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Review of the self-incompatibility in apple (Malus x domestica Borkh., syn.: Malus pumila Mill.)
31-36.Views:883Apple (Malus x domestica Borkh.) is one of the most important fruit crops showing ribonuclease-mediated self-incompatibility, and no self-compatible apple cultivars are known. Twenty-nine S-alleles were identified in apple and many more incompatibility groups are present compared to sweet cherry. Results from a Belgian, English and a Japanese research group are combined and the S-genotypes of the most important world cultivars are collected. Two different allele labelling system are reconciled and detection methods used in case of the specific alleles are shown. Effects of the resistance breeding programmes are discussed; and scientific efforts involving transgenic technology to create self-compatible genotypes are shown. This review covers the most interesting issues regarding self-incompatibility in apple.
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Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation
53-56.Views:286The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.
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Comparative anatomical study of leaf tissues of scab resistant and susceptible apple cultivars
43-45.Views:333According to previous studies some anatomical features seem to be connected with resistance or susceptibility to scab caused by Venturia ineaqulis (Cke./Wint.) in case of a given cultivar. Study of leaf anatomy of three scab resistant (‘Prima’, ‘Florina’, MR–12) and two susceptible (‘Watson Jonathan’, ‘Golden Delicious Reinders’) apple cultivars have been made. Preserved preparations made of leaves has been studied by light microscope. Studied parameters were: thickness of leaf blade, thickness of palisade and spongy parenchyma, thickness of epidermal cells, thickness of the cuticle. By measuring leaf thickness and epidermal cell thickness visible differences appeared in certain cultivars, while most conspicuous difference has been shown in thickness of the cuticle.
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Left, right, up and downstage: leaves and lateral roots histological trait prospection for drought tolerance in commercial Coffea arabica cultivars
44-65.Views:668The climate change and water deficit challenges plant producers all over the world, and have consequences to coffee production and quality. In this research we have approached anatomical traits from vegetative organs of 13 Coffea arabica genotypes, selected based on their contrasting behavior to water deficit. Leaf blade, petiole and primary root cross sections were evaluated, and the epidermal, fundamental, and vascular tissues descriptive anatomy, histometric and histochemistry examined. Despite all plants were in the same environment (CEPC/EPAMIG, Patrocínio, MG, Brazil), there were differences among the genotypes and groups of more tolerant and more susceptible accesses. Petiole cross section, vascular tissue and phloem and cambium; and percentage of stele, pericycle and phloem and cambium in primary roots exhibited differences among the contrasting genotypes, highlighting an inborn association of vascular tissue and other features with water deficit resistance. This association was observed in the mild to medium correlations among vascular tissue, epidermis, phloem and cambium in roots and petioles. Possible relation of qualitative traits such as the lignification of root epidermis, lipidic substances in outer cortical cell layers, and area/number of cell layers in the cortex are approached as possible traits in the seek for water deficit tolerance in C. arabica.
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Impact of foliar fungi on dogroses
23-30.Views:334Wild roses of the section Caninae, commonly known as dogroses, have been described as more disease tolerant than ornamental roses and could therefore become valuable for breeding improved rose cultivars. Two fields with dogroses, one with plants obtained by open pollination in wild populations, and one with plants obtained from intra- and interspecific crosses, were evaluated for blackspot, powdery mildew, rust and leafspots in the autumn of 2005. Symptoms of the different fungi on different dogrose species were carefully evaluated in a microscope and documented by photography. Interestingly, almost no symptoms of powdery mildew were found in either field, although the fungus infected wild roses of a different section in a field closeby. Surprisingly few symptoms were found also of blackspot, and they differed considerably from those found on ornamental cultivars, indicating a lower susceptibility in dogroses. The most important fungal disease in 2005 was rust, followed by leafspot symptoms. The latter were apparently caused by Sphaceloma rosarum and Septoria rosae which can be properly discriminated only in a microscope. The investigated dogrose species and their progeny groups varied significantly in disease susceptibility and in the appearance of encountered symptoms but there was no evidence of major resistance genes, except possibly in Rosa rubiginosa which did not show any symptoms of Septoria. In 2006, a subset of the plant material in Field 1 was evaluated to check for consistency between the years. Leafspots had overtaken rust as the most important disease but results were otherwise very similar to those of 2005.
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Reactions of different plant organs of pear cultivars to Erwinia amylovora infection
17-21.Views:314Research project has been initiated in 1999 with the aim of evaluating the degree of susceptibility/resistance of pear cultivars grown in Hungary to fire blight disease caused by Erwinia amylovora. The recently selected promising cultivars were also examined. Inoculation experiments were conducted in controlled greenhouse conditions because of quarantine regulations in Hungary. Following the disease process, development of symptoms of plant organs (shoots, flower parts, fruits) was observed. Suspension of two E. amylovora strains (Ea 21, Ea 23) isolated from pear was used in a mixture (5x108 cells x m1-1) for the inoculation. Twenty-six pear cultivars were examined and grouped into four categories: low susceptibility, moderately susceptible, susceptible and very susceptible. Most of the cultivars were susceptible or very susceptible while some promising 'Eldorado', 'Harrow Delight' and `Hosui' showed low susceptibility.
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Biological performance of grape varieties in Eastern Hungary
31-35.Views:380Eurasian and interspecific grape varieties and candidates were inspected for biological productivity in the variety collection of the University of Debrecen at the Horticultural Experimental Station in Pallag. In this paper, data are reported on yields (kg/stock), cane production (kg/ stock) and use-up index of wood yield of each cultivar in the experimental years 2011-2012. From the range of Eurasian (Vitis vinifera L.) white wine-grape qualified cultivars and candidates ’Ezerfürtû’, ’Generosa’, ’Jubileum 75’, ’Müller Thurgau’, ’Zöld veltelíni’,’B-11’, ’CSFT-92’, ’Pintes’, from the range of Eurasian (Vitis vinifera L.) red wine-grape qualified cultivars and candidates ’Kármin’, ’Pinot noir’, ’Alicante Bouschet’ and ’Rubintos’ were highlighted. Promising data of interspecific ’Aletta’, ’Csillám’, ’Kunleány’, ’Orpheus’, ’Refrén’, ’Taurus’, ’Viktória gyöngye’, ’Alföld 100’, ’Reform’, ’RF38/32’, ’Toldi’ white, and ’Dunagyöngye’ ’Pannon frankos’ red wine-grape cultivars and candidates were emphasized. Published data refer only to the biological performance of the cultivars. The thorough evaluation of varieties needs respect to data on resistance to fungal disease and climatic extremities, and other characteristics concerning production technology and oenological parameters.
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Efficacy of selected botanical extracts against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) on tomato
31-36.Views:534Tuta absoluta Meyrick, also known as the tomato leaf miner, is a destructive pest that feeds on tomato plants, causing significant economic losses to tomato farmers globally. Traditional methods of controlling Tuta absoluta involve the use of synthetic pesticides, but these methods have drawbacks such as environmental pollution, development of resistance by the pest, and negative effects on non-target organisms. As a result, there is a growing interest in the use of botanical extracts as alternative and safer pest control options. Three bioassay experiments were conducted to test the insecticidal, antifeedant and repellency effects of botanical extracts on Tuta absoluta. The experiments were conducted as completely randomized design (CRD) with three (3) replicates. There were eleven treatments; pyrethrum, neem, warbugia, each at different concentration levels of (8%, 10%, 12%) and controls positive (Indoxacarb 150g/L) and negative (distilled water). In all the bioassay conducted, the effects of all treatments increased over time, except for distilled water control. Synthetic chemical Indoxacarb exhibited significantly stronger insecticidal properties compared to neem, warburgia and pyrethrum extracts. Application of neem at 8%, 10% and 12% and pyrethrum at 12% had higher antifeeding effect on Tuta absoluta compared to the other treatments while warburgia at 8% concentration and indoxacarb had the lowest repellency effect compared to the other plant extracts.
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Exploring and preserving old apple cultivars of the Carpathian basin
9-13.Views:318In a few scattered orchards of the Carpathian basin one can still find our most common historical apple cultivars and certain traces of traditional cultivation. We consider it an important part of the Department's breeding work to explore old apple cultivars which can be used as parent partners and sources of resistance, to identify them pomologically as well as to examine the production and marketing value of old cultivars recommended for cultivation in ecological production and in gardens. This paper documents the results of expedition and collecting work carried out in three regions of the Carpathian basin (foot of the Carpathian Mountains, Transylvania, Aggtelek karst area), and also the saving of old Hungarian apple cultivars preserved until present in the English National Fruit Collection. A total of more than 100 old apple cultivars and variations, as well as 13 valuable genotypes were collected during our explorations in the above mentioned four areas. A further aim of our work is to contribute to the protection of still savable archaic fruit production relict= areas and to the preservation of cultivars in the certain regions, and also to promote the recognition of the aesthetic landscape value of old apple trees and endemic orchards of old cultivars. Our good co-operating partners in this work are Aggtelek National Park, Farmer Club of Visk and the consultation centre of Hungarian horticulturist training beyond the border in Nyárádszereda.
