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Comparison of Xanthomonas arboricola pv. juglandis isolates from walnut trees grown in Romania and Hungary
65-69.Views:286Among diseases that affect walnut, bacterial blight is considered the most important one in all walnut growing areas both from Hungary and Romania. For the determination of susceptibility/resistance of walnut cultivars in our posterior work planned, 61 bacterial isolates were collected from walnuts showing symptoms of blight, with the purpose of isolating Xanthomonas arboricola pv. juglandis (Xaj). The characteristic Xaj colonies, frequently present as saprophytes in the infected plant tissues, were separated from other bacteria according to their morphology, yellow colour, hydrolysis of starch, and oxidation of glucose. All isolates were tested for pathogenicity by hypersensitive reaction on tobacco leaves (Nicotiana tabacum L.), bean pods (Phaseolus vulgaris L) and unripe nuts (Juglans regia L.). Determination of taxonomy of the selected isolates denotes a possible subdivision (races, biotypes) of Xaj occurring in different geographical areas, API 20NE and API 50CH kits were used. Hungarian and Romanian strains showed a high degree of similarity of carbohydrate utilization but slightly differed from the type strain. All were Cu-sensitive.
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Precision geoinformatical system of the pear gene-collection orchard
43-50.Views:239The principle task of the sustainable development is the preservation of the genetic variety, which is similar challenge in the horticulture regarding the sublimation of fruit species. The breeders of the traditional fruit strains give stock to the sustenance diversity of the agro-environment on the species and landscape level. In 2009, hyperspectral images have been taken by AISA Dual sensors from the pear gene pool in Újfehértó, Hungary. The hyperspectral data cube (in the wavelength range of 400-2500 nm, with 1.5 m ground resolution) ensured possibility to make the spectral library of pear species. In the course of the simultaneously field work the spatial position and individual extent of all pear trees was defined to set up a detailed GIS data base. The water stress sensitivity of single species and the descriptive spectral curves were determined with common evaluation of the spectral and spatial data. Based on the unique methodology processing and the hyperspectral data base suitable strains can be chosen for agro-environment and let take adaptive stocks regarding climate change into the genetic grafting work. Furthermore we could determine and map the sparsely species in the region with the help of the hyperspectral data.
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Sunburn incidence of apples is affected by rootstocks and fruit position within the canopy but not by fruit position on the cluster
45-51.Views:278Authors investigated sunburn incidence of apples on the combinations of three different growth inducing rootstocks (M.9,MM.106 and seedling) and five varieties (‘Smoothee’, ‘Golden Reinders’, ‘Granny Smith’, ‘Gloster’ and ‘Jonagold Jonica’). Symptoms were classified as sunburn browning, sunburn necrosis and photooxidative sunburn. The frequency of symptoms was recorded at various parts of the canopy (N, E,W, S, and lower canopy, upper canopy) and on the cluster (terminal, lateral). Cultivar susceptibility varied between 0.30 and 5.65% on M.9 rootstock, ‘Granny Smith’ seemed to be the most susceptible cultivar whereas relatively low percentage of damaged fruit was observed for ‘Gloster’. On MM.106 and seedling rootstocks, damage level was significantly lower than on M.9. Remarkable differences were not observed in the share of the three sunburn types between cultivars. The most common symptom observed was sunburn browning. Far less fruit was affected by sunburn necrosis and photooxidative sunburn. Photooxidative sunburn symptoms were not found on ‘Granny Smith’ and ‘Gloster’ fruits on MM.106 rootstock. Latter cultivar did not show sunburn necrosis symptoms either. With increasing growing vigor of the rootstocks the share of sunburn browning increased. Fruits with sunburn symptoms were found in a great majority on theW quadrant of the trees. This was true for all cultivars. Remarkable differences in the location within the canopy of affected fruits between the three types of sunburn were not observed. Specific distribution of sunburned fruit was observed along the vertical axis of the canopy, too. Most of the damaged fruit were found in the upper canopy. This is particularly true for trees on vigorous stocks such as MM.106 and seedling. On M.9 rootstock, depending on cultivars 5.9 to 38.9% of sunburned fruit was located in the lower canopy. Most common symptom in the lower canopy was the sunburn browning, however symptoms of sunburn necrosis were not found at lower canopy level. Low rate of photooxidative sunburn was observed such lower canopy conditions. Sunburn incidence was very similar on king or side fruit. Significant differences were not found in the share of each sunburn types between fruit positions on the cluster. This was not influenced by rootstocks either.
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Study of Erwinia amylovora colonization and migration on blossoms of susceptible and tolerant apple cultivars
15-19.Views:154The stigmata of detached flowers of susceptible and tolerant apple cultivars were inoculated with about 104 gfp labeled Erwinia amylovora . There were no apparent differences in the colonization, multiplication and survival of the bacteria on the stigmatic surface of the culivars. Bacteria were washed down to the hypanthium surface 24 hours after inoculation. The visual symptoms of the infection were the discoloration and shrinkage of the floral parts. The gradual browning associated with the infection appeared first on the surface of the hypanthium followed by the discoloration of the style. The color of the filaments turned into brown only 120 hours after the inoculation. Bacterial cells were not detected in the tissues of the styles and filaments. The traits of the hypanthium surface are of prominent importance in the progression of the infection. The wrinkled surface, the convex shape of the outer epidermal cell walls with thin cuticle and the sunken stomata helped to preserve a water film for a longer period providing medium for the motility of the bacteria in the susceptible cultivar. Bacteria were restricted to small water droplets on the flat and waxy surface of the hypanthium of the tolerant cultivar and only a few were able to enter the tissues.
Large bacterium aggregations were detected in the intercellular spaces of the parenchyma of the susceptible cultivar 48 hours after the inoculation. In the next period the Erwinia amylovora cells gradually invaded the intercellulars of the hypanthium wall, the wall of the ovary and the pedicel. Low level of bacterium aggregation was found in the intercellulars of the tolerant cultivars. It is suggested that the progression of the infection was inhibited also by physiological factors.
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Studies for using frameless plastic in the forcing of some ornamental crops
148-150.Views:148On the basis of a six-year experiment a method was elaborated for forcing and off-seasons growing of tulips, narcissus and gladioli under frameless plastic cover. The advantages of this cheap energy-saving method are manifested in an improved quality of flowers and essentially in the earlier flowering. With this method mostly first-class flowers can be obtained immediately after the season of forcing in heated constructions with plastic cover, and anticipated outdoor flowering.
The possibilities of applying the method are studied on further ornamental plant species (other bulbous plants, Paeonia lactiflora, annual ornamentals).
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Comparison of honeybee behaviour in blooming fruit plantations
147-151.Views:303Field observations were made on the fl ower visiting behaviour of honeybee foragers in commercial fruit plantations of apricot, Japanese plums, sour cherry, apple and pear. The number of inspected cultivars was 18. The intensity of fl ower visiting by honey bees was markedly different when data of different fruit species are compared. Most intense bee activity was registered on the Japanese plums, somewhat less on apricots, the intensity diminished signifi cantly with apples and pears. Our data presented on the honeybee visitation of Japanese plums can be regarded as new fi nding because no information has been available so far on the relative attractiveness of this fruit species compared to European fruit tree species. Japanese plums were somewhat more attractive to honeybees than apricot and much more attractive than sour cherry, apple and pear. The behaviour of honeybees as visiting the blooming trees displayed specifi c differences according to the fruit species (apricot, sour cherry, pear), which coincide largely with earlier results. It is notable that the fl ower visiting behaviour of honeybees on Japanese plums has been found to be fairly similar to the same on European plums.
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Comparison of apples from organic and integrated farming
15-18.Views:316Prima’, ‘Gala’, ‘Remo’, ‘Topáz’, ‘Idared’, ‘Releika’, ‘Resi’, ‘Rubinola’, ‘Rajka’, ‘Rewena’ and ‘Florina’ apple cultivars, both from organic and integrated farming, from Pallag and Újfehértó, were compared. Average size, weight, soluble solids, titratable acidity, total polyphenols, free radical scavenging capacity expressed as Trolox equivalent (TEAC), copper and zinc were determined at harvest and after cool storage. Organic apples were more acidic, while integrated fruits had mostly higher copper and zinc content. Total polyphenols and TEAC values did not show a significant difference as a function of farming technology. A principal component analysis shows the separation of provenances as well as stored and fresh apples. Results are considered as preliminary.
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Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants
7-10.Views:233Up until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem.
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Work organization and economic analysis of the postharvest of an unique Hungarian product
11-14.Views:159We have viewed a business in the south of the Great Plain Region from an economic point of view in 2004-2005. The main activity there is pear growing and storage. Four varieties of different time of ripening and storing are grown there. We have measured all the relevant activities, worked out a local normative and prepared a detailed technology. The economic evaluation was based on this data. Activities, like disinfection, pre-storage disinfection and selection, in-storing and out-storing, classification after storage, packaging, as well as loading trucks, were monitored by variety. Storage loss was determined and widely varied according to varieties, length of storage and time of out-storing. Different varieties resulted in different quality classes after storage. Price depended on the quality classes. Economic evaluation was carried out when all the relevant costs and revenues were known. Fixed and variable costs of storage were determined, break-even point was calculate and the market position of the product was evaluated.
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Volatile constituents of Nepeta cataria L., N. glechoma Benth. and N. parviflora M. Bieb. from Hungary
47-50.Views:128In the temperate zone live about 150 species of the Nepeta genus. Our investigations covered the examinations of the volatile oil containing species of the genus endemic in Hungary, Nepeta cataria and Nepeta parviflora. Latter is a relict of the ancient steppe-flora and endemic in Hungary as well. Phytochemical examination of the volatile oil containing plant material has also been carried out. Catnip growing in the Botanical Garden of PTE Department of Botany contained 0,67% volatile oil in May and 0,14% in November. Chemical character of the volatile oils were measured by gas chromatography/mass spectrometry and citronellol, citral-A, citral-B and geraniol components were identified. The composition of the oil of November samples shifted towards citronellol (65%). In both samples insecticide and repellent activity bearing compounds (+)-cis-p-menthane-3,8-diol, and (—)-trans-p-menthane-3,8-diol in 2-2.5 and 4-4.5% amount have been found. The catnip sample deriving from Germany contained a small amount of anetol, citronellol, neral, geraniol and geranial (6-13%), and possibly two isomers of nepetalactone in 23-31%.
The Nepeta parviflora endemic in the Nagyvolgy valley near Nagykaracsony consisted of the same compounds in the investigated years (1998-2000). Its limonene, methyl chavicol, b-cariophyllene, b-selinene, b-cubebene, davanone, germacrene-D constituents have been identified. In the year 2000 different GC % of these compounds were detected in the different organs of the plants.
The closely related species Nepeta cataria var. citriodora contained 83% citral, and the N. glechoma (= Glechoma hederacea) contained 41% a-cubebene, 20% patchoulenol, 7,7% spathulenol respectively. These compounds were identified by gas chromatography and gas chromatography / mass spectrometry.
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Effect of the placement of self-incompatible apricot varieties on their yield in commercial plantations
82-86.Views:296Earlier studies concerning self-, free- and cross- fertilization of apricot varieties grown in Hungary, proved the existence of self-sterile as well as self-fertile varieties within the recommended assortment. The self-sterile and partially self-fertile varieties should be planted in association with polliniser varieties, only. The present paper reports about the yields of trees of the widely grown, self-sterile local variety, Ceglédi óriás (Giant of Cegléd), depending on the distance of adequate polliniser trees. In the univarietal, 27 row-wide block of the relevant variety, an efficient polliniser, Magyar kajszi was planted to the 10th and 19th row. In the close vicinity, another block of polliniser, Rózsakajszi C. 320 was located. The number of fruits set per tree has been counted or estimated in two consecutive years. In both seasons, the yield of the Ceglédi óriás trees diminished with the growing distance from the nearest polliniser trees. Those trees in the center of the block, between the two (10th and 19th) rows of Magyar kajszi bore acceptable yield (40 kg/tree in 1987), however, considerable reduction of the number of the fruits set was stated already in the 4-5th row from the polliniser away. Similar gradient of fruit set was apparent in relation to the neighbouring block of Rózsakajszi C 320. The beneficial effect of the vicinity of polliniser varieties was obvious as far as the distance of the 10th row. Taking into consideration the self-sterility, the early blooming time and the poor fertilization of the variety Ceglédi óriás, a planting design of associating it with at least two polliniser varieties (e.g. Gönci magyar kajszi and Ceglédi bíbor) is highly recommended. On the basis also of earlier results, a proposal has been developed for the association of apricot varieties as recommendations for optimising yields. Blooming time, fertilizing potential, schedule of the picking season and market possibilities have to be considered simultaneously.