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Compost application in integrated and organic fruit cultivation
135-139Views:118Nowadays the success vegetable and fruit production are unimaginable without regular nutrient management. One of the ways to supply the required nutrients in an environmentally friendly way is the application of composts, which is less widespread so far. Compost doses were applied in biological and integrated apple orchards in cooperation with the Institute of Horticultural Science in the years 2010 and 2011. Different changes were resulted by the compost treatments in the examined parameters in case of both apple varieties (Golden Delicious and Pinova). There is no clear effect of compost on the changes of ash-, total acids, Vitamin C and the sugar content until now, because the improvement of the nutritional indicators takes more time. However significant differences were observed after one year between the apple varieties. Higher ash-, total acids and sugar content were measured in case of the apple variety Pinova, while the measurements showed higher Vitamin C content in case of the variety Golden Delicious.
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Near infrared spectroscopy (NIT) analysis of maize hybrids starch content
119-122Views:144The purpose of my research is to find the most favourable maize hybrids for bioethanol-production feedstock, using the so called „NIT”, near infrared spectroscopy method in the analysis of the inner contents especially of starch content. The other goal of my analysis is to reflect on how the effects of the most important growing factors – such as irrigation and the use of different dose fertilizers – contribute to the maize hybrids starch content.
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The Effect of Sowing Time and Nutrient Supply on the Yield Stability of Maize
75-80Views:126Sowing time, nutrient supply and plant number play crucial roles in the yield stability of maize. The productivity of various hybrids, each with its own genetic characteristics, was tested for three different sowing times and five different fertilizer doses. The highest yields were achieved at the third sowing time (17. V.), which is unusual, because the second half of the summer was rainy and was favourable for late sowing. The seed moisture content at harvest was higher than the optimal 14-15% at the third sowing time, the hybrids, which have intensive bleeding dynamics, couldn’t reach the lower seed moisture content at harvest of the early sowing. In that case we have to decision whether the plus yield of the third sowing time cover the drying costs.
Some hybrids produced the highest yields by N 120, P2O5 75, K2O 90 kg/ha active agent but the higher fertilizer doses depress the yield. The other part of the hybrids were able to produce high yield by bigger fertilizer doses. On the whole the agro-ecological optimum of the NPK fertilization was N 120-160, P 25-100, K 90-120 kg/ha active agent, but the N 80, P2O5 50, K2O 60 kg/ha fertilizer doses was the most effective. -
Changing of antioxidant and sugar contents of self brewed beers during the brewing steps
161-166Views:178Beer is a valuable beverage, its composition is influenced by raw materials and technology. We tried to determine how the technological steps influence the antioxidant content, the colur value, and the carbohydrate content, and how fruit syrups influence the antioxidant content and the colour value.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:215Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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The impact of environmental factors on the measurement of the normalized difference vegetation index
141-147Views:136The level of nitrogen supply of a plant population can be quickly measured with non-destructive optical measurement devices and the differentiated determination of nitrogen shortage and the replenishment of nitrogen can also be carried out. The level of nitrogen supply is based on the fact that the chlorophyll content of crops is in close correlation with nitrogen content and that the amount of chlorophyll can be easily measured on the basis of the light absorption of chlorophyll molecules. The successfulness of optical measurements can be influenced by the change of weather parameters; therefore, it is important to know the correlations between measurement results and weather parameters when it comes to practical use.
The GreenSeeker Model 505 measurement device determines the relative chlorophyll content in the form of the Normalized Difference
Vegetation Index (NDVI) calculated on the basis of the intensity of the reflected red and infrared rays of light from the crop population. The measurements were performed in alfalfa population with 10 replications at five measurement heights and four measurement times. The weather parameters were measured by a weather station located in the middle of the alfalfa population and the correlations between the meteorological data and the NDVI values were examined. During the statistical evaluation of the results, it was established that the NDVI measurement is primarily influenced by the relative humidity of the air, secondly by air temperature and thirdly by wind speed. Relative humidity was in strong correlation with the NDVI values which were also influenced by the measurement height and time. Regression was not significant in the case of 20 cm measurement height, but the measurements above 40 cm height showed significant correlations. The correlation was shown to be strong at each measurement time, but the influence of humidity was the lowest at 11:00 and 14:00. -
Variation of boza nutrition parameters based on raw materials
67-70Views:161Boza is a ceral based fermented drink. Raw material of boza could be rise, millet, wheat and rye. Because of the diversity of raw materilas and the method of fermentation boza might show varied quality and nutrition value.
Nowadays healthy and conscious nutrition are playing more and more important roles in our lives. People pay an ever growing attention to the nutrition value of products, such as protein- and sugar content, components with antioxidant properties, and the mineral content of the products. In our research we wanted to know how the protein,-, sugar-, total polifenol-, mikro- and macro element content of products change.
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Examination of the effect of pelleted poultry manure products on a sunflower test plant in a laboratory model experiment
83-88Views:205Sunflower (Helianthus annuus L.) is one of the most important oil plants in Hungary, with a sown area of more than 627.000 ha in 2019. Sunflower cultivation is primarily important for use in the oil industry, but its role in feed and beekeeping cannot be neglected either. Sunflower adapts well to different production areas in terms of soil demand, yet, as important fact, it must be grown on soils with poorer conditions with intensive nutrient replenishment. From the agri-environmental point of view, in addition to the use of pelleted poultry manure products, which are also new to the consumer market, is of paramount importance. Their advantages include a wide range of nutrients as well as a soil structure improving effect.
In our research, we investigated the starter effect of pelleted poultry manure products applied at different doses (200 kg ha-1 and 400 kg ha-1) on sandy soil with humus using a sunflower plant. The aim of our research to investigate the effect of using pelleted fertilizer products as starter fertilizers on sunflower plant germination, and investigate the effect of poultry manure products on soils.
Based on our results, it can be stated that the pigment content (chlorophyll and carotenoid content) was positively influenced by the higher dose treatments (400 kg ha-1), thus increasing the photosynthetically active leaf area. Compared to the control, the carotenoid content in the test plants of the treatments increased, and the differences of this treatment proved to be significant (p<0.05). Our experiments supported the beneficial effect of pelleted poultry manure-based products in the soil-plant system.
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Effect of harvesting time on the vitamin C content and yield of chili pepper
101-104Views:122For thousands of years, chili pepper has been used in cooking intensifying the aroma, colouring, and even flavouring with its pungency. Today ,it is also of interest for the various food and pharmaceutical industries due to its intrinsic characteristics. These characteristics of pepper and its yield can be affected by both the variety and the temperature fluctuation throughout the harvest period. The current work's objective was to investigate the effect of harvest time on vitamin C of different varieties of chili pepper C. frutescens and C. annuum cultivated in Hungary, as well as the yield of pepper in different harvesting periods. Vitamin C was determined by HPLC protocols. The differences between harvest times and varieties were analysed using Tukey post hoc test at 5%. All varieties showed fluctuation of vitamin C level, depending on the given harvest date. At the last harvest, all varieties had higher vitamin C content. All varieties had the same yield trend throughout the year but with different yields between each. Variety Hetényi Parázs had the highest vitamin C content and yield: 3720.53 µg g-1 vitamin C and 18.63 t ha-1 average yield, respectively.
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Using sensors in precision crop production
267-270Views:127Proper plant nutrition that takes into consideration both the requirements of plants and ecological conditions is one of the most important precondition of successful plant production. An important element of the N-fertilization of wheat is that the optimum zone of nitrogen supply is significantly narrower than that of other plant species, therefore it can easily happen that we apply higher or lower nitrogen doses than the optimal one. A possible solution to this problem can be precision agriculture. Applying the methods of precision agriculture we can take into consideration the heterogeneity of fields. By applying precision methods either online or offline we can intervene faster than if we would rely only on regular soil and plant analysis procedures. The determination of the doses of nitrogen and the timing of application are influenced also environmental and
economic aspects. The chlorophyll content of the leaves indicates the nitrogen status of plants, since there is a relationship between the nitrogen content and the amount of chlorophyll in the leaves. According to plant analysis results there was a strong and significant relationship between the values of the NDVI (Normalized Difference Vegetation Index)and the total nitrogen content measured in the leaves. -
Effect of Irrigation on the Yield and Quantity of Potato Varieties
53-61Views:97In Hungary, the growing area of potato area dropped dramatically in the last few decades. Additionally not only are we lagging behind Western European countries as regards yields, but the competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habits and, often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and some quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2003 and 2004, in two years of significantly different precipitation, on 50 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, from which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
In Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However the effect of irrigation depends on the crop year. In a draughty year, like in 2003, irrigation could increase the yield by 10%, while in a more favourable wet year, the improving effect of irrigation was low. -
The effect of NPK fertilization and the plant density on maize yield and bioethanol production
13-18Views:100For industrial (bioethanol) production of maize, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2008.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
Study of some cooking and eating quality characters on some Egyptian rice genotypes
77-82Views:137Some Egyptian rice genotypes [i.e. Japonica (Sakha 104), Japonica/Indica (Egyptian hybrid1) and Indica (Giza 182)] were investigated to evaluate the cooking and eating quality characters. High significant differences in grain shape were observed among rice genotypes. Hulling, milling and head rice percentage were higher in Sakha 104 than other rice genotypes, while Indica type (Giza 182) recorded the lowest values in milling and physical characters. No significant differences were found in chemical composition of the three genotypes of rice was recorded, but Giza 182 had the highest protein content. All Egyptian rice genotypes were low in gelatinization temperature and soft in gel consistency. Japonica and Indica rice varieties were low in amylase content, while Japonica/Indica rice variety was intermediate. The use of RVA is considered a good index for palatability evaluation for milled rice flour and starch. The Indica and Japonica/Indica types are low in breakdown viscosity, but higher in cooked pasta than Japonica type. Japonica type recorded the best score in panel test, followed by Indica type, while Indica/Japonica rice variety was the least accepted by Egyptian consumer.
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Determining factors of test weight in maize (Zea mays L.)
40-42Views:104Most domestic maize production products are sold on markets abroad. Among the increasingly restrictive quality requirements, the demand for the measurement of test weight has also appeared. This measurement is not unfamiliar in the case of other cereals, such as wheat and barley, but it has not been applied widely in maize. It is likely for this reason that we have such little information and research available on this topic. In this study, we show the current state of this field with references from domestic and international literature.
The density of maize is the weight of a particular volume and the most frequent unit is the test weight (kg/hl). This physical quality factor plays important roles in the storage, transport and mill industries. The value of test weight is influenced by many factors. The most important ones are the moisture content of grains, drying temperature, drought, precipitation, early frost, and the hybrid characters of a given genotype (grain type, FAO number). In general, the grain with higher moisture content has lower test weight and the higher temperature during (above 82°C) desiccation also leads to unfavourable values. Factors such as a drought interval after flowering, early frost in the case of hybrids with higher FAO numbers, injuries by insects, as well as fungal infections also influence the structure and moisture content of the maize grain.
In the future, broader studies (hybrid testing, application of new agrotechnical elements) will be needed for understanding of the factors effecting test weight. -
Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:191Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Changes in Managerial Functions in Agriculture
64-71Views:74Managerial activity is a process constituted by well separable functions, tasks. The functions and the content of managerial activity are influenced by several factors.
This multi-sided affectedness focused our attention on the analysis of management tasks. We aimed to formulate responses for the questions how and to what extent the activities and tasks of organizations define the tasks and functions at the given organization. On the basis of interview surveys, we analyzed the following managerial tasks: functions concerning information, communication, planning, decision-making, organization, control. The interviewed had to rank the factors influencing management tasks on a scale ranging from one to five. On the basis of the activities and tasks of organizations, we defined three types. The scope of this study does not allow us to publish all the results related to the above-mentioned managerial functions, so we can only present some, which are of interest.
Our findings suggest that the realization of certain management functions is influenced by several factors. Without striving for completeness, we could only evaluate 9-11 factors per function. The evaluation of information sources is considerably differentiated in terms of the activities, tasks of organizations. In the assessment of plans, the potentials for realization and repetition were judged differently, whereas non-organization changes and individual planning similarly. The findings of impact factors in decision making are largely different, as well in terms of organizational activities, but all the interviewed ranked the interests of the organization high. Analysis on control indicated that managers attributed higher importance to issues of content than to methodological ones.
Our investigations follow Fayol’s classical groups based on managerial tasks. We studied the following five tasks in the process of management: information tasks, planning, decision-making, instructions and control. We performed our studies with the inclusion of agricultural organization managers, with questionnaire surveys. The interviewed were asked to qualify the impact factors on the five investigated management tasks on a scale ranging from 1-5. We identified the following company groups: small company, medium company and large company.
We performed our analysis on the basis of company sizes as well, seeking to formulate responses on how size influences the qualification of the investigated factors.
Our findings suggest that the interviewed company managers attribute higher than average roles to the tasks of the management process. The qualification, ranking of management tasks are considerably influenced by company sizes. Relationships and venues are important in the implementation of information tasks, whereas the viability of plans is essential in the process of planning. Usefulness and company interests are highlighted in decision-making. In the case of instructions and decision-making, we stressed the significance of content elements as opposed to formal ones. -
The Effect of Year and Irrigation on the Yield Quantity and Quality of the Potato
12-16Views:101In Hungary, the growing area of potato area reduced dramatically in the last few decades, additionally we are lagging behind the Western European countries as regards yields and the competitiveness of production is further decreased by the great alternation in yields from year to year, the unpredictable market conditions, bad consumption habits and many times unfortunately the lack of quality products.
The ecological and climatic conditions of Hungary are not everywhere suitable for potato, in the area of Debrecen the amount of rainfall was lower, and the monthly average temperature was higher than the requirement of potato in its growing season in 2002 and 2003.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment we examined the yield and some quality parameters of 8 and 9 medium-early varieties in large parcels in 2002 and 2003 respectively. Out of the examined varieties 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up on 49.5 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, out of which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the under-water mass, the amount of reducing sugars, the colour index of frying and the element contents of tubers.
Summing up, it can be stated that among the agrotechnical year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. On the basis of our results, it can be stated that in potato production variety should be chosen in accordance with the aim of production and technology should be adapted to that specific variety. -
Effects of fermented chicken manure products on the N mineralization rate of the soil using the incubation method
199-204Views:167In our study, the effect of fermented and specially added poultry manure products (superabsorbent polymer (SAP), bentonite and Aegis as a mycorrhizal inoculum) were investigated in a short soil incubation experiment – at 60% water capacity level - on sandy soil. Soil samples were collected from two layers of the incubation pots after the second and fourth week to check the status of the tested products and the processes in the soil. The pH and the electric conductivity (EC) of the samples were measured using an electrochemical method, while the ammonium and nitrate content of the samples was determined with a photometric method. Soil pH and EC values slightly were decreased during the experiment. Our results pointed out that the increasing dose of SAP caused lower soil pH. The nitrate content of the soil did not change significantly during the experiment. It was found that the increasing SAP content in the products, due to its cross-linked structural property, protected the nitrate ions from leaching. Our results suggest that applied SAP does not bind the nutrient ions so tightly in its structure that it competes with the plant for uptake.
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Changes in the Quality Parameters of Wheat Samples of 16% Moisture Content during Storage
117-121Views:63We have carried out storage experiments with wheat varieties Magvas, Fatima, Mv Emma and Mv Pálma from the 2001 growth year. During the first half of the experiments quality parameters, such as changes in moisture, protein and gluten contents as well as pharinograph readings, falling numbers and sedimentation indices were analysed and tested at an average initial moisture content of 13%.
The findings in the changes in the quality parameters of different wheat varieties with 13% moisture content as a function of storage time duration were published in an earlier paper of mine (12/04/2002).
This paper wishes to give an account of the second part of the storage test when the moisture contents of the different varieties were raised to 16% and the changes in the quality parameters were monitored for seven weeks. The findings show that contrary to what had been experienced in the first half of the trial, there were no changes in the protein contents, pharinograph readings, water uptake or Zeleny counts and falling numbers showed decreases with each of the varieties tested. -
The effect of long-term fertilization on phosphorus content determined in different soil extractants on meadow chernozem soil
7-11Views:68The 0.01 M CaCl2 universal extractant is known all over Europe since the 90’s. During my research, I exam the phosphorus content determined in 0.01 M CaCl2 of the samples originated from the B 17 National Uniformed Long Term Fertilisation Trials in the experimental site Karcag, in the 40th year of the experiment. Relationships between the CaCl2-P and the AL-P content of the soil, the average yields, and the phosphorus balance of the
2006/2007 year were studied. From the results of the study it was concluded as follows:
– Correlation was close (r=0.68-0.7) between the AL-P and CaCl2-P. This is in accordance with the results of previous experiments in Hungary and other countries.
– My studies confirmed that the calcium-chloride method indicates well the deficiency and the surplus of plant available phosphorus. In case of different degrees of negative balance, the amount of CaCl2 extractable phosphorus showed no changes, or decreased, and in case of positive balance it increased exponentially by the long-term effect of P rates.
– The 120 and 180 kg ha-1 rates resulted in significant increases in the amount of CaCl2-P according to the control and treatments that are not fertilized with phosphorus. The 60 kg ha-1 rate didn’t result any increase as it didn’t meet the phosphorus requirement of winter wheat, and presumably the P-balance was negative in the earlier years as well.
– It can be stated that the 0.01 M CaCl2 was able to assess the deficiency and the excess of phosphorus causing negative impacts on environment as well. -
Effect of net shading technology on the yield quality and quantity of chilli pepper under greenhouse cultivation
5-9Views:266This study was undertaken to identify the effect of net shading technology on the total capsaicinoids, vitamin C and relative chlorophyll content expressed as SPAD (Soil Plant Analysis Development) values and total marketable fruit yield of ‘Star Flame’ chilli pepper (Capsicum annuum) for two harvesting times cultivated under modified atmosphere. ‘Star Flame’ pepper was grown under three (3) different net shading colours (white, red and green). Samples without net shading were used as control. Samples were subjected to chromatographic analyses using HPLC for the determination of total capsaicinoids and vitamin C. Interaction in shading technology and harvest times (p=0.010) had significant effect on total capsaicinoids as a result of green and white shading technologies showing higher differences when compared to control samples. Vitamin C content was observed to have increased in white and red shadings (p=0.001) after the first harvest and gradually decreased after the second harvesting time (p=0.002). Relative leaf chlorophyll content was significantly higher in white shading in the first and second harvest. Samples used as control had significantly higher marketable values when compared to white and green shadings with red recording low marketability of ‘Star Flame’ chilli peppers.
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Product orientated sewage sludge composting technologies in the practice of AKSD Ltd.
33-36Views:124In recent years the regulations of the EU unambiguously determine that the biodegradable wastes should be used for biogas production or composting. The direct use of sewage sludge in agriculture is limited by its hazardous microelement content, but it can be used effectively in composting. The limitation parameters can be extinguished with correct compost-mixture and technology variants. The exact determination of the technologies and mixtures result a stable, constant quality product. The compost, as a classified product, is friable structured. The end-product is a
homogeneous mould with good water capacity, does not contain any weed seeds and pathogenic organisms. The compost does not have a acidify effect on the soil. It is an effective fertilizer thanks to its macro and microelement content. -
The effect of increasing compost rates on the yield and nutrient content of ryegrass (Lolium perenne L.)
127-134Views:130Satisfaction of the increasing needs of humanity causes large environmental load. To provide a livable environment for future generations we have to satisfy our needs with the use of sustainable management. This is one of the biggest challenges of nowadays.
The amount of wastes emitted in increasing volume can be decreased by the recycling of them. The disposal of waste materials formed in the public spaces of cities and during the processing of agricultural row materials and by-products in landfills is inconceivable, so they must be recycled.
These materials mostly with organic compounds could be the primary substrates of composts. Completed with suitable additives, and applied appropriate treating technology, composts are capable to supply horticultural plants with nutrients. Composting wastes and byproducts not just decreases the amounts of deposited waste materials, but increases the nutrient (macro- and micronutrients) content of soils, so this is an environmentally friendly and alternative way of nutrient management of plants. -
Motivation for and Possibilities of Increasing Table Beet Root Cultivation
131-135Views:125Due to its manifold nutritional-physiological effects, table beet root would deserve more attention. Its active components and their role in human therapy and prevention should rank it higher in our list of vegetables.
The actual some 100 ha area under beet root could be considerably increased, if its role in nutrition and its varied products were universally known.
Most of the physiologically favourable effects are related to its pigment content. Pigment content can be increased by choosing proper varieties and applying cultural methods which, favourably influence red pigment synthesis (optimal sowing date).
The high Mg-content and advantageous ion ratio must also be mentioned. They can be increased by Mg leaf fertilisation, as can solids content and foliage resistance.
The special knowledge accumulated in literature ought to be made known to experts so as to help them to set up trials and to introduce results into practice. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:125We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.