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Optimization of sample preparation for determination of antioxidant parameters from one grape berry
93-97.Views:443Berry quality is an important issue in wineprocessing, however evaluating characteristics in a single berry level is very complex. The Phenolic compound analysis is more challenging because of the limited amount of material and a mixture of skin, pulp and seed. No clear protocol exists for evaluating these compounds from single berries. The aim of our investigation was to develop such a protocol. Single whole grape berries samples were homogenized in 20 replicates by 5 different methods. The most effective method was when berry was placed in a mortar and was crushed with a pestle under liquid nitrogen until seeds were clearly visible. Seeds were then taken from the mortar and crushed between two stainless steel plates with a hammer, and returned to the mortar without residues. Homogenization continued until the sample had a powdery appearance. The homogenized samples were taken for further analysis such as total polyphenols, total anthocyanin contents and total antioxidant capacity. Our results demonstrate that single berries could be processed and that the homogenates were suitable for taking chemically uniform subsamples.
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Effect of the terroir and the harvest time on the Kékfrankos grape berries visual and physical parameters in Eger (Hungary)
81-85.Views:275In this research, the effects of the “terroir” and the harvest time on the most important physical properties of the berries of Kékfrankos (Blaufrankish) grape variety were examined in 2010 and 2011. The results of the experimentation shown, that the maturation processes can be well described by different physical parameters of the grape berry. The terroir and harvest time effects were investigated in two different vintages and four different territories. It can be concluded, that these physical parameters change significantly during the ripening period, but trends differ between vintages and regions.
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Effect of the terroir and the harvest time on the Kékfrankos grape berries sensorial properties in Eger (Hungary)
87-92.Views:337In this research the effects of the production site “terroir” and the harvest time on the most important organoleptic properties of the berries of Kékfrankos (Blaufrankish) grape variety were observed. The follow-up of the maturation process in a given parcel can effectively supplemented by the results of sensorial evaluations of the grape berries. In most cases, the tasters found significant differences in sensorial characteristics of the berries due to the harvest time, although the trends of each parcels were different. Significant differences were frequently found in the result of the observed terroirs.
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Study on the Penicillin-content of botrytized wines and noble rotted berries in Tokaj-region
91-94.Views:412Favourable parameters are offered to the formation of noble rot caused by Botrytis cinerea by the climatic conditions, soil circumstances and grape-varietes of Tokaj-region. As the result of noble rot, aszú-berries represent the most important raw material of the world-wide known Tokaji Aszú Wine. The objective of this study was to measure the penicillin-content of aszú-berries and Tokaj Wine Specialities. As the result of infection of Botrytis free way can be opened in front of other mould strains, especially Penicillium and Aspergillus. There is a possibility to the presence of Penicillium chrysogenum on the surface of aszú-berries during noble rotting process. P. chrysogenum was found to produce the so called Naturally Derived Penicillins such as Penicillin-V (phenoximethyl-penicillin).
According to our findings the Penicillin-V content was detected between 0.4 and 26 mg/1 wine and 0-74 mg/kg aszú-berry. These values do net have therapeutic effect, but physiological benefit and it can be a basis to the qualification of the raw material of Tokaj Wine Specialities naturally together with the other component of noble rotted berries and botrytized wines.
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Study on the yeast and mould biota of the botrytized grapes in Tokaj region in two years
61-65.Views:290The famous wine Tokaji Aszú is produced from "noble rotted" grapes infected and modified by Botrytis cinerea under special condition. The objective of this study was quantitative and qualitative characterisation of saprophytic fungi present on the surface of aszúberries, with special regard to yeasts. There were considerable differences in these populations depending on the origin of noble rotted berries, notably between berries taken from the vine or the ones taken from the winery. Beside Botrytis, other mould species like Penicillium, Aspergillus were commonly found, in widely varying population. Yeast counts were detected between 104 and 107 cfu/g berry. In the samples taken from the vineyard Candida pulcherrima predominated followed by some aerobic basidiomycetous species, but Hanseniaspora species were also present in relatively high population. After transport and storage of the grape in the wineries, population of aerobic yeasts and C. pulcherrima quickly declined and Candida stellata followed by other sugar tolerant species became dominant. This autoselection process directs attention to the importance of storage conditions.