrigated treatment.
Selecting the optimum sowing date is of key importance from the aspect of maize yield, especially in dry crop years. Irrigation is not enough in itself without intensive nutrient management, since it may lead to yield decrease.
The results of research, development and innovation, which are based on the performed long-term field experiment, contribute to the production technological methods which provide an opportunity to use sowing seeds, fertilisers and pesticides in a regionally tailored and differentiated way, adapted to the specific needs of the given plot, as well as to plan each operation and to implement precision maize production.
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Tourism-Based Analysis and Development Potentials in Hortobágy
272-278Views:241Tourism – especially ecotourism and health-tourism – could be one of the sectors which could make Hungary economically competitive after joining the European Union, by ensuring thousands of people employment possibilities, if they take advantage of local and regional opportunities. For this reason, it would be necessary to analyse the two types of tourism mentioned above, as well as to collect data and especially impressions concerning how these potentially lucrative sectors could best be structured and managed, as well as to predict their probable effects.
The most important objectives of my study are to introduce the values of Hortobágy, to analyse the present status of tourism in the Hortobágy National Park, and to make suggestions for increasing the numbers of tourists, by making Hortobágy more attractive.
As a resident of Debrecen, I visited the region several times and took part in programs at Hortobágy as well. I was always curious how the trip to the Hortobágy could be made more enjoyable.
My research was carried out with the help of questionnaires, which were made in three languages – Hungarian, English and German –. In August 2002, I approached foreign and Hungarian tourists in Hortobágy village, and asked them to fill them in. To evaluate the questionnaires, I used Microsoft Excel ’00. During the evaluation, I calculated distribution, arithmetic mean and deviation. Moreover, I obtained the level of significance.
My assumption that tourists visiting Hortobágy are “one-day” tourists, who are visiting the Hungarian Pusta because of an actual program, was supported by my research. I must emphasise that mostly passive tourism opportunities are offered in Hortobágy, where tourist are only external observers of the programs.
In order to change this situation, I suggested that visitors to Hortobágy can be encouraged to stay longer by offering them various programs, in which tourists are actively involved. My program recommendations include a craftsman’s house or tent, where the visitors can try to make typical folk instruments. Moreover, I made suggestions as to how to teach tourists to make Hungarian dishes. I also outlined several one-day program possibilities. Among the opportunities, I also mentioned the need to provide tourists possibilities to spend several days in a conventional Pusta life-style. To realise these things adequate infrastructure and information system should be developed. -
Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
112-118Views:533The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method with a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction. -
Comparison of the alveographic qualification and the baking quality of winter wheat
294-302Views:666On the basis of the operative French regulations, we qualified the alveographic qualification and the valorigraphic qualification respectively for each of the 10-14 winter wheat varieties grown each year between 2002-2005, and used in a field experiment at the Karcag Research Institute of the University of Debrecen. The field experiment at the Látókép Research Institute of the University of Debrecen allowed us to examine the alveographic and valorigraphic values of the samples of GK Öthalom and Fatima 2 winter wheat varieties taken from an 8 year period (1997-1999 and 2001-2005).
Throughout the experiments on samples from the Karcag Research Institute and Látókép Research Institute of the University of Debrecen, we tried to find whether the alveographic W value or the P/L value provides the safer method for the qualification of wheat samples, instead of the qualification commonly used in Hungary. We found that in terms of the samples from the Karcag Research Institute, the baking quality and the W value in each of the examined years, while in terms of the Látókép Research Institute, most of the years showed a close positive correlation, contrary to the close negative relationship between the baking quality and the P/L value. The correlation index varied depending on the year, the variety, and fertilizer doses, which require further examinations. -
Analysis of small ruminants’ semen under the cooling, deep-freezing and sex-orientation method
53-58Views:251The aim of this study was to examine the influence of cooling, deep-freezing and sex-orientation methods on fertility of ram and buck semen. It was pointed out that deep-freezing and sexorientation methods had a more considerable destruction on both semen compared with the effect of cooling method. However, with the development of the sex-orientation method, the results of lambing had a significant increase in sheep. On the other hand, the NRR of the inseminations with deep-frozen ram semen exceeded most of previously publicated results. Being interesting, and hopefully useful benefit in practice that the analysed buck semen samples are shown more favourable results in all methods compared with the same results of rams.