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  • Changing of carbohydrates by inoculation of Pseudomonas savastanoi pv. phaseolicola oil bean lines with different resistance
    82-85.
    Views:
    135

    The Pseudomonas savastanoi pv. phaseolicola (PS) is one of the most significant stressors of bean (Phaseolus vulgaris L.). Chemical and agrotechnical treatments have minor importance, so breeding has great part in the protection against this pathogen. Most of the cultivars are susceptible to PS. The genetic background of resistance in the plant is a complex system. Leaf resistance is a monogenic system, but there are some modifier genes. The pathogen species can be divided into different races.

    To understand the functioning of this resistance gene, experiments were carried out using bean varieties with different genotypes and near isogenic lines of bean. Eight lines were tested. Our main objective was to test bean lines with PS with high virulence.

    The experiment was made in greenhouse and on field. The virulent bacterium strain has been isolated in Hungary.

    The changes of carbohydrates were tested after infection. In homeostasis the level of carbohydrates (especially glucose and fructose) were higher in susceptible lines. In case of artificial and natural infection the decrease of glucose were more significant in susceptible lines than in resistant lines. In the leaf samples from systemic chlorosis the level of this carbohydrate increased.

    These changes are connected with the level of resistance, but more experiments are needed to verify this assumption.

  • Yield and composition of supercritical fluid extracts of different Lamiaceae herbs: Satureja hortensis L., Ocimum basilicum L. and Melissa officinalis L.
    75-79.
    Views:
    165

    In our work the effectiveness of supercritical carbon dioxide extraction was studied on volatile and nonvolatile components of Lamiaceae herbs. The aim of our investigations was to determine the optimal extraction parameters yielding high amount of volatiles and nonvolatiles in a desirable composition. As plant materials, dried and powdered cude drugs of Satureja hortensis, Ocimum basilicum and Melissa officinalis were chosen. Pressure (8-50 MPa), temperature (35-60 °C), time (5-60 min) and the ratio of modifier (5-50% methanol) of extraction were regulated. Results obtained by supercritical fluid extraction (SFE) were compared to that of the conventional extraction procedure, hydrodistillation (HD). In the case of Satureja hortensis, Ocimum basilicum and Melissa officinalis extract yield of SFE was comparable to the essential oil amount obtained by hydrodistillation from the same drug. Essential oil rich extracts were analysed by GC-FID, while extracts rich in nonvolatile compounds were analysed by HPLC. We have found that yield and quality of SFE extracts highly depend on the conditions of extraction.

  • The effect of climatic anomalies on the nutrient supply of fruit plantations (Minireview)
    111-116.
    Views:
    219

    Climatic conditions play an important role in agricultural production. It has a profound influence on the growth, development and yields of a crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water stresses. Nowadays, we have to know the dark side of the weather events because it is causing more and more problems and significant hazards to many horticultural regions in Hungary. The aim of this study is to explore the problems of nutrient uptake following climatic anomalies and response. These problems are: (i) water supply problems (water-stress); (ii) drought and frost as temperature­ strees. Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:

    • Nutrient demand of trees can be supplied only under even worse conditions.
    • The most effective weapon against damage of climatic anomalies is preventative action.
    • When developing a fruit orchard, three factors should be taken into consideration: "Location, Location, Location".
    • Moreover, proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of climatic anomalies.
    • Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
    • Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as modifier factors.

    Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar fertilization is recommended, which adjusted to phonological phases of trees.

    Another solving is groundcover of soil means a potential opportunity to temper or even avoid climatic anomalies.