No 68 (2016)

Published February 18, 2016



Effect of G2548A polymorphism in the leptin gene on the BMI level in human population

The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR ...products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.

The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.

According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.

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Assessment and comparison of selenium-enriched maize with sodium selenite and sodium selenate

Selenium is an element of environmental interest owing to the narrow range between its nutritionally required and toxic concentrations in many organisms. Its mobility and bioavailability differ greatly depending on individual Se species. In this regard, in present study, the uptake and distribution of Se, the changes in Se content, and the effe...cts of different concentration of Se in two forms of sodium selenite and sodium selenate on maize plants were measured in nutrient solution experiments to clarify their response to the two forms of Se. The results revealed that the Se content in shoots and roots of maize plants significantly increased as the Se level increased. Two Se forms behaved differently and the effects of toxic damage in samples which had been treated with selenite were much more than in the selenate treatments.

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Effect of sowing technology on the yield and harvest grain moisture content of maize (Zea mays L.) hybrids with different genotypes

From the aspect of the efficiency of maize production harvest grain moisture content shall be considered beside the amount of harvested grain yield. Hybrids with different genotypes and vegetation period length lose their moisture content different that is affected by row spacing and plant density – among agrotechnical production factors – ...depending on the given crop year. In the present research work three crop years with different weather conditions were studied (2013, 2014, and 2015). The small-plot field experiment was set up at the Látókép Field Research Centre of the University of Debrecen, Centre for Agricultural Sciences with four replications on a chernozem soil type. The effect of three factors was analysed in the experiment on yield amount and its moisture content. Factors were row spacing (45 and 76 cm), plant density (50, 70 and 90 thousand plants ha-1), while hybrids were of very early (Sarolta: FAO 290), early (DKC 4014: FAO 320, P 9175: FAO 330, P 9494: FAO 390) and medium (SY Afinity: FAO 470) ripening.

In the crop year of 2013 the highest yield was produced – regarding the average of the hybrids – by the application of a row spacing of 45 cm (4.5%, 673 kg ha-1), however there was no significant difference between the yield of the populations of different row spacings. Significant difference (14.9%, 1751 kg ha-1; 6.3%, 583 kg ha-1) could be found in case of yield between different row spacing applications in 2014 and 2015. The effect of insufficiently distributed low amount of precipitation and lasting heat days in 2015 could be revealed in yield amounts and harvest grain yield moisture content results that were lower than in the previous years. In 2015 grain yield moisture content varied between 10.3 and 13.9% in case of a row spacing of 45 cm, while by 76 cm between 11.0 and 13.9%.

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The influence of climatic conditions of the harvest year on the wheat quality

Technological potential of wheat bread depends on the variety, manner and type of fertilizer, irrigation conditions and climatic conditions in their absence. This study was done on two varieties of wheat grown in Bihor county in a place with identical fertilization conditions, for two different years: year 2014 can be characterized by a greater... quantity of rainfall during the wheat vegetation period and the year 2015 can be characterized by a drought during the growing wheat on the subject of the study. In the study was examined whether climatic conditions have any influence on the technological potential of wheat, not taking into account the data on quantity produced per hectare in the years studied.

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Economics of hail protection net installation in super intensive apple orchards

The main objective of this study was to determine and organize beneficial and detrimental effects of hail protection nets, and as far as possible to quantify their economic impact. The main factors were determined, through which hail protection nets can affect the costs and incomes of the production, and as their sum the economic result. Consid...ering these factors together with the investment cost and the annual maintenance costs allows the economic evaluation of purchasing of hail protection nets. The analysis was carried out using a deterministic simulation model based on primary data collection from apple producing businesses. Installing hail protection nets as additional technological elements of intensive apple orchards can be concluded by all means as an economic investment – trough their protective effect. However, to achieve really favourable economic indicators, definitely bigger tree height is needed making possible the realisation of around 8.0 t ha-1 extra yield. The main root of this phenomena are obviously the extremely high investment costs of hail protection nets, which result in a large mass of fixed costs during the production period. Therefore the basic economic interest is reaching higher yields and thereby higher profits per unit area.

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Effect of cropping technologies on the yield of dry bean variety ’Diana’

An experiment on three dry bean varieties (Start, Hópehely, Diana), using different sowing-times, fertilizers and plant densities was performed on sandy soil in the University of Debrecen, Centre for Agricultural Sciences, Research Institute of Nyíregyháza in 2015. The aim of the experiment was to study which treatment gives the highest yiel...d, and whether the different treatments result in significant differences in the yields. In this paper the dry bean yield at ‘Diana’ variety is analyzed.

The treatments were done with three fertilizer doses and three plant densities at different sowing-times (April 24; May 8; May 18). As a result of the high temperature and the drought during the growth season, the yields we harvested were in low, which shows the ecological sensitivity of the plant we examined.

We concluded that the poorest yield was harvested at the third sowing-time. There was no significant difference in the yields at the first and second sowing- time. Examining all the three fertilizer treatments we applied at the experiment, we achieved the highest yields in the control plots. It might be due to the weak efficiency of nitrogen fertilizer under the extremely dry conditions. The highest yield was harvested at the control treatments during the second sowing-time. Regarding the effect of the plant densities, the highest crop yield was achieved at the treatment using 400 000 germs ha-1, followed by 300 000 germs ha-1 and 200 000 germs ha-1.

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Effect of agrotechnical factors on the activity of urease enzyme in a long term fertlization experiment

The soil is a natural resource, the fertility preservation is an important part of the sustainable development. We have to monitor the transformation dinamics of the organic nitrogen-containing substances, to get accurate information about the changes of the nitrogen cycle in the soil.

Physical and chemical properties of the soil and th...e microorganism effect on the organic matter in the soil – in addition to the composition of organic matter. Wide variety of extracellular enzymes are present in this decomposition. These enzymes help in the transformation of the macromolecules to transforming low molecular weight compounds so they will be available during the assimilation.

The urease enzyme, catalyzes the hydrolysis of urea to CO2 and NH3. The urease is widely spread in the nature, it is present in the microorganisms, plants and animals.

We found that the soil moisture content, the rotation and the fertilization affect to the amount of urease in spring. Furthermore, we get significant difference between the irrigated and non irrigated samples in the second period of the year. Based on our results we can state that the activity of urease was higher in spring 2014.

The objective of our study was to present how the different agronomic factors affect on the activity of urease in a long term fertilizationexperiment.

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Analyses of a few physiological parameters of hybrid wheat in the case of different nitrogen supply levels

The winter wheat is one of the most determinant crops because its role was always important in human’s life. To increase the average yield there are several possibilities, which are still not clear fields of agricultural plant production. Our main goal was to examine the responses of winter wheat genotypes to different amounts of nitrogen sup...plies. The sowing area of hybrid wheats are increasing, they may have different nutrient nitrogen utilization compared to varieties, and the question arose if it is possible to achieve same yield at lower nitrogen fertilizer application or not.

The present study analyzes the results of winter wheat (Triticum aestivum L.) from tillering growing stage. Under controlled conditions three different wheat hybrids were grown (Hywin, Hystar, Hybiza) with two different amounts of nitrogen supplies (optimal and the fourth part). The dry matter accumulation, relative chlorophyll content and nitrogen content were measured in order to draw conclusions from the different supplies of nitrogen for winter wheat genotypes and their physiological plasticity.

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Chestnut tree: an appropriate choich for rural developement in Europe (review)

In chestnut plantations or in associations many pathogens and pests can be found. They are causing more than a hundred year problem in chestnut protection, for example the blight fungus Cryphonectria parasitica. Lot of breeders hybridized the Castanea sativa and Castanea dentata with the tolerant asian species. Nowadays the presence of kuriphilus seems to be the main risk. However, there are more and more chestnut plantations in Europe because of the extensive growth of needs. The food industry produces many products from chestnut, but the wood industry also use it more and more. In most of European countries the chestnut stands represent landscape aesthetics and touristic value, but probably the number of farmers inquiring the plantation will be increase in Hungary.

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The examination of the marigold’s (Calendula officinalis L.) nutrient requirement in small-plot trial

During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization treatments in small-plot trial. We measured the harvested marigold's drug's raw and dry weight on a weekly basis from July 6th until August 17th. We were using SPME (Solid phase microextraction) ...and GC-MS (gas chromatograph-mass spectrometer) to examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.

It was concluded, based on the results, the N30P40K60 fertilization setting is ideal in terms of the quantity of the marigold drug. Both the raw and the dry weights' measurements of the case, this fertilization setting have the most important effect on the herb's yield. The analysis of variance didn't show significant differences between the plots with different fertilization settings. We discovered relationship between the drying loss and the increasing quantities of nutrients. We think it may be possible the Alpha-thujon's and Alpha-cadinol's production and the drying loss's data are connected, which appear to confirm the N15P20K30 treatment's data.

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The effect of production area on the development of yield producing factors of maize (Zea mays L.) hybrids of different genotypes

Maize yield amount development is determined by the given crop year and the genotype of the applied hybrid, but beside these also by the applied agrotechnical factors, in particular by sowing technology. The development of yield amount and yield producing factors of five maize hybrids of different genotypes has been studied in a small-plot fiel...d experiment by the application of different row spacings and plant density variants. The production of the individual plants shows decreasing tendency parallel to the increasing plant density, however, this decrement is compensated by the higher number of plants per unit production area. Individual plant production is determined by the development of yield producing factors, such as the length and the diameter of cobs, just as by the thousand seed weight – that were studied in the present research work as well.

In the present research work the decreasing row spacing resulted in a yield increment of 0.67 t ha-1 (4.53%) in 2013, while in contrast in 2014 yield was decreased by 1.75 t ha-1 (14.87%). The high amount of precipitation in March was determinant in 2013: it filled up the soil water stock and balanced the negative effect of the inadequate amount and distribution of precipitation during the vegetation period for the yield. Lower extent of yield increment (0.6 t ha-1) was registered in 2014 in case of the row spacing of 76 cm than in the previous year. In case of a row spacing of 45 cm the difference between the two crop years was 3.1 t ha-1. The highest impact on the yield production factors was found in all treatment combinations in case of the applied hybrid among the three studied treatment factors. In the crop year of 2014 the effect of plant density on cob diameter and thousand seed weight could be revealed as well. In case of the cob diameter significant difference was found between the plant densities of 70 000 and 90 000 plants ha-1, just as between the populations with densities of 50 000 and 90 000 plants ha-1. In case of the thousand seed weight significant differences could be found by the application of plant densities of 70 000 and 90 000 plants ha-1. The highest values of the studied yield producing factors were measured in case of the plant densities of 50 000 and 70 000 plants ha-1; increasing the plant density to 90 000 plants ha-1 resulted in rather decreasing values.

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Testing of paraffin oil efficiency against grape powdery mildew in Eger wine region

The aim of the present study was to examine the efficiency of paraffin oil against powdery mildew in Eger wine region. The experiment has been carried out in 2013 and 2014 with Chardonnay and Kékfrankos grape varieties, which have different resistance against powdery mildew. The effectiveness of the oil was examined on leaves and clusters (fre...quency and intensity). This oil was effective against Erysiphe necator infection on field trials in Chile and Brazil. The spread of downy mildew (Plasmopara viticola) was also inhibited by this material in some experiments conducted in Spain and France.

The differences between oil treatments represented the sensitivity of the grape varieties in accordance with the applied dosages. The oil was effective against powdery mildew with different extent as a result of the so called ”vintage effect”. In 2013, the treatment of the highest dosage (D3) didn’t differed significantly in frequency and intensity of infection from the regular treatment (clusters of Kékfrankos, leaves of Chardonnay). In 2014, the oil was not so effective against powdery mildew compared to 2013. No remarkable differences were detected between the treatments due to the strong pressure of powdery mildew. Furthermore, no any effect of the lowest dosage (D1) was detected in the case of the sensitive clusters of Chardonnay and leaves of Kékfrankos in both experimental years.

In summary, the oil treatment has an effect against powdery mildew, however this efficiency largely depends on the vintage characteristics and the pressure of powdery mildew. Further investigations are neccessary, for example field trials with combinations of other sprays. The oil can be useable as fungicide with proper care in eco-friendly integrated and bio (ecological) viticulture.

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Development opportunities for storing and displaying of spatial data in long-term experiments

Long-term experiments are required to evaluate the impact of irrigation, nutrient utilization, and year factor as well as to assess the potential consequences of climate change. However, in the long-term experiment, it may be necessary to display spatial data for each parcel, either for investigation of soil heterogeneity or presentation. This ...article aims to provide help for researchers working in long-term experiments for storing and displaying spatial data.

After the outlines of each experimental site were measured with GPS, a spatial database has been created in Quantum GIS. Then, a filter script in R statistical environment using RStudio graphical interface was written. The script helps avoid the QGIS data input interface so that large data can be attached to each parcel directly and as a result there is no need for a separate data entry, only the basic statistical database.

The created GIS database can be used in many ways; it can be exported to KML file format that can be displayed using Google Earth. It is possible to view exported KML files in Google Drive with importing them to Google My Maps application, and with that a browser can display the map. With the Google Drive the maps can be shared within the research group, additionally the outlines can be edited and it is possible to upload the measurement data to the attributes table to existing empty table columns. The map created in Quantum GIS can be used for presentation purposes.

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Toxic elemental content of Hungarian blaufränkisch wines

Toxic elemental contents are one of the food safety risks in wines. Therefore International Organization of Vine and Wine (O.I.V.) defined the limit of some elements in it. Thirty Hungarian blaufränkisch wines were analysed by ICP-MS in order to determine the concentration of toxic elements. All wines are passed by the O.I.V limits to toxic el...ement content (Cu, Zn, Cd, Pb) according to analysis. Copper is the only outlier in some samples, but they also did not overstep the limit. In the case of zinc the maximum is approximately fourth, in case of cadmium and lead the highest concentration is less than tenth and sixth of the O.I.V. limit, respectively.

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Economicalness of self-propelled work platforms in superintensive apple plantations

The main goal of this study was to determine whether the use of work platforms in super intensive apple orchards in order to reduce production costs and enhance work area performance under Hungarian circumstances an economic investment is. The analysis was carried out using a deterministic simulation model based on primary data collection from producing businesses. The results show that work platforms have remarkable economic advantage in case of operations with high machine work expenditures or where they significantly improve the area performance of manual labour. The former was due to the much lower operating costs of work platforms compared to tractors, while the latter comes clear from reduction of manual labour input per hectare. The greatest economic advantage of work platforms occurs in case of harvesting and pruning, but in case of other operations they have no significant cost advantage. They main economic disadvantage is, however, that apple breaks more when harvesting with work platforms, i.e. picking quality deteriorates significantly. It can be concluded, that purchase of work platforms cannot be considered economic investment under Hungarian circumstances. It might be economic, utilised on maximum area (ca. 30 hectares) by certain companies in special cases, but in most cases has no favourable payback conditions.

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Physiological plasticity of main tree species of lowland hornbeamoak forest as a results of forest gap regeneration

Specific leaf area (SLA) of English oak (Quercus robur L.) and hornbeam (Carpinus betulus L.) as members of Querco robori-Carpinetum were investigated in two different habitat in terms of gap forest management: in the gap and in the inert forest. The artificial opening process of the forest resulted in more light for growing saplings and need f...or acclimatization. Photosynthesis is one of the most important ways for plant life and plant production basically influenced by altered light condition resulted in opening process. Efficient photosynthesis is important for plant life, plant production, but species-dependent plasticity of photosynthesis makes one species more tolerant, than others. The specific leaf area is acceptable parameters for characterising plant production, dry matter content and leaf structure. The dry matter content based on known leaf area is higher in oak both sun and shade leaves, than hornbeam. The different place of leaves in the canopy of trees did not influence the values of SLA.

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The effect of sowing date and plant density in three maize hybrids germination and growth dynamics

The maize research was set up on chernozem soil at Látókép research area of the Centre for Agricultural Sciences University of Debrecen. We examined the following hybrids SY ARIOSO (FAO 300), P9486 (FAO360), DKC 4943 (FAO 410). The experience was set u pin three different plant density. These were 60, 76 and 90 thousand plant ha-1.... The experience was set up in three different sawing date, early, average and late. The germination and growing dynamic measurements was measured in three hybrid, three sawing date, three plant density in four replication. well observed at the first sawing date (April 5) the soil was too cold therefore the germination was begins very slowly to be slowly increased. The second sowing time was the average (April 21) there the germination launch as soon as possible more rapid growth in the amount of heat. We experienced the most intense germination was in the case of the emergence late sowing date (May 5). Looking at the growth dynamics for the first two sawing date was side by side and almost equal to the maximum value. This is explained by the adaptive capacity of the maize to compensate for the sawing difference. For the third time, despite the delayed sawing the maize began to grow more dynamically than in previous sawing times due to the results of the initial good conditions it growth faster than halted in the second half of the season because of the high temperatures and lack of precipitation.

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Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil

We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on... 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.

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